Дисертації з теми "RAG1 expression"
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Braga, Aécio Assunção. "Polimorfismos dos genes CD40, ICAM-1, VCAM, E-selectina, LIGHT, RAGE e CX3CR1 relacionados com inflamação e sua associação à obesidade." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-26052015-143307/.
Повний текст джерелаBackground: Obesity is a serious health problem and it is defined as an excessive fat accumulation which is caused by an imbalance between the amount of energy intake and energy expenditure over a long period. Objective: The main objective of this study was to investigate the contribution of CD403 ICAM-1, VCAM, E-selectin, LIGHT, RAGE e CX3CR1 gene polymorphisms and its association with obesity. Material and Methods: The study was realized at Dante Pazzanese Institute of Cardiology and University Hospital of São Paulo University. There were included 199 individuals (40 normal weight, 55 overweight and 104 obese), all Brazilian, with no genetic link, from all ethnics in both genders (55 men and 144 women), aged between 30 and 68 years. The study of CD40 (rs1883832) C>T, CX3CR1 (rs3732379) C>T, CX3CR1 (rs3732378) G>A, E-selectin (rs5368) C>T, ICAM-1 (rs1799969) G>A , ICAM-1 (rs281432) C>G, LIGHT (rs344560) G>A, LIGHT (rs2291668) C>T, RAGE (rs2070600) G>A, RAGE (rs2236493) C>T and VCAM (rs3176878) C>T were conducted by pyrosequencing. The analysis of LIGHT, CX3CR1, RAGE and ICAM-1 gene expression were performed by real-time PCR and the measurements of PAI-1, IL- 6, TNF-a, resistin, leptin and adiponectin soluble forms using LUMINEX system. Results: The allele and genotype frequency of CD40 (rs1883832) C>T, CX3CR1 (rs3732379) C>T, CX3CR1 (rs3732378) G>A, E-selectin (rs5368) C>T, ICAM-1 (rs1799969) G>A , ICAM-1 (rs281432) C>G, LIGHT (rs344560) G>A, LIGHT (rs2291668) C>T, RAGE (rs2070600) G>A, RAGE (rs2236493) C>T and VCAM (rs3176878) C>T gene polymorphisms showed no significant association with obesity. There was found association in the analysis of CX3CR1 expression with obesity and it was found association of ICAM-1 gene polymorphism (rs281432) G>C with RAGE gene expression in the normal weight group. Conclusion: There was no association of CD40 (rs1883832) C>T, CX3CR1 (rs3732379) C>T, CX3CR1 (rs3732378) G>A, E-selectin (rs5368) C>T, ICAM-1 (rs1799969) G>A , ICAM-1 (rs281432) C>G, LIGHT (rs344560) G>A, LIGHT (rs2291668) C>T, RAGE (rs2070600) G>A, RAGE (rs2236493) C>T and VCAM (rs3176878) C>T gene polymorphisms with obesity. However, it was found association of CX3CR1 gene expression with obesity and an association of ICAM-1 (rs281432) G>C gene polymorphism with RAGE gene expression in normal weight individuals.
Lovejoy, Elizabeth A. "RAGE-based strategies for the control of gene expression." Thesis, University of Edinburgh, 1999. http://hdl.handle.net/1842/26699.
Повний текст джерелаCarvalho, Vanessa Isabel Dias Ribeiro de. "C. albicans Cek1 and Ras1: cloning, expression and purification." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/9526.
Повний текст джерелаCandida albicans é um fungo polimórfico e patogénico que reside de forma comensal nas superfícies mucosas humanas. Este fungo apresenta um código genético ambíguo, uma vez que, o codão universal leucina CUG é predominantemente traduzido como serina (97%), mas também como leucina (3%). A análise de proteínas de C. albicans que contêm resíduos CUG em importantes posições funcionais, revela que a ambiguidade do codão modela a função da proteína e poderá ter um papel determinante nas vias de sinalização associadas a mudanças morfológicas e patogénicas. Com o presente estudo pretende-se investigar o efeito da leucina e da serina nas posições CUG (ambiguidade CUG) na estrutura e função de duas proteínas chave nas vias de sinalização de C. albicans, a Ras1 (GTPase) e a Cek1 (Cinase). Estas regulam a transcrição dos genes associados com mudanças morfológicas, patológicas e, por outro lado, contêm resíduos CUG numa posição estritamente conservada e com relevo funcional. Neste contexto foi possível clonar com sucesso genes sintéticos para os centros ativos da Ras1 e Cek1 (variantes de serina e de leucina para o codão CUG) em vetores que apresentam diferentes caudas de solubilidade (MBP, NusA, Trx, ZTag2 e Gb1). Foram desenvolvidos protocolos de alta expressão bacteriana e de purificação para os domínios ativos Ras1 (ligado à Gb1) e Cek1 (ligado à MBP). A análise dos resultados de purificação analítica e de “Dynamic Light Scaterring” demonstraram que as proteínas recombinantes se apresentam na forma monomérica. Ensaios de cristalização estão a ser realizados esperando-se determinar as estruturas tridimensionais das proteínas por cristalografia de raio-X. As estruturas da Cek1 e Ras1 com leucina e serina nas posições CUG, conjuntamente com uma análise meticulosa da sua estabilidade e função in vitro, irão fornecer informações importantes sobre o papel estratégico da ambiguidade natural do codão.
The polymorphic fungal pathogen Candida albicans has an ambiguous genetic code, as the universal leucine CUG codon is predominantly translated as serine (97%) but also as leucine (3%). Analysis of the rare C. albicans proteins containing CUG-encoded residues in functionally relevant positions reveals that codon ambiguity shapes protein function and might have a pivotal role in signaling cascades associated with morphological changes and pathogenesis. The present study investigates the effect of leucine or serine at CUG positions (CUG ambiguity) in the structure and function of two key effectors of signaling cascades in C. albicans, Ras1 (GTPase) and Cek1 (protein kinase), which regulate the transcription of genes associated with morphological changes and pathogenesis. These two proteins contain a CUG residue in a strictly conserved and functionally relevant position. Synthetic genes coding for the active domains of Ras1 and Cek1 (serine and leucine variants for the CUG codon) were successfully cloned into expression vectors carrying different solubility partners (MBP, NusA, Trx, ZTag2 and Gb1). Furthermore, using an incomplete factorial approach, high level bacterial expression and purification protocols for the active domains of Ras1 (in fusion with Gb1) and Cek1 (in fusion with MBP) were developed. Analytical size exclusion chromatography (SEC) and dynamic light scattering (DLS) results indicate that both recombinant proteins are monomeric. Crystallization trials must be done aiming for the determination of their threedimensional structures by X-ray crystallography. The structures of Ras1 and Cek1 with serine or leucine at CUG positions, together with a thorough analysis of their stability and function in vitro, will provide valuable insights into a possible strategic role of natural codon ambiguity.
