Дисертації з теми "Proteomics approaches"
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Johnson, Hannah. "New Approaches to Quantitative Proteomics." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492739.
Повний текст джерелаJiang, Yanjie. "Approaches for Improved Positional Proteomics." ScholarWorks@UNO, 2013. http://scholarworks.uno.edu/td/1715.
Повний текст джерелаGetao, Shi. "DEVELOPMENT OF FUNCTIONAL PROTEOMICS APPROACHES FOR STUDYING RETROGRADE TRANSPORT." Phd thesis, Ecole Normale Supérieure de Paris - ENS Paris, 2011. http://tel.archives-ouvertes.fr/tel-00635924.
Повний текст джерелаBarberis, Elettra. "New non-invasive approaches for proteomics and metabolomics analyses." Doctoral thesis, Università del Piemonte Orientale, 2020. http://hdl.handle.net/11579/115041.
Повний текст джерелаYang, Xu. "Quantitative Approaches for Protein Differential Expression Analysis." Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/36172.
Повний текст джерелаMaster of Science
Gauthier, Daniel. "Design, development and application of new technological approaches in subcellular proteomics." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=18721.
Повний текст джерелаLe domaine de la protéomique subcellulaire vise à décrire et analyser toutes les protéines présentes dans un compartiment subcellulaire précis à un temps donné. Contrastant avec la protéomique des cellules ou d'organismes complets, l'analyse d'organelles individuelles a généré des protéomes plus simples desquels l'information biologique pertinente peut être plus facilement extraite. À ce jour, le complément de protéines de plusieurs structures subcellulaires, incluant la mitochondrie, le lysosome, le peroxysome, le phagosome et le noyau a été décrit. L'évolution rapide et la puissance de l'instrumentation disponible couplées au développement d'outils biochimiques et bioinformatiques permettent maintenant aux scientifiques de générer des modèles d'organelles complets basés sur les données générées par la protéomique. Ce domaine, cependant, fait toujours face à plusieurs défis. Parmi ceux-ci, on doit mentionner l'analyse des protéines associées à la membrane dont la taille et l'hydrophobicité compliquent l'extraction, la séparation subséquente et l'analyse par spectrométrie de masse. Une autre limitation émergeante en protéomique subcellulaire est l'obtention d'une préparation très pure d'organelles d'intérêt et ce, en quantité suffisante, qui dépend toujours de longues et laborieuses centrifugations basées sur la densité comme méthode de choix pour la fractionnement subcellulaire. Le travail présenté dans cette thèse décrit le développement de nouvelles méthodes en protéomique subcellulaire qui s'adressent aux défis mentionnés précédemment, et leur application à des modèles biologiques pertinents. Dans le chapitre 2, nous présentons l'élaboration et la mise au point d'une approche investigatrice non discriminatoire pour étudier les protéines de membrane. Basée essentiellement sur des procédures sans détergent et sans gel de séparation, cette stratégie a permis l'identification de centaines$
Wang, Linan. "Proteomic Based Approaches for Differentiating Tumor Subtypes." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1482248318956052.
Повний текст джерелаPancotti, A. "PROTEOMIC APPROACHES IN DRUGS AND BIOMARKERS DISCOVERY." Doctoral thesis, Università degli Studi di Milano, 2013. http://hdl.handle.net/2434/217538.
Повний текст джерелаLiao, Peter Lee Ming Liao. "Bioinformatics approaches to cancer biomarker discovery and characterization." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1525694252170957.
Повний текст джерелаStrbenac, Dario. "Novel Preprocessing Approaches for Omics Data Types and Their Performance Evaluation." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16007.
Повний текст джерелаKemmer, Danielle. "Genomics and bioinformatics approaches to functional gene annotation /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-636-0/.
Повний текст джерелаNavarro, Sanz Miriam. "Proteomic and metabolomic approaches to study diabetic retinopahty." Doctoral thesis, Universitat Rovira i Virgili, 2018. http://hdl.handle.net/10803/670958.
