Дисертації з теми "Protocol characterization"
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Parel, Ilaria. "Validation and application of a shoulder ambulatory motion analysis protocol." Doctoral thesis, Università degli studi di Trieste, 2013. http://hdl.handle.net/10077/8530.
Повний текст джерелаLe principali attività di ricerca svolte durante il dottorato hanno riguardano la validazione e caratterizzazione dell’applicabilità di un protocollo per l’analisi della cinematica di spalla in ambito clinico (ISEO - INAIL Shoulder and Elbow Outpatient protocol). Lo scopo principale era quello di creare uno strumento che fornisse al personale sanitario informazioni sulla performance motoria dei pazienti, supportando, con informazioni di tipo quantitativo, la valutazione ambulatoriale delle patologie della spalla. E’ possibile suddividere l’attività di ricerca in tre temi principali: caratterizzazione e validazione di ISEO; applicazione di ISEO per valutazioni di tipo clinico; applicazione di ISEO per valutazione di performance motoria in ambito sportivo. Grazie ai processi di validazione e caratterizzazione svolti e alle applicazioni di ISEO, ad oggi il protocollo può essere utilizzato in studi clinici e sportivi riguardanti la cinematica di spalla (coordinazione scapolo-omerale), per i quali la sensibilità dello strumento può essere considerata adatta alle esigenze valutative.
The main research activities carried out during the PhD were related to the validation and characterization of the applicability of a protocol for the analysis of the kinematics of the shoulder in a clinical setting (ISEO - Shoulder and Elbow INAIL Outpatient protocol). The main purpose was to create a tool that provides quantitative information about the motor performance of patients, supporting clinicians for the assessment of ambulatory shoulder disorders. The research activity can be split in three main themes: characterization and validation od ISEO; application of ISEO for clinical assessments; application of ISEO for sport performance assessments. Thanks to the validation and characterization of the protocol and its application in several contests, it can be concluded that ISEO can be used to evaluate the kinematics of the shoulder (in particular the scapulohumeral coordination) in clinical and sport performance studies, for which the sensitivity of the protocol can be considered appropriate.
XXV Ciclo
1983
BREMBILLA, GIORGIO. "Simplification of prostate MRI protocol and development of a novel MRI technique for prostate cancer detection and characterization." Doctoral thesis, Università Vita-Salute San Raffaele, 2022. http://hdl.handle.net/20.500.11768/133075.
Повний текст джерелаBackground e razionale: La risonanza magnetica mutiparametrica (mpMRI) è la modalità diagnostica di scelta per la diagnosi del tumore prostatico. Tuttavia, la mpMRI ha diverse limitazioni, tra cui la limitata accessibilità, l'alta variabilità inter-osservatore, la bassa specificità e il basso valore predittivo positivo. Scopo dello studio: Con particolare riferimento alla possibilità di fare fronte alle correnti limitazioni della mpMRI, lo scopo dello studio è quello di valutare la reale variabilità inter-osservatore della mpMRI, di valutare la fattibilità di una semplificazione dei protocolli di RM prostatica, e di sviluppare una nuova tecnica RM chiamata Luminal Index MRI (LI-MRI). Materiali e Metodi: I) La reale variabilità inter-osservatore è stata valutata in uno studio multi-osservatore progettato per rispecchiare il più possibile la normale pratica quotidiana, su un campione di 200 pazienti. Sette radiologi hanno rivalutato le risonanze secondo i criteri PI-RADS v2.1. Si è valutata la concordanza sulla identificazione della lesione principale (Index Lesion) mediante il coefficiente K di Conger, il coefficiente di agreement 1 (AC1), le percentuali di concordanza e gli indici di concordanza specifici. II) La fattibilità di un protocollo semplificato di RM prostatica è stata testata su 151 pazienti che avessero effettuato mpMRI e mappaggio prostatico mediante biopsie transperineali (TTPM). Tre radiologi esperti hanno rivalutato le immagini utilizzando tre diversi protocolli (risonanza multiparametrica (mpMRI), biparametrica (bpMRI) e biparametrica abbreviata (a-bpMRI)). È stata valutata la performance diagnostica per ogni protocollo e la concordanza inter-osservatore. III) La performance diagnostica della LI-MRI è stata testata su 178 pazienti che avessero fatto una mpMRI e LI-MRI per sospetto tumore prostatico. I risultati ottenuti sono stati validati su una coorte di valutazione, oltre alla riproducibilità della metodica su scansioni ripetute. IV) Gli esami di LI-MRI sono stati rivalutati retrospettivamente in confronto alla mpMRI e a i risultati delle biopsie per sviluppare un sistema di interpretazione ad hoc per LI-MRI. Risultati: I) La concordanza sulla identificazione della Index Lesion è stata sostanziale (AC1 0.738; 95% CI 0.695–0.782), maggiore per i radiologi esperti nei confronti dei meno esperti. II) La sensibilità e la specificità della a-bpMRI è stata di 92% e 48%, rispettivamente, senza significativa differenza nei valori di sensibilità se confrontata con la bpMRI e mpMRI. La concordanza interosservatore per a-bpMRI è però inferiore (AC1 0.58). III) La tecnica LI-MRI che utilizza un protocollo semplificato è fattibile, riproducibile e ottiene ottimi valori di sensibilità e specificità per la identificazione del tumore prostatico (65-78% specificità per 89-90% sensibilità). IV) Uno scoring system dedicato è stato sviluppato per la tecnica LI-MRI e basato sull'interpretazione delle sequenze T2-multieco delle mappe di luminal index. Conclusioni: La variabilità inter-osservatore della risonanza magnetica è inferiore a quanto precedentemente descritto dalla letteratura. I protocolli abbreviati di RM prostatica sono efficaci quanto quelli standard (se interpretati da lettori esperti) e dovrebbero essere presi in considerazione per aumentare l'accessibilità alla RM prostatica. La tecnica LI-MRI ha ottenuto risultati promettenti e potrebbe migliorare la caratterizzazione del tumore prostatico utilizzando la risonanza magnetica.
