Дисертації з теми "Protoplasts"
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Lynch, Paul Thomas. "The uptake of fungal protoplasts by plant protoplasts." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328131.
Повний текст джерелаMullins, P. J. "Protoplasts from Phytophthora." Thesis, University of Liverpool, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381366.
Повний текст джерелаLawrence, W. A. "Microinjection of tobacco protoplasts." Thesis, University of East Anglia, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372559.
Повний текст джерелаAttree, S. M. "Properties of Pteridium protoplasts." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378009.
Повний текст джерелаSheard, J. P. "Glucose uptake by pea mesophyll protoplasts." Thesis, University of East Anglia, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235210.
Повний текст джерелаHansen, Christine S. "Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27935.
Повний текст джерелаLand and Food Systems, Faculty of
Graduate
Souza, Bárbara Lizandra Perini de. "Fusão de protoplastos entre Penicillium echinulatum e Trichoderma harzianum para obtenção de variabilidade visando a produção de celulases." reponame:Repositório Institucional da UCS, 2007. https://repositorio.ucs.br/handle/11338/881.
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The study of cellulolytic fungi has proved to be important, considering economic interest of the cellulase complex, especially in the textile industry and, more recently, for energy purposes. In this work, the protoplast fusion was used to combine genotypes of mutants partially non repressed for cellulases production of Penicillium echinulatum (9A02S1B9) and Trichoderma harzianum (AS5CH3) using the technique dead donor, intending to obtain recombinants with higher cellulases production. In this strategy, both strains had their mycelium treated with Glucanex 0,01 g/mL, to lyse the cell wall. The protoplast obtained from the benomyl-resistant (9A02S1B9) were heat-inactivated (technique of dead donor) at 60ºC, before the step of fusion, induced by PEG4000 and Ca2+, with protoplast of the sensitive-benomyl strain (AS5CH3). Twenty four sub-clones were selected from one fusion product, after stabilization and selection strategies for precocity and efficiency in the formation clearing zones of by cellulose hydrolysis in Petri plates. The fusion products showed similar morphology and sporulation to one of parents, thirteen similar to Penicillium, nine similar to Trichoderma and two showed altered forms. The fusion products which segregate to the morphology of T. harzianum resistance to benomyl, being able to grow and sporulate in media containing up to 100 μg/mL of this inhibitor. The morphology, the profile of bands, obtained by RAPD, and the pattern of cellulase secretion by fusion products were ever more similar to one of parents. The fusants presented variation in the halo of cellulose hydrolysis in Petri plates, and in the activity on filter paper (FPAases), - glicosidase or endoglicanase, when grown submerged cultivation or solid state. From this variability, significant improvement was verified for some of the parental strains. The application of the protoplast fusion methodology to obtain recombinant between P. echinulatum and T. harzianum, using the technique of dead donor, has proved to be adequate to generate variability in the production of cellulases.
Bourne, N. "The regeneration and transformation of Bacillus protoplasts." Thesis, Cardiff University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376554.
Повний текст джерелаNewell, Jane Marie. "Vacuole development in evacuolated oat mesophyll protoplasts." Thesis, University of Southampton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295919.
Повний текст джерелаWard, Kenneth Glenn. "Microinjection and regeneration of tobacco and potato protoplasts." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303971.
Повний текст джерелаDoulis, Andreas G. "Antioxidant responses of pea (Pisum sativum L.) protoplasts." Diss., This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-09192008-063125/.
Повний текст джерелаKuroki, Mayra Akemi. "Desenvolvimento de uma estratégia de clonagem customizada de regiões promotoras do genoma da cana-de-açúcar." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-082130/.
