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Добірка наукової літератури з теми "Real-time cell analysis"

Автор: Grafiati

Опубліковано: 9 листопада 2024

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Статті в журналах з теми "Real-time cell analysis"

Vogt, Nina. "Real-time behavioral analysis." Nature Methods 18, no. 2 (February 2021): 123. http://dx.doi.org/10.1038/s41592-021-01072-z.

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Pranada, Albert L., Silke Metz, Andreas Herrmann, Peter C. Heinrich, and Gerhard Müller-Newen. "Real Time Analysis of STAT3 Nucleocytoplasmic Shuttling." Journal of Biological Chemistry 279, no. 15 (December 29, 2003): 15114–23. http://dx.doi.org/10.1074/jbc.m312530200.

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Jaiswal, Devina, Armin Tahmasbi Rad, Mu-Ping Nieh, Kevin P. Claffey, and Kazunori Hoshino. "Micromagnetic Cancer Cell Immobilization and Release for Real-Time Single Cell Analysis." Journal of Magnetism and Magnetic Materials 427 (April 2017): 7–13. http://dx.doi.org/10.1016/j.jmmm.2016.11.002.

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Fregin, Bob, Fabian Czerwinski, Doreen Biedenweg, Salvatore Girardo, Stefan Groß, Konstanze Aurich, and Oliver Otto. "Dynamic Real-Time Deformability Cytometry - Time-Resolved Mechanical Single Cell Analysis at 100 Cells/s." Biophysical Journal 118, no. 3 (February 2020): 605a. http://dx.doi.org/10.1016/j.bpj.2019.11.3267.

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Cerignoli, Fabio, Yama A. Abassi, Brandon J. Lamarche, Garret Guenther, David Santa Ana, Diana Guimet, Wen Zhang, Jing Zhang, and Biao Xi. "In vitro immunotherapy potency assays using real-time cell analysis." PLOS ONE 13, no. 3 (March 2, 2018): e0193498. http://dx.doi.org/10.1371/journal.pone.0193498.

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Rappoport, J. Z. "Real-time analysis of clathrin-mediated endocytosis during cell migration." Journal of Cell Science 116, no. 5 (January 15, 2003): 847–55. http://dx.doi.org/10.1242/jcs.00289.

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Ersöz, M., S. Malkoç, EB Küçük, BS Bozkurt, and SS Hakki. "Biocompatibility evaluation of orthodontic composite by real-time cell analysis." Human & Experimental Toxicology 35, no. 8 (July 19, 2016): 833–38. http://dx.doi.org/10.1177/0960327115607944.

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Introduction: The aim of this study was to evaluate the cytotoxic effects of three different light-cured orthodontic composites. Material and methods: Light Bond (Reliance orthodontic products), Grengloo (Ormco corporation), and Kurasper F (Kuraray Europe GmbH) were selected for the experiment. Specimens were prepared according to the manufacturers’ instructions, measuring 5 mm in diameter and 2 mm in thickness. Fibroblast cells were obtained from healthy gingival connective tissues. The composite cylinders were incubated in Dulbecco’s modified Eagle’s culture medium for 72 h according to ISO 10993-5 standards. The xCELLigence method was used to evaluate fibroblast cell vitality. After seeding 200 mL of the cell suspensions into the wells (20,000 cells/well) of the E-plate 96, gingival fibroblasts were treated with bioactive components released by the orthodontic composite materials and monitored every 15 min for 121 h. Results: There were no significant differences between the human gingival fibroblast (HGF) cell indexes of the control and all testing groups ( p > 0.05) at 24 and 48 h. Light Bond demonstrated statistically significant decrease in HGF index ( p < 0.05) at 72 h, but there was no significant difference among the Kurasper F, Grengloo, and untreated control groups ( p > 0.05). Light Bond ( p < 0.001) and Grengloo ( p < 0.05) groups had lower HGF cell index values when compared to untreated control group, but Kurasper F demonstrated no significant differences between the control groups at 96 h ( p > 0.05). Conclusion: Orthodontic composite materials include biologically active components and may change oral tissue. So, biocompatible orthodontic bonding composites should be used.

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Simons, Peter C., Sean M. Biggs, Anna Waller, Terry Foutz, Daniel F. Cimino, Qing Guo, Richard R. Neubig, Wei-Jen Tang, Eric R. Prossnitz, and Larry A. Sklar. "Real-time Analysis of Ternary Complex on Particles." Journal of Biological Chemistry 279, no. 14 (January 15, 2004): 13514–21. http://dx.doi.org/10.1074/jbc.m310306200.

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Mazhar, Muhammad Waqar. "Molecular Analysis of Covid-19 Patient Real Time PCR and their Medicational Clinical Trials." Virology & Immunology Journal 5, no. 3 (August 2, 2021): 1–4. http://dx.doi.org/10.23880/vij-16000284.