Anctil, Jean-Claude. "Experimental characterization of a low-dose-rate and a high-dose-rate iridium-192 brachytherapy source using the AAPM TG 43 dosimetry protocol." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape15/PQDD_0003/MQ37088.pdf.
Повний текст джерелаAnctil, Jean-Claude. "Experimental characterization of a low dose-rate and a high dose-rate iridium-192 brachytherapy source using the AAPM TG 43 dosimetry protocol." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28232.
Повний текст джерелаDose measurements have been performed using lithium fluoride thermoluminescent detectors positioned in a polystyrene phantom at distances from the source that vary from 1 cm to 10 cm, with 1-cm intervals, and at angles that vary from 0$ sp circ$ to 170$ sp circ$ with 10$ sp circ$ intervals.
Our experimental results have clearly shown that the point-source approximation model can overestimate the dose to water, especially for the high dose-rate source, where we have found that differences between point-source estimates and exact measured values can differ by almost 30% for points along the longitudinal axis of the source.
Gerard, Bengua. "Evaluation of the near threshold 7Li(p,n)7Be accelerator-based irradiation system for BNCT : a treatable protocol depth (TPD)-based characterization of neutron fields." 京都大学 (Kyoto University), 2007. http://hdl.handle.net/2433/136226.
Повний текст джерелаDeschamps, Alice. "Characterization of modern reefs using the Atlantic and Gulf Rapid Reef Assessment (AGRRA) protocol and digitized aerial photographs, Tobago Cays Marine Park, St. Vincent and the Grenadines." Thesis, University of Ottawa (Canada), 2000. http://hdl.handle.net/10393/8613.
Повний текст джерелаBhattacharjee, Sudip. "Use of accelerated loading equipment for fatigue characterization of hot mix asphalt in the laboratory." Link to electronic thesis, 2005. http://www.wpi.edu/Pubs/ETD/Available/etd-01075-124314/.
Повний текст джерелаKeywords: characterization; test protocol; finite element method; performance curve; thermocouple; rutting; strain gauge; MMLS3; fatigue; instrumentation. Includes bibliographical references (p. 117-119).
Tran, Thi Ngoc Tuyen [Verfasser], Herbert [Akademischer Betreuer] Waldmann, and Martin [Gutachter] Engelhard. "Evaluation of the phage display protocol for target identification of small molecules : Identification and characterization of mitosis modulators / Thi Ngoc Tuyen Tran. Betreuer: Herbert Waldmann. Gutachter: Martin Engelhard." Dortmund : Universitätsbibliothek Dortmund, 2014. http://d-nb.info/1101595531/34.
Повний текст джерелаHaba, Steven R. "Conservation of Begonia germplasm through seeds: characterization of germination and vigor in different species." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1420040181.
Повний текст джерелаO'Donoghue, Peter John. "Characterization of parasitic protozoa in Australia /." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe.pdf.
Повний текст джерелаGOWRISANKAR, SIVAKUMAR. "ANALYSIS AND CHARACTERIZATION OF PARALLEL MIXED-SIGNAL SYNCHRONIZATION PROTOCOLS." University of Cincinnati / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1108493593.
Повний текст джерелаCARUSO, VALENTINA. "DEGRADATION OF ORGANIC AND MINERAL PHASES IN BURIED HUMAN REMAINS: THE EARTH SCIENCES ANALYTICAL CHARACTERIZATION." Doctoral thesis, Università degli Studi di Milano, 2017. http://hdl.handle.net/2434/478504.
Повний текст джерелаCorreia, Ricardo Miguel Paulino Barroso Dias. "Synthesis and characterization of sugar containing hydrophilic and hydrophobic polymers with potential application in medicine." Master's thesis, Faculdade de Ciências e Tecnologia, 2013. http://hdl.handle.net/10362/11082.
Повний текст джерелаThere has been a worldwide acknowledgement that nature derived saccharides can provide the raw materials needed for the production of numerous industrial consumer goods. As such, sucrose is a low molecular weight renewable carbohydrate feedstock from which it is possible to elaborate new materials, like water-soluble and/or amphiphilic and biocompatible polymers. In this thesis we will describe some synthetic procedures (both conventional synthesis protocols (CSP) and microwave assisted protocols (MAPs)) by introducing and altering sugar hydroxyl groups, with the intent to produce functionalized polymers for use as biodegradable/biocompatible polymers with sugar linked side chains. The most widely used method for the synthesis of poly(vinyl saccharide)s has been based on free radical polymerizations of vinyl sugars. In this work, eleven compounds based on sucrose derivatization were synthesized using anhydrides, bromide halides, silyl chlorides, non-selective esterification and Mitsunobu reaction. Optimization and scale-up studies were made on monomer synthesis. Four of these compounds were used as monomers for radical copolymerization with styrene using as catalysts 2,2’-Azobis(2-methylpropionitrile) and sodium persulfate whether organic solvents or water was used as reaction media. From this copolymerization’s, four polymers were obtained and polystyrene was also synthesized to be used as a standard for comparison. The polymers, poly(1’,2,3,3’,4,4’,6-hepta-O-benzyl-6’-O-methacryloyl sucrose)-co-polystyrene, poly(1’,2,3,3’,4,4’,6’-hepta-O-acetyl-6-O-methacryloyl sucrose)-co-polystyrene, poly(6-O-methacryloyl sucrose)-co-polystyrene and poly(O-methacryloyl sucrose)-co-polystyrene, were characterized by Proton nuclear magnetic resonance (to assess sucrose vinyl ester/styrene ratio), Fourier transform infrared spectroscopy, Differential scanning calorimetry, Powder X-ray diffraction, Atomic force microscopy (topology studies as thin films and aggregates), Viscometry and polarimetry.
VILLA, LUCA. "EPIDEMIOLOGY AND MOLECULAR CHARACTERIZATION OF SELECTED PROTOZOA IN DOMESTIC RUMINANTS." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/809642.
Повний текст джерелаVirel, Ana. "Molecular characterization and evolution of alpha-actinin : from protozoa to vertebrates." Doctoral thesis, Umeå University, Chemistry, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-931.
