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Hamyeh, Mohamed. "Régulation de l'agressivité tumorale mammaire par la protéine tyrosine phosphatase PTPL1/PTPN13." Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT015/document.
Повний текст джерелаThe regulation of breast tumor aggressiveness by Protein Tyrosine Phosphatase PTPL1/ PTPN13Breast cancer is a major problem for public health of which the incidence continues to increase. Its mortality is often linked to metastasis formation. Studies on PTPL1, the largest protein tyrosine phosphatase, have shown that it presents the characteristics of a tumor suppressor gene. PTPL1 is mutated in several types of cancers and its expression is associated with good prognostic in prostate and breast cancers. My team has shown that PTPL1 mediates the pro apoptotic effect of anti-estrogen in hormone-sensitive tumor cells by dephosphorylating IRS1, Insulin growth factor-1 receptor substrate, thus blocking PI3K/Akt pathway. In addition, PTPL1 regulates the growth, the invasion, and the adhesion of low aggressive breast tumor cells MCF-7.Our team established an isogenic cellular model capable of expressing PTPL1 or its mutants (phosphatase-dead and substrate-trapping mutants) in an inducible fashion in invasive cells. We showed that functional PTPL1 expression has a negative impact on cell aggressive phenotypes. Interestingly, the phosphatase-dead mutant exhibits the same behavior as the transfection control. This evidences that PTPL1 activity is crucial for the inhibition of aggressiveness. We are currently testing the clones tumorigenicity in athyemic mice.Furthermore, we conducted a comparative proteomic (SILAC) in order to study the global tyrosine phosphatome in MCF-7 and MDA-MB-231 cells with or without PTPL1. Our findings suggest that PTPL1 regulates the phosphorylation of proteins involved in different signaling pathways already described in the literature to be impacted by PTPL1. Remarkably, the quarter of proteins identified belong to cell junction structure or regulation. We then studied the impact of this phosphatase on cell junctions and showed that PTPL1 overexpression enhances cell aggregate formation in 3D culture, increases cell contact stability, relocates desmoglein to the cell junctions, and induces E-cadherin re-expression at the level of cell-cell contacts in MDA-MB-231 cells.Cell junctions and polarity are very important in oncology and particularly in the invasive process which is the first step in the metastatic dissemination. Our ongoing work focuses on identifying direct substrates for PTPL1 in order to elucidate the underlying PTPL1 signal leading to cell junctions and consequently propose a novel therapeutic targets
Dromard, Mathilde. "Rôles et mécanismes d'action de la protéine tyrosine phosphatase PTPL1/ptpn13 dans les cancers mammaires et ovariens." Montpellier 2, 2008. http://www.theses.fr/2008MON20040.
Повний текст джерелаRecent clinical studies have suggested an anti-oncogenic role for the Protein Tyrosine Phosphatase (PTP) PTPL1/ptpn13. Analysis of the inhibitory effect of 4-hydroxytamoxifen (OH-Tam) on growth factor action has allowed to identify the key role played by a new class of enzymes, PTPs, and more specially PTPL1 on the control of cell proliferation. The first part of my PhD studies, has demonstrated that PTPL1 inhibits activation of the Insulin Receptor Substrate-1 (IRS-1)/PI3K/Akt pathway induced by Insulin like Growth Factor 1 (IGF1), through the dephosphorylation of IRS-1, and that PTPL1 is sufficient to inhibit the IGF1 effect on cell survival and to induce apoptosis. Moreover, we have clarified the mechanisms by which the anti-estrogen OH-Tam inhibits Nerve Growth Factor (NGF)-induced breast cancer cells growth through PTPL1. PTPL1 is able to induce dephosphorylation of the NGF receptor, p140TrkA, which is necessary for activation of the MAPK (Mitogen Activated Protein Kinase) pathway and regulation of cell growth. Finally, in agreement with our retrospective studies showing the prognostic value of PTPL1 expression in ovarian tumors, we have also showed an inhibitory effect of PTPL1 on invasion, mobility and proliferation of ovarian cancer cells. Altogether, these results are consistent with the anti-oncogenic role played by this PTP. The identification of PTPL1 molecular targets and our understanding of the mechanisms that regulate its expression, its location and its activities, should allow consideration of new therapeutic approaches to regulate the expression or activity of this anti-oncogene
Tchankouo, Nguetcheu Stéphane. "Rôle des kinases LAMMER et des phosphatases PTP1B/PTP61F dans la régulation des voies de signalisation médiées par l'insuline." Thesis, Paris 11, 2012. http://www.theses.fr/2012PA11T067/document.
Повний текст джерелаType 2 diabetes and cancer represent the major public health problems. One important therapeutic target for these pathologies is the protein tyrosin phosphatase PTP1B. The phosphatase is known to negatively regulates the insulin signaling pathway by dephosphorylating the insulin receptor, IR or the insulin receptor substrate, IRS. However,PTP1B functions and its regulation mechanism remain poorly known. Two studies has notably described opposite effects of PTP1B activity following phosphorylation of its Ser50 residue either by CLK1/CLK2, LAMMER kinases or by AKT. Furthermore, AKT, a main insulin signaling pathway component, has been shown to phosphorylate the LAMMER kinaseCLK1 following insulin stimulation. In addition, the role of PTP1B in the regulation of the RAS/MAPK signaling pathway and hence in cancer is a very controversial subject. The first objective of this work was to analyse, the role of Ptp61F (the Drosophila ortholog of human PTP1B) in the Drosophila insulin pathway, the interaction between the phosphatase and the Drosophila LAMMER kinase gene, Doa, the role of Ptp61F in the RAS/MAPK signaling pathway. To achieve these, we took advantage of the genetic powerful of Drosophila to generate a Ptp61F gene mutant which has been characterized and its role in signaling pathways has been studied. This study showed that Ptp61F interacts with IR like PTP1B in mammals. It shows that Ptp61F regulates key components of insulin signaling pathway Pi3K/Akt. It also shows that Ptp61F is able to regulate the Drosophila LAMMER kinase gene, Doa. Finally, we noted that Ptp61F interacts by inhibiting the activity of severalcomponent of the RAS/MAPK signaling pathway of Drosophila (Egfr, Ras, rl (human ERK)) and conclude that Rl coud be a direct substrate of PTP61F. The data showing that Ptp61F interacts with Akt and the Drosophila LAMMER kinase gene, Doa, were the basis for the second study in order to show the role that the mammal LAMMER kinase CLK2 (Cdc-like kinase 2) could play in the insulin signaling pathway at molecular level using the human neuroblastoma cell line SH-SY5Y. From this second study, we show that CLK2 play an important role in insulin signaling. CLK2 is induced by insulin and its expression increases with time. PTP1B interacts in vivo and in vitro with CLK2. Overexpression of CLK2 impairs AKT phosphorylation by a mechanism which could involved PTP1B, since in vitro, CLK2 phosphorylates PTP1B and the latter interacts withAKT in vivo. It is the Ser50 residue of PTP1B being phosphorylated by CLK2 and this phosphorylation event represses PTP1B activity in vitro. AKT cannot phosphorylates PTP1B in vitro, suggesting that the phosphorylation of PTP1B by AKT could be cellular environment dependant
Cheyssac, Claire. "Etude de deux gènes candidats du DT2 : EIF4A2 : candidat positionnel au locus 3Q27 et PTPN1/PTP1B : cible pharmacologique dans la sensibilité à l'insuline." Lille 2, 2006. http://www.theses.fr/2006LIL2S009.
