Дисертації з теми "RecF"
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Raharjo, Joko Purnomo. "The performance analysis of real estate construction firms (RECF) in Indonesia." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/228029/1/Joko%20Purnomo_Raharjo_Thesis.pdf.
Повний текст джерелаTaylor, L. "The recB and recC gene products of Escherichia coli." Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355080.
Повний текст джерелаWilson, R. E. "The recB-recC region of the Escherichia coli chromosome." Thesis, University of Newcastle Upon Tyne, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375598.
Повний текст джерелаVickridge, Elise. "Management of E. coli sister chromatid cohesion in response to genotoxic stress." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS172/document.
Повний текст джерелаMaintaining genome integrity through replication is an essential process for the cell cycle. However, many factors can compromise this replication and thus the genome integrity. Mitomycin C is a genotoxic agent that creates a covalent link between the two DNA strands. When the replication fork encounters the DNA crosslink, it breaks and creates a DNA double strand break (DSB). Escherichia coli (E.coli) is a widely used model for studying complex DNA mechanisms. When facing a DNA DSB, E. coli activates the SOS response pathway. The SOS response comprises over 50 genes that are under the control of a LexA-repressed promoter. Upon a DSB induction, RecA, a central protein of the SOS response will trigger the degradation of LexA and all the SOS genes will be expressed.We have developed a novel molecular biology tool that reveals contacts between sister chromatids that are cohesive. It has been shown in the lab (Lesterlin et al. 2012) that during a regular cell cycle, the two newly replicated sister chromatids stay in close contact for 10 to 20 min before segregating to separate cell halves thanks to the action of Topoisomerase IV. This step is called sister chromatid cohesion. We have used this molecular biology tool to study sister chromatid cohesion upon a genotoxic stress induced by mitomycin C (MMC). We have shown that sister chromatid cohesion is maintained and prolonged when the cell is facing a DSB. Moreover, this sister chromatid cohesion is dependent on RecN, an SOS induced structural maintenance of chromosome-like (SMC-like) protein. In the absence of RecN, the proximity between both sister chromatids is lost and this has a deleterious effect on cell viability. By tagging the chromosome with fluorescent proteins, we have revealed that RecN can also mediated a progressive regression of two previously segregated sister chromatids and this is coordinated with a whole nucleoid compaction. Further studies showed that this genome compaction is orderly and is not the result of a random compaction in response to DNA damage.Interestingly, inhibiting TopoIV in a recN mutant fully restores viability and sister chromatid cohesion suggesting that RecN’s action is mainly structural. Preserving cohesion through precatenanes is sufficient to favor repair and cell viability even in the absence of RecN.An RNA-seq experiment in a WT strain and a recN mutant revealed that the whole SOS response is downregulated in a recN mutant. This suggests that RecN may have an effect on the induction of the SOS response and thus RecA filament formation. This is in good agreement with the change in RecA-mcherry foci formation we observed. In the WT strain, the RecA-mcherry foci are defined as described in previous work. However, in the recN, the RecA-mcherry foci seemed to form bundle like structures. These RecA bundles were previsously described by Lesterlin et al. in the particular case of a DSB occurring on a chromatid that has already been segregated from its homolog. This could mean that in the absence of recN, the sister chromatids segregate and RecA forms bundle like structures in order to perform a search for the intact homologous sister chromatid.Altogether, these results reveal that RecN is an essential protein for sister chromatid cohesion upon a genotoxic stress. RecN favors sister chromatid cohesion by preventing their segregation. Through a whole nucleoid rearrangement, RecN mediates sister chromatid regression, favoring DNA repair and cell viability
Dulermo, Rémi. "Etude des mécanismes de l'extrême tolérance aux radiations de la bactérie Deinococcus deserti par une approche de génomique fonctionnelle." Aix-Marseille 2, 2009. http://theses.univ-amu.fr.lama.univ-amu.fr/2009AIX22100.pdf.
