Дисертації з теми "PWG-1"
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Greenhut, William Bradley. "Development of an asporogenic mutant of Bacillus licheniformis PWD-1." NCSU, 2003. http://www.lib.ncsu.edu/theses/available/etd-12172003-143418/.
Повний текст джерелаAl, Maskari Raya. "Large artery stiffness : genes and pathways." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277874.
Повний текст джерелаVrabec, Miroslav. "Řídící jednotka pro aeroponický pěstební systém." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2013. http://www.nusl.cz/ntk/nusl-219914.
Повний текст джерелаCarneiro, André Luis Machado. "Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte." Universidade Federal de Goiás, 2017. http://repositorio.bc.ufg.br/tede/handle/tede/7054.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The objective of this study was to evaluate the effect of adding the protease enzyme 0.05% (Cibenza DP 100) in diets for broilers based on corn and soybean meal with two nutritional matrices. Two experiments were conducted with 5 treatments with a basal corn and soybean meal diet: T1 – basal diet with valued protease matrix twice without the enzyme, T2 – basal diet with valued protease nutritional matrix once without enzyme, T3 – basal diet, T4 – basal diet with valued protease nutritional matrix twice with enzyme and T5 – basal diet with valued protease nutritional matrix once with enzyme. The first experiment was carried out with 1280 chicks Cobb500 strain of 1 to 42 days and the second experiment was carried out with 120 chicks housed in the first experiment, and the chicks were housed in battery cages from 17 to 21 days for digestibility study. The experimental design of the experiments was a randomized block, being in the first experiment the row in the experimental industrial shed the criteria for distribution of the blocks, with 5 treatments and 8 repetitions each containing 32 broilers per box. In the second experiment the floor of the cage was the criteria distribution of the blocks, with 5 treatments and 6 repetitions each containing 4 broilers per cage. The analyzed variables were: digestibility, carcass yield, rest, thighs, wings, abdominal fat and performance variables. In all treatments the data were subjected to analysis of variance, evaluated by the R program, and the means were compared by Tukey test at 10% significance. In the first experiment, it was observed a statistical difference (P <0.10) in the final mean weight, weight gain and feed intake in the pre-initial phase with beneficial results with the enzyme supplementation with valued protease nutritional matrix once. In the period from 1 to 21 days, there was a statistical difference (P <0.10) in feed intake and feed conversion, but with positive results for the control treatment. In the cut yield, there was no statistical difference (P> 0.10) in the most used cuts, similar results between the treatments and the control. Statistically different result (P <0.10) was found with the lowest proportion of abdominal fat in the diet fed with diet supplemented with the enzyme and nutritional matrix valued at one time. In the second experiment, statistical difference (P <0.10) was observed for nitrogen balance, which was positive, resulting in higher retention of nitrogen with lower excretion in the environment. The dry matter retention also showed a statistically different result (P <0.10), with a positive result for the supplementation with the enzyme together with greater metabolizability of the dry matter. Based on this study, the protease enzyme supplementation is recommended along with a valued protease nutritional matrix once, in the pre-initial phase of life of the birds, in order to have a better final weight, better weight gain, lower feed intake, lower proportion of abdominal fat, positive nitrogen balance and higher dry matter retention. Based on this work, it is recommended to supplement the protease enzyme with a valued protease nutritional matrix, especially in the pre-initial phase, in order to have a better final weight, better weight gain, lower feed intake, lower abdominal fat ratio, positive nitrogen balance and higher dry matter retention. In the other stages of life of the birds (initial, growth and final) the enzyme supplementation with a valued protease nutritional matrix was also beneficial, since the results in performance, cut yield and metabolizability were equivalent to control. The treatment diets are reduced in nutritional levels if compared to control diet with ideal dietary level for the broilers, resulting in less expense with the nutrients and maintaining a high yield.