Kim, Sun. "HAPLOINSUFFICIENCY OF RAI1 AND ITS EFFECT ON BDNF EXPRESSION." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/165.
Повний текст джерелаChen, Suzi Su-Hsin, and suzi chen@med monash edu au. "Cyclooxygenase Expression in Human Diabetes." RMIT University. Medical Sciences, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080206.121439.
Повний текст джерелаSikora, Kristin [Verfasser]. "RAGE-abhängige S100A8- und S100A9-Expression in humanen THP-1 Zellen / Kristin Sikora." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2009. http://d-nb.info/1023749920/34.
Повний текст джерелаGonçalves, Carolina de Souza. "Expressão de proteínas RAP1 recombinantes e produção de anticorpos anti- RAP1: potencial uso como biomarcador no diagnóstico de tumores." s.n, 2014. https://www.arca.fiocruz.br/handle/icict/9945.
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Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Alterações imunofenotípicas qualitativas e quantitativas na expressão da proteína RAP1, uma pequena GTPase da superfamília RAS, estão presentes em diversos tipos de cânceres, tais como carcinomas de células escamosas de orofaringe, câncer papilar da tireóide, câncer de mama, carcinoma de células renais, leucemia, melanoma, neoplasias intraepiteliais e câncer cervical. Entretanto, para a utilização de RAP1 como biomarcador para auxiliar no diagnóstico imuno-histoquímico de tumores, especialmente do câncer cervical, são necessários anticorpos anti-RAP1 a baixo custo, uma vez que, atualmente, os anticorpos disponíveis no Brasil são importados e de custo elevado, tornando inviável sua utilização no diagnóstico de rotina. Assim, este trabalho tem como objetivos a expressão de proteínas RAP1 recombinantes (rRAP1) em sistema bacteriano, e a produção de anticorpos monoclonais e policlonais anti-rRAP1, visando a sua aplicação no diagnóstico de diversos tumores por imuno-histoquímica. Dois genes RAP1 sintéticos codificantes para as proteínas rRAP1A e rRAP1AB foram desenhados, sintetizados e subclonados no plasmídeo de expressão bacteriano pQE9 e sua expressões obtidas na linhagem hospedeira E.coli M15. Após indução com IPTG, as proteínas rRAP1 foram purificadas por cromatografia líquida em coluna de afinidade de quelato de níquel, obtendo-se o rendimento, por litro de cultura bacteriana, de 185,6 mg/L de rRAP1A e 103,9 mg/L de rRAP1AB. As proteínas rRAP1 purificadas foram inoculadas em animais para a produção de anticorpos monoclonais e policlonais anti-rRAP1A e antirRAP1AB. Ensaios imuno-histoquímicos foram realizados em tecidos de pacientes com neoplasia cervical para a avaliação da reatividade dos anticorpos anti-rRAP1 com a proteína RAP1 humana. Uma intensa imunorreatividade foi verificada com o anticorpo anti-rRAP1A (policlonal produzido em coelhos) considerado, até o momento, o melhor candidato para uso na detecção da expressão de RAP1 em ensaios imuno-histoquímicos, o que pode auxiliar no diagnóstico de várias neoplasias, especialmente, do câncer do colo uterino.
Qualitative and quantitative immunophenotypical changes in the expression of RAP1 protein, a small GTPase of the RAS superfamily, have been detected in many types of cancers such as oropharyngeal squamous cell carcinomas, papillary thyroid cancer, breast cancer, renal cell carcinoma, leukemia, melanoma, intraepithelial neoplasia and cervical cancer. However, to use RAP1 as a putative biomarker for immunohistochemical assays to support tumor diagnosis, especially cervical cancer, anti-RAP1 antibodies at low cost are essential, since here in Brazil the anti-RAP antibodies available are imported and expensive making them impractical to use in routine diagnostics. This work aims to express recombinant proteins RAP1 (rRAP1) in bacterial system for the production of monoclonal and polyclonal anti-rRAP1 to be used for diagnosis of various tumors by immunohistochemistry. Two synthetic RAP1 genes coding for rRAP1A and rRAP1AB proteins were designed, synthesized, and subcloned into the pQE9 vector for recombinant protein production in E. coli (strain M15). After IPTG induction, both rRAP1 proteins were purified by nickel chelate affinity chromatography yielding, per liter of bacterial culture, 185,6 mg/L of rRAP1A and 103,9 mg/L of rRAP1AB protein. The purified rRAP1 proteins were used to generate polyclonal and monoclonal antibodies against rRAP1A and against rRAP1AB. Immunohistochemistry experiments were performed on tissues samples from patients with cervical neoplasia to evaluate the reactivity of the anti-rRAP1 antibodies to the human RAP1. An intense immunoreactivity was observed for a rabbit polyclonal anti-rRAP1A antibody, considered so far, the best candidate to be used for RAP1 immunohistochemical testing to support tumor diagnosis, especially cervical cancer.
Di, Candia Leonarda. "The expression and function of RAGE and HMGB1 in airway structural cells in asthma." Thesis, University of Leicester, 2015. http://hdl.handle.net/2381/32339.
Повний текст джерелаLalk, Michael [Verfasser]. "Tumorregionen-abhängige Expression der Aminosäure-Sensoren MAP4K3, RagC und VPS34 in Glioblastomen / Michael Lalk." Magdeburg : Universitätsbibliothek, 2018. http://d-nb.info/1174626593/34.