Повний текст джерелаThe genome of Deinococcus deserti, a highly radiation-tolerant bacterium, was analyzed and compared to those of D. Radiodurans and D. Geothermalis. About 230 proteins are specifically conserved in these 3 species, including IrrE, a regulator protein essential for radiotolerance. D. Deserti has several supplementary DNA repair genes, like imuY and dnaE2 (translesion DNA polymerases). Moreover, D. Deserti has 3 recA that code for 2 different RecA proteins (RecAC et RecAP). To study these genes, genetic tools were developed for D. Deserti. Different results suggest that IrrE, required for the induction of several genes after irradiation, has peptidase activity. The 2 RecA proteins are functional for DNA repair. D. Deserti is mutable by UV, which requires ImuY, DnaE2 and RecAC, but not RecAP
Ames, Cory. "Reef Fish Assemblage Biogeography Along the Florida Reef Tract." NSUWorks, 2017. http://nsuworks.nova.edu/occ_stuetd/459.
Повний текст джерелаRech, Alexander [Verfasser]. "Werkwohnungen / Alexander Rech." Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2016. http://d-nb.info/1102805289/34.
Повний текст джерелаGeange, Shane Wallace. "An evaluation of prior residency and habitat effects on the persistence of settling reef fishes : a thesis submitted to the Victoria University of Wellington in fulfilment of the requirements for the degree of Doctor of Philosophy in Marine Biology /." ResearchArchive@Victoria e-Thesis, 2010. http://hdl.handle.net/10063/1169.
Повний текст джерелаScales, Helen Joanna. "Exploitation of coral reef fishes for the Live Reef Fish Trade." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.615075.
Повний текст джерелаNothdurft, Luke David. "Microstructure and early diagenesis of recent reef building scleractinian corals, Heron reef, Great Barrier Reef : implications for paleoclimate analysis." Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/16690/1/Luke_D._Nothdurft_Thesis.pdf.
Повний текст джерелаNothdurft, Luke David. "Microstructure and early diagenesis of recent reef building scleractinian corals, Heron reef, Great Barrier Reef : implications for paleoclimate analysis." Queensland University of Technology, 2008. http://eprints.qut.edu.au/16690/.
Повний текст джерелаStephenson, Christy Michelle. "Foraminiferal Assemblages on Sediment and Reef Rubble at Conch Reef, Florida USA." Scholar Commons, 2011. http://scholarcommons.usf.edu/etd/3367.
Повний текст джерелаKleinwächter, Lutz. "Reif für die Weltpolitik?" Universität Potsdam, 2010. http://opus.kobv.de/ubp/volltexte/2010/4162/.
Повний текст джерелаFuchs, Eran 1963. "Fluorescence in reef corals." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/8966.
Повний текст джерелаIncludes bibliographical references (p. 248-251).
Fluorescence can be a powerful tool for probing biological systems. Prior measurements from Caribbean corals identified five fluorescing pigments in reef corals. In this thesis I study coral fluorescence spectra. I wanted to learn if fluorescence could be useful for large scale mapping and monitoring of the reef as a part of an effort to stop the recently reported global decline in coral reefs condition. 3D excitation I emission spectra, average wavelength locations and shape variability studies of each of the pigments is presented. I also present an in situ corrununity study of the species Montastraea cavernosa and investigate the variability of fluorescence emission among colonies of one species at one location. Coral's fluorescence emission spectrum can result from the excitation of one or more fluorescing pigments. A mathematical algorithm was developed to separate coral fluorescence spectra into individual components. The un-mixing algorithm was combined with a prediction model whose purpose was to predict the response that will be produced by any excitation light source given knowledge of the response produced by a different light source. Energy coupling between two of the pigments was discovered. An empirical coupling efficiency factor was defined and calculated to account for this energy transfer. The energy coupling between these pigments may have important consequences in future investigation of coral's evolution. A new experimental method to separate the reflectance and fluorescence spectral components of fluorescing corals was developed for in vivo and in situ data. Two experimental methods are proposed to measure and calculate a newly defined quantity, "practical fluorescence efficiency". This efficiency factor is essential for correct prediction of coral spectra under different illumination conditions. This part of my work will benefit optical models that calculate light interaction with the bottom of the ocean in shallow waters. Lastly I present a prototype Fluorescence Imaging Laser Line Scanner system and discuss its potential use as a remote sensing system for reef mapping and monitoring. Recommendations are made to better tune the system to the fluorescence characteristics of reef corals.
by Eran Fuchs.