Повний текст джерелаalpha-actinin is a ubiquitous protein found in most eukaryotic organisms. The ability to form dimers allows alpha-actinin to cross-link actin in different structures. In muscle cells alpha-actinin is found at the Z-disk of sarcomeres. In non-muscle cells alpha-actinin is found in zonula adherens or focal adhesion sites where it can bind actin to the plasma membrane.
alpha-actinin is the shortest member of the spectrin superfamily of proteins which also includes spectrin, dystrophin and utrophin. Several hypotheses suggest that alpha-actinin is the ancestor of this superfamily.
The structure of alpha-actinin in higher organisms has been well characterized consisting of three main domains: an N-terminal actin-binding domain with two calponin homology domains, a central rod domain with four spectrin repeats and a C-terminal calcium-binding domain. Data mining of genomes from diverse organisms has made possible the discovery of new and atypical alpha-actinin isoforms that have not been characterized yet.
Invertebrates contain a single alpha-actinin isoform, whereas most of the vertebrates contain four. These four isoforms can be broadly classified in two groups, muscle isoforms and non-muscle isoforms. Muscle isoforms bind actin in a calcium independent manner whereas non-muscle isoforms bind actin in a calcium-dependent manner.
Some of the protozoa and fungi isoforms are atypical in that they contain fewer spectrin repeats in the rod domain. We have purified and characterized two ancestral alpha-actinins from the parasite Entamoeba histolytica. Our results show that despite the shorter rod domain they conserve the most important functions of modern alpha-actinin such as actin-bundling formation and calcium-binding regulation. Therefore it is suggested that they are genuine alpha-actinins.
The phylogenetic tree of alpha-actinin shows that the four different alpha-actinin isoforms appeared after the vertebrate-invertebrate split as a result of two rounds of genome duplication. The atypical alpha-actinin isoforms are placed as the most divergent isoforms suggesting that they are ancestral isoforms. We also propose that the most ancestral alpha-actinin contained a single repeat in its rod domain. After a first intragene duplication alpha-actinin with two spectrin repeats were created and a second intragene duplication gave rise to modern alpha-actinins with four spectrin repeats.
Ochaya, Stephen O. "Mapping the genome and characterization of an acetyltransferase of Trypanosoma cruzi /." Stockholm, 2006. http://diss.kib.ki.se/20060523/ocha/.
Повний текст джерелаSantamaria, Johanna. "Characterization of Protozoa Transport and Occurrence of Chlorinated-Ethene Reducer Bacteria in Subsurface Environments." Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/194620.
Повний текст джерелаButton, Bradly K. "Development of resist free fabrication protocols and performance characterization of micro devices based on metal oxide nanowires /." Available to subscribers only, 2006. http://proquest.umi.com/pqdweb?did=1280149851&sid=19&Fmt=2&clientId=1509&RQT=309&VName=PQD.
Повний текст джерелаAlmeida, André Augusto da Silva. "Cryptosporidium spp and Giardia duodenalis: protozoa transmitted through water. Detection and genetic characterization - new approaches." Doctoral thesis, Instituto de Ciências Biomédicas Abel Salazar, 2009. http://hdl.handle.net/10216/24612.
Повний текст джерелаAlmeida, André Augusto da Silva. "Cryptosporidium spp and Giardia duodenalis: protozoa transmitted through water. Detection and genetic characterization - new approaches." Tese, Instituto de Ciências Biomédicas Abel Salazar, 2009. http://hdl.handle.net/10216/24612.
Повний текст джерелаAnkarklev, Johan. "Inter and Intra-Assemblage Characterizations of Giardia intestinalis: from clinic to genome." Doctoral thesis, Uppsala universitet, Institutionen för cell- och molekylärbiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-167063.
Повний текст джерелаRuiz, Fuertes María Idoia. "On the Consistency, Characterization, Adaptability and Integrity of Database Replication Systems." Doctoral thesis, Universitat Politècnica de València, 2011. http://hdl.handle.net/10251/11800.
Повний текст джерелаRuiz Fuertes, MI. (2011). On the Consistency, Characterization, Adaptability and Integrity of Database Replication Systems [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/11800
Palancia
Podesta', A. "Development of protocols for a quantitative characterization of morphological and tribological properties of nanostructured films via the atomic force microscope." Doctoral thesis, Università degli Studi di Milano, 2002. http://hdl.handle.net/2434/72374.
Повний текст джерелаAlarady, Mamdooh R. "Characterization of Image Quality between Multi-Slice Computed Tomography and Cone Beam Computed Tomography for Clinical Used Protocols in Radiation Therapy Treatment Planning." University of Toledo Health Science Campus / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=mco151080400269082.
Повний текст джерелаJafari, Leila. "The effect of tissue mechanical characterization and stimulation parameters on live tissue mechanobiological progression with regard to viscoelasticity and viscoplasticity." Thèse, Université de Sherbrooke, 2012. http://hdl.handle.net/11143/6130.
Повний текст джерелаEtinosa, Omoruyi Beauty. "Immunological and molecular characterization of Cryptosporidium species in HIV-Positive and HIV-Negative diarrhoea patients in the Nkonkobe Municipality of the Eastern Cape Province of South Africa: a pilot study." Thesis, University of Fort Hare, 2010. http://hdl.handle.net/10353/392.
Повний текст джерелаAgasid, Mark Tadashi, and Mark Tadashi Agasid. "I. Development of Rapid Conductance-Based Protocols for Measuring Ion Channel Activity; II. Expression, Characterization, and Purification of the ATP-Sensitive, Inwardly-Rectifying K+ Channel, Kir6.2, and Ion Channel-Coupled Receptors." Diss., The University of Arizona, 2017. http://hdl.handle.net/10150/623173.
Повний текст джерелаROTA, GRAZIOSI ANDREA. "EVALUATION AND CHARACTERIZATION OF DIETARY STRATEGIES ON ENVIRONMENTAL SUSTAINABILITY OF DAIRY COW MILK PRODUCTION." Doctoral thesis, Università degli Studi di Milano, 2022. http://hdl.handle.net/2434/924352.