Повний текст джерелаEl objetivo general de esta tesis doctoral fue desarrollar, analizar y validar nuevas herramientas bioinformáticas que conviertan los datos crudos de metabolómica (adquiridos por espectrometría de masas) en conocimiento biológico con el fin de estudiar las alteraciones en el proteoma y metaboloma de células humanas del pigmento retinal expuestas a condiciones de hiperglucemia y / o hipoxia. ⁻ Objetivos metodológicos: (i) Hemos analizado las bases de datos espectrales de masas de metabolómica basada en LC / MS. (ii) Finalmente, hemos generado y mejorado la caracterización de los datos de metabolómica de LC / MS centrándonos en la anotación de MS1 y MS2. ⁻ Objetivos biológicos: (iii) Hemos propuesto un nuevo método que detecta y analiza los cambios en las redes de interacción proteína-proteína (PPI) por condiciones hiperglucemia y / o hipoxia. (iv) Hemos presentado un flujo de trabajo novedoso que es capaz de predecir y validar las alteraciones metabólicas debidas a condiciones hiperglucemia y / o hipoxia integrando a la vez los datos de expresión de proteínas en las redes metabólicas.
The general objective of this doctoral thesis was to develop, analyse and validate new bioinformatic tools for converting raw MS-based metabolomics data into biological knowledge, in order to study alterations in the proteome and metabolome of human retinal pigment epithelium cells exposed to hyperglycemic and/or hypoxic conditions. To reach this general objective, this thesis has been structured in two main blocks: ⁻ Methodological aims: (i) We have analysed mass spectral databases for LC/MS-based untargeted metabolomics. (ii) Finally, we have generated and improved the characterization of LC/MS metabolomics data focusing on MS1 and MS2 annotation. ⁻ Biological aims: (iii) We have proposed a novel method that detects and analyses changes in protein-protein interaction (PPI) networks by hyperglycemic and/or hypoxic conditions. (iv) We have presented a novel workflow which is able to predict and validate metabolite alterations due to hyperglycemic and/or hypoxic conditions integrating protein expression data in metabolic networks.
Jackson, David Henry. "Evaluation of proteomics-based approaches for the discovery of novel markers of ovarian cancer." Thesis, University of Leeds, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418750.
Повний текст джерелаBranson, Owen E. "Improved tag-count approaches for label-free quantitation of proteome differences in bottom-up proteomic experiments." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1471553685.
Повний текст джерелаWang, Ying. "Exploring action mechanisms of chemotherapeutic agents in human cancer cell lines by biochemical and proteomic approaches." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36633306.
Повний текст джерелаXie, Zhengzhi. "Investigation of Plant Specialized Metabolism (Secondary Metabolism) Using Metabolomic and Proteomic Approaches." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/195218.
Повний текст джерелаWang, Ying, and 王穎. "Exploring action mechanisms of chemotherapeutic agents in human cancercell lines by biochemical and proteomic approaches." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B36633306.
Повний текст джерелаVilagran, Martí Ingrid. "Freezability markers for boar sperm: new proteomic approaches." Doctoral thesis, Universitat de Girona, 2016. http://hdl.handle.net/10803/403431.
Повний текст джерелаLa criopreservació espermàtica és actualment la tècnica més eficient per emmagatzemar les mostres d’esperma de porc durant llargs períodes de temps. Però, no totes les ejaculacions presenten la mateixa congelabilitat, permetent classificar tant els porcs com les seves ejaculacions entre els/les de bona (GFE) i mala (PFE) congelabilitat. Així, aquesta Tesi pretén adquirir noves aproximacions en l’avaluació i predicció de la congelabilitat espermàtica, per evitar la criopreservació de les PFE. Mitjançant Western Blot, es va determinar si dues proteïnes (ODF1/HSPB10 i VDAC2) eren marcadores de congelabilitat. També, el proteoma espermàtic i el del plasma seminal de GFE i PFE va ser comparat mitjançant gels 2D-DIGE, per tal de cercar marcadors potencials de la congelabilitat espermática. Les conclusions són que la VDAC2, la ACRBP i la TPI en l’esperma fresc i la FN1 en el plasma seminal poden ser emprades per predir la resistència de les ejaculacions porcines a la criopreservació
Otto, Andreas Willy [Verfasser]. "Mass spectrometry based proteomics approaches unraveling dynamic processes in the entire bacterial cell / Andreas Otto." Greifswald : Universitätsbibliothek Greifswald, 2011. http://d-nb.info/1010268120/34.
Повний текст джерелаSiatkowski, Marcin [Verfasser]. "Approaches to the Analysis of Proteomics and Transcriptomics Data based on Statistical Methodology / Marcin Siatkowski." Greifswald : Universitätsbibliothek Greifswald, 2014. http://d-nb.info/1050274954/34.
Повний текст джерелаZhou, Cong. "Machine Learning Based Protein Identitification and Partial Granger Causality : Novel Bioinformatics Approaches for Proteomics Research." Thesis, University of Sussex, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505888.
