Статті в журналах: "Resin purification"

1

Li, Tuo Ping, Su Hong Li, Wei Na Fu, Yang Sun, You Feng Jia, Zhong Sheng Zhao, and Ru Gang Zhu. "Purification of Soyasaponin with Macroporous Resin." Advanced Materials Research 396-398 (November 2011): 1379–81. http://dx.doi.org/10.4028/www.scientific.net/amr.396-398.1379.

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The conditions for purification of soyasaponin by macroporous resin adsorption strategies were analyzed. The results showed that macroporous resin D3520 was a suitable resin for the purification of soyasaponin. Static adsorption assay showed that 20:1 (w/w) D3520/soyasaponin at 40 oC for 2h adsorption were optimal for soyasaponin purification. In the column chromatography, 0.5BV (bed volume)/h flow rate would be suitable to reach higher purity of near 90 % soyasaponin.

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2

Talpur, Marvi Kanwal. "Highly Selective Purification of Grewia asiatica Anthocyanin Based on Macroporous Resins." Pakistan Journal of Analytical & Environmental Chemistry 22, no. 1 (June 23, 2021): 44–52. http://dx.doi.org/10.21743/pjaec/2021.06.06.

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In the present study, enactments and separation characteristics of five extensively utilized adsorbents for enhancement and purification of Grewia asiatica Anthocyanins (ACNs) extracts were analysed. Among five tested resins (AB-8, Sepabeads Sp-700, C18SPE Cartridge, Sepabeads Sp-207 and AB-80), AB-8 resin exhibited the best adsorbent ability for Grewia asiatica ACNs (84.24mg/mL resin). Results of static adsorption tests revealed that AB-8 resin selected for kinetics and isotherm experiments followed a pseudo 2nd order model along with Langmuir isotherm. In order to improve operational procedure, dynamic adsorption and desorption tests were done on a packed column of AB-8 resin. Optimum factors for subsequent adsorption-desorption experiments; processing volume 20ml, flow rate 2 mL/min with elution solvent of acidified methanol (1%v/v) were used. HPLC and LC-MS/MS profiles of the purified extract confirmed seven ACNs in Grewia asiatica samples, out of which cyanidin-3-O-(6"acetylglucoside) comprises 44-63% (695 μg/g) of total ACNs composition. Moreover, pigment purification using AB-8 resin did not alter ACNs mixture composition after purification but enhance the peak intensity and gives effective purification. Hence present work reveals that the separation procedure established through column chromatography providing an effective methodology to enhance the purification of ACNs from Grewia asiatica.

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3

Li, Di-Hua, Yan Wang, Yuan-Shan Lv, Jun-Hong Liu, Lei Yang, Shu-Kun Zhang, and Yu-Zhen Zhuo. "Preparative Purification of Liriodendrin fromSargentodoxa cuneataby Macroporous Resin." BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/861256.

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The preparative purification of liriodendrin fromSargentodoxa cuneatausing macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

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4

Usui, Kenji, Shin-ichiro Yokota, Kazuya Iwata, and Yoshio Hamada. "Novel Purification Process for Amyloid Beta Peptide(1-40)." Processes 8, no. 4 (April 15, 2020): 464. http://dx.doi.org/10.3390/pr8040464.

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Amyloid beta peptide (Aβ)-related studies require an adequate supply of purified Aβ peptide. However, Aβ peptides are “difficult sequences” to synthesize chemically, and low yields are common due to aggregation during purification. Here, we demonstrate an easier synthesis, deprotection, reduction, cleavage, and purification process for Aβ(1-40) using standard 9-fluorenylmethyloxycarbonyl (Fmoc)-protected amino acids and solid-phase peptide synthesis (SPPS) resin [HMBA (4-hydroxymethyl benzamide) resin] that provides higher yields of Aβ(1-40) than previous standard protocols. Furthermore, purification requires a similar amount of time as conventional purification processes, although the peptide must be cleaved from the resin immediately prior to purification. The method described herein is not limited to the production of Aβ(1-40), and can be used to synthesize other easily-oxidized and aggregating sequences. Our proposed methodology will contribute to various fields using “difficult sequence” peptides, such as pharmaceutical and materials science, as well as research for the diagnosis and treatment of protein/peptide misfolding diseases.

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5

Bi, Yong Guang, and Yao Quan Tan. "Study on Macroporous Resin Separation and Purification of Total Flavonoids of Plantago Process." Advanced Materials Research 550-553 (July 2012): 987–92. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.987.

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To study the macroporous resin separation and purification of total flavonoids of Plantago optimum conditions, the static adsorption and desorption experiments by filtering out the best macroporous resin, and plantain extract for the assessment of total flavonoid content indicators in the single-factor test based on the orthogonal design, the amount of resin in the hole, eluent concentration, alcohol consumption and other factors for the study to optimize the AB-8 macroporous resin separation and purification of total flavonoids plantain best process conditions. The results show that: the effect of various factors affecting the size of the order of separation and purification has to: eluent concentration> ethanol consumption> macroporous resin usage, optimization of the optimum conditions: resin dosage is 1 times the amount of crude drug, the use of eluent volume fraction of 40% ethanol, elution agent is 12.5 times the amount of crude drug. Verify the conditions in this test, three repeated experiments were similar, the average flavonoid content 0.2127%, the transfer rate of 90.57%, indicating that the scientific and rational and stable process conditions can be effectively used plantain purified flavonoids.

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6

Khandelwal, Payal. "Resin Built for Large-Scale Purification." Genetic Engineering & Biotechnology News 38, no. 6 (March 15, 2018): 18–19. http://dx.doi.org/10.1089/gen.38.06.07.

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7

Arthapersada, Reno Imam, Muhammad Kurniawan Adiputra, Indra P. Hakim, Imam Karfendi Putro, Asep P. Zainuddin, Lisendra Marbelia, and Ahmad Tawfiequrahman Yuliansyah. "Optimasi Biaya dalam Proses Pemurnian Metanol untuk Mengurangi Resin sebagai Limbah Bahan Berbahaya dan Beracun di PT Kaltim Methanol Industri." Jurnal Rekayasa Proses 14, no. 2 (December 31, 2020): 148. http://dx.doi.org/10.22146/jrekpros.59553.