Повний текст джерелаCardoso, Micaela 1988. "Produção e caracterização de lesões artificiais de cárie em esmalte, similares às do protocolo ICDAS II, por biofilme bacteriando de Streptococcus mutans : Production and characterization of enamel caries lesion similar to ICDAS II criteria by Streptococcus mutans biofilm." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288582.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-24T18:37:20Z (GMT). No. of bitstreams: 1 Cardoso_Micaela_M.pdf: 3863451 bytes, checksum: 498ee01fbebe667f482927f69b67f6d4 (MD5) Previous issue date: 2014
Resumo: Este estudo teve por objetivos: produzir lesões artificiais de cárie em esmalte bovino, com características clínicas similares àquelas identificadas pelo protocolo ICDAS II 2009 (International Caries Detection and Assessment System), códigos 1, 2, e 3, através da inspeção visual; identificar o tempo de desmineralização necessário para a produção de lesões artificiais de cárie em esmalte bovino; caracterizar segundo a área de secção transversal, profundidade (LD) e volume da lesão, topografia da superfície, a razão entre a porcentagem de perda mineral e a profundidade da lesão (ratio) e perda mineral das lesões, por meio de diferentes metodologias de análise: Análise visual, Microtomografia (?-CT) Microscopia Eletrônica de Varredura (MEV), Microrradiografia Transversal (TMR) e Microscopia óptica em Luz Polarizada (MLP). Foram utilizados 45 blocos de esmalte bovino distribuídos em 3 grupos (n=15), de acordo com o Código ICDAS 1, 2 e 3, e submetidos ao desafio ácido pelo método biológico, com S. mutans. Os blocos foram imersos em meio BHI suplementado por sacarose a 1% com inóculo de S. mutans, sendo o meio trocado a cada 24h, e para identificação do tempo de produção das lesões (estudo piloto). Em seguida, os espécimes foram removidos do meio de cultura de acordo com o período de tempo identificado para cada grupo (24h, 4 dias e 14 dias), fotografados (análise visual), submetidos à análise por ?-CT, MEV e posteriormente, seccionados longitudinalmente em relação à lesão de cárie e avaliados em TMR e MLP. Os dados obtidos da MEV foram avaliados descritivamente; aqueles provenientes da ?-CT, TMR e MLP foram submetidos aos testes ANOVA e Tukey (p<0,05). O tempo necessário para produção de lesões clinicamente semelhantes àquelas para cada código ICDAS II foi identificado visualmente. Para a produção de lesões in vitro similares ao código 1, o tempo necessário foi de 24 horas; para o código 2, 4 dias; e para o código 3, 14 dias. Para as lesões similares àquelas Código 1, a MLP e TMR identificaram a presença de lesões subsuperficiais de cárie, porém o ?-CT não permitiu visualizá-las. O MEV mostrou superfície mais regular, na maioria das amostras, sem sinais de erosão e com pequenos orifícios provocados pelo S. mutans. Para as lesões Código 2 a análise por ?-CT identificou áreas e volume de lesão menores do que para o Código 3. A profundidade da lesão não pode ser identificada pelo ?-CT para ambos os Códigos. MLP e TMR identificaram maiores profundidades de lesão para o Código 3, comparado ao 2, porém, evidenciaram a presença de desmineralização e cavitação, também maiores no grupo ICDAS 3. A profundidade média das lesões dos três grupos foi mensurada pelos métodos TMR e MLP, e ambas as análises evidenciaram que, quanto maior o código ICDAS, maior a profundidade das lesões; porém, não foi observada correlação significativa entre estes dois métodos. Baseando-se nos resultados obtidos pode-se concluir que o método biológico de produção de cárie por S. mutans possibilita a produção de lesões similares às observadas clinicamente pelo protocolo ICDAS II. Diferentes metodologias empregadas podem ser recomendadas de acordo com o tipo de lesão produzida artificialmente
Abstract: This study was developed in order to identify the effect of demineralization process to provide bovine enamel caries-like lesions, using Streptococcus mutans biofilms with clinical features similar to those found in ICDAS II (2009) criteria, codes 1, 2 and 3 by visual inspection; to identify the time required for providing the demineralization; to characterize the lesions obtained from different analysis methodologies: X-ray microtomography (?-CT), Scanning Electron Microscopy (SEM), Transversal Microradiography (TMR) and light polarized optical microscopy (MLP). It was used 45 enamel block divided into 3 groups (n =15) submitted to caries-like lesions by biological method, with Streptococcus mutans biofilm. The specimens were immersed in BHI broth supplemented with 1% sucrose inoculum of S. mutans and the medium was changed every 24 hours. Then, the specimens were removed from the culture media in each period of time (24h, 4 and 14 days) and pictures were taken from their demineralized surface (visual analysis). Next, the specimens were submitted to ?-CT and SEM evaluations and longitudinally sectioned by the caries lesions and they were evaluated in TMR e MLP. Data obtained from SEM were descriptively evaluated. Data from ?-CT, TMR and MLP were submitted to ANOVA and Tukey's tests (p<0,05). The time required to produce clinical lesions similar to those ICDAS II for each protocol code was visually identified. For the production of lesions in vitro similar to code 1, the time required was 24 hours, code 2 for 4 days and code 3 for 14. For the similar lesion to those from code 1, the MLP and TMR identified the presence of subsurface carious lesions, but ??CT did not allow viewing it. The SEM analysis showed smoother surface, mostly without signs of erosion and pinholes caused by S mutans. For code 2 lesions the ??CT analysis identified lesions areas and volume smaller than Code 3. The lesion depth cannot be identified by ??CT for both codes. MLP and TMR identified higher depths to code 3 as compared to 2, however, showed the presence of demineralization and cavitation also higher than code 3 ICDAS. The lesion depth average from the three groups was measured by TMR and MLP methods, and both analyses showed that the higher the ICDAS II code, the deeper the lesions were. Different methods may be employed in accordance with the recommended type of lesion produced artificially
Mestrado
Odontopediatria
Mestra em Odontologia
Kaligora, Koffi. "Caractérisation du comportement mécanique des oxydes sous stoechiometriques sous températures et atmosphères controlées." Thesis, Orléans, 2018. http://www.theses.fr/2018ORLE2062.