Повний текст джерелаGan, Chee Sian. "Response of Synechocystis sp. PCC 6803 to photoperiod and phosphate alterations using functional proteomics approaches." Thesis, University of Sheffield, 2007. http://etheses.whiterose.ac.uk/14506/.
Повний текст джерелаLee, Mark N. "Genomic Approaches to Dissect Innate Immune Pathways." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10692.
Повний текст джерелаPotier, David N. "The development of mass spectrometry based approaches to monitor protease activity in biological fluids." Thesis, University of Manchester, 2012. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:185527.
Повний текст джерелаLee, Lai-sheung. "Characterization of arsenic transformed rat lung epithelial cells (TLECs) by biochemical and proteomic approaches." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43085283.
Повний текст джерелаSigdel, Tara. "A Search for Zn(II) Metallochaperones in E. coli, Proteomic and Genomic Approaches." Miami University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=miami1128394584.
Повний текст джерелаLee, Lai-sheung, and 李麗裳. "Characterization of arsenic transformed rat lung epithelial cells (TLECs) by biochemical and proteomic approaches." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43085283.
Повний текст джерелаIzquierdo, García Eduardo. "Chemical approaches to the study of the ceramide synthase activity." Doctoral thesis, Universitat de Barcelona, 2020. http://hdl.handle.net/10803/671426.
Повний текст джерелаLos esfingolípidos (SLs) son una de las principales categorías de lípidos presentes en los organismos eucariotas. Los SL no sólo son componentes estructurales esenciales de las membranas celulares, sino que también actúan como moléculas señalizadoras. Las ceramidas (Cer) son una tipología de SL que están formadas por una base esfingoide y una cadena de ácido graso de longitud variable unidos a través de un enlace amida. Las Cer participan como segundos mensajeros en procesos celulares como la apoptosis, la autofagia, la diferenciación celular y la senescencia. Las ceramida sintasas (CerS) son un grupo de enzimas del retículo endoplasmático que catalizan la N-acilación de bases esfingoides, como la esfingosina, utilizando acil-CoAs de distintas longitudes, para dar Cer. En los mamíferos se han descrito seis isoformas de la CerS y cada una de ellas tiene preferencia por un pequeño grupo de ácidos grasos de longitud de cadena definida, por lo que cada una produce perfiles de Cer característicos. En los últimos años se ha visto que la longitud de la cadena acilo de las Cer influye en sus propiedades biofísicas y en las cascadas de señalización en las que participan. Además, se sabe que ciertas Cer están involucradas en el desarrollo de distintas enfermedades como el cáncer, la diabetes, el Alzheimer o la esclerosis múltiple. En este sentido, el desarrollo de herramientas adecuadas para el estudio de la actividad de CerS es fundamental para descifrar los mecanismos moleculares a través de los cuáles actúan las Cer, siendo este el objetivo principal que se persiguió en la presente tesis doctoral. La primera parte de la tesis se centró en el desarrollo de un nuevo ensayo para determinar la actividad CerS por medio del fenómeno de FRET. Para ello, se diseñaron y sintetizaron una serie de sondas esfingoides derivadas de la espisulosina, una pequeña quimioteca de análogos de ácidos grasos “clicables” de distintas longitudes de cadena y una colección de reactivos fluorescentes marcados con un grupo biciclo[6.1.0]nonino (BCN) o 1,2,4,5-tetrazina (Tz). Las propiedades de absorción y emisión de fluorescencia de estos compuestos fueron estudiadas en varios disolventes a través de experimentos “en cubeta”. En base a estos experimentos anticipamos que las parejas de fluoróforos seleccionadas eran adecuadas para su uso en experimentos de FRET. A continuación, se evaluó la incorporación metabólica de las distintas sondas esfingoides y de los varios análogos de ácidos grasos en medios biológicos. Los estudios de lipidómica mostraron que tanto las sondas como los análogos de ácidos grasos eran procesados por las CerS para dar las Cer correspondientes. Sin embargo, los análogos de ácidos grasos también entraron en otras rutas metabólicas de los lípidos dando lugar a un elevado ruido de fondo tras la reacción de marcaje de fluorescencia. Nuestros intentos para solventar este problema fueron en vano y, por tanto, finalmente no fue posible la implementación del ensayo de fluorescencia para medir la actividad CerS. En la segunda parte de la tesis se propuso el desarrollo de nuevos CLIPTACs dirigidos a la degradación de CerS, como alternativa a los inhibidores clásicos para la modulación de la actividad CerS. Para ello se diseñaron y sintetizaron cuatro derivados de BCN que contuvieran un ligando para reclutar distintos enzimas E3 ligasas de ubiquitina. Estos reclutadores de E3 ligasa serán utilizados en un futuro en combinación con un derivado de la Jaspina B, un análogo del sustrato de las CerS, para obtener los CLIPTACs deseados.
