Дисертації з теми "Pure culture"
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1
Porcu, Elisabetta. "Pure Land Buddhism in modern Japanese culture /." Leiden : Brill, 2008. http://catalogue.bnf.fr/ark:/12148/cb413311546.
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2
MAZURE, NATHALIE. "Etude de la biodegradation de la morphine par une culture pure mycobacterium aurum, et les cultures mixtes, pseudomonas." Compiègne, 1993. http://www.theses.fr/1993COMP648S.
Повний текст джерелаАнотація:
La morpholine est un compose heterocyclique sature. C'est une base forte et ses proprietes de solvant en font un compose couramment utilise dans l'industrie chimique. Compose xenobiotique, la morpholine a ete classee pendant longtemps comme tres persistante. Cependant, l'isolement recent de cultures capables de la degrader l'a sortie definitivement des listes des composes non biodegradables. Les connaissances sur la biodegradabilite de la morpholine se resume en une voie de degradation hypothetique etablie a partir de mycobacterium chelonae. Dans ce travail, nous avons choisi d'aborder l'etude de la degradation de la morpholine en menant de front la microbiologie et la biologie moleculaire de deux populations, l'une pure (mycobacterium aurum: gram-positif) et l'autre mixte, essentiellement composee de pseudomonas (gram-negatif). Avec m. Aurum, nous avons etabli ses capacites degradatives et l'interet de l'immobilisation et observe l'existence d'une gangue en presence de morpholine, ainsi que d'un mecanisme de regulation des deux branches de la voie de degradation. Les genes de la morpholine ne s'exprimant pas ou peu dans e. Coli le392, des mutants mor- et eta- ont ete obtenus. La culture mixte nous a permis de suivre l'evolution d'une culture apres deux annees de degradation dans une station d'epuration et d'observer l'augmentation de ses capacites degradatives. Aucun phenotype relatif a la degradation n'a pu etre impute a la presence de grands plasmides dans l'une et l'autre des cultures malgre les echanges genetiques observes. Ces deux cultures ont montre que l'ouverture du cycle se faisait au niveau de liaison c-n et que la presence d'une double liaison l'empechait. Il semble que dans les cas, les genes soient chromosomiques
3
Zarnegar, Abdolreza. "Purification and Characterization of an Inhibitor of Thymidine Uptake From Culture Supernatants of Human Tonsil Lymphocytes." Digital Commons @ East Tennessee State University, 1987. https://dc.etsu.edu/etd/2833.
Повний текст джерелаАнотація:
Lymphocytes from human tonsils were cultured in the absence of serum for 3 days. In the presence of the concentrated culture supernatant the proliferative response of PBL, to con A, as measured by the uptake of ('3)H-tdr, was significantly reduced. The suppressor substance was referred to as SMAL (suppressor of mitogen activated lymphocytes). The estimated molecular weight of SMAL under nondenaturing conditions was 100,000-300,000. SMAL also suppressed the incorporation of ('3)H-tdr by a variety of mouse and human tumor cell lines. The activity of SMAL was sensitive to pronase and heating at 100(DEGREES)C for 30 minutes but insensitive to RNase. Treatment with DNase, however, enhanced the activity of SMAL. SMAL activity was also destroyed by treatment with 5% TCA, 0.4 M HCl or 60% acetonitrile, but resistant to 6 M urea or dialysis against pH 2 buffer for 24 hours. SMAL activity was precipitated in 40-80% ammonium sulfate saturation. When applied to a phenyl-sepharose column no activity was recovered. SMAL was not produced by heat-killed tonsil lymphocytes or lymphocytes-treated with cycloheximide. Maximal production occurred in the first 24 hours of culture, and progressively less was produced in subsequent 24-hour intervals. Both T- and B lymphocyte-enriched culture supernatants contained SMAL. SMAL adhered strongly to DEAE-cellulose, but less than two-fold purification was achieved. Using QMA-Accell anion exchange medium, a 5-fold purification of SMAL with higher specific activity was obtained with HPLC. Activity of SMAL was recovered after native polyacrylamide gel electrophoresis by electroblotting to DEAE-cellulose paper followed by eluting the bound materials with salt. Two active components, one corresponding to a large and/or less negatively charged molecule and another corresponding to a small and/or highly acidic molecule, were recovered. HPLC-purified SMAL at relatively low doses inhibited the uptake and phosphorylation of ('3)H-tdr, without significant effect on cell proliferation. The inhibition of ('3)H-tdr uptake was favored over that of ('3)H-udr or ('3)H-adr, and this effect was reversible. At relatively high doses of HPLC-purified SMAL, the growth of mouse thymoma EL-4 and human T cell leukemia CEM-CM(,3) cell lines was inhibited.
4
Brading, Melanie Gayle. "The influence of fluid dynamics and surface material on pure and binary culture biofilms." Thesis, University of Exeter, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307314.
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5
Wang, Hao. "Development and electrochemical characterization of a Pseudomonas aeruginosa-based pure culture microbial fuel cell." University of Dayton / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1311958719.
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6
Lamballe, Fabienne. "Reparation du dna dans les hepatocytes de rat adulte en culture pure et co-culture : mecanismes enzymatiques et expression d'oncogenes nucleaires." Rennes 1, 1989. http://www.theses.fr/1989REN10038.
Повний текст джерелаАнотація:
Deux modeles experimentaux disponibles: la culture pure d'hepatocytes de rat et la co-culture lorsque ces derniers sont associes avec les cellules epitheliales hepatiques. Dans les deux systemes etudies, deux dna ligases distinctes de part leurs proprietes catalytiques ont ete identifiees. Apres irradiation uv, seule la ligase ii est stimulee. Cette enzyme semble par consequent etre impliquee dans la reparation du dna. Cette stimulation correspond a une synthese de novo, mais il semble n'y avoir activation de la transcription que dans le modele de co-culture. Apres irradiation , les dna polymerases et ont stimulees en culture pure alors qu'en co-culture, seule la polymerase est activee. Apres irradiation uv, seule la polymerase semble etre activee. L'etude de l'expression d'oncogenes nucleaires montre que c-myc est stimule apres irradiation des cellules alors que l'expression de c-myb ne semble pas etre modifiee
7
Bally, Matthias. "Physiology and ecology of nitrilotriacetate degrading bacteria in pure culture, activated sludge and surface waters /." [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10821.
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8
Qiu, Yu-Shan. "Development of a successful method for quantifying viable oral anaerobic spirochetes from pure culture and periodontal pockets." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68246.
Повний текст джерелаАнотація:
Spirochetes are markedly prevalent in periodontal disease but are not included as predominant cultivable organisms because of the inability to quantify them by viable count. A successful method was developed for enumerating viable oral spirochetes in pure culture as colony-forming units (CFU) in new oral spirochete (NOS) medium with 0.7% agarose and using small tissue-culture flasks. Three species of oral spirochetes in log-phase growth in NOS broth were used for evaluation of the method. Reliable, consistent and reproducible viable counts of pure spirochete cultures were obtained.This method was extended to enumerate viable oral spirochetes from periodontal pockets. The antibiotic rifampin (20 $ mu$g/ml) was found to be an excellent selective agent for such a count when added to NOS-agarose medium. Counts of cultivable oral spirochetes from 10 subgingival plaque samples ranged from 12.5% to 28.2% of the total cultivable anaerobic bacteria. In addition, by the use of this method thirteen new oral spirochetes were isolated.
The viable count technique was modified and employed to study the locomotion of spirochetes. Migration of oral spirochetes out of NOS-Bacto agar medium into NOS-agarose medium was observed and two locomotory phenotypes of oral spirochetes were detected.
9
Kijkla, Pruch. "Biocide Mitigation of Carbon Steel and Stainless Steel Biocorrosion by Pure-Strain and Mixed-Culture Microbial Biofilms." Ohio University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1619007982435067.
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10
Bertrand, François. "Les oidiums des cucurbitacees : maintien en culture pure, etude de leur variabilite et de la sensibilite chez le melon." Paris 11, 1991. http://www.theses.fr/1991PA112286.
Повний текст джерелаАнотація:
Des isolats monoconidiens des oidiums des cucurbitacees (sphaerotheca fuliginea et erysiphe cichoracearum, en abrege s. F. Et e. C. ) sont maintenus en culture pure sur de jeunes cotyledons de concombre excises places sur un milieu saccharose-mannitol gelose. In vitro, la sporulation est reduite a l'obscurite ou par inhibition chimique de la photosynthese, accrue par apport de glucides ou de glycerol, legerement accrue par le mannitol, inhibee par l'inositol. S. F. Et e. C. Different par leur comportement en cas d'absence de photoperiode, d'augmentation d'eclairement, de baisse d'humidite relative et par leur germination sur de l'eau gelosee. Sur 147 echantillons de plantes parasitees collectes de 1987 a 1989, s. F. Domine en ete, e. C. Est plus frequent au printemps et sous abris. Des pathotypes se distinguent sur concombre (a) cv. Marketer, melon (b) cv. Vedrantais (1) et p. M. R. 45 (2), courgette (c) cv. Diamant et pasteque (d) cv. Sugar baby. Les pathotypes de e. C. (a, ab(1,2), ac et acd) sont ubiquistes. Pour s. F. , le pathotype a est seul trouve dans le nord de la france, ab(1,2)c domine dans le sud. Les clones de s. F. Se classent en 2 groupes de compatibilite sexuelle complementaires; 86 clones produisent peu de cleistotheces, 5 sont tres productifs. Chez e. C. , les clones a sont sexuellement compatibles avec les clones ac et acd, les clones ab ne forment pas de cleistotheces. Sur 456 genotypes de melon, 4 sont sensibles a s. F. A et e. C. A, 151 sont resistants a e. C. Ab et sensibles a s. F. Abc, 35 sont resistants a s. F. Et sensibles a e. C. Ab. La resistance a s. F. Ab(1,2)c n'est jamais trouvee sans la resistance a s. F. Ab(1)c
11
Patureau, Dominique. "Etudes cinétique et physiologique d'une bactérie dénitrifiant en conditions aérobies. Suivi en réacteur aéré, parfaitement mélangé, en culture pure et en culture mixte associée à une flore nitrifiante." Toulouse, INSA, 1995. http://www.theses.fr/1995ISAT0034.