Повний текст джерелаThis thesis deals with the development of mechanical set up that allows characterizing the oxygen partial pressure effect on the mechanical behavior of sub-stoichiometric perovskites oxides, used due to their potential oxygen semi-permeation properties. In order to study these materials, a new experimental set up and a new post-process routine are developed. Diametric compression tests and creep by diametric compression are conducted in an original mechanical test furnace with reinforced sealing and controlled atmospheres. The tests are instrumented by optical measurements. In order to control the oxygen partial pressure in the test zone, and to study its real influence on mechanical properties, an oxygen sensor is monitored to the device. To analyze experiences, a new post-process routine called DigImCo.v2 is developed that permits to identify material parameters. This approach combine Digital Image Correlation method and an inverse identification method. The optimization technic is based on Levenberg-Marquardt module and the numeric model simulation of the tests are performed on Abaqus software. For the creep parameters identification, Norton creep model is simulated in Abaqus. Tests results reflect the relatively weak influence of oxygen partial pressure on studies materials, which exhibit negligible creep strains compared to literature
Denecker, Thomas. "Bioinformatique et analyse de données multiomiques : principes et applications chez les levures pathogènes Candida glabrata et Candida albicans Functional networks of co-expressed genes to explore iron homeostasis processes in the pathogenic yeast Candida glabrata Efficient, quick and easy-to-use DNA replication timing analysis with START-R suite FAIR_Bioinfo: a turnkey training course and protocol for reproducible computational biology Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to assess data reproducibility Rendre ses projets R plus accessibles grâce à Shiny Pixel: a content management platform for quantitative omics data Empowering the detection of ChIP-seq "basic peaks" (bPeaks) in small eukaryotic genomes with a web user-interactive interface A hypothesis-driven approach identifies CDK4 and CDK6 inhibitors as candidate drugs for treatments of adrenocortical carcinomas Characterization of the replication timing program of 6 human model cell lines." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASL010.
Повний текст джерелаBiological research is changing. First, studies are often based on quantitative experimental approaches. The analysis and the interpretation of the obtained results thus need computer science and statistics. Also, together with studies focused on isolated biological objects, high throughput experimental technologies allow to capture the functioning of biological systems (identification of components as well as the interactions between them). Very large amounts of data are also available in public databases, freely reusable to solve new open questions. Finally, the data in biological research are heterogeneous (digital data, texts, images, biological sequences, etc.) and stored on multiple supports (paper or digital). Thus, "data analysis" has gradually emerged as a key research issue, and in only ten years, the field of "Bioinformatics" has been significantly changed. Having a large amount of data to answer a biological question is often not the main challenge. The real challenge is the ability of researchers to convert the data into information and then into knowledge. In this context, several biological research projects were addressed in this thesis. The first concerns the study of iron homeostasis in the pathogenic yeast Candida glabrata. The second concerns the systematic investigation of post-translational modifications of proteins in the pathogenic yeast Candida albicans. In these two projects, omics data were used: transcriptomics and proteomics. Appropriate bioinformatics and analysis tools were developed, leading to the emergence of new research hypotheses. Particular and constant attention has also been paid to the question of data reproducibility and sharing of results with the scientific community
Chan, Andrew. "Towards the development and characterization of a selective DNA purification protocol by capillary affinity gel electrophoresis." 2006. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=442006&T=F.
Повний текст джерелаLiu, Wen-Yu, and 劉文裕. "Functional characterization of three ethylene response factor genes from Bupleurum kaoi and an Agrobaterium tumefaciens-mediated transformation protocol." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/75471510311717919949.
Повний текст джерелаGabriel, Leandro de Assis. "Establishment of a multiple particle tracking video microscopy protocol for characterization of nanoparticle transport in complex biological environments." Master's thesis, 2021. https://hdl.handle.net/10216/138522.
Повний текст джерелаAlhassan, Haru. "Performance characterization of the DOCSIS 1.1 HFC Network Protocol with Prioritized First Come First Served (P-FCFS) load scheduling algorithm." 2003. http://hdl.handle.net/1993/19794.
Повний текст джерелаGraça, Kelly de Jesus Allen. "Physiological and biomechanical characterization from low to severe swimming intensities. A study performed using a front crawl intermittent incremental protocol." Doctoral thesis, 2015. https://repositorio-aberto.up.pt/handle/10216/80020.
Повний текст джерелаGraça, Kelly de Jesus Allen. "Physiological and biomechanical characterization from low to severe swimming intensities. A study performed using a front crawl intermittent incremental protocol." Tese, 2015. https://repositorio-aberto.up.pt/handle/10216/80020.
Повний текст джерелаYoungquist, Adam Daniel. "Development of an Accelerated Ash Loading Protocol for Rapid Evaluation of Diesel Particulate Filters Including Comprehensive Characterization of Ash-loaded Substrates." 2008. http://trace.tennessee.edu/utk_gradthes/451.
Повний текст джерелаLu, Chen-Wen, and 盧珍妏. "Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/93qar7.
Повний текст джерела國立臺灣師範大學
生命科學系
105
The aim of the current study was to develop an iron oxide nanoparticle (ION) labelling and magnetic resonance imaging (MRI)-based protocol to allow visualization of the differentiation process of mesenchymal stem cells (MSCs) into neural-like cells (NCs) in vitro. Ferucarbotran, a clinically available ION, which can be visualized under MRI, is used for tracking cells implanted in vivo. The NCs were verified morphologically and histologically by light microscopy, and their functions were verified by measuring their action potentials. Conformational conversion of axon-like structures was observed under light microscopy. These NCs exhibited frequent, active action potentials compared with cells that did not undergo neural differentiation. The labelling of ION had no influence on the morphological and functional differentiation capacity of the MSCs. We conclude that the MSCs that were differentiated into NCs exhibited in vitro activity potential firing and may be used to replace damaged neurons.