Neuhauser, Nadin [Verfasser], Dimitrij Akademischer Betreuer] Frischmann, and Matthias [Akademischer Betreuer] [Mann. "Computational approaches to enhance mass spectrometry-based proteomics / Nadin Neuhauser. Gutachter: Dimitrij Frischmann ; Matthias Mann. Betreuer: Dimitrij Frischmann." München : Universitätsbibliothek der TU München, 2013. http://d-nb.info/1035274531/34.
Повний текст джерелаFilip, Szymon [Verfasser]. "Identification of protein biomarkers for chronic kidney disease progression using state-of-the-art proteomics approaches / Szymon Filip." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2016. http://d-nb.info/1119803438/34.
Повний текст джерелаCLAUDIA, BOLDRINI. "Proteomics approaches to investigate the role of lysine acetylation in type 2 diabetes mellitus and in breast cancer." Doctoral thesis, Università di Siena, 2018. http://hdl.handle.net/11365/1046537.
Повний текст джерелаPatel, Vishal N. "Colon Cancer and its Molecular Subsystems: Network Approaches to Dissecting Driver Gene Biology." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1310087563.
Повний текст джерелаAbdullah, Sewa Faraj. "Proteomics approaches to polyketide synthases interfaces by mass spectrometry and NMR spectroscopy and the application of chemometrics to metabolomics." Thesis, University of Bristol, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.682232.
Повний текст джерелаSakalauskaite, Jorune. "Proteomic approaches for archaeology and cultural heritage : characterising ancient proteins preserved in mollusc shells." Thesis, Bourgogne Franche-Comté, 2021. http://www.theses.fr/2021UBFCK003.
Повний текст джерелаArchaeological mollusc shell artifacts and ecofacts are valuable sources to study past cultures and provide insights on how people exploited their environments. Mollusc shells were often used as raw materials to make personal ornaments and are abundantly found in archaeological sites. However, minute, heavily worked and/or fragmented shell ornaments are rarely identified at different taxonomic levels, due to limited availability of analytical approaches to determine taxon-specific (diagnostic) features. In recent years, proteomics, which exploits the high sensitivity of modern mass spectrometry techniques, has been successfully applied to the study of a variety of bioarchaeological remains, opening a new research field referred to as ‘palaeoproteomics’. While modern mollusc shells represent key study models in biomineralization research to investigate the molecular mechanisms of biocalcification and mineral deposition, palaeoproteomics on archaeological shells has never been carried out before. The challenges are mainly due to the intrinsic physico-chemical features of their substrates (including low protein content), and the paucity of protein sequences for reference.Therefore, this work presents a comprehensive proteomic-based investigation of archaeological mollusc shells, grounded in biomineralization research, and the first application of palaeoproteomics to shells. The main objective of this PhD was to investigate three key concepts: whether shell proteins carry taxonomic information, could be preserved over archaeological times scales and could be used as molecular barcodes for the taxonomic determination of ancient shell artifacts.High performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was used for an in-depth proteomics characterisation of a selected model, Spondylus gaederopus, demonstrating that its shell-associated proteins are very different from other models and likely represent lineage-specific sequences. Peptide mass fingerprinting (PMF) by MALDI-TOF mass spectrometry was employed to obtain sequence information from thirty-four different molluscan taxa. Intracrystalline shell proteins displayed clearly distinct PMFs indicating that they may encode taxonomic information and could be used as molecular barcodes to identify archaeological mollusc shells. Accelerated aging experiments were performed to mimic the diagenesis of Spondylus shell and the stability of intracrystalline shell proteins was investigated by a combination of immunochemistry and quantitative TMT proteomics. We concluded that Spondylus represents a favourable system for protein preservation, as evidenced by the thermal stability of shell peptide sequences. However, the observed degradation patterns pinpointed the complexity of the whole diagenesis process, which does not entirely follow expected trajectories from theoretical models.Finally, a set of archaeological shell ornaments, recovered from different Mesolithic and Neolithic European prehistoric sites, were studied by proteomics coupled with structural, biomolecular and geochemical analyses. The studies showed that PMF by MALDI-TOF approach was not able to characterise these archaeological samples, which are very degraded or and/or have low protein contents, and the analysis of ancient shell proteins requires higher sensitivity, as offered by HPLC-MS/MS mass spectrometry. Indeed, palaeoproteomic analysis by HPLC-MS/MS of small “double-button” ornaments identified that they were made of freshwater mother-of-pearl shells, resolving the long-standing debate over their biological origin and provenance. The research carried out in this thesis highlights the immense potential of proteomics-based approaches to study archaeological and palaeontological shell remains
Amorim, Ines Da Silva. "Investigating novel therapeutic approaches and targets to prevent synapse degeneration." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/23550.