Objetivou-se com a presente pesquisa avaliar o efeito da adição da enzima protease 0,05% (Cibenza DP 100) em dietas para frangos de corte a base de milho e farelo de soja. Foram conduzidos dois experimentos com 5 tratamentos cada. Todas as rações foram elaboradas com milho e farelo de soja, diferenciando no seguinte: T1 - ração basal com a matriz da protease valorizada em duas vezes sem a enzima, T2 - ração basal com a matriz da protease valorizada em uma vez sem a enzima, T3 - ração basal, T4 - ração basal com a matriz da protease valorizada em duas vezes com a enzima e T5 – ração basal com a matriz da protease valorizada em uma vez com a enzima. O primeiro experimento foi realizado com 1280 pintos da linhagem Cobb500 de 1 a 42 dias e o segundo com 120 pintos alojados em baterias de gaiolas de 17 a 21 dias para ensaio de digestibilidade. O delineamento experimental foi em blocos casualizados, sendo no experimento 1 a localização da parcela experimental dentro do galpão, com cinco tratamentos e oito repetições cada, contendo 32 aves por box. No experimento 2 o andar da gaiola foi o critério de distribuição dos blocos, com 5 tratamentos e 6 repetições cada, contendo 4 aves por gaiola. As variáveis analisadas nos experimentos 1 e 2 foram: digestibilidade, rendimento de carcaça, peito, coxas, asas, gordura abdominal e variáveis de desempenho. Os dados foram submetidos a analise de variância, avaliados por meio do programa R, e as médias comparadas pelo teste de Tukey a 10% de significância. No primeiro experimento, observou-se diferença estatística (P<0,10) no peso médio final, ganho de peso e consumo de ração na fase pré-inicial com resultados benéficos com a suplementação da enzima com valorização em uma vez da matriz nutricional. Já no período de 1 a 21 dias, houve diferença estatística (P<0,10) no consumo de ração e conversão alimentar, porém com resultados positivos para o tratamento controle. No rendimento de cortes, não houve nenhuma diferença estatística (P>0,10) nos cortes mais utilizados, resultados semelhantes entre os tratamentos e o controle. Resultado diferente estatisticamente (P<0,10) foi encontrado com a menor proporção de gordura abdominal na parcela alimentada com dieta suplementada com a enzima e matriz nutricional valorizada em uma vez. No segundo experimento, observou-se diferença estatística (P<0,10) para balanço de nitrogênio sendo este positivo, resultando em maior retenção de nitrogênio com menor excreção no ambiente. A retenção de matéria seca também mostrou um resultado diferente estatisticamente (P<0,10), com resultado positivo para a suplementação com a enzima juntamente com maior metabolizabilidade da matéria seca. Baseado nesse trabalho recomenda-se a suplementação da enzima protease juntamente com uma valorização da matriz nutricional, principalmente na fase pré-inicial, para que se tenha melhor peso médio final, melhor ganho de peso, menor consumo de ração, menor proporção de gordura abdominal, balanço positivo de nitrogênio e maior retenção de matéria seca. Nas outras fases de vida das aves (inicial, crescimento e final) a suplementação da enzima com a valorização da matriz nutricional também se mostrou benéfica, visto que os resultados em desempenho, rendimento de cortes e metabolizabilidade foram equivalentes ao do tratamento controle. As dietas tratamento são reduzidas em níveis nutricionais se comparadas com a dieta controle com o nível dietético ideal para as aves, isso acaba resultando em menor gasto com os nutrientes e mantendo uma alta produção.
MAI, LUIZ A. "Analise tecnico-economico do ciclo de combustivel 'Tandem'. Um estudo do caso Brasil-Argentina." reponame:Repositório Institucional do IPEN, 1997. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10684.
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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
Bjarnegård, Niclas. "Aspects on wall properties of the brachial artery in man : with special reference to SLE and insulin-dependent diabetes mellitus." Doctoral thesis, Linköpings universitet, Institutionen för medicin och hälsa, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-11273.
Повний текст джерелаFER, NELSON C. "Medidas de parametros neutronicos de veneno queimavel de Alsub(2)Osub(3)-Bsub(4)C para reatores PWR." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10851.
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Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
Ayhan, Ufuk. "Investigation Of Dc Bus Current Harmonics In Two And Three Level Three-phase Inverters." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614182/index.pdf.
Повний текст джерелаKaraduman, Ferdi. "Adaptive Control Of Dc Link Current In Current Source Converter Based Statcom For Improving Its Power Losses." Master's thesis, METU, 2013. http://etd.lib.metu.edu.tr/upload/12615310/index.pdf.
Повний текст джерелаBini, S. "EFFETTO DELLA VELOCITÀ DI ASSUNZIONE DI CIBO E DELLA PALATABILITA¿ SULLA SECREZIONE DI PEPTIDI GASTROINTESTINALI NELL¿ OBESITA¿ SEMPLICE E GENETICA." Doctoral thesis, Università degli Studi di Milano, 2016. http://hdl.handle.net/2434/354179.
Повний текст джерелаTříska, Vít. "Univerzální řídicí jednotka solárních kolektorů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2010. http://www.nusl.cz/ntk/nusl-237100.
Повний текст джерелаUslu, Mutlu. "Analysis, Design, And Implementation Of A 5 Kw Zero Voltage Switching Phase-shifted Full-bridge Dc/dc Converter Based Power Supply For Arc Welding Machines." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/3/12607873/index.pdf.