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Purification process of raw methanol from its impurities to produce pure methanol at PT. Kaltim Methanol Industri (PT KMI) is carried out by several steps, including degassing, distillation, and adsorption. One of the impurities, tri methyl amine (TMA), could be removed by adding NaOH. Another method to remove TMA is conducted by adsorption process on ion exchange resin on the vessel called TMA catchpot. The TMA catchpot performance is very crucial in methanol purification process. Thus, monitoring and optimization are required to be performed regularly. Once the TMA catchpot resin has exhausted, the performance will be drop and methanol purification could not be done efficiently. Furthermore, the ion exchange resin should be replaced with new resin. This study evaluates the performance of the TMA catchpot during the charge of 2010, 2012, and 2016, calculates the NaOH consumption during operational time, and optimizes the cost. Resin regeneration option was introduced and compared with the conventional method (i.e. resin replacement). Economic evaluation shows that the lowest annual cost could be obtained by fresh resin replacement every 4 years and resin regeneration every 2 years. Resin regeneration option gives not only annual cost reduction, but also positive impact to the environment, by decreasing the amount of hazardous waste (i.e. spent resin) significantly.Keywords: ion exchange resin; methanol purification; regeneration; tri methyl amineA B S T R A KProses pemurnian metanol mentah (raw) dari pengotornya untuk menghasilkan metanol murni di PT. Kaltim Methanol Industri (PT KMI) dilakukan melalui beberapa tahapan antara lain degassing, distilasi dan adsorpsi. Salah satu zat pengotor adalah tri methyl amine (TMA) yang dapat dihilangkan dengan penambahan NaOH. Metode lain untuk menghilangkan TMA adalah dengan proses adsorpsi menggunakan resin penukar ion di dalam tangki yang disebut TMA catchpot. Performa TMA catchpot sangat penting dalam proses pemurnian metanol. Oleh karena itu, pemantauan dan optimalisasi perlu dilakukan secara berkala. Setelah resin pada TMA catchpot jenuh, performanya akan menurun dan pemurnian metanol tidak dapat dilakukan secara efisien. Selanjutnya, resin penukar ion harus diganti dengan resin baru. Artikel ini mengevaluasi kinerja catchpot TMA pada penggantian resin (charge) 2010, 2012 dan 2016, menghitung konsumsi NaOH sebagai fungsi waktu operasi, dan mengoptimasi biaya pemurnian. Selain itu, disimulasikan opsi regenerasi resin, sebagai pembanding metode konvensional (penggantian resin). Evaluasi ekonomi menunjukkan bahwa biaya tahunan paling rendah didapatkan dengan penggantian resin baru setiap 4 tahun, dan regenerasi resin setiap 2 tahun. Selain biaya tahunan yang rendah, regenerasi ini berdampak positif terhadap lingkungan dengan mengurangi timbulan limbah B3 (resin bekas) secara signifikan.Kata kunci: pemurnian metanol; regenerasi; resin penukar ion; tri metil amin

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8

Kartika, Ika Amalia, Oky Tresia Ordian Bernia, Illah Sailah, Tirto Prakoso, and Yohanes Aris Purwanto. "A binary solvent for the simultaneous Calophyllum oil-resin extraction and purification." Research in Agricultural Engineering 65, No. 2 (July 2, 2019): 63–69. http://dx.doi.org/10.17221/30/2018-rae.

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Sustainable biodiesel production can be realised by the use of a low-cost feedstock, efficient energy and renewable raw materials. The simultaneous Calophyllum oil-resin extraction and its purification using a binary solvent (n-hexane mixed with alcohol) were examined to meet those aspects. The extraction conditions effect was investigated to determine the optimal oil yield and quality. n-Hexane mixed with alcohol was extracted and purified effectively the oil from Calophyllum seeds. The oil yield and its quality were mainly affected by the n-hexane-to-alcohol ratio. The oil yield enhanced as the n-hexane-to-alcohol ratio enlarged from 1:1 to 2.5:1. The acid value and density of the oil improved as the n-hexane-to-alcohol ratio declined from 2.5:1 to 1:1. The n-Hexane-to-alcohol ratio of 2.5:1 provided the best yield (59%) of the oil extracted at 40°C for 5 hours. The oil presented its best quality at 0.893 g·cm<sup>–3</sup> of density, 41.0 mPa·s of viscosity, 8.8 mg KOH·g<sup>–1</sup> of the acid value, 88.3 g per 100 g of the iodine value, < 1% of moisture content and < 0.04% of ash content. The oil also had an inhibitory activity against Staphylococcus aureus.

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9

Ma, Jian Wei, and Ya Rui Song. "Purification Raw Water by Magnetic Resin (MIEX)." Advanced Materials Research 726-731 (August 2013): 3185–88. http://dx.doi.org/10.4028/www.scientific.net/amr.726-731.3185.

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The objective of this research was to compare enhanced coagulation with anion exchange for removal of natural organic matter (NOM) and bromide. Treatment with a magnetic ion exchange resin (MIEX) was the primary focus of this study. The performance of the magnetic ion exchange resin,MIEX, in the treatment of raw water was investigated. MIEX can effectively remove UV-absorbing substances DOC. The removal of organic substances is accompanied by the elimination of other undesirable components, such as nitrogen and phosphorus. The optimal process parameters are at resin doses of 5-10 mL L1and contact time of 10-15 min, as determined via jartests. Based on this study, MIEX treatment is a suitable and efficient pretreatment method for the removal of extra dissolved organic matters and nitrates in raw water .

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10

Xu, Li Ping, Xin Wang, and Peng Cheng Yao. "Study on Technology of Isolation and Purification of Corn Germ Glutathione with Macroporous Resin." Advanced Materials Research 183-185 (January 2011): 306–9. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.306.

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Study on Macroporous resin purified glutathione of corn germ , determine the best conditions for separation and purification as follows: glutathione and resin volume ratio 4:1, flow rate 1 mL / min ,adsorption time 50 min, elution and resin volume ratio of 4:1, analysis time 120 min, recovery rate of glutathione78.95 %.

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11

Alliot, C., N. Audouin, A. C. Bonraisin, V. Bossé, J. Laizé, C. Bourdeau, B. M. Mokili, N. Michel, and F. Haddad. "82Sr purification procedure using Chelex-100 resin." Applied Radiation and Isotopes 74 (April 2013): 56–60. http://dx.doi.org/10.1016/j.apradiso.2012.10.020.