Повний текст джерелаShirsat, Siddhita Abhijeet Shirsat. "Identification of Proteins from Lanthionine Ketimine Ethyl Ester (LKE)- treated and untreated Rat Glioma 2 (RG2) Cells using Proteomic Approaches." University of Toledo / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1469272613.
Повний текст джерелаRudolph, Jan Daniel [Verfasser], and Matthias [Akademischer Betreuer] Mann. "Development and application of software and algorithms for network approaches to proteomics data analysis / Jan Daniel Rudolph ; Betreuer: Matthias Mann." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1206877723/34.
Повний текст джерелаMadden, Robert T. "Biochemical and Proteomic Approaches to Determine the Impact Level of Each Step of the Supply Chain on Tomato Fruit Quality." Scholar Commons, 2019. https://scholarcommons.usf.edu/etd/7853.
Повний текст джерелаGuo, Jingshu. "Structural Analysis of Macromolecular Complexes Using Electrospray Ionization Mass Spectrometry Based Approaches." University of Toledo / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1373036513.
Повний текст джерелаNukala, Sarath Babu. "Bioanalytical and proteomic approaches in the study of pathologic ECs dysfunctionality, oxidative stress and the effect of PFKFB3 modulators." Doctoral thesis, Universitat de Barcelona, 2019. http://hdl.handle.net/10803/671200.
Повний текст джерелаStella, Roberto. "Skeletal muscle analysis by two different approaches: - an in vivo model to study the physiology of cellular prion protein - proteomics to identify biomarkers of illicit animal treatments." Doctoral thesis, Università degli studi di Padova, 2011. http://hdl.handle.net/11577/3422863.
Повний текст джерелаI dati riportati nella presente tesi sono stati ottenuti in due diversi progetti. Pertanto, la tesi è divisa in due distinti capitoli. Il primo capitolo, che si riferisce agli articoli I e II, riporta uno studio volto a chiarire il ruolo fisiologico della proteina prionica cellulare (PrPC) utilizzando un modello in vivo di rigenerazione del muscolo scheletrico. Il secondo capitolo si riferisce all'articolo III, in cui si è cercato di individuare possibili marcatori biologici di trattamento illecito di vitelloni con agenti promotori della crescita (GPA), utilizzando un approccio di elettroforesi bidimensionale (2DE), in combinazione con spettrometria di massa. Focalizzando l'attenzione sul rapporto tra PrPC e la rigenerazione del muscolo scheletrico in un modello in vivo, nella prima linea di ricerca (Capitolo I) abbiamo indagato se e come la proteina influenza la proliferazione e la differenziazione delle cellule precursori del muscolo. PrPC è una glicoproteina ancorata alla membrana esterna delle cellule coinvolta nella comparsa di malattie neurodegenerative rare e mortali, conosciute con il nome di encefalopatie spongiformi trasmissibili (EST) o malattie da prioni. L'evento alla base delle EST è la conversione della PrPC in una isoforma con una modificata conformazione che dà origine al prione, un agente infettivo neurotossico. Anche se ora sono disponibili molte informazioni sulle diverse vie di infezione da parte del prione, sia i meccanismi alla base della neurotossicità, sia il ruolo fisiologico della PrPC rimangono poco chiari. Tuttavia, l'uso di diversi modelli animali e cellulari ha suggerito molteplici funzioni putative per la PrPC, che vanno dalla protezione cellulare contro lo stress ossidativo e stimoli apoptotici, all'adesione, proliferazione e differenziazione cellulare. Il muscolo scheletrico esprime quantità significative di PrPC, e molti studi l'hanno correlato alla fisiopatologia della proteina. Pertanto, al fine di chiarire il ruolo fisiologico della PrPC in questo tessuto, abbiamo impiegato una protocollo di degenerazione/rigenerazione del muscolo tibiale anteriore, che ci ha permesso di confrontare il processo rigenerativo in topi che esprimono, o meno, PrPC. I parametri istologici e biochimici analizzati hanno fornito prove della rilevanza fisiologica della PrPC e del suo coinvolgimento negli eventi di segnalazione coinvolti nella rigenerazione muscolare. Infatti, è stato osservato che l'assenza della PrPC ritarda significativamente il processo di rigenerazione rispetto ai muscoli WT. In particolare, abbiamo trovato