Дисертації з теми "RET Gene"
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Hofstra, Robert Martinus Wouter. "The RET gene and its associated diseases." [S.l. : [Groningen] : s.n.] ; [University Library Groningen] [Host], 1995. http://irs.ub.rug.nl/ppn/142201383.
Повний текст джерелаCarter, Melissa Terry. "Characterization of the mouse RET gene and a cross-species comparison of RET isoforms." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ63279.pdf.
Повний текст джерелаLee, King-yiu. "The Ret gene in the enteric nervous system expression analysis and generation of ret deficient mice /." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31449669.
Повний текст джерелаLee, King-yiu, and 李景耀. "The Ret gene in the enteric nervous system: expression analysis and generation of ret deficient mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31449669.
Повний текст джерелаGuo, Kexiao. "DNA Secondary Structures in the Promoters of Human VEGF and RET Genes and Their Roles in Gene Transcriptional Regulation." Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/195943.
Повний текст джерелаSantos, Marina Silva dos. "Genetic susceptibility to thyroid cancer: contributions of RET polymorphisms." Master's thesis, Universidade da Beira Interior, 2012. http://hdl.handle.net/10400.6/1199.
Повний текст джерелаThyroid cancer is the most common malignancy of the endocrine system, represents more than 1% of all malignancies and has an estimated annual incidence of 212,000 cases worldwide. The term differentiated thyroid carcinoma (DTC) comprises the subtypes papillary thyroid carcinoma (PTC) and follicular thyroid carcinomas (FTC), these subtypes represent the two most common subtypes of thyroid cancer (approximately 80% and 10% respectively). Despite its incidence DTCs have a good prognosis with relatively few metastases and deaths associated. The polymorphisms (variants in DNA sequence among individuals that have a frequency of at least 1% in a population) of RET proto-oncogene have been studied in different populations for association with susceptibility to thyroid cancer, but with inconsistent findings mainly in DTC. To clarify the contribution of single locus or haplotypes (polymorphisms that are transmitted through generations as a unit) of RET polymorphisms to genetic susceptibility to DTC among Portuguese patients, we conducted a case–control study by analyzing four well-characterized RET polymorphisms (G691S, L769L, S836S and S904S). To achieve this aim, the RET polymorphisms were genotyped and haplotype frequencies were estimated in a population of 282 individuals with DTC and in a control population of 254 individuals. Allele, genotype and haplotype distributions were compared among cases and controls. Patient population was subdivided according to several clinical parameters and allele, genotype and haplotype distributions were compared among the subgroups. The single locus analysis showed an overrepresentation of the S836S polymorphism in patients when compared to controls. Also the heterozygous genotypes of the G691S/S904S polymorphisms were overrepresented in cases diagnosed after the age of 45 years and the heterozygous genotype of G691S polymorphism revealed an overrepresentation in patients with tumors larger then 10mm of diameter at diagnosis. The haplotype analysis showed an overrepresentation of GGTC haplotype in patients particularly in those diagnosed after the age of 45 years. In conclusion, our data suggest that the S836S polymorphism may be associated with increased risk of DTC. Also the heterozygous genotype of the G691S/S904S polymorphisms seems to be associated with age of onset of DTC and additionally the heterozygous genotype of G691S polymorphism appeared to be in association with tumor size. Finally, one haplotype appears to be associated with increased risk of DTC particularly in those developed in later age (after the age of 45 years). These findings need to be confirmed by larger studies in order re-evaluate the role of these variants in the susceptibility to DTC.
Le, Hir Hervé. "Etude de l'epissage alternatif des arn pre-messagers du gene ret et du gene de la tyrosine hydroxylase dans les pheochromocytomes." Paris 7, 1998. http://www.theses.fr/1998PA077089.
Повний текст джерелаLa, Perle Krista Marie DuBray. "Characterization of ret/PTC1 Transgenic-p53 knockout mice and sodium/iodide symporter gene transfer for Prostate Cancer /." The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486457871785739.
Повний текст джерелаMarsee, Derek K. "Exploration of novel therapies for thyroid cancer adenoviral gene therapy and 17-allylamino-17-demethoxygeldanamycin /." Connect to this title online, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1087497053.