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12

Jin, Xue Yuan, Hong Liu, and San Fu Zhu. "Optimization for Purification Technology of Platycodins by Macroreticular Resin." Advanced Materials Research 781-784 (September 2013): 852–55. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.852.

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In order to purify the platycodins from Platycodon grandiflorum, AB-8 macroporous resin was used to test. Based on single factor experiments, orthogonal test was used to optimum the purification conditions. Adsorption pH, adsorption temperature, adsorption time and platycodins concentration were as factors and adsorption capacity was as index. The results were as follows: adsorption pH 6.0, adsorption temperature 40°C, adsorption tim110min, platycodins concentration 2.0mg/mL were the optimization conditions.The adsorption quantity reached at 39.1mg/g. So AB-8 macroporous resin was a suitable resin for purify the platycodins from Platycodon grandiflorum.

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13

Zhen, Yuanhang, Tao Zhang, Bo Jiang, and Jingjing Chen. "Purification and Characterization of Resistant Dextrin." Foods 10, no. 1 (January 18, 2021): 185. http://dx.doi.org/10.3390/foods10010185.

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In this study, an efficient method for the purification of resistant dextrin (RD) using membrane filtration and anion exchange resin decolorization was developed, then the purified RD was characterized. In the membrane filtration stage, suspended solids in RD were completely removed, and the resulting product had a negligible turbidity of 2.70 ± 0.18 NTU. Furthermore, approximately half of the pigments were removed. Static decolorization experiments revealed that the D285 anion exchange resin exhibited the best decolorization ratio (D%), 84.5 ± 2.03%, and recovery ratio (R%), 82.8 ± 1.41%, among all the tested resins. Under optimal dynamic decolorization conditions, the D% and R% of RD were 86.26 ± 0.63% and 85.23 ± 0.42%, respectively. The decolorization efficiency of the D285 resin was superior to those of activated carbon and H2O2. Moreover, the chemical characteristics and molecular weight of RD did not change significantly after purification. The nuclear magnetic resonance spectroscopy of RD showed the formation of new glycosidic linkages that are resistant to digestive enzymes. The superior water solubility (99.14%), thermal stability (up to 200 °C), and rheological properties of RD make it possible to be widely used in food industry.

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14

Ferreira, Marcionila O., Elisa M.B.D. Sousa, and Camila G. Pereira. "Purification of Crude Glycerine Obtained from Transesterification of Cottonseed Oil." International Journal of Chemical Reactor Engineering 11, no. 1 (July 5, 2013): 385–92. http://dx.doi.org/10.1515/ijcre-2012-0071.

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Abstract The objective of this study was to purify glycerine obtained from the transesterification of cottonseed oil, using ion exchange resins. The batch process used strong cation, weak anion and a mixture of its resins. The purified glycerine was characterized as to metal content, colour and glycerol content. The experiments were conducted using the resins individually and in series. In the experiments in series, there was a constant decrease in conductivity. Analyses without the use of charcoal showed that conductivity behaved similarly to that of treatment with charcoal. For conductivity tests using activated charcoal and individual resins, the mixed resin produced the best result when compared to commercial glycerine.Considering the analysis made with activated carbon, when the glycerine solution was treated with individual resins, it was observed that the conditions established for treatment with 10 g of resin, 5 hours of contact with each resin and 50 mL of glycerine, its conductivity decreased to the cation exchange resin, increased to anionic resin and had a variable value with respect to the mixed resin. In serial processing, there was a steady decrease in the conductivity of the glycerine solution. The content of glycerol, after the solution had been passed through activated charcoal, 56% of the compound was recovered. It was also observed that the mixed resin retained a lower amount of glycerol. It can be concluded that ion exchange resins were efficient in treating the glycerine solution in all the tests, since there was little glycerol retention and high undesirable compound retention.

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15

Hebert, Mathieu, Emmanuel Serra, Eugène Vorobiev, and Houcine Mhemdi. "Isolation and Purification of Mustard Glucosinolates by Macroporous Anion-Exchange Resin: Process Optimization and Kinetics’ Modelling." Processes 10, no. 2 (January 18, 2022): 191. http://dx.doi.org/10.3390/pr10020191.

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Glucosinolates (GSL) (β-thioglucoside-N-hydroxy sulfates) are rich-sulfur secondary metabolites raising potential biofumigation interest due to their biological properties. Sinigrin and gluconapin are the main glucosinolates present in brown mustard seeds (Brassica juncea). These glucosinolates are very suitable for the development of phytosanitary products due to their fungicidal, bactericidal and insecticidal effects. In this work, the purification of sinigrin and gluconapin extracted from defatted mustard seeds was studied using macroporous anion exchange resins. A strongly and a weakly anionic resin were first tested according to the nature of their functional group and through their selectivity towards glucosinolates. Anion-exchange resin purification was first studied in static (batch) mode in order to determine the optimal operating conditions; it was then tested in a dynamic (continuous) mode (column) to validate the process. In static mode, the adsorption behavior and characteristics of both resins were compared. The results showed that the strongly basic resin PA312LOH ensures better adsorption of glucosinolates and that the experimental data fit well with the Freundlich isotherm. Moreover, analysis showed that PA312LOH resin was selective for glucosinolates purification towards the proteins. The desorption of glucosinolates was then investigated. Firstly, the operating conditions were optimized by studying the effects of salt concentration and the eluate-resin ratio. This preliminary optimization allowed recovering 72.9% of intact sinigrin and the juice purity was increased from 43.05% to 79.63%. Secondly, dynamic (continuous mode) experiments allowed the recovery of 64.5% of sinigrin and 28% of gluconapin by varying the eluent ionic strength and the flow rate. Resin was finally successfully regenerated using NaOH.

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16

Lu, Cheng, Jack Bevers, Tulika Tyagi, Hao To, May Lin, Shu Ti, Gerry Nakamura, et al. "AviTrap: A novel solution to achieve complete biotinylation." PLOS ONE 19, no. 4 (April 25, 2024): e0297122. http://dx.doi.org/10.1371/journal.pone.0297122.