Повний текст джерелаType 2 diabetes (T2D) is the most common form of diabetes affecting more than 170 million people worldwide. The T2D pathophysiological mechanisms are characterized by defects of insulin secretion and insulin action leading to chronic hyperglycaemia determined by interactions between genetic and environmental risk factors. Although many genes responsible for monogenic forms of diabetes were identified, genetic determinants influencing T2D predisposition are still largely unknown. To identify new susceptibility variants, we used two approaches : - a familial association study of positional candidate gene variants at the 3q27 locus in falilies showing linkage to T2D with onset before 45 years ; and the exploration of a physiological candidate gene, PTPN1, through case-control analyses in different groups of subjects with type 2 diabetes or obesity. The analysis of the 3q27 locus in French families with strong T2D aggregation (432 diabetes subjects and 129 normoglycaemic subjects) confirmed of a genetic linkage with T2D age-of-onset. Two genes were investigated : KNG1, coding for kininogen, the bradykinin precursor, and EIF4A2 coding for the Eukaryotic Translation Initiation Factor 4 alpha 2, a translation initiation factor involved in protein synthesis which is down-regulated by glucose in rat pancreatic beta cells (INS832/13). A variant (rs266714), located upstream of the EIF4A2 gene showed association with T2D and T2D age-of-onset in the families. Affected sib-pairs sharing at least one at risk T allele showed a LOD-score of 5. 24 which could explain the T2D linkage. Moreover, this variant partly explains the age-of-onset linkage. The rs266714 SNP could modify the expression level of the eIF4A2 factor which modulates mRNA translation and protein synthesis rates in pancreatic beta cells. The PTPN1 gene codes for the protein tyrosine phosphatase 1B, a negative regulator of the insulin and leptin signalling pathways. An association with T2D and moderate obesity is observed for a variant at the PTPN1 gene locus. In 736 normoglycaemic non obese subjects, 2 intronic SNPs associate with variations of quantitative traits of glucose and lipid metabolism : increased HOMA-B and triglycerides, decreased HDL-cholesterol, which suggests a possible role in metabolic syndrome. This genetics approach contributes to an improved understanding of the pathways involved in the development of T2D and to propose new therapeutic targets
Sanchez-Blanco, Cristina. "Studies of the protein tyrosine phosphatase PTPN22/Lyp in Ptpn22 deficient mice." Thesis, King's College London (University of London), 2015. http://kclpure.kcl.ac.uk/portal/en/theses/studies-of-the-protein-tyrosine-phosphatase-ptpn22lyp-in-ptpn22-deficient-mice(cbcf6c1c-7d57-4df9-95ef-8696a7856858).html.
Повний текст джерелаMaisonneuve, Pierre. "Etude structurale et fonctionnelle de la phosphatase humaine PTPN4." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066673/document.
Повний текст джерелаThe function of signaling proteins is determined by the nature of the domains from which they are made up. A better understanding of cell signaling pathways will result from the study of these domains and their regulation. PTPN4 is a non-receptor tyrosine phosphatase with an anti-apoptotic function. Upon infection with an attenuated rabies virus, its function is hijacked, which subsequently leads to cell death. This phenotype is arises from the interaction of the PDZ binding motif (PBM) of the viral glycoprotein with the PDZ domain of PTPN4. In this study, we show that this PDZ domain is an allosteric inhibitor of the catalytic activity of the PTPN4 phosphatase domain. This is the first description of the regulation of a phosphatase by a PDZ domain. This inhibition is released by the interaction of a ligand to the PDZ domain, such as the viral glycoprotein PBM. Our structural study revealed that the PBM recognition disrupts the transient inter-domain interactions and restores the complete phosphatase catalytic properties. As well, we identified a PTPN4 endogenous ligand, the MAP Kinase p38, which may participate in the regulation of the cellular homeostatic through its interaction with PTPN4. Thus, in addition to its phosphatase regulatory role, the PDZ domain also allows the recruitment of partners and the introduction of substrates to the PTPN4 phosphatase active site. This study contributes to our understanding of the role played by PDZ domains in cell signaling pathways
Bertola, Débora Romeo. "Estudo do gene PTPN11 nos pacientes afetados pela síndrome de Noonan." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-12042006-110700/.
Повний текст джерелаINTRODUCTION: Noonan syndrome is an autosomal dominant disorder comprising short stature, facial dysmorphisms (ocular hypertelorism, downslanting palpebral fissures, palpebral ptosis, high arched palate and dental malocclusion), short and/or webbed neck, heart defects, mainly valvar pulmonary stenosis, sternal deformity and cryptorchidism in males. The PTPN11 gene, localized in the long arm of chromosome 12 (12q24.1), is responsible for approximately 50% of the cases. OBJECTIVE: To detect the PTPN11 gene mutation rate in a cohort of clinically well-characterized patients with Noonan and Noonan-like syndromes and to study the genotype-phenotype correlation. METHODS: Fifty probands with Noonan syndrome ascertained according to well-established diagnostic criteria, 3 with LEOPARD syndrome, 3 with Noonan-like/multiple giant cell lesion syndrome and 2 with neurofibromatosis/Noonan were enrolled in this study. Mutational analysis was performed using denaturing high-performance liquid chromatography followed by sequencing of amplicons with an aberrant elution profile. RESULTS: Missense mutations in the PTPN11 gene were identified in 21 probands with Noonan syndrome (42%), in all three patients with LEOPARD syndrome, in one case with Noonan-like/multiple giant cell lesion syndrome and in one with neurofibromatosis-Noonan syndrome. This last patient also showed a NF1 gene mutation. The only anomaly that reached statistical significance when comparing probands with and without mutations was the hematological abnormalities. A Noonan syndrome patient presenting a myeloproliferative disorder showed a T73I mutation. CONCLUSION: Noonan syndrome is a heterogeneous disorder, once PTPN11 gene mutations is responsible for 42% of the cases. A definitive genotype-phenotype correlation is not established, but the T73I mutation seems to predispose to a myeloproliferative disorder. Regarding Noonan-like syndromes, the PTPN11 gene is the main one in LEOPARD syndrome and also plays a role in neurofibromatosis-Noonan syndrome. Noonan-like/multiple giant cell lesion syndrome, part of the spectrum of Noonan syndrome, is also heterogeneous.
Keren, Boris Verloes Alain. "Syndrome de Noonan et mutations du gène PTPN11 corrélations génotype-phénotype /." Créteil : Université de Paris-Val-de-Marne, 2006. http://doxa.scd.univ-paris12.fr:80/theses/th0247734.pdf.
Повний текст джерелаChadwick, Michelle. "Characterization of a Novel Mouse Model for Angiosarcoma in Which Combined Inhibition of mTOR and MEK Results in Tumor Suppression." University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1490353768809798.
Повний текст джерелаAbdessamad, Mahmoud. "Caractérisation moléculaire et fonctionnelle des signalosomes PTEN/MAGI-1b/TRIP6 et PTEN/PTPN13." Paris 7, 2012. http://www.theses.fr/2012PA077048.