Повний текст джерелаRösch, Daniela. "Regulation der Expression der Rezeptoren für advanced glycation end products (RAGE) auf humanen Monozyten." [S.l. : s.n.], 2006.
Знайти повний текст джерелаGillis, L. Jane. "Expression and recombinase activity of RAG 1 and two splice variants of RAG 2 in mature human primary tonsilar B lymphocytes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0004/MQ46022.pdf.
Повний текст джерелаFerhani, Nassima. "Expression pulmonaire et rôle fonctionnel d'HMGB1 et de son recepteur, RAGE, dans la bronchopneumopathie chronique obstructive." Paris 7, 2010. http://www.theses.fr/2010PA077265.
Повний текст джерелаCOPD is characterized by airway inflammation and remodeling. HMGBl, a nuclear protein that is released during inflammation and repair, interacts with pro-inflammatory cytokines and with its receptor, RAGE, which is highly expressed in the lung. In the present study, we have shown higher levels of HMGB 1 in bronchoalveolar lavage (BAL) from smokers with COPD, as compared to smokers and never smokers, and similar differences wer observed in epithelial cells and alveolar macrophages. BAL HMGBl correlated positively with the levels of pro-inflammatory mediators in BAI including IL-1D, and with the degree of airflow obstruction and emphysema. HMGBl-IL-1D D complexes were found in BAL supernatant and alveolar macrophages from smokers and COPD patients, as well as in the human macrophage cell line, THP-1, where they enhanced the synthesis of TNF-C RAGE was overexpressed in the airway epithelium and smooth muscle of COPD patients and it co-localized with HMGBl. Finally, in preliminary experiments we demonstrated that HMGBl delays epithelium repair in an in vitro model of mechanical wound injury using human bronchial epithelial cells growth in a air-liquid interface conditions. We conclude that elevated HMGBl expression in COPD airways may sustain inflammation through i interaction with IL-1D and RAGE and may contribute to airway remodeling by interfering with the normal epithelial repair process. Therefore strategies aimed at inhibiting the expression of HMGBl and RAGE or at blocking their interaction in target cells would be of therapeutic value f( attenuating lung remodelling and the accompanying respiratory functional deterioration in COPD
Alexander, Kristen Lena. "Differential Receptors for Advanced Glycation End-Products (RAGE) Expression in Preeclampsia, Intrauterine Growth Restriction and Gestational Diabetes." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5463.
Повний текст джерелаSilva, Marcela Claudino da. "Análise da expressão de citocinas e dos receptores RAGE no periodonto de ratos diabéticos." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/25/25149/tde-02062011-150539/.
Повний текст джерелаPeriodontal diseases (PD) are chronic inflammatory diseases leading the destruction of connective tissue and alveolar bone supporting the teeth. The establishment of diabetes increases PD prevalence and severity in humans and experimental model. However, biological mechanisms regarding to increase of prevalence and severity remains poorly known. The aim of this study was to evaluate the number of immuno-staining cells to TNF- IL-1 , IL-6, MMP-2, MMP-9, RANKL and RAGE receptors in experimental periodontal disease in diabetic rats. Diabetes was induced in Wistar rats (n=25) by endovenous administration of 42 mg/kg of alloxan, and together with control animals (n=25), were analyzed at 1, 3, 6, 9 and 12 months after diabetes induction. The animals were sacrificed and the jaws were removed and submitted to immunohistochemistry procedures. Our data demonstrated that diabetes induction and progression resulted in significant alterations in number of immuno-staining cells to different mediators of inflammatory process. In diabetic rats, we observed an increased number of immuno-staining cells to TNF- (6 and 12 months), IL-1 (12 months), IL-6 (9 e 12 months), RANKL (9 months) and RAGE receptors (6, 9 and 12 months) (p<0,05 ANOVA). Regarding to MMP-2+ and MMP-9+ cells, we did not found differences between control and experimental groups. However, we found a trend of towards in MMP-9+ cells in diabetic rats after 12 months of diabetes induction (p>0,05 ANOVA). Then, our data demonstrated that diabetes establishment and progression resulted in an increase of immuno-staining cells to TNF- , IL-1 IL-6, RANKL and RAGE receptors. Taken together, desregulation of inflammatory cytokines and osteoclastogenic factor expression seems to be one of biological mechanisms involved in the increase of periodontal disease prevalence and severity associated with diabetes.
Hausherr, Carolin Kim. "Konditionale Expression von HER-2, H-Ras oder BXB-Raf1 in einem Maustumormodell molekuare [molekulare] Mechanismen der Tumorremission /." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975920693.
Повний текст джерелаChavez, Matias Elizabeth Murayama. "Expression of Osteoarthritis Biomarkers in Temporomandibular Joints of Mice with and Without Receptor for Advanced Glycation End Products (RAGE)." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5242.
Повний текст джерелаEjdesjö, Andreas. "Teratogenic Predisposition in Diabetic Rat Pregnancy." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-178175.
Повний текст джерелаSawafta, Ashraf. "Design of vector for the expression of shRNA in transgenic animals." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2008. http://tel.archives-ouvertes.fr/tel-00812776.
Повний текст джерелаMaxwell, Morgan. "Rage and social media: The effect of social media on perceptions of racism, stress appraisal, and anger expression among young African American adults." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4311.
Повний текст джерелаTahiri-Alaoui, Abdessamad. "Modifications cellulaires et moléculaires après infection des racines de Nicotiana par le champignon pathogène Chalara elegans (Nag Rag & Ken. ) : mécanismes de défense et comparaison avec une infection symbiotique." Dijon, 1992. http://www.theses.fr/1992DIJOS035.
Повний текст джерелаFigueiredo, Anna Carolina Cançado. "Caracterização imunocitoquímica da expressão da proteína RAP1 em blocos de células escamosas provenientes de citologia cervical em meio líquido." s.n, 2015. https://www.arca.fiocruz.br/handle/icict/10915.