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Site specific biotinylation of AviTagged recombinant proteins using BirA enzyme is a widely used protein labeling technology. However, due to the incomplete biotinylation reactions and the lack of a purification method specific for the biotinylated proteins, it is challenging to purify the biotinylated sample when mixed with the non-biotinylated byproduct. Here, we have developed a monoclonal antibody that specifically recognizes the non-biotinylated AviTag but not the biotinylated sequence. After a ten-minute incubation with the resin that is conjugated with the antibody, the non-biotinylated AviTagged protein is trapped on the resin while the fully biotinylated material freely passes through. Therefore, our AviTrap (anti-AviTag antibody conjugated resin) provides an efficient solution for enriching biotinylated AviTagged proteins via a simple one-step purification.

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17

Wang, Xiaoxia, Zhou Zhang, Yun Wang, Yayi Wu, Li Miao, Yue Ma, Lihua Wei, Wen Chen, and Hong Li. "Enrichment of Total Flavonoids and Licochalcone A from Glycyrrhiza inflata Bat. Residue Based on a Combined Membrane–Macroporous Resin Process and a Quality-Control Study." Molecules 29, no. 10 (May 12, 2024): 2282. http://dx.doi.org/10.3390/molecules29102282.

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Glycyrrhiza inflata Bat. produces a lot of licorice waste after water extraction, which also retains abundant total flavonoids (TFs) and licochalcone A. However, licorice residue is often wasted due to the lack of good utilization of resources in practical applications. This study first screened the optimal membrane pore size and resin type and then explored the mechanism and conditions of the adsorption of TFs on the resin. Then, different combinations and sequences of membrane and macroporous resin (MR) methods were investigated. It was found that using the membrane method for initial purification, followed by the MR method for further purification, yielded the best purification results. Next, response surface methodology was utilized to investigate the resin’s dynamic desorption conditions for TFs. Finally, the TF purity increased from 32.9% to 78.2% (2.38-fold) after purification by a combined membrane–MR process; the purity of licochalcone A increased from 11.63 mg·g−1 to 22.70 mg·g−1 (1.95-fold). This study verified the feasibility of enriching TFs and licochalcone A from licorice residue using a membrane–MR coupling method. In addition, a quality-control method was established using a fingerprinting method on the basis of ultrahigh-performance liquid chromatography (UPLC) to ensure the stability of the enrichment process.

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18

Li, Hui, Jiayu Lin, Baoqing Bai, Tao Bo, Yufei He, Shanhong Fan, and Jinhua Zhang. "Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves." Molecules 28, no. 21 (October 26, 2023): 7273. http://dx.doi.org/10.3390/molecules28217273.

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The flavonoids from Perilla leaves were extracted using flash extraction assisted by ultrasonic extraction with ethanol. Subsequently, macroporous resin was employed for the isolation and purification of these flavonoids, followed by an investigation into their antioxidant activity. The process conditions for the extraction of flavonoids from Perilla leaves were designed and optimized using a one-way experiment combined with a response surface methodology. The optimal extraction conditions were determined as follows: the liquid–solid ratio was 20:1, ethanol volume fraction of 60%, ultrasound temperature of 60 °C, ultrasound time of 10 min and flash evaporation time of 60 s. The optimal extraction rate of flavonoids is 9.8 mg/g. In terms of separation and purification, a high-performance macroporous resin (HPD450 resin) with high purification efficiency was selected through static analysis and adsorption experiments. The optimal enrichment conditions were as follows: loading concentration of 0.06 mg/mL, optimal loading concentration of 20 mL, elution concentration of 70% and 76 mL, providing a reference for the further development and utilization of Perilla leaf flavonoids.

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19

Hou, Mengyang, Wenzhong Hu, Zhilong Xiu, Aili Jiang, Lei Men, Kexin Hao, Xingsheng Sun, and Duo Cao. "Preparative Purification of Total Flavonoids from Sophora tonkinensis Gagnep. by Macroporous Resin Column Chromatography and Comparative Analysis of Flavonoid Profiles by HPLC-PAD." Molecules 24, no. 17 (September 3, 2019): 3200. http://dx.doi.org/10.3390/molecules24173200.

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For the full development and utilization of Sophora tonkinensis Gagnep., this study was primarily intended to established a simple and efficient approach for the preparative purification of total flavonoids from S. tonkinensis by macroporous resin column chromatography (MRCC). The adsorption and desorption characteristics of the total flavonoids on ten macroporous resins were first studied, and AB-8 resin was chosen as the most suitable, and the adsorption data were best fitted to the pseudo-second-order kinetics model and Langmuir isotherm model. Furthermore, the technological parameters for the purification of the total flavonoids were optimized using column chromatography. After a sample one-step purification procedure, the content of the total flavonoids increased by about 4.76-fold from 12.14% to 57.82%, with a recovery yield of 84.93%. In addition, the comparative analysis of the flavonoid extracts before and after purification was performed by high-performance liquid chromatography coupled with photodiode-array detection (HPLC-PAD). The results showed that the contents of six major flavonoids in the purified product were all higher than before the purification. Therefore, the AB-8 MRCC established in this work was a promising method for the industrial-scale purification of the total flavonoids from S. tonkinensis.

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20

Liu, Yan-Bo, Wang Yao, Shuai Meng, Xiang-Bao Meng, Zhong-Jun Li, and Qing-Hua Lou. "A novel cobalt(0) alkyne complex assisted “capture and release” strategy for oligosaccharide rapid assembly." Organic Chemistry Frontiers 5, no. 13 (2018): 2098–102. http://dx.doi.org/10.1039/c8qo00324f.

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21

Dong, Wanyuan, and Yifeng Li. "CH1-specific affinity resins possess the potential of separating heterodimer from homodimers in asymmetric bispecific antibody purification." Journal of Biological Methods 11, no. 3 (September 3, 2024): e99010020. http://dx.doi.org/10.14440/jbm.2024.0026.

