Дисертації з теми "RecR"
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Whitby, Matthew Conway. "Molecular and biochemical analysis of the recR operon of Escherichia coli K-12." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335405.
Повний текст джерелаMahdi, Akeel Abdulla. "Genetic and molecular analysis of the recR locus of Escherichia coli K-12." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315066.
Повний текст джерелаCibele, de Souza Gomes Tatiane. "Desenvolvimento, mecanismo e reversão da resistência ao Temephos na linhagem Aedes aegypti (Diptera: Culicidae) Recife-resistente (RecR)." Universidade Federal de Pernambuco, 2009. https://repositorio.ufpe.br/handle/123456789/1070.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
Brasil, desde 1996, levou ao aparecimento de populações de mosquitos resistentes a esse composto. Apesar disso, o produto continua sendo usado pelo governo, exceto nos locais de detecção da resistência, onde foi substituído por larvicidas biológicos. O conhecimento sobre a forma de desenvolvimento e reversão da resistência em campo, bem como os mecanismos que modulam sua manifestação, pouco avançou nos últimos anos, apesar destas informações serem necessárias para a elaboração de esquemas seguros de manejo da resistência. Este trabalho se propôs a avaliar, utilizando uma linhagem de A. aegypti resistente ao temephos, os mecanismos responsáveis, ao menos em parte, por esta resistência, a possibilidade de respostas cruzadas com outros inseticidas e a reversão à susceptibilidade a este composto, em diferentes situações que simulam a realidade em campo. Assim, diferentes gerações da linhagem de A. aegypti, Recife-Resistente, RecR (14ª e 17ª gerações) mantidas sob forte pressão de seleção ao temephos, foram utilizadas. Como controle, utilizou-se uma linhagem padrão de susceptibilidade, a Rockefeller. Ensaios in vivo com concentrações múltiplas do temephos foram realizados para calcular a CL50 e CL90 e definir a razão de resistência (RR) nas diferentes gerações da RecR. A susceptibilidade da RecR a outros inseticidas, como o regulador de crescimento pyriproxyfen e os adulticidas malathion (organofosforado), deltametrina e cipermetrina (piretróides) foi verificada através de bioensaios dose-resposta (DR) e dosediagnóstica (DD). Para estudos preliminares dos mecanismos que conferem resistência, a atividade de enzimas associadas à detoxificação de inseticidas, como a glutationa S-transferase (GST s), esterases (EST s) α e β e oxidases de função mista (MFO s), também foi analisada na RecR. Para o estudo da reversão da resistência foram estabelecidas três sublinhagens. Duas delas foram provenientes da 14ª geração da RecR (RecRF14), sendo que uma foi mantida sem exposição ao temephos (RecRev1) e a outra sem exposição e com introdução de 30% de indivíduos com baixa resistência (RecRev2). A terceira sublinhagem, proveniente da 17ª geração da RecR (RecRF17), além de não ter sido exposta contou com a introdução de 50% de indivíduos susceptíveis-Rockefeller (RecRev3), a cada nova geração. Os resultados demonstraram que a RecR, apesar de altamente resistente ao temephos, apresentou resposta alterada ao pyriproxyfen e à cipermetrina e susceptibilidade à deltametrina e ao malathion, o que revela a inexistência de resistência cruzada aos dois últimos compostos. Todas as enzimas, em especial as GST s, mostraram atividade alterada nas fases adulta e larvária da RecRF17, exceto as MFO s, portanto é possível sugerir o envolvimento do mecanismo metabólico na resistência ao temephos. Quanto à reversão da resistência, observou-se que cessada a pressão de exposição ao temephos, após nove gerações consecutivas, houve uma redução na RR90 de 14 vezes (8,7) e 42 vezes (3,0) para RecRev1 e RecRev2, respectivamente. A RecRev3 recuperou a susceptibilidade ao composto na F3. Estes resultados demonstraram uma queda drástica na RR nas três condições avaliadas, mas revelam que a resistência ao composto não regride rapidamente diante da simples interrupção de seu uso, como observado na RecRev1, que permaneceu com nível intermediário de resistência (RR= 8,7). Por outro lado, os esquemas que tentaram simular condições de campo relativas à migração de indivíduos susceptíveis ou com baixa resistência mostraram-se mais eficientes na recuperação da susceptibilidade, revelando o caráter instável desta resistência. É possível sugerir, por fim, que a resistência ao composto é reversível e que métodos baseados na liberação de machos susceptíveis possam representar mais uma forma de manejar a resistência ao temephos em campo
Rosdahl, Charlotte, Mathilda Adrian, and Evelina Ketola. "Rekrytering och ledarskapsstrategier inom evenemangsorganisationer : att rekrytera och leda evenemangets viktigaste resurs." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-22403.