LUCHETTI, ALESSANDRA. "The repeated cross fostering protocol as a mouse model of panic disorder: suggestions for new treatments from behavioral and molecular characterization." Doctoral thesis, 2015. http://hdl.handle.net/11573/924267.
Повний текст джерелаSánchez, Cabezas Santiago. "Development of a reproducible and optimized synthetic protocol for the preparation of monodisperse core-shell-type magnetic mesoporous silica nanoparticles." Doctoral thesis, 2019. http://hdl.handle.net/10251/129878.
Повний текст джерела[CAT] La fabricació de nanopartícules amb grandàries per davall dels 100 nm ha permés el desenvolupament d'innovadors nanodispositius capaços d'interactuar de forma directa amb sistemes vius a nivell cel¿lular i molecular, convertint-se en una part fonamental dins del camp de la nanomedicina. Un dels principals reptes als quals s'enfronta l'enginyeria de nanopartícules és el desenvolupament de nanodispositius amb propietats físic-químiques ben definides, ja que d'elles depén el comportament i biodistribució d'aquests sistemes una vegada introduïts en l'organisme. No menys important és el desenvolupament de protocols de síntesis reproduïbles i optimitzats, indispensables per a la fabricació a gran escala de nanodispositius que puguen ser utilitzats en futures aplicacions biomèdiques. El principal objectiu d'aquest projecte de doctorat és l'estudi i fabricació de nanopartícules magnètiques mesoporoses de sílice amb estructura "core-shell" per a la seua aplicació com a agents teranòstics en el camp de la nanomedicina. En aquest estudi s'analitza en profunditat la síntesi i caracterització d'aquests nanomaterials amb l'objectiu de produir nanopartícules amb unes propietats físic-químiques ben definides de forma controlada i reproduïble. L'obtenció d'aquestes nanopartícules suposaria un gran avanç de cara al desenvolupament de nanodispositius més complexos i sofisticats. El contingut de la tesi s'ha estructurat en diferents capítols que es detallen breument a continuació: ¿El capítol 1 és una introducció a la nanomedicina, destacant el paper fonamental que tenen les nanopartícules en el desenvolupament de noves aplicacions biomèdiques. A continuació es presenten les nanopartícules de sílice mesoporosa, mostrant la gran versatilitat d'aquests nanomaterials per al desenvolupament de dispositius teranòstics així com sistemes per a l'alliberament controlat de fàrmacs. Finalment, es destaca la importància de fabricar nanodispositius amb unes propietats físic-químiques ben definides com a requisit indispensable per a la translació dels resultats experimentals al camp clínic. ¿El capítol 2 inclou els objectius principals de la tesi així com els objectius específics proposats per a cada capítol de la tesi. ¿El capítol 3 està dedicat a la síntesi i caracterització de nanopartícules superparamagnétiques d'òxid de ferro (USPIONs), sent aquestes utilitzades en capítols posteriors per a la síntesi de les nanopartícules mesoporoses tipus "core-shell". Les USPIONs són preparades a través d'un mètode senzill de coprecipitació en el qual s'empren condicions de reacció moderades. Les nanopartícules obtingudes són caracteritzades en profunditat, analitzant les seues propietats magnètiques per a la seua aplicació en hipertèrmia magnètica i com a agents de contrast dual en imatge per ressonància magnètica (MRI). ¿El capítol 4 està dedicat a la preparació de nanopartícules magnètiques mesoporoses de sílice amb estructura "core-shell". Els conceptes fonamentals relacionats amb els mecanismes de formació d'aquest tipus de nanomaterials són àmpliament analitzats, així com els paràmetres de reacció involucrats en la síntesi. Com a punt de partida, es proposa un protocol de síntesi general per a l'obtenció de les nanopartícules tipus "core-shell". A continuació, s'analitza en profunditat l'efecte que els diferents paràmetres de reacció tenen en les propietats físic-químiques d'aquestes nanopartícules. Per a la fase d'optimització s'utilitza un model semi-empíric com a referència, racionalitzant els resultats experimentals observats sobre la base d'un possible mecanisme de formació. ¿El capítol 5 està dedicat a l'anàlisi i caracterització de l'estructura mesoporosa de les nanopartícules tipus "core-shell". A més, s'analitza l'efecte que els diferents paràmetres de reacció tenen sobre l'estructura final de les nanopartícules, aportant informació
[EN] The fabrication of nanoparticles with sizes below 100 nm has opened the door to the development of innovative nanodevices that directly interact with living systems at the cellular and molecular level, becoming an essential part of nanomedicine. One of the main challenges that nanoparticle engineering is currently facing is the design of nanodevices with well-defined physico-chemical properties, which ultimately determine the fate and function of these systems inside the organism. Similarly, the development of reproducible and versatile synthetic protocols is of great importance for manufacture purposes, a fundamental requirement for an efficient translation of this technology into the clinic. The main objective of this PhD thesis is the study and fabrication of core-shell-type magnetic mesoporous silica nanoparticles (M-MSNs) for their application as theranostic nanodevices in the field of nanomedicine. A comprehensive study about the synthesis and characterization of this type of nanomaterials is presented with the aim of obtaining core-shell M-MSNs with well-defined physico-chemical properties in a robust and reproducible way. The fabrication of such particles would provide a versatile and reliable platform for the development of more complex nanodevices with advanced functionalities. The thesis has been structured into several chapters that are briefly summarized as follows: ¿Chapter 1 is an introduction to the topic of nanomedicine, highlighting the importance of nanoparticles in the development of new biomedical applications. Mesoporous silica nanoparticles are then introduced, showing the great versatility that this nanomaterials offer for the development of theranostic nanodevices and smart drug delivery systems. Finally, the development of nanodevices with well-defined physico-chemical properties is identified as a crucial requirement for overcoming biological barriers and facilitate the translation of nanomedicines from the bench to bedside. ¿Chapter 2 presents the aims of this thesis and the specific objectives that are addressed in the following chapters. ¿Chapter 3 is devoted to the synthesis and characterization of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs), which are later used as magnetic seeds for the synthesis of core-shell M-MSNs. USPIONs are prepared through a simple coprecipitation method using mild reaction conditions. The obtained nanoparticles are fully characterized and their magnetic properties are analyzed focusing on magnetic hyperthermia and dual MR imaging applications. ¿Chapter 4 is a comprehensive study about the preparation of monodisperse core-shell M-MSNs. The main concepts related to the synthesis and formation mechanisms of this type of nanomaterials are revised, together with the reaction parameters that are expected to have a major contribution on the reaction. As a starting point, a general synthetic protocol for the synthesis of core-shell M-MSNs is presented. Then, specific reaction parameters are investigated in order to understand their effect on the physico-chemical properties of the obtained nanoparticles. The application of a semi-empirical model to the optimization stage is presented in an attempt to provide an adequate reference framework to understand the formation of this complex nanodevices. ¿Chapter 5 presents a detailed analysis about the characterization of mesoporous silica materials and, in particular, the assessment of the mesoporous structure of MSNs with a radial distribution of wormhole-like channels. The effects that specific reaction parameters have on the mesoporous silica structure of core-shell M-MSNs are also analysed, providing additional information about the formation of this type of nanoparticles. ¿Chapter 6 gathers the main conclusions of this thesis.