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CaptureSelect CH1-XL and Praesto 70 CH1 are two affinity media that specifically bind to the CH1 domain of an antibody. In the current work, we first demonstrated that these two CH1-specific affinity media bound to different monoclonal antibodies (mAbs) with varied strengths under identical conditions. We previously had observed the same on a Protein L-conjugated resin and showed that such a property could facilitate homodimer removal in asymmetric bispecific antibody (bsAb) purification. Next, using Praesto 70 CH1, we showed that a small difference in binding between two mAbs could be significantly exaggerated by adding sodium chloride to the mobile phase, further demonstrating this resin can potentially play a role in bsAb purification. Finally, with a concrete bsAb case study, we showed that, like Protein L, Praesto 70 CH1 could separate the target heterodimer from the homodimer by-product. Homodimers are common product-related impurities associated with the recombinant production of asymmetric bsAbs, which can be difficult to remove. Their removal, even a partial one, at the capture stage is a big advantage as it can alleviate the purification burden on subsequent polishing steps and render the overall process more robust. Therefore, Praesto 70 CH1’s unique property is highly desirable, and this affinity resin can be a better alternative than Protein A for product capture in asymmetric bsAb purification.

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Loibl, S. F., Z. Harpaz, R. Zitterbart, and O. Seitz. "Total chemical synthesis of proteins without HPLC purification." Chemical Science 7, no. 11 (2016): 6753–59. http://dx.doi.org/10.1039/c6sc01883a.

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23

Younes, Ali, Cyrille Alliot, Jafar Sunga Ali, Anne-Cécile Bonraisin, Marcel Mokili, Steffen Happel, Aude Bombard, Ferid Haddad, and Gilles Montavon. "Production of polonium from bismuth and purification using TBP resin and Sr resin." Journal of Radioanalytical and Nuclear Chemistry 324, no. 2 (March 16, 2020): 823–28. http://dx.doi.org/10.1007/s10967-020-07109-5.

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24

Li, Shangyong, Linna Wang, Xuehong Chen, Mi Sun, and Yantao Han. "Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification." Marine Drugs 17, no. 1 (January 21, 2019): 68. http://dx.doi.org/10.3390/md17010068.

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Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics. Therefore, a rapid and efficient one-step affinity purification procedure plays important roles in screening native chitosanases. In this study, we report the design and synthesis of affinity resin for efficient purification of native chitosanases without any tags, using chitodisaccharides (CHDS) as an affinity ligand, to couple with Sepharose 6B via a spacer, cyanuric chloride. Based on the CHDS-modified affinity resin, a one-step affinity purification method was developed and optimized, and then applied to purify three typical glycoside hydrolase (GH) families: 46, 75, and 80 chitosanase. The three purified chitosanases were homogeneous with purities of greater than 95% and bioactivity recovery of more than 40%. Moreover, we also developed a rapid and efficient affinity purification procedure, in which tag-free chitosanase could be directly purified from supernatant of bacterial culture. The purified chitosanases samples using such a procedure had apparent homogeneity, with more than 90% purity and 10–50% yield. The novel purification methods established in this work can be applied to purify native chitosanases in various scales, such as laboratory and industrial scales.

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25

Westcott, Jay Y., Kirk M. Maxey, Jim MacDonald, and Sally E. Wenzel. "Immunoaffinity Resin for Purification of Urinary Leukotriene E4." Prostaglandins & Other Lipid Mediators 55, no. 5-6 (April 1998): 301–21. http://dx.doi.org/10.1016/s0090-6980(98)00027-6.

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26

Xu, Jie, Jiawen Zhu, Kui Chen, Yanyang Wu, and Jiji Gu. "ENRICHMENT AND PURIFICATION OF BITESPIRAMYCIN USING MACROPOROUS RESIN." Chemical Engineering Communications 199, no. 10 (October 2012): 1320–33. http://dx.doi.org/10.1080/00986445.2012.682322.

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27

Porter, C. E., F. D. Riley, R. D. Vandergrift, and L. K. Felker. "Fermium Purification Using Teva™ Resin Extraction Chromatography." Separation Science and Technology 32, no. 1-4 (January 1997): 83–92. http://dx.doi.org/10.1080/01496399708003188.

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28

Matos, Tiago, João A. Queiroz, and Leif Bülow. "Plasmid DNA purification using a multimodal chromatography resin." Journal of Molecular Recognition 27, no. 4 (February 10, 2014): 184–89. http://dx.doi.org/10.1002/jmr.2349.

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29

Anvari, Masoumeh, and Gholam Khayati. "Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins." Polish Journal of Chemical Technology 18, no. 1 (March 1, 2016): 127–32. http://dx.doi.org/10.1515/pjct-2016-0019.

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Abstract The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal array (OA) experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin) on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6%) and the lowest (1.3%) contribution, respectively. The results showed that the Taguchi method is able to model the purification of rebaudioside A process well (R2 > 0.998) and can therefore be applied in future studies conducted in various fields. Adsorption temperature 35°C, desorption time 60min, elution solution ratio 3, adsorption volume 200ml and HPD-400 as resin were the best conditions determined by the Taguchi method.

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30

Chen, Xusheng, Qin Li, Honggang He, Jianhua Zhang та Zhonggui Mao. "Effect of ion form of the ion-exchange resin on ε-poly-l-lysine purification from microbial fermentation broth". RSC Advances 9, № 21 (2019): 12174–81. http://dx.doi.org/10.1039/c9ra00493a.

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31

Stapleton, IW. "A simple method of polyamine purification." Australian Journal of Chemistry 38, no. 4 (1985): 633. http://dx.doi.org/10.1071/ch9850633.

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A simple procedure for the large-scale purification of commercial polyethyleneamines (H2N[CH2CH2NH]nH where n = 2-5) is described in which the per- tosylate salt separates as a crystalline solid from aqueous solution. The salts require no further purification except for pentaethylenehexamine (n = 5), which requires recrystallization from water. The free bases are regenerated from the tosylate salt by an anion-exchange resin.

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32

Corbett, Derek B., Changyoung Hong, Richard Venditti, Hasan Jameel, and Sunkyu Park. "Hydrophobic resin treatment of hydrothermal autohydrolysate for prebiotic applications." RSC Advances 9, no. 55 (2019): 31819–27. http://dx.doi.org/10.1039/c9ra06018a.

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33

Mundodi, Suparna, Porecha Rishi, Anderson Murray, and Rapicavoli Nicole. "FPLC in a pipette tip (P3297)." Journal of Immunology 190, no. 1_Supplement (May 1, 2013): 211.6. http://dx.doi.org/10.4049/jimmunol.190.supp.211.6.