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Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou
O diagnóstico precoce acurado do câncer cervical, relevante problema de saúde pública no mundo e no Brasil, pela citologia oncótica (Teste de Papanicolaou), é muito prejudicado pela subjetividade e variabilidade dos resultados falsos negativos e falsos positivos do método, particularmente diante de células escamosas atípicas (ASC). Recentes inovações técnicas, como citologia em meio líquido e imunocitoquímica com biomarcadores de proliferação celular, aumentaram a expectativa de melhorias no rastreamento do câncer cervical. No entanto, a aplicabilidade destas inovações nos estágios mais iniciais de atipia e displasias epiteliais permanecem incertas. Assim, considerando resultado prévio do nosso grupo de pesquisa, que identificou a proteína RAP1 como biomarcador diagnóstico da displasia cervical, este trabalho tem como objetivo caracterizar a expressão da proteína RAP1, comparativamente à expressão dos biomarcadores p16 e Ki-67, por imunocitoquímica, em blocos de células escamosas cervicais para possível aplicabilidade na triagem do câncer do colo do útero. Para tal, 34 amostras, 27 pacientes com diagnóstico de alterações celulares benignas (ACB) e 7 pacientes com diagnóstico de ASC foram coletadas na unidade Jenny Faria do Hospital das Clínicas da UFMG. Os resultados indicaram que 85% das amostras de blocos celulares foram satisfatórias para análise morfológica e a técnica reproduziu os principais parâmetros citopatológicos da citologia convencional. Em relação a sua utilização para diagnóstico, o bloco de células apresentou sensibilidade de 38,46%, especificidade de 90,47% e uma variabilidade interobervador com taxa de concordância de aproximadamente 30% para os grupos ACB e ASC. A expressão da proteína RAP1 foi positiva na maioria das amostras do grupo “ACB” (15/27 ou 55,56%) e resultado negativo na maioria das amostras do grupo “ASC” (4/7 ou 57,14%) com sensibilidade de 16,66%, especificidade de 75, %. As reações imunocitoquímicas das proteínas p16 e Ki-67 demonstraram, em ambos os grupos, apresentaram predomínio absoluto ou totalidade de resultados negativos. O DNA de HPV foi detectado em 9 (33,33%) das 27 amostras do grupo ACB e em 4 (57,14%) das 7 amostras do grupo ASC. O HPV-16 foi detectado nas 4 amostras do grupo ASC. Nas amostras do grupo ACB foram detectados os HPV-16, em 5 amostras, HPV-58, em 2 amostras, HPV-45, em 1 amostra e HPV-66 em 1 amostra. Observamos inexistência de relação entre a presença do HPV e a expressão imunocitoquímica de RAP1 em ambos os grupos. Em conclusão, os blocos de células podem complementar o teste de Papanicolaou na triagem do câncer do colo uterino e a expressão da proteína RAP1 está aumentada em células cervicais em ambiente inflamatório, associado ou não à presença de HPV
The accurate early diagnosis of cervical cancer, relevant public health problem worldwide and in Brazil by cytology (Pap test), is hampered by its subjectivity and variability of false positive and false negative results, particularly on atypical squamous cell (ASC). Recent technical innovations, such as based liquid cytology and immunocytochemistry for with cell proliferation biomarkers, increased the expectation cervical cancer screening. However, the applicability of these innovations in early stages of epithelial dysplasia and atypia remains uncertain. Considering previous findings of our research group, which identified the RAP1 protein as a biomarker diagnosis of cervical dysplasia, this study aims to characterize the expression of RAP1 (compared to the expression of p16 and Ki-67 biomarkers) by immunocytochemistry in cell blocks of cervical squamous cells, for possible applicability in screening for cervical cancer. For this purpose, 27 patients with benign cellular changes (ACB) and 7 patients with ASC diagnosis were collected in Hospital das Clínicas from UFMG. The results indicated that 85% of the samples of cell blocks were satisfactory for morphological analysis and also that the cytological technique reproduces the main parameters of the conventional cytology. Regarding its use for diagnosis, the cell block had a sensitivity of 38.46%, specificity of 90.47% and an interobserver variability with concordance rate of approximately 30% for the ACB and ASC groups. The RAP1 expression was positive in most of the samples of the group "ACB" (15/27 or 55.56%) and most negative in the sample group "ASC" (4/7 or 57.14%) with a sensitivity of 16.66% and specificity of 75%. The immunocytochemical reactions for p16 and Ki-67 showed predominance of negative or all negative staining in both groups. HPV DNA was detected in 9 (33.33%) of the 27 samples of the ACB group and in 4 (57.14%) of 7 ASC group samples. HPV-16 was detected in 4 samples ASC group. Samples from the group ACB HPV-16 were detected in 5 samples, HPV-58 in 2 samples, HPV-45, HPV-1 sample, and 66 in one sample. We observed no relationship between the presence of HPV and immunostaining of RAP1 in both groups. In conclusion, cell blocks can be a ancillary tool to the Pap test for cervical cancer in screening and the expression of the RAP1 protein is increased in cervical cells in an inflammatory environment, with or without the presence of HPV.
Labrie, Joseph E. III. "Expression of rag2 and V(D)J Recombinase Activity are Reduced in Aged Mice as a Result of Changes in the Bone Marrow Microenvironment: a Dissertation." eScholarship@UMMS, 2004. https://escholarship.umassmed.edu/gsbs_diss/236.
Повний текст джерелаFantino, Emmanuelle. "Expression transcriptionnelle du gène SRP1 de Saccharomyces cerevisiae : caractérisation d'une séquence intragénique cis-activatrice et implication du facteur général de transcription TUF/RAP1/GRF1." Aix-Marseille 2, 1991. http://www.theses.fr/1991AIX22006.
Повний текст джерелаKanellopoulou, Chrysi. "Analysis of locus accessibility for V(D)J recombination and its potential in generating a mouse model for monitoring RAG protein expression in peripheral B cells." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=965433579.
Повний текст джерелаGravel, William-Édouard. "Expression de la dystrophine humaine dans le Tibialis anterior de souris Rag/mdx suite à une greffe de cellules myogéniques dérivées d'hiPSCs dystrophiques et corrigées génétiquement." Master's thesis, Université Laval, 2016. http://hdl.handle.net/20.500.11794/26698.