Повний текст джерелаTitle from first page of PDF file. Document formatted into pages; contains xv, 118 p.; also includes graphics (some col.) Includes bibliographical references (p. 106-118). Available online via OhioLINK's ETD Center
Pasini, Andrea. "Bases biologiques des néoplasies endocriniennes multiples de type 2 et de la maladie de Hirschsprung : étude des conséquences fonctionnelles des mutations du gène RET." Lyon 1, 1997. http://www.theses.fr/1997LYO1T249.
Повний текст джерелаTong, Qiang. "Molecular mechanism of the activation of the ret/ptc1 oncogene in papillary thyroid carcinomas and characterization of the promoter of the rat sodium iodide symporter gene /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487947501136078.
Повний текст джерелаLau, Ming-fung Anson, and 劉銘豐. "Regulation of gene expression by NF-kB and STATs downstream of RET receptor tyrosine kinase in Hirschsprung's disease and thyroid cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B29976911.
Повний текст джерелаHatanaka, Roxanne. "Rastreamento de variantes de significado desconhecido (VUS) no gene RET em indivíduos-controle e em pacientes com carcinoma medular de tireoide." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-24022016-111259/.
Повний текст джерелаIntroduction: Multiple endocrine neoplasia type 2 (MEN-2) is a tumor syndrome with autosomal dominant inheritance, in which tumors are associated with medullary thyroid carcinoma (MTC), pheochromocytoma (FEO) and primary hyperparathyroidism (HPT). This syndrome occurs due to activating mutations in the RET proto-oncogene, which lead to constitutive activation of tyrosine kinase signaling pathways that deregulate the cell cycle. According to the International Consensus on MTC/MEN-2 of 2001 and 2009 one should recommend that RET mutation carriers, including asymptomatic individuals, should undergo prophylactic total thyroidectomy (TT), increasing the chance of cure of the disease. It is not recommended clinical screening in patients that show only isolated polymorphisms (non-pathogenic variant). However, there are individuals who carry genetic variants of unknown clinical significance (VUS), generating doubt about the best clinical management. Currently, there is no consistent knowledge whether these variants may or may not be involved with the increased risk to MTC. The present project has approached the several aspects of these VUS, such as the allele frequency, in silico pathogenic prediction, published data and public databases, in order to increase our knowledge about VUS, in an attempt to contribute by offering appropriate clinical management to VUS carriers. Objective: To expand the knowledge of the pathogenic potential of some of the VUS of the RET gene, focusing especially on the controversial genetic variant p.Y791F. Methods: We performed the mutation screening of hotspots exons of the RET gene of DNA samples of 2061 adult/elderly healthy individuals and of patients with CMT by Sanger sequencing and Next Generation Sequencing (NGS) techniques. Pathogenic predictions of the studied variants were generated using six genetic softwares. Allelic frequency of RET VUS was assessed in different public databanks. Results: Genetic screening of control samples identified the presence of p.Y791N, p.Y791F and p.E511K germline variants. Patients with MTC carrying p.V648I and p.K666N germline variants were localized and family members were screened and clinically investigated. In addition, a new case with pheochromocytoma was found to carry the p.Y791F germline variant. The in silico analyses showed that 4 out of 6 packages were more informative, suggesting physico-chemical structure alteration caused by 25 out of 48 RET VUS. Very low allele frequencies were found in the public databases including healthy individuals and tumor samples. In vitro studies have been performed only for 15 out of 48 RET VUS. Conclusion: Our data strongly suggest that the p.Y791F variant, when occurring in an isolated form, is a benign polymorphism not associated with increased risk of MTC. Conversely, its co-occurrence with bona fide RET mutations as C634Y may lead to modulation of the phenotype, as increasing the frequencies of large and bilateral pheochromocytomas in MEN2A families. Family members carrying the p.V648I variant isolate have been followed clinically for approximately 15 years. As no indication of MCT, pheochromocytoma or hyperparathyroidism development has been documented, we conclude that this variant is a rare RET benign polymorphism. More information is needed to a better characterization of other VUS as E511K, K666N and Y791N. Thus, carriers with these variants should be necessarily examined through a periodic clinical follow up
Lamazière, Frédéric. "Formes familiales de néoplasie endocrinienne multiple 2A : intérêt du dépistage des mutations du gène RET, à propos d'un cas familial." Bordeaux 2, 1995. http://www.theses.fr/1995BOR2M179.