Повний текст джерелаАнотація:
Ce travail repose sur l'etude d'une souche, nommee sgly2, isolee d'un reacteur anoxique, qui presente un comportement particulier vis-a-vis de l'oxygene. Elle est, en effet, capable de reduire simultanement nitrate (ou nitrite ou protoxyde d'azote) et oxygene, et ce, jusqu'a des concentrations en oxygene dissous non limitantes. Le principal produit de la reduction du nitrate est alors l'azote moleculaire. Les cultures en mode continu n'ont fait que confirmer ces premieres observations. Les effets compares d'inhibiteurs de la chaine respiratoire et de la synthese proteique, de detergent, sur la souche sgly2 soumise a differentes conditions d'aeration, ont permis de mettre en evidence une partie des mecanismes grace auxquels cette souche est capable de fonctionner en presence d'oxygene: (i) expression d'une nitrate reductase quelles que soient les conditions de culture, (ii) existence d'un double systeme enzymatique. Ces proprietes particulieres de co-respiration de la souche sgly2 ont ensuite ete utilisees dans la realisation d'une co-culture associant, dans un meme reacteur aerobie, une flore nitrifiante complexe et le denitrifiant aerobie. Le maintien de la souche sgly2 s'est fait par ajout de facon intermittente de la source de carbone. Dans ces conditions, une activite simultanee de la flore nitrifiante et du denitrifiant a ete observee, avec reduction aerobie des n-oxydes produits par les autotrophes en protoxyde d'azote et azote
12
Griffiths, Jonathan T. H. "The effect of temperature upon the growth and metabolism of Aeromonas hydrophila and Lactobacillus plantarum in pure and mixed culture." Thesis, Oxford Brookes University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363793.
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13
Lam, Melissa Karmen. "'The Pure Products of America Go Crazy' Defamiliarizing American Language and Culture in Lolita and The Crying of Lot 49." Thesis, University of Canterbury. English, 2006. http://hdl.handle.net/10092/942.
Повний текст джерелаАнотація:
My thesis centers on Lolita and The Crying of Lot 49 and the ways in which both novels defamiliarize our world and ways of thinking. Both novels use formal literary techniques as a way of making ordinary cultural artifacts, situations, and environments seem unfamiliar from our every day perceptions. This process of defamiliarizing the regular and everyday has the greater implications of estranging universal themes such as love, environment, and belonging. Both novels also question our precarious hold on corporeal reality by interpreting plot through two outside narrators whose trustworthiness is constantly placed into question. Unsurprisingly, Lolita and The Crying of Lot 49 unsettle the categories of truthfulness and reinvention in interpreting America's immediate cultural and environmental landscape. Both texts blur the distinction between recorded and imaginatively reconstructed worlds: just so, America has isolated our two narrators in the text from their immediate landscape. Interpretations of America are questioned in the thesis through the process of Shklovsky's theory of Defamiliarization interfaced with Freud's Uncanny in the novel. Language disobedience and discord also play a part and will be discussed through Bakhtin's theories on polyphonic language.
14
Pakdee-Patrakorn, Chusak. "Recherches sur les potentialités fourragères du soja (Glycine max. (L. ) Merrill) : en culture pure et en association avec le mai͏̈s." Toulouse, INPT, 1986. http://www.theses.fr/1986INPT012A.
Повний текст джерелаАнотація:
Evaluation des potentialites fourrageres du soja par l'etude de l'evolution des compositions morphologique et chimique des quelques varietes appartenant a differents groupes de precocite. Determination de leur valeur alimentaire en vert, au cours d'une periode situee entre la floraison et la maturite physiologique. Ces varietes de soja mises en culture avec divers mais, ont permis d'apprecier leur comportement en culture associee, tant sur le plan du rendement que de leur valeur alimentaire. Enfin, des essais en cages a bilan realises sur un ensilage de mais-soja ont permis de confirmer l'interet technique de cette culture
15
Pakdee-Patrakorn, Chusak. "Recherches sur les potentialités fourragères du soja, Glycine max. (L.) Merrill, en culture pure et en association avec le maïs." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb37600205n.
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16
Persson, Johan, and Erik Eliason. "Ett schackbräde är inget utan sina pjäser : En beskrivande studie av användares deltagande hos nischade online pure players." Thesis, Linnéuniversitetet, Institutionen för medier och journalistik (MJ), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-45418.
Повний текст джерелаАнотація:
The purpose of this study was to describe participation in a new context in which niche journalism is combined with online communities. The majority of past research regarding participation in journalism is focused on traditional journalism and the definition of the term needs to be updated. The aim of the study was to describe participation amongst niche pure players. Using both quantitative and qualitative research methods, a survey study and semi-structured interviews were conducted. The two empirical aspects resulted in a perspective of participation amongst five pure players, sharing a similar niche. The results of the study showed that niche pure players create user participation by combining journalistic participation with participation in online communities. Empirical data showed a correlation between user participation in the two different sections of the sites. Users with higher participation in one of the sections also partook more in the other. Furthermore, another correlation found was that users who considered it important to be able to partake in a discussion of the journalistic content, also considered the online community to be of great importance to the site. Additionally, the study showed that there is an exchange of information and knowledge, being regarded by the users as something of high value. By providing a platform for horizontal communication between users in the form of a forum, in which users are able to freely create and share content, a participatory culture is created. This participatory culture further increases the degree of user participation in the journalistic content.
17
FRESSINAUD, MASDEFEIX CATHERINE. "Contribution a l'etude de la myelinisation in vitro : analyse des acides gras et etude ultrastructurale d'oligodendrocytes de rat myelinisant en culture pure." Limoges, 1988. http://www.theses.fr/1988LIMO0210.
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18
Neilson, James. "Attachment of bacteria to glass surfaces in pure culture and in mixed suspensions and the effect of growth conditions on that attachment." Thesis, University of Warwick, 1991. http://wrap.warwick.ac.uk/108883/.
Повний текст джерелаАнотація:
The attachment of selected freshwater bacteria, Aeromonas Chromobacterium, a coryneform and Staphylococcus In pure culture and in mixed suspensions with one other bacterium to glass surfaces was investigated in the laboratory. Changes in the nutrient conditions of the growth medium during growth and attachment and the temperature, pH and electrolyte type and concentration present in the attachment solution during attachment experiments all influenced bacterial attachment. The pure culture attachment results obtained depended on the bacterial species being investigated. When bacterial species were attached in the presence of one other species the growth conditions still had a profound effect on attachment. The results obtained depended on the bacterial species present in the mixed suspension, with some bacterial species having a more profound effect on the attachment of other species than others. The bacterial species used to study bacterial attachment in the laboratory were used along with Acinetobacter and Pseudomonas to investigate bacterial attachment in vivo using a model system. The attachment of these bacteria were investigated in two- to six-membered suspensions. The biofilms obtained in the model system consisted of smaller bacterial cells in a more densely packed biofilm. These biofilms could still be influenced by growth conditions as temperature was seen to influence the bacterial biofilm obtained. During these attachment experiments the Acinetobacter which did not attach itself had a profound effect on the attachment of other bacterial species when present in the liquid phase. The mechanism by which Acinetobacter inhibited the attachment of other bacteria was investigated and it appeared Acinetobacter did not excreate a chemical to influence the attachment of other species, but the Acinetobacter cells themselves had to be present in the liquid phase to influence the attachment of other species. The effects of different molecular weight fractions of Tocil Lake water were investigated for their effect on bacterial attachment. The fraction containing the > 30,000 MV component was found to influence bacterial attachment. The results obtained, depended on the bacterial species being investigated.
19
Chahatha, Hisham El. "Étude de quelques aspects écologiques des relations nutritionnelles entre graminées et légumineuses en présence d'un champignon endomycorhizien en culture pure et mixte (compétition, symbiose, pollution)." Nancy 1, 1991. http://www.theses.fr/1991NAN10181.
Повний текст джерелаАнотація:
Ière partie compétition entre graminée et légumineuse. Deux familles: la graminée et la légumineuse ayant un potentiel agronomique très important ont été étudiées du point de vue de leur compétition nutritionnelle. 2 plantes appartenant à ces deux familles ont été étudiées expérimentalement dans la culture pure et mixte dans des conditions de déficience minérale. La croissance de la plante, le contenu en ions dans la partie aérienne ainsi que dans la racine et la sève xylémique ont été étudiées. Les résultats montrent que les graminées sont mieux adaptées au potassium ou d'autres éléments minéraux déficients que la légumineuse quelque soit l'espèce. 2éme partie symbiose avec vam champignon. Dans cette partie, nous étudions l'effet de vam champignon glomus mosseae sur trois espèces de legumineuses (glycine max l. , medicago sativa l. Et trifolium repense l. ) Et une espèce de graminée (zea mays), la croissance et la composition minérale des plantes. Les plantes ont été cultivées dans un sol stérilisé ou en aquiculture et elles sont inoculées avec une racine infectée par vam champignon. Après 7 semaines de croissance des plantes, nous quantifions le développement des plantes, le pourcentage de mycorhization et la composition minérale des plantes. Nous avons constaté que les plantes inoculées avec vam champignon ont eu une meilleure accumulation des ions en comparaisons avec des témoins
20
Surtiningsih, Suprapto Tini. "Mobilisation de P et CD de phosphates naturels par des bactéries et des champignons ectomycorhiziens en culture pure et dans la rhizosphère du pin sylvestre." Nancy 1, 1994. http://www.theses.fr/1994NAN10271.
Повний текст джерелаАнотація:
L'influence d'une rhizobactérie solubilisant les phosphates (agrobacterium sp. ) et de champignons ectomycorhizogénes (pisolithus tinctorius, suillus granulatus, et hebeloma cylyndrosporum) sur la biodisponibilité du phosphore et du cadmium de trois phosphates naturels (Caroline du nord, Sénégal et Togo), a été étudiée: en cultures pures en l'absence de racines et dans la rhizosphère du pin sylvestre. En culture pure, agrobacterium sp. Mobilise plus de P. Et CD des phosphates naturels que les champignons ectomycorhizogénes étudiés. Cette mobilisation est surtout une solubilisation pour la bactérie, mais pour les champignons l'immobilisation est plus importante et atteint 80 à 100% du CD mobilisé. Dans la rhizosphère du pin sylvestre, la mycorhization par p. Tinctorius augmente le % de p mobilisé, et diminue la teneur en cd. Agrobacterium sp. Favorise la mycorhization par p. Tinctorius, la croissance des pins, la mobilisation et l'absorption de P et CD par ceux-ci. Les quantité de CD mobilisées par la bactérie et les champignons en culture pures sont plus importantes avec le phosphate de Caroline qu'avec les phosphates d'Afrique de l'ouest. Cependant dans les expériences avec plantes, le pourcentage de CD mobilisé ne semble pas toujours corrélé avec la teneur en CD des phosphates ni leur dureté
21
Bouzar, Fatouma. "Variabilité clonale de Lactobacillus delbrueckii subsp. Bulgaricus CNRZ 1187 : influence sur la production des polysaccharides exocellulaires au cours de la fermentation en culture pure et mixte." ENSIA, 1997. http://www.theses.fr/1997EIAA0065.