che la mancanza di PrPC causa un'attenuazione della via di segnalazione attivata dal TNF-alpha, che porta ad una ridotta attivazione della chinasi p38, e - conseguentemente - ritarda l'uscita dal ciclo cellulare e la differenziazione dei precursori miogenici. È importante sottolineare che il ripristino dell'espressione della PrPC abolisce completamente il fenotipo osservato nei muscoli di topi PrP-KO, sottolineando che la regolazione delle vie di segnalazione da parte PrPC ha una chiara importanza fisiologica anche in tessuti extraneuronali. La seconda linea di ricerca, descritta nel capitolo II, è stata volta a creare una strategia basata su tecniche di proteomica per l'identificazione di trattamenti farmacologici illeciti in vitelloni. L'approccio classico per la rilevazione di questa pratica illegale non è adatto ad individuare composti sia di struttura chimica sconosciuta, sia di farmaci presenti a livelli inferiori alla soglia di quantificazione delle tecniche analitiche attualmente impiegate. Il successo delle analisi istologiche di organi bersaglio, che vengono indirettamente modificati a seguito di questi trattamenti, ha suggerito che gli approcci basati sulla ricerca degli effetti biologici delle molecole in esame, piuttosto che sulla rilevazione diretta dei loro residui, potrebbero essere molto utili. Il vantaggio più rilevante di questa metodologia è che le modificazioni del tessuto indotte da un trattamento farmacologico rimangono evidenti molto tempo dopo la fine dei trattamenti illeciti, quando i residui chimici non sono più, o quasi, rilevabili. D'altra parte, questo approccio è notevolmente limitato dalla capacità di valutazione dei tecnici e l'analisi e influenzata dalla soggettività. Per questo, sono necessarie nuove strategie per il rilevamento dei biomarcatori indiretti presenti nei fluidi animali o nei tessuti. Questi biomarcatori possono essere molecole naturalmente presenti, come ad esempio proteine che abbiano subito modifiche nella struttura, o nella concentrazione, a seguito di variazioni della condizione fisiologica dell'animale. Per identificare tali marcatori biologici di trattamenti farmacologici illeciti nei bovini da carne, abbiamo adottato un approccio proteomico, mediante elettroforesi differenziale su gel in due dimensioni (2D-DIGE) e analisi in spettrometria di massa, al fine di confrontare i pattern di espressione proteica di muscolo scheletrico tra animali trattati farmacologicamente e di controllo. A questo scopo, i vitelloni appartenenti al gruppo di trattamento sono stati sottoposti a tre differenti protocolli farmacologici, mediante l'impiego di agenti promotori della crescita. Due di questi trattamenti hanno portato ad un notevole effetto anabolico rispetto agli animali non trattati, mostrando di conseguenza un'alterazione del proteoma del muscolo scheletrico. Le mappe proteiche dei campioni appartenenti ai gruppi di trattamento e di controllo sono state confrontate utilizzando il software DeCyder per analisi di dati derivanti da 2D-DIGE. Si è poi cercato di identificare, con un approccio di spettrometria di massa (MALDI) in tandem (MS/MS), tutte le proteine che mostrano una significativa alterazione nei loro livelli di espressione in seguito a somministrazione di agenti promotori della crescita. Tra le 169 proteine che cambiano in espressione in seguito al trattamento farmacologico, sono state identificate 29 proteine diverse, la maggior parte delle quali è coinvolta nella contrazione muscolare e nel metabolismo energetico. Questi risultati confermano i precedenti risultati sul meccanismo d'azione degli agenti promotori della crescita, e possono essere utili per sviluppare nuove strategie per l'identificazione di trattamenti farmacologici illeciti nei bovini da carne.
Stahl, Antje [Verfasser], Matthias [Akademischer Betreuer] [Gutachter] Ullrich, Frank Oliver [Gutachter] Glöckner, and Jens [Gutachter] Harder. "Interaction of the Marine Bacterium Marinobacter adhaerens HP15 with the Diatom Thalassiosira weissflogii Analyzed by Proteomics Approaches / Antje Stahl ; Gutachter: Matthias Ullrich, Frank Oliver Glöckner, Jens Harder ; Betreuer: Matthias Ullrich." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2016. http://d-nb.info/1113875593/34.