Повний текст джерелаRaouane, Mouna. "Vectorisation de siRNA dirigés contre l'oncogène de fusion RET/PTC1 impliqué dans le carcinome papillaire de la thyroïde par des nanoparticules de squalène." Thesis, Paris 11, 2011. http://www.theses.fr/2011PA11T077.
Повний текст джерелаThe papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy. This tumour is associated with somatic mutations of the RET proto-oncogene, due to gene rearrangements of the proto-RET. RET/PTC1 rearrangement is the most common genetic alteration identified to date, it is formed by an intra chromosomic rearrangement which leads to the juxtaposition of the RET Tyrosine Kinase domain of the proto-RET with the gene H4. The fusion RET/PTC1 oncogene represents an interesting target for small interfering RNA (siRNA) strategies since it is present only in the tumour cells and not in the surrounding normal cells. However, the biological efficacy of the siRNAs is hampered by their short plasma half-life due to poor stability in biological fluids and low intracellular penetration. In order to protect siRNA from degradation, and to improve their intracellular capture, we applied the concept of “squalenoylation”, ie. The bioconjugation of a drug substance to squalene, for the delivery of siRNA targeted toward the RET/PTC1 fusion oncogene. The acyclic isoprenoid chain of squalene was covalently coupled with RET/PTC1 siRNA at the 3’-terminus of the sense strand via a stable thioether linkage. The linkage of RET/PTC1 siRNA to squalene leads to an amphiphilic molecule that self-organise in water as RET/PTC1 siRNA-SQ nanoassemblies of 170 nm and Zeta potential of -26.4 mV. These RET/PTC1 siRNA-SQ NPs did not showed any cytotoxicity in vitro. Interestingly, in vivo, in a mouse xenografted RET/PTC1 experimental model, RET/PTC1 siRNA-SQ nanoparticles inhibited tumour growth, RET/PTC1 oncogene and oncoprotein expression, after intravenous injections of 2.5 mg/kg cumulative dose. In the last of this work, GALA-cholesterol combination with siRNA-SQ NPs further enhanced nucleic acid internalization, promoted their escape into the cytosol and consequently their gene silencing efficiency in vitro. In conclusion, these results showed that the “squalenoylation” offers a new non cationic plate-form for the siRNA delivery
Boichard, Amélie. "Caractérisation moléculaire des formes métastatiques de carcinome médullaire de la thyroïde." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA11T015/document.
Повний текст джерелаMedullary thyroid carcinoma (MTC) is a rare neuroendocrine tumor, arising from calcitonin-secreting cells. This cancer occurs in a family context in a third of cases. All inherited forms and nearly 40% of sporadic forms are caused by activating point-mutations in the RET oncogene, coding for a tyrosine-kinase receptor. Other oncogenic events causing sporadic cases remain unclear, but activating mutations of RAS oncogenes have been discovered recently.Prognosis of MTC is essentially linked to early lymph node occurrence. Initial surgery of metastatic forms is often insufficient and patients are considered in therapeutic dead-end. The recent advent of selective tyrosine-kinase inhibitors (TKIs) has brought a new impetus to the management of refractory tumors, some of them targeting the RET receptor. Optimization of these treatments require improving knowledge of the underlying molecular mechanisms of tumor development.In this context and helped by a large collection of human specimens, we have sought to deepen the description of genomic landscape of MTC.At first, we evaluated the structural and chromosomal abnormalities presented by MTC. We showed, by optimizing sequencing methods, that RET and RAS mutations are involved in over 96% of the cases, these events are mutually exclusives. These mutations can distinguish several groups of aggressiveness and of response to TKI treatments. We also observed, by comparative genomic hybridization techniques, recurrent abnormalities such as deletion of the short arm of chromosome 1 and loss of entire chromosomes 4 and 22. These losses appear to be early events of tumorigenesis MTC.In a second step, we determined - by a microarray approach – the microRNA expression profile of MTC. Some of these post-transcriptional regulators seem related to tumor invasiveness, such as miR-21, miR-199 and miR-129. We demonstrated the potential of microRNAs miR-21 and miR-199 as circulating diagnosis biomarkers of MTC. The functional impact of the precursor forms mir-21 and mir-129 was then evaluated by transfection in TT and MZ- CRC1 cellular models.Observations obtained pave the way for a lot of new potential studies. They allow the definition of tissue biomarkers distinguishing metastatic forms or refractory patients. Finally, they highlight new pathways for the discovery of additional therapeutic targets in this disease
Sekiya, Tomoko. "Análise do gene CDKN1B/p27kip1 em pacientes com neoplasia endócrina múltipla tipo 2." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-26022014-112355/.