Повний текст джерелаАнотація:
La presente etude porte sur l'influence de la variabilite clonale de la souche epaississante lactobacillus delbrueckii subsp. Bulgaricus cnrz 1187 sur la production d'exopolysaccharides au cours de la fermentation du lait (24 heures) en culture pure et mixte avec une souche non epaississante de streptococcus thermophilus, et sur l'implication de ces polymeres dans la texture du yaourt. Deux clones producteurs d'exopolysaccharides, w (blanc) et p (rose) phenotypiquement stables, ont ete selectionnes au rouge de ruthenium. La variabilite clonale n'a que peu d'effet sur le comportement general de fermentation tel que la croissance, l'acidification et la consommation du lactose. Elle affecte, par contre, la production d'exopolysaccharides et leur implication dans le developpement de la viscosite des laits fermentes. La plus forte viscosite est developpee par la souche mere qui atteint un maximum de 280 mpa. S apres 8 heures de fermentation. Le variant w developpe la plus faible viscosite, et le variant p donne un resultat intermediaire. Le variant w produit la plus faible quantite d'exopolysaccharides, le variant p la plus forte et la souche mere produit une quantite intermediaire. En culture pure, la production d'exopolysaccharides debute en phase exponentielle et se poursuit en phase stationnaire. En culture mixte, cette production debute plus tot et elle est plus rapide. Les viscosites des laits fermentes par les cultures mixtes, ainsi que les quantites d'exopolysaccharides produites, sont plus elevees que celles obtenues en culture pure ; mais la relation entre les viscosites et les quantites d'exopolysaccharides produites differe. Selon qu'il s'agit de culture pure ou mixte. La souche mere en culture mixte produit le moins d'exopolysaccharides mais montre la plus forte viscosite. Le variant w, en culture mixte, produit la plus forte quantite d'exopolysaccharides mais developpe la plus faible viscosite. Le variant p montre une viscosite et une quantite d'exopolysaccharides intermediaires. Compares aux exopolysaccharides produits en culture pure, majoritairement composes de galactose et de glucose, les exopolysaccharides, formes en culture mixte contiennent en plus du rhamnose. Ceci suggere que les enzymes conduisant a la synthese des sucres nucleotides impliques dans la synthese des exopolysaccharides ne sont pas les memes du simple fait de la presence de s. Thermophilus. Les proportions en monosaccharides varient au cours de la fermentation differemment selon les souches. De plus, ces proportions varient seulement pendant les 6 premieres heures de fermentation en culture mixte, alors qu'elles ne se stabilisent qu'apres 10 heures en culture pure.
22
Vu, Thi Thu Tra [Verfasser]. "Investigation of Vibrio and ESBL/AmpC-producing Enterobacteriaceae in retail seafood and inactivation of Vibrio in pure culture and mussel homogenates using high hydrostatic pressure / Thi Thu Tra Vu." Berlin : Freie Universität Berlin, 2019. http://d-nb.info/117715272X/34.
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23
Skandrani, Dalila. "Détermination des seuils de toxicité de divers insecticides (forme pure ou commerciale) sur cellules humaines en culture (A549, SH-SY5Y) : expression des gènes et protéines de stress (HSPs, GRPs,…)." Toulouse 3, 2006. http://www.theses.fr/2006TOU30233.
Повний текст джерелаАнотація:
Ce travail a eu pour but de déterminer les concentrations cytotoxiques de divers insecticides (molécule pure versus formulation commerciale) qui induisaient une inhibition de la prolifération de cellules d'origine pulmonaire (A549) ou neuronale (SH-SY5Y) et d'analyser les variations d'expression de gènes de stress par la technique des cDNA arrays et/ou des protéines (HSP, GRP) sur western blot. Il a été montré que les formulations pouvaient être jusqu'à 150 fois plus toxiques que les molécules pures. Dans les cellules A549, les insecticides ont induit une surexpression de la GRP78 et une sous-expression des HSPs. Le formetanate (pur ou commercial) a induit une surexpression des transcrits GADD153 et diverses GST et UDPGT. Le Dicarzol a induit une surexpression des transcrits SOD2 et TOP2AToxicity of several insecticides was determined in vitro on lung adenocarcinoma A549 and neuroblastoma SH-SY5Y cell lines, with the aim to find out, among stress proteins, reliable and sensitive markers of occupational or accidental exposure. Carbamates (formétanate, methomyl, pyrimicarb), organochlorines (dienochlor, endosulfan), pyrethroid (bifenthrin) and neonicotinoid (imidacloprid) insecticides were comparatively investigated either as pure chemical or as commercial formulations. Measurement of threshold concentrations (LOEC) leading to a significant decrease of the growth-rate in A549 cells showed that organochlorines were the most toxic whereas imidacloprid and methomyl were the less toxic. SH-SY5Y cells were found to be more sensitive than A549. When compared at similar concentration of active principle, commercial formulations were found to be twice to 100 times more aggressive than the respective pure active molecule. In A549, GRP78 stress protein was up-regulated by almost all the insecticides, commercial formulations being more efficient. No such effect was observed in SH-SY5Y. Conversely, cytosolic HSP72/73 stress proteins were somewhat underexpressed in all cases. .
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Grateau, Philippe. "Sensibilités, cultures et doléances : regard culturel sur les cahiers de doléances de 1789." Rennes 2, 1999. http://books.openedition.org/pur/23366.
Повний текст джерелаАнотація:
Lus, exploites, publies, les cahiers de doléances ont déjà fait couler beaucoup d'encre, et des meilleures plumes, celles de Michelet, Taine, Tocqueville, Jaures, ou Furet. Soumis a toutes les grilles de lecture, de la lecture cursive a la lecture structurale, interroges sur ce qu'ils disent de la politique, de l'économie ou de la culture de leur temps, les cahiers de doléances ne semblent plus avoir de secrets. Pourtant, la commémoration scientifique du bicentenaire a été l'occasion d'en rappeler l'extraordinaire richesse. En proposer une << lecture culturelle >> n'est pas faire une découverte archivistique, mais c'est d'une part prendre en compte l'énorme production historique des trois dernières décennies et, d'autre part, bénéficier de l'immense effort de publication mis en oeuvre depuis plus d'un siècle. Les travaux récents dans le domaine de l'opinion publique ou de la culture matérielle permettent en effet un renouvellement des questionnements tandis que les nombreuses éditions nous autorisent une approche qualitative et quantitative en jouant sur les variations d'échelle. Apres une synthèse critique des travaux antérieurs, l'enquête, a la fois nationale et régionale, présente d'abord des éclairages sur les aspirations morales et philosophiques des ruraux, que ce soit sur le désir de liberté, d'égalité, de bonheur ou de progrès. Elle passe ensuite des représentations aux pratiques culturelles. En effet, au détour d'une plainte a caractère fiscal, la communauté paysanne décrit sa conception de l'autorité et de la souveraineté, elle dit sa crainte de manquer de pain, sapeur de la maladie ou encore sa soif de culture. Elle s'interroge sur les moyens de financer l'école, de se procurer des médicaments ou d'attirer au village le chirurgien et la sage-femme compétents. Ainsi, les petites gens nous montrent, le temps d'une réunion au moins, le devenir des rideaux du siècle des Lumières dans le cadre paroissialThe registers of grievances have been worked on, exploited and published. They have been widely commented upon especially by experts including Michelet,Taine, Tocqueville, Jaures or Furet. Since they have been subjected to all sorts of reading grids, ranging from cursive to structural reading, and searched for any relevant information they could disclose about the political, the economic and the cultural situation of the time, they seem to hold no more secrets. Yet, commemorating the bicentennial on a scientific level was the opportunity to remind every one of us of their extraordinary richness. Reading them from a cultural point of view isn't aimed at being considered as + scoop ;. It rather aims, on the one hand, at taking into account the immense historical production of the last three decades and on the other hand, at making the most of the product of the extraordinary effort which has been put into publication for over a century. Indeed, recent works in the field of public opinion or material culture raise new questions. Along the same hoes, the numerous available editions enable us to approach things from qualitative as well as a quantitative angle by examining them on different scales (local, regional, national). After presenting a critical synthesis of the existing works on the subject, the survey concerning both national and regional levels, casts a light on rural people's moral and philosophical aspirations, whether these convey their yearning for freedom, equality, happiness or progress. From these representations, it then goes on to explore cultural attitudes. For instance, through a complaint about the tax system, the members of the peasant community expressed their moral and philosophical yearnings, they described their conception of authority and sovereignty, they disclosed their fear of running short of bread, their dread of being struck by a disease or else their craving for culture. They wondered about the adequate means to collect the necessary funds for the education of their children, for medicine or to lure a capable surgeon and a competent midwife into settling in their village. Thus people of modest means cast a special light - at least in the context of a meeting - on what the ideals of the + siècle des Lumières ; were to lead on to on a local scale
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Rohe, Lena [Verfasser], Reinhard [Akademischer Betreuer] Well, Nicole [Akademischer Betreuer] Wrage-Mönnig, Klaus [Akademischer Betreuer] Dittert, and Heinz [Akademischer Betreuer] Flessa. "Nitrous oxide from fungal denitrification - Pure culture and soil studies using stable isotope and microbial inhibitor approaches / Lena Rohe. Gutachter: Nicole Wrage-Mönnig ; Klaus Dittert ; Heinz Flessa. Betreuer: Reinhard Well." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2014. http://d-nb.info/1064148298/34.
Повний текст джерела
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Garnier-Petit, Agathe. "Purification et caractérisation de la glutamate déshydrogénase à NAD chez le basidiomycète ectomycorhizien Laccaria bicolor : régulation de son activité chez le champignon en culture pure et dans deux associations ectomycorhiziennes." Nancy 1, 1998. http://www.theses.fr/1998NAN10312.
Повний текст джерелаАнотація:
La GDH à NAD du champignon ectomycorhizien Laccaria bicolor a été purifiée à homogénéité électrophorétique. Le protocole de purification comporte trois étapes simples, une précipitation au sulfate d'ammonium, une chromatographie échangeuse d'anions et une filtration moléculaire. La GDH à NAD a été purifiée 410 fois avec un rendement de 40%. La masse moléculaire de l'enzyme native est de 470 kDa, celle des sous-unités de 116 kDa suggérant que la GDH à NAD est un tétramère. L’enzyme est spécifique pour le NAD(H). Les pH optimums sont de 7,4 et de 8,8 pour les réactions d'amination et de désamination respectivement. L’addition de sulfate d'ammonium permet une bonne stabilité de l'activité enzymatique. La GDH à NAD est stimulée par le calcium, le magnésium, fortement inhibée par le cuivre et légèrement par les nucléotides AMP, ADP et ATP. Les constantes d'affinité pour le NAD, NADH, α-cétoglutarate et l'ammonium sont de 282 µM, 89 µM, 1,35 mM et 37 mM respectivement. Le rôle physiologique de la GDH à NAD a été étudié chez Laccaria bicolor cultive sur différentes sources azotées. Une régulation différente des deux GDHS est mise en évidence sur milieu nitrate et ammonium, conséquence de leur fonction métabolique distincte. La GDH à NAD et la GS ont un rôle assimilateur tandis que la GDH à NAD possède une fonction déprédative. La source et la concentration en azote régule fortement la GS et la GDH à NAD. Ces enzymes sont réprimées par les fortes teneurs en azote, en relation avec la teneur en ammonium intracellulaire. La GDH à NAD est nettement moins influencée par le statut azoté cellulaire. L’effet des sources carbonées sur les activités GDH à NAD et GDH à NADP caractérise une simulation de ces deux enzymes sur un milieu contenant du succinate et du fumarate. La GDH à NAD est plus fortement stimulée. Ceci suggère un contrôle de ces enzymes par le circuit carboné. L’étude de l’expression de la GDH à NAD dans deux ectomycorhizes montre une répression de l’isoforme fongique tandis que l’isoforme racinaire est conservée. Dans les ectomycorhizes de Douglas / Laccaria bicolor, la GDH à NADP fongique est active tandis que dans l’association Eucalyptus globulus / Laccaria bicolor, elle est réprimée.