Повний текст джерелаValdeolivas, Urbelz Alberto. "Approaches to explore multiplex biological networks and application to study premature aging diseases." Thesis, Aix-Marseille, 2019. http://www.theses.fr/2019AIXM0106.
Повний текст джерелаGenes and proteins do not act isolated in cells but rather interact to perform their functions in signaling pathways, molecular complexes, or, more generally, biological processes. These interactions can be represented as large networks in which nodes are genes or proteins and edges represent their interactions. Various graph-theory based approaches have been developed to extract the functional knowledge contained in biological networks. Nevertheless, these methods have been mainly applied to individual networks, ignoring the diversity of biological interactions. We state here that these different types of interactions can be represented as multiplex networks, i.e. collections of networks sharing the same nodes, leading to a more accurate description of biological systems. This thesis focuses on the extension from individual to multiplex networks of some of the state-of-the-art guilt-by-association methods in computational biology, and on their application to the study of human diseases. On the application side, we concentrate on premature aging diseases, a group of rare genetic disorders that resemble some aspects of physiological aging at an early age. In this framework, we applied our algorithms to detect the modules associated to more than 70 disorders annotated with at least one premature aging related phenotype. The results revealed the landscape of perturbed molecular processes in premature aging diseases, which can be paralleled with the hallmarks of physiological aging to help identifying common and specific features
Heilmann, Geronimo [Verfasser], and Markus [Akademischer Betreuer] Kaiser. "The use of mass-spectrometry-based chemical proteomics approaches in bioactivity annotation of natural products and biocatalyst discovery : Applications to the natural product Zelkovamycin and fungi / Geronimo Heilmann ; Betreuer: Markus Kaiser." Duisburg, 2020. http://d-nb.info/1221960318/34.
Повний текст джерелаASTORI, EMANUELA. "IN VITRO AND IN VIVO APPROACHES TO STUDY OXIDATIVE STRESS, ANEMIA AND DYSBIOSIS IN CHRONIC KIDNEY DISEASE." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/818976.
Повний текст джерелаJufvas, Åsa. "Human Adipocytes : Proteomic Approaches." Doctoral thesis, Linköpings universitet, Avdelningen för cellbiologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-125907.
Повний текст джерелаRICCI, SARA. "Study of biotic and abiotic stresses in Solanaceae by metabolic and proteomic approaches." Doctoral thesis, Università di Foggia, 2017. http://hdl.handle.net/11369/363315.
Повний текст джерелаTan, Kit-Yee. "The characterisation of RKIP using proteomic approaches." Thesis, University of Glasgow, 2011. http://theses.gla.ac.uk/2569/.
Повний текст джерелаFranco, Catarina de Matos Ferraz. "Proteomics based approach to understand tissue regeneration." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2011. http://hdl.handle.net/10362/14118.
Повний текст джерелаMost echinoderm species share an outstanding capacity for regeneration that is maintained throughout the adult animal lifespan. Regeneration allows these deuterostomes to recover from predation injuries or selfinduced arm autotomy, which are known to occur frequently in nature. Although echinoderms are extremely interesting in terms of their phylogenetic proximity to chordates, most areas of echinoderm research have been neglected in recent years. These wonderful animals quickly shifted from being the preferred animal models in the 19th-20th centuries of the pioneer regenerationists to scientific oblivion. Other species, for which the possibility of conducting genetic studies became available, are now favored. After the sequencing of an echinoderm species genome, the sea urchin Strongylocentrotus purpuratus in 2006, several scientific reports of interesting molecular studies were published.(...)
Fundação para a Ciência e a Tecnologia
Vitorino, Rui Miguel Pinheiro. "Dental caries: a proteomic approach." Doctoral thesis, Universidade de Aveiro, 2004. http://hdl.handle.net/10773/17671.