Повний текст джерелаINTRODUCTION: In Multiple Endocrine Neoplasia type 2 (MEN2) the development of medullary thyroid carcinoma (MTC), pheochromocytoma (PHEO) and primary hyperparathyroidism (HPT) are associated with activating germline mutations in RET proto-oncogene. Cases of sporadic MTC may have somatic RET mutations (~ 40%). The phenotypic variability observed in cases with familial MTC/MEN2 and PHEO/MEN2 indicates the probable involvement of additional genetic events that could be responsible for the clinical differences observed in the affected individuals (age development, progression and aggressiveness of the tumor). Other genetic alterations such as RET double mutations, SNPs and specific haplotypes may influence susceptibility, aggressiveness and MEN2 phenotype modulation. However, studies of other genes involved in the tumorigenesis of MEN2 are still in progress. Recently, it was shown that the activated RET controls the expression of cell cycle inhibitory proteins (p18 and p27). Germline mutations in the p27 gene have recently been associated with the susceptibility to neuroendocrine tumors and are associated with the MEN4 syndrome (Multiple endocrine neoplasia type 4). Somatic inactivating mutations p27 are rarely found in many types of tumors. However, several studies have documented that reduced expression and subcellular location of p27 is controlled by post-transductional changes and/or epigenetic factors. OBJECTIVES: This study aimed to evaluate the role of genes recently associated with RET activated in tumors from MEN2 patients and also check whether polymorphisms in the p27 gene would be acting as modulators of phenotype in a large MEN2 family. PATIENTS: We analyzed 66 tumor samples from 36 patients with clinical and genetic diagnosis of MEN2 and from 28 individuals belonging to a large family with FMTC/MEN2A and RET C620R mutation. METHODS: The analyses of somatic p27, p15, p18 and RET were performed by PCR and direct sequencing of DNA and microsatellite analysis was performed for p27 by PCR and capillary electrophoresis. Expression analysis and subcellular localization of p27 protein were performed by Western blot and immunohistochemistry. The analysis of phenotype modulation in MEN2A families was performed by the amplification of exon 1 of the p27 gene in a whole blood sample. RESULTS: There were no somatic mutations in the p27 gene and also in the p15 and p18 genes. However, we verified a low p27 protein expression in MTC/MEN2 and PHEO/MEN2 that showed a definite correlation with the type and aggressiveness of the mutated RET codon, mainly in those tumors from cases with germline RET codon 634 mutations (control vs 634, p=0,05; control vs 634/791, p= 0,032; 620 vs 634, p=0,045; 620 vs 634/791, p= 0,002; 620 vs 634 + 634/791, p=0,036). It was also verified a positive correlation between the immunohistochemistry expression of nuclear p27 subcellular location and the p27 p.V109G TT genotype (p=0,03). CONCLUSIONS: The reduction in the expression of p27 and its subcellular localization are likely to be associated with somatic changes in other genes that control the processes of phosphorylation of p27 protein through post-transductional events
Lioupis, Alexandros. "Studies on non-primate growth hormones : molecular evolution and structure-function relationships." Thesis, University of Sussex, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263869.
Повний текст джерелаShoja, Valia. "A Broad Analysis of Tandemly Arrayed Genes in the Genomes of Human, Mouse, and Rat." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/35800.
Повний текст джерелаMaster of Science
Banister, Susan H. "Regenerating gene (reg) expression : studies in the BB rat and man." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296263.
Повний текст джерелаMcSweeny, Andrew J. "Identification of Candidate Genes within Blood Pressure QTL Containing Regions Using Gene Expression Data." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1212501779.