Повний текст джерелаThe PTEN tumor suppressor is a multifunctional protein endowed with a phosphatase activity that dephosphorylates not only some phosphotyrosine residues, but also the phosphoinositides generated by PI3K. In its C-terminus, PTEN encodes a PDZ-binding motif. Our Team has demonstrated the critical role of the lipid phosphatase activity of PTEN in stabilizing junctional complexes and in reverting invasiveness. PTEN interacts with cadherin β3-catenin complexes through the PDZ domain containing- protein MAGI-1. To identify new molecular PTEN partners, we applied yeast-two-hybrid assay, and identified TRIP6 and PTPN13. We have demonstrated that TRIP6 induces invasiveness of the MDCK epithelial cells, through i) the competition with (3-catenin for binding to MAGI-1 b and the destabilization of junctional complexes, ii) the activation of the PI3K/ Akt, and NFKB signaling pathways (FASEB J 23:916,2009). PTPN13 is a tyrosine phosphatase with 5 PDZ domains. We have confirmed the interaction of PTEN C-terminus with the 2nd PDZ domain of PTPN13 in vitro and ex vivo. We showed that PTEN is a PTPN13 substrate in vitro. In line with these results, the overexpression of wild-type PTPN13, but not of a mutant deficient in phosphatase activity decreased PTEN phosphorylation. This overexpression did not alter Akt phosphorylation -a reflect of the activity of this kinase- in PTEN deficient cell lines, but potentiated PTEN effects on this downstream target of PI3K. Thus, our results demonstrated the critical role of PTPN13 in controling PTEN signaling pathway
Xu, Dan. "EFFECTS OF ACTIVATING MUTATIONS IN SHP-2 (PTPN11) PHOSPHATASE ON HEMATOPOIETIC CELL DEVELOPMENT." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1295052361.
Повний текст джерелаPratigya, Gautam. "Deciphering the link between PTPN22 and autoimmunity." Thesis, Cardiff University, 2011. http://orca.cf.ac.uk/54471/.
Повний текст джерелаJariwala, Nidhi. "SND1 mediated downregulation of PTPN23 in HCC." VCU Scholars Compass, 2014. https://scholarscompass.vcu.edu/etd/3648.
Повний текст джерелаPadovani, Carolina Rabello. "Perfil cognitivo de pessoas portadoras da síndrome de Noonan com mutação do gene PTPN11." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/47/47133/tde-21052012-161923/.
Повний текст джерелаNoonan syndrome is an autosomal dominant genetically heterogeneous disorder. Although relatively high prevalence, there are few information about the cognitive profile of people with the syndorme. Current literature describes moderately impaired social cognition in terms of emotion recognition and emotion affection, besides a variable level of intelligence. Because of rarely researches about psychological area in this syndrome and, based on recent studies, the present study looked for clarify the cognitive profile of people with Noonan syndrome with mutation in the PTPN11 gene, trying to contribute for the establishment of a phenotypic behavior. 19 persons with the syndrome were studied, both male and female, diagnosticaded clinical and laboratorilly. Psychological assessment was realized by using Wechsler intelligence Scales, Rey Complex Figure Test and Wisconsin Card Sorting Test. The results indicated a variation of normal intelligence to mild mental retardation, besides inflexibility and not adapted responses using environmental feedback. The assessment checked the presence of lacked in categories achieved and, even, fault in planning of motor act (praxis) as responsible for low scores in graphic visuoconstruction episodic memory. These results suggest the necessity of expansion of studies which correlated cognitive aspects in the most variables genetic pathologics
Fodil, Mostefa. "Etude d'association génétique et analyse de gènes candidats différentiellement exprimés au cours de la polyarthrite rhumatoïde." Thesis, Evry-Val d'Essonne, 2015. http://www.theses.fr/2015EVRY0017/document.
Повний текст джерелаWe are interested in genetic study of rheumatoid arthritis “ra”, an autoimmune and chronic inflammatory disease affecting mainly joints and involving their destruction.“ra” is a multi-factor pathology in which the hla region seems to be the main but not unique genetic factor predisposition.for the first part of our study, we are interested in search of association with “ra” in the algerian population for genes polymorphisms already confirmed in other populations. this approach has allowed us establishing an association with solid evidence for the two most important snps ptpn22rs2476601 and stat4rs7574865. further studies with expanded samples and the inclusion of new genes polymorphisms candidates could establish new evidence of genetic association with pr population in algeria.in a second time, we are interested to family association analysis of genetic polymorphism gene having differential expression in pr. this part of the study represents supplementary information for understanding genes these operating and their involvement in pr. this work analysis genetics association was supplemented by the study of relationship between genotypes of snps studied and the rate of gene expression considered.polymorphisms of interest shown during this study represent good targets for a combined analysis of “ra” in both french and algerian populations to establish an ethnic comparison of various factors for genetic predisposition to “ra”
Ackermann, Nadine [Verfasser], Kai Sven [Gutachter] Erdmann, and Rolf [Gutachter] Heumann. "Biochemische und funktionelle Untersuchung der Multidomänenproteine PTPN13 und OCRL1 / Nadine Ackermann ; Gutachter: Kai Sven Erdmann, Rolf Heumann." Bochum : Ruhr-Universität Bochum, 2013. http://d-nb.info/1129451682/34.
Повний текст джерелаFerreira, Lize Vargas. ""Estudo do gene PTPN11 em pacientes com a síndrome de Noonan e crianças com baixa estatura idiopática"." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-02092005-100524/.
Повний текст джерелаNoonan syndrome (NS), characterized by short stature, dysmorphic facial and thoracic features and congenital heart disease, was associated to PTPN11 gene. We studied the PTPN11 in patients with NS, parents of mutation-positive NS patients and idiopathic short stature children with signs related to NS without fulfilling the diagnostic criteria. We found missense mutations in 42.3% of the NS group. Parents of NS mutation-positive patients did not present mutations, nor did children with short stature. The only statistically significant difference between groups with and without mutations was response to long term use of hGH, better on the mutation-negative group
Lefevre, D., N. Cranley, and Ø. Holmeide. "PTPV1 and PTPV2 Translation in FTI Systems." International Foundation for Telemetering, 2013. http://hdl.handle.net/10150/579686.
Повний текст джерелаA Flight Test Instrumentation (FTI) system may consist of equipment that either supports PTPv1 (IEEE 1588 Std 2002) or PTPv2 (IEEE 1588 Std 2008). The challenge in such time distributed system is the poor compatibility between the two PTP protocol versions. This paper describes how to combine the PTP versions in the same network with minimum or no manual configuration.
Babault, Nicolas. "Etude structurale du domaine PDZ de PTPN4, une cible pour le déclenchement de la mort neuronale." Paris 6, 2011. http://www.theses.fr/2011PA066211.
Повний текст джерелаBrasil, Amanda Salem. "Estudo dos genes PTPN11 e KRAS em pacientes afetados pela síndrome de Noonan e pelas síndromes Noonan-like." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-25022010-140048/.