Повний текст джерелаA cárie dentária é uma doença complexa que afecta uma grande parte da população mundial independentemente do sexo, idade ou etnia. Este processo é dependente de factores biológicos que se encontram presentes na saliva e placa dentária. Em seguimento do referido, amostras de saliva foram colectadas de indivíduos caracterizados em função dos índices DMFT e DMFS. A avaliação dos convencionais parâmetros clínicos como por exemplo fluxo salivar, capacidade tampão, pH usados na avaliação do risco para a cárie dentária em combinação com dieta, hábitos de higiene e tabagismo foram realizados para todos os indivíduos participantes do qual se observou a ausência de uma positiva correlação com o índice DMFT. Uma vez que os factores biológicos presentes na saliva influenciam o processo da cárie dentária, o objectivo deste trabalho consistiu na investigação de uma possível correlação entre as proteínas e peptídeos da saliva e o processo da cárie dentária. A caracterização das proteínas e peptídeos da saliva foi alcançada utilizando electroforese bidimensional (2-DE), cromatografia líquida de alta resolução (HPLC) combinada com a espectrometria de massa (MS), do qual resultou a identificação de 38 proteínas das quais 12 foram identificadas pela primeira vez por 2-DE e 22 peptídeos por HPLC-MS também identificados pela primeira vez. Ensaios realizados para o estudo da composição da película dentária seguiram a mesma metodologia descrita para a caracterização das proteínas e peptídeos da saliva sendo realizados inicialmente in vitro e confi rmados posteriormente por ensaios in vivo. A adsorção dos componentes salivares à hidroxiapatite é um processo selectivo com predominância de componentes salivares de baixo peso molecular. Contudo, amilase, lactoferrina, IgA salivar e anidrase carbónica VI foram também identificadas. A extracção sequencial usando guanidina e ácido trifluoroacético das proteínas/peptídeos adsorvidas à hidroxiapatite permitiu uma avaliação da força das ligações estabelecidas. Destes ensaios verificou-se que proteínas ricas em prolina (PRP-1/3), cistatina S, statherina e histatina 1 estabeleciam interacções fortes com a hidroxiapatite permanecendo adsorvidas após extracção com guanidina. As proteínas caracterizadas da saliva e da película dentária foram correlacionadas com o índice DMFT apresentando uma predominância de elevadas quantidades de cistatinas, PRP -1/3, statherina e histatina 1 no grupo de indivíduos sem cárie. O reduzido número de fragmentos em associação com as elevadas quantidades de cistatinas podem sugerir um controle mais eficiente da actividade proteólitica evitando desta maneira a degradação de importantes proteínas salivares no grupo de indivíduos sem cárie. A composição da película dentária é afectada pela composição proteica da saliva encontrando-se as referidas proteínas em maior quantidade. Os dados obtidos sugerem uma eficiente protecção por parte das proteínas da saliva contra a cárie dentária em particular a PRP-1/3, statherina e histatina 1, provavelmente devido à sua participação nos processos de remineralização na superfície do dente, e das cistatinas na diminuição da actividade proteólitica.
Dental caries is a complex disease process that affects a large proportion of the world population, regardless of gender, age and ethnicity. This process is dependent upon biological factors that are present within saliva and dental plaque. Following this, whole saliva was collected from selected individuals characterised according its DMFT and DMFS scores. Evaluation of the conventional clinical parameters such as flow rate, buffering capacity, pH used for caries risk assessment in combination with diet, hygiene and smoke habits was performed for all participating subjects showing absence of a statistic positive correlation with DMFT index. Since biological factors present on saliva influence dental caries process, the aim of this study was to investigate how salivary proteins and peptides are correlated with this pathology. Characterisation of salivary proteins and peptides was achieved using twodimensional gel electrophoresis (2-DE) and high performance liquid chromatography (HPLC) in combination with mass spectrometry (MS) resulting in the identification of 38 proteins, being 12 proteins identified by 2-DE and 22 peptides by HPLC-MS were identified for the first time. Experiments to study enamel pellicle composition were performed following the same methodology described for salivary proteins and peptides, initially in vitro being supported with in vivo assays. Adsorption of salivary components to hydroxyapatite showed to be a selective process with a predominance of low molecular weight salivary components. However, amylase, lactoferrin, S-IgA, carbonic anhydrase VI were also identified. A sequential extraction, using of guanidine and trifluoroacetic acid, of the adsorbed proteins/peptides to hydroxyapatite allowed to evaluate the strength of the establish interactions. From this experiments, proline-rich proteins (PRP -1/3), cystatin S, statherin, histatin 1 exhibited a strong interaction with hydroxyapatite remaining adsorbed after guanidine extraction. Characterised salivary proteins from whole saliva and enamel pellicle were correlated with DMFT index showing a predominance of higher amounts of cystatins, PRP-1/3, statherin and histatin 1 in caries free group. Decreased number of fragments in association with higher amounts of cystatins may suggest a more effective control in proteolytic activity which avoid the degradation of important salivary proteins from caries free group. Acquired pellicle composition is affected by whole saliva protein composition being the above referred proteins present in higher amounts. Obtained data suggest an effective protective role of several salivary proteins to dental caries in particular of PRP-1/3, statherin and histatin 1, possibly due to their participation on remineralization processes at the tooth surface, and of cystatins probably by decreasing proteolytic activity.