Повний текст джерелаAllan, G. "Gene expression during keratinocyte differentiation." Thesis, University of Newcastle Upon Tyne, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233424.
Повний текст джерелаZablocki, Destinee Elizabeth. "Differential Expression of Calsarcin Genes in Orthognathic Surgery Patients with ACTN3 R577X Gene Deviations." Master's thesis, Temple University Libraries, 2016. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/405298.
Повний текст джерелаM.S.
Objective: Malocclusion is a complex musculoskeletal trait, with muscle playing an integral role in vertical facial development. A single nucleotide polymorphism (SNP) produces the R577XX nonsense mutation in the alpha-actinin-3 (ACTN3) gene, creating a stop codon and loss of its protein. With loss of ACTN3, alpha-actinin-2 (ACTN2) is upregulated. Calsarcins, known inhibitors of calcineurin activation, preferentially bind ACTN2 leading to a surge in free calcineurin. The increase in calcineurin activity produces the phenotypic shift of fast muscle fibers toward the slow myogenic program seen in the ACTN3 null genotype (Seto et al., 2013). Here, we have tested whether calsarcin gene expression is affected by ACTN3 genotypes in human masseter muscle. Methods: Subjects undergoing orthodontic treatment and orthognathic surgery were recruited from the University of Lille, Department of Oral and Maxillofacial Surgery in Northern France. During the bilateral sagittal split osteotomy, masseter muscle samples were collected from the discarded section of deep anterior superficial masseter muscle, snap frozen, and shipped to Dr. Sciote’s lab at Temple University. RNA from masseter muscle samples was isolated from 41 subjects using TRIzolTM reagent. MYOZ gene expression was quantified by RT-PCR using an adult skeletal muscle reference standard (commercially prepared skeletal muscle RNA; Ambion, Inc), and individual primer-probe sets for MYOZ1, MYOZ2, MYOZ3, and HPRT1 (utilized for normalization of data). ANOVA and unpaired t-tests were used to determine the significance of expression differences between MYOZ genes and by ACTN3 R577X genotypes, as well as by malocclusion classes. Pearson analyses were used to determine correlations between MYOZ expression and fiber type mean percent occupancies. Results: The main aim of this project was to determine whether expression of the three calsarcin genes, MYOZ1, 2, and 3, differs between subjects with RR, RX and XX genotypes for the ACTN3 gene, as well as between sagittal and vertical classes of malocclusion, asymmetries and TMD. Differences were found for MYOZ3 expression where relative quantities in males, but not females, decreased progressively from the ACTN3 RR, to RX, and XX genotypes. Among subjects with the RX genotype, expression differed significantly between males and females by an unpaired t-test. A statistically significant difference was detected between MYOZ2 and Class II, Class III malocclusions (p=0.05). Sagittal differences were compared further by ANOVA analyses with a statistically significant difference detected for MYOZ3 with a probability of 0.02. Correlation analyses comparing fiber type mean % occupancy with calsarcin gene expression revealed a significant positive relationship between MYOZ2 and type I (slow-twitch) fibers. Correspondingly, a significant correlation of MYOZ2 expression with type IIA and IIX (fast-twitch) fibers was negative. Conclusions: The greatest relative quantity of RNA for the three calsarcin genes was found in MYOZ3, suggesting more calsarcin-3 may be needed in masticatory muscle structure and function than other calsarcin isoforms. Alternatively, high expression of MYOZ3 in the masseter samples may indicate that there are relatively greater amounts of that isoform in cranial muscle than in the limb skeletal muscle standard used in these studies. Also, relative quantities of MYOZ3 expression in males decreased progressively from the ACTN3 RR, to RX, and XX genotypes. While this data may suggest that the ACTN3 R577X polymorphism may affect MYOZ3 expression in males of the malocclusion patient population, an increased sample of male subjects would be needed to determine if this trend has true significance. Expression of MYOZ2 (calsarcin-1) was strongly correlated with slow fiber-type occupancy in masseter muscle of our patient population. The muscle-specific expression of each calsarcin may lend to the understanding of this result. MYOZ2 is the only isoform found in both cardiac muscle and slow-twitch skeletal muscle, while MYOZ1 and MYOZ3 are both found in skeletal muscle with a predilection towards fast-twitch skeletal muscle (Frey et al., 2004).