Повний текст джерелаThe aim of this work is develop a strategy to identify functional promoter regions from any genome. The modern sugarcane genome was chosen as a model for the development of this strategy that involves the generation of fragments of DNA and cloning them into expression vectors. These fragments were then screened by a transient expression assay using biolistic particle delivery resulting in the isolation of four clones. Three clones were permanently transformed in rice, and 12 plants were obtained. GUS expression was detected in the callus, leaves and roots of the rice plants thus confirming the functionality of sequences in these clones. The present work has established a strategy to identify and extract functional regulatory sequences containing functional regulatory regions which show great potential of being useful in both the biotechnology field and in the field of basic science.
Hörtensteiner, Stefan. "Re-formation of vacuoles in evacuolated tobacco mesophyll protoplasts /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10426.
Повний текст джерелаDe, Both Michiel Theodoor Jan. "Gene transfer by electroporation in plant protoplasts and tissues." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46278.
Повний текст джерелаButt, Adrian David. "Physiological and biochemical studies with rubber (Hevea brasiliensis) protoplasts." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257197.
Повний текст джерелаSproule, Anne Carleton University Dissertation Biology. "Plant regeneration from stem cortex protoplasts of Brassica juncea." Ottawa, 1987.
Знайти повний текст джерелаCrotty, Christopher M. "Two distinct outward K+ conductances are simultaneously activated in TBY-2 suspension culture protoplasts." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38174.
Повний текст джерелаBoth components were highly K+-selective, however the tail current amplitudes of the slowly activating component at hyperpolarized potentials exhibited non-linear rectification whereas the tail current amplitudes of the fast activating component were linear. The ratio of inward tail current/activated outward current (envelope of tails test) was not constant during the depolarizing step; during the first 50--100 milliseconds the ratio was 6 times higher than at quasi-steady-state (i.e. after 0.3 second).
A pharmacological dissection of outward currents revealed that external Ba2+ in the range from 10 muM to 1 mM selectively inhibited a fast, sigmoidally activating, slowly inactivating current as revealed by examining difference currents. The more slowly activating component was inhibited by only 20% with 5 mM Ba2+. Conversely, nitrendipine or bepridil (5--100 muM) selectively inhibited the slower component of outward current. External TEA inhibited both the fast and slow components equally; tail current amplitudes of both components were inhibited by 40% with 2 mM TEA and the activation time courses in the presence of TEA conserved the same kinetic parameters as control currents.
Foreman, Julia Louise. "Genetic and molecular analysis of root hair initiation and tip growth in Arabidopsis thaliana." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247111.
Повний текст джерелаLamb, Mary Patricia. "Osmolality tolerance and ion channels in protoplasts of entomophthoralean fungi." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0006/MQ34198.pdf.
Повний текст джерелаSpice, Nicola Jane. "Evaluating the use of fungal protoplasts to investigate fungicide action." Thesis, Nottingham Trent University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341273.
Повний текст джерелаCurtis, Nicola. "Protoplasts as tools for the genetic study of Agraricus bisporus." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335201.
Повний текст джерелаGeorge, Alexander Christopher Stephen. "Magnetofection of tobacco protoplasts: a novel mechanism for plant transformation." Thesis, George, Alexander Christopher Stephen (2018) Magnetofection of tobacco protoplasts: a novel mechanism for plant transformation. Honours thesis, Murdoch University, 2018. https://researchrepository.murdoch.edu.au/id/eprint/49707/.
Повний текст джерелаSaka, Kamel. "REGENERATION OF COTTON (GOSSYPIUM HIRSUTUM L.) CALLUS PROTOPLASTS TO MACROCALLI." Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275376.
Повний текст джерелаSinha, Debleena. "Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500422/.
Повний текст джерелаYokoya, Kazutomo. "Studies on the regeneration of intergeneric somatic hybrid plants of roses." Thesis, University of East London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360915.
Повний текст джерелаStanworth, Marie Helen. "Plasma membrane ATPase of Phytophthora cactorum." Thesis, University of the West of England, Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284886.
Повний текст джерелаButler, Declan M. "Isolation and culture of protoplasts and tissues of Laminaria spp. (Phaeophyta)." Thesis, University of Leeds, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237096.