Повний текст джерелаThis study examines the leadership strategies of event organizations in their work with volunteers. Since earlier studies tend to be based solely on volunteers' perspectives, we found it interesting to conduct a study from an organizational perspective, which was made possible by semi-structured interviews with event organizations. The purpose of this thesis is to investigate the leadership strategies that different event organizations applies when recruiting and leading volunteers. Three research questions were asked to answer our purpose: Which strategies do event organizations use when recruiting volunteers? Which strategies do event organizations use when leading volunteers? Which similarities and differences are there between the strategies that the event organizations use when recruiting and leading volunteers? This thesis is using four theoretical frameworks that help us to answer our research questions. Firstly, the framework Volunteer functions inventory, used an index to investigate how strategies are related to volunteer motivation factors. Secondly, the theory of The five practices of exemplary leadership, explaining how leaders will achieve idealistic leadership is presented. Thirdly, a theory for ideal strategy when recruiting volunteers, volunteer recruitment is explained. Lastly, Transformational leadership that describes a leadership style with the aim of involving volunteers in decision-making processes is presented. The study results indicate that event organizations formulates strategies for deciding how volunteers should be recruited and managed and that there are many similarities and differences found between these strategies. It is possible to identify that motivational factors, requirements, goals and visions, involvement and communication are the main factors that event organizations highlight as important to attract and satisfy volunteers. The result should be the basis for how organizations can recruit and lead volunteers through events via their leadership to create the result that event organizations strives for. The study will also help to understand if organizations in the event industry use strategies for how to recruit new volunteers and retain existing volunteers. The language of this thesis is Swedish.
Vickridge, Elise. "Management of E. coli sister chromatid cohesion in response to genotoxic stress." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS172/document.
Повний текст джерелаMaintaining genome integrity through replication is an essential process for the cell cycle. However, many factors can compromise this replication and thus the genome integrity. Mitomycin C is a genotoxic agent that creates a covalent link between the two DNA strands. When the replication fork encounters the DNA crosslink, it breaks and creates a DNA double strand break (DSB). Escherichia coli (E.coli) is a widely used model for studying complex DNA mechanisms. When facing a DNA DSB, E. coli activates the SOS response pathway. The SOS response comprises over 50 genes that are under the control of a LexA-repressed promoter. Upon a DSB induction, RecA, a central protein of the SOS response will trigger the degradation of LexA and all the SOS genes will be expressed.We have developed a novel molecular biology tool that reveals contacts between sister chromatids that are cohesive. It has been shown in the lab (Lesterlin et al. 2012) that during a regular cell cycle, the two newly replicated sister chromatids stay in close contact for 10 to 20 min before segregating to separate cell halves thanks to the action of Topoisomerase IV. This step is called sister chromatid cohesion. We have used this molecular biology tool to study sister chromatid cohesion upon a genotoxic stress induced by mitomycin C (MMC). We have shown that sister chromatid cohesion is maintained and prolonged when the cell is facing a DSB. Moreover, this sister chromatid cohesion is dependent on RecN, an SOS induced structural maintenance of chromosome-like (SMC-like) protein. In the absence of RecN, the proximity between both sister chromatids is lost and this has a deleterious effect on cell viability. By tagging the chromosome with fluorescent proteins, we have revealed that RecN can also mediated a progressive regression of two previously segregated sister chromatids and this is coordinated with a whole nucleoid compaction. Further studies showed that this genome compaction is orderly and is not the result of a random compaction in response to DNA damage.Interestingly, inhibiting TopoIV in a recN mutant fully restores viability and sister chromatid cohesion suggesting that RecN’s action is mainly structural. Preserving cohesion through precatenanes is sufficient to favor repair and cell viability even in the absence of RecN.An RNA-seq experiment in a WT strain and a recN mutant revealed that the whole SOS response is downregulated in a recN mutant. This suggests that RecN may have an effect on the induction of the SOS response and thus RecA filament formation. This is in good agreement with the change in RecA-mcherry foci formation we observed. In the WT strain, the RecA-mcherry foci are defined as described in previous work. However, in the recN, the RecA-mcherry foci seemed to form bundle like structures. These RecA bundles were previsously described by Lesterlin et al. in the particular case of a DSB occurring on a chromatid that has already been segregated from its homolog. This could mean that in the absence of recN, the sister chromatids segregate and RecA forms bundle like structures in order to perform a search for the intact homologous sister chromatid.Altogether, these results reveal that RecN is an essential protein for sister chromatid cohesion upon a genotoxic stress. RecN favors sister chromatid cohesion by preventing their segregation. Through a whole nucleoid rearrangement, RecN mediates sister chromatid regression, favoring DNA repair and cell viability
Taylor, L. "The recB and recC gene products of Escherichia coli." Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355080.