Temple University--Theses
Johnson, Rory. "Molecular mechanisms of REST-mediated repression of RE1 target genes in neural cells." Thesis, University of Leeds, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435796.
Повний текст джерелаClark, Allan F. "Disruption of the Ren-1d gene." Thesis, University of Edinburgh, 1997. http://hdl.handle.net/1842/13405.
Повний текст джерелаTRONCHE, FRANCOIS. "Regulation de la transcription des genes specifiques de tissus : le promoteur du gene de l'albumine du rat." Paris 7, 1992. http://www.theses.fr/1992PA077199.
Повний текст джерелаPiton, Nicolas. "Optimisation de la prise en charge diagnostique, pronostique et théranostique des carcinomes broncho-pulmonaires humains : des techniques d’imagerie in vivo à la biologie moléculaire. Ligation -dependent RT-PCR : a new specific and low-cost technique to detect ALK, ROS and RET rearrangements in lung adenocarcinoma A new assay for detection of theranostic gene translocations and MET exon 14 skipping in thoracic oncology. One-year perspective routine LD-RT-PCR in 413 newly diagnosed lung tumors STK11 mutations are associated with lower PDL1 expression in lung adenocarcinoma BRAF V600E mutation is not always present as expected ! A case report of lung and thyroid carcinomas A novel method for in vivo imaging of solitary lung nodules using navigational bronchoscopy and confocal laser microendoscopy." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR119.
Повний текст джерелаLung cancer is a serious and frequent condition for which the management strategies have been dramatically modified in recent years, from a diagnostic, prognostic and “theranostic” perspective, most notably with the introduction of “targeted therapies”. The latter have demonstrated dramatic improvement in both quality of life and survival rates of eligible patients, yet consequently highlight new complications in diagnosis, treatment options or technical considerations which can be attributed to the growing number of molecular alterations to be detected from limited tissue samples frequently encountered in thoracic oncology. This work combines 5 different research papers from 2 different angles: prognostic and “theranostic” molecular markers of lung cancer, as well as in vivo diagnostic procedures of lung cancer. The first angle encompasses 4 articles. The first two evaluate a new molecular technique, LD-RT-PCR, to detect gene translocation in lung cancer. The third article explores the association between STK11 mutations in lung cancer and the expression of PDL1. Finally, the fourth article is a case report illustrating the importance of a morphological approach to lung cancer. The second angle compares in vivo imaging techniques by endoscopy using confocal laser microendoscopy alongside a conventional microscopic approach
Brug, Marcel Patrick van der. "Excitotoxic induced gene expression in the adult rat brain /." [St. Lucia, Qld.], 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17077.pdf.
Повний текст джерелаAbankwa, Daniel. "Axotomy induced gene expression in rat brain." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963458051.
Повний текст джерелаMcDowell, Ian L. "Studies on the rat phenylalanine hydroxylase gene." Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260390.
Повний текст джерелаDuran, Alonso Maria Beatriz. "Genetic mapping of the rat agu gene." Thesis, University of Glasgow, 1997. http://theses.gla.ac.uk/39021/.
Повний текст джерелаHunt, Jannine M. "A psbA phylogeny for selected rhodophyceae /." Electronic version (PDF), 2006. http://dl.uncw.edu/etd/2007-2/huntj/janninehunt.pdf.
Повний текст джерелаScott, Ian Stuart. "Regulation of hormone gene expression in normal and mutant rodents." Thesis, University of Oxford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258240.
Повний текст джерелаZemmel, Rodney W. "Rev-RRE interactions in human immunodeficiency virus gene expression." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390234.
Повний текст джерелаAsfour, Boulos. "Experimental design for cardiac gene therapy : rat heart transplantation models and gene transfer techniques /." Münster : Schüling, 2000. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=008939687&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Повний текст джерелаAu, Mun-yee Deborah. "Molecular studies of rat [beta]-globin gene cluster." Click to view the E-thesis via HKUTO, 1996. http://sunzi.lib.hku.hk/hkuto/record/B31234598.
