Дисертації з теми "Replication of virus"
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Thomas, C. M. "Cauliflower mosaic virus DNA replication." Thesis, Bucks New University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374828.
Повний текст джерелаEkström, Jens-Ola. "Ljungan Virus Replication in Cell Culture." Doctoral thesis, Högskolan i Kalmar, Naturvetenskapliga institutionen, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.
Повний текст джерелаEkström, Jens-Ola. "Ljungan virus replication in cell culture /." Kalmar : University of Kalmar, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.
Повний текст джерелаMcQuillin, Andrew. "Aspects of cucumber mosaic virus replication." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321682.
Повний текст джерелаEvans, Elizabeth Van Amburg. "Molecular genetic analysis of a vaccinia virus gene with an essential role in DNA replication /." Access full-text from WCMC, 1989. http://proquest.umi.com/pqdweb?did=744576211&sid=1&Fmt=2&clientId=8424&RQT=309&VName=PQD.
Повний текст джерелаNayak, Arabinda. "Foot and mouth disease virus RNA replication." Thesis, University of Surrey, 2005. http://epubs.surrey.ac.uk/842873/.
Повний текст джерелаSzemiel, Agnieszka M. "Replication of Bunyamwera virus in mosquito cells." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/2570.
Повний текст джерелаNapoli, Andrea. "Glycerophospholipid fluorescence imaging during vaccinia virus replication." Thesis, Sorbonne Paris Cité, 2019. https://theses.md.univ-paris-diderot.fr/NAPOLI_Andrea_1_va_20190415.pdf.
Повний текст джерелаVaccinia Virus (VACV) is the model organism for the study of the Poxviridae. Its cytoplasmic life cycle has been studied extensively by light- and electron microscopy. Thanks to a robust genetic system the role of some of its 250 proteins is beginning to be understood. Nevertheless, the acquisition of its membranes is still a matter of debate, in particular the role of cellular lipids. Lipid mass spectrometry of purified VACV previously showed an enrichment of phosphatidic acid (PA) and phosphatidylinositol derivatives (PIPs) in the viral membrane. Although some viral proteins have been shown to bind PIPs in vitro the role of these lipids in the viral life cycle, in particular viral membrane biogenesis, remains elusive.The aim of this work is to determine whether PA and PIPs are relocated in infected cells to the site of viral membrane biogenesis. For both PA and PIPs, I used recombinant proteins containing PA or PIP binding domains fused to eGFP, expressed them by transient transfection to follow their localization during viral replication. In addition, I used antibodies for the recognition of PI4K and PI4P. In order to understand the biochemical role of PIPs, I used pan-PI3K and PI4K inhibitors to study their effect on viral assembly. Using these tools, I could show that the lipids under investigation did not display an altered localization, with the exception of PI3P which showed a different pattern in infected cells. None of the PIPs analyzed co-localized with the sites of primary VACV membrane biogenesis. Consistent with the fact that the mature virus acquires additional membranes derived from the Golgi complex, I could show a co-localization of wrapped virus with PI4P, known to localize to this cellular organelle. However, drugs inhibiting PI3P and PI4P biosynthesis did not show any effect on VACV membrane biogenesis, at least at the light microscopy level. In conclusion, this work sharper defines the role of lipids during VACV replication. In particular, it opens the way to further studies on the putative role of PI4P during wrapping and the fate of PI3P and PI3P binding proteins during late replication
Lu, Jia. "Norovirus translation and replication." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/278610.
Повний текст джерелаXing, Xuekun. "DNA replication and telomere resolution in vaccinia virus." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq23557.pdf.
Повний текст джерелаBao, Yiming. "The DNA replication of rice tungro bacilliform virus." Thesis, University of East Anglia, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386295.
Повний текст джерелаFrampton, Nicholas Ross. "Impact of hypoxia on hepatitis B virus replication." Thesis, University of Birmingham, 2018. http://etheses.bham.ac.uk//id/eprint/8467/.
Повний текст джерелаNilsson, Benjamin Erik. "Viral and host factors regulating influenza virus replication." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:b8953952-e6d5-4f6d-a7ba-cd55277611d1.
Повний текст джерелаPina, Mery. "Replication of extrachromosomal elements of hyperthermophilic archaea." Paris 6, 2011. http://www.theses.fr/2011PA066557.
Повний текст джерелаReplication of extrachromosomal elements of the Crenarchaeota remains to be poorly understood. More than 96% of viral ORFs do not have homologs in public databases, and thus their functions cannot be predicted based on sequence similarity. The aim of the present study was to understand the mechanisms of replication of extrachromosomal elements in Crenarchaeota in the frame of the genome of the Acidianus filamentous virus 1 (AFV1) of Acidianus hospitalis, and of the plasmid pRN1 of Sulfolobus islandicus. By applying in silico genome sequence analysis, two dimensional agarose gel electrophoresis (2DAGE) and dark field electron microscopy we postulated that the replication mechanism exploited by AFV1 is recombination-dependent replication. A terminal protein was found to be strongly attached to the 5’- ends of the encapsidated viral genome. Tertiary structures were promoted by the interaction of this protein with internal regions of the genome. Characterization of the replicative intermediates of pRN1 was performed using 2DAGE. The existence of intermediates of the same size, but different secondary structure supported the hypothesis of a stem-loop that was experimentally found to be inside the minimal replicon of the plasmid. A replication origin was found in the 2DAGE at this same site. The biochemical characterization of AFV1-ORF157 is also included in this dissertation. A nuclease activity on linear double-stranded DNA was shown to be present in ORF-157, giving rise to the hypothesis that this protein might be involved in edition of mature genomes for encapsidation
Kandpal, Manish. "Role of defective hepatitis B virus in wild-type hepatitis B virus replication." Thesis, IIT Delhi, 2017. http://localhost:8080/xmlui/handle/12345678/7247.
Повний текст джерелаPenzes, Zoltan. "Defective replicating RNAs of coronavirus infectious bronchitis virus : investigation of replication and genome packaging signals." Thesis, University of Hertfordshire, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283879.
Повний текст джерелаRozen-Gagnon, Kathryn. "Chikungunya virus nonstructural proteins regulate replication fidelity and pathogenicity in vivo." Paris 7, 2014. http://www.theses.fr/2014PA077199.
Повний текст джерелаArboviruses cycle through both vertebrates and invertebrates, which requires them to adapt to disparate hosts while maintaining genetic integrity during genome replication. To study the genetic mechanisms and determinants of these processes, we use chikungunya virus (CHIKV), a re-emerging human pathogen transmitted by the Aedes mosquito. We isolated novel mutators with decreased replication fidelity and higher mutation frequencies, allowing us to examine the fitness of error-prone arbovirus variants. Although CHIKV mutators displayed no major replication defects in mammalian cell culture, they were attenuated in vivo. Unexpectedly, mutator phenotypes were suppressed in mosquito cells and the variants exhibited significant defects in RNA synthesis. Consequently, these replication defects resulted in strong selection for reversion during inection of mosquitoes. Since residue 483 is conserved among alphaviruses, we examined the analogous mutations in Sindbis virus (SINV), which also reduced polymerase fidelity and generated replication defects in mosquito cells. However, replication defects were mosquito cell-specific and were not observed in Drosophila S2 cells, allowing us to evaluate the potential attenuation of mutators in insect models where pressure for reversion was absent. Indeed, the SINV mutator variant was attenuated in fruit flies. These findings confirm that residue 483 is a determinant regulating alphavirus polymerase fidelity and demonstrate proof of principle that arboviruses can be attenuated in mammalian and insect hosts by reducing fidelity
Fodor, Ervin. "Studies on the vRNA promoter of influenza A virus." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294232.
Повний текст джерелаKabdulov, Timur O. "Mechanisms of retroviral replication." Morgantown, W. Va. : [West Virginia University Libraries], 2001. https://etd.wvu.edu/etd/controller.jsp?moduleName=documentdata&jsp%5FetdId=2256.
Повний текст джерелаMolin, Ylva. "Arsenic Influences Virus Replication in Experimental Coxsackievirus B3 Infection." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-112049.
Повний текст джерелаBasak, Sanjukta. "Studies of Hepatitis C virus immunology : translation and replication." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97903.
Повний текст джерелаRecent efforts to produce efficient vaccines require not only the identification of potential viral antigens but also vaccine adjuvants or enhancers of immunity. Dendritic cells (DC) are being considered one such adjuvant for the activation of CD4+ and CD8+ T-cells. As potent antigen presenting cells, they are capable of capturing antigens, processing them into peptides, and presenting them on products of the MHC to T cells. For such reasons, peptide loading of antigens onto DCs to enhance T cell responses is becoming of increasing interest. Using cell penetrating peptides, or motifs capable of transporting cargo freely across cell membranes, we have developed a peptide based delivery system suitable for the transport of all HCV proteins into immature DCs. In our studies we demonstrated that 3.1% of immature DCs internalized the reporter cargo, eGFP. This system was then optimized to 53.81 % in target HeLa cells.
Another area of recent focus is the regulation of HCV translation and replication. Positive stranded viruses such as HCV use the genomic RNA as a common template for translation as well as for RNA replication, both proceeding in inverse directions. Thus, specific regulatory mechanisms must be in place in order to coordinate these two antagonistic processes. In this study, we investigated the role of HCV Core protein as a translational inhibitor and enhancer of replication. Using several transient and stable in vivo reporter assays, we showed that Core expression inhibited HCV IRES-mediated translation in trans, in a dose-dependent manner. Furthermore, HCV Core protein is able to dramatically inhibit HCV translation in the Huh7 replicon system, more so than the bicistronic reporter systems tested and subsequently increase total levels of replicon RNA by 1.5 log fold and thus, affect replication. We believe that Core may indeed be the sought regulator of translation and replication.
Cotton, Sophie. "Characterization and movement of turnip mosaic virus replication complexes." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=86558.
Повний текст джерелаThe biogenesis of viral vesicles was investigated by infecting cells with two recombinant TuMVs producing 6KGFP or 6KmCherry-labelled vesicles. Individual vesicle within a cell contained unique protein products derived from each recombinant demonstrating the origin of a vesicle from a single viral genome. Green and red sectoring was also observed, meaning that vesicles could fuse together. The presence of the eukaryotic translation factors eIF(iso)4E, PABP and eEF1A enclosed in VRC was demonstrated previously by our group. These data combined to a single-genome origin suggest that viral translation occur within these structures. The same host factors were also found to co-localize with the active replicating sites along with viral proteins VPg-Pro, RdRp and CI using immunofluorescence labelling in infected protoplasts. These data bring accumulating evidence for the possible coupling of viral translation and replication.
Les virus sont des parasites intracellulaires qui utilisent la cellule hôte pour produire une nouvelle descendance infectieuse. Pour tous les virus à ARN positif étudiés jusqu'à maintenant, la réplication virale prend place dans des structures membranaires induites par le virus. Chez le virus de la mosaïque du navet (TuMV), la formation de vésicules probablement associées au complexe de réplication dépend de la synthèse de la protéine virale 6K. Afin de caractériser les vésicules formées durant l'infection de TuMV, des plants de Nicotiana benthamiana ont été agroinfiltrés avec un clone infectieux de TuMV exprimant la protéine 6K fusionnée à GFP. Des agrégats cytoplasmiques ont été observés par microscopie confocale, correspondant aux vésicules induites par la protéine 6KGFP et abritant le complexe de réplication virale (VRC). Le mouvement intracellulaire de ces vésicules a été visualisé par imagerie time-lapse. Le trafic des vésicules a été inhibé lorsque les plantes étaient infiltrées avec de la latrunculin B, un inhibiteur de polymérisation des microfilaments. L'absence de mouvement a également conduit à une sévère diminution de l'accumulation virale. Les vésicules colocalisent avec les filaments d'actine. Ces résultats indiquent que les microfilaments sont nécessaires au mouvement des vésicules lequel est important pour l'infection virale.
La biogenèse des vésicules virales a été investiguée en infectant les cellules avec deux clones infectieux de TuMV produisant des vésicules étiquetées par 6KGFP ou 6KmCherry. Des vésicules individuelles contenant des protéines uniques dérivant d'un seul clone recombinant a démontré que l'origine des vésicules provient d'un seul génome. Des vésicules ayant des secteurs vert et rouge ont aussi été observé, indiquant que la fusion de vésicules est possible. La présence des facteurs eucaryotes de traduction eIF(iso)4E, PABP et eEF1A à l'intérieur des vésicules a été démontré par notre groupe. Ces données combinées à l'origine unique des vésicules suggèrent que la traduction virale se produit à l'intérieur de ces vésicules. Les mêmes facteurs de traduction ainsi que les protéines virales VPg-Pro, RdRp et CI colocalisent avec les sites de réplication active dans des protoplastes infectés. Ces données apportent des indices supplémentaires sur la possibilité de couplage entre la traduction et la réplication virale chez TuMV.
Stirling, Julie M. "Studies on the replication and assembly of bluetongue virus." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.362312.
Повний текст джерелаMioulet, Valerie. "Analysis of transcription and replication signals in rinderpest virus." Thesis, University of Hertfordshire, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323458.
Повний текст джерелаPatel, Hershna. "Evolutionary targeted discovery of influenza A virus replication inhibitors." Thesis, University of Hertfordshire, 2017. http://hdl.handle.net/2299/19623.
Повний текст джерелаBerthold, François. "Grapevine fanleaf virus replication : viral proteins and host factors." Strasbourg, 2015. http://www.theses.fr/2015STRAJ086.
Повний текст джерелаStoner, Terri Dorene. "Indole-3-Carbinol Inhibition of Herpes Simplex Virus Replication." Kent State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=kent1228328838.
Повний текст джерелаBaillie, Andrew James. "Cellular and viral determinants for hepatitis C virus replication." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/6865.
Повний текст джерелаRosskopf, John J. "CIS-acting signals for replication of Nodamura virus RNA1." To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2009. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.
Повний текст джерелаClark, Hayley. "The effect of iron on hepatitis C virus replication." Thesis, Clark, Hayley (2011) The effect of iron on hepatitis C virus replication. Honours thesis, Murdoch University, 2011. https://researchrepository.murdoch.edu.au/id/eprint/7179/.
Повний текст джерелаPiccininni, Sabina. "Structure and function of Hepatitis C virus replication complex." Thesis, University of Manchester, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.488274.
Повний текст джерелаHafner, Gregory. "Replication of banana bunchy top virus : mechanisms and interference." Thesis, Queensland University of Technology, 1998.
Знайти повний текст джерелаDirks, Clarissa A. "The role of cellular factors in retrovirus replication /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/5072.
Повний текст джерела駱淑芳 and Suk-fong Anna Lok. "Replication of hepatitis B virus in Chinese patients with chronic hepatitis B virus infection." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1990. http://hub.hku.hk/bib/B31981392.
Повний текст джерелаBrett, Katharina. "Molecular requirements of influenza virus hemagglutinin for site-specific S-acylation and virus replication." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17274.
Повний текст джерелаInfluenza virus’s hemagglutinin (HA) is post-translationally modified by S-acylation of three cysteines. Two are located in its cytoplasmic tail (CT) and contain palmitate and one at the end of the transmembrane region (TMR) is acylated primarily with stearate. It is hypothesized that either the acylation site’s amino acid environment or its location relative to the membrane determines which type of fatty acid is attached. Additionally, these acylation sites are essential for virus replication. Whether other amino acids in the CT are required for virus replication, is not known. Based on a comprehensive sequence comparison to identify conserved amino acids, recombinant viruses with amino acid substitutions in the vicinity of HA’s acylation sites were created. These substitutions included point mutations, shifting of a TMR cysteine to the CT and the deletion of the entire tail. The truncated tail mutation and a substitution adjacent to an acylated cysteine disabled virus replication. In contrast, a conservative substitution at this position, other exchanges in TMR and CT and moving the TMR cysteine to the CT had only subtle effects on virus growth. Yet, some of the mutated codons reverted to the original or other amino acids. Recombinant viruses were propagated in MDCK cells and embryonated chicken eggs and analyzed by mass spectrometry. No under-acylated peptides were detected, even the two lethal mutations did not abolish acylation. Point mutations only moderately affected the stearate content, while relocating the TMR cysteine to the CT virtually eliminated attachment of stearate. More stearate was attached if human viruses were grown in mammalian compared to avian cells. Hence, the location of an acylation site relative to the TMR represents the principal signal for stearate attachment, while the sequence context and the cell type modulate the fatty acid pattern.
Roy, Dominic. "Improving the Delivery and Replication of Oncolytic Viruses." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/35227.
Повний текст джерелаNajmabadi, Hossein. "Characterization of the Self-Replicating Kirsten Murine Leukemia Viral DNA: Replication and Tetracycline Resistance." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc798479/.
Повний текст джерелаMAENO, KOICHIRO. "Replication of Influenza B Virus: Biological Functions of Viral Neuraminidase." Nagoya University School of Medicine, 1994. http://hdl.handle.net/2237/15935.
Повний текст джерелаMorgan, Rachel E. "Is the Cytoskeleton Necessary for Viral Replication?" Digital Archive @ GSU, 2012. http://digitalarchive.gsu.edu/biology_theses/38.
Повний текст джерелаau, M. Stewart@murdoch edu, and Meredith Stewart. "An investigation into aspects of the replication of Jembrana disease virus." Murdoch University, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20051222.104106.
Повний текст джерелаHuang, Jianhe. "Molecular dissection of the Spodoptera littoralis nucleopolyhedrovirus : virus-host cell interaction and virus DNA replication." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0008/NQ52762.pdf.
Повний текст джерелаHorscroft, Nigel John. "Orbivirus non-structural protein NS2 : its role in virus replication." Thesis, University of Oxford, 1997. http://ora.ox.ac.uk/objects/uuid:9b550db6-dd9d-4127-941f-93eab2b6e038.
Повний текст джерелаGowans, E. J. "Studies on markers of hepatitis B virus replication in man /." Title page, contents and summary only, 1986. http://web4.library.adelaide.edu.au/theses/09PH/09phg722.pdf.
Повний текст джерелаShaw, Andrew Edward. "The Role of Non-Structural Proteins in Bluetongue Virus Replication." Thesis, University College London (University of London), 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.519459.
Повний текст джерелаEscaler, Margarita. "Changes in host gene expression associated with plant virus replication." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302215.
Повний текст джерелаHofer, Julie M. I. "Regulation of gene expression and replication in wheat dwarf virus." Thesis, University of East Anglia, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334331.
Повний текст джерелаDesmond, Elizabeth. "The interference of influenza virus replication by subgenomic ribonucleic acid." Thesis, University of Reading, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339508.
Повний текст джерелаAbdulsattar, Ban Oday. "Coronavirus proteins and their directed evolution to inhibit virus replication." Thesis, University of Reading, 2017. http://centaur.reading.ac.uk/73736/.
Повний текст джерелаDewar, Rebecca Amy. "Targeting cellular nuclear export to inhibit influenza A virus replication." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31349.
Повний текст джерелаWillment, Janet Anne. "Investigations of the molecular determinants of maize streak virus replication." Doctoral thesis, University of Cape Town, 1999. http://hdl.handle.net/11427/9869.
Повний текст джерелаGeminiviruses replicate via a rolling circle mechanism, which initiates at the origin of replication located within the long intergenic region (LIR). The viral replication associated-protein (Rep) in conjunction with the host's DNA replication machinery is responsible for the initiation and termination of the replication cycle from a stem-loop structure, located within the LIR and conserved throughout the three genera of Geminiviridae. The specific interaction between the Rep protein with sequences within the intergenic region has been well characterised for the begomoviruses and to some extent the curtoviruses; however, this interaction in the mastreviruses, and in particular maize streak virus (MSV), has yet to be fully explored. A theoretical model has been proposed based on sequence data and informed by the current understanding of replication specificity in begomoviruses. Due to the lack of conservation of the stem sequence of the stem-loop structure amongst mastreviruses, the model implicates a pair of nucleotide sequence repeats called iterons. These are located within the stem structure, and on the complementary sense side of the LIR. The former is the putative site of Rep interaction with the LIR. These iterons would therefore potentially act as the determinants of replication specificity amongst mastreviruses.