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Cottrell, Corey Ann. "Projeto Pure Mutt - Puro vira lata: Um Estudo Coreográfico nas Danças Urbanas: Samba-reggae, Capoeira e Hip Hop." reponame:Repositório Institucional da UFBA, 2007. http://www.repositorio.ufba.br/ri/handle/ri/9213.
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Esta dissertação tem o objetivo de demonstrar o processo da criação coreográfica inspirada em danças urbanas da diáspora africana. Na pesquisa coreográfica, foram trabalhadas as seguintes modalidades de dança: samba-reggae, capoeira e hip hop. Um dos principais objetivos foi o trabalho integrado com os artistas-participantes. O projeto realizou a apresentação de um trabalho de criação coletiva, que foi gravado na forma digital e editado em DVD, no qual revela toda a pesquisa e o seu processo criativo, contendo depoimentos dos participantes, clips dos ensaios e a performance final. Os resultados do projeto foram obtidos através da pesquisa bibliográfica e da pesquisa de campo. A pesquisa bibliográfica focalizou nas danças urbanas acima mencionadas, as teorias de cultura, etnicidade, cultura popular afro-brasileira e afro-americana, e a globalização. A pesquisa de campo tomou forma a partir de colaborações com a Escola de Dança da Fundação Cultural do Estado da Bahia e da Escola de Capoeira Ginga e Malícia, e com a minha participação como artista e espectadora em alguns eventos de samba-reggae, capoeira, hip hop e reggae, em Salvador, durante o período de 2005 a 2007. Na Escola de Dança (FUNCEB), Escola de Capoeira Ginga e Malícia e na Faculdade de Educação na UFBA foram realizados ensaios durante três meses, que produziram o produto criativo alvo desta pesquisa. A apresentação final foi realizada e gravada na Praça Dois de Julho, no Campo Grande, em Salvador - BA, em dezembro de 2006. Este trabalho também foi apresentado na 32a Conferência da Associação de Estudos Caribenhos em Salvador, em maio de 2007.
Salvador
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Caris, Andrade Rodrigo. "Biodegration of commonly used plasticizers by pure bacterial cultures." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=106606.
Повний текст джерелаАнотація:
Di-(2-ethylhexyl) adipate, diethylene glycol dibenzoate and dipropylene glycol dibenzoate are commonly used plasticizers, but also known contaminants when they are released into the environment. Studies involving growing the common soil bacterium Rhodococcus rhodochrous and the yeast Rhodotorula rubra in the presence of the plasticizers and an additional carbon source have shown that these compounds are partially degraded, the breakdown products are toxic metabolites and some of them resist further degradation.In order to observe if the degradation pathway and the fate of the metabolites produced are similar in other microorganisms, and if the additional carbon source is needed for the degradation to occur, the plasticizers listed above were added to several different strains of common soil bacteria after they had been grown to the stationary phase and all of the hexadecane provided as a carbon source had been used.It was found that all of the selected bacteria were able to degrade the studied plasticizers, even though there was no additional carbon source available. Degradation was found to be much more extensive in the case of di-(2-ethylhexyl) adipate compared to the dibenzoates. The biodegradation pathway for these compounds was found to be the same as proposed in the previous work using an additional carbon source and the same metabolites were detected. In particular, the most toxic and recalcitrant metabolites (2-ethylhexanol, 2-ethylhexanoic acid, diethylene glycol benzoate and dipropylene glycol benzoate) were observed, and generally, accumulation occurred, although partial degradation was detected in some cases.This work proved that an additional carbon source is not needed for the biodegradation of the chosen plasticizers to happen, and that the biodegradabilities and pathways observed for Rhodococcus rhodochrous and Rhodotorula rubra is extensive to a wider range of common soil bacteria.L'adipate de di-(2-éthylhexyle), le dibenzoate de diéthylène-glycol et le dibenzoate de dipropylène-glycol sont des plastifiants d'usage commun identifiés comme contaminants environnementaux. Des études de biodégradation de ces plastifiants, effectuées avec la bactérie Rhodococcus rhodochrous et la levure Rhodotorula rubra en présence d'une source additionnelle de carbone, ont montré que ces molécules sont partiellement biodégradés. Cette biodégradation mène à la formation de métabolites toxiques, dont certains sont persistants. L'objectif de cette thèse de maitrise était de déterminer si le mécanisme de biodégradation et la nature des métabolites formés sont transférables à d'autres microorganismes et d'évaluer le rôle de la source additionnelle de carbone dans cette biodégradation. Pour ce faire, les plastifiants mentionnés ci-dessus ont été ajoutés à des cultures pures de différentes bactéries communément présente dans le sol, et ce, lorsque ces cultures ont atteint la phase stationnaire et que la source additionnelle de carbone, l'héxadécane, ait été totalement consommée. L'ensemble des bactéries étudiées a démontré un potentiel de biodégradation des plastifiants testés, et ce, même en absence d'une source additionnelle de carbone. Un niveau plus élevé de biodégradation a été observé dans le cas de l'adipate de (2-éthylhexyle) que dans ceux des dibenzoates. Les mécanismes de biodégradation observés ici en absence d'une source additionnelle de carbone sont comparables à ceux rapportés dans la littérature en présence d'héxadécane. Les métabolites formés étaient également les mêmes et bien que seulement une biodégradation partielle ait été observée, la formation des métabolites les plus récalcitrants et toxiques (2-éthylhexanol, acide 2-éthylhexanoïque, benzoate de diethylène glycol et benzoate de dipropylène glycol) a mené à une certaine accumulation.Cette thèse de maitrise démontre qu'une source additionnelle de carbone n'est pas nécessaire pour que la biodégradation de ces plastifiants ait lieu et que la biodégradabilité ainsi que les mécanismes de biodégradation publiés antérieurement ne sont pas spécifiques aux microorganismes Rhodococcus rhodochrous et Rhodotorula rubra et s'étendent plutôt à divers types commun de bactéries. Ces résultats suggèrent une biodégradation probable de ces plastifiants dans l'environnement.
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Cronje, Marise Christine. "Production of kepi grains using pure cultures as starters." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53561.
Повний текст джерелаАнотація:
Thesis (MSc Food Sc )--Stellenbosch University, 2003.ENGLISH ABSTRACT: Kepi is a refreshing, fermented dairy beverage that differs from other fermented milk products in that it is produced with a mixed microbial community which is confined to discrete grains. These grains can be recovered as a solid matrix at the end of the fermentation and then be reutilised as a starter to ferment the next batch of milk. The grain microbial community consists of a symbiotic association of yeasts and lactic acid bacteria, but the overall composition of the grains has not been completely elucidated. The microbes in the grains are embedded in a protein-polysaccharide Kefiran matrix, which appears essential for grain formation. The mechanism of grain formation is still not fully understood and it thus remains undecided which organism is really responsible for the production of this proteinpolysaccharide matrix. The aim of this study was to isolate, characterise and identify the microbes present in Kefiran from mass cultured South African grains and then to evaluate grain formation with these purified cultures isolated from Kefiran strings using a mass cultivation process. Sixteen strains of lactic acid bacteria and one yeast strain were isolated from Kefiran strings produced during the mass cultivation of South African Kepi grains. API technology, numerical clustering and DNA sequence comparisons were used to identify the purified isolates. The isolates were grouped into seven clusters by numerical clustering and clustering distance from selected reference and marker strains. The heterofermentative lactobacilli were identified as Lactobacillus parakefiri and Lb. kefiri and the homofermentative strains as Lb. delbrueckii ssp. bulgaricus, Lb. gallina rum, Lb. acidophilus and Lb. bavaricus. One isolate was found to be a member of the genus Lactobacillus, but was not positively identified to species level. Cultures isolated from Kefiran were evaluated for ability to grain formation by adding 1 x 109 cfu.ml:' bacteria and 1 x 108 cfu.ml' yeast to double pasteurised, full cream milk during the mass cultivation process. It was found that the control and all the cultures in double pasteurised milk showed grain accumulation indicating that other microbes were present in pasteurised and double pasteurised milk which had an influence on the grain forming ability. The cultures isolated from pasteurised and double pasteurised milk included members of the species Pediococcus, Acinetobacter, Lactococcus laetis ssp. lactis, Candida lipolytica, C. guilliermondii, Chryseobacterium meningosepticum, Pseudomonas putida and four isolates of the Bacillus cereus group. It was found that these rod-shaped "milk isolates" resulted in grain accumulation when inoculated into UHT milk and it was concluded that the "milk isolates" did contribute to grain formation. These isolates were then combined with the Kefiran cultures and this resulted in grains very similar to the traditional Kepi grains. These grains were made from Lb. gallinarum in double pasteurised milk as well with a combination of Lb. gallinarum, Lb. acidophilus, Lb. kefiri, Lb. delbrueckii ssp. bulgaricus, Candida lambica and Pseudomonas putida in URT milk. The grains were firm, elastic and did not dissolve in water but kept their structure and were retained when sieved. An acceptable Kepi beverage was produced from these grains. From these typically traditional grain characteristics it was concluded that, even though the microbial compositions were probably not the same, the general appearance was similar to traditional grains and that it is thus possible to produce grains from pure single strain Kefiran cultures and "milk isolates". Furthermore, it was possible to produce a Kepilike beverage from these grains, which included similar characteristics as the traditional Kepi beverage.