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Abstract Molecular characterization of the immune response and how this process is implemented requires the purification of native proteins and antibodies. There are numerous purification methods to isolate proteins of interest, but these methods usually require multiple steps to attain the level of purity required for most studies. The Rainin PureSpeed Protein Purification System utilizes pipette tips containing purification resin at their distal end interfaced with an E4 XLS pipette to simplify enrichment procedures for virtually all proteins. The E4 XLS pipette, when set up within a 96-deepwell plate, is able to carry out semi-automated purification of antibodies and other native or recombinant proteins. The pipette is able to drive up-and-down sample flow over the immobilized resin matrix for as many as 12 samples in parallel. The ability of the pipette to carry out liquid handling steps enhances laboratory productivity by allowing an investigator to step away from the apparatus until the next step in the protocol. This new, innovative technology from Rainin Instrument is available with pipette tips containing protein A, protein G, Ni-IMAC, or one of four different ion exchange resins, which allow for multiple options for protein purification. Finally, the PureSpeed System functions well in advanced applications such as protein and chromatin immunoprecipitation, offering speed and versatility relative to agarose and magnetic bead technologies.

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34

Wen, Yao, Huaguo Chen, Xin Zhou, Qingfang Deng, Chao Zhao, and Xiaojian Gong. "A polyamide resin based method for adsorption of anthocyanins from blackberries." New Journal of Chemistry 40, no. 4 (2016): 3773–80. http://dx.doi.org/10.1039/c6nj00054a.

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35

Arakawa, Tsutomu, Yui Tomioka, Masataka Nakagawa, Chiaki Sakuma, Yasunori Kurosawa, Daisuke Ejima, Kouhei Tsumoto, and Teruo Akuta. "Non-Affinity Purification of Antibodies." Antibodies 12, no. 1 (February 13, 2023): 15. http://dx.doi.org/10.3390/antib12010015.

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Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography.

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36

Zhao, Tao, Lin Ma, Jing Jun Du, Liang Zhang, and Xiao Yan Wu. "Purification of Hulless Barley Anthocyanins with Macroporous Resins." Advanced Materials Research 236-238 (May 2011): 2701–4. http://dx.doi.org/10.4028/www.scientific.net/amr.236-238.2701.

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The purification effects of six macroporous adsorption resins on total anthocyanins of hulless barley were compared. The results showed that XAD7HP was the best resin for purification of hulless barley anthocyanins due to its excellent adsorption and desorption capability. The optimal technological parameters were: the pH of sample solution was 3.0, the concentration of sample solution was 21.6 mg/L, the adsorption flow velocity was 1.0 mL/min, the eluting velocity is 1.0 mL/min, and the eluent was about 6.6 bed volumes of 80% ethanol. Reutilization test showed that the XAD7HP resin could be used repeatedly with no significant change of adsorption rate (P>0.05). After purified with XAD7HP, the color value of hulless barley anthocyanins was 41.1, increasing 9.1 times than that of raw extraction.

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37

Barozzi, Annalisa, R. Ashton Lavoie, Kevin N. Day, Raphael Prodromou, and Stefano Menegatti. "Affibody-Binding Ligands." International Journal of Molecular Sciences 21, no. 11 (May 27, 2020): 3769. http://dx.doi.org/10.3390/ijms21113769.

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While antibodies remain established therapeutic and diagnostic tools, other protein scaffolds are emerging as effective and safer alternatives. Affibodies in particular are a new class of small proteins marketed as bio-analytic reagents. They feature tailorable binding affinity, low immunogenicity, high tissue permeation, and high expression titer in bacterial hosts. This work presents the development of affibody-binding peptides to be utilized as ligands for their purification from bacterial lysates. Affibody-binding candidates were identified by screening a peptide library simultaneously against two model affibodies (anti-immunoglobulin G (IgG) and anti-albumin) with the aim of selecting peptides targeting the conserved domain of affibodies. An ensemble of homologous sequences identified from screening was synthesized on Toyopearl® resin and evaluated via binding studies to select sequences that afford high product binding and recovery. The affibody–peptide interaction was also evaluated by in silico docking, which corroborated the targeting of the conserved domain. Ligand IGKQRI was validated through purification of an anti-ErbB2 affibody from an Escherichia coli lysate. The values of binding capacity (~5 mg affibody per mL of resin), affinity (KD ~1 μM), recovery and purity (64–71% and 86–91%), and resin lifetime (100 cycles) demonstrate that IGKQRI can be employed as ligand in affibody purification processes.

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38

Wang, Yong, Yu Bo Wang, Wen Ge Zhang, Lin Zhou, Ning Ning Wang, Cong Yang Yan, and Xiao Li. "Identification and Separation of the Novel Antifungal Antibiotic of Streptomyces Hygroscopicus BOS-013 Strain by Simulated Moving Bed Chromatography." Advanced Materials Research 485 (February 2012): 245–48. http://dx.doi.org/10.4028/www.scientific.net/amr.485.245.

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BOS-013 actinomycete strain was obtained by separating difference soil of areas from ChangBai Mountain. The antifungal antibiotic produced by Streptomyces Hygroscopicus BOS-013 was firstly purified by means of macro-porous adsorbent resin and thin-layer chromatography. Separation and Purification of the antifungal antibiotic from its fermentation broth of streptomyces hygroscopicus BOS-013 strain were further carried out by Simulated Moving Bed Chromatography and then the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of micro-porous adsorbent resin and detection by high performance liquid chromatography were established. The exact structure of the antibiotic was identified by mass spectrometry and NMR spectra.

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39

Song, Ilchan, Yang Joo Kang, Su-Lim Choi, Dalmuri Han, Deuk-Su Kim, Hae Kyung Lee, Joon-Chul Lee, Jeanho Park, Do-Sun Kim, and Kisung Ko. "Purification of plant-derived anti-virus mAb through optimized pH conditions for coupling between protein A and epoxy-activated beads." PeerJ 7 (May 21, 2019): e6828. http://dx.doi.org/10.7717/peerj.6828.