Повний текст джерелаWilson, R. E. "The recB-recC region of the Escherichia coli chromosome." Thesis, University of Newcastle Upon Tyne, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375598.
Повний текст джерелаDulermo, Rémi. "Etude des mécanismes de l'extrême tolérance aux radiations de la bactérie Deinococcus deserti par une approche de génomique fonctionnelle." Aix-Marseille 2, 2009. http://theses.univ-amu.fr.lama.univ-amu.fr/2009AIX22100.pdf.
Повний текст джерелаThe genome of Deinococcus deserti, a highly radiation-tolerant bacterium, was analyzed and compared to those of D. Radiodurans and D. Geothermalis. About 230 proteins are specifically conserved in these 3 species, including IrrE, a regulator protein essential for radiotolerance. D. Deserti has several supplementary DNA repair genes, like imuY and dnaE2 (translesion DNA polymerases). Moreover, D. Deserti has 3 recA that code for 2 different RecA proteins (RecAC et RecAP). To study these genes, genetic tools were developed for D. Deserti. Different results suggest that IrrE, required for the induction of several genes after irradiation, has peptidase activity. The 2 RecA proteins are functional for DNA repair. D. Deserti is mutable by UV, which requires ImuY, DnaE2 and RecAC, but not RecAP
Peters, Helene. "Expressão do Reck, um inibidor de metaloproteinases de matriz, no desenvolvimento pos-natal e na regressão prostatica pos-castração." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317570.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-05T10:11:12Z (GMT). No. of bitstreams: 1 Peters_Helene_M.pdf: 3147205 bytes, checksum: 29d84ddab25f17a17efc857545126663 (MD5) Previous issue date: 2005
Resumo: A próstata tem merecido crescente atenção devido à maior incidência de câncer prostático e outras afecções do órgão, que resultam do aumento na longevidade dos indivíduos do sexo masculino em todo o mundo. Além disto, o desenvolvimento e crescimento prostático normal apresenta regulação androgênica e está sujeito a uma série de disruptores endócrinos que afetam o seu crescimento e função, assim como predispõem ao desenvolvimento tumoral. Nosso interesse reside principalmente na remodelação prostática seguida à castração e nas interações epitélio estroma que ocorrem neste órgão. Neste trabalho, investigamos a expressão do inibidor de metaloproteinases (MMPs) RECK, em nível de RNAm, procurando correlacioná-Io com o desenvolvimento pós-natal e com a regressão prostática seguida à castração. Para isto, foram utilizadas técnicas de RT-PCR semiquantitativo, Real time RT-PCR e de hibridação in situ,pareados sempre que possível com a expressão do RNAm e com a atividade de algumas MMPs. Os resultados demonstram que o gene RECK é expresso na próstata ventral de ratos, que existe uma significativa redução na sua expressão ao longo do desenvolvimento pós-natal, que há mecanismos diferenciados controlando a expressão dos pares RECKlMMP-2 e MMP-7/MMP-14. Foi observado também um crescente aCÚInulo da forma ativa da MMP-9, conforme o animal se aproxima da idade adulta. Utilizando RT-PCR semiquantitativo, pudemos determinar que o conteúdo relativo do RNAm para o RECK após a castração não muda, embora haja uma inversão no balanço entre a expressão epitelial (células epiteliais) e estromal (células musculares lisas e fibroblastos), nesta situação. No conjunto, os resultados sugerem que o RECK é expresso por diferentes tipos celulares da próstata ventral de ratos, com mecanismos de regulação complexos provavelmente oriundos da existência de diferentes compartimentos no órgão, ao contrário do que se observa para células isoladas