Ph.D.
Wormald, Clare Louise. "Effects of density and habitat structure on growth and survival of harvested coral reef fishes /." View online ; access limited to URI, 2007. http://0-digitalcommons.uri.edu.helin.uri.edu/dissertations/AAI3277014.
Повний текст джерелаCeh, Janja. "Coral-associated microbial communities in reef-building corals of Ningaloo Reef Western Australia." Thesis, Ceh, Janja (2011) Coral-associated microbial communities in reef-building corals of Ningaloo Reef Western Australia. PhD thesis, Murdoch University, 2011. https://researchrepository.murdoch.edu.au/id/eprint/8480/.
Повний текст джерелаAaby, Alyssa Anne. "Testing the ArcGIS Marine Data Model : using spatial information to examine habitat utilization patterns of reef fish along the west coast of Hawaii /." Connect to this title online, 2004. http://hdl.handle.net/1957/4061.
Повний текст джерелаFisco, Dana. "Reef Fish Spatial Distribution and Benthic Habitat Associations on the Southeast Florida Reef Tract." NSUWorks, 2016. http://nsuworks.nova.edu/occ_stuetd/408.
Повний текст джерелаHobbs, Michael David. "On the regulation of RecA nucleoprotein filament formation by the RecF, RecO, RecR, RecX and SSB proteins : a biochemical analysis /." 2006. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаChien, Ya-Li, and 簡雅莉. "Helix-unwinding and single-strand DNA binding activities of escherichia coli RecF, RecO and RecR." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/63655641800884202892.
Повний текст джерела國立陽明大學
遺傳學研究所
84
recF,recO和recR基因是原先被發現參與RecF途徑之基因,目前有許多證據顯示此三個基因產物作用於DNA重組的同一步驟。現今有關RecF,RecO和RecR蛋白的在DNA重組途徑中所扮演的角色仍不清楚。 在本論文中,我們針對已純化的RecF,RecO和RecR蛋白的生化功能作進一步之探討,以期瞭解這些蛋白在DNA重組和修復上扮演的角色。主要的工作包括:(1)探討RecF,RecO和RecR與DNA結合之能力;(2)探討RecF,RecO和RecR的helix-unwinding活性。 首先,在RecF,RecO和RecR與DNA結合能力方面,我們證實單獨的RecF或RecO可以與ssDNA結合,而RecR不能。但在有RecO存在下,則RecR可與ssDNA結合。當RecF,RecO和RecR同時與ssDNA作用時,發現這三個蛋白皆可與ssDNA結合。 而在DNA unwinding活性方面,我們發現RecO有明確的活性,RecO的helix-unwinding活性需要有ATP及鎂離子,ATP之需求可以dATP取代,但無法以ATPγS或其它NTP替代,鎂離子之需求可以錳離子取代,但無法以鈣離子或鋅離子取代,RecOunwind helix的能力與RecO蛋白質之濃度成正比,當RecO之量可飽和結合至ssDNA時,其unwilnd DNA之活性最高,最後此活性會受到高濃度之鎂離子或鹽的抑制。根據我們發現RecO具有helix-unwinding活性,認為RecFOR在DNA重組作用中扮演新的角色。在本文中有將有詳細討論。 The recF, recO and recR genes were originally identified as those affecting the RecF pathways of recombination in Escherichia coli. Several lines of genetic evidence suggest that the recF, recO and recR gene products function at the same step of recombination, possibly at an early presynaptic step. The exact role of RecFOR in DNA recombination are not known. In this work, the interactions of RecF, RecO and RecR with ssDNA and the helix-unwinding activities of RecF, RecO and RecR were examined. We observed that single RecF or RecO can bind to ssDNA while single RecR cannot. In the presence of RecO, but not RecF, the RecR was found to associate with ssDNA. When the RecF, RecO and RecR were reacted with ssDNA, all three proteins were found to associate with DNA. With regards to helix-unwinding activity, we observed that RecO possesses such an activity. The helix-unwinding activity of RecO requires the presence of MgCl2 and ATP in the reaction mixture. The requirement of ATP can be substituted by dATP, but cannot be substituted by ATPγS or other NTPs. The requirement of magnesium can be substituted by manganese, but not by calcium or zinc. The unwinding activity is proportional to the concentration of RecO protein and is sensitive to high concentration of MgCl2 or NaCl. Maximal unwinding was observed when the RecO protein to DNA nucleotides was greater than 0.05. Our finding that RecO possesses helix-unwinding activity suggests a new role of ReeFOR in DNA recombination. A model for the possible involvement of RecFOR in DNA recombination is presented.