Повний текст джерелаDenny, Paul. "Molecular analysis of gene expression in rat brain." Thesis, Imperial College London, 1991. http://hdl.handle.net/10044/1/46744.
Повний текст джерелаNasa, Zeyad, and nasa zeyad@med monash edu au. "Characterization of the Rat Relaxin-like Factor Gene." RMIT University. Medical Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080514.100729.
Повний текст джерелаNorgate, Zoe. "Gene expression in rat uterine natural killer cells." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614884.
Повний текст джерела區敏宜 and Mun-yee Deborah Au. "Molecular studies of rat {221}-globin gene cluster." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1996. http://hub.hku.hk/bib/B31234598.
Повний текст джерелаTrickett, Jeffrey Ianto. "The arylacetamide deacetylase gene in rat and mouse." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397619.
Повний текст джерелаRees, D. "Characterisation of the rat phenylalanine hydroxylase gene promotor." Thesis, University of Liverpool, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343614.
Повний текст джерелаAu, Mun-yee Deborah. "Molecular studies of rat b-globin gene cluster /." Hong Kong : University of Hong Kong, 1996. http://sunzi.lib.hku.hk/hkuto/record.jsp?B18995779.
Повний текст джерелаMcCormick, James A. "Transcriptional regulation of the rat glucocorticoid receptor gene." Thesis, University of Edinburgh, 2000. http://hdl.handle.net/1842/23114.
Повний текст джерелаCraig, Nicola Jane. "Genetic and physical mapping of the rat agu locus." Thesis, University of Glasgow, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341722.
Повний текст джерелаDavies, Janet Elizabeth. "Towards a transgenic rat model of Familial Neurohypophysial Diabetes Insipidus." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247860.
Повний текст джерелаMontgomery, Douglas S. "An investigation of rat DNA polymerase alpha." Thesis, University of Aberdeen, 1985. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU362670.
Повний текст джерелаCosta, Marcella Motta da. "Duplicação do gene MECP2 em meninos com deficiência intelectual." reponame:Repositório Institucional da UnB, 2016. http://dx.doi.org/10.26512/2016.02.D.20076.
Повний текст джерелаA Deficiência Intelectual (DI), é um grande problema de saúde pública, visto que sua prevalência é de aproximadamente 2%-3%. Sua maior prevalência em homens se deve ao grande número de formas de DI Ligada ao cromossomo X (DILX). Essa podem ser, classificadas como sindrômicas (S-DILX) ou não-sindrômicas (NS-DILX). Uma das causas mais comuns de DILX em meninos é a duplicação do gene MECP2. Estudos anteriores relatam frequências de 1 a 15% de portadores de CNVs incluindo o gene MECP2 em coortes de meninos com DI. Este trabalho buscou rastrear a presença de microduplicações do gene MECP2 em uma população de 265 pacientes do sexo masculino, sendo 138 analisados pela técnica de qPCR e 127 pacientes provenientes do banco de dados de análise cromossômica por microarray do laboratório de Genética da UnB. Não foram identificados CNVs do gene MECP2 nos 265 pacientes estudados. A frequência de CNVs de MECP2 nesse estudo foi menor que a reportada anteriormente na literatura e sua baixa prevalência não justifica a triagem individual de alterações de número de cópias de MECP2 em meninos com DI.
The Intellectual Disability (ID) is a big problem of public health, since its prevalence is approximately 1%. Its higher prevalence in men is due to the large number of forms of ID linked to the X chromosome (DILX). It can be classified as syndromic (S-DILX) or non-syndromic (NS-DILX). One of the most common causes of DILX in boys is the duplication of the MECP2 gene. Earlier studies reported frequencies from 1 to 15% of CNVs carriers including MECP2 in cohort of boys with ID. This study aimed to trace the presence of microduplications of the MECP2 gene in a population of 265 male patients, 138 analysed by qPCR technique and 127 patients from the chromosomal analysis database by microarray from the Genetics Laboratory at UnB. We have not identified none of the MECP2 gene in 265 patients studied. The frequency of CNVs from MECP2 in this study was lower than reported previously in the literature and its low prevalence does not justify the individual triage of the alteration of MECP2 number of copies in boys with ID.