Abstract: The prostate has deserved increasingly attention due to the growing incidence of prostatic cancer and other prostatic diseases, which can be related to the longevity increase of men around the world. Besides, the normal prostatic development is under androgen regulation and as so is subject to a series of endocrine disruptors which affect its growth and function and predisposes to prostate cancer. Our interest resides on the prostatic remodelling following castration and on the epithelial-stromal relationships known to occur in the organ. In this work, we have investigated the expression of the matrix metalloproteinase inhibitor RECK, at the rnRNA leveI, trying to correlate its expression with the post natal prostatic development and regression after castration, using semiquantitative RT-PCR, Real time RT-PCR and in situ hybridization, paralleled with the determination of some MMPs expression and activity. Tbe results demonstrate that RECK is expressed in the rat ventral prostate, that there is a significative reduction in its expression during the post natal development, which is paralleled by the expression of some MMPs and that the mechanisms controling the pairs RECKJMMP-2 and MMP-7/MMP-14 are different. It was also observed an increased proportion of the active form of MMP-9, as the animal approaches adulthood. Using semiquantitative RT-PCR, we could determine that the relative content ofRECK rnRNA remains unchanged by castration, spite detecting an inversion in the balance between the epithelial (epithelial cells) and stromal (smooth muscle cells and fibroblasts) in this situation. Taken together, the results indicate that RECK is expressed by different cell types of the rat ventral prostate, with regulatory mechanisms appearing more complex, likely resulting ftom the existence of different compartments in the organ opposing what was seen for isolated cells
Mestrado
Biologia Celular
Mestre em Biologia Celular e Estrutural
Holmberg, Pär, and Jennie Argerich. "Life Cycle Assessment : A Comparison Between a New Produced and a Remanufactured Rear Subframe." Thesis, Uppsala universitet, Institutionen för teknikvetenskaper, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-177282.
Повний текст джерелаRech, Alexander [Verfasser]. "Werkwohnungen / Alexander Rech." Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2016. http://d-nb.info/1102805289/34.
Повний текст джерелаGonzalez, Pablo. "Nominal exchange rate pegging, escape clauses and targeting of the real exchange rate." Texas A&M University, 2003. http://hdl.handle.net/1969.1/3916.
Повний текст джерелаHaga, Raquel Brandão. "Inibição da migração mediada pelo gene RECK em modelo de glioma humano através de alterações no citoesqueleto e adesão focal." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-11092012-134839/.
Повний текст джерелаGliomas are highly invasive, treatment-resistant and lethal tumors. Overexpression of RECK in human glioma cell line T98G decreased cell migration and invasion in vitro, lead to cytoskeleton rearrangement and caused changes in phospho-FAK distribution. However, the pathway involved in RECK-mediated inhibition of cell migration has not been elucidated yet. To study the mechanisms by which RECK affects cell motility, T98G cells were transfected with pCXN2-hRECK vector (RECK+). Some proteins involved in the integrin pathway, activity of some proteins of RhoGTPase family and cytoskeleton proteins were analyzed through immunoblotting, immunostaining and pull-down assay in RECK+ cells and compared with non-transfected T98G cells, T98G transfected with pCXN2 without RECK gene and human primary fibroblasts (FF287). Our results showed an increase in integrin β1 expression and a decrease in FAK phosphorylation in the Tyr397 site, which together with the increase of stress fibers and decrease of lamellipodia, suggest a less migratory phenotype. Despite this, Rac1 activity was increased even though one of Rac activation pathways is through phospho-FAK, leading to lamellipodium formation. Our hypotheses is that RECK affects focal adhesion turnover, diminishing cell motility. As cells are still receiving a positive signal to migrate, they activate Rac1 through a FAK-independent pathway. Besides that, paxillin immunostaining showed that focal adhesions are larger in RECK+ cells, indicating that RECK can influence structures related with cell-matrix contact.
Cardeal, Laura Beatriz da Silva. "Caracterização de metaloproteinases de matriz e reck em queratinócitos primários que expressam oncoproteínas do papilomavírus humano (HPV)." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-28012011-113405/.