Повний текст джерелаCASTANHEIRA, MYRTHES. "Analise dos mecanismos de degradacao de varetas combustiveis falhadas em reatores PWR." reponame:Repositório Institucional do IPEN, 2004. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11141.
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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
Yildirim, Dogan. "Field Oriented Control Of A Permanent Magnet Synchronous Motor Using Space Vector Modulated Direct Ac-ac Matrix Converter." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614302/index.pdf.
Повний текст джерелаAC matrix converter based surface mounted permanent magnet synchronous motor (PMSM) drive system. First, the matrix converter topologies are analyzed and the state-space equations describing the system have been derived in terms of the input and output variables. After that, matrix converter commutation and modulation methods are investigated. A four-step commutation technique based on output current direction provides safe commutation between the matrix converter switches. Then, the matrix converter is simulated for both the open-loop and the closed-loop control. For the closed-loop control, a current regulator (PI controller) controls the output currents and their phase angles. Advanced pulse width modulation and control techniques, such as space vector pulse width modulation and field oriented control, have been used for the closed-loop control of the system. Next, a model of diode-rectified two-level voltage source inverter is developed for simulations. A comparative study of indirect space vector modulated direct matrix converter and space vector modulated diode-rectified two-level voltage source inverter is given in terms of input/output waveforms to verify that the matrix converter fulfills the two-level voltage source inverter operation. Following the verification of matrix converter operation comparing with the diode-rectified two-level voltage source inverter, the simulation model of permanent magnet motor drive system is implemented. Also, a direct matrix converter prototype is constructed for experimental verifications of the results. As a first step in experimental works, filter types are investigated and a three-phase input filter is constructed to reduce the harmonic pollution. Then, direct matrix converter power circuitry and gate-driver circuitry are designed and constructed. To control the matrix switches, the control algorithm is implemented using a DSP and a FPGA. This digital control system measures the output currents and the input voltages with the aid of sensors and controls the matrix converter switches to produce the required PWM pattern to synthesize the reference input current and output voltage vectors, as well. Finally, the simulation results are tested and supported by laboratory experiments involving both an R-L load and a permanent magnet synchronous motor load. During the tests, the line-to-line supply voltage is set to 26 V peak value and a 400 V/3.5 kW surface mounted permanent magnet motor is used.
Kumar, Kranthi J. R. A. "Studies on Single and Two Stage Stirling Type Pulse Tube Coolers of Low and Medium Capacities including Performance Enhancement of Pressure Wave GeneratoI and a Novel Helium Recondensation System." Thesis, 2017. http://etd.iisc.ac.in/handle/2005/4224.
Повний текст джерела鄧燕妮. "Molecular cloning of keratinase gene from bacillus licheniformis PWD-1." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/12039280097513426183.
Повний текст джерела鄭淑文. "Studies on the keratinase of bacillus licheniformis PWD-1 : production, purification, characterization and gene cloning." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/14298703500468442520.
Повний текст джерела林青平. "Studying the Structure and Function of Keratinase from Bacillus licheniformis PWD-1 by Site-directed Mutagenesis." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/26977971441904499507.
Повний текст джерела國立海洋大學
水產生物技術研究所
86
The Bacillus licheniformis PWD-1 keratinase gene was subcloned in pET-20b(+), an transfer it into several E. coil strains. The transformants were fatal. A recombinant keratinase was constructed with a thioredoxin extended on its N-terminus by genetic engineering. However, the ftision protein lost keratinase activity. This suggests that the keratinase activity could be a toxic to the E. coli. The keratinase shares a high similarity (98.95%) with the subtilisin Carlsberg, whose three-dimantional structure has been resolved. According to the computer simulation, keratinase was .rationally modified by site-directed mutagenesis. A imitant with double mutation sites, Gly 61/Cys 61 and Ser 98/Cys 98, was constructed to improve keratinase thermostability by introducing a disulfide bond. Both the wild type and mutant enzymes exhibited the same optimal temperature, 50. However, the mutant enzymes displayed higher enzyme activity at temperature (40-90 ℃) and pH (6-10) than the wild .type. In thermostability study, a 2-3 ℃ improvement was observed in the mutant, and the improvement is lost after DTT treatment. It suggested that Cys 61 and Cys 98 successfilUy formed a disulfide bond. In thermoinactivation kinetic study, the Ea of the wild type and mutated keratinase were 57 and 100 kcal/mol, respectively. The half-life of the mutant was over two times longer than wild type keratinase. It concludes, we have demonstrated the feasibility of protein engineering to increase the heat stability and improve the biochemical properties of keratinase for industrial applications.