Повний текст джерелаHuman embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs) have shown self-renewal capacity and can potentially differentiate into all types of cell lineages. They represent an unlimited source of cells for the therapy of degenerative diseases, such as Duchenne Muscular Dystrophy (DMD), a disease characterized by a rapid degeneration of muscles that starts early in life. Dystrophic hiPSCs have been corrected by our collaborator, Dr. Hotta, by inserting of a single base pair in the exon 45 with Transcription Activator-Like Effector Nucleases (TALENs) to restore the reading frame of the gene. Our laboratory has developed a two-step procedure to differentiate hiPSCs into myogenic cells. We first used a myogenic culture medium especially developped in the laboratory (called MB-1) to promote the differentiation of hiPSCs into mesenchymal-like precursor cells. We next transduced them with a lentivirus expressing the myogenic transcription factor MyoD under the control of the composite CAG promoter, in order to induce their differentiation into myoblasts. Transduced cells have been grafted in the Tibialis anterior muscle of Rag/mdx mice where they fused with existing muscle fibers. The presence of the human dystrophin protein has been confirmed by immunohistofluorescence in muscles grafted with the genetically corrected cells and in a control graft with myoblasts of a healthy donor. Cell therapy shows great promises for DMD patients since it allows the expression of a normal gene capable of producing a functional dystrophin in muscle fibers and increase the regenerative capacity of the muscle and the muscle strength.
Degani, G. "MOLECULAR CHARACTERIZATION OF MEMBRANE-BOUND GLYCOPROTEINS INVOLVED IN HUMAN DISEASES AND POTENTIAL TARGETS FOR NEW THERAPIES." Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/274187.
Повний текст джерелаRosa, Júnior Nevton Teixeira da. "Determinação da correlação entre as proteínas do complexo shelterin, disquerina, citocinas inflamatórias e comprimento dos telômeros em indivíduos portadores de obesidade." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/157951.
Повний текст джерелаIn individuals with obesity, the excess of adipose tissue plays a key role in inducing a chronic and systemic inflammatory state. Like aging, obesity mimics cellular processes such as deterioration of tissues and organs and decreased ability to repair age-dependent DNA damages. In this context, the proinflammatory cytokines induce DNA damage that impact, especially in the most susceptible regions of the chromosomes, the telomeres. The telomeres, present at the ends of the chromosomes, are associated with a protein complex called the shelterin complex. The shelterin complex consists of 6 proteins (TRF1, TRF2, TIN2, POT1, TPP1 and RAP1), which together with accessory proteins such as dyskerin (DKC1), participate in telomere’s length regulation and protect chromosomes from undesired erosion, enzymatic activities, non-homologous recombination and fusion of chromosomal terminations. In recent years, positive relationships have been established between clinically different pathological conditions, such as those modulated by inflammation, and telomeres’ length. Recently, our group demonstrated shortened telomeres in individuals with morbid obesity. Thus, the aim of the present study was to explore additional factors associated with telomeres’ metabolism, such as gene expression of the shelterin complex components and proinflammatory cytokines, which may contribute to the accelerated shortening of the telomeres. We used peripheral blood mononuclear cells (PBMC) samples from healthy adults (n = 27) and adults with obesity (n = 39). We quantified gene expression by reverse transcription and quantitative PCR (RT-qPCR) of all shelterin complex genes, DKC1, IL-1β and TNF-α. Our results demonstrate an altered gene expression profile when compared to the gene expression of the proteins analyzed in the two studied groups, controls and individuals with obesity. Individuals with obesity showed a significantly elevated profile of TRF1, POT1, RAP1 and DKC1 (P < 0.05) genes. We did not observe correlation of gene expression between the different shelterin genes and the length of telomeres in the studied groups, but with DKC1 in obesity. However, when we analyzed the associations between the shelterin complex genes we observed significant changes in the intra-group associations dependent on the obesity condition. Our results highlight the complex and intricate network of associated and deregulated factors during the pathophysiological process of obesity. Further studies are needed together with the inclusion of additional factors to try to dissect the coordinated regulation of telomeres’ length in homeostasis and in the pathological process of obesity.
Muth, Ingrid Elisabeth Verfasser], and Mathias [Akademischer Betreuer] [Bähr. "Die Expression von High Mobility Group Box 1 (HMGB1) und dessen Receptor for Advanced Glycation Endproducts (RAGE) als Pathomechanismus der sporadischen Einschlusskörpermyositis / Ingrid Elisabeth Muth. Gutachter: Mathias Bähr. Betreuer: Mathias Bähr." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2009. http://d-nb.info/1043027270/34.
Повний текст джерелаWaern, Johan [Verfasser], and Michael [Akademischer Betreuer] Ott. "Effects of ectopic murine CD47 expression on human hepatocyte engraftment in Rag, gamma c uPA mice / Johan Martin Waern. Klinik für Gastroenterologie, Hepatologie und Endokrinologie der Medizinische Hochschule Hannover. Betreuer: Michael Ott." Hannover : Bibliothek der Medizinischen Hochschule Hannover, 2012. http://d-nb.info/1025784057/34.
Повний текст джерелаMedeiros, Marcell Costa de [UNESP]. "Modulação da proliferação, morte celular e expressão gênica de mediadores inflamatórios pela ativação de RAGE e TLR4 em células da resposta imune inata e adaptativa: papel das vias de sinalização p38 MAPK e NF-kB." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/96192.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O diabetes está associado à maior susceptibilidade à infecções e sepsis, demonstrando uma influência desta condição sobre a resposta imune. A doença periodontal é um tipo de infecção crônica, modulada pela resposta imune que apresenta maior prevalência e severidade em pacientes diabéticos. Nosso objetivo foi avaliar um possível sinergismo entre os receptores RAGE e TLR4 na modulação da proliferação celular, atividade metabólica, apoptose e expressão de citocinas inflamatórias em células da resposta imune inata e adaptativa. Como objetivo secundário, avaliamos o papel das vias de sinalização p38 MAPK e NF-kB na expressão dos genes inflamatórios por estas células após estimulação de RAGE e TLR4. Linhagens de células humanas de linfócitos T (JM) e monócitos (U937) foram estimulados com LPS e AGE-BSA tanto de forma independente como associados. A estimulação foi realizada também na presença e na ausência de inibidores bioquímicos para p38 MAPK (SB203580) e NF-kB (Bay 11-7082). A proliferação celular foi determinada por ensaio de exclusão azul de trypan, a apoptose pela via intrínseca e atividade metabólica foi avaliada por um ensaio bioquímico da função 18 mitocondrial, a expressão de citocinas foi estudada por RT-PCR e RT-qPCR e a ativação das vias de sinalização de interesse pelos estímulos utilizados foi investigada através de Western blotting. LPS e AGE-BSA não influenciaram a proliferação e sobrevivência celular de monócitos e linfócitos T após 24, 48 e 72 h. LPS, isoladamente ou associado a AGE, induziu a expressão de IL-6 e TNF-α em monócitos e células T, respectivamente. A ativação de p38 MAPK...