Повний текст джерелаCervical cancer is etiologically associated with to high-risk human papillomavirus (HPV) infection. It has been observed that matrix metalloproteinases (MMPs) -2, -9, and MT1-MMP are required for basement membrane degradation during cervical carcinoma progression. Moreover, a counterbalancing among MMPs and their regulators, such as TIMPs and RECK, is necessary to modulate invasion. In order to study the effect of HPV oncogenes on MMPs expression, primary human keratinocytes (PHKs) were infected with recombinant retroviruses expressing wild-type HPV16 E6 and/or E7 oncogenes and were used to seed monolayers and organotypic cultures. Quantitative real-time PCR (Q-PCR), western blot, zimography, immunocitochemistry, ELISA assay and immunohistochemistry were used to determine the expression level and activity of MMP-2, MMP-9, MT1-MMP and their inhibitors RECK and TIMP-2. We observed that cultures expressing E6E7 presented lower RECK and TIMP-2 protein levels than control keratinocytes. In addition, rafts cultures presented the same lower RECK levels additionally presenting higher MMP-9 activity than control. Furthermore, we observed that expression of E6 and/or E7 proteins do not affect MMP-2 and MT1-MMP protein levels and/or activity. We also observed that TNF treatment enhance the MMP-9 gene and protein expression and activity in all studied cell lines. Taken together, our results demonstrate that HPV16E6E7 expression is related with the unbalance between MMPs and their inhibitors, suggesting that in the initial steps of HPV-related cervical disease, not only MMPs but also RECK and TIMP-2 are critical for tumor progression.
Deng, Jie. "Rear Axle Gear Whine Noise Abatement via Active Vibration Control of the Rear Subframe." University of Dayton / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1447772359.
Повний текст джерелаSingh, Veena. "Evolutionary rearrangements in chloroplast genomes." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321027.
Повний текст джерелаDutreix, Marie. "Caractérisation des activités de la protéine RecA impliquées dans la réparation de l'ADN et la mutagénèse." Paris 11, 1988. http://www.theses.fr/1988PA112296.
Повний текст джерелаHobbs, Michael David. "On the regulation of RecA nucleoprotein filament formation by the RecF, RecO, RecR, RecX and SSB proteins : a biochemical analysis /." 2006. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаChien, Ya-Li, and 簡雅莉. "Helix-unwinding and single-strand DNA binding activities of escherichia coli RecF, RecO and RecR." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/63655641800884202892.
Повний текст джерела國立陽明大學
遺傳學研究所
84
recF,recO和recR基因是原先被發現參與RecF途徑之基因,目前有許多證據顯示此三個基因產物作用於DNA重組的同一步驟。現今有關RecF,RecO和RecR蛋白的在DNA重組途徑中所扮演的角色仍不清楚。 在本論文中,我們針對已純化的RecF,RecO和RecR蛋白的生化功能作進一步之探討,以期瞭解這些蛋白在DNA重組和修復上扮演的角色。主要的工作包括:(1)探討RecF,RecO和RecR與DNA結合之能力;(2)探討RecF,RecO和RecR的helix-unwinding活性。 首先,在RecF,RecO和RecR與DNA結合能力方面,我們證實單獨的RecF或RecO可以與ssDNA結合,而RecR不能。但在有RecO存在下,則RecR可與ssDNA結合。當RecF,RecO和RecR同時與ssDNA作用時,發現這三個蛋白皆可與ssDNA結合。 而在DNA unwinding活性方面,我們發現RecO有明確的活性,RecO的helix-unwinding活性需要有ATP及鎂離子,ATP之需求可以dATP取代,但無法以ATPγS或其它NTP替代,鎂離子之需求可以錳離子取代,但無法以鈣離子或鋅離子取代,RecOunwind helix的能力與RecO蛋白質之濃度成正比,當RecO之量可飽和結合至ssDNA時,其unwilnd DNA之活性最高,最後此活性會受到高濃度之鎂離子或鹽的抑制。根據我們發現RecO具有helix-unwinding活性,認為RecFOR在DNA重組作用中扮演新的角色。在本文中有將有詳細討論。 The recF, recO and recR genes were originally identified as those affecting the RecF pathways of recombination in Escherichia coli. Several lines of genetic evidence suggest that the recF, recO and recR gene products function at the same step of recombination, possibly at an early presynaptic step. The exact role of RecFOR in DNA recombination are not known. In this work, the interactions of RecF, RecO and RecR with ssDNA and the helix-unwinding activities of RecF, RecO and RecR were examined. We observed that single RecF or RecO can bind to ssDNA while single RecR cannot. In the presence of RecO, but not RecF, the RecR was found to associate with ssDNA. When the RecF, RecO and RecR were reacted with ssDNA, all three proteins were found to associate with DNA. With regards to helix-unwinding activity, we observed that RecO possesses such an activity. The helix-unwinding activity of RecO requires the presence of MgCl2 and ATP in the reaction mixture. The requirement of ATP can be substituted by dATP, but cannot be substituted by ATPγS or other NTPs. The requirement of magnesium can be substituted by manganese, but not by calcium or zinc. The unwinding activity is proportional to the concentration of RecO protein and is sensitive to high concentration of MgCl2 or NaCl. Maximal unwinding was observed when the RecO protein to DNA nucleotides was greater than 0.05. Our finding that RecO possesses helix-unwinding activity suggests a new role of ReeFOR in DNA recombination. A model for the possible involvement of RecFOR in DNA recombination is presented.