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The main goal of this research was to determine optimum pH conditions for coupling between protein A and epoxy-activated Sepharose beads for purification of monoclonal antibodies (mAbs) expressed in plants. To confirm the effect of pH conditions on purification efficacy, epoxy-activated agarose beads were coupled to protein A under the pH conditions of 8.5, 9.5, 10.5, and 11.5 (8.5R, 9.5R, 10.5R, and 11.5R, respectively). A total of 300 g of fresh leaf tissue of transgenic Arabidopsis expressing human anti-rabies mAb (mAbP) SO57 were harvested to isolate the total soluble protein (TSP). An equal amount of TSP solution was applied to five resin groups including commercial protein A resin (GR) as a positive control. The modified 8.5R, 9.5R, 10.5R, and 11.5R showed delayed elution timing compared to the GR control resin. Nano-drop analysis showed that the total amount of purified mAbPSO57 mAbs from 60 g of fresh leaf mass were not significantly different among 8.5R (400 μg), 9.5R (360 μg), 10.5R (380 μg), and GR (350 μg). The 11.5R (25 μg) had the least mAbPSO57. SDS–PAGE analysis showed that the purity of mAbPSO57 was not significantly different among the five groups. Rapid fluorescent focus inhibition tests revealed that virus-neutralizing efficacies of purified mAbPSO57 from all the five different resins including the positive control resin were similar. Taken together, both pH 8.5 and 10.5 coupling conditions with high recovery rate should be optimized for purification of mAbPSO57 from transgenic Arabidopsis plant, which will eventually reduce down-stream cost required for mAb production using the plant system.

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40

Hay, Iain D., Jinping Du, Natalie Burr, and Bernd H. A. Rehm. "Bioengineering of Bacteria To Assemble Custom-Made Polyester Affinity Resins." Applied and Environmental Microbiology 81, no. 1 (October 24, 2014): 282–91. http://dx.doi.org/10.1128/aem.02595-14.

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ABSTRACTProof of concept for thein vivobacterial production of a polyester resin displaying various customizable affinity protein binding domains is provided. This was achieved by engineering various protein binding domains into a bacterial polyester-synthesizing enzyme. Affinity binding domains based on various structural folds and derived from molecular libraries were used to demonstrate the potential of this technique. Designed ankyrin repeat proteins (DARPins), engineered OB-fold domains (OBodies), and VHHdomains from camelid antibodies (nanobodies) were employed. The respective resins were produced in a single bacterial fermentation step, and a simple purification protocol was developed. Purified resins were suitable for most lab-scale affinity chromatography purposes. All of the affinity domains tested produced polyester beads with specific affinity for the target protein. The binding capacity of these affinity resins ranged from 90 to 600 nmol of protein per wet gram of polyester affinity resin, enabling purification of a recombinant protein target from a complex bacterial cell lysate up to a purity level of 96% in one step. The polyester resin was efficiently produced by conventional lab-scale shake flask fermentation, resulting in bacteria accumulating up to 55% of their cellular dry weight as polyester. A further proof of concept demonstrating the practicality of this technique was obtained through the intracellular coproduction of a specific affinity resin and its target. This enablesin vivobinding and purification of the coproduced “target protein.” Overall, this study provides evidence for the use of molecular engineering of polyester synthases toward the microbial production of specific bioseparation resins implementing previously selected binding domains.

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41

Meile, Kristine, and Aivars Zhurinsh. "Preparative Solid Phase Extraction for the Purification of Levoglucosan Obtained from Lignocellulose." Key Engineering Materials 721 (December 2016): 82–86. http://dx.doi.org/10.4028/www.scientific.net/kem.721.82.

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Automated preparative scale solid phase extraction (SPE) has been used to separate levoglucosan – a valuable platform chemical from the liquid products of wood pyrolysis. The sorbent for SPE was a strongly basic anion exchange resin in OH-form. Separation of levoglucosan could be done with water as the eluent, the regeneration of the resin was done with a NaCl solution. Up-scaling the purification of levoglucosan is a step forward industrial production of this chemical.

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42

Zhao, Chan, Jian Xu, Yao Liu, Peng Xu, Jin Yi, Lin Feng, Yanyan Miao, and Yongping Zhang. "Extraction and Purification of Flavonoids and Antiviral and Antioxidant Activities of Polygonum perfoliatum L." Molecules 30, no. 1 (December 25, 2024): 29. https://doi.org/10.3390/molecules30010029.

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The aim of the present study was to optimize the process parameters for the extraction and purification of total flavonoids from Polygonum perfoliatum L., in addition to analyzing their chemical composition and evaluating their activity against varicella-zoster virus (VZV) and antioxidant activity. The optimum extraction process was determined using one-way and response surface methods with the following conditions: ethanol concentration of 82.00%, temperature of 90.29 °C, solid-to-liquid ratio of 1:32.78 g/mL, extraction time of 1.5 h, and two extractions with a yield of 14.98 ± 0.11 mg/g. Purification was then carried out using D101 macroporous resin to obtain a flavonoid purity of 43.00 ± 2.55%, which was 3.38 times higher than that of the crude extract (12.74 ± 1.04%). Further purification was carried out using a 1:9 hybrid column of macroporous resin and polyamide, and the purity of flavonoids was enhanced to 59.02 ± 2.23%, which is 1.37 times higher than that of the macroporous resin purifier (43.00 ± 2.55%) and 4.63 times higher than that of the crude extract (12.74 ± 1.04%). Seventy-nine flavonoids were identified using ultra-performance liquid chromatography-tandem high-resolution mass spectrometry (UPLC-HRMS). In addition, the purified flavonoids showed good anti-VZV and antioxidant activities. Therefore, this study can provide technical support and theoretical basis for the further development and utilization of Polygonum perfoliatum L. resources.

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43

Wang, Jiong Ran, Yan Zhou Hu, Xiao Han, and Ke Ding. "Separation and Purification of Zizyphusine, Spinosin, and 6′′′-Feruloylspinosin From Zizyphi Spinosi Semen." Natural Product Communications 15, no. 3 (March 2020): 1934578X2091055. http://dx.doi.org/10.1177/1934578x20910556.

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The methods of extraction, separation, and purification of zizyphusine, spinosin, and 6′′′-feruloylspinosin from Zizyphi Spinosi Semen ( ZSS) were investigated. From 31.2 g defatted ZSS, 47.7 mg zizyphusine, 57.8 mg spinosin, and 80.5 mg 6′′′-feruloylspinosin were obtained after ultrasonic extraction, purification with macroporous resin, separation by flash chromatography, and purification by high-pressure preparative chromatography. The purities of zizyphusine, spinosin, and 6′′′-feruloylspinosin were 98.6%, 98.2%, and 99.0%, respectively, and their yields 85.0%, 82.8%, and 83.0%. The methods provide stable samples of these compounds for further study of their physiological function and application.