候武勳. "Cloning of recF gene and construction of recF mutant of streptomyces lividans." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/06664026112073713600.
Повний текст джерелаWebb, Brian Lynn. "Characterization of the Escherichia coli RecF and RecR proteins and their role in recombinational DNA repair." 1997. http://catalog.hathitrust.org/api/volumes/oclc/39475544.html.
Повний текст джерела曾玉琴. "Grouping of RecF pathway recombination genes in escherichia coli." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/80502672342493549135.
Повний текст джерелаLee, Chuan-Chan, and 李權展. "Construction and characterization of recF mutants of streptomyces lividans 66 ZX7." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/86231224343043581087.
Повний текст джерелаWen, Chen-Yu, and 溫振宇. "Regulation and Biological Functions of R-loop: Roles of RecF Pathway and DNA Topoisomerases." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/23217513442238525029.
Повний текст джерела國立臺灣大學
微生物學研究所
97
The R-loop structure, an RNA-DNA hybrid with a single-stranded DNA region, has been observed during transcription elongation. However, the regulation and biological functions of R-loop still remain largely unknown. In this study, we took advantage of the bacterial genetic model system to solve these problems. We have found that several cellular factors, such as RecF, RNase H and DNA topoisomerases, are involved in the regulation of R-loop formation and thereby modulating its associated cellular functions. Forced expression of human activation-induced deaminase (hAID) in bacteria was utilized to stimulate mutagenesis and the stimulated folds were then used to represent the different levels of R-loop in various strains and conditions. Specifically, the involvement of RecF pathway is supported by the following observations: (i) The fold of AID-stimulated mutagenesis (ASM) in RecF-activated strain JC7623 (recBC sbcBC) was much higher than those measured in other related mutants; furthermore, additional expression of RNase H or TopA suppressed the ASM fold. (ii) Plasmid-mediated lethality, run-away plasmid replication and cellular filamentation phenotype were also exclusively observed in JC7623 cells. (iii) Most importantly, all these phenotypes could be suppressed by ectopic expression of functional RNase H and TopA. (iv) Over-expression of TopA and gyrase reduced and enhanced the filamentation phenotype in RecF-activated bacteria, respectively. In sum, our results suggested that formation of excess R-loops contributes to all the phenotypes observed in RecF-activated JC7623 cells. Furthermore, we have also revealed many novel functions of R-loop in recombination, DNA replication, cellular filamentation and lethality.
Drees, Julia C. "The novel RecA regulator proteins RecC DinI, RdgC and PsiB." 2006. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаRobu, Mara-Eliza. "Roles of RecA and RecG proteins in replication fork regression." 2003. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерела"RECL 4 (=RECL 96) - 15-Aug-92." 1992. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/1130.
Повний текст джерела"RECL 191 -." 2011. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/543.
Повний текст джерела"RECL 263 -." 2011. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/849.