Diabetes is associated to increased susceptibility to infections and sepsis, indicating that this condition modulates the immune response. Periodontal disease is one type of chronic infection, which is modulated by the immune response and presents with increased prevalence and severity in diabetic patients. Our objective was to evaluate a possible synergism between RAGE and TLR4 signaling on the modulation of cell proliferation, metabolic activity, apoptosis and gene expression of inflammatory cytokines by cells of the innate and adaptive immune response. As a secondary objective, we assessed the role of p38 MAPK and NF-kB signaling pathways on the expression of the inflammatory genes by these cells after stimulation of RAGE and TLR4. Human cell lines of T lymphocytes (JM) and monocytes (U937) were stimulated with LPS and AGE-BSA both independently and associated. Stimulation was also performed in the presence and absence of biochemical inhibitors for p38 MAPK (SB203580) and NF-kB (Bay 11-7082). Cell proliferation was determined by trypan blue dye exclusion assay, apoptosis by the intrinsic pathway and metabolic activity were assessed by a biochemical assay of 21 the mitochondrial function, cytokine gene expression was studied by RT-PCR and RT-qPCR and the activation of the selected signaling pathways after RAGE and TLR4 activation was investigated by Western blotting. LPS and AGE-BSA did not influence cell proliferation and survival 24, 48 and 72 h after stimulation. LPS, alone or associated with AGE-BSA, induced expression of IL-6 and TNF- mRNA by monocytes and T cells, respectively. Activation of p38 MAPK, but not of NF-kB, was required for LPS and LPS/AGE-induced induction of IL-6 and TNF. RAGE mRNA expression was detected in both cell types. CCL3 mRNA expression levels were higher in monocytes upon... (Complete abstract click electronic access below)
Medeiros, Marcell Costa de. "Modulação da proliferação, morte celular e expressão gênica de mediadores inflamatórios pela ativação de RAGE e TLR4 em células da resposta imune inata e adaptativa : papel das vias de sinalização p38 MAPK e NF-kB /." Araraquara : [s.n.], 2012. http://hdl.handle.net/11449/96192.
Повний текст джерелаBanca: José Eduardo Cezar Sampaio
Banca: Adriana Campos Passanezi Sant'Ana
Resumo: O diabetes está associado à maior susceptibilidade à infecções e sepsis, demonstrando uma influência desta condição sobre a resposta imune. A doença periodontal é um tipo de infecção crônica, modulada pela resposta imune que apresenta maior prevalência e severidade em pacientes diabéticos. Nosso objetivo foi avaliar um possível sinergismo entre os receptores RAGE e TLR4 na modulação da proliferação celular, atividade metabólica, apoptose e expressão de citocinas inflamatórias em células da resposta imune inata e adaptativa. Como objetivo secundário, avaliamos o papel das vias de sinalização p38 MAPK e NF-kB na expressão dos genes inflamatórios por estas células após estimulação de RAGE e TLR4. Linhagens de células humanas de linfócitos T (JM) e monócitos (U937) foram estimulados com LPS e AGE-BSA tanto de forma independente como associados. A estimulação foi realizada também na presença e na ausência de inibidores bioquímicos para p38 MAPK (SB203580) e NF-kB (Bay 11-7082). A proliferação celular foi determinada por ensaio de exclusão azul de trypan, a apoptose pela via intrínseca e atividade metabólica foi avaliada por um ensaio bioquímico da função 18 mitocondrial, a expressão de citocinas foi estudada por RT-PCR e RT-qPCR e a ativação das vias de sinalização de interesse pelos estímulos utilizados foi investigada através de Western blotting. LPS e AGE-BSA não influenciaram a proliferação e sobrevivência celular de monócitos e linfócitos T após 24, 48 e 72 h. LPS, isoladamente ou associado a AGE, induziu a expressão de IL-6 e TNF-α em monócitos e células T, respectivamente. A ativação de p38 MAPK... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Diabetes is associated to increased susceptibility to infections and sepsis, indicating that this condition modulates the immune response. Periodontal disease is one type of chronic infection, which is modulated by the immune response and presents with increased prevalence and severity in diabetic patients. Our objective was to evaluate a possible synergism between RAGE and TLR4 signaling on the modulation of cell proliferation, metabolic activity, apoptosis and gene expression of inflammatory cytokines by cells of the innate and adaptive immune response. As a secondary objective, we assessed the role of p38 MAPK and NF-kB signaling pathways on the expression of the inflammatory genes by these cells after stimulation of RAGE and TLR4. Human cell lines of T lymphocytes (JM) and monocytes (U937) were stimulated with LPS and AGE-BSA both independently and associated. Stimulation was also performed in the presence and absence of biochemical inhibitors for p38 MAPK (SB203580) and NF-kB (Bay 11-7082). Cell proliferation was determined by trypan blue dye exclusion assay, apoptosis by the intrinsic pathway and metabolic activity were assessed by a biochemical assay of 21 the mitochondrial function, cytokine gene expression was studied by RT-PCR and RT-qPCR and the activation of the selected signaling pathways after RAGE and TLR4 activation was investigated by Western blotting. LPS and AGE-BSA did not influence cell proliferation and survival 24, 48 and 72 h after stimulation. LPS, alone or associated with AGE-BSA, induced expression of IL-6 and TNF- mRNA by monocytes and T cells, respectively. Activation of p38 MAPK, but not of NF-kB, was required for LPS and LPS/AGE-induced induction of IL-6 and TNF. RAGE mRNA expression was detected in both cell types. CCL3 mRNA expression levels were higher in monocytes upon... (Complete abstract click electronic access below)
Mestre
Karol, Sven. "Well-Formed and Scalable Invasive Software Composition." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-170162.