Tang, Mei Kuen. "Applications of the subtractive hybridization method to study gene expression in rat liver after cadmium exposure." HKBU Institutional Repository, 1994. https://repository.hkbu.edu.hk/etd_ra/39.
Повний текст джерелаRodrigues, Lucas Mateus Rivero [UNESP]. "Avaliação da agressividade e caracterização genética de linhagens de Ralstonia Solanacearum isoladas de diferentes plantas hospedeiras." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/97187.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O presente trabalho teve como objetivo avaliar a agressividade de linhagens de Ralstonia solanacearum provenientes de solanáceas, plantas ornamentais e eucalipto, em plantas de batata, tomate e fumo, bem como caracterizar as linhagens por meio de técnicas moleculares. Vinte e duas linhagens foram utilizadas nos ensaios de avaliação da agressividade, em experimentos conduzidos em casa-de-vegetação evidenciaram alta severidade da doença pelas linhagens de R. solanacearum quando inoculadas em plantas de tomate e batata, sendo a batata mais afetada nas inoculações. Todas as linhagens mostraram-se agressivas, sendo que o fumo mostrou baixa suscetibilidade ao ataque das bactérias. As linhagens mais agressivas em plantas de tomate foram IBSBF 309, IBSBF 1712, IBSBF 1839, IBSBF 1882, IBSBF 1883 e IBSBF 2000, pertencentes às biovares I, II e III. As linhagens mais agressivas às plantas de fumo foram IBSBF 309, IBSBF 2131 e IBSBF 292T, pertencentes à biovar I. Foi efetuado também ensaio de microbiolização in vitro em sementes de eucalipto, a fim de se identificar possíveis linhagens patogênicas a esta espécie vegetal e concluiu-se que todas as linhagens utilizadas infectaram plantas de eucalipto ou afetaram seu crescimento. A caracterização molecular de 41 linhagens de Ralstonia solanacearum, provenientes de diversas plantas hospedeiras, incluindo solanáceas, bananeira, helicônia, plantas ornamentais e eucalipto, foi efetuada empregando-se ERIC e BOX-PCR e os resultados mostraram grande diversidade genética entre as linhagens. A análise de PCR-RFLP da região espaçadora 16S-23S DNAr permitiu distinguir os isolados pertencentes à biovar III das demais biovares (I, II, IIA e IIT), quando digeridos com as enzimas Taq I e Hin6 I. A análise de sequenciamento de parte dos genes Endoglucanase (Egl) e MutS possibilitou a classificação em filotipos e os resultados...
This study aimed to evaluate the aggressiveness of strains of Ralstonia solanacearum from solanaceus, ornamental and eucalyptus plants, on potato, tomato and tobacco, and to characterize the strains through molecular techniques. Twenty-two strains were used in this study to evaluate the aggressiveness and, the experiments conducted in a greenhouse revealed the high susceptibility of tomato and potato plants, with the potato being the most affected on through the inoculations. All isolates proved to be aggressive and higher tolerance to the attack of bacteria was verified on tobacco plants. Strains more aggressive on tomato were IBSBF 309, IBSBF 1712, IBSBF 1839, IBSBF 1882, IBSBF 1883 and IBSBF 2000, belonging to biovars I, II and III. The more aggressive strains on the tobacco plants were IBSBF 309, IBSBF 292T and IBSBF 2131 belonging to biovar I. Tests in vitro of microbiolization of eucalyptus seeds were also performed in order to identify possible pathogenic strains to this species and the results showed that all strains used cause infection on emerging plants or affected their growth. To molecular characterization of 41 strains of Ralstonia solanacearum from several host plants including solanaceous, banana, heliconia, ornamentals and eucalyptus were employed to ERIC and BOX-PCR, and the results showed high genetic diversity among strains. The analysis of PCR-RFLP of 16S-23S spacer region rDNA allowed us to distinguish the isolates belonging to biovar III from the others (biovars I, II, IIA and IIT) when digested with enzymes Taq I and Hin6 I. The sequence analysis of the partial of Endoglucanase (Egl) and MutS genes allowed the classification in phylotypes and the results revealed a predominance of the phylotype II in Brazil, and four isolates were classified in the phylotype I, all belonging to biovar III