Повний текст джерела"RECL 50A -." 2011. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/1160.
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Повний текст джерелаHempson, Tessa N. "Coral reef mesopredator trophodynamics in response to reef condition." Thesis, 2017. https://researchonline.jcu.edu.au/53079/19/53079-hempson-2017-thesis.pdf.
Повний текст джерелаBierwagen, Stacy L. "Functional roles of reef sharks on the Great Barrier Reef." Thesis, 2019. https://researchonline.jcu.edu.au/64966/1/JCU_64966_bierwagen_stacy_2019_thesis.pdf.
Повний текст джерелаDrew, Christina Ashton. "Spatial ecology of reef fish in backreef and coral reef habitats." 2006. http://www.lib.ncsu.edu/theses/available/etd-04172006-133049/unrestricted/etd.pdf.
Повний текст джерелаHou, Shang-Ju, and 侯尚儒. "Coral Reef Patch Mapping." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/78553194759526420403.
Повний текст джерела國立交通大學
土木工程系所
102
Coral reef is formed by the corals’ growth and accumulation. According to the geography, it can be divided into two parts: the flat and the slope. By its construction, coral reef can be divided into three types: the fringing reef, the barrier reef and the atoll reef. The atoll reef areas on Dongsha Island are what being researched in this study, and this research aims at detecting and drawing the location of reefs around the Dongsha atoll area. The data used in this research includes digital elevation model (DEM) and satellite images. Three 1/5000 drawings had been chosen as the researched area depending on the characteristics of terrain, and their locations are: the middle of the lagoon area, the junction of the lagoon and the reef plat, and the reef plat. Trend surface analysis and object based segmentation are the two reef location-detecting ways that this research uses. The first one can only detect the location of reefs in DEM, while the second can detect both in DEM and the satellite images. The detected results will be compared with the real situation basing on the human observation of DEM and the sketched map of the reefs distribution. According to the different ways and combinations, it proves that object based segmentation in combination of the image of DEM, serves best. Among the five different analyses, the best one is DEM weight of 4 and image weight of 1. The average accuracy of the three areas is above 0.97, and the Kappa coefficient is higher than 0.84.
Hubble, Marc. "The ecological significance of body size in tropical wrasses(Pisces : Labridae)." Thesis, 2003. https://researchonline.jcu.edu.au/81/1/01front.pdf.
Повний текст джерелаHubble, Marc. "The ecological significance of body size in tropical wrasses (Pisces: Labridae) /." 2003. http://eprints.jcu.edu.au/81.
Повний текст джерелаBay, Line K. "The population genetic structure of coral reef fishes on the Great Barrier Reef /." 2005. http://eprints.jcu.edu.au/14.
Повний текст джерелаBay, Line Kolind. "The population genetic structure of coral reef fishes on the Great Barrier Reef." Thesis, 2005. https://researchonline.jcu.edu.au/14/1/01front.pdf.
Повний текст джерелаHill, Jocelyn K. "Reef Check benthic survey error: a case study on the Great Barrier Reef." Thesis, 2002. https://researchonline.jcu.edu.au/47504/1/47504-hill-2002-thesis.pdf.
Повний текст джерелаHall, Vicki R. "Injury and regeneration of common reef-crest corals at Lizard Island, Great Barrier Reef /." 1998. http://eprints.jcu.edu.au/8.
Повний текст джерелаHall, Vicki R. "Injury and regeneration of reef-crest corals at Lizard Island, Great Barrier Reef, Australia." Thesis, 1998. https://researchonline.jcu.edu.au/8/1/01front.pdf.
Повний текст джерела"RECL 1 - 25-Aug-87." 1987. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/490.
Повний текст джерела"RECL 180 - 8-Aug-85." 1985. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/500.
Повний текст джерела"RECL 180 - 7-Aug-88." 1988. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/501.
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Повний текст джерела"RECL 183 - 11-Jul-86." 1986. http://biblio-dev.laurentian.ca:8180/jspui/handle/10219/511.
Повний текст джерела