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The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to corona viradae family and nideo virales order, envelop virus, size range 65-125nm and positive single standard RNA between 26.4 to 31.7 kb and contain7096 amino acid. There are four subtypes that have been detected these are alpha, beta, gamma and delta. The 267 covid -19 blood and nasopharyngeal samples were collected from Multan region. RNA extraction from nasopharyngeal samples and run the PCR. The blood samples use for clinical tests, Lactate dehydrogenase, serum ferritin level, D-Dimer, TG, cholesterol, thyphoidot, HDL, lymphocyte count and CRP. The 127 (47.21%) out of 267 patients were covid-19 PCR positive and showed the amplification ORF1ab, E and N gene while140 individuals were covid-19 PCR negative and not showed the amplification of ORF1ab, E and N gene. The patients with negative Covid-19 PCR, the other analysis tests such as lactate dehydrogenase, HDL, ferritin, ESR,CBP, D. Dimer, Tg, cholesterol, CRP and CT scan. The patients affected covid-19 has higher values of D-Dimer, ESR, Neutrophils, LDH, CRP and ferritin level than normal ranges. But the values of the HDL, cholesterol and lymphocytes were decreased form the normal ranges. Drugs are mentioned in table #3 that is treating for covid 19- patients. These drugs are successful for Covid -19 treatments.

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Aoyama, Tadayoshi, Amalka De Zoysa, Qingyi Gu, Takeshi Takaki, and Idaku Ishii. "Vision-Based Real-Time Microflow-Rate Control System for Cell Analysis." Journal of Robotics and Mechatronics 28, no. 6 (December 20, 2016): 854–61. http://dx.doi.org/10.20965/jrm.2016.p0854.

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[abstFig src='/00280006/09.jpg' width='300' text='Snapshots of particle sorting experiment using our system' ] On-chip cell analysis is an important issue for microtechnology research, and microfluidic devices are frequently used in on-chip cell analysis systems. One approach to controlling the fluid flow in microfluidic devices for cell analysis is to use a suitable pumps. However, it is difficult to control the actual flow-rate in a microfluidic device because of the difficulty in placing flow-rate sensors in the device. In this study, we developed a real-time flow-rate control system that uses syringe pumps and high-speed vision to measure the actual fluid flow in microfluidic devices. The developed flow-rate control system was verified through experiments on microparticle velocity control and microparticle sorting.

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Дисертації з теми "Real-time cell analysis"

Hong, Soonjin Barbee Kenneth A. "Quantitative analysis of cell-surface interactions and cell adhesion process in real-time /." Philadelphia, Pa. : Drexel University, 2008. http://hdl.handle.net/1860/2840.

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Schulz, Craig. "Microsequential injection systems for the real-time monitoring of glucose metabolism of live cells by enzymatic assay /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/8694.

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Maiuri, Paolo. "Single-cell and real-time analysis of transcription rates from integrated HIV-1 provirus." Doctoral thesis, Scuola Normale Superiore, 2009. http://hdl.handle.net/11384/85935.

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Viral RNA biogenesis is a crucial step in the replication of retroviruses that require both the production of a genomic RNA as well as of translation templates. Cellular and viral factors concur in the biogenesis of RNA at the specific sub-nuclear chromatin site where the reaction takes place. The possibility of tracking viral RNA in living cells gives the unique possibility of measuring the kinetic parameters of RNA biogenesis as well as defining the dynamic recruitment of host and viral factors to the site of replication. In order to study the activation of HIV-1 gene expression from the integrated viral promoter we exploited a method that allows the visualization of newly transcribed RNA in living cells through the specific recognition of an RNA consensus sequence for the bacteriophage MS2 coat protein tagged with an autofluorescent protein. We observed that transcription of HIV-1 occurred in discrete foci within the nucleus of cells carrying several tandem arrays of the HIV-1 construct. These foci, representing newly transcribed RNA, co-localized with the viral Tat transactivator as well as members of the positive transcription elongation factor (P-TEFb) and RNA polymerase II (RNAPII). This experimental setting was used to measure the dynamic of HIV-1 RNA transcription in living cells. By fluorescence recovery after photobleaching (FRAP) we were able to demonstrate that following photobleaching the process reaches a steady state with a negligible immobile fraction allowing precise kinetic measurements of RNA polymerase elongation rates. We found that elongation proceeded at approximately 2 kb/min, and that 3'-end formation and release took another minute to complete. In addition we also analyzed the dynamic of RNAPII and the TAR:Tat:pTEFb complex at the site of HIV-1 transcription in living cells. Our data suggest that, while the residence time of RNAPII exceeds the time required for elongation through the viral template, the complex dissociates from the polymerase following transcription initiation, and may undergo subsequent cycles of association/dissociation. This approach was extended to the analysis of single integrated HIV- 1 transcription units by transduction of a HIV-based lentiviral vector into a human cell line and subsequent selection for Tat-induction from a latent state. Nascent RNAs from single integrated transcription units were detectable in living cells by MS2 RNA-tagging. At steady state a constant number of RNAs was measured at the transcription site corresponding to a minimal density of polymerases with negligible fluctuations over time both in space and intensity of the signal. Recovery of fluorescence after photobleaching of the transcription site was complete within seconds, much faster than what was observed previously. However, the necessity of taking into account also the diffusion of the tagged MS2 protein required the development of novel analytical tools. To this end we developed a model that describes each polymerase sliding along the DNA like the peak of a positive progressive traveling wave (TranWave) to predict the number of MS2 RNA repeats at the transcription site in function of time. The outcome of this approach and its following improvements are being discussed. This work provides for the first time a kinetic framework to analyze HIV-1 RNA biogenesis and RNA/protein dynamics in living cells.

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Shoshi, Astrit [Verfasser]. "Magnetic lab-on-a-chip for cell analysis : magnetoresistive-based real-time monitoring of dynamic cell-environment interactions / Astrit Shoshi." Bielefeld : Universitaetsbibliothek Bielefeld, 2013. http://d-nb.info/1036974502/34.

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Li, Min. "Kinetic analysis of Human T-cell leukemia virus type 1 gene expression." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1228156327.

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Zhou, Daming. "Modeling and Multi-Dimensional Analysis of a Proton Exchange Membrane Fuel Cell." Thesis, Bourgogne Franche-Comté, 2017. http://www.theses.fr/2017UBFCA011/document.

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Un des freins à la commercialisation de masse de la pile à combustible et notamment de la technologie à membrane échangeuse de proton vient de sa faible durée de vie due à la difficulté de contrôler le système sous certaines conditions. Pour pallier à ce problème, l’élaboration d’un modèle mathématique précis de la pile à combustible à membrane échangeuse de protons permettant d’observer les variables internes et l'état de la pile à combustible au cours de son fonctionnement permettrait le développement de la stratégie de contrôle du système.Cette thèse propose d’élaborer un modèle dynamique multi-physique complet pour la pile à combustible à membrane échangeuse de protons. Le modèle proposé couvre les domaines multi-physiques pour les caractéristiques électriques, fluidiques et thermiques. Dans ces deux derniers domaines, les phénomènes transitoires sont notamment pris en compte dans le modèle proposé, tels que les comportements dynamiques de la teneur en eau de la membrane de la pile et la température. Par conséquent, ce modèle peut être utilisé pour analyser les effets de couplage des variables dynamiques entre différents domaines physiques.Grace à ce modèle ainsi définit, un second modèle multi-physique bidimensionnel plus détaillé est également présenté. Le modèle proposé couvre les domaines électriques et fluidiques avec une approche de modélisation 2-D innovante. Les distributions spatiales de quantité physique dans le domaine électrique peuvent ainsi être obtenues. Par conséquent, ce modèle 2-D PEMFC peut être utilisé pour étudier les influences des paramètres de modélisation sur la prédiction de performance multidimensionnelle locale. Une étude expérimentale est effectuée pour valider le modèle 2-D proposé avec une pile commerciale PEMFC Ballard NEXA de 1,2 kW.Dans un second chapitre, une analyse des phénomènes dynamiques est réalisée en fonction du modèle dynamique multidisciplinaire développé en s’appuyant sur la méthode RGA (gain relatif) pour diverses variables d'entrée de contrôle, afin d'analyser quantitativement les effets de couplage dans différents domaines physiques. L’étude s’intéresse entre autre aux interactions de la teneur en eau et de la température de la membrane. L'analyse de couplage présentée dans cette thèse peut aider les ingénieurs à concevoir et à optimiser les stratégies de contrôle des piles à combustible, en particulier pour la gestion de l'eau et de la chaleur dans les systèmes de piles à combustible.Une deuxième analyse portant sur la sensibilité aux paramètres de l'étude est effectuée sur la base du modèle multidisciplinaire bidimensionnel développé. Ces résultats d'analyse de sensibilité globale fournissent des informations utiles pour la compréhension de la dégradation, le réglage des paramètres et la simplification du modèle des piles à combustible.Dans un troisième temps, le modèle proposé se décline dans un algorithme de résolution mathématique en temps réel basé sur un algorithme de matrice tri diagonal efficace (TDMA). Les résultats expérimentaux démontrent les possibilités pratiques du modèle 2-D proposé pour le contrôle en temps réel avancé des systèmes de pile à combustible avec un temps de calcul de la boucle de contrôle de l'ordre de la milliseconde. Le temps d'exécution du modèle peut être quadruplé par rapport aux algorithme séquentiels présent dans la littérature; garantissant ainsi des décisions et des actions de contrôle rapide
Before mass commercialization of proton exchange membrane fuel cell, the research on the design of appropriate control strategies and auxiliaries need to be done for achieving proton exchange membrane fuel cell (PEMFC) optimal working modes. An accurate mathematical PEMFC model can be used to observe the internal variables and state of fuel cell during its operation, and could further greatly help the system control strategy development.A comprehensive multi-physical dynamic model for PEMFC is developed in chapter I. The proposed model covers multi-physical domains for electric, fluidic and thermal features. Particularly, the transient phenomena in both fluidic and thermal domain are simultaneously considered in the proposed model, such as the dynamic behaviors of fuel cell membrane water content and temperature. Therefore, this model can be used to analyze the coupling effects of dynamic variables among different physical domains.Based on the developed multi-physical PEMFC model, a full two-dimensional multi-physical model is further presented. The proposed model covers electrical and fluidic domains with an innovative 2-D modeling approach. In order to accurately describe the characteristics of reactant gas convection in the channels and diffusion through the gas diffusion layer, the gas pressure drop in the serpentine pipeline is comprehensively analyzed by fully taking the geometric form of flow field into consideration, such as the reactant gas pressure drop due to the pipeline sharp and U-bends. Based on the developed 2-D fluidic domain modeling results, spatial physical quantity distributions in electrical domain can be further obtained. Therefore, this 2-D PEMFC model can be use to study the influences of modeling parameters on the local multi-dimensional performance prediction. The simulation and experimental test are then performed to validate the proposed 2-D model with a commercial Ballard NEXA 1.2 kW PEMFC stack.In chapter II, analyses of dynamic phenomena step responses are conducted based on the developed multi-physical dynamic PEMFC model using the relative gain array (RGA) method for various control input variables, in order to quantitatively analyze the coupling effects in different physical domains, such as the interactions of membrane water content and temperature. Based on the calculated values of relative gain array, the proposed model can be considered as a fuel cell MIMO system, which could be divided into two independent control sub-systems by minimizing parameter coupling effects between each other. Due to the closely coupled parameters in the proposed first control sub-system, a decoupling control method is recommended to achieve optimized control results. The coupling analysis presented in this thesis can help engineers to design and optimize the fuel cell control strategies, especially for the water and thermal management in fuel cell systems

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Czerwieniec, Gregg Allen. "Single cell analysis using bio-aerosol mass spectrometry : development and applications for the real-time detection of bio-warfare pathogens /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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AL, DOSSARY REEM. "Activation of human endogenous retrovirus K and cellular modifications in human melanoma cell lines: gene expression analysis." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2010. http://hdl.handle.net/2108/1389.

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Presso i laboratori dell’Università di Tor Vergata è stato sviluppato un sistema in vitro dotato di caratteristiche idonee allo studio dei meccanismi che sono alla base dello sviluppo e della progressione del melanoma. Tale sistema è costituito da una linea cellulare di melanoma umano, isolata da una lesione metastatica di una paziente e caratterizzata dalla capacità di crescere in adesione (linea TVM-A12), da cui sono state ottenute due distinte linee cellulari, dotate invece della caratteristica di crescita in sospensione, derivate l’una mediante tecnica della diluizione limite (Clonesp), e l’altra riducendo la concentrazione di siero dal 10% al 1% nel terreno di coltura (linea TVM-A12sp). Il passaggio dalla fase di crescita in adesione a quella in sospensione, osservato quando le cellule TVM-A12 erano poste in condizione di ridotta concentrazione di siero (1%) era accompagnato da riduzione di espressione dell’antigene melanocitico Melan-A/MART-1 e delle molecole HLA di classe I oltre che dall’aumentata capacità di formare colonie in terreno semisolido. Il cambiamento nella modalità di crescita delle cellule risultava inoltre associata all’aumento dell’espressione di HERV-K, sia a livello di mRNA che di proteine e poteva essere inibito dal blocco dell’espressione di HERV-K, ottenuto mediante RNA interference. Scopo del presente lavoro è stato quello di caratterizzare le linee cellulari TVM-A12, parentale e di derivazione, dotate rispettivamente di capacità di crescita in aderenza ed in sospensione, al fine di chiarire i meccanismi che sono alla base dell’acquisizione del fenotipo dotato di maggiore aggressività ed individuare il ruolo dell’attivazione di HERV-K nella progressione del melanoma. A tale scopo, è stato condotto uno studio dell’espressione genica, mediante microarray, seguito da analisi in Real-Time PCR, che ha mostrato come la transizione fenotipica delle cellule, dalla fase di crescita in adesione a quella di crescita in sospensione, risultava accompagnata dalla modulazione di numerosi geni, che è noto essere coinvolti nell’acquisizione di caratteristiche di malignità. Inoltre il profilo di espressione delle cellule a crescita in sospensione, siano esse originate per diluizione limite o per riduzione della concentrazione di siero nel mezzo di coltura, risultava essere del tutto simile. Lo studio in Real-Time, utilizzato per confermare quanto osservato mediante microarray, ha mostrato che quando le cellule di melanoma iniziavano a distaccarsi dal monostrato, in presenza di FBS all’1%, i geni BHLHB2 e MYC risultavano transitoriamente attivati. Nelle cellule in presenza di bassa concentrazione di siero e durante il passaggio verso la fase definitiva di crescita in sospensione, i geni PTEN, VEFGA, CSK, PITCH1, FOXG1A e TP53 risultavano progressivamente up-regolati. Nelle cellule di melanoma che avevano acquisito la capacità di crescere stabilmente in sospensione, si osservava infine aumento di espressione dei geni WNT3, MYCN, MYCL1, BTK, CCND2, WNT2, TIMP3, IRF3, GTF2I, CTNNB1, E2F1, ARHGAP5, ARHGEF5, GPR39 e ITGB4. Riduzione di espressione dei geni ANXA7, CTNNA1, NME1, RRM1, CDKN1A, XRCC6, HDAC, TRAM1, CD59 e TOB1 veniva riscontrata invece nelle cellule ancora adese, in presenza di 1 % di siero ed in quelle già in sospensione, rispetto alla linea di origine mantenuta in condizioni di coltura standard (10%FBS). In questo studio è stato per la prima volta descritto un sistema cellulare in cui è stata dimostrata la correlazione tra aumento di espressione di HERV-K e acquisizione di un fenotipo più aggressivo, nonché la modulazione dei geni che in tali processi risultano coinvolti. Tale modello può fornire pertanto un utile strumento per la comprensione dei meccanismi che regolano lo sviluppo e la progressione del melanoma, nonché per la valutazione di agenti farmacologici e modulatori di geni attivi nei confronti dell’espressione di HERV-K e nella progressione del melanoma.

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Boussemaere, Luc. "Investigating off-axis digital holographic microscopy with a source of partial spatial coherence as a real-time sensor for cell cultures." Doctoral thesis, Universite Libre de Bruxelles, 2015. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209086.

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Bio-pharmaceutical industry is a vast growing market and recent recommendations of the Food and Drug Administration have put a large emphasis on the characterization of biological processes and models. As a consequence, there is a high incentive on developing modern sensors in order to more accurately monitor and control processes. In that way, Digital Holographic Microscopy (DHM) presents unique features thanks to the refocusing and quantitative phase contrast imaging capabilities. In this thesis we investigate the usage of DHM to monitor yeast cultures that are often used in both the bio-pharmaceutical and bread industries and lay the basis of a methodological framework for the study of in-line cell cultures in the context of process control. We begin with a description of Digital Holography and the microscopy setup used in the thesis as well as a detailed explanation of the image processing required to extract the holographic data and its implementation on GPU with some speed execution figures given for three popular programming paradigms. We then describe the flow setup used and infer the limitations on the dynamic range of the technique due to both Poisson statistics and overlapping phenomena. Finally, we describe an algorithm that extracts the cells position, count and morphological information such as the size, aspect ratio, circularity and refraction index. Some experimental results are presented for yeasts before drawing a general overview of the technology and its dependencies. We further end with some conclusions concerning the technology and a brief comparison with existing competitors.
Doctorat en Sciences de l'ingénieur
info:eu-repo/semantics/nonPublished

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Pretorius, Ashley. "Functional analysis of the mouse RBBP6 gene using Interference RNA." Thesis, University of the Western Cape, 2007. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4435_1264363734.

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The aim of this thesis was to investigate the cellular role of the mouse RBBP6 gene using the interference RNA (RNAi) gene targeting technology and also to understand the relevance of two promoters for the RBBP6 gene.

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Книги з теми "Real-time cell analysis"

SAHAIDAK, Mykhailo, ed. STRATEGIC IMPERATIVES OF MODERN MANAGEMENT. Kyiv National Economic University named after Vadym Hetman, 2024. http://dx.doi.org/10.35668/978-966-926-500-5.

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This study examines issues of modern management and trends in its development. Its evolution from the end of the 19th century to the present is presented. The current state of management systems is analyzed, attention is paid to trends in the development of management science and practice, which have developed and are still being formed, as well as objective factors that affect the specified process. Globalization, as a phenomenon, is a complex and multifaceted process that affects various aspects of society, economy, and politics. In the context of business and management, globalization creates new opportunities for the development of international businesses, but at the same time poses challenges and threats associated with competition, cultural differences, and regulation. The main approaches to evaluating the business model of an enterprise are summarized. The principles of business model evaluation based on the principles of its innovativeness, adaptability and sustainability are defined. A system of evaluation methods and tools is formed, the conditions for their application, tasks and opportunities for making management decisions based on the evaluation are identified. A comparative assessment of the features of the formation of generations X, Y, Z, Alpha, events that formed these generations was carried out; their values, attitude to work, desire for feedback. It is noted that the theory of generations plays an important role in understanding these features and forming strategies for managing the development of human capital, since each generation has its own values, beliefs and approaches to work and learning. The issue of building an effective personnel motivation system at the enterprise is under consideration. The essence and advantages of implementing the policy of diversity and inclusion at modern enterprises are considered. It was emphasized that compliance with the policy of personnel diversity and inclusion of the workplace will allow attracting and retaining the necessary employees with high motivational readiness at the enterprise. The examines and analyzes contemporary challenges facing enterprises in a dynamic business environment, particularly in the context of the necessity for digital transformation and the formation of digital intelligence to ensure competitiveness. Proposed practical recommendations for organizations on the effective implementation of digital innovations in strategic management and achieving competitive advantage in a dynamic business environment. The study results of the influence of ESG and sustainability ratings on the multinational banks' war response strategies based on the Yale CELI database of companies leaving and staying in Russia are presented. It presents various aspects of sustainable development management in the context of modern management. The impact of globalization on the process of managing sustainable development and the role of public administration in this context are also considered. Additionally, the integration of sustainability concepts into local management practices and ethical aspects related to sustainability management are highlighted. Substantiates the importance of transition to an adaptive approach in the state regulation of investment activity and increase of the country's investment attractiveness in modern conditions. It has been established that adaptive regulation contributes to the achievement of stability and efficiency in the economic system, rapid response to external and internal challenges. The advisability of improving the methodologies of ESG rating providers is substantiated. The special importance of the leadership institute for the development of ecosocial management was revealed and, as a result, an analysis of the activities of the most successful innovative leaders of today was carried out, the result of which is the sustainable development and successful implementation of ecosocial management mechanisms at modern enterprises. It is established that today digital transformation is the use of digital technologies as a tool for reengineering business processes in higher education institutions. The main problematic aspects of the digitalization of education include the problem of adopting innovations, increasing the additional workload of teachers, shifting the vector of pedagogical work, and digital inequality. The challenges arising in the higher education system due to the development of artificial intelligence are identified in the results of scientific research. Artificial intelligence is revealed to be an important enabling mechanism for expanding teaching and learning opportunities, administering the educational process, and enhancing research potential. Higher education institutions can harness the transformative potential of artificial intelligence by using it responsibly and effectively. Based on a comprehensive analysis of real-life examples and research, the opportunities and challenges posed by MOOCs are discussed, as well as their implications for the future of quality provision in higher education.

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Частини книг з теми "Real-time cell analysis"

Haim-Vilmovsky, Liora. "High-Throughput Single-Cell Real-Time Quantitative PCR Analysis." In Methods in Molecular Biology, 177–83. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9240-9_11.

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Sklar, L. A., W. N. Swann, S. P. Fay, and Z. G. Oades. "Real-Time Analysis of Macromolecular Assembly During Cell Activation." In Biology of Cellular Transducing Signals, 1–10. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0559-0_1.

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Ish-Shalom, Shahar, and Amnon Lichter. "Analysis of Fungal Gene Expression by Real Time Quantitative PCR." In Molecular and Cell Biology Methods for Fungi, 103–14. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-611-5_7.

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Goudar, Chetan, Richard Biener, Chun Zhang, James Michaels, James Piret, and Konstantin Konstantinov. "Towards Industrial Application of Quasi Real-Time Metabolic Flux Analysis for Mammalian Cell Culture." In Cell Culture Engineering, 99–118. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/10_020.

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Edvardsson, Louise, and Tor Olofsson. "Real-Time PCR Analysis for Blood Cell Lineage Specific Markers." In DNA and RNA Profiling in Human Blood, 313–22. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-553-4_21.

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Ong, Seow Theng, and Navin Kumar Verma. "Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time." In Methods in Molecular Biology, 33–40. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9036-8_5.

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Jacobi, Angela, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, and Jochen Guck. "Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry." In Stem Cell Mobilization, 135–48. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9574-5_11.

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Bye, Alexander P., Zeki Ilkan, Amanda J. Unsworth, and Chris I. Jones. "Immobilization of Nonactivated Unfixed Platelets for Real-Time Single-Cell Analysis." In Methods in Molecular Biology, 1–11. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8585-2_1.

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Shulman, Ziv, and Ronen Alon. "Real-Time Analysis of Integrin-Dependent Transendothelial Migration and Integrin-Independent Interstitial Motility of Leukocytes." In Integrin and Cell Adhesion Molecules, 31–45. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-166-6_3.

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Brouwer, Ineke, Marit A. C. de Kort, and Tineke L. Lenstra. "Measuring Transcription Dynamics of Individual Genes Inside Living Cells." In Single Molecule Analysis, 235–65. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3377-9_12.

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AbstractTranscription is a highly dynamic process, which, for many genes, occurs in stochastic bursts. Studying what regulates these stochastic bursts requires visualization and quantification of transcription dynamics in single living cells. Such measurements of bursting can be accomplished by labeling nascent transcripts of single genes fluorescently with the MS2 and PP7 RNA labeling techniques. Live-cell single-molecule microscopy of transcription in real time allows for the extraction of transcriptional bursting kinetics inside single cells. This chapter describes how to set up the MS2 or PP7 RNA labeling system of endogenous genes in both budding yeast (Saccharomyces cerevisiae) and mammalian cells (mouse embryonic stem cells). We include how to genetically engineer the cells with the MS2 and PP7 system, describe how to perform the live-microscopy experiments and discuss how to extract transcriptional bursting parameters of the genes of interest.

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Тези доповідей конференцій з теми "Real-time cell analysis"

Roper, Carissa J., Chu Ma, and Susan C. Hagness. "Analysis of a TEM Cell Designed for Real-Time Observation of Pulsed Microwave-Induced Thermoelastic Tissue Expansion." In 2024 IEEE International Symposium on Antennas and Propagation and INC/USNC‐URSI Radio Science Meeting (AP-S/INC-USNC-URSI), 2515–16. IEEE, 2024. http://dx.doi.org/10.1109/ap-s/inc-usnc-ursi52054.2024.10686786.

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Wei, Yuanyuan, Syeda Aimen Abbasi, Meiai Lin, Liwei Tan, Shanhang Luo, Yuankai Ma, Shiyue Liu, Yi-Ping Ho, Ho-Pui Ho, and Wu Yuan. "Efficient Single-Cell Analysis through a Cost-Effective Droplet Microfluidic System." In CLEO: Applications and Technology, ATu3B.7. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_at.2024.atu3b.7.

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In this study, we introduce a cost-effective and high-throughput microfluidic system capable of generating uniform droplet reactors and conducting real-time analysis of single-cell morphology and apoptosis. This system holds great promise in the fields of biological research and personalized disease treatment.

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Sun, Jiasong, Yefeng Shu, and Chao Zuo. "Adaptive Optical Quantitative Phase Imaging of Living Cells Based on Fourier Ptychographic Microscopy." In Adaptive Optics: Methods, Analysis and Applications, OW1F.3. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/aopt.2024.ow1f.3.

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In this report, we present an adaptive optical Quantitative Phase Imaging (QPI) method based on annular illumination Fourier Ptychographic Microscopy (FPM). Using only six low-resolution images captured at six different illumination angles that match the objective, we're able to recover high-resolution quantitative phase images and characterize the aberrations in real time.

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Noubir, Safouane, Yannick Bornat, and Bertrand Le Gal. "Real-Time Analysis of Living Biological Cell Activity." In 2018 Conference on Design and Architectures for Signal and Image Processing (DASIP). IEEE, 2018. http://dx.doi.org/10.1109/dasip.2018.8596862.

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Xu, Wenkui, Liguo Chen, Haibo Huang, Leilei Zhang, Xiangpeng Li, Yadi Li, and Lining Sun. "Biomechanical analysis of yeast cell based a piezoresistive cantilever sensor." In 2016 IEEE International Conference on Real-time Computing and Robotics (RCAR). IEEE, 2016. http://dx.doi.org/10.1109/rcar.2016.7784058.

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Aoyama, Tadayoshi, Amalka De Zoysa, Qingyi Gu, Takeshi Takaki, and Idaku Ishii. "Real-time flow-rate control system for cell analysis." In the 2015 Conference. New York, New York, USA: ACM Press, 2015. http://dx.doi.org/10.1145/2783449.2783499.

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Schrimpf, J., M. Lind, and G. Mathisen. "Real-time analysis of a multi-robot sewing cell." In 2013 IEEE International Conference on Industrial Technology (ICIT 2013). IEEE, 2013. http://dx.doi.org/10.1109/icit.2013.6505666.

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Mota, Sakina Mohammed, Carl A. Gregory, Kristen C. Maitland, Maryellen L. Giger, Roland R. Kaunas, Robert E. Rogers, Andrew W. Haskell, and Eoin P. McNeill. "Morphological cell image analysis for real-time monitoring of stem cell culture." In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, edited by Thomas G. Brown and Tony Wilson. SPIE, 2019. http://dx.doi.org/10.1117/12.2507469.

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Fregin, Bob, Doreen Biedenweg, Salvatore Girardo, Stefan Groß, Konstanze Aurich, and Oliver Otto. "Dynamic real-time deformability cytometry: Time-resolved mechanical single cell analysis at 100 cells/s." In High-Speed Biomedical Imaging and Spectroscopy VII, edited by Keisuke Goda and Kevin K. Tsia. SPIE, 2022. http://dx.doi.org/10.1117/12.2624175.

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Park, Jonghan, and Soonhoi Ha. "Performance Analysis of Parallel Execution of H.264 Encoder on the Cell Processor." In 2007 IEEE/ACM/IFIP Workshop on Embedded Systems for Real-Time Multimedia. IEEE, 2007. http://dx.doi.org/10.1109/estmed.2007.4375797.

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Звіти організацій з теми "Real-time cell analysis"

Conery, Ian, Brittany Bruder, Connor Geis, Jessamin Straub, Nicholas Spore, and Katherine Brodie. Applicability of CoastSnap, a crowd-sourced coastal monitoring approach for US Army Corps of Engineers district use. Engineer Research and Development Center (U.S.), September 2023. http://dx.doi.org/10.21079/11681/47568.

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This US Army Engineer Research and Development Center, Coastal and Hydraulics Laboratory, technical report details the pilot deployment, accuracy evaluation, and best practices of the citizen-science, coastal-image monitoring program CoastSnap. Despite the need for regular observational data, many coastlines are monitored infrequently due to cost and personnel, and this cell phone-image-based approach represents a new potential data source to districts in addition to providing an outreach opportunity for the public. Requiring minimal hardware and signage, the system is simple to install but requires user-image processing. Analysis shows the CoastSnap-derived shorelines compare well to real-time kinematic and lidar-derived shorelines during low-to-moderate wave conditions (root mean square errors [RMSEs] <10 m). During high-wave conditions, errors are higher (RMSE up to 18 m) but are improved when incorporating wave run-up. Beyond shoreline quantification, images provide other qualitative information such as storm-impact characteristics and timing of the formation of beach scarps. Ultimately, the citizen-science tool is a viable low-cost option to districts for monitoring shorelines and tracking the evolution of coastal projects such as beach nourishments.

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Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

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The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.

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Delwiche, Michael, Boaz Zion, Robert BonDurant, Judith Rishpon, Ephraim Maltz, and Miriam Rosenberg. Biosensors for On-Line Measurement of Reproductive Hormones and Milk Proteins to Improve Dairy Herd Management. United States Department of Agriculture, February 2001. http://dx.doi.org/10.32747/2001.7573998.bard.

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The original objectives of this research project were to: (1) develop immunoassays, photometric sensors, and electrochemical sensors for real-time measurement of progesterone and estradiol in milk, (2) develop biosensors for measurement of caseins in milk, and (3) integrate and adapt these sensor technologies to create an automated electronic sensing system for operation in dairy parlors during milking. The overall direction of research was not changed, although the work was expanded to include other milk components such as urea and lactose. A second generation biosensor for on-line measurement of bovine progesterone was designed and tested. Anti-progesterone antibody was coated on small disks of nitrocellulose membrane, which were inserted in the reaction chamber prior to testing, and a real-time assay was developed. The biosensor was designed using micropumps and valves under computer control, and assayed fluid volumes on the order of 1 ml. An automated sampler was designed to draw a test volume of milk from the long milk tube using a 4-way pinch valve. The system could execute a measurement cycle in about 10 min. Progesterone could be measured at concentrations low enough to distinguish luteal-phase from follicular-phase cows. The potential of the sensor to detect actual ovulatory events was compared with standard methods of estrus detection, including human observation and an activity monitor. The biosensor correctly identified all ovulatory events during its testperiod, but the variability at low progesterone concentrations triggered some false positives. Direct on-line measurement and intelligent interpretation of reproductive hormone profiles offers the potential for substantial improvement in reproductive management. A simple potentiometric method for measurement of milk protein was developed and tested. The method was based on the fact that proteins bind iodine. When proteins are added to a solution of the redox couple iodine/iodide (I-I2), the concentration of free iodine is changed and, as a consequence, the potential between two electrodes immersed in the solution is changed. The method worked well with analytical casein solutions and accurately measured concentrations of analytical caseins added to fresh milk. When tested with actual milk samples, the correlation between the sensor readings and the reference lab results (of both total proteins and casein content) was inferior to that of analytical casein. A number of different technologies were explored for the analysis of milk urea, and a manometric technique was selected for the final design. In the new sensor, urea in the sample was hydrolyzed to ammonium and carbonate by the enzyme urease, and subsequent shaking of the sample with citric acid in a sealed cell allowed urea to be estimated as a change in partial pressure of carbon dioxide. The pressure change in the cell was measured with a miniature piezoresistive pressure sensor, and effects of background dissolved gases and vapor pressures were corrected for by repeating the measurement of pressure developed in the sample without the addition of urease. Results were accurate in the physiological range of milk, the assay was faster than the typical milking period, and no toxic reagents were required. A sampling device was designed and built to passively draw milk from the long milk tube in the parlor. An electrochemical sensor for lactose was developed starting with a three-cascaded-enzyme sensor, evolving into two enzymes and CO2[Fe (CN)6] as a mediator, and then into a microflow injection system using poly-osmium modified screen-printed electrodes. The sensor was designed to serve multiple milking positions, using a manifold valve, a sampling valve, and two pumps. Disposable screen-printed electrodes with enzymatic membranes were used. The sensor was optimized for electrode coating components, flow rate, pH, and sample size, and the results correlated well (r2= 0.967) with known lactose concentrations.

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WANG, MIN, Sheng Chen, Changqing Zhong, Tao Zhang, Yongxing Xu, Hongyuan Guo, Xiaoying Wang, Shuai Zhang, Yan Chen, and Lianyong Li. Diagnosis using artificial intelligence based on the endocytoscopic observation of the gastrointestinal tumours: a systematic review and meta-analysis. InPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, February 2023. http://dx.doi.org/10.37766/inplasy2023.2.0096.

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Review question / Objective: With the development of endoscopic techniques, several diagnostic endoscopy methods are available for the diagnosis of malignant lesions, including magnified pigmented endoscopy and narrow band imaging (NBI).The main goal of endoscopy is to achieve the real-time diagnostic evaluation of the tissue, allowing an accurate assessment comparable to histopathological diagnosis based on structural and cellular heterogeneity to significantly improve the diagnostic rate for cancerous tissues. Endocytoscopy (ECS) is based on ultrahigh magnification endoscopy and has been applied to endoscopy to achieve microscopic observation of gastrointestinal (GI) cells through tissue staining, thus allowing the differentiation of cancerous and noncancerous tissues in real time.To date, ECS observation has been applied to the diagnosis of oesophageal, gastric and colorectal tumours and has shown high sensitivity and specificity.Despite the highly accurate diagnostic capability of this method, the interpretation of the results is highly dependent on the operator's skill level, and it is difficult to train all endoscopists to master all methods quickly. Artificial intelligence (AI)-assisted diagnostic systems have been widely recognized for their high sensitivity and specificity in the diagnosis of GI tumours under general endoscopy. Few studies have explored on ECS for endoscopic tumour identification, and even fewer have explored ECS-based AI in the endoscopic identification of GI tumours, all of which have reached different conclusions. Therefore, we aimed to investigate the value of ECS-based AI in detecting GI tumour to provide evidence for its clinical application.

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Morrison, Dawn. The built environment of the US Air Force all-volunteer force : preliminary analysis of building trends. Engineer Research and Development Center (U.S.), September 2024. http://dx.doi.org/10.21079/11681/49360.

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July 1, 2023, marks the 50th anniversary of the beginning of the all-volunteer force (AVF). At this time, buildings, structures, and other elements of the US Air Force’s (USAF) built environment associated specifically with the AVF will be potentially eligible as historic resources under the National Historic Preservation Act (NHPA). The relationship between the AVF and the USAF built environment, however, has not yet been examined, and no historic contexts exist that provide guidance on how to identify and evaluate properties that may be associated with the built environment of the USAF AVF and offer recommendations on management of these properties to assist USAF installations in complying with the NHPA. As a result, it is unclear if, and to what extent, buildings, structures, and other elements associated specifically with the AVF exist that may require management under NHPA. The USAF desires to better understand the relationship between the AVF and the USAF built environment and has requested the Engineer Research and Development Center, Construction Engineering Research Laboratory (ERDC-CERL) conduct a built-environment analysis of existing USAF real property. This research is intended to support USAF decision-makers in determining if further research is warranted and how best to plan for managing AVF-related buildings, structures, and other built environment elements under NHPA. Results of this analysis indicate a relationship exists between the AVF and the USAF built environment; 42 built-environment feature types with construction rates higher than the overall average during the AVF period are identified.

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