Sánchez Cabezas, S. (2019). Development of a reproducible and optimized synthetic protocol for the preparation of monodisperse core-shell-type magnetic mesoporous silica nanoparticles [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/129878
TESIS
Cote, Shaun. "Characterization of the structure and function of NF-KappaB essential modulator and its interaction with inhibitor of KappaB Kinase Beta and development of a screening protocol to discover and validate inhibitors of the interaction." Thesis, 2014. https://hdl.handle.net/2144/14292.
Повний текст джерелаWang, Te-Kwei, and 王得貴. "Characterizations of Time Synchronization Protocols and Their Applications." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/34953571320982686534.
Повний текст джерела國立臺灣大學
電機工程學研究所
100
Time synchronization protocols play an important role in numerous applications, such as instrumentation, power systems and high-speed communications. With the rapid advances in technology, the demands placed on the accuracy of time synchronization have increased. The precision time synchronization protocol (PTP) was the first protocol developed to successfully synchronize distributed devices in the sub-microsecond (10-6 seconds) range. This dissertation firstly introduces the background knowledge regarding time synchronization protocols as well as the measurement of time and frequency. Then the ideas of PTP are investigated. New applications of PTP are proposed then. For example, PTP can be used as the case of the time measurement system to replace the Time Interval Counter (TIC). The benefits of PTP based time frequency measurement include low cost and high efficiency. The Gray Proportional Integral (GPI) controller and the Threshold Proportional Integral (TPI) controller are proposed to reduce the transient time interval during the time synchronization processes. Since PTP can provide time synchronization precision in the sub-microsecond (10-6 seconds) level, the time stamps are used as the encryption parameters. The pass words are revised every second. Such hind of Time Varying Encryption System (TVES) is quite safe since it is difficult to be cracked.
King, Daniel Allen. "Development of novel mass spectrometric protocols for rapid protein characterization." 2002. http://purl.galileo.usg.edu/uga%5Fetd/king%5Fdaniel%5Fa%5F200208%5Fphd.
Повний текст джерелаDirected by Ron Orlando. Includes articles submitted to Rapid communications in mass spectrometry, and Biochemistry. Includes bibliographical references.
Kim, Sohee. "Advanced characterization of crumb rubber modified asphalts using protocols developed for complex binders." 2000. http://catalog.hathitrust.org/api/volumes/oclc/47206813.html.
Повний текст джерелаTypescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 114-116).
Moore, Eve. "Developing Protocols to Facilitate the Enrichment and Characterization of Hydrocarbon-degrading Anaerobic Microbial Communities." Thesis, 2009. http://hdl.handle.net/1807/18927.
Повний текст джерелаMutavhatsindi, Hygon. "Characterization of PFF1010c, a type IV Plasmodium fasciparum heat shock protein 40." Diss., 2016. http://hdl.handle.net/11602/626.
Повний текст джерелаDepartment of Biochemistry
Malaria is caused by protozoa of the genus Plasmodium. Malaria accounts for approximately more than half a million deaths yearly. Of the five species of Plasmodium, P. falciparum accounts for the most deadly form of the disease. P. falciparum survives under various physiological conditions during its life cycle. The parasite employs its molecular chaperones machinery particularly heat shock proteins (Hsps) to protect its protein constituents during physiological stress. Hsps are conserved molecules that constitute a major part of the cell’s molecular chaperone system. P. falciparum Hsps play an important cyto-protective role guaranteeing that the malarial parasite survives under the severe conditions that prevail in the host environment. PFF1010c is a type IV P. falciparum heat shock protein 40. PFF1010c is predicted to be expressed only at the gametocyte stage of the malarial parasite’s life cycle. The aim of the current study was to investigate the expression PFF1010c by parasites and the gametocyte stage as well as characterize the structure-function features of the protein. PFF1010c was successfully expressed in E. coli cells. Despite successful expression of the protein, its purification proved problematic. The attempt to purify PFF1010c was carried out under both native and denaturing conditions. Far Western blot analysis to investigate direct interaction between PFF1010c and PfHsp70-1 was conducted and no interaction was observed. Malarial parasites were harvested at different stages and total protein was isolated. The expression of PFF1010c was confirmed to occur at the gametocyte stage of the parasite’s development using Western blot analysis. This study confirmed that PFF1010c is only expressed at the gametocyte stage of the malarial parasite. Furthermore, PFF1010c was not expressed at the asexual stage. Possible interactors of PFF1010c were predicted by STRING, a bioinformatics based tool. The expression of PfHsp90, PfHop and PfHsp70-1 at the gametocyte stage was investigated and confirmed by Western blot analyses.
Muthuswami, Rohini. "DNA-Dependent ATPase A : overexpression and characterization of the ATP hydrolyzing domain of the enzyme and identification of a novel class of inhibitors specific for this domain /." 1998. http://wwwlib.umi.com/dissertations/fullcit/9930126.