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44

Zhang, Wei-Wei, та Perry Churchill. "Purification of D-β-hydroxybutyrate dehydrogenase from rat brain". Biochemistry and Cell Biology 68, № 6 (1 червня 1990): 980–83. http://dx.doi.org/10.1139/o90-144.

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D-β-Hydroxybutyrate dehydrogenase (BDH), a lipid-requiring enzyme, has been purified to homogeneity from rat brain using a new improved method. The purified rat brain BDH has a subunit molecular mass of 31 kilodaltons on sodium dodecyl sulphate – polyacrylamide gel electrophoresis. The apoenzyme, i.e., the enzyme devoid of phospholipid, has no activity, but can be activated by phospholipid to a specific activity of 125 μmol/(min∙mg). This is 625-fold greater than the activity in the mitochondrial fraction. The new purification procedure involves chromatography using a quaternary amine Sepharose resin followed by a sulphonate Sepharose resin, and eliminates the need for glass bead adsorption chromatography.Key words: mitochondria, membrane, phospholipid, dehydrogenase, lipid dependent.

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45

Zhang, Qing-An, Dong-Dong Wu, and Chen-Xi Wei. "Purification of Amygdalin from the Concentrated Debitterizing-Water of Apricot Kernelsusing XDA-1 Resin." Processes 7, no. 6 (June 10, 2019): 359. http://dx.doi.org/10.3390/pr7060359.

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In this study, six macroporous resins were screened on their adsorption and de-adsorption characteristics for the amygdalin in the debitterizing wastewater concentrate (DWC). The results indicate that the XDA-1 resin exerts good adsorption and de-adsorption capacities on the amygdalin. In order to further confirm its feasibility, the factors affecting the capacity of adsorption and de-adsorption, and its adsorption mechanisms were also investigated. The results suggest that the optimum purification conditions were as follows: loading concentration of samples with 78.05 mg/mL, flow rate of 2 mL/min, and de-adsorption with 80% ethanol solution. The recovery rate was 88.75% and the relative content achieved 61.58% after purification by XDA-1 resin. The Freundlich model can be used to describe the entirety of the exothermic and physical adsorption processes. In summary, the conclusion which can be made from this research is that the wastewater generated from the debitterizing of apricot kernels can be well treated by resin to recycle the amygdalin and reduce its pollution to environment.

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46

Wang, Kangjing, Liting Zhao, Ting Li, Qian Wang, Zhongyang Ding, and Weifu Dong. "Selective Immobilization of His-Tagged Enzyme on Ni-Chelated Ion Exchange Resin and Its Application in Protein Purification." International Journal of Molecular Sciences 24, no. 4 (February 15, 2023): 3864. http://dx.doi.org/10.3390/ijms24043864.

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Ion exchange resins are suitable as carriers for immobilized enzymes because of their stable physicochemical properties, appropriate particle size and pore structure, and lower loss in continuous operation. In this paper, we report the application of the Ni-chelated ion exchange resin in the immobilization of His-tagged enzyme and protein purification. Acrylic weak acid cation exchange resin (D113H) was selected from four cationic macroporous resins that could chelate the transition metal ion Ni. The maximum adsorption capacity of Ni was ~198 mg/g. Phosphomannose isomerase (PMI) can be successfully immobilized on Ni-chelated D113H from crude enzyme solution through chelation of transition metal ions with the His-tag on the enzyme. The maximum amount of immobilized PMI on the resin was ~143 mg/g. Notably, the immobilized enzyme showed excellent reusability and maintained 92% of its initial activity with 10 cycles of catalytic reaction. In addition, PMI was successfully purified using an affinity chromatography column prepared by Ni-chelated D113H, which showed the potential for the immobilization and purification process to be realized in one step.

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47

Jiang, Haiyun, Li Kong, Hongguang Tang, Zhenzhen Wang, Caiping Liu, Jianhui Zhang, Yuxin Chen, Jinyang Shen, and Yue Zhou. "Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum." PLOS ONE 19, no. 1 (January 30, 2024): e0297434. http://dx.doi.org/10.1371/journal.pone.0297434.

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This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of SPPE was 7.56 mg GAE/g and the polyphenol purity reached 70.5% after macroporous resin and Sephadex LH-20 purification. A total of 50 compounds were identified by UPLC-QTOF-MS/MS. The IC50 values of SPPE were 334.9 μg/mL, 6.290 μg /mL, 0.834 mg /mL and 0.6538 mg /mL for α-amylase, α-glucosidase, tyrosinase and AchE, respectively. Molecular docking technology further revealed the effects of SPPE on the above enzymes. This study provided information on the potential hypoglycemic, whitening and anti-Alzheimer’s disease biological activities of SPPE, which had guiding significance for the purification and development of other seaweed polyphenols.

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48

Wang, Yuqiong, Xiaoxiao Huang, Yali Sun, Shouqian Zhao, and Yahui Yue. "A new method for the separation of LREEs in geological materials using a single TODGA resin column and its application to the determination of Nd isotope compositions by MC-ICPMS." Analytical Methods 9, no. 23 (2017): 3531–40. http://dx.doi.org/10.1039/c7ay00966f.

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49

Roncal, Tomás, Ainhoa Aguirre, Yolanda Belaustegui, and Elisabet Andrés. "Recovery and purification of acetic acid from extremely diluted solutions using a mixed bed ion exchange resin – technical feasibility." RSC Advances 15, no. 1 (2025): 477–88. https://doi.org/10.1039/d4ra08341e.

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Анотація:

A downstream process for the recovery, purification and concentration of acetic acid from an extremely diluted solution, involving demineralization, treatment with a mixed bed ion exchange resin and step-elution with H2SO4.

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50

Jankowska-Anyszka, Marzena, Maciej Nogalski, and Edward Darzynkiewicz. "NEW AFFINITY RESIN FOR PURIFICATION OF CAP-BINDING PROTEINS." Nucleosides, Nucleotides & Nucleic Acids 24, no. 5-7 (April 1, 2005): 503–6. http://dx.doi.org/10.1081/ncn-200061782.

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