Su, Yi-Cheng, and 蘇宜成. "Expression studies of escherichia coli recR gene." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/31943983054646914897.
Повний текст джерела國立陽明大學
遺傳學研究所
82
recR基因是大腸桿菌細胞中與DNA重組有關的基因之一,其基因產物則為參與RecF重組途徑之作用成員之一。recR基因之發現雖然早在1989年,其選殖與定序亦於稍後陸續完成,但是關於Recf蛋白生化功能之研究與探討卻至今仍無明顯之進展。 本篇論文所探討的主題是recR基因之表現,目的是希望能大量表現RecR蛋白以利其純化及將來生化功能之探討。另外,本文也分析RecR蛋白質大量表現的情況下對於細胞生理功能之影響。為了達成上述目的,在我們的研究中選用兩種具有不同啟動子的表現載體:分別是具有Ptac啟動子的pKK388-1以及具有T7ψ10啟動子的PET-3d。並利用分子選殖技術將recR基因序列之結構部份接於兩種表現載體之上。 經由IPTG誘導後以SDS-PAGE分析細胞中RecR蛋白之表現,我們發現具有T7ψ10啟動子之表現載體可大量表現RecR,而其有Ptac啟動子的載體則僅微量表現RecR。另在誘導RecR蛋白表現的情況下,我們發現細胞之DNA修復作用及細胞存活性之變化與基礎表現狀態下比較並無明顯之差異。此結果顯示RecR之大量表現對於細胞之正常生理功能並無明顯之影響。 為進一步了解RecR蛋白之生化功能,我們從經IPTG誘導之細胞萃取液純化RecR。在經由Pheny1 sepharose CL-4B column以及PBE94 column加以純化之後,我們已可得到純度約80%的RecR蛋白質,此純化之RecR蛋白在體外DNA重組作用扮演之角色將於日後探討。 The recR gene was identified in 1989 as one of the RecF path-way recombination genes in Escherichia coli. Although the recR gene has been cloned and its product identified, the biochemical functions of RecR protein and its role in homologous recombination remain unknown. In this Study, the Structural portion of the recR gene was Synthesized by polymerase chain reaction (PCR) and cloned onto expression vectors which contain a Nco I fusion cloning site. Follow- ing IPTG induction, the expression of RecR was analyzed by SDS- polyacrylamide gel electrophoresis (SDS-PAGE). It was found that the recombinant plasmids carrying a T7 promoter (pVW62 and pVW63) overexpressed RecR in large quantity while the recombinant plasmid carrying a Ptac (pVW61) expressed RecR poorly. The effects of RecR overexpression on cell viability and U.V. Sensitivity were alSo examined. It was observed that the overexpression of RecR did not lead to cell death, nor did it affect the U.V. Sensitivity of uvrA recBC sbcBC recR cells. Finally, aS a prelude to understand the biochemical functions of RecR protein, we have purified RecR protein from cells which overexpressed RecR. After chromatography with Phenyl SepharoSe CL -4B and PBE 94, RecR with about 80% purity was obtained. Further works on the biochemical functions of RecR will be pursued.
Webb, Brian Lynn. "Characterization of the Escherichia coli RecF and RecR proteins and their role in recombinational DNA repair." 1997. http://catalog.hathitrust.org/api/volumes/oclc/39475544.html.
Повний текст джерелаChaudhary, Santosh Kumar. "Structural and functional studies on DNA synthesis and repair proteins." Thesis, 2018. https://etd.iisc.ac.in/handle/2005/5320.
Повний текст джерелаDrees, Julia C. "The novel RecA regulator proteins RecC DinI, RdgC and PsiB." 2006. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаRobu, Mara-Eliza. "Roles of RecA and RecG proteins in replication fork regression." 2003. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаPrasad, Deepika. "Regulation of RecA nucleoprotein filament stability by RecX and the effects of RecA-membrane interaction on the activities of RecA in mycobacteria." Thesis, 2018. https://etd.iisc.ac.in/handle/2005/5316.