Повний текст джерелаPinto, Vanessa Patrícia Dias. "Comparison of Extracellular Vesicles isolation protocols from the plasma of patients with multiple myeloma: size characterization, expression of EV markers and microRNAs." Master's thesis, 2016. http://hdl.handle.net/10316/34192.
Повний текст джерелаMultiple myeloma (MM) is a plasma cell malignancy characterized by uncontrolled production of monoclonal immunoglobulins within the bone marrow. Despite the recent major treatment advances, obtained with the introduction of new therapeutic agents such as bortezomib and lenalidomide, drug resistance is a major obstacle to therapeutic success. Therefore, it is urgent to identify biomarkers of MM drug resistance. Extracellular vesicles (EVs) (including microvesicles and exosomes) are released by various cell types, contributing to intercellular communication. Tumor cells such as MM cells also release EVs, whose cargo may be transferred into recipient cells. Importantly, the presence of markers in the circulating EVs shed by tumor drug resistant cells, including particular microRNAs such as miR-21, may allow the detection of biomarkers of drug resistance. Therefore, this study aimed at selecting a method and optimizing a protocol for the isolation of EVs with different sizes (possibly exosomes and microvesicles) from plasma samples of MM patients. Thus, a comparison of methods was carried out, including ultracentrifugation, a commercial kit (ExoQuick) and qEV size exclusion chromatography (SEC) columns. The isolated EVs were characterized in terms of size and purity, by dynamic light scattering (DLS) and transmission electron microscopy (TEM), respectively. In addition, the analysis of EV markers such as Hsp70, CD63 and CD9 was also carried out (by Western Blot), together with the detection of possible cellular contaminants and protein contaminants from the plasma. Finally, the expression levels of miR-21 and miR-16 was attempted (by qRT-PCR). All the three methods allowed the isolation of EVs, but with distinct sizes. The ultracentrifugation allowed the isolation of EVs together with contaminants (possibly lipoproteins). In addition, this protocol had the disadvantage of requiring an expensive equipment (ultracentrifuge) and taking several hours. Moreover, the amount of total protein recovered from the ultracentrifugation protocol was very low. The ExoQuick kit allowed higher yields of total protein to be recovered from the isolated EVs and it was a much quicker protocol than the ultracentrifugation one; however, it only isolated the smaller vesicles (possibly exosomes). The qEV SEC columns were the preferred approach, since they enabled a rapid isolation of EVs with various sizes (possiblyexosomes and microvesicles). In addition, by pooling the various fractions collected, it is possible to have enough protein to be analysed by Western blot. Finally, preliminary results indicate that RNAse treatment is necessary when analysing miRs from EV samples. In addition, when pre-treating samples with RNase, the three protocols seem to allow detecting similar levels of miRs. However, these preliminary results need to be confirmed before conclusions can be taken.
O mieloma múltiplo é uma neoplasia maligna caracterizada pela produção descontrolada de imunoglobulinas monoclonais na medula óssea. Apesar dos recentes avanços nos tratamentos, obtidos sobretudo com a introdução de novos agentes terapêuticos como o bortezomib e a lenalidomida, a resistência aos fármacos continua a ser um dos maiores obstáculos para o sucesso terapêutico. Assim, é urgente identificar biomarcadores de resistência em mieloma múltiplo. Vesículas extracelulares (incluindo microvesículas e exossomas) são libertadas por vários tipos celulares, contribuindo para a comunicação intercelular. Células tumorais de mieloma múltiplo também libertam essas vesículas extracelulares, que transportam no seu interior componentes que podem ser transferidos parar outras células. A presença de marcadores nas vesículas extracelulares circulantes no sangue, libertadas por células tumorais resistentes a fármacos, nomeadamente microRNAs como o miR-21, podem permitir a detecção de biomarcadores de resistência. Assim, este projecto teve o objectivo de selecionar um método e optimizar um protocolo de extração de vesículas extracelulares com diferentes tamanhos (possivelmente exossomas e microvesículas) existentes no plasma de doentes com mieloma múltiplo. Para isso, foi realizada uma comparação entre métodos, incluindo a ultracentrifugação, um kit comercial (ExoQuick) e colunas de cromatografia de exclusão por tamanho (qEV). As vesículas extracelulares isoladas foram caracterizadas em termos de perfil de tamanho e pureza, respectivamente, por “Dynamic light scattering” (DLS) e por microscopia electrónica de transmissão (TEM). Além disso, a presença de marcadores como o Hsp70, CD63 e CD9 foi também analisada (por Western blot), bem como a presença de possíveis contaminantes celulares e proteicos com origem no plasma. Por fim, a expressão de miR-21 e miR-16 foi igualmente realizada (por qRT-PCR). Os três métodos permitiram a extração de vesículas extracelulares, mas com diferentes tamanhos. A ultracentrifugação permitiu isolar vesículas extracelulares mas contendo contaminantes (possivelmente lipoproteínas). Este protocolo tem também as desvantagens de requerer equipamento dispendioso (ultracentrífuga) e de ser muito moroso. Além disso, a quantidade total de proteína conseguida com este método foi muito reduzida. Por sua vez, ExoQuick permitiu precipitar grandes quantidades de proteína total a partir de amostras de vesículas extracelulares, tendo sido ainda um protocolo muito maisrápido em comparação com a ultracentrífugação; contudo, apenas permitiu isolar vesículas de pequenos tamanhos (possivelmente exossomas). As colunas de cromatografia qEV mostraram ser o protocolo preferido, uma vez que permitiram o rápido isolamento de vesículas extracelulares com diferentes tamanhos (possivelmente exossomas e microvesículas). Além disso, juntando as várias frações recolhidas das colunas qEV, é possível obter quantidade de proteína suficiente para ser analisada por Western blot. Por fim, resultados preliminares indicaram que o tratamento com RNase é necessário para a análise de miRs a partir de amostras de vesículas extracelulares. Além disso, com o pré-tratamento das amostras com RNase, os três protocolos parecem permitir detectar quantidades semelhantes de miRs. Contudo, estes resultados preliminares precisam ser confirmados para que se possam retirar conclusões.
cancro, mieloma múltiplo, resistência aos fármacos, vesículas extracelulares, miRs