Повний текст джерелаINTRODUCTION: Noonan syndrome shows autosomal dominant inheritance, and is a relatively frequent disease in the population, with an estimated incidence between 1/1000 and 1/2500 live births. The main clinical features are: facial dysmorphisms, short stature, cryptorchidism and cardiac abnormalities. The differential diagnosis between Noonan syndrome and Noonan-like syndromes is not always easy, due to the overlap of the their clinicla findings. The Noonan-like syndromes, more rare that the Noonan syndrome, include the LEOPARD syndrome, neurofibromatosis-Noonan, cardiofaciocutaneous and Costello. Currently it is known that Noonan syndrome and Noonan-like syndromes involve mutations in genes belonging to the RAS-MAPK signaling pathway. In Noonan syndrome, at least four genes of this pathway are responsible for the phenotype: PTPN11, SOS1, RAF1 and KRAS. Mutations in PTPN11, the first gene described in association with this syndrome, are found in approximately 40% of cases. The second gene described, the KRAS gene, is responsible for about 2% of the cases that dont have mutations in the PTPN11 gene. Mutations in the KRAS gene are present in patients with Noonan syndrome with mental retardation and/or developmental delay more pronounced and in patients with cardiofaciocutaneous syndrome whose ectodermal involvement is more subtle. OBJECTIVE: Due to the recent association of the KRAS gene with Noonan and Noonan-like syndromes is important: (1) to test the frequency of mutation in this gene in patients with or without mutations in PTPN11 and (2) to estabilish a more precise genotype-phenotype correlation, allowing the realization of a more appropriate genetic counseling. METHODS: 95 probands with Noonan syndrome and 30 with Noonan-like syndromes were evaluated. The molecular analysis was performed by the polymerase chain reaction, followed by purification and bidirectional sequencing. RESULTS: PTPN11 gene mutation was found in 20/46 (43%) patients with Noonan syndrome, two of them not previously described. By correlating the clinical features of patients with Noonan syndrome in this study, with or without mutations in the PTPN11 gene, it was noted that patients with mutations have significantly higher incidence of short stature, pulmonary stenosis and lower incidence of hypertrophic cardiomyopathy. Mutations in KRAS gene were found in two patients a patient with Noonan syndrome ant the other with Costello syndrome. Gene alterations in more than one gene at the RASMAPK patway were observed in two patients, but one of the mutations in each patient didnt predict a significant phenotypic effect. Were also foud three polymorphisms in the KRAS gene, but with the same frequency in the control group. To check the influence of these polymorphisms, the main features of Noonan syndrome were related among patients with this syndrome who had mutations in the PTPN11 gene and compared of the presence or absence of these polymorphisms. No statistically significant difference was found. CONCLUSION: Patients with Noonan syndrome and PTPN11 gene mutation had a higher incidence of short stature and pulmonary valve stenosis and a lower incidence of hypertrophic cardiomyopathy. The KRAS gene, previously related to Noonan and cardiofaciocutaneous syndrome, was also responsible for Costello syndrome. Gene alterations considered as nonpathogenic and polymorphisms found in the KRAS gene seem to have a not great influence on the phenotypic variability in Noonan syndrome. However, it is not possible to completely rule out that these changes have a subtle effect and that, together with other genetic variations and/or environmental factors, may have a modulating effect
Zheng, Peilin [Verfasser], and Stephan [Akademischer Betreuer] Kissler. "Ptpn22 silencing in the NOD model of type 1 diabetes indicates the human susceptibility allele of PTPN22 is a gain-of-function variant / Peilin Zheng. Betreuer: Stephan Kissler." Würzburg : Universitätsbibliothek der Universität Würzburg, 2012. http://d-nb.info/1028327145/34.
Повний текст джерелаSood, Shatakshi. "Role of protein Tyrosine Phosphatase PTPN22 in T cell signalling and autoimmunity." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/10490.
Повний текст джерелаNaß, Alexandra [Verfasser]. "Investigations on Key Principles of PTP1B Selectivity / Alexandra Naß." Berlin : Freie Universität Berlin, 2018. http://d-nb.info/1176708279/34.
Повний текст джерелаRibeiro, Alexsandra Christianne Malaquias de Moura. "Avaliação do padrão de crescimento na síndrome de Noonan em pacientes com mutações identificadas nos genes PTPN11, SOS1, RAF1 e KRAS." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-17062011-160529/.
Повний текст джерелаNoonan Syndrome (NS) is characterized by distinctive facial features, short stature and congenital heart defects. The estimated prevalence is 1:1000 to 1:2500 live births, affecting equally both sexes. It is an autosomal dominant disorder with complete penetrance, but most cases are sporadic. To date, mutations in the RAS/MAPK pathway genes (PTPN11, KRAS, SOS1, RAF1, MEK1, NRAS and SHOC2) were identified in approximately 70% of patients. One of the cardinal signs of NS is proportional postnatal short stature although the physiopathological mechanism of growth impairment remains unclear. The current knowledge about the natural history of growth associated with NS was described before molecular diagnosis era. In this study, we performed PTPN11, SOS1, RAF1, and KRAS mutation analysis in a cohort of 152 NS patients and studied the natural linear (height) and ponderal growth [body mass index (BMI)] of NS patients with related mutations. Mutations in NS-causative genes were found in 99 patients (65%) of our cohort. The most common mutated gene was PTPN11 (47%), followed by SOS1 (9%), RAF1 (7%) and KRAS (3%). Sex-specific percentile curves for height and BMI were constructed using the LMS method. NS patients had birth weight and length within normal ranges but the postnatal growth impairment was observed during the first year of life, reaching a final height of -2.3 and -2.2 standard deviations from the mean for Brazilian healthy men and women, respectively. Postnatal growth impairment was higher in RAF1 mutation patients than in patients with SOS1 and PTPN11 mutations. BMI values in NS patients were lower in comparison with normal Brazilian population. BMI values were higher in patients with RAF1 mutations than in patients with other genotypes. Patients with mutations in PTPN11 and SOS1 genes were more likely to have pulmonary valve stenosis, whereas hypertrophic cardiomyopathy was more common in patients with mutations in the gene RAF1. The intensity of constitutive tyrosine phosphatase activity of SHP-2 due to PTPN11 mutations, as well as the presence of polymorphisms in KRAS gene did not influence the phenotype of NS patients with mutation in PTPN11 gene. Analysis of exons 3, 8 and 13 of PTPN11 gene, followed by exons 6 and 10 of SOS1 gene and exon 7of RAF1 gene identified 86% of patients harboring mutations in related genes, suggesting a more efficient evaluation of NS molecular diagnosis. We believe that the phenotypic variability in this syndrome is directly linked to the different roles played by proteins that participate in RAS/MAPK pathway. However, further studies in RAS/MAPK pathway are needed to clarify issues related to growth and other phenotypic characteristics of SN
Burn, Garth Lawrence. "PTPN22/Lyp : a novel regulator of integrin signalling and function in T lymphocytes." Thesis, King's College London (University of London), 2015. https://kclpure.kcl.ac.uk/portal/en/theses/ptpn22lyp-a-novel-regulator-of-integrin-signalling-and-function-in-t-lymphocytes(126c4e96-15a5-4c11-89b1-f21a6310098e).html.
Повний текст джерелаJohnson, Kevin. "PTP1B regulation of the transcription factor Stat5 in breast cancer." Connect to Electronic Thesis (CONTENTdm), 2009. http://worldcat.org/oclc/454241197/viewonline.
Повний текст джерелаBray, Cara. "Using CRISPR to determine the effects of mutations of PTPN22 in human T cells." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31418.
Повний текст джерелаDelile, Eugénie. "Impact de la délétion totale et endothéliale de PTP1B sur la dysfonction cardiovasculaire et l'insulino-résistance dans un modèle de sepsis sévère expérimental." Thesis, Normandie, 2017. http://www.theses.fr/2017NORMR011/document.
Повний текст джерелаHyperglycemia and insulin resistance are septic metabolic alterations associated with poorprognosis, increased cardiovascular dysfunction and mortality. Several studies have demonstrated thathigh-dose insulin therapy reduces mortality and prevents multi-organ dysfunction but is controversialbecause it is often associated with deleterious hypoglycemia. Protein Tyrosine Phosphatase 1B (PTP1B)is a negative regulator of both insulin signaling and NO production.The concept developed in our laboratory is that PTP1B inhibition could be a potentialtherapeutic target in sepsis by improving both insulin sensitivity and these consequences onendothelial and cardiac function. PTP1B genetic deletion has been shown to decrease cardiovasculardysfunction in sepsis but the effects of this deletion on carbohydrate metabolism in the improvementof cardiovascular dysfunction remain unknown and constitute the objective of our work.In a sepsis model induced by Ligature and Caecal Perforation, we have demonstrated that thetotal PTP1B genetic deletion limits insulin resistance induced by sepsis, improves the AMPK signalingpathway, the GLUT-4 translocation and reduces inflammation. These effects are followed by decreasedendothelial dysfunction induced by sepsis and improves NO production. The endothelial PTP1B geneticdeletion, significantly improves endothelial function, insulin and glucose sensitivity.This work demonstrate the beneficial effect of the PTP1B genetic deletion in the sepsis byimprovement of the insulin sensibility and these consequences on endothelial and cardiac function
Kokkonen, Heidi. "Pathogenetic factors of importance for the development and progression of rheumatoid arthritis." Doctoral thesis, Umeå universitet, Institutionen för folkhälsa och klinisk medicin, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-54003.