Повний текст джерелаSinha, Anupam. "Studies on the isolation and culture of Lupin (Lupinus albus) protoplasts." Thesis, University of Reading, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297700.
Повний текст джерелаAl-Forkan, Mohammad. "Agrobacterium-mediated transformation of Indica rice (Oryza sativa L.)." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342480.
Повний текст джерелаBrowne, H. M. "Protoplasts and their application to the study of genetics in Lipomyces starkeyi." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235343.
Повний текст джерелаBayley, C. C. R. "An assessment of the consequences of fusion of tobacco and alfalfa protoplasts." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376160.
Повний текст джерелаMatthews, Derek. "The use of protoplasts in the regeneration and genetic manipulation of rose." Thesis, University of East London, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385004.
Повний текст джерелаJackson, R. T. "Isolation of plasma membranes from leaf protoplasts : A search for definitive markers." Thesis, University of Southampton, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373954.
Повний текст джерелаSlomka, Marek Jozef. "Studies of tomato golden mosaic virus in plants, protoplasts and tissue culture." Thesis, Imperial College London, 1987. http://hdl.handle.net/10044/1/46616.
Повний текст джерелаEdwards, K. J. "Studies of homologous recombination between plasmid and chromosomal DNA in plant protoplasts." Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/35209.
Повний текст джерелаCollings, Alan. "Cellulase and related enzyme activity in protoplasts and mycelium of filamentous fungi." Thesis, Sheffield Hallam University, 1986. http://shura.shu.ac.uk/19494/.
Повний текст джерелаPenmetsa, Ramachandra V. "Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts." Thesis, This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-09052009-040406/.
Повний текст джерелаAbram, Beverley Louise. "Responses of Nicotiana glauca guard cell protoplasts to changes in the culture environment." Thesis, Lancaster University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314238.
Повний текст джерелаElshihy, O. M. "In vitro culture of tissues, cells and protoplasts of cotton (Gossypium barbadense L.)." Thesis, University of Southampton, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374060.
Повний текст джерелаStarling, Anthony Patrick. "The uptake of heavy metals by protoplasts and whole cells of filamentous fungi." Thesis, Keele University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293925.
Повний текст джерелаValles, K. L. M. "The use lipophilic ions to measure intracellular membrane potentials in intact wheat protoplasts." Thesis, University of Sussex, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373919.
Повний текст джерелаDaniels, Alison. "Microscopic and computer analysis of ultrastructural changes accompanying isolation and manipulation of tobacco protoplasts." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278755.
Повний текст джерелаHarker, C. L. "The detection of virus coded proteins in cauliflower mosaic virus infected plants and protoplasts." Thesis, Bucks New University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376417.
Повний текст джерелаYeung, Elaine Yee-Ling. "Functional roles of group vii erfs through transient transformation of protoplasts in Arabidopsis and rice." Thesis, University of California, Riverside, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=1547838.
Повний текст джерелаEnvironmental stresses involving flooding and the accompanying cellular low oxygen (hypoxia) constitute a major limitation to agricultural production worldwide. These stresses hinder the plant's ability to maintain energy production and growth. Yet, previous studies have shown that certain genes of group VII ethylene responsive factor (ERF) family of transcription factors play a functional role in the survival of submergence and low oxygen stresses in Oryza sativa(rice) and Arabidopsis thaliana. This study utilized a transient gene transfection assay of protoplasts to characterize gene networks regulated by the group VII ERFs by measuring the transcriptional activation of various promoters. Experiments revealed that orthologous genes are regulated by the group VII ERFs of both species. In rice, an ERF-type transcription factor, SUB1A, confers submergence tolerance by regulating carbohydrate metabolism, elongation growth, and hormonal responses. SUB1A is linked to the related genes SUB1B and SUB1C. Here, two alleles of SUB1A (SUB1A-1 , SUB1A-2) as well as one allele each of the other two genes (SUB1B-1 and SUB1C-1) were shown to be transcriptional activators, and the nuclear localization of SUB1A-1 and SUB1C-1 was demonstrated. In Arabidopsis, core hypoxia responsive genes are governed by the N-end rule pathway of targeted proteolysis, in which proteins of the group VII ERFs (i.e., RAP2.12 and HRE2) are regulated post-translationally under hypoxic conditions. Transactivation experiments demonstrated that the trihelix protein HRA1 negatively regulates the transcriptional activation ability of RAP2.12 in the core hypoxia response. By contrast, HRE2 was not targeted by HRA1 downregulation, but was instead upregulated by RAP2.12 during the stress. Experiments involving truncations of HRA1 reveal that the coil-coil domain is functionally important for HRA1 interaction with group VII ERF complexes. These results with a rapid protoplast assay provide a foundation for future investigations into the low oxygen response gene network regulated by group VII ERF transcription factors binding to cis-regulatory elements, and modulated by the trihelix protein HRA1.