AFRIKAANSE OPSOMMING: Kepi is "n verfrissende, gefermenteerde suiweldrankie wat van ander gefermenteerde produkte verskil in die opsig dat dit vervaardig word deur Kepi korrels in melk te inkubeer. Die Kepi korrels kan aan die einde van die fermentasie herwin word en weer gebruik word om die volgende lot melk te fermenteer. Die korrels bestaan uit "n simbiotiese samestelling van giste en melksuurbakterieë, maar die presiese samestelling van die korrels is steeds onbekend. Die mikro-organismes is vasgevang in "n proteïen-polisakkaried Kefiran matriks en die Kefiran word as essensieel beskou vir korrelvorming. Die meganisme van korrelvorming bly steeds onbekend en daar is nog nie tot "n gevolgtrekking gekom oor watter organisme die Kefiran produseerder is nie. Die doel van die studie was om die mikro-organismes in Kefiran te isoleer en te identifiseer deur Suid-Afrikaanse Kepi korrels te massa kweek. Hierdie mikroorganismes was dan verder geëvalueer ten opsigte van korrel vorming. Sestien melksuurbakterieë isolate en een gis isolaat is geïsoleer vanuit die Kefiran. API tegnologie, numeriese groepering en DNA volgorde vergelykings was gebruik om die isolate te identifiseer. Die isolate is in sewe groepe verdeel volgens numeriese groepering. Die afstand van verwysings en merker organismes is ook in ag geneem. Die heterofermentatiewe organismes is geïdentifiseer as Lactobacillus parakefiri en Lb. kefiri en die heterofermentatiewe organismes as Lb. delbrueckii ssp. bulgaricus, Lb. gallina rum, Lb. acidophilus en Lb. bavaricus. Een isolaat kon nie geïdentifiseer word tot op spesie vlak nie, maar is verwant aan die genus Lactobacillus. Hierdie geïsoleerde Kefiran kulture is geëvalueer ten op sigte van korrelvorming, deur 1 x 109 kve.ml' van die bakterieë en 1 x 108 kve.ml' van die gis by dubbel gepasteuriseerde volroom melk te voeg tydens die massakwekings proses. Die kontrole wat geen bygevoegde kulture bevat nie, sowel as die wat wel bygevoegde kulture bevat, het korrel vorming getoon. Laasgenoemde toon dat daar organismes teenwoordig is in gepasteuriseerde en dubbel gepasteuriseerde melk wat "n rol kan speel tydens korrelvorming. Die kulture wat geïsoleer is vanuit gepasteuriseerde en dubbel gepasteuriseerde melk, sluit in: Pediococcus, Acinetobacter, Lactococcus laetis ssp. lactis, Candida lipolytica, C. guilliennondii, Chryseobacterium menigosepticum, Pseudomonas putida en vier isolate van die Bacillus cereus groep. Hierdie organismes wat uit melk geïsoleer is, het korrelvorming getoon in UHT melk en die gevolgtrekking kan gemaak word dat die "melk organismes" wel "n rol speel tydens korrel vorming. Hierdie "melk isolate" in kombinasie met die Kefiran kulture het korrels tot gevolg gehad wat baie dieselfde was as tradisionele Kepi korrels. Laasgenoemde korrels is gemaak deur Lb. gallina rum in dubbel gepasteuriseerde melk, sowel as deur "n kombinasie van Lb. gallina rum, Lb. acidophilus, Lb. kefiri, Lb. delbrueckii ssp. bulgaricus, Candida lambica en Pseudomonas putida in UHT melk. Die korrels was stewig, elasties, het nie opgelos in water nie en het hulle struktuur behou wanneer gesif. Wanneer hierdie tipiese tradisionele korrels se eienskappe in ag geneem word, kan die gevolgtrekking gemaak word dat alhoewel die mikrobiese samestelling van die korrels nie dieselfde is as die tradisionele korrel nie, is die algemene voorkoms en eienskappe dieselfde en dat dit wel moontlik is om korrels te produseer deur isolate geïsoleer vanuit Kefiran en melk. Verder was dit moontlik om "n drankie te vervaardig met die korrels wat baie dieselfde is as tradisionele Kepi.
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Besse, Laurent. "Les maisons des jeunes et de la culture : 1959-1981 : Etat, associations, municipalités." Paris 1, 2004. http://books.openedition.org/pur/4064.
Повний текст джерелаАнотація:
1959-1981 : de " l'été des blousons noirs " à " l'été des Minguettes ", cette étude retrace 1 'histoire des Maisons des jeunes et de la culture, principales institutions du secteur socio-culturel. Equipement, gestion associative et fédérative et professionnalisation des animateurs firent le succès de la " formule MJC ". Nées dans l'après-guerre, elles connurent leur essor avec la montée des jeunes des années 60. Une approche institutionnelle, - les politiques publiques de la jeunesse et de la culture, sous l' angle des rapports entre pouvoirs publics et associations - est complétée par une dimension sociale et culturelle : étude des modèles pédagogiques, de la culture diffusée, de la sociologie du public et des animateurs. L' évocation du Youth Service anglais complète cette étude, qui s'efforce de concilier une vision " par le haut " - les rapports avec l'Etat - et une vision " par le bas " - l'histoire des MJC dans des contextes locaux, notamment dans leurs relations avec les municipalités.
31
Lyons, Reneé C. "Celebrate Hispanic Culture with Pura Belpré Award Winners." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/2378.
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Krammer, Thibault. "Développement d'un réseau microvasculaire sur puce microfluidique pour la reconstruction tissulaire." Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAV044.
Повний текст джерелаАнотація:
L’ingénierie tissulaire vise à développer in vitro des tissus fonctionnels ou des organes afin de fournir des plateformes de tests de médicaments ou des tissus transplantables et améliorer les traitements fournis aux patients. Cependant, les constructions tissulaires physiologiques développées à ce jour n’intègrent pas un réseau vasculaire perfusable. In vivo, le réseau vasculaire approvisionne les cellules de l’organisme en oxygène et nutriments et évacue les déchets cellulaires et le dioxyde de carbone. Il possède également un rôle prépondérant dans le maintien de l’homéostasie des organes. L’approvisionnement des cellules s’effectue au niveau des capillaires sanguins : vaisseaux creux dont la paroi est uniquement composée d’une couche de cellules endothéliales. Le réseau de capillaires sanguins est un réseau dense perfusant l’ensemble des tissus de l’organisme. De par la limite de diffusion de l’oxygène dans les tissus, chaque cellule est située au maximum à 200 µm d’un capillaire. Les difficultés de construction d’un réseau de capillaires sanguins perfusable et d’intégration au sein de constructions tissulaires limitent le développement de tissus physiologiques épais.Une technique innovante de développement d’un réseau microvasculaire à l’intérieur d’une construction épaisse est présentée dans cette thèse. Cette technique consiste en l’assemblage de micro-unités tissulaires sphériques au sein d’une chambre microfluidique, et en le développement d’un réseau de capillaires au niveau des pores interstitiels formés par l’empilement de sphères. Les micro-unités tissulaires sont composées de biopolymères représentatifs de la matrice extracellulaire et contiennent des cellules du tissu d’intérêt. Une couche de cellules endothéliales est développée à la surface de ces microsphères. L’empilement de ces microsphères crée un milieu poreux dans lequel du milieu nutritif est perfusé. Le contrôle de l’écoulement au sein d’une telle structure permet l’application de stimuli physiques influençant l’auto-assemblage des cellules endothéliales en capillaires au sein de l’espace interstitiel de l’empilement.Durant cette thèse, un dispositif de fabrication de microsphères à partir de biopolymères naturels a été développé. La structure formée par les empilements de sphères a été étudiée et les écoulements au sein de tels milieux ont été caractérisés de sorte à appliquer des stimuli physiques contrôlés aux cellules. Un système microfluidique de perfusion, de type bioréacteur, intégrant une chambre de développement a été fabriquée. Une construction tissulaire épaisse a pu être formée au sein de ce système et le développement du réseau vasculaire a été favorisé. La formation du réseau a été montrée par la présence de capillaires sanguins perfusés au sein de la structure. La technique développée promet une application au développement de nombreux tissus et des applications pour des dispositifs d’organes-sur-puces ou d’ingénierie tissulaireTissue engineering aims to develop functional tissues or organs in vitro in order to provide drug testing platforms or transplantable tissues and improve the treatments provided to patients. However, the physiological tissue structures developed to date do not integrate and perfusable vascular network. In vivo, the vascular network supplies the body’s cells with oxygen and nutrients and removes cellular waste and carbon dioxide. It also has a major role in maintaining organ homeostasis. Blood capillaries are hollow vessels whose walls are only composed of a layer of endothelial cells and diffuse nutrients. The blood capillary network is dense and perfuse all tissues. Due to the limit oxygen diffusion inside tissues, each cell is located at most 200µm away from a capillary. The difficulties of building a network of perfusable capillaries and integrating them into tissue constructs limit the development of thick physiological tissues.An innovative technique for developing a microvascular network within a thick construction is presented in this thesis. This technique consists of assembling spherical tissue micro-units within a microfluidic chamber, and developing a network of capillaries through the interstitial pores formed by the spheres packing. Tissue micro-units are composed of biopolymers representative of the extracellular matrix and contain cells from the tissue of interest. A layer of endothelial cells is developed on the surface of these microspheres. The stacking of these microspheres creates a porous medium in which nutrient medium is perfused. Flow control within such a structure allows the application of physical stimuli influencing the self-assembly of endothelial cells into capillaries within the interstitial space of the sphere packing.During this thesis, a device for manufacturing microspheres from natural biopolymers was developed. The structure formed by the stacks of spheres was studied and the flow within such environments were characterized so as to apply controlled physical stimuli to cells. A bioreactor-like perfusion system has been built. A thick tissue structure could be formed within this system and the development of the vascular network was promoted. The formation of the network was demonstrated by the presence of infused blood capillaries within the structure. The technique developed promises to be applied to the development of many tissues and applications for organ-on-chip or tissue engineering devices
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Wilson, Craig Stewart. "The biodegradation of nitriles by pure and defined mixed cultures of bacteria." Thesis, University of Kent, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242890.
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Chuang, Adina Shiang Mattes Timothy E. "Proteomic investigations of vinyl chloride-assimilating bacteria from pure cultures to the environment /." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/347.
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Chuang, Adina Shiang. "Proteomic investigations of vinyl chloride-assimilating bacteria: from pure cultures to the environment." Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/347.
Повний текст джерелаАнотація:
Vinyl chloride (VC) is a common groundwater pollutant and known human carcinogen that is commonly produced from the incomplete reductive dechlorination of tetrachloroethene and trichloroethene, chlorinated solvents often used in plastics and dry cleaning solvent manufacturing. The treatment of VC-contaminated sites by bacteria that can biodegrade VC has been demonstrated to be a practical and potentially cost-effective alternative to traditional "pump and treat" site cleanup options. However, little is known about the biochemical pathways involved in VC-assimilation within these strains and their distribution and activity in situ in the environment. This work uses mass-spectrometry-based proteomics to contribute to the understanding of these microbial communities in both pure cultures and in the environment.
The biochemical pathways of VC and ethene oxidation in Nocardioides sp. strain JS614 were studied using proteins identified with a peptide mass fingerprinting approach. New insights into a previously proposed pathway were made using mass spectrometry (MS)-based protein identifications, and potential protein biomarkers for the presence and activity of VC-assimilating bacteria in the environment were identified.
Techniques to extract proteins from various environmental samples such as activated sludge, sediments, soils, and water samples were developed based on preliminary experiments with protein extraction from strain JS614. The results of these studies demonstrated the successful extraction and identification of proteins involved in VC-assimilation from ethene-enriched groundwater samples. The presence and diversity of VC-assimilating bacteria in several ethene-enriched groundwater samples were examined using tandem mass spectrometry analysis to identify the protein biomarkers EtnC and EtnE.
VC-assimilating organisms can evolve in vitro from bacteria that grow on ethene but very little is known about the molecular changes involved. Proteomic investigations comparing three strains of Mycobacterium strain JS623, a wild type and two VC-adapted strains, validated previous studies indicating that protein expression changes are involved in VC-adaptation. Tandem mass spectrometry and spectral counting were used to identify proteins and semi-quantitatively estimate protein expression levels in the three ethene-grown JS623 variants. The results of this study suggest that multiple VC-adaptation mechanisms are involved in the two VC-adapted strains
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Berthuy, Ophélie. "Puce à cellules multiplexée pour l'étude de réponses cellulaires parallélisées." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10133/document.
Повний текст джерелаАнотація:
Les travaux présentés dans cette thèse concernent le développement d'une puce à cellules multiplexée pour l'étude de réponses cellulaires parallélisées. La lignée de cellules issues de cancer de la prostate, les cellules LNCaP, ont servi de modèle d'étude grâce à leur capacité à sécréter l'antigène prostate-spécifique (PSA) et la β-2-microglobuline (B2M) en réponse à l'induction par des hormones telles que la dihydrotestostérone (DHT). Nous avons ainsi pu détecter en temps réel et sans marquage ces molécules lors de leur sécrétion par de petites populations de cellules LNCaP adhérentes (de 1 à 100 cellules) à des positions déterminées sur une biopuce SPRi. Trois approches différentes ont été envisagées pour cette biopuce. La première consistait à microtexturer la surface d'or d'un support de SPRi afin d'obtenir des micropuits dont le fond révèle la surface d'or (région cytophile) et dont l'extérieur est composé de polystyrène (cytophobe) afin de créer un contraste d'adhésion pour la culture cellulaire. Des anticorps ont pu être immobilisés de façon contrôlée dans les micropuits grâce à un automate de micro-dépôt non contact. Dans ce système miniaturisé, différentes lignées cellulaires ont pu être co-cultivées sur une surface de 1 cm², ouvrant la voie au multiplexage. Une petite population de cellules (de 1 à 100) a été déposée de façon automatisée dans chaque micropuits. Afin de maintenir les cellules dans un milieu hydraté au cours du dépôt, un polymère d'alginate biocompatible a été utilisé. Cette méthode permet l'encapsulation de cellules dans un très petit volume (< 50 nL). La capacité de cet hydrogel à maintenir les cellules encapsulées à une position donnée sur le support a conduit à la conception d'une deuxième approche pour la fabrication de la biopuce. En effet dans cette approche la surface n'est pas modifiée et les composés biologiques (anticorps et cellules) sont directement déposés de façon automatisée sur la couche d'or. Enfin une dernière approche a été développée en immobilisant cette fois-ci les cellules sur un support microtexturé placé en face de la couche sensible de SPRi. Dans les trois approches, les cinétiques de sécrétion du PSA et de la B2M sécrétées ont pu être suivies par SPRiThe work reported in this thesis focuses on the development of a multiplexed cell chip for the study of parallelized cellular responses. The lineage of cells from Prostate cancer LNCaP cells, were used as a study model thanks to their ability to secrete prostate-specific antigen (PSA) and β-2-microglobulin (B2M) in response to induction by hormones such as dihydrotestosterone (DHT). We were able to detect in real time these label-free molecules and their secretion by small populations of adherent LNCaP cells (from 1 to 100 cells) at specified positions on a SPRi biochip. Three different approaches were considered for this biochip. The first was to pattern the gold surface of a SPRi slide to obtain microwells whose bottom reveals the gold surface (cytophilic area) and an outer shell composed of polystyrene (cytophobe) to create an adhesive/non-adhesive surface for cell culture. Antibodies were immobilized in a controlled manner in the microwells using a piezo electric spotter. In this miniaturized system, different cell lines were co-cultured on a surface of 1 cm², paving the way for multiplexing. A small population of cells (1 to 100) was deposited in an automated manner into each microwell. In order to maintain the cells in a hydrated environment during deposition, a biocompatible alginate polymer was used. This method allows the encapsulation of cells in a very small volume (<50 nL). The ability of the hydrogel to maintain the encapsulated cells in a given position on the support led to the design of a second approach for the production of the biochip. In this second approach the surface is not altered and biological compounds (antibodies and cells) are directly deposited in an automated manner on the gold layer. Finally, a last approach was developed by immobilizing the cells on a patterned substrate placed in front of the sensitive layer SPRi. In all three approaches, the kinetics of PSA secretion and secreted B2M could be followed by SPRi
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Dastidar, Aniruddha. "ARSENITE OXIDATION BY PURE CULTURES OF THIOMONAS ARSENIVORANS STRAIN B6 IN BIOREACTOR SYSTEMS." UKnowledge, 2010. http://uknowledge.uky.edu/gradschool_diss/70.
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The removal of arsenic toxicity from water is accomplished by a preliminary preoxidative step transforming the most toxic form, arsenite (As (III)), to the least toxic form, arsenate (As (V)). The potential of As (III) oxidation to As (V) was initially investigated in batch reactors using the chemoautotrophic Thiomonas arsenivorans strain b6 under varying initial As (III) and cell concentrations and at optimal pH and temperature conditions (pH 6.0 and temperature 30°C). The strain b6 completely oxidized As (III) to As (V) during exponential growth phase for lower levels of As (III) concentrations (≤ 100 mg/L) but continued into stationary phase of growth for higher levels (≥ 500 mg/L). Other important factors such as oxygen and carbon limitations during biological As (III) oxidation were also evaluated. The biokinetic parameters of the strain b6 were estimated using a Haldanesubstrate inhibition model with the aid of a non-linear estimation technique.
Microbial As (III) oxidation was further investigated in continuous-flow bioreactors (CSTR and biofilm reactor) under varying As (III) loading rates. Both the reactors achieved As (III) oxidation efficiency exceeding 99% during the steady-state conditions. The reactors were also able to recover from an As (III) overloading phase establishing the resilient nature of the microorganism. The basic mass balance expressions on As (III) and biomass along with the Monod model were used to linearly estimate the biokinetic parameters in the CSTR study. However, in the biofilm study, a steady-state flux model was used to estimate the same parameters. The performance of the model was very good in simulating the transient and steady-state conditions.
Finally, the potential application of one-stage and two-stage reactor systems was investigated for the near complete removal of arsenic. Activated alumina was used as the adsorbent for the As (V) produced by the biological oxidation of As (III). The two-stage reactor process performed better than the one-stage reactor system in lowering the arsenic level below the detection limit (1 mg/L) for at least eight days of operation. However, pH fluctuations and probable competition from ions such as PO43- , SO42-, and Cl- severely impacted the performance of the reactors. Further study is needed to improve the overall efficiency of the reactor systems for achieving complete removal of arsenic for a longer operating time.
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Estevam, Andressa. "Obtenção de BIOH 2 a partir da fermentação anaeróbia de efluente de cervejaria utilizando cultura pura isolada do ambiente." Universidade Estadual do Oeste do Paraná, 2017. http://tede.unioeste.br/handle/tede/3009.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The search for alternative fuels to replace the current demand for fossil fuels is growing worldwide, as is the case of the use of biomass for the production of clean energy. H2, besides being an important input in the industrial sector, is an excellent fuel due to its calorific value and the non-generation of gaseous pollutants. The production of hydrogen by anaerobic fermentation has been highlighted as an important route of production because it allows the use of residual substrates by microorganisms producing H2. Such microorganisms can be found in many sources in the form of anaerobic microbial consortia, for use in the form of mixed culture or for isolation of species. This work aimed at the isolation of bacteria from the environment, with the capacity to produce hydrogen by anaerobic fermentation of different substrates and the application of these cultures to the production of H2 using as a source of carbon the brewery effluent. Strains of bacteria isolated from avian litter were characterized and tested for potential H2 production in small scale trials, in static bottles and different substrates, in mesophilic condition. From the selection of a strain of better performance, the experiments were carried out in a mechanically stirred reactor, in a batch of 72 hours and using exclusively the brewery effluent. The H2 production occurred in all the tests and the average biogas flow rate was 72 mL h-1, H2 H2 molar flow rate of 1.2 mmol H2 h-1 and H2 content in the biogas of 30 to 40%, being this compound only by CO2 and H2.
A busca por combustíveis alternativos para substituir a demanda atual de combustíveis fósseis vem crescendo mundialmente, como é o caso da utilização de biomassa para a produção de energia limpa. O H2, além de ser importante insumo no setor industrial é um excelente combustível devido a seu poder calorífico e à não geração de gases poluentes. A produção de hidrogênio por fermentação anaeróbia vem se destacando como uma importante rota de produção pois permite a utilização de substratos residuais por micro-organismos produtores de H2. Tais micro-organismos podem ser encontrados em muitas fontes na forma de consórcios microbianos anaeróbios, para uso na forma de cultura mista ou para isolamento de espécies. Este trabalho teve como objetivo o isolamento de bactérias do ambiente, com capacidade de produzir hidrogênio por fermentação anaeróbia de diferentes substratos e a aplicação destas culturas à produção de H2 utilizando como fonte de carbono o efluente de cervejaria. Cepas de bactérias isoladas de cama de aviário foram caracterizadas e testadas quanto ao potencial de produção de H2 em ensaios de pequena escala, em frascos estáticos e diferentes substratos, em condição mesofílica. A partir da seleção de uma cepa de melhor performance foram conduzidos ensaios em reator agitado mecanicamente, em batelada com duração de 72 horas e utilizando como fonte de carbono efluente de cervejaria, exclusivamente. A produção de H2 ocorreu em todos os ensaios sendo obtidos os valores médios de vazão de biogás de 72 mL h-1, vazão molar de H2 de 1,2 mmol H2 h-1 e teor de H2 no biogás de 30 a 40%, sendo este composto somente por CO2 e H2. PALAVRAS-
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Ibarra, Barrera María Carolina. "Plan de regeneración pueblo de Putre." Tesis, Universidad de Chile, 2013. http://www.repositorio.uchile.cl/handle/2250/116281.
Повний текст джерелаАнотація:
ArquitectoLa presente memoria constituye la síntesis de los conceptos abordados para el desarrollo del proyecto de título definido como “Plan de Regeneración poblado de Putre”, realizado durante el período académico 2012-2013. Durante estos ultimos años conceptos como Cultura, Identidad y Memoria han sido los principales gestores al momento de idear y construir nuevas formas de hacer y pensar la arquitectura. la oportunidad para desarrollar teóricamente esta linea de pensamiento converge durante el 5º año de carrera, principalmente durante la etapa de seminario, el cual llevó por nombre “Reconstrucción de un Paisaje Cultural”, es en este momento donde logre comprender que estos conceptos toman medida solo en su estrecha relacion con el territorio. Asumiendo que los procesos son diversos y nunca totalmente acabados, el proyecto presente simplemente esboza aquellas ideas, intenciones, intereses y compromisos que de modo personal me interesa desarrollar en el futuro. A continuación graficare los principales conceptos abordados para la elaboración del presente proyecto.
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Mosteller, Tracy M. "Sanitizer efficacy towards attached bacteria in a simulated milk pipeline system using pure and mixed cultures." Diss., This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-08062007-094410/.
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Laplace, Jean-Marie. "Cultures microbiennes pures et mixtes : production d'éthanol à partir des pentoses et des héxoses dérivés des lignocelluloses." Montpellier 2, 1992. http://www.theses.fr/1992MON20155.
Повний текст джерелаАнотація:
Quatre souches de microorganismes ont ete retenues a priori pour etudier les fermentations alcooliques du glucose et du xylose derives de lignocelluloses par cultures pures et par cultures mixtes. Dans le cadre des cultures pures, a partir de 60 g/l de xylose, un rendement alcoolique de 0,40 g/g et une productivite de 1,55 g/l. H ont ete obtenus avec pichia stipitis en reacteur continu a haute densite cellulaire. Les valeurs relatives a la conversion alcoolique du glucose (140 g/l) par zymomonas mobilis sont un rendement de 0,47 g/g, une productivite de 19,5 g/l. H et un taux de conversion du substrat de 99 p. Cent. Les resultats ont ete valides sur hydrolysats hemicellulosique et cellulosique de bois de peuplier. La mise en uvre de cultures mixtes a haute densite cellulaire en conditions microaerobies avec un mutant deficient respiratoire de saccharomyces diastaticus et pichia stipitis a permis la production d'ethanol a partir du melange glucose/xylose (50 g/l) avec un rendement de 0,41 g/g, une productivite de 3,1 g/l. H et un taux de conversion du substrat de 100 p. Cent. La fermentation alcoolique d'un hydrolysat total de bois de peuplier par culture mixte conduit a un rendement de 0,25 g/g, une productivite de 0,93 g/l. H avec une assimilation complete du substrat
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Baudoin, Régis. "Développement et caractérisation d'une puce à cellules pour le criblage d'agents toxiques." Compiègne, 2008. http://www.theses.fr/2008COMP1785.
Повний текст джерелаАнотація:
Les développements actuels liés à l’ingénierie tissulaire et aux microtechnologies permettent aujourd’hui de proposer de nouveaux outils de criblages in vitro pour les études de toxicité. Nous proposons de développer une biopuce à cellules mimant un organe in vitro. Afin de valider notre approche, nous présentons l’influence du microenvironnement sur la culture de lignées cellulaires rénales (MDCK) et hépatiques (HepG2/C3A). Dans cette étude, nous avons testé trois débits (0, 10 et 25 µL/min) et trois ensemencements cellulaires. Enfin, nous avons soumis notre biopuce à trois chargements de chlorure d’ammonium (0, 5 et 10 mM) afin de démontrer le potentiel de ce modèle pour de futures applications liées à la toxicité. L’activité cellulaire en biopuce a été suivie par la prolifération des cellules, les consommations de glucose et de glutamine, les productions d’albumine et d’ammoniac et enfin, par l’activité enzymatique de détoxification des CYP 1A. En condition dynamique, il a été observé une augmentation des consommations et des productions cellulaires au regard des conditions statiques. L’activité de détoxification des CYP 1A a été également accrue. En présence du chlorure d’ammonium les réponses cellulaires furent similaires en biopuce au regard des conditions de culture standard en Pétri. De plus, le chlorure d’ammonium a semblé induire l’activité des CYP 1A en biopuce. Par cette étude, nous montrons la pertinence de notre biopuce pour des tests de toxicité in vitro en condition dynamique. Ce nouveau modèle de culture cellulaire in vitro pourra à terme être applicable aux études de criblages dans les industries chimiques, pharmaceutiques et cosmétiquesCurrent developments in tissue engineering and microtechnology fields allowed proposing new pertinent tools to investigate in vitro toxicity. We propose the development of a cellular microchip that mimics organs in vitro. To validate our approach, we showed the pertinence of the microchip environment with the culture of a renal cell line, the Madin Darby Canine Kidney (MDCK) and a human hepatic cell line, the HepG2/C3A. In this study, we tested three flow rates (0, 10 and 25 µL/min) at three inoculated cell densities. Then, we tested our microchip with three ammonium chloride loadings (0, 5 and 10mM) in order to demonstrate its potential for future toxicity experiments. The cellular activities were monitored by the cell proliferation rates, the glucose and glutamine consumptions, albumin and ammonia productions and by the detoxication via the CYP 1A activity. In dynamic condition, the cellular activities in term of consumptions and productions were higher in the microchip than in static conditions. More especially, the detoxication activity of the CYP 1A was found higher. The toxicity analysis with the chloride ammonium showed similar tendencies in the microchip and in Petri standard culture conditions (reduction of proliferation. However, the ammonium chloride seemed induce a higher CYP 1A activity in the microchip. By these investigations, we showed the pertinence of the utilization of our microchip for in vitro dynamic toxicity testing. The targeted industries of this new in vitro cell culture model are the chemical, pharmaceutical and cosmetic industries
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MARIN, DOUGLAS. "Ecophysiologie de canavalia ensiformis (l. )dc. , en cultures pures et associees." Rennes 1, 1994. http://www.theses.fr/1994REN10196.
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Le developpement de la culture de canavalia ensiformis (l. )dc. , peut avoir un grand interet pour l'agriculture venezuelienne future, car il s'agit d'une espece riche en azote, tres tolerante aux conditions limitantes de climat et de sol, avec des rendements en matiere seche totale et en grains superieurs a ceux des autres legumineuses traditionnellement employees pour l'alimentation du betail. Canavalia pourrait se substituer partiellement a l'importation de soja pour l'elaboration des aliments concentres pour les animaux. Cette etude contribue a fournir des renseignements sur l'ecophysiologie de canavalia en culture pure et associee. Le travail utilise une demarche qui combine l'analyse de croissance et les observations phenologiques tout au long des cycles, ainsi que des mesures ponctuelles de la biomasse de mauvaises herbes, des variables liees au statut hydrique, a l'alimentation azotee et a l'interception de la lumiere ; en plus figure l'evaluation des rendements en grains avec comparaison des varietes en culture pure, et de l'espece associee a certaines plantes en c3 ou c4, economiquement importantes au venezuela
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Barbosa, Willer Araujo. "Cultura Purí e educação popular no município de Araponga, Minas Gerais." Florianópolis, SC, 2005. http://repositorio.ufsc.br/handle/123456789/102780.
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Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências da Educação. Programa de Pós-Graduação em Educação.Made available in DSpace on 2013-07-16T01:54:12Z (GMT). No. of bitstreams: 0
Análise do processo variável da emergência étnica no entorno do Parque Estadual da Serra do Brigadeiro na Zona da Mata de Minas Gerais. A Cultura Puri foi silenciada durante os últimos duzentos anos, a partir da Educação Popular, seu processo intercultural possibilitou a recriação de identidades. Através de entrevistas, de rodas de narração de histórias e das notas de campo em comunidades rurais recupera fontes orais. Capta um encadeamento discursivo recorrente entre violência, fabulações naturalísticas e vontade de liberdade. Se inscreve na tradição da crítica cultural como motor da transformação social. Associa-se à saga colonial migratória, e recoloca a temática dos movimentos sociais. Critica o monoculturalismo do saber através da inteligibilidade dos movimentos sociais e da ecologia de saberes. Enfatiza a constituição de uma outra temporalidade e da dilatação do tempo presente, o que aprofunda as análises de longa duração. Aborda conceitos que se interrelacionam complexamente, tais como: estrutura de sentimentos, hegemonia, residual e emergencial. Toma como referências os estudos de Raymond Williams e de Boaventura de Sousa Santos.
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Ferreira, Maria de Fatima. "Melancolia: da identificação narcísica à pura cultura da pulsão de morte." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/VCSA-6X3REP.
Повний текст джерелаАнотація:
Esta pesquisa visa elucidar o lugar que a melancolia ocupa na clínica psicanalítica, do ponto de vista estrutural. Ganha seu assento definitivo a partir de questões provenientes da dificuldade em estabelecer o diagnóstico diferencial intrapsicoses, pois às vezes, a melancolia se apresenta sem os fenômenos típicos da psicose, entre eles o delírio. No entanto, sem alguns casos sobressai o fenômeno de auto-acusação e das passagens ao ato suicída, onde se observa uma preponderância da pulsão de morte, sem nenhuma mediação fálica. Seguindo essa indicação é que se justifica investigar quais são os principais fenômenos específicos da melancolia, bem como a maneira pela qual esses fenômenos se manifestam nessa categoria clínica. Na pesquisa empreendida pela psiquiatria clássica, se destaca o fenômeno de auto-acusação, o delírio de negação, a dor moral e o delírio de indignidade como próprios da melancolia. A partir daí, podemos ver o quanto Freud buscou, na psiquiatria clássica, as principais idéias que sustentarão sua investigação acerca da melancolia, sobretudo em seus primeiros rascunhos. A investigação freudiana a respeito do desenvolvimento da teoria da libido, da concepção sobre o narcisismo e sobre a identificação, abre caminho para os pressupostos essenciais que sustentam seu célebre texto "Luto e melancolia". Neste, pode-se depurar três tipos clínicos: o luto normal, o luto patológico e a melancolia. É, pois, o mundo como o sujeito responde à perda de objeto que o direciona para uma dessas saídas. Ao considerar a identificação ao objeto perdido como específica melancolia, Freud avança sem sua investigação e chega, em 1924, a formular sua hipótese de que a melancolia é uma psiconeurose narcisista. Ressalta-se a concepção da identificação narcísica do melancólico ao objeto perdido como sendo o elemento-chave para elucidar essa questão. Assim ao estabelecer a distinção entre o luto e a melancolia, bem como entre a melancolia e a paranóia, avança-se na investigação acerca dos principais fatores que sustentam a concepção lacaniana da melancolia pertencer à estrutura da psicose, por se tratar, nesse caso, de uma identificação do tipo narcisista onde o melancólico está totalmente refernciado ao objeto a. Por fim, a partir dos fenômenos destacados como sendo próprios da melancolia, pode-se delimitar a posição particular do sujeito melancólico diante do Outro, bem como os efeitos mortíferos da identificação narcísica para o melancólico.
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Marques, Nara Eliza. "De Chocolat : um Griot à brasileira: o mais puro cacau da Bahia contando histórias na República do Café com Leite /." Araraquara, 2020. http://hdl.handle.net/11449/192553.
Повний текст джерелаАнотація:
Orientador: Dagoberto José FonsecaResumo: O final do século XIX e o começo do século XX é um período de intensa e diversa produção cultural decorrente das sucessivas mudanças que aconteciam não só no Brasil, mas em todo o mundo. Havia um processo de construção e afirmação de identidades e de reconstrução de hierarquias sociais que haviam ruído com a abolição do escravismo. Muitos desses movimentos culturais, principalmente os de agência de negros libertos, sofreram sucessivas tentativas de apagamento por terem caráter popular, ou seja, não se enquadrarem em um modelo europeu-ocidental reformador de costumes. É de um desses movimentos, mais especificamente de uma figura atuante na cena cultural do início do século XX que essa pesquisa irá erguer a voz. O nome artístico dele era De Chocolat, fundador da primeira companhia de teatro do Brasil composta apenas por negros, a Companhia Negra de Revista. Ele produz um teatro com uma importante função social. Utiliza-se de ferramentas populares como a música, a oralidade, a dança, os improvisos, os chistes, as anedotas para fazer críticas à sociedade da época. Essa dissertação terá como elemento central o fac-símile da primeira peça encenada por De Chocolat e sua companhia, intitulada “Tudo Preto”. É através desse material que será visto, que assim como um griot africano – que são intermediadores sociais e guardadores das histórias de seu povo – De Chocolat usará as populares Revistas Teatrais para contar a história da construção da identidade negra no Brasil tangenciando ass... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The end of the nineteenth century and the beginning of the twentieth century is a period of intense and diverse cultural production resulting from successive changes that took place not only in Brazil, but also around the world. There was a process of building and affirming identities and rebuilding social hierarchies that had broken with the abolition of slavery. Many of these cultural movements, especially those of freed black agencies, have suffered successive attempts to erase because they are popular in nature that is not fitting into a Western-European customs reformer. It is from one of these movements, more specifically of an active figure in the cultural scene of the early twentieth century that this research will raise the voice. His stage name was De Chocolat, founder of Brazil's first black theater company, Black Company of Magazines. He produces a theater with an important social function. It uses popular tools such as music, orality, dance, improvisations, jokes, anecdotes to criticize the society of the time. This dissertation will have as its central element the facsimile of the first play staged by De Chocolat and his company, entitled "All black". It is through this material that he will be seen, as well as an African griot – who are social mediators and keepers of the stories of his people – De Chocolat will use the popular Theatre of Magazines to tell the story of the construction of black identity in Brazil such as black women, racism, religiosity, Brazil... (Complete abstract click electronic access below)
Mestre
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Vadelorge, Loïc. "Pour une histoire culturelle du local : Rouen, 1919-1940." Paris 4, 1996. http://books.openedition.org/pur/10987.
Повний текст джерелаАнотація:
Si l'histoire culturelle de la france contemporaine dispose aujourd'hui de synthèses importantes, on connaît encore mal les conditions de developpement d'une vie culturelle locale. L'exemple de la ville de Rouen, étudiée dans l'entre-deux-guerres, suggère que des pratiques culturelles locales conséquentes ont pu exister à cette epoque. La vie culturelle d'une grande ville de province sous la troisième république s'articule autour d'un système complet d'institutions culturelles (musées, bibliothèques, théâtres) et se fonde sur une sociabilité efficace (sociétés savantes, sociétés musicales). Elle est confortée par l'émergence des politiques culturelles publiques qui concurrencent dès l'entre-deux-guerres, les formes traditionnelles d'encadrement de la culture (église, élites sociales). La culture locale paraît d'autant plus cohérente et apte à résister aux mutations économiques (inflation des années vingt, crise des années trente) ou culturelles (avènement de la culture de masse) qu'elle s'assigne un sens patrimonial, impulse à la fois localement et nationalement. La dynamique d'ouverture du champ culturel caractéristique du front populaire, touche finalement assez peu la ville, signe de la stabilité du schéma culturel local dans le premier XXe siecleIf french cultural history is today well known, the local culture development is still a matter of investigation. The case of Rouen, one of the most important provincial cities during the french twentieth century, suggest that local cultural practices have been in existence for a long time. The local culture during the third republic took one's stand on a complete system of cultural institutions (museums, libraries, theaters) and used all the resources of associations (scientist or musical societies). The birth of cultural policies, who compete with traditionnal forms of cultural organisations (clerical, elites) reinfor ce the local culture. It appears then able to resist to the economics mutations (inflation during the twenties, great crisis in 1929) or cultural mutations (the age of the masses). The patrimonial sense is here decisive as it result s of two impulsions, local and national. The overture of cultural sense, during the french popular front, will not be able to change the politics and the practices ; this is the sign of the extraordinary permanence of the local cultural system in the early twentieth century france
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Ratau, Mmaphuti Abashone. "Chemometrics, physicochemical and sensory characteristics of pearl millet beverage produced with bioburden lactic acid bacteria pure cultures." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2773.
Повний текст джерелаАнотація:
Thesis (MTech (Food Technology))--Cape Peninsula University of Technology, 2018.The aim of this study was to evaluate the physical, chemical and sensory characteristics of non-alcoholic pearl millet beverage produced using isolated and purified cultures of bioburden lactic acid bacteria (LAB). Traditional non-alcoholic pearl millet beverage (TNAPMB) was produced through spontaneous fermentation. The slurry was fermented for 36 h at 37°C while monitoring the microbial growth at 3 h interval. LAB were grown on deMan, Rogosa and Sharpe agar and identified using Vitek 2 system. The initial numbers of LAB were 7.04 log cfu/ml and increased to 8.00 log cfu/ml after 21 h. The beverage was dominated by LAB and contaminants and their survival was in succession. LAB from the genera Leuconostoc, Pediococcus, Streptococcus and Enterococcus were the main fermenting species in TNAPMB. Pearl millet extract (PME) was produced by hydrating pearl millet flour (PMF) with water (1:10, PMF:Water). To the mixture sprouted rice flour (10%), ground ginger (10%) and pectin (0.6%) were added. Stable PME was used in the production of plain non-alcoholic pearl millet beverage (PNAPMB). PME was pasteurized at 98°C for 30 min, hot filled and cooled to 25°C. The fluid was inoculated with Leuconostoc mesenteroides, Pediococcus pentosaceus and Enterococcus gallinarum each at 0.05, 0.075 and 0.1%, respectively, using factorial design and fermented for 18 h at 37°C. The pH of the beverage ranged between pH 3.32 and pH 3.90. L. mesenteroides, P. pentosaceus, E. gallinarum, the interaction between L. mesenteroides and P. pentosaceus and the interaction between L. mesentoroides and E. gallinarum had a significant effect (p ˂ 0.05) on the pH of PNAPMB except the interaction between P. pentosaceus and E. gallinarum (p = 0.631). The total titratable acidity (TTA) of the beverage ranged from 0.50 to 0.72%. All cultures had a significant influence (p ˂ 0.05) on the TTA of the beverage with the exception of the interaction between L. mesenteroides and E. gallinarum (p = 0.102). However, Monte Carlo simulation showed that E. gallinarum caused an increase in the pH and a decrease in the TTA of the beverage. During fermentation, the pH of the beverage is desired to decrease while the TTA increases, hence E. gallinarum was removed. The interaction between L. mesenteroides and P. pentosaceus at 0.05% and 0.025%, respectively produced an acceptable PNAPMB with potential for commercialization. Furthermore, moringa supplemented non-alcoholic pearl millet beverage (MSNAPMB) was produced by adding 4% of moringa (Moringa oleifera) leaf powder extract during the production of PNAPMB. The physicochemical, nutritional, microbial (LAB) and sensory characteristics of the PNAPMB, MSNAPMB and TNAPMB were determined. LAB were significantly (p < 0.05) affected by the fermentation period and increased from 3.32 to 7.97 log cfu/ml and 3.58 to 8.38 log cfu/ml in PNAPMB and SNAPMB, respectively. The pH of PNAPMB decreased from pH 5.05 to pH 4.14 while the pH of MSNAPMB decreased from pH 5.05 to pH 3.65 during the 18 h fermentation. The growth of LAB during fermentation had a significant effect (p < 0.05) on the pH of the beverages. The TTA increased from 0.14 to 0.22% and increased from 0.17 to 0.38%, in PNAPMB and MSNAPMB, respectively. The TTA of the beverage was affected significantly (p < 0.05) by the 18 h of fermentation. The protein content was 1.62, 2.17 and 1.50% in PNAPMB, MSNAPMB and TNAPMB, respectively. PNAPMB sample was deemed acceptable in comparison to the MSNAPMB. The total colour difference (ΔE) was 5.91 and 10.60 in PNAPMB and MSNAPMB, respectively in comparison to the TNAPMB. Volatile compounds with beneficial effect such as anti-inflammatory and anti-pathogenic properties were identified in the beverages. Principal component analysis indicated that the variations in characteristics of PNAPMB and MSNAPMB could be explained using total fat, saturated fat, total sugar, ash, moisture, proteins, chroma (C), hue and b*. The results showed that isolated pure cultures could be used as starter cultures in the production of non-alcoholic cereal beverages at a commercial level with predictable quality and safety properties.
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Gage, Sue-Je Lee. "Pure mixed blood the multiple identities of Amerasians in South Korea /." [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3253643.
Повний текст джерелаАнотація:
Thesis (Ph.D.)--Indiana University, Dept. of Anthropology, 2007.Title from PDF t.p. (viewed Nov. 19, 2008). Source: Dissertation Abstracts International, Volume: 68-02, Section: A, page: 0619. Adviser: Anya Peterson Royce.
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Platz, Teresa Katharina. "Café culture : socio-historical transformations of space, personhood and middle class in Pune, India." Thesis, Durham University, 2012. http://etheses.dur.ac.uk/4461/.
Повний текст джерелаАнотація:
Café Culture is an ethnographic snapshot, taken in 2008, tracing the effects of globalisation from the perspective of young middle class urbanites in post-liberalisation Pune, India. It captures what was happening that sets this young generation apart – the first to grow up in post-liberalisation India – as a group in historical time, in relation to other life worlds in India, to 'Western' versions and as a rounded life world in itself. In 1991 India conclusively opened its economy to the global market economy. My ethnography shows that trends following economic liberalisation in unprecedented ways spurred changes that were already underway. It facilitated not only the emergence of a commodified leisure culture in the form of cafés, targeted at and appropriated by the young urban middle class, but also the creation of new fashions, more living space, national and international employment, mobility and economic independence. These tangible changes went hand in hand with transformations in practices and moral aesthetic standards. The young generation was challenging their parents' and wider society's values in order to negotiate who they wanted and felt they ought to be in their rapidly changing world. In their friendships, café culture activities, fashion choices, education and love lives they increasingly valued, encouraged and expected equality, freedom and the expression of individuality. However, the different chapters highlight that these trends were measured and limited by class- and generation-based practices and moral aesthetic standards which amended rather than negated older patriarchal arrangements predicated on the ideal of joint family life. The young café culture crowd was negotiating to follow their hearts, while preserving strong family bonds and inter-generational dependencies. They were thus modifying what it meant to be middle class Indians in our contemporary world of flow of people, capital, ideas, images, information and goods.