Повний текст джерелаHébert, Chatelain Etienne. "Impact des phosphorylations sur tyrosine sur le métabolisme mitochondrial : régulation et impacts fonctionnels des phosphorylations induites par la Src kinase." Thesis, Bordeaux 2, 2011. http://www.theses.fr/2011BOR21830/document.
Повний текст джерелаMitochondria are implicated in several key cellular processes. They are producing most part of the energy that is consumed by the cell via oxidative phosphorylation processes (OXPHOS). Phosphorylation of different components implicated in OXPHOS are known to constitute an important regulation pathway of energetic production. The objective of this thesis was to understand how tyrosine phosphorylation induced by the Src kinase could influence OXPHOS. First, it was shown that Src kinase mediated phosphorylation can be regulated directly in mitochondria, inducing phosphorylation of several mitochondrial proteins and different effects on OXPHOS. I also demonstrated that Src kinase is also present in mitochondria of cancer cells where it can lead to phosphorylation of NADH-oxidoreductase. This phosphorylation site is associated with increase of OXPHOS which could be implicated in the establishment of global phenotype of cancer cells
Couture, Pascal. "Role of a PTP1B Pathway in the Neuropsychiatric Expression of a Mouse Maternal Immune Activation Model." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/38891.
Повний текст джерелаWagner, Johanna. "Die Rolle von IL12B- und PTPN2-Polymorphismen bei der Pathogenese chronisch entzündlicher Darmerkrankungen." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-174373.
Повний текст джерелаCastro, Dopico Xaquin. "A PTPN22 gene-to-phenotype study ; and, The evaluation of IL-2 immunotherapy for type 1 diabetes." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.707951.
Повний текст джерелаChiarreotto-Ropelle, Eloize Cristina 1978. "Caracterização da atividade da PTP1B em hipotálamo de roedores obesos submetidos ao exercício físico." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/244471.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Aplicadas
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Resumo: A ingestão alimentar e o gasto energético são minuciosamente regulados por neurônios específicos localizados no hipotálamo. O funcionamento adequado desta complexa rede neuronal é determinante para a manutenção da homeostase energética em mamíferos. No entanto, a inflamação hipotalâmica está associada com a resistência à insulina e leptina, obesidade e hiperfagia. Neste contexto, proteína tirosina fosfatase 1B (PTP1B) hipotalâmica surgiu como a fosfatase chave responsável pela resistência central à insulina e à leptina. O objetivo do atual estudo foi avaliar o efeito do exercício físico agudo sobre a expressão da PTP1P hipotalâmica em roedores obesos. Nossos resultados demonstraram que o exercício físico reduziu a inflamação e os níveis proteicos e a atividade da PTP1B no hipotálamo de animais obesos. O exercício físico reduziu a interação entre a PTP1B com proteínas envolvidas na via de transmissão do sinal da insulina (IRbeta e IRS1) e da leptina (Jak2), melhorando os sinais anorexigênicos mediados por esses hormônios. De forma interessante, o efeito anti-inflamatório e o efeito inibitório sobre a PTP1B mediados pelo exercício ocorreu de forma dependente da Interleucina-6 (IL-6), uma vez que o exercício não reduziu a inflamação e os níveis proteicos PTP1B após a inibição específica da IL-6 hipotalâmica em animais obesos. Por outro lado, a administração intracerebroventricular (ICV) do IL-6 recombinante reproduziu os efeitos do exercício, melhorando a ação da insulina e leptina hipotálamo, reduzindo a sinalização inflamatória e atividade PTP1B em ratos obesos em repouso. Coletivamente, o nossos resultados demonstraram que o exercício físico restaurou a sinalização de insulina e da leptina no hipotálamo de animais obesos, pelo menos em parte, pela redução da expressão e atividade da PTP1B hipotalâmica, sendo esse efeito decorrente da resposta anti-inflamatória mediada, e também dependente, de IL-6
Abstract: The food intake and energy expenditure are regulated by specific neurons in the hypothalamus. The proper functioning of this complex neuronal network is crucial to the maintenance of energy homeostasis in mammals. However, hypothalamic inflammation is associated with insulin and leptin resistance, hyperphagia and obesity. In this scenario, hypothalamic protein tyrosine phosphatase 1B (PTP1B) has emerged as the key phosphatase induced by inflammation responsible for the central insulin and leptin resistance. The aim of this study was evaluate the effects of exercise on PTP1B activity in the hypothalamus of obese rodents. Here, we demonstrated that acute exercise reduced inflammation and PTP1B protein level/activity in the hypothalamus of obese rodents. Exercise disrupted the interaction between PTP1B with proteins involved in the early steps of insulin (IRbeta and IRS1) and leptin (Jak2) signaling, increased the anorexigenic effects of insulin and leptin in obese rats. Interestingly, the anti-inflammatory action and the reduction of PTP1B activity mediated by exercise occurred in an Interleukin-6 (IL-6)-dependent manner, because exercise failed to reduce inflammation and PTP1B protein level after the disruption of hypothalamic-specific IL-6 action in obese rats. Conversely, intracerebroventricular (ICV) administration of recombinant IL-6 reproduced the effects of exercise, improving hypothalamic insulin and leptin action by reducing the inflammatory signaling and PTP1B activity in obese rats at rest. Taken together, our study reports that physical exercise restores insulin and leptin signaling, at least in part, by reducing hypothalamic PTP1B protein level through the central anti-inflammatory response mediate by IL-6
Mestrado
Metabolismo e Biologia Molecular
Mestra em Ciências da Nutrição e do Esporte e Metabolismo
Veenstra, Cynthia. "The receptor tyrosine kinase Met and the protein tyrosine phosphatase PTPN2 in breast cancer." Doctoral thesis, Linköpings universitet, Avdelningen för kliniska vetenskaper, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-135047.
Повний текст джерелаLopes, Antonio Luiz Ribeiro Boechat. "Influencia de polimorfismos dos genes TNF e PTPN22 sobre a artrite reumatoide e a tuberculose no Amazonas, Brasil." Universidade Federal do Amazonas, 2010. http://tede.ufam.edu.br/handle/tede/4302.
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Rheumatoid Arthritis (RA) is a autoimmune disease that affects 1% of worldwide population and promotes polyarthritis and joint destruction with work disability. About 60% of the risk factors of RA are related to genetic characteristics of individuals. The functional polymorphisms of the TNF gene -308 G/A andPTPN22 1858 C/T were associated with RA in several studies.The aim of this study was to investigate the influence of the TNF 308 G/A polymorphism in the promoter region of the tumor necrosis factor- gene and PTPN22 1858 C/T on rheumatoid arthritisand tuberculosis patients from the Brazilian Amazon. A total of 545 individuals 205 healthy controls without arthritis and 132 individuals suffering from rheumatoid arthritis and 208 tuberculosis patients were genotyped for these polymorphisms using a methodology based on PCR-RFLP. Rheumatoid factor, age more than 60 years old and more than 10 disease years, was found to be risk factors for systemic disease (p=0,0001). The frequency of the A allele (TNF2) in rheumatoid arthritis sufferers was not found to be significantly higher than in the controls (p=0.671; OR=1.16; confidence interval=0.59 – 2.25). However, using a logistic regression model when the patients were stratified according to whether the manifestations were preponderantly articular or systemic, there was a strong association between the TNF2 allele and the systemic disease (p=0.001; OR=4.75; confidence interval=1.82 – 12.40) as well as the use of anti-TNF immunotherapy (p=0.021; OR 2.93; confidence interval=1.15 –7.46). On the other hand, PTPN22 1858T allele was not associated with systemicdisease (p=0,071; OR=3,17; confidence interval=0.83 – 11.73), but we found association between this allele and biologic anti-TNF immunotherapy (p=0,021; OR=4.39; confidence interval=1.08 – 17.86). Moreover, there was found no association between PTPN22 15858 C/T and rheumatoid arthritis nor tuberculosis. These results suggest that the TNF2 allele is associated with the more serious forms of the disease in individuals from the Brazilian Amazon but not with a risk for developing RA.
A Artrite Reumatoide (AR) é uma doença autoimune que afeta 1% da população geral, promovendo poliartrite e destruição articular com variados graus de incapacidade. Cerca de 60% dos fatores de risco da AR são relacionados a caracteres genéticos do indivíduo. Os polimorfismos funcionais dos genes TNF -308 G/A e PTPN22 1858 C/T foram associados à AR e à Tuberculose em diversos estudos. O objetivo deste estudo foi analisar a influência do polimorfismo da região promotora do gene do Fator de Necrose Tumoral-α, TNF -308 G/A e do gene PTPN22 1858 C/T na Artrite Reumatoide (AR) e na Tuberculose pulmonar em indivíduos procedentes do Amazonas. Para isso, foram genotipados, pela técnica baseada em PCR-RFLP para o polimorfismo TNF 308 G/A (TNF2) e PTPN22 1858T, 545 indivíduos sendo 205 controles sem Artrite, 208 pacientes com Tuberculose e 132 portadores de AR. Não foi observado aumento da frequência dos alelosTNF2ouPTPN22 1858T em portadores de Artrite Reumatoide e Tuberculose em comparação aos controles (p=0,218; p=0,376, respectivamente). Foram identificados como fatores preditivos para manifestações sistêmicas da Artrite Reumatoide: o Fator Reumatoide positivo, a idade maior que 60 anos e o tempo de doença (p=0001).Quando os dados foram estratificados segundo as formas predominantemente articulares ou sistêmicas, o alelo TNF2 esteve fortemente associado às formas sistêmicas (p=0,001; OR=4,75; Intervalo de confiança = 1,82 – 12,40), além de estar associado ao uso de imunobiológicos Anti-TNF (p=0,021; OR=2,93; Intervalo de confiança=1,15 – 7,49). O alelo PTPN22 1858T também está associado ao uso de imunobiológicos (p=0,021; OR= 4,39; Intervalo de Confiança=1,08 – 17,86), mas não está relacionado às formas sistêmicas (p=0,071; OR = 3,17; Intervalo de Confiança = 0,83 – 11,73). Entretanto, o polimorfismo de PTPN22 1858 C/T não foi associado à Artrite Reumatoide ou à Tuberculose Pulmonar. Estes resultados sugerem que o alelo TNF2 está associado às formas mais graves da AR em indivíduos do Amazonas, mas não ao risco de desenvolver Artrite Reumatoide.
Santaniemi, M. (Merja). "Genetic and epidemiological studies on the role of adiponectin and PTP1B in the metabolic syndrome." Doctoral thesis, University of Oulu, 2010. http://urn.fi/urn:isbn:9789514261855.
Повний текст джерелаTiivistelmä Metabolinen oireyhtymä on kertymä tekijöitä, jotka altistavat tyypin 2 diabetekselle ja sydän- ja verisuonitaudeille. Keskivartalolihavuus ja insuliiniresistenssi, eli insuliinin heikentynyt teho, vaikuttavat olevan keskeisiä metabolisessa oireyhtymässä. Kuitenkaan taustalla olevaa syntymekanismia ei täysin tunneta. Väitöskirjatyön tavoitteena oli tutkia PTP1B- ja adiponektiinigeenin muuntelun sekä plasman adiponektiinitason yhteyttä metaboliseen oireyhtymään, sen osatekijöihin ja seurauksiin. PTP1B on insuliinin toimintaa soluissa estävä molekyyli. Ensimmäisessä tutkimuksessa havaittiin että kolme tutkittua PTP1B-geenin nukleotidimuutosta eivät ole vahvasti yhteydessä tyypin 2 diabetekseen. Eräs nukleotidimuutos saattaisi olla lievästi suojaava tyypin 2 diabetesta vastaan, sillä se oli yleisempi terveillä kuin tyypin 2 diabetesta sairastavilla. PTP1B:n ja leptiinireseptorigeenin eräiden alleelien yhdistelmä oli yhteydessä painoindeksiin. Adiponektiini on rasvakudoksen erittämä hormoni, jolla on suotuisia, insuliinin vaikutusta edesauttavia vaikutuksia elimistössä sekä edullisia vaikutuksia verenkiertoelimistössä. Toisessa työssä havaittiin että Amerikan valkoihoisilla, joilla oli eräs harvinainen adiponektiinigeenin alleeli (Tyr111His), oli heikompi insuliinin teho kuin henkilöillä joilla ei ollut kyseistä muutosta. Tämä alleeli oli yleisempi suomalaisilla tyypin 2 diabetesta sairastavilla kuin terveillä, mikä saattaa tarkoittaa että se liittyy suurentuneeseen riskiin tyypin 2 diabetekselle. Afroamerikkalaisilla taas toiset nukleotidimuutokset olivat yhteydessä lihavuuteen ja plasman rasva-arvoihin. Adiponektiinin pitoisuutta plasmassa mitattiin erilaisissa aineistoissa. Kolmannessa tutkimuksessa havaittiin, että matala pitoisuus oli yhteydessä metabolisen oireyhtymän eri osatekijöihin ja pitoisuus oli sitä matalampi, mitä enemmän osatekijöitä henkilöllä on. Neljännessä tutkimuksessa havaittiin että matala plasman adiponektiinipitoisuus oli yhteydessä suurentuneeseen riskiin saada huonontunut glukoosin sietokyky tai tyypin 2 diabetes tulevaisuudessa. Viidennessä tutkimuksessa adiponektiinitaso määritettiin naisilta jotka olivat ohittaneet vaihdevuodet ja saivat estrogeenikorvaushoitoa. Havaittiin että plasman adiponektiinitaso laski niillä naisilla, jotka saivat korvaushoitoa suun kautta. Tämä saattaisi osittain selittää suun kautta annettavan estrogeenikorvaushoidon epäedullista vaikutusta sydän ja -verisuonitautien riskitekijöihin. Tutkimus vahvistaa edelleen adiponektiinin merkitystä lihavuuteen liittyvissä sairauksissa ja tuo uutta tietoa adiponektiini- ja PTP1B-geenien muuntelun merkityksestä eri väestöissä
Genera, Mariano. "Structural and functional study of the human phosphatase PTPN3 and its interaction with oncogenic viruses." Electronic Thesis or Diss., Sorbonne université, 2019. http://www.theses.fr/2019SORUS112.
Повний текст джерелаThe human protein tyrosine phosphatase non-receptor type 3 (PTPN3) is a PDZ (PSD-95/Dlg/ZO-1) domain-containing phosphatase with a tumor-suppressive or a tumor-promoting role in many cancers, although its role in cell signalling is still unclear. Interestingly, the high-risk genital human papillomavirus (HPV) types 16 and 18 and the hepatitis B virus (HBV) target the PDZ domain of PTPN3 through PDZ-binding motifs (PBMs) in their E6 and HBc proteins. Here, I report a detailed study of the interactions between the PDZ domain of PTPN3 and its cellular and viral ligands. First, we combined biophysical, NMR and X-ray experiments to investigate the structural and functional properties of the PDZ domain of PTPN3 and its interaction with the E6 PBM. We then extended our structural study of PTPN3-PDZ to other cellular and viral partners, and gained insights into the main structural determinants of recognition of PBMs. We then focused on the HBV HBc protein. We screened a library of human PDZ-containing proteins for HBc binders and identified 28 cellular HBc-interacting partners, most of which are involved in cell polarity. We confirmed that PTPN3 can bind the HBc PBM in the context of the viral capsid, and we showed that viral PBMs interact with PTPN3-PDZ with similar affinities to endogenous PTPN3 ligands. Using HBV-infected hepatocytes we observed that overexpression of PTPN3 has multiple effects on HBV infection. Finally, we investigated the interactome of PTPN3-PDZ to gain insights into the role of this protein in cell signalling and the disruptive effects of HBV
Moise, Gwendolyn. "Investigations into Factors Affecting the WPD-Loop in the Protein Tyrosine Phosphatases YopH and PTP1B." DigitalCommons@USU, 2018. https://digitalcommons.usu.edu/etd/7226.
Повний текст джерелаKuga, Gabriel Keine [UNESP]. "Efeitos biomoleculares do exercício físico sobre a disfunção na via de sinalização da insulina em hipocampo de ratos envelhecidos." Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/155987.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A insulina e o fator neurotrófico derivado do cérebro (BDNF) no hipocampo promovem a plasticidade sináptica e a formação da memória no hipocampo. Por outro lado, o envelhecimento está relacionado ao declínio cognitivo e é o principal fator de risco para a doença de Alzheimer (DA). No entanto, a comunidade científica tem feito grande esforço para elucidar os mecanismos moleculares responsáveis pela patogênese da DA que são disparados pelo envelhecimento. A Proteina Tirosina Fosfatase 1B (PTP1B) está relacionada a vários processos deletérios nos neurônios e pode ser alvo promissor para novas terapias, e também regulada pelo exercício físico. Neste contexto, nosso estudo teve como objetivo investigar as alterações relacionadas com o envelhecimento sobre o conteúdo de PTP1B, sinalização da insulina e conteúdo de β-amilóide no hipocampo de ratos de meia-idade, bem como o possível efeito terapêutico do exercício físico. Ratos Wistar jovens (3 meses de idade) e de meia-idade (sedentários e exercitados) (17 meses de idade) foram submetidos ao teste do Labirinto Aquático de Morris (MWM), ao teste de tolerância à glicose e à análise molecular do tecido hipocampal através da técnica de Western Blot. Os dados foram analisados através da Análise de Variância (ANOVA) one-way com nível de significância estabelecido abaixo de 0,05. Os ratos realizaram protocolo de exercício físico de natação durante 5 dias consecutivos, com 2 horas de duração por dia. Através dos resultados, verificou-se que o envelhecimento resultou em aumento do peso corporal e intolerância à glicose, bem como diminuiu o processo de aprendizagem no MWM. Observou-se também que os ratos de meia-idade têm níveis mais altos de PTP1B, e isso está relacionado a menor fosforilação de Substrato do Receptor de Insulina-1 (IRS-1), Proteína Kinase B (Akt), Glicogênio Sintase Kinase β (GSK3β), e Receptor Tirosina Kinase Beta (TrkB). Além disso, o processo de envelhecimento aumentou o conteúdo β-amilóide no hipocampo. Por outro lado, o exercício físico foi eficiente em melhorar a tolerância à glicose e o desempenho no MWM, bem como em restaurar a fosforilação da Akt e reduzir o conteúdo de β-amilóide. Em conclusão, este estudo fornece novas evidências de que o conteúdo de PTP1B no hipocampo está aumentada com o envelhecimento e isto está relacionado com alterações cognitivas, e, por outro lado, o exercício físico atenua esse processo em ratos envelhecidos.
The insulin and Brain-Derived Neurotrophic Factor (BDNF) signaling in the hippocampus promote synaptic plasticity and memory formation. On the other hand, aging is related to the cognitive decline and is the main risk factor for Alzheimer’s Disease (DA). Nevertheless, a great effort has been made by the scientific community to elucidate the molecular mechanism of aging-related DA pathogenesis. The Protein-Tyrosine Phosphatase 1B (PTP1B) is related to several deleterious processes in neurons and emerges as a promising target for new therapies, like as physical exercise. In this context, our study aims to investigate the age-related changes in hippocampal PTP1B content, insulin signaling, β-amyloid content in the hippocampus of middle-aged rats, and the possible therapeutic effect of exercise. Young (3 months-old) and Middle-aged (17 months-old) Wistar rats were submitted to Morris-water maze (MWM) test, glucose tolerance test, and to molecular analysis in the hippocampus. The rats performed a 2-hour swim physical exercise protocol for 5 consecutive days. Aging resulted in increased body weight, and glucose intolerance and decreases learning process in MWM. Interestingly, the Middle-Aged rats have higher levels of PTPB, and this is related to lower phosphorylation of Insulin Receptor Substrate-1 (IRS-1), Protein Kinase B (Akt), Glycogen Syntase Kinase β (GSK3β), and Tyrosine Kinase Receptor Beta (TrkB). Also, the aging process increased β-amyloid content in the hippocampus. On the other hand, the physical exercise was efficient to improve glucose tolerance and MWM performance, as well as to restore Akt phosphorylation and reduce β-amyloid content. In conclusion, this study provides new evidence that PTP1B content in the hippocampus is increased with aging and this is related to cognitive alterations, and physical exercise can attenuate this process in middle-aged rats.
Michou, Laëtitia. "Approches génétiques de la polyarthrite rhumatoïde." Paris 5, 2007. http://www.theses.fr/2007PA05P603.
Повний текст джерелаThe aim of this work was to search for rheumatoid arthritis (RA) genetic factors using different clinical and molecular genetic approaches. The clinical genetic approach of this work led to exhibit familial aggregation of RA and autoimmune diseases (AID), and to show that some characteristics of the index case (RA age of onset and sex) were associated to an increased risk of RA and/or AID in the relatives. There seemed to be an influence of personal and/or familial autoimmunity on the results of linkage analysis, which probably needs to be studied on larger samples. The modelisation by MASC method of the HLA component in RA has up to now rejected the model in which a unique shared epitope (SE) should explain the disease susceptibility. A new classification based on the presence or not of the RAA motif at position 72 to 74, but modulated by the aminoacid at position 71 and the aminoacid at position 70 was proposed and allowed not to reject the SE hypothesis. This new classification was replicated in an independent sample. The candidate genes approach allowed to select 187 genes among the 1577 genes of known function in the 19 chromosomal regions suggested by the dense genome-wide scan. A linkage/association on a French and European sample of 465 trio families provided a linkage proof between the PTPN22-1858T polymorphism and RA, in the addition of the association reported in numerous publications in the Caucasian population. However, the association study of BlyS and CRLR genes, both located in suggested chromosomal regions by linkage analysis and good candidate genes by their function, were not associated with RA in a sample of 100 trio families. Finally, the search for gene-environment interaction in familial forms of RA led to show an interaction between tobacco, anti-CCP and the HLA-DRB1*0401, underlining a particular role of this allele among SE in this interaction
Bayley, Rachel. "Altered leukocyte signalling thresholds in rheumatoid arthritis through changes in the function of the protein tyrosine phosphatase PTPN22/LYP." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/5060/.
Повний текст джерелаHanna, Nadine. "Maladies héréditaires liées à une dérégulation de la voie Ras-MAPK : Conséquences des mutations de PTPN11 associées au syndrome LEOPARD et étude des corrélations génotype/phénotype dans les syndromes NCFC." Paris 5, 2008. http://www.theses.fr/2008PA05T023.
Повний текст джерелаNeuro-Cardio-Facio-Cutaneous (NCFC) syndromes groups four clinically related developmental disorders: Noonan (NS), LEOPARD (LS), Cardio-Facio-Cutaneous (CFC) and Costello (CS) syndromes. Discovery of PTPNll mutations, coding the phosphatase SHP-2, in NS was the key of the identification of mutations of numerous actors of the Ras-MAPK pathway in the NCFC syndromes. Whereas NS mutations enhance SHP-2 in vitro catalytic activity, we showed that activity of LS mutants is decreased and promote in cellulo Gabl/PI3K binding. How these apparently opposite behaviours of SHP-2 mutants lead to clinically overlapped syndromes still remain to be explained. On the other hand, we performed a genotype/phenotype correlation study in a large NCFC patients cohort. Each of the mutations affecting the Ras-MAPK pathway, by its functional consequences and by the level of the pathway affected, determines a particular symptomatology demonstrating a phenotypic continuum between the clinical entities
Goëb, Vincent. "Identification de marqueurs diagnostiques de la polyarthrite rhumatoïde par une double approche génétique et protéomique." Rouen, 2008. http://www.theses.fr/2008ROUES032.
Повний текст джерелаThe interest of TNFRII 196R, PTPN22 1858T and HLA-shared epitope alleles (HLA-SE) for rheumatoid arthritis (RA) diagnosis was assessed. TNFRII 196R and HLA-SE alleles are associated with RA diagnosis but their study, in a cohort of patients with very early arthritis (VerA cohort) does not improve those of antibodies against citrullinated proteins and/or rheumatoid factors which led to predict RA outcome in 68% of VerA cohort after 2 years of follow-up. In order to improve the sensitivity of the autoimmune diagnosis markers, we used proteomic tools (two-dimensional electrophoresis with protein extract from HL-60 and MALDI-TOF mass spectrometry), which allowed is to highlight several citrullinated enzymes of the glycolytic pathway and molecular chaperonins as new early autoentigens
Lin, Wai Wai. "TRAF3 regulates B cell survival and IL-6 receptor signaling." Diss., University of Iowa, 2015. https://ir.uiowa.edu/etd/1678.
Повний текст джерелаEdouard, Thomas. "Impact sur la signalisation cellulaire des mutations de la tyrosine phosphatase Shp2 associées aux syndromes de Noonan et LEOPARD." Toulouse 3, 2009. http://thesesups.ups-tlse.fr/849/.
Повний текст джерелаNoonan syndrome (NS) is a relatively frequent (about 1/2000 births) autosomal dominant disease primarily characterized by facial dysmorphism, heart defects and short stature. LEOPARD syndrome (LS) is a rarer but related disorder that associates, roughly, NS symptoms with deafness and cutaneous abnormalities. Both NS and LS belong to the family of "neuro-cardio-facial-cutaneous" (NCFC) syndromes, a group of developmental disorders, which display different combinations of the above-mentioned symptoms with mental retardation and tumor predisposition. At least 80% of LS and 50% of NS patients carry germline missense mutations in PTPN11, the gene encoding Shp2. Shp2 is a widely expressed protein tyrosine phosphatase (PTP) that contains Src homology 2 (SH2) domains and promotes Ras-MAPK activation through different molecular mechanisms. Biochemical studies have shown that NS mutations are located at contact points between the catalytic and the SH2 domains and therefore disrupt Shp2 autoinhibitory conformation, stimulating Shp2 catalytic activity (gain-of-function mutations). Conversely, LS mutations are confined within the catalytic domain and repress Shp2 activity. Although genetic studies provided essential advances, how PTPN11 mutations cause the diseases' symptoms remains an open question. We assessed whether LS mutations could influence PI3K activation. To this aim we generated primary and immortalized fibroblast cell lines from LS patient and healthy controls and showed that, in response to EGF stimulation, PI3K/Akt was upregulated in LS cells. This deregulation was due to impaired dephosphorylation of Gab1 PI3K-binding sites by LS mutants. Furthermore, LS mutant promoted PI3K-dependent upregulation of hypertrophy genes in cardiomyocytes, suggesting that this deregulation is involved in LS pathophysiology
Miraldi, Emily R. (Emily Rae). "Bridging the gap between protein-tyrosine phosphorylation networks, metabolism and physiology in liver-specific PTP1b deletion mice." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/72824.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references.
Metabolic syndrome describes a complex set of obesity-related disorders that enhance diabetes, cardiovascular, and mortality risk. Studies of liver-specific protein-tyrosine phosphatase lb (PTPlb) deletion mice (L-PTPlb-/-) suggests that hepatic PTPlb inhibition would mitigate metabolic syndrome progression through amelioration of hepatic insulin resistance, endoplasmic reticulum stress, and whole-body lipid metabolism. However, the network alterations underlying these phenotypes are poorly understood. Mass spectrometry was used to quantitatively discover protein phosphotyrosine network changes in L-PTP lb-/- mice relative to control mice under both normal and high-fat diet conditions. A phosphosite set enrichment analysis was developed to identify numerous pathways exhibiting PTPlb- and diet-dependent phosphotyrosine regulation. Detection of PTP lb-dependent phosphotyrosine sites on lipid metabolic proteins initiated global lipidomics characterization of corresponding liver samples and revealed altered fatty acid and triglyceride metabolism in L-PTPlb-/- mice. Multivariate modeling techniques were developed to infer molecular dependencies between phosphosites and lipid metabolic changes, resulting in quantitatively predictive phenotypic models.
by Emily R. Miraldi.
Ph.D.
Owen, Carl. "The role of adipocyte and liver protein tyrosine phosphatase 1B (PTP1B) in glucose homeostasis and insulin sensitivity." Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203411.
Повний текст джерелаHirata, Aparecida Emiko. "Modulação da associação IR/PTP1B na transmissão do sinal da insulina em modelos animais de resistencia insulinica." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311229.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: A PTP18 atua como efetor negativo do receptor da insulina, desfosforilando o receptor e o IRS-1 e parece estar correlacionada com a sensibilidade à insulina e desenvolvimento da obesidade. No presente estudo avaliamos a associação do receptor da insulina com a PTP18 sobre a regulação da transmissão do sinal da insulina em animais obesos MSG, animais submetidos a jejum por 72h e animais tratados agudamente com adrenalina....Observação: O resumo, na integra, podera ser visualizado no texto completo da tese digital
Abstract: Insulin is the most potent anabolic hormone known and is essential for appropriate tissue development, growth, and maintenance of whole-body glucose homeostasis. Insulin signaling is initiated by binding of insulin to the insulin receptor (IR), stimulating its tyrosine kinase activity, which, in tum triggers downstream signaling events. These include tyrosil phosphorylation of IR substrates 1 and 2 (IRS-1, IRS-2) that activate other adapter molecules such as PI3-K, PDK1 and Akt, which combined actions mediate the biological effects of insulin....Note: The complete abstract is available with the full electronic digital thesis or dissertations
Doutorado
Doutor em Clínica Médica
Richan, Teisha. "Conservative Tryptophan Mutations in Protein Tyrosine Phosphatase PTP1B and its Effect on Catalytic Rate and Chemical Reaction." DigitalCommons@USU, 2017. https://digitalcommons.usu.edu/etd/5584.
Повний текст джерела