Yan-Xiu, Zhao. "The isolation, culture and genetic manipulation of protoplasts from salt and drought tolerant leguminous plants." Thesis, Coventry University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332788.
Повний текст джерелаShacklock, Philip S. "The involvement of second messengers in the phytochrome-mediated swelling of etiolated wheat leaf protoplasts." Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/14380.
Повний текст джерелаAbdullah, R. B. "The development of a reproducible system for efficient plant regeneration from rice (Oryza sativa L.) protoplasts." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381060.
Повний текст джерелаWoskow, Steven A. "Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts and Transformation of Streptococcus cremoris." DigitalCommons@USU, 1987. https://digitalcommons.usu.edu/etd/5357.
Повний текст джерелаGomez, Luengo Rodolfo Gustavo. "Proteins and serological relationships of maize mosaic virus isolates and replication of the virus in Maize (Zea Mays L.) protoplasts /." The Ohio State University, 1987. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487327695621001.
Повний текст джерелаCarvalho, Dayse Cristina de. "Fusão de protoplastos visando a reconstrução da laranja azeda." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-12012007-160405/.
Повний текст джерелаThe aim of this work was to apply the technique of chemical fusion of protoplasts, in order to develop interspecific somatic hybrids between mandarins (Citrus reticulata) and pummelos (Citrus grandis), in order to produce similar to sour orange (Citrus aurantium). The sources for protoplasts were embryogenic suspension cultures of \'Page\' tangelo (C. reticulata x C. paradisi) and \'Murcott\' tangor (C. reticulata x C. sinensis) and young leaves from seedlings of \'Lau Tau\' and \'Ogami\' pummelos (Citrus grandis). After the fusion, the protoplasts were cultivated in the absence of light, until the formation of microcolonies, and were then cultivated in double-phase EME medium, supplemented with 13.33 g/L of maltose for embryogenesis induction. The globular embryos thus formed were transferred to EME medium with 25 g/L of sucrose and, when in cotyledonal stage, were transferred to a culture medium supplemented with 1.5 g/L of malt extract. The shoots obtained were grafted in vitro onto \'Hamlin\' and \'Valencia\' sweet oranges. The regenerated plants were cultivated in a greenhouse, over commercial substrate. In this process, 17 plants were obtained. These plants presented phenotypic conformation different from the genitors, with leaves with reduced size, round apex, dark green coloration, rough leaf blade and absence of developed petiole. The analysis by flow cytometry confirmed the diploid character of the regenerated plants. RAPD molecular markers presented a similar band pattern between the regenerated specimen and the genitor \'Page\' tangelo. The protocol used for isolation, hybridization and cultivation of protoplasts, as well as for the regeneration and acclimatization of the plants allowed the obtainment of 17 plants from the combination of \'Page\' tangelo + \'Lau Tau\' pummelo, with phenotypic conformation different from the genitors, two plants from the combination of \'Murcott\' tangor + \'Ogami\' pummelo and one plant from the combination of Murcote\' tangor + \'Lau Tau\' pummelo.