Повний текст джерела"RECL 4 (=RECL 96) - 15-Aug-92." 1992. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/1130.
Повний текст джерела"RECL 191 -." 2011. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/543.
Повний текст джерела"RECL 263 -." 2011. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/849.
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Повний текст джерелаRajan, Prabhu J. "Structural Studies On Mycobacterial RecA And RuvA." Thesis, 2009. https://etd.iisc.ac.in/handle/2005/924.
Повний текст джерелаRajan, Prabhu J. "Structural Studies On Mycobacterial RecA And RuvA." Thesis, 2009. http://hdl.handle.net/2005/924.
Повний текст джерелаhong, wang jhen, and 王鎮宏. "The Role of Pet Dog in Leisure Life—The Rear Motivation、Rear Behavior and Rear Barrier of Pet Owner." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/59616203515593541161.
Повний текст джерела亞洲大學
休閒與遊憩管理學系碩士班
95
Dog’s utility is stressing on working functionally in elder human society. But with society changing ,people living style changing, and the quality of spirit lives raising, dog became merchandise ,the most loyal friend、companion、comforter, and a subject of playing. Raising of pet’s life value and characteristic, dog is not just a tool when people have time to perform hobby, its vitality and good well performance boost its value and importance, making people regard pets as accompany and personification, even making owner become pet’s parents. At the same time, this kind of relationship between pets and owner is closer, it is worthy to discuss and research. However, this research is based on quantification and deeply interview is auxiliary. Taichung city is the major base, there are 407 samples, deeply discussing pet’s role and importance in leisure time in order to understand the owner’s rearing motivation、rearing behavior, and rearing barrier that counteracts owner’s leisure time and influence. Based on theory of planned behavior, we discuss the causes that make owners to create rearing behavior. In addition, we also depend on different rearing motivation types and then distinguish those into many districts. In the end, this research we depend on SPSS 10.0 analysis result to make conclusion and proposition; it is hoped that the research data can be provided and consulted to related departments and industries when they are making policies or marketing programs.
候武勳. "Cloning of recF gene and construction of recF mutant of streptomyces lividans." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/06664026112073713600.
Повний текст джерелаChou, Chen-Ju, and 周辰儒. "Research on the rear-end collision avoidance and warning system by rear-end camera." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/10187206141637199024.
Повний текст джерела國立臺灣大學
土木工程學研究所
96
The objective of this study is to construct the rear-end collision avoidance and warning system of advance safety vehicle (ASV) by rear-end parking camera. Based on image processing technology to get and analyze the driving environmental data, we developed the rear-end collision warning logic and system. Since the automobile electronics industry developed faster, the parking assist system, like the ultrasonic sensors and rear-end camera, almost become the basic equipment in vehicles. However, the parking assist equipments are only turned on when in parking mode. Therefore, to provide the following vehicle information for subjective one, this study tends to apply the rear-end camera in vehicle when moving forward. It not only reduces the equipment cost of the vehicle but also increases the safety for drivers. The main idea of warning system is to prevent accidents which caused by inattentive of drivers. This study used the image processing to detect the longitudinal data of the following vehicle, including the relative distance, velocity, and acceleration. We developed the dynamic thresholds by the relative data and the drivers’ perceptive reaction time to issue the warning signal for drivers. The α-β-γ filter was applied in this section to get the smoother relative data. This study built up a rear-end collision avoidance and warning system by rear-end camera. We constructed the warning program by Borland C++ Builder. The warning hardware of the system was used the industry personal computer and on-board video equipment. The experiment result was successful for the off-line video test. In other words, this study proves that the developed rear-end warning system could appropriately issue the warning signal for drivers.
Chou, Chen-Ju. "Research on the rear-end collision avoidance and warning system by rear-end camera." 2008. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-3006200811031800.
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Повний текст джерела"RECL 185 - 4-Sep-94." 1994. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/519.
Повний текст джерела"RECL 186 - 17-Jul-85." 1985. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/523.
Повний текст джерела"RECL 188A - 30-Jul-86." 1986. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/530.
Повний текст джерела"RECL 189 - 10-Jul-86." 1986. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/534.
Повний текст джерела"RECL 191 - 9-Aug-85." 1985. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/540.
Повний текст джерела