Повний текст джерелаNilavar, Namrata M. "Investigation of RAG1 in lymphoid and brain cells: Understanding mechanism and regulation." Thesis, 2020. https://etd.iisc.ac.in/handle/2005/5132.
Повний текст джерелаWang, Ya-Jean, and 王雅貞. "Cloning recombination activating gene 1 and 2 ( rag1 and rag2 ) and analyzing of the genes expression in adaptive immunity ontogenesis of Orange-Spotted Grouper (Epinephelus coioides)." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/38350825233940536103.
Повний текст джерела國立成功大學
生物學系碩博士班
97
Orange-spotted grouper (Epinephelus coioides) is a fish species with a high economic importance in the aquaculture industry in Taiwan. The high mortalities observed throughout early development such as viral nervous necrosis (VNN) causes the highest mortalities up to 100% always occur among 1-month-old larvae with total body lengths of 2.0 cm. Teleost is the oldest species has the adaptive immune system. However, there is a risk of inducing immunological tolerance if fish that are immunised at a very early age before they are immunocompetent. Thus, it is important to establish the earliest time that grouper can be vaccinated or give immunopotentiating agent. Recombination activating genes, rag1 and rag2, encode components of the recombinase involved in V(D)J recombination. During B lymphocyte development, the variable region of Ig gene is assembled by the recombination of multiple V, D, J segments, which can generate a vast array of immunoglobulin M (IgM) to against numerous antigen. IgM produced by B lymphocytes is also an important gene that can be used in the study of the ontogenesis of the immune system, as it is the first formed antibody of the primary humoral component of the acquired immune system in fish species. These genes are expressed together in this study in order to understand the expression profile of them. The genes, rag1 and rag2, of E. coioides were cloned and sequenced the open reading frames. The full-length cDNA of rag1 and rag2 were 3653 and 1875 base pairs (bp) long respectively. The lengths of rag1 and rag2 open reading frame were 3216 and 1602 bp encoding 1071 and 533 amino acids with the molecular weight of putative protein were about 117 and 58 kDa. Subsequently, the rag1, rag2 and IgM mRNA expression level in ontogeny of fish and different organs was evaluated by reverse transcriptase PCR (RT-PCR). The result showed the expression level of rag1, rag2 and IgM mRNA raised after 13, 13 and 22 dpf of fries respectively. This data was suggested that orange-spotted grouper at this stage might possess mature immunity and is able to produce immunoglobulin. The expression of rag1 was observed in thymus, head kidney and trunk kidney. The expression of rag2 was observed in thymus and head kidney. The expression of IgM was observed in thymus, head kidney, trunk kidney, spleen, intestines and pancreas. This data forms the basis for a proposal that the thymus and head kidney of teleost species play an essential developmental role in lymphopoiesis and thus can be regarded as a primary lymphoid organ.
Manyoni, Julian R. "Prophets of rage : expressions of Black nationalism in hip hop." Thesis, 2000. http://hdl.handle.net/2429/10663.
Повний текст джерелаFORMICHI, CATERINA. "CEBPb expression pattern regulates RAP1 protein levels in adipose tissue of obese patients and distinguishes subjects with metabolic impairment." Doctoral thesis, 2020. http://hdl.handle.net/11573/1362185.
Повний текст джерелаRösch, Daniela [Verfasser]. "Regulation der Expression der Rezeptoren für advanced glycation end products (RAGE) auf humanen Monozyten / vorgelegt von Daniela Rösch." 2005. http://d-nb.info/99540819X/34.
Повний текст джерелаWan-Hsuan, Tung, and 董宛璇. "The Role of Rac1/MLK3/JNK/AP-1 in CTGF-Induced Type I Collagen Expression in Lung Fibroblasts." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/53899417254003645945.
Повний текст джерела臺北醫學大學
醫學科學研究所
98
Several evidences suggest that connective tissue growth factor (CTGF) overproduction underlies the development of lung fibrosis. Development of lung fibrosis is characterized by excessive deposition of collagens in the lung interstitium. Mixed linage kinase 3 (MLK3) can be regulated by Rac and then activate downstream molecule JNK, which in turns activates transcription factor activator protein-1(AP-1). In this study, we found that CTGF could induce collagen expression, and overexpression of wild-type MLK3 also enhanced collagen expression. Overexpressed dominant negative of MLK3 (MLK3 DN) and K252a (a MLK3 inhibtor) concentration-dependently inhibited CTGF-induced collagen expression. CTGF also induced phosphorylation of MLK3 at Thr277/Ser281. Pretreatment of SP600125 (a JNK inhibtor) or transfection with JNK1/2 DN decreased CTGF-induced collagen expression in WI38 fibroblasts. CTGF also induced JNK phosphorylation in time-dependent manner. We also found that curcumin (an AP-1 inhibtor) reduced CTGF-induced collagen expression in a concentration-dependent manner. CTGF induced increase in c-Jun phosphorylation and AP-1-luciferase activity and also increased binding of AP-1 to collagen promoter region. In addition, CTGF induced increases in Rac1 activity, and RacN17 DN inhibit CTGF-induced collagen expression, MLK3 phosphorylation and JNK phosphorylation. CTGF-mediated AP-1 activation was inhibited by RacN17 DN、MLK3 DN and JNK1/2 DN. Taken together, these results suggest that the Rac1/MLK3-dependent JNK/AP-1 signaling pathway plays an important roles in CTGF-induced collagen expression in WI38 fibroblasts.
Wang, Chun-Chieh, and 王俊傑. "Cloning of rag-1 and Ig gene from cobia, Rachycentron canadum, and their expression during ontogenesis." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/k75y3u.
