Дисертації з теми "Proteomics and metabolomics"
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Shiryaeva, Liudmila. "Proteomics and metabolomics in biological and medical applications." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-43520.
Повний текст джерелаSalji, Mark J. "Quantitative proteomics and metabolomics of castration resistant prostate cancer." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/30941/.
Повний текст джерелаHenneges, Carsten [Verfasser]. "Feature Selection and Data Mining for Proteomics and Metabolomics / Carsten Henneges." München : Verlag Dr. Hut, 2011. http://d-nb.info/1017353573/34.
Повний текст джерелаMörén, Lina. "Metabolomics and proteomics studies of brain tumors : a chemometric bioinformatics approach." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-111309.
Повний текст джерелаThomas, Funmilola Clara. "Acute phase proteins, proteomics and metabolomics in the diagnosis of bovine mastitis." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/6360/.
Повний текст джерелаWibom, Carl. "Multivariate analyses of proteomic and metabolomic patterns in brain tumors." Doctoral thesis, Umeå universitet, Onkologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-25670.
Повний текст джерелаHollywood, Katherine. "The development of bio-analytical techniques for the treatment of psoriasis and related skin disorders." Thesis, University of Manchester, 2010. https://www.research.manchester.ac.uk/portal/en/theses/the-development-of-bioanalytical-techniques-for-the-treatment-of-psoriasis-and-related-skin-disorders(eadbfad8-6ab0-4029-b618-a1a6d715c3af).html.
Повний текст джерелаPervukhin, Anton. "Molecular formula identification using high resolution mass spectrometry algorithms and applications in metabolomics and proteomics /." kostenfrei, 2009. http://d-nb.info/1001408578/34.
Повний текст джерелаMußotter, Franz [Verfasser]. "Application of proteomics and metabolomics in molecular investigations of sensitization to contact allergens / Franz Mußotter." Berlin : Freie Universität Berlin, 2018. http://d-nb.info/1159900582/34.
Повний текст джерелаHadrévi, Jenny. "Applying proteomics and metabolomics for studying human skeletal muscle with a focus on chronic trapezius myalgia." Doctoral thesis, Umeå universitet, Institutionen för integrativ medicinsk biologi (IMB), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-61399.
Повний текст джерелаAgana, Bernice A. "Mass Spectrometry-Based Proteomics and Metabolomics: Understanding Protein Interactions, Proteome Complexity and Perturbations in Cellular Metabolism." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1574636665012436.
Повний текст джерелаHappyana, Nizar [Verfasser], Oliver [Akademischer Betreuer] Kayser, and Robert [Gutachter] Verpoorte. "Metabolomics, proteomics, and transcriptomics of Cannabis sativa L. trichomes / Nizar Happyana. Betreuer: Oliver Kayser. Gutachter: Robert Verpoorte." Dortmund : Universitätsbibliothek Dortmund, 2014. http://d-nb.info/1101476958/34.
Повний текст джерелаGunnaiah, Raghavendra. "Functional characterization of wheat, fusarium head blight resistance (QTL) «Fhb1» based on non-target metabolomics and proteomics." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119639.
Повний текст джерелаLa fusariose de l'épi est une maladie fongique attaquant le blé (Triticum aestivum) induite par Fusarium graminearum. La fusariose cause de sévères pertes économiques dues à la réduction de la qualité et des rendements suite à la contamination par les mycotoxines trichothecene. La résistance du blé face à la fusariose est un trait quantitatif. Plus de 100 LCQ on été identifiés et un petit nombre a été validé. Cependant, les mécanismes de résistance gouvernés par ces LCQ sont peu connus. Fhb1 est le LCQ le plus consistent qui produit le plus grand effet face à la fusariose du blé. Une caractérisation fonctionnelle à l'aide d'un LC-MS à haute résolution de lignées isogéniques avec ou sans l'allèle susceptible Fhb1 a générée des profils de métabolites non ciblés ainsi que protéomique. Le Fhb1 d'un cultivar modérément résistant, Nyubai, a été associé avec le développement de la paroi cellulaire plus épaisse, surtout au niveau du rachis due à la déposition d'acides amides hydroxycinnamic (HCAAs), de glucosides phénolique ainsi que de flavonoïdes. Le gène codant pour une protéine hypothétique (GenBank: CBH32656.1) située près du locus Fhb1 a été identifiée comme étant possiblement une hydroxycinnamoyle transférase. Cette protéine déclencherait la biosynthèse de HCAAs. L'accumulation de DON a été plus élevée dans les deux lignes isogéniques. La détoxification de DON est un mécanisme associé avec Fhb1 (Chapitre III). Pour confirmer, l'allèle Fhb1 la résistance du cultivar Sumai-3 a été profilé. Contrairement aux lignes iso géniques, aucune présence constitutive de glycérophospholipides, n'a été détectée chez les lignées susceptibles en raison de la dégradation des membranes. La dégradation de membrane s'est avérée être causée par la mort cellulaire programmée comme le démontre le patron de dégradation de l'ADN de la variété susceptible NIL. Le locus TAA_ctg0954b.00390.1 fut identifié comme candidat pour le gène Fhb1 qui contient un domaine de liaison à la calmoduline et deux signaux de localisation nucléaire. Ce dernier fut donc annoté en tant que protéine de liaison à la calmoduline (TaCaMBP_Fhb1). TaCaMBP_Fhb1 est induit suite à l'infection du pathogène pour ensuite se lier à la calmoduline liée au Ca2+ pour ensuite initier une cascade de signaux qui inclut l'activation transcriptionnelle d'endonucléases qui clivent l'ADN génomique causant ainsi la mort cellulaire programmée. L'allèle résistante de TaCaMBP_Fhb1 présente une délétion au niveau du promoteur ce qui la rend non fonctionnel pour l'activation du signalement Ca2+ impliqué dans la mort cellulaire programmée. Le pathogène nécrotrophe F. graminearum se nourrit des tissus morts, se multiplie et produit plus de DON pour faciliter l'infection; perpétuant ainsi un cercle vicieux chez le génotype susceptible (Chapitre IV). C'est résultats on été confirmés à l'aide d'un profilage métabolique des rachis de la lignée résistante Sumai-3 et la lignée susceptible Roblin lors de l'infection avec (Wild : FgTri5+) trichothécène producteur et (Mutant :FgTri5- ) trichothécène non producteur qui sont deux isolats de F. graminearum. L'isolat producteur est parveu à inhiber plusieurs mécanismes de résistance de l'hôte dans les deux cultivars grâce à la production de DON. La résistance FHB à l'infection dans Sumai-3 était principalement lié à l'augmentation des parois cellulaires particulièrement au niveau des rachis à cause de la déposition de lignine, HCAAs et de flavonoïdes et partiellement due à l'augmentation des métabolite RR qui réduisent la biomasses des champignons ainsi que la synthèse des toxines. La résistance n'a pas été attribuée à détoxification de DON par l'UDP-glycosyltransferase, puisque les résultats étaient similaires dans les deux cultivars (Chapitre V). Les allèles résistants, des deux gènes candidats Fhb1 identifiés dans cette étude, pourraient-être ajoutés au génome de cultivars élites de blé pour augmenter leur résistance au FHB.
Freeman, Oliver. "The pathogenesis of diabetic neuropathy : a proteomic, metabolomic and electrophysiological investigation." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/the-pathogenesis-of-diabetic-neuropathy-a-proteomic-metabolomic-and-electrophysiological-investigation(a3caaa8a-9ff7-45c7-b6b2-66c8bb6aebf1).html.
Повний текст джерелаFraige, Karina. "Estudo comparativo do perfil metabolômico e proteômico de uvas (Vitis vinifera) durante o processo de maturação utilizando ferramentas bioanalíticas." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-25072012-091056/.
Повний текст джерелаAnalysis of the chemical composition of grapes is very important to evaluate harvest time and production of high quality wines. The study of grape metabolism is essential to define in which stages metabolites are produced, as well as proteins and genes that regulate these processes. Sugars, polyphenols and organic acids are important classes of metabolites related with grape development. Sugars are compounds that primarily indicate harvest time, and they are key substances in various biological processes. Polyphenols have been noted for its antioxidant activity, also for taking part in mechanisms of plant defense. Organic acids are responsible for organoleptic quality, and they are evolved in diverse metabolic processes. Proteins do not contribute significantly to the nutritional value, but they are important variety markers to verify adulterations of wines. Rio Grande do Sul is responsible for most of the grapes produced in Brazil, and recently Vale do São Francisco has received attention because of the production of these fruits. In some regions of Southeast a different pruning handle has started to obtain grapes with good levels of ripeness and resistance in developing fungal diseases. Grapes cultured in Água Vermelha and Louveira, São Paulo State, were studied during ripening in relation to physical-chemical analysis, proteomic profile, by two-dimensional electrophoresis and mass spectrometry; the metabolomic profile of anthocyanins, non-anthocyanin polyphenols, organic acids and sugars by chromatographic and electrophoretic techniques coupled to diode array and/or mass spectrometry detectors. The results were analyzed by multivariate analysis and compared with those of mature grapes from South and Northeast regions. The data show clustering of green grapes due to higher acidity and clusters of mature grapes due to higher anthocyanin and sugars concentrations, and the profile of anthocyanins can be used as a varietal marker. Considering the proteomic profile, it was possible to group different varieties of grapes with a trend in relation to geographical origin being also observed. It was identified 74 proteins related, mainly, to defense and stress response, carbohydrate metabolism and energetic metabolism.
Huang, Zhengyan. "Differential Abundance and Clustering Analysis with Empirical Bayes Shrinkage Estimation of Variance (DASEV) for Proteomics and Metabolomics Data." UKnowledge, 2019. https://uknowledge.uky.edu/epb_etds/24.
Повний текст джерелаNavarro, Sanz Miriam. "Proteomic and metabolomic approaches to study diabetic retinopahty." Doctoral thesis, Universitat Rovira i Virgili, 2018. http://hdl.handle.net/10803/670958.
Повний текст джерелаEl objetivo general de esta tesis doctoral fue desarrollar, analizar y validar nuevas herramientas bioinformáticas que conviertan los datos crudos de metabolómica (adquiridos por espectrometría de masas) en conocimiento biológico con el fin de estudiar las alteraciones en el proteoma y metaboloma de células humanas del pigmento retinal expuestas a condiciones de hiperglucemia y / o hipoxia. ⁻ Objetivos metodológicos: (i) Hemos analizado las bases de datos espectrales de masas de metabolómica basada en LC / MS. (ii) Finalmente, hemos generado y mejorado la caracterización de los datos de metabolómica de LC / MS centrándonos en la anotación de MS1 y MS2. ⁻ Objetivos biológicos: (iii) Hemos propuesto un nuevo método que detecta y analiza los cambios en las redes de interacción proteína-proteína (PPI) por condiciones hiperglucemia y / o hipoxia. (iv) Hemos presentado un flujo de trabajo novedoso que es capaz de predecir y validar las alteraciones metabólicas debidas a condiciones hiperglucemia y / o hipoxia integrando a la vez los datos de expresión de proteínas en las redes metabólicas.
The general objective of this doctoral thesis was to develop, analyse and validate new bioinformatic tools for converting raw MS-based metabolomics data into biological knowledge, in order to study alterations in the proteome and metabolome of human retinal pigment epithelium cells exposed to hyperglycemic and/or hypoxic conditions. To reach this general objective, this thesis has been structured in two main blocks: ⁻ Methodological aims: (i) We have analysed mass spectral databases for LC/MS-based untargeted metabolomics. (ii) Finally, we have generated and improved the characterization of LC/MS metabolomics data focusing on MS1 and MS2 annotation. ⁻ Biological aims: (iii) We have proposed a novel method that detects and analyses changes in protein-protein interaction (PPI) networks by hyperglycemic and/or hypoxic conditions. (iv) We have presented a novel workflow which is able to predict and validate metabolite alterations due to hyperglycemic and/or hypoxic conditions integrating protein expression data in metabolic networks.
Abdullah, Sewa Faraj. "Proteomics approaches to polyketide synthases interfaces by mass spectrometry and NMR spectroscopy and the application of chemometrics to metabolomics." Thesis, University of Bristol, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.682232.
Повний текст джерелаHamzeiy, Hamid [Verfasser], and Christoph [Akademischer Betreuer] Turck. "Advancing computational methods for mass spectrometry-based proteomics, metabolomics, and analysis of multi-omics datasets / Hamid Hamzeiy ; Betreuer: Christoph Turck." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2021. http://d-nb.info/1229835806/34.
Повний текст джерелаPoljak, Anne Medical Sciences Faculty of Medicine UNSW. "Oxidative, inflammatory and vascular factors in Alzheimer's disease." Publisher:University of New South Wales. Medical Sciences, 2008. http://handle.unsw.edu.au/1959.4/41273.
Повний текст джерелаHe, Ruifeng, Fernanda Salvato, Jeong-Jin Park, Min-Jeong Kim, William Nelson, Tiago Balbuena, Mark Willer, et al. "A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement." BioMed Central, 2014. http://hdl.handle.net/10150/610086.
Повний текст джерелаLee, Regent. "Biomarkers of coronary atherosclerotic plaque rupture." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:7f0136bf-ad55-4dc8-bcc3-1d55cb269ef8.
Повний текст джерелаAlberice, Juliana Vieira. "Avaliação analítica de potenciais biomarcadores para câncer de bexiga em urina." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-28082014-152517/.
Повний текст джерелаNesse trabalho foram utilizadas duas abordagens \'ômicas\': proteômica e metabolômica, para a busca de biomarcadores em urina para o diagnóstico e prognóstico do câncer de bexiga, respectivamente. Com a abordagem proteômica buscou-se apenas por biomarcadores para o diagnóstico da doença e, utilizando as técnicas de eletroforese 2-DE, OFFGEL e MS, juntamente com análise estatística multivariada, foi possível identificar 32 proteínas que apresentam-se como potenciais marcadores para o câncer de bexiga. A abordagem metabolômica foi empregada para a busca de biomarcadores para reincidência e progressão da doença. As técnicas analíticas utilizadas nessa abordagem, LC-MS e CE-MS, mostraram-se complementares e, dos resultados obtidos com ambas e avaliados com análise estatística multivariada foi possível indicar 19 metabólitos para reincidência e 23 metabólitos para progressão do câncer de bexiga.
Assim, neste trabalho explorou-se como as ciências \'ômicas\', a qual abrange técnicas analíticas de high-throughput, estatística multivariada e ferramentas de bioinformática auxiliando na descoberta de potenciais biomarcadores não invasivos para o diagnóstico e prognóstico do câncer de bexiga. Portanto, o presente estudo foi de grande importância e relevância à medida que ilustrou como tais técnicas podem potencialmente auxiliar no diagnóstico e prognóstico de doenças e contribuir para tratamentos personalizados no futuro, indicando a potencialidade de estudos dessa natureza.
Bladder cancer is an urogenital cancer affecting men and women, and just in Brazil 8,600 new cases are diagnosed annually. Transurethral cystoscopy is a standard conduct in the diagnosis and monitoring of bladder cancer. However, this procedure is extremely invasive, painful, expensive and does not guarantee the best results. Thus, the searching for molecular markers may assist in the diagnosis and monitoring of bladder cancer, as well as decreasing the need for invasive tests in the monitoring of patients treatment. In this way, urine shows an important role as a source of biomarkers, mainly due to its non-invasive nature.
In this work we used two \'omics\' approaches: proteomics and metabolomics, to search for biomarkers in urine for the diagnosis and progression of bladder cancer, respectively. The proteomics approach was explored for biomarkers for diagnosing disease. Using 2-DE, OFFGEL electrophoresis, and MS techniques, as well multivariate statistical analysis, they were identified 32 proteins that may be pointed as potential markers for bladder cancer. The metabolomics approach was used to search for biomarkers for progression and recurrence of the disease. The analytical techniques used for this approach, LC-MS and CE-MS, were complementary to each other and the results evaluated with multivariate statistical analysis indicated that 19 metabolites for recurrence and 23 metabolites for progression of bladder cancer could possibly be used for validation studies.
Thus, we demonstrated how the \'omics\' sciences, which include high- throughput analytical techniques, multivariate statistical analysis, and bioinformatics tools, aid in the discovery of potential biomarkers for noninvasive diagnosis, evaluate recurrence and monitor progression of bladder cancer. Therefore, this study was of high relevance to demonstrate the potential of such techniques to contribute to studies of personalized medicine.
Wu, Jikang Dr. "Mass Spectrometry-Based Metabolomics and Protein Native Structure Characterization to Improve Intervention in Salmonellosis and Proteomics-based Biomarker Characterization in Invasive Aspergillosis." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1534775122796275.
Повний текст джерелаEmami, Khoonsari Payam. "Proteomics Studies of Subjects with Alzheimer’s Disease and Chronic Pain." Doctoral thesis, Uppsala universitet, Klinisk kemi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-331748.
Повний текст джерелаBorges, Janaina de Santana. "Análise comparativa do proteoma e metaboloma de raízes de dois clones de E. grandis x E. camaldulensis, sendo um tolerante e um susceptível a condições de estresse hídrico." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-26062013-143137/.
Повний текст джерелаThe increasing demand for wood products in the domestic and international markets requires constant wood production and the Eucalyptus genus is an alternative to meet this demand. The site selection for planting this genus requires studies related to characteristics of adaptability of the species to be used. For regions with drought stress, for example, a drought resistant clone or species must be selected. Many authors have shown the potential of Eucalyptus camaldulensis and hybrids of this species with E. grandis for production in arid regions of Brazil. The adaptation of a species to a particular environment may be related to many genetic features which have an impact, for example, on the proteome and metabolome of this species. The cells in an organism have the same genome, but exhibit the most varied functions and morphologies, which are related to differences in the expression pattern of proteins and metabolites of those cells. The areas of proteomics and metabolomics can assist in the understanding the biological processes and supply an overview about plants status at any given time and in response to certain conditions / environmental stresses. The aim of this work is to perform a comparative analysis of the roots´s proteome and metabolome of two E. grandis x E. camaldulensis clones, one tolerant and another susceptible to drought, after the individuals have been subjected to different water regimes, 100% of field capacity for the control plants and 30% for the treated ones. The results showed the presence of proteins related to drought stress and the presence of secondary metabolites differentially expressed in the control when compared to treatment plants. This research has an innovative feature to be one of the first works involving proteomics and metabolomics studies of eucalyptus roots under water stress. New researches related to this field are welcome and may help to identify genes tolerant to drought stress that may be used in the future for genetic engineering plants.
Vanee, Niti. "HIGH THROUGHPUT DATA FRAMEWORK BASED CHARACTERIZATION AND EVALUATIONS OF THERMOBIFIDA FUSCA FOR INDUSTRIAL APPLICATIONS." VCU Scholars Compass, 2013. http://scholarscompass.vcu.edu/etd/3274.
Повний текст джерелаZasada, Christin. "Experimental and mathematical analysis of the central carbon metabolism in cancer and stem cells." Doctoral thesis, Humboldt-Universität zu Berlin, 2017. http://dx.doi.org/10.18452/18309.
Повний текст джерелаMetabolic reprogramming of the central carbon metabolism (CCM) is highly debated during the last decade. It describes the rearrangement of nutrient consumption for providing energy and building blocks for cellular proliferation and maintenance. So far, only sparse data are available for an in-depth analysis of metabolic reprogramming events. The herein summarised projects address metabolic programming from different perspectives and show the implementation of cell culture experiments, cutting-edge high-throughput technologies, bioinformatics, and computational modelling into one workflow providing the determination of metabolic flux maps of mammalian cells. The combination of GC-MS and LC-MS-based methodologies enable the quantitative analysis of proteins and metabolites of the CCM. Pulsed stable isotope-resolved metabolomics (pSIRM) experiments allow monitoring the fate of nutrients within the network of the CCM. The time-dependent and position-specific incorporation of carbon-13 leads to an indirect measurement of the metabolic flux, the only one functional readout of a cell. High-throughput technologies were applied in four projects to gain insights in metabolic reprogramming in cancer cell lines, human embryonic stem cells (hESCs), induced pluripotent stem (iPS) cells and their derived fibroblasts. A global principal component analysis demonstrated the discrimination of phenotypes by different classes of quantitative data. The comparison of metabolic and protein levels confirms the presence of the Warburg effect in both cell types. Though, the executing enzymes vary regarding their isoenzyme identity and expression levels. Methodological improvements provided the implementation of GC-MS derived data for INST-MFA. The mapping of GC-MS derived fragments to the molecule structure enables an extension of the CCM network. Robustness of the input data has been improved by the development of a R-scripting based quality control tool (MTXQC).
Sahlin, Sara. "The next-generation of aquatic effect-based monitoring? : A critical review about the application,challenges and barriers with omics in field." Thesis, Linnéuniversitetet, Institutionen för biologi och miljö (BOM), 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-87517.
Повний текст джерелаFranco, Flávia de Moraes. "Caracterização do proteoma nuclear e do perfil metabólico primário de folhas da cana-de-açúcar (Saccharum spp) sob condição de déficit hídrico e recuperação." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-09082016-170155/.
Повний текст джерелаSugarcane is one of the main crops in tropical countries. Brazil, besides being the world\'s largest producer of this crop, is also a leader in sugar and ethanol production. Nowadays, most of the sugarcane growing in Brazil is under rain-fed conditions, as a result, the culture is subject to water deficit at certain stages. Thus, it is essential to understand the physiological and molecular plant responses to water availability. In this context, the metabolomic and proteomic analyses aims to identify different metabolic pathways and proteins related to the mechanisms of tolerance and recovery. Samples of seven month old plants of Saccharum spp were subjected to different water conditions, deficit and rehydratation, whereas control samples were kept irrigated. The identification of primary metabolite profile was performed by Gas-Chromatography combined with Mass- Spectrometry (GC-MS). To identify nuclear proteins, the complex samples were digested and then sequenced by LC-MSE. Statistical analyses among treatments PLS-DA showed significant differences in both metabolites and proteins of Saccharum spp in different conditions. A total of 86 metabolites were identified, where 8 are preferably abundant in water stress and 10 in recovery, thus, they can be used as markers. Some of these compounds are present in common pathways like biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid and biosynthesis of phenylpropanoids. Metabolites that do not participate in these pathways, but that were at least two times more abundant in treatments when compared to control, were also discussed. They were galacturonic acid-1-phosphate, pyroglutamic acid and creatinine that were related to deficit condition and methyl dihydrogen phosphate, phosphoric acid and 2-hydroxypyridine to recovery. A total of 761 proteins were identified, of which 21 were nuclear and drought responsive and 32 were nuclear and recovery responsive. The functional classes of water stress proteins are translation and oxidation-reduction process and of recovery proteins are translation and proteolysis involved in cellular protein catabolic process. The combination of different techniques in this study revealed a complex regulatory dynamics in the mechanism of sugarcane water stress tolerance that have not been discussed in the literature.
Swensen, Adam Clayton. "Investigation of Dynamic Biological Systems Using Direct Injection and Liquid Chromatography Mass Spectrometry." BYU ScholarsArchive, 2016. https://scholarsarchive.byu.edu/etd/6574.
Повний текст джерелаJones, Christina Michele. "Applications and challenges in mass spectrometry-based untargeted metabolomics." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54830.
Повний текст джерелаMounayar, Rana. "Oral fat sensitivity in humans : links with salivary composition." Thesis, Dijon, 2013. http://www.theses.fr/2013DIJOS074.
Повний текст джерелаHuman fat perception has recently triggered particular interest as it was shown that it does not only involve aroma and texture perception but also taste perception. The latter was supported by the presence of free fatty acids (FFA) taste receptors on the tongue. Recent studies have shown that fat taste sensitivity is variable among individuals. This inter-individual variation could be linked to genetic or environmental factors. However, saliva could also play a role in this perception. The role of saliva in taste perception is increasingly recognized. Saliva contains molecules able of interacting with fat such as lipase and lipocalin. It is also a complex fluid which contains a large diversity of proteins and metabolites. Its regulation is also complex and its composition may vary after a sensory stimulation. Indeed, studies have shown that when giving primary taste stimulations, the whole salivary proteome is modified. Thus, the first aim of the present work was to use both targeted (enzymatic activity, antioxidant capacity etc) and untargeted approaches (proteomics and metabolomics) to identify links between taste sensitivity to a fatty acid, oleic acid, and the salivary composition. The second aim was to investigate whether the salivary composition is modified after an oral stimulation by oleic acid.Two groups of thirteen male subjects (highly and weakly sensitive to the taste of oleic acid) were selected from an initial panel of 73 healthy participants. Their whole saliva was collected in two ways; the first without stimulation in order to study the links between oral sensitivity to oleic acid and saliva composition and the second using a stimulation by the same fatty acid in order to study potential modifications of saliva composition depending on sensitivity. Results show that salivary composition is linked to oral fatty acid perception. Markers previously reported as associated to taste perception were determined in the highly sensitive group (carbonic anhydrase, Zinc Alpha 2 glycoprotein and cystatins) while markers (organic acids) indicating a higher bacterial load were identified in weakly sensitive group. Furthermore, results obtained after stimulation by oleic acid suggest that saliva composition is modified, which confirms its dynamic nature. As different modifications were observed for the highly and weakly sensitive group, our results suggest that saliva is not only modified after a stimulation but also depending on the sensitivity to that particular stimulation
Whitehill, Justin G. A. "INVESTIGATIONS INTO MECHANISMS OF ASH RESISTANCE TO THE EMERALD ASH BORER." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306863052.
Повний текст джерелаVvedenskaya, Olga. "An in vivo study into the metabolic reprogramming of hepatocellular carcinoma." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19268.
Повний текст джерелаThe present work evaluates the role of metabolism in development and progression of hepatocellular carcinoma (HCC). This study focuses on changes of central metabolic pathways, including glycolysis, gluconeogenesis, tricarboxylic acid (TCA) cycle and other processes involved in cellular metabolism and known to be dysregulated during cancer formation. Comprehensive multiomics analyses, such as proteomics, metabolomics and targeted genome sequencing, were applied in order to better understand HCC developmental mechanisms in vivo. Two main systems were studied: the ASV-B mouse model and clinical samples from human patients. The human cohort was composed of biopsy and surgery material from 95 patients: 47 HCC and 48 non-HCC. Proteomic data from both mice and humans show a clear downregulation of the main energy-producing pathways in HCC. Glycogen metabolism, de novo glucose synthesis, glutamine uptake to the TCA cycle, approximately 60% of enzymes of TCA cycle, and transport of pyruvate to mitochondria are downregulated in HCC. An isoform switch at various levels of central carbon metabolism was demonstrated in this work. Both mice and humans with HCC reveal isoform switches at the level of phosphoglycerate mutases and pyruvate kinases. The key features are found in both mouse and human, showing a universal metabolic HCC fingerprint. This study also demonstrates that proteomic analysis of the bioptate material is a strong and sufficient molecular diagnostic tool for research in cancer: the proteomic analysis of liver material allows the distinction of tumor samples from non-tumor samples and also to track the level of disease progression. Targeted genome sequencing revealed that no clear distinction between cancer and precancerous conditions could be made exclusively from the mutation analysis. Human metabolomic data remains inconclusive, possibly due to the different sources of tissue samples.
Harmel, Robert Klaus. "New Analytical Tools to Interrogate Inositol Pyrophosphate Signaling." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21392.
Повний текст джерелаInositol pyrophosphates (PP-InsPs) are an important group of second messengers that intersect with a wide range of processes in eukaryotic cells including phosphate homeostasis, insulin signaling and apoptosis. Despite their discovery more than two decades ago, elucidating the underlying signaling mechanisms remains a significant challenge. Therefore, a new set of chemical and analytical methods was developed here to improve our understanding of these intriguing molecules on the biochemical and cellular level. To overcome the shortage of synthetic PP-InsPs, a highly efficient and scalable chemoenzymatic approach was designed and the major mammalian PP-InsPs could be obtained in hundreds of milligram quantities and in high purity. In parallel, a new analytical tool was developed to quantify levels of PP-InsPs in complex samples. Chemoenzymatic access to 13C-labeled myo-inositol and 13C-labeled PP-InsPs enabled the detection of low concentrations of PP-InsPs using nuclear magnetic resonance spectroscopy. In vitro, these compounds were of great use for the biochemical characterization of PP-InsPs kinases from mammals and plants. Endogenous pools of PP-InsPs from human cell lines were identified and quantified by metabolic labeling with 13C-labeled myo-inositol. Finally, a new proteomics workflow towards the detection of protein pyrophosphorylation, a posttranslational modification mediated by PP-InsPs, using mass spectrometry was optimized and endogenously modified mammalian proteins could be identified for the first time and with high confidence. Taken together, the chemical and analytical tools presented here have great potential to accelerate the understanding of PP-InsP signaling and metabolism. Access to large amounts of PP-InsPs together with a reliable quantification method and the detection of endogenous protein pyrophosphorylation sites will be essential to unravel the signaling mechanisms underlying the diverse phenotypes associated with these metabolites.
Budzinski, Ilara Gabriela Frasson. "Avaliação do metabolismo primário da região cambial e casca de Eucalyptus grandis." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-02012013-172036/.
Повний текст джерелаEucalyptus genus is the most widely planted hardwood crop in the world because of its superior growth, broad adaptability and multipurpose wood properties. In today\'s \"new carbon economy\", eucalypts are receiving attention as fast-growing, short-rotation, renewable biomass crop for energy production. In spite of its economical importance, little information is available about the molecular changes that occur in primary metabolism in the wood and bark forming tissues. Furthermore, there is less information about molecular changes that occur during wood and bark formation in response to seasonal variation. It\'s known that Eucalyptus cambial region presents higher metabolic activity in summer than in winter. Thus, in order to observe the dynamic changes in transcript, protein and metabolite levels, mainly related to primary metabolism, we compared cambial tissue and bark collected in two different seasons (summer high temp + high rainfall) and winter (lower temp and little rainfall). Transcript expression patterns were analyzed by Real-Time PCR, normalization chosen by NormFinder and statistical analysis carried out using REST. The protein profile was obtained by bidimensional electrophoresis (2D-PAGE) followed by liquid chromatography associated with mass spectrometry (LC-MS/MS) and analyzed by Mascot Daemon. Metabolite profile was obtained by GC-TOF/MS, peaks were analyzed in MatLab and statistical analyses were done using SIMCA and \"R\". The results obtained with transcripts indicate differential gene expression in the cambial region and bark during summer and winter. A total of 77 and 75 proteins in cambium and bark, respectively, presented statistically significant alterations and were identified and classified into functional categories. We identified many proteins from primary metabolism. Metabolites from carbohydrate metabolism were also identified.
Correia, Barbara dos Santos. "Linking omics and ecophysiology in Eucalytus: unravelling stress tolerance in a forest species." Doctoral thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/18399.
Повний текст джерелаEucalyptus plantations are among the most productive forest stands worldwide. In Portugal and Spain, they are widely used for pulp production and as an energy crop. However, the region’s Mediterranean climate, with increasingly severe summer drought, negatively affects eucalypt growth and increases mortality. The aim of this doctoral thesis was to unravel drought tolerance in Eucalyptus globulus by investigating and interconnecting information on the processes mediating water deficit and rehydration, from gene and molecular regulation to physiological responses and plant performance, using two different genotypes and different stress trials. The thesis disclosed herein is presented in a series of research papers (chapters 2, 3, 4 and 5), preceded by a general introduction (chapter 1) and closed with concluding remarks (chapter 6). Chapter 2 describes a greenhouse trial and a slowly imposed water deficit, and is divided into three subchapters. Two genotypes (AL-18 and AL-10) were submitted to a 3-week water stress period at two different intensities (18% and 25% of field capacity), followed by 1 week of rewatering. Recovery was assessed 1 day and 1 week after rehydration. Several phytohormones were monitored in leaves, xylem sap and roots, 2 h, 4 h, 24 h, and 168 h after rewatering. Water deficit reduced height, biomass, water potential, and gas exchange. Contrarily, the levels of pigments, chlorophyll fluorescence parameters and MDA increased. ABA and ABA-GE levels increased, and JA content decreased in leaves and increased in xylem sap. During recovery, most of the physiological and biochemical responses of stressed plants were reversed. Comparative proteome (using difference gel electrophoresis) and metabolome (using gas chromatography–mass spectrometry) analyses enabled the separation and isolation of 2031 peptide spots, 217 of which were identified, and the detection of 121 polar metabolites. The analysis of the resilient clone AL-18, which presented a response network very distinct from the responsive clone AL-10, reinforced the role of specific photosynthetic and defence-related proteins as key players in mediating drought tolerance and revealed new players: glutamine synthetase, malate dehydrogenase and isoflavone reductase-like protein. Chapter 3 regards a climate chamber trial and a sudden water shortage, and is divided in two subchapters. The relative expression of 12 transcripts was analysed by quantitative PCR in two clones with different degrees of tolerance (AL-18 and AL-13) 7 and 11 days after water withholding and rehydration (2 h and 3 days after rewatering). Sudden water shortage was more detrimental to the plants than when slowly imposed, with heavier outcomes in clone AL-13, including plant death. Potential molecular indicators linked to enhanced water stress tolerance in Eucalyptus globulus were identified: rubisco activase (RCA), ferredoxin-NADP(H) oxireductase (FNR), mitochondrial malate dehydrogenase (mMDH), peroxisomal catalase (CAT) and isoflavone reductase (IFR). Afterwards, several biochemical markers of oxidative stress and DNA methylation patterns were quantified in the leaves of AL-18. The alterations detected using global and specific indicators reflected the parallel induction of redox and complex DNA methylation changes occurring during stress imposition and relief. Chapter 4 reports a field trial: the previously identified set of indicators for selection of water stress tolerance was tested in field-grown AL-18 and AL-13. Some of the plants were irrigated (IR), and others were left under environmental conditions of reduced rainfall (NI) during six and a half weeks prior to rewatering. Clone AL-18 showed few fluctuations in the conditions tested, and the alterations found in clone AL-13 highlighted an induction of photosynthetic and photorespiration metabolism after artificial rehydration. The results corroborated that responses to field conditions cannot be extrapolated from a stress applied individually in the context of developing selection markers. Chapter 5 describes a climate chamber trial that tested the isolated and combined effect of drought and heat. Physiological, biochemical and metabolomic alterations were monitored in AL-18 after a 5-day of consistent drought and/or 4 h at 40ºC. Testing drought-stressed plants subject to a heat shock revealed a decrease in gas exchange, Ψpd and JA, no alterations in electrolyte leakage, MDA, starch and pigments and increased glutathione pool in relation to control. The induction of cinnamate was a novel response triggered only by the combined stress. These results highlighted that the combination of drought and heat provides significant protection from more detrimental effects of drought-stressed eucalypts, confirming that combined stresses alter plant metabolism in a novel manner that cannot be extrapolated by the sum of the different stresses applied individually. This thesis describes a number of biological responses that enable E. globulus to thrive under conditions of water deficit and provides useful information of pathways to be explored in order to find suitable markers of abiotic stress tolerance in this species. Despite that, a bigger challenge remains and consists of the need to focus our studies in more realistic, field-like experiments, at least in the context of finding suitable selection markers in the climate change era.
As plantações de eucalipto estão entre as mais produtivas do mundo inteiro. Em Portugal e Espanha, são amplamente utilizadas na produção de polpa e como fonte de energia. No entanto, o clima mediterrânico da região, com secas de verão cada vez mais severas, afeta negativamente o crescimento do eucalipto e aumenta a sua mortalidade. Esta tese de doutoramento tem como objetivo desvendar a tolerância à seca da espécie Eucalyptus globulus, investigando e interligando informação dos processos que medeiam o défice hídrico e a reidratação, desde a regulação genética e molecular até às respostas fisiológicas e desempenho da planta, utilizando dois genótipos distintos e diferentes ensaios experimentais. Esta tese está estruturada sob a forma de estudos científicos (capítulos 2, 3, 4 e 5), precedidos por uma introdução geral (capítulo 1), e termina com as notas finais (capítulo 6). O capítulo 2 descreve um ensaio de estufa e um défice hídrico imposto lentamente, e está dividido em 3 subcapítulos. Dois genótipos (AL-18 e AL-10) foram sujeitos a um período de stress hídrico de 3 semanas com duas intensidades diferentes (18% e 25% da capacidade de campo), seguido de uma semana de reidratação. A recuperação foi avaliada um dia e uma semana depois da reidratação. Várias fitohormonas foram monitorizadas nas folhas, seiva xilémica e raízes, 2 h, 4 h, 24 h e 168 h depois da reidratação. A falta de água reduziu a altura, a biomassa, o potencial hídrico e as trocas gasosas. Pelo contrário, os níveis de pigmentos, parâmetros da fluorescência da clorofila e MDA aumentaram. Os níveis de ABA e de ABA-GE aumentaram, enquanto o JA diminuiu nas folhas e aumentou na seiva xilémica. Durante a recuperação, a maioria das alterações fisiológicas e bioquímicas provocadas pelo stress reverteram. Análises comparativas do proteoma (analisado por eletroforese em gel diferencial) e do metaboloma (analisado por cