Дисертації з теми "Regenerative engineering"
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Tan, Richard Philip. "Developing Translational Tissue Engineering Solutions for Regenerative Medicine." Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/20200.
Повний текст джерелаGeorge, Julian H. S. "Engineering of fibrous scaffolds for use in regenerative medicine." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/5298.
Повний текст джерелаGebhardt, Matthew. "Evaluation of tissue engineering scaffolds for regenerative endodontic treatment." Thesis, NSUWorks, 2008. https://nsuworks.nova.edu/hpd_cdm_stuetd/10.
Повний текст джерелаSpeccher, Alessandra. "Tissue engineering approaches for brain injury applications." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/262798.
Повний текст джерелаSpeccher, Alessandra. "Tissue engineering approaches for brain injury applications." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/262798.
Повний текст джерелаChong, Cassandra. "Improving 3D Scaffolds for Skin Tissue Engineering using Advanced Biotechnology." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16551.
Повний текст джерелаSharma, Aman. "Regenerative and biomimetic strategies in spinal surgery." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:5eb692c3-1cad-43ff-aeb4-ff74382ee976.
Повний текст джерелаMUSCOLINO, Emanuela. "Polysaccharide hydrogels for regenerative medicine applications." Doctoral thesis, Università degli Studi di Palermo, 2022. http://hdl.handle.net/10447/535885.
Повний текст джерелаPrajaneh, Saengsome. "Effect of cellular positional identity on bone regenerative capacity for tissue engineering." Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/effect-of-cellular-positional-identity-on-bone-regenerative-capacity-for-tissue-engineering(270579b0-278b-4a3d-9f4a-721f4d38e76e).html.
Повний текст джерелаUeda, Yuichiro. "Application of Tissue Engineering with Xenogenic Cells and Tissues for Regenerative Medicine." 京都大学 (Kyoto University), 2004. http://hdl.handle.net/2433/147657.
Повний текст джерелаFink, Jason J. "Aeromobile regenerative supercirculation test stand (ARSTS)." Ohio : Ohio University, 2004. http://www.ohiolink.edu/etd/view.cgi?ohiou1176147288.
Повний текст джерелаSubbarao, Sanjay. "An 8-PSK super regenerative receiver| Design and simulation." Thesis, California State University, Long Beach, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=1596992.
Повний текст джерелаThe Super- Regenerative Reception concept was developed in the early 1900s. The evolution of Heterodyne Reception has forced it into oblivion. However, recent research and development has emphasized its use in short distance and low power wireless applications. The results indicate extremely low power and high gain with small circuit area.
The study herein focuses on a receiver using a Super Regenerative Oscillator to decrypt 8-PSK Signals. Demodulation without the use of a Local Oscillator, a Phase Locked Loop or an Analog to Digital Converter is proposed. The architecture of encoding is presented which is in turn used to generate the necessary input at the receiver. The receiver involves a combined Low Noise Amplifier Super- Regenerative Oscillator architecture in a single stack configuration for the feature of current reuse. Simulation results are presented which confirm the theory. Finally, a comparative study is presented for various modulation techniques including Quadrature Phase Shift Keying and 8-Phase Shift Keying.
Collier, Ian M. "Regenerative braking on bicycles to power LED safety flashers." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/32868.
Повний текст джерелаIncludes bibliographical references (leaves 28-29).
This work develops a method for capturing some of the kinetic energy ordinarily lost during braking on bicycles to power LED safety flashers. The system is designed to eliminate: (a) battery changing in popular LED flashers, and (b) the "generator drag" associated with battery-less human-powered bicycle lights and flashers. System sizing, mechanical design considerations, potential end-user factors, and a model for braking frequencies in urban settings are discussed. With the urban commuter cyclist in mind as a potential user of the regenerative braking system, custom direct-pull brake calipers (or "V-Brakes") were designed and manufactured to include both conventional friction pads in addition to a DC motor to be used as a generator for kinetic energy capture. The energy captured by the DC motor during braking is passed through a full wave bridge to a bank of Nickel-Cadmium batteries at an efficiency of 79%. The output of the full wave bridge and the batteries are connected in parallel with a step-down switching voltage regulator, which insulates the LED safety flasher from voltage spikes due to braking at high cycling speeds. The performance of the final prototype was evaluated at cycling speeds ranging from 8 to 19 mph and braking frequencies ranging from 2 to 8 operations/stops per mile of travel.
(cont.) From the mean power flow (charging) into the batteries per unit distance of travel and the power required by LED safety flashers, the effectiveness of the system at each speed and stopping frequency is examined. For cyclists traveling at average speeds of 10 mph or higher, the LED safety flashers can be powered continuously for stopping frequencies of 8 times per mile and semi-continuously (> 50% of the time) for stopping frequencies of at least 4 times per mile. As such, the system is determined to be potentially useful to urban commuter cyclists, who frequently perform braking operations at regularly spaced intersections and traffic signals, and who regularly travel by bicycle in low-light conditions (dawn or dusk), though usually less than 50% of the time.
by Ian M. Collier.
S.B.
Sharp, Duncan McNeill Craig. "Bioactive scaffolds for potential bone regenerative medical applications." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/9520.
Повний текст джерелаJohnson, Nigel Christopher. "All-optical regenerative memory using a single device." Thesis, Aston University, 2009. http://publications.aston.ac.uk/15331/.
Повний текст джерелаNewman, Peter Lionel Harry. "Carbon Nanotubes for Bone Tissue Engineering." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16308.
Повний текст джерелаCanseco, José Antoni. "Tissue engineering the anterior cruciate ligament : a regenerative medicine approach in orthopaedic surgery." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/83965.
Повний текст джерелаVita. Cataloged from PDF version of thesis.
Includes bibliographical references (pages 85-97).
Anterior cruciate ligament (ACL) injuries affect over 200,000 Americans yearly, and many occur in young athletes. Current treatment options include tendon autografts and cadaveric allografts. However, these approaches often lead to secondary medical problems, such as donor-site morbidity and immune rejection. Furthermore, in younger patients these grafts fail to grow, leading to additional complications and underlining the need for the development of new approaches that improve the healing and repair of ligaments and tendons. This thesis aims to develop a technique to engineer ACL from autologous mesenchymal stem cells (MSC) and primary ACL fibroblasts using the basic principles of Tissue Engineering. The first part of the thesis characterizes MSCs isolated from tibial bone marrow as an alternative to hip-derived marrow aspirates. The proximity of the tibia to the surgical site of ACL reconstructions makes it a viable source of marrow derived-MSCs for ligament repair, with less stress for the patient and increased flexibility in the operating room. Characterization was performed by fluorescenceactivated cell sorting for MSC-surface markers, and assays to differentiate MSCs towards adipogenic, osteogenic and chondrogenic lineages. The second part of the thesis describes the effects of in vitro co-cultures of ACL fibroblast and MSC on the expression of ligament-associated markers. The goal was to optimize the cell-cell ratio in order to maximize the positive effects of co-cultures on ligament regeneration. Co-cultures of ACL fibroblasts and MSCs were studied for 14 and 28 days in vitro, and the effects assessed with quantitative mRNA expression and immunofluorescence of ligament markers Collagen type I, Collagen type III and Tenascin-C. Finally, based on the enhancing effect observed in co-cultures, the thesis explores a method to regenerate ACL using a three-dimensional polyglyconate scaffold seeded with cell-hydrogel suspensions containing ACL fibroblasts and MSCs. Constructs were analyzed biochemically and by immunofluorescence after 4 weeks in culture with and without mechanical stimulation. Together, our results establish an experimental framework from which a new technique for ACL repair can be developed. The ultimate goal is to foster the design of a one-stage surgical procedure for improved primary ACL augmentation repair that can soon be translated into clinical practice.
by José Antonio Canseco.
Ph.D.in Biomedical Engineering
Hayes, Samantha. "Biomimetic design and engineering to enhance resilience and regenerative performance outcomes for infrastructure." Thesis, Griffith University, 2020. http://hdl.handle.net/10072/400560.
Повний текст джерелаThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Eng & Built Env
Science, Environment, Engineering and Technology
Full Text
Rutledge, Matthew S. "Aircraft Gearbox Dynamics Subject to Electromechanical Actuator Regenerative Energy Flow." Wright State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=wright1292358176.
Повний текст джерелаChurm, James. "Design and fabrication of a next generation regenerative neural interface." Thesis, University of Birmingham, 2018. http://etheses.bham.ac.uk//id/eprint/8198/.
Повний текст джерелаWarren, David S. "The design of a deep space transponder regenerative ranging unit." Thesis, Massachusetts Institute of Technology, 1995. http://hdl.handle.net/1721.1/35026.
Повний текст джерелаBarto, Taylor. "Design and Control of Electronic Motor Drives for Regenerative Robotics." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu150396313057516.
Повний текст джерелаZhao, Chao. "Optimal transceiver design for non-regenerative MIMO relay systems." Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=121383.
Повний текст джерелаLe relayage multi-entrées multi-sorties (MIMO) permet d'accroître la capacité des systèmes sans fil plus efficacement que sa contrepartie n'utilisant qu'une seule antenne. Les stratégies non-régénératives, dans lesquelles les relais appliquent des matrices de transformation linéaire à leur signaux d'entrée avant de les retransmettre, sont préférées dans de nombre d'applications. La conception de transcepteurs est cruciale afin de pleinement exploiter le grand potentiel qu'offrent les relais MIMO dans les systèmes de communications sans fil. Nous explorons ce problème général à partir de deux perspectives différentes: la combinaison cohérente et l'adaptation. Dans la première perspective, nous concevons des architectures de transcepteur pour un système de type source-simple–relais-multiples–destination-simple (1S-MR-1D) dans lequel la source envoie de l'information à la destination par le biais de plusieurs stations relais en parallèle, de telle sorte que les signaux en provenance des relais se combinent de manière cohérente à la destination. À cette fin, deux approches sont proposées: un schème reposant sur une structure hybride à complexité réduite et une approche d'optimisation basée sur la minimisation de l'erreur quadratique moyenne (MSE). Dans la première approche, les matrices de transformation MIMO non-régénératives utilisées à chacun des relais sont obtenues en cascadant deux sous-structures. Pour chacune de ces sous-structures, nous introduisons une famille paramétrique uni-dimensionnelle de transformations matricielles. Ce schème hybride permet la classification et la comparaison de toutes les combinaisons possibles de ces sous-structures. Les paramètres de conception peuvent de plus être optimisés. Le schème hybride permet d'atteindre un bon équilibre entre la performance et la complexité. Dans la deuxième approche, les matrices de relayage sont conçues de façon à minimiser la MSE entre les symboles transmis et reçus. On considère séparément deux types de contraintes sur la puissance en transmission des relais : la contrainte dite de somme pondérée et la contrainte par relais. Sous la contrainte de somme pondérée, nous développons des expressions mathématiques explicites pour la solution optimale. Sous la contrainte de puissance par relais, nous proposons un algorithme de balancement qui permet de convertir le problème d'optimisation en un problème équivalent avec contrainte de type somme pondérée. De plus, nous étudions le problème de la conception jointe de l'égalisateur MIMO à la destination et des matrices de relais. Les résultats de simulation démontrent une performance supérieure à celle de méthodes existantes. Dans la deuxième perspective, nous proposons un cadre unifié d'optimisation des transcepteurs adaptatifs pour les réseaux de relais MIMO non-régénératifs. La conception de transmetteur basée sur l'information de l'état du canal (CSI) suppose implicitement que les canaux sans fil demeurent constants durant chaque bloc de transmission. Cela implique que les canaux et les transcepteurs optimaux correspondants évoluent graduellement au passage des blocs. Afin de bénéficier de cette propriété, nous proposons une nouvelle approche d'adaptation inter-bloc basée sur le critère de minimisation de la MSE. Dans cette approche, la solution obtenue du bloc précédent est utilisée comme point de départ dans la recherche d'une solution optimale pour le bloc actuel. Fortuitement, il est possible d'optimiser les matrices de relayage de façon analytique en premier lieu, ce qui facilite grandement l'adaptation des paramètres restants au moyen d'algorithmes itératifs tels que celui de la descente de gradient. De plus, le cadre d'optimisation que nous proposons peut être adapté à des topologies de réseau variées par l'imposition de contraintes structurelles sur le modèle. Les résultats de simulations numériques démontrent que l'adaptation inter-bloc peut conduire à une réduction importante de la complexité numérique.
Tabor, Keith Aaron. "The real-time digital control of a regenerative above-knee prosthesis." Thesis, Massachusetts Institute of Technology, 1988. http://hdl.handle.net/1721.1/14562.
Повний текст джерелаIncludes bibliographical references.
by Keith Aaron Tabor.
M.S.
Shaffer, Robyn Denise. "Complementary Strategies to Promote Mesenchymal Stem Cell Differentiation for Ligament Tissue Engineering." Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/40295.
Повний текст джерелаPh. D.
Shekaran, Asha. "Beta 1 integrins in bone formation during development and engineering integrin-specific hydrogels for enhanced bone healing." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/51720.
Повний текст джерелаJeong, Sangkwon. "Development of the regenerative magnetic refrigerator operating between 4.2K and 1.8K." Thesis, Massachusetts Institute of Technology, 1992. http://hdl.handle.net/1721.1/11697.
Повний текст джерелаRockwood, Danielle N. "Characterization of electrospun polymer fibers for applications in cardiac tissue engineering and regenerative medicine." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 155 p, 2008. http://proquest.umi.com/pqdweb?did=1459913201&sid=1&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Повний текст джерелаAhmed, Shehnaz. "Self-reporting scaffolds for in situ monitoring for regenerative medicine and tissue engineering applications." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/49511/.
Повний текст джерелаDosier, Christopher R. "Bone tissue engineering utilizing adult stem cells in biologically functionalized hydrogels." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/47678.
Повний текст джерелаGerstenhaber, Jonathan Arye. "ELECTROSPINNING ROBOT FOR REGENERATIVE COATING OF IMPLANTS." Diss., Temple University Libraries, 2017. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/480434.
Повний текст джерелаPh.D.
Electrospinning of nanofibrous mats and scaffolds enables generation of scaffolding that is not only highly porous, but also has a structure that essentially mimics the natural basement membrane. As a result, the method has proliferated extensively, and is commonly used for diverse applications such as water filtration or tissue engineering, the latter of which may involve the use of natural or synthetic materials. Common laboratory scale electrospinning setups can be built inexpensively with merely a syringe pump, a high voltage supply, and an aluminum foil target. These systems, however, are limited to flat target surface geometries that span several centimeters. While a scaffold can be cut or folded to conform to a bone or other biological surface, spinning directly onto a surface with significant peaks and troughs results in poor fiber uniformity. Furthermore, if an alteration of fiber properties is preferred, the high voltage setup limits user access and customization of parameters during the spinning period. Finally, control of the electric field is compromised by the proximity of grounded electrical components. As its first aim, this project develops a robotic control system to enable custom coatings of arbitrary surfaces. By augmenting the traditional electrospinning system with a three-dimensional robotic control system, electric field focusing fibers, and additional aerodynamic forces terms ‘electroblowing’, the device can be produced across targets with strong topographic anisotropy. The second aim continues to enhance these attributes with biocompatible soy based scaffolds. Craniofacial implants are often complex in geometry, and conformal bandages are particularly hard to produce in these areas. Soy based scaffolds will be produced for 3D-printed replicas of these situations. Finally, the methods developed across this aim enables the development and use of a handheld electrospinning system that combines a coaxial high velocity air flow with the high voltage spinning element to reduce effects of operator error. The final goal of the thesis is to test whether fiber control successfully reduces effects of fiber anisotropy in vitro and to use the enhanced fiber control mechanisms to produce scaffolds with significant anisotropy, depositing aligned fibers at a target point to eventually enable generation of scaffolds with programmable variable spatial alignment similar to tendon. When completed, the systems described will enable custom production of coatings or scaffolds for functionality as scaffolding on medically relevant surfaces. Specifically, this means first, that scaffolds can be used with confidence to improve fixation even of non-cylindrical implants and enhance local tissue integration, and second, that implants can be customized with areas of ‘guidance’ fibers or local drug depots to either promote regeneration and population by surrounding tissue or mimic natural anisotropic cues necessary for mechanical or biological functionality.
Temple University--Theses
Wang, Ruichen. "Modelling, testing and analysis of a regenerative hydraulic shock absorber system." Thesis, University of Huddersfield, 2016. http://eprints.hud.ac.uk/id/eprint/30243/.
Повний текст джерелаFox, Jonathan M. "Cathepsin K Targeting Matrix Regenerative Nanoparticles for Small Abdominal Aortic Aneurysm Repair." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1494333727788713.
Повний текст джерелаBenmerah, Samia. "Design and fabrication of novel regenerative implant based on polymeric material." Thesis, University of Birmingham, 2015. http://etheses.bham.ac.uk//id/eprint/5661/.
Повний текст джерелаSmith, Cynthia Miller. "A Direct-Write Three-Dimensional Bioassembly Tool for Regenerative Medicine." Diss., Tucson, Arizona : University of Arizona, 2005. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu%5Fetd%5F1335%5F1%5Fm.pdf&type=application/pdf.
Повний текст джерелаBalasingham, Karunananthan. "A single-phase fully-regenerative converter for chopper controlled DC drives /." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59821.
Повний текст джерелаIn the proposed scheme the energy produced during regeneration is re-injected into the ac mains supply. The scheme consists of adding to each diode of the front end rectifier a transistor capable of conducting the reverse current, and suppressing the dc link capacitor.
The performance of the complete converter with a single phase ac mains is analysed in terms of operation in both motoring and braking modes. The advantages of the scheme are presented and its characteristics are compared to the standard chopper configuration and to the conventional phase-controlled converter systems. It is shown that the system exhibits high power factor and efficiency and is very compact.
Jackson, JeShaune D. Jackson. "Bench to Bone: Commercializing a Cellular Therapeutic for Regenerative Medicine." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1521472107740449.
Повний текст джерелаBosch, Canals Begoña María. "A bioengineering approach for corneal endothelial regeneration." Doctoral thesis, Universitat Internacional de Catalunya, 2019. http://hdl.handle.net/10803/667398.
Повний текст джерелаCogswell, Frederick James. "Cycle control of a regenerative magnetic refrigerator operating from 4.2 to 15 K." Thesis, Massachusetts Institute of Technology, 1989. http://hdl.handle.net/1721.1/14303.
Повний текст джерелаMeng, Linghui. "Polymer Biomaterial Constructs For Regenerative Medicine and Functional Biological Systems." Case Western Reserve University School of Graduate Studies / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=case1327682278.
Повний текст джерелаVivas, Pradillo Daniel. "Optimisation of a Tissue Engineering Product based on Mesenchymal Stromal Cells aiming to regenerate bony tissue." Doctoral thesis, Universitat de Barcelona, 2018. http://hdl.handle.net/10803/664844.
Повний текст джерелаEl hueso es un tipo de tejido conjuntivo altamente especializado y organizado que proporciona una estructura de soporte rígida y protectora. Además, el hueso es único en su capacidad de autoregeneración sin la formación de una cicatriz fibrótica. A pesar de su potencial regenerador natural, el hueso no es siempre capaz de reparar grandes defectos por sí solo, lo que puede resultar en pérdidas óseas permanentes o en la aparición de pseudoartrosis. Por consiguiente, se requieren intervenciones quirúrgicas para la aplicación de injertos con la finalidad de reemplazar hueso dañado o enfermo. Esto se traduce en la implantación de más de dos millones de injertos óseos anuales en el mundo. Actualmente, los autoinjertos siguen siendo la técnica quirúrgica estándar, pero no están exentos de complicaciones, tales como infecciones o morbilidad asociada a la zona de extracción donante. Las terapias avanzadas (AT), particularmente las aproximaciones dentro de la medicina regenerativa (RM) y la ingeniería de tejidos (TE), ofrecen herramientas valiosas con amplia aplicabilidad en el mundo de la ortopedia con el objetivo de lograr regenerar hueso. Esta tesis doctoral se ha desarrollado dentro del campo de la RM con el objetivo de optimizar la formulación de productos de ingeniería de tejidos (TEPs) compuestos por células mesenquimales estromales (MSCs) con la finalidad de regenerar hueso. Hasta la fecha, se ha acumulado amplia experiencia, tanto preclínica como clínica, demostrando la seguridad e indicios de eficacia de las terapias basadas en el uso de MSCs para diversas indicaciones. Por este motivo, los principales retos en la actualidad se centran en mejorar la eficacia de dichos productos modificando sus formulaciones y prestándole especial atención tanto al tejido de aislamiento de las MSCs como a los componentes no celulares de los TEPs. La propuesta presentada en esta tesis doctoral está basada en MSCs derivadas de médula ósea (BM) o de gelatina de Wharton (WJ) del cordón umbilical (UC) como componente osteogénico, partículas de hueso descelularizadas que aportan las propiedades osteoinductoras y osteoconductoras y un hidrogel de fibrina que confiere la habilidad de adaptarse a la arquitectura de cada defecto en particular. La investigación se ha realizado tanto in vitro como in vivo en un modelo ectópico en ratón (abordado en el CHAPTER IV) y subsecuentemente, en dos modelos ortotópicos en oveja (abordado en el CHAPTER III y en el CHAPTER V) demostrando seguridad y signos de eficacia. La nueva formulación de grado clínico basada en MSCs derivadas de BM resultó ser factible, eficaz y eficiente adaptándose a la arquitectura de los defectos cilíndricos simulados. Por otro lado, este trabajo es un hito en el desarrollo no clínico de los TEPs basados en MSCs derivadas de WJ antes de su aplicación en pacientes. No obstante, se requieren más estudios con el objetivo de desencadenar la rápida diferenciación hacia linaje osteogénico de las MSCs derivadas de WJ en indicaciones específicas de regeneración ósea. Además, los resultados relacionados con la formulación de hueso inyectable la convierten en una alternativa atractiva para ser considerada en futuras aproximaciones de TE relacionadas con la bioimpresión en tres dimensiones (3D), como una potencial biotinta basada en MSC.
Yu, Jiayi. "Tunable Biodegradable Polymers for Regenerative Medicine." University of Akron / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1524821159786707.
Повний текст джерелаGersbach, Charles Alan. "Runx2-Genetically Engineered Skeletal Myoblasts for Bone Tissue Engineering." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/11600.
Повний текст джерелаNieman, Joshua E. "A Novel, Elastically-Based, Regenerative Brake and Launch Assist Mechanism." University of Dayton / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1399048279.
Повний текст джерелаChartrain, Nicholas. "Designing Scaffolds for Directed Cell Response in Tissue Engineering Scaffolds Fabricated by Vat Photopolymerization." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/95939.
Повний текст джерелаDoctor of Philosophy
Vat photopolymerization (VP) is a 3D printing (or additive manufacturing) technology that is capable of fabricating parts with complex geometries with very high resolution. These features make VP an attractive option for the fabrication of scaffolds that have applications in tissue engineering. However, there are few printable materials that are biocompatible and allow cells attachment. In addition, those that have been reported cannot be obtained commercially and their synthesis requires substantial resources and expertise. A novel resin composition formulated from commercially available components was developed, characterized, and printed. Scaffolds were printed with high fidelity. The scaffolds had mechanical properties and water contents that suggested they might be suitable for use in tissue engineering. Fibroblast cells were seeded on the scaffolds and successfully adhered and proliferated on the scaffolds. The growth, migration, and differentiation of cells is influenced by the environmental stimuli they experience. In engineered constructs, the scaffold provides many of stimuli. The geometrical features of scaffolds, including how porous they are, the size and shape of their pores, and their overall size are known to affect cell growth. However, scaffolds that have a variety of pore sizes but identical pore shapes, porosities, and other geometric parameters cannot be fabricated with techniques such as porogen leaching and gas foaming. This has resulted in conflicting reports of optimal pore sizes. In this work, several scaffolds with identical pore shapes and porosities but pore sizes ranging from 200 μm to 600 μm were designed and printed using VP. After seeding with cells, scaffolds with large pores (500-600 μm) had a large number of evenly distributed cells while smaller pores resulted in fewer cells that were unevenly distributed. These results suggest that larger pore sizes are most beneficial for culturing fibroblasts. Multi-material tissue scaffolds were fabricated with VP by selectively photocuring two materials into a single part. The scaffolds, which were printed on an unmodified and commercially available VP system, were seeded with cells. The cells were observed to have attached and grown in much larger numbers in certain regions of the scaffolds which corresponded to regions built from a particular resin. By selectively patterning more than one material in the scaffold, cells could be directed towards certain regions and away from others. The ability to control the location of cells suggests that these printing techniques could be used to organize cells and materials in complex ways reminiscent of native tissue. The organization of these cells might then allow the engineered construct to mimic the function of a native tissue.
Mukhatyar, Vivek. "Understanding the role topographical features play in stimulating the endogenous peripheral nerve regeneration across critically sized nerve gaps." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/45933.
Повний текст джерелаDe, Araújo Júnior José Vitor. "Chitosan/carrageenan-based polyelectrolyte complexes and their composites with calcium phosphate for bone tissue engineering." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608264.
Повний текст джерелаYang, Peter J. "Incorporation of protease-sensitive biomaterial degradation and tensile strain for applications in ligament-bone interface tissue engineering." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/42840.
Повний текст джерелаWung, Nelly. "Tissue engineering of the liver." Thesis, University of Bath, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715264.
Повний текст джерелаCecchin, Denis. "Study and characterization of drug delivery system in regenerative medicine." Doctoral thesis, Università degli studi di Padova, 2010. http://hdl.handle.net/11577/3422729.
Повний текст джерелаRiassunto I sistemi di drug delivery (DDSs) rappresentano una tecnologia particolarmente promettente per migliorare l'efficacia in vivo e in vitro di molecole biologicamente attive con l’obiettivo di circoscriverne l’effetto su una determinata tipologia di cellule, migliorarne l’efficacia, prolungarne il periodo di emivita e ridurre la tossicità di una terapia. In questo lavoro sono stati studiati due modelli di Drug Delivery: il primo riguarda lo sviluppo di nuovi farmaci antitumorali selettivi mediante un coniugato polimerico, mentre il secondo modello, che trova applicazione nell’ambito dell’ingegneria tissutale, riguarda il rilascio controllato di fattori di crescita mediante microsfere. PEG coniugato I problemi più comuni riguardanti i farmaci anti-tumorali possono essere dovuti ad un tempo di emivita basso a causa di clearance renale rapida, all'inattivazione rapida da parte di enzimi, alla scarsa selettività cellulare e spesso ad una scarsa solubilità in ambiente fisiologico, oltre a gravi effetti collaterali. Per cercare di ovviare, almeno in parte, a questi problemi, è stato preparato un coniugato polimerico direzionato al quale è stato legato un farmaco anti-cancro. Il coniugato migliora il profilo farmacocinetico del farmaco riducendo la clearance. Il “selective tumor targeting” può essere attivo o passivo. Il primo riguarda ligandi di recettori associati al tumore, che raggiungono il bersaglio sfruttando l’affinità ligando-recettore. Il secondo sistema può essere ottenuto sfruttando il cosiddetto effetto EPR (enhanced permeability and retention effect) grazie al quale molecole ad alto peso molecolare raggiungono e si accumulano nell’ambiente peritumorale. In questo lavoro è stato utilizzato un poli-(etilenglicole) eterobifunzionale legato ad epirubicina (EPI), un farmaco anti-cancro, e ad acido folico (FOL), come residuo di targeting. L'attività biologica del derivato FOL-PEG-EPI è stata studiata in due diversi sistemi di coltura, il classico sistema bi-dimensionale ed il sistema tri-dimensionale utilizzando Puramatrix hydrogelTM. Quest’ultimo dovrebbe ricreare un ambiente simile a quello in vivo. Gli studi di attività citotossica sono stati effettuati sulle seguenti linee cellulari: HT-29, MCF- 7 e KB-31 che presentano una diversa espressione del recettore di membrana per l’acido folico (rispettivamente normale espressione, medio-alta, alta). Lo studio di citotossicità su FOL-PEG-EPI ha mostrato maggiore tossicità su cellule KB-31, con sovra-espressione del recettore per l’acido folico, rispetto alle cellule MCF-7 e HT-29, sia in colture 2D che 3D. Inoltre, l’utilizzo del sistema di coltura tri-dimensionale ha dimostrato che FOL-PEG-EPI possiede attività selettiva sulle cellule KB-31, rispetto alle cellule HT-29 dove per ottenere l’IC50 è stata utilizzata una concentrazione di coniugato 3 volte più alta della massima utilizzabile in clinica. L’up-take cellulare dei coniugati ed epirubicina sono stati studiati mediante citofluorimetria e microscopia confocale. Nel primo caso, la citofluorimetria ha mostrato la presenza del segnale di fluorescenza all'interno delle cellule sia per FOL-PEG-EPI che per epirubicina. L'analisi di microscopia confocale ha confermato l’internalizzazione, localizzando in zona nucleare il farmaco libero ed in zona perinucleare il coniugato. Microsfere L’ingegneria dei tessuti è un campo interdisciplinare che applica i principi dell’ingegneria e delle scienze della vita allo sviluppo di sostituti biologici per ristabilire, mantenere o migliorare la funzione di tessuti e organi danneggiati. In questa ricerca si fondono discipline di biologia cellulare, ingegneria, scienza dei materiali e chirurgia allo scopo di costruire, mediante la combinazione di cellule, materiali (“scaffold”) e fattori di crescita, nuovi tessuti funzionali. I fattori di crescita possono essere impiegati per riprodurre le condizioni fisiologiche che consentono alle cellule di crescere, moltiplicarsi e differenziarsi nei diversi tipi di tessuti, ma la loro somministrazione rimane ancora una sfida tecnologica a causa della loro breve emivita nonché della loro difficoltà nel raggiungere il sito di targeting. La seconda parte di questo studio ha riguardato lo sviluppo di un sistema di Drug Delivery applicato all’ingegneria tissutale del tessuto osseo. La ricerca ha coinvolto l'utilizzo di un sistema di veicolazione di farmaci per il rilascio controllato della proteina TAT-OP1, che stimola la differenziazione osteogenica. Osteogenic protein-1 (OP-1 o BMP-7) è un membro della famiglia delle proteine morfogeniche dell’osso (bone morphogenic proteins, BMPs). Le BMP vengono riconosciute come fattori di crescita osteoinduttivi, ovvero promotori della formazione di nuovo tessuto osseo e appartengono alla superfamiglia del TGF-β. Le BMP sono secreti come precursori circa quattro volte più lunghi rispetto alla forma matura e possiedono una porzione C-terminale distintiva (pattern .. C ... CXGXC ... CC ... CXCX ..) contenente sette cisteine che costituiscono la regione attiva di queste proteine. In questo studio è stata utilizzata una proteina ricombinante di fusione chiamata TAT-OP1 che comprende una sequenza TAT, un peptide ricco di arginina derivante dall’HIV e che permette l’internalizzazione. Il costrutto TAT-OP1, di 162 aminoacidi, comprende: una porzione N-terminale 6 His-tag seguito dalla sequenza TAT, un sito di cleavage peptidasi-specifico (spanning 6 AA) e il C-terminale con il dominio OP-1 (126 AA) contenente il motivo di cisteine. Quando questo tipo di molecola bioattiva viene iniettato direttamente nel sito di azione, viene sottoposta ad inattivazione e rapida diluizione; questo ne limita l'uso in vivo. Per ovviare al problema sono state impiegate microsfere di poli-lattidecoglicolide (PLGA) per permettere un rilascio controllato di TAT-OP1 con l'obiettivo di mantenere un livello adeguato della proteina per tempi prolungati, migliorandone l’efficienza. Il rilascio delle molecole bioattive può essere facilmente modulato settando i parametri nella formulazione e nella tecnica di produzione. La tecnica dello spray drying è stata utilizzata per ottenere le microsfere con TAT-OP1. Il rilascio dalle microsfere con TAT-OP1 è stato studiato in un periodo di 7 giorni e l'efficienza di incapsulamento era risultata del 35%. Le dimensioni al microscopio a scansione elettronica (SEM) risultavano comprese tra 0,2-2 µm. Lo studio dell’attività biologica su microsfere con TAT- OP1, è stato condotto utilizzando pre-osteoblasti MC3T3-E1 a due diverse concentrazioni, 200 e 27 nM. Dopo 7 e 14 giorni di trattamento, le cellule mostravano presenza di mineralizzazione della matrice, test per la fosfatasi alcalina positivo e presenza di caratteristici marcatori osteogenici, quali osteopontina e osteocalcina. Questi risultati positivi ci hanno portato a valutare l'attività biologica della TAT- OP1 in microsfere in un sistema tri-dimensionale utilizzando cellule staminali mesenchimali isolate dal sangue del cordone ombelicale (UCBMSC). Il modello 3D è stata ottenuto utilizzando la matrice sintetica Puramatrix hydrogelTM, che è in grado di simulare il microambiente fisiologico. A seguito dell’incapsulazione di TAT-OP1 libera o di microsfere con TAT-OP1 in Puramatrix hydrogelTM, la risposta cellulare alla stimolazione di TAT-OP1 è stata valutata grazie all'analisi di microscopia elettronica a trasmissione (TEM) per rilevare la produzione di matrice ossea. Dopo 27 giorni di stimolazione con TAT-OP1 (200 nM), si osservava la presenza di microfibrille parzialmente aggregate attorno alle cellule. Depositi di calcio e cristalli di idrossiapatite sono stati rilevati solo in culture trattate con microsfere a rilascio controllato di TAT-OP1 (200nM). Pertanto, il rilascio controllato di TAT-OP1 da microsfere di PLGA sembra aumentare l’efficacia di stimolazione. Future indagini saranno dirette a confermare ulteriormente la capacità del presente approccio nel migliorare lo studio di differenziamento osteogenico in vitro e l'attività biologica della TAT-OP1 per un eventuale applicazione clinica nel campo dell’ingegneria tissutale dell’osso.