Повний текст джерела國立成功大學
生物科技研究所碩博士班
90
Immunoglobulin produced by B lymphocytes is a major protein involved in the humoral immune response. The variable region of Ig gene is assembled during B lymphocyte development by the recombination of multiple V, D, J segments. This V(D)J rearrangement process generates a vast array of antigen receptors and is strictly mediated by RAG recombinase, encoded by recombination activation gene-1 and -2. Because rag-1 gene, especially in the enzyme-activating core region, is highly conserved among species from teleost to mammals and is critical to the differentiation of pre-B cells, its expression within an associated primary lymphoid organ can serve as an ideal developmental marker to determine the location of immune organs. Besides, in order to study the maturity level of immune system with respect to time during cobia ontogeny, the expression patterns of rag-1 and Ig gene at different cobia growth stages (from embryo to fry stages) were examined. In addition to the use of β-actin gene as an internal control, partial rag-1 and Ig genes form cobia, Rachycentron canadum, each with an amplified size 637bp and 225bp respectively, were successfully clones. When compared with other previously reported rag-1 or Ig sequences of different fish species, the predicted rag-1 and Ig amino acid sequences of cobia displayed a minimum of 87% and 33% similarity. Tissue-specific expression of rag-1 was examined both by northern blotting and RT-PCR assay. The highest level of rag-1 expression was observed in the thymus, and a weaker expression was observed in spleen and kidney. This result indicated that thymus might be the most important immune organ compared to spleen and kidney.The expression patterns of rag-1 and Ig genes were both detected on the 7th day after hatching by RT-PCR assay. This suggested that cobia at this stage might possess mature immunity and is able to produce immunoglobulin.
Hagel, Marion [Verfasser]. "Hemmende Wirkung von Östrogenen auf die Rac1-Expression und die Freisetzung von Sauerstoffradikalen in humanen Monozyten / vorgelegt von Marion Hagel." 2008. http://d-nb.info/996205101/34.
Повний текст джерелаHausherr, Carolin Kim [Verfasser]. "Konditionale Expression von HER-2, H-Ras oder BXB-Raf1 in einem Maustumormodell : molekuare [molekulare] Mechanismen der Tumorremission / Carolin Kim Hausherr." 2005. http://d-nb.info/975920693/34.
Повний текст джерелаFaber, Claudius. "Die Expression der Rekombination Aktivierenden Gene (RAG) in Gedächtnis B Zellen von Kindern mit ANA-positiver Juveniler Idiopathischer Arthritis." Doctoral thesis, 2006. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-23099.
Повний текст джерелаWe examined memory-B-cells from children with ANA-positive Juvenile Idiopathic Arthritis for the expression of Recombination Activating Genes
Muth, Ingrid Elisabeth. "Die Expression von High Mobility Group Box 1 (HMGB1) und dessen Receptor for Advanced Glycation Endproducts (RAGE) als Pathomechanismus der sporadischen Einschlusskörpermyositis." Doctoral thesis, 2009. http://hdl.handle.net/11858/00-1735-0000-0006-AF6D-C.
Повний текст джерелаNitti, Maria. "Interfering with Rac1 activity in FRT thyroid epithelial cells impairs the expression of the polarized phenotype and of the E-cadherin function." Tesi di dottorato, 2013. http://www.fedoa.unina.it/9450/1/nitti_maria_25.pdf.
Повний текст джерелаFélix, Daniela Ribeiro Bettencourt. "As GTPases RAC1 e RAC1b na modulação da expressão do simportador de sódio e iodo em tecido tiroideu normal." Master's thesis, 2017. http://hdl.handle.net/10362/26982.
Повний текст джерелаThe sodium iodide symporter (NIS) is highly expressed in thyroid tissue. Since the expression of NIS results in the accumulation of iodide, its expression in tumor cells allows the use of radioactive iodine (131I) as a diagnostic and therapeutic tool. Recent studies showed that, although triggered by different intracellular signaling pathways, stimulation of NIS expression involves the induction of the p38 mitogenic kinase activity by the GTPase RAC1. Our group has recently described the overexpression of the RAC1b protein (a hyperactive variant of RAC1) in a number of papillary thyroid carcinomas with unfavorable outcome, carrying the activating mutation V600E in the mitogenic kinase BRAF. Notably, the presence of BRAF V600E mutation has been associated with downregulation of NIS. The aim of the present study was to investigate the role of RAC1/1b GTPases in modulating the expression of the symporter and also to develop a reporter system to study and identify post-translational regulators of this symporter. Using a normal thyroid cell line, the results obtained support a role for RAC1/1b signaling in the regulation of NIS expression. RAC1b was shown to play a role in inhibiting the expression levels of the symporter, whereas RAC1 seems to exert the opposite effect, since inhibition of endogenous RAC1 decreases the expression of NIS and it overexpression induces the activation of mitogenic p38 kinase. This study supports a role for RAC1/1b signaling in the regulation of NIS expression. The identification of new modulators of the functional levels of this symporter will be extremely relevant for the development of therapeutic strategies that allow an increase in the efficiency in 131I uptake by tumor cells.
Financiado por Bolsa Prof. E. Limbert Sociedade Portuguesa de Endocrinologia Diabetes e Metabolismo / Sanofi-Genzyme em Patologia da Tiróide
Faber, Claudius [Verfasser]. "Die Expression der Rekombination-aktivierenden Gene (RAG) in Gedächtnis-B-Zellen von Kindern mit ANA-positiver juveniler idiopathischer Arthritis / vorgelegt von Claudius Faber." 2007. http://d-nb.info/984710825/34.
Повний текст джерелаWeidmann, Rolf Günter [Verfasser]. "Endothel und Regulation der Inflammation : Überexpression inaktiver Mutanten der kleinen GTP-bindenden Proteine RhoA/Rac1/Cdc42 inhibiert die LPS-induzierte Expression von Interleukin-8/CXCL8 in humanen mikrovaskulären Endothelzellen / von Rolf Günter Weidmann." 2005. http://d-nb.info/978803736/34.
Повний текст джерелаKanellopoulou, Chrysi [Verfasser]. "Analysis of locus accessibility for V(D)J recombination and its potential in generating a mouse model for monitoring RAG protein expression in peripheral B cells / vorgelegt von Chrysi Kanellopoulou." 2001. http://d-nb.info/965433579/34.
Повний текст джерелаKarol, Sven. "Well-Formed and Scalable Invasive Software Composition." Doctoral thesis, 2014. https://tud.qucosa.de/id/qucosa%3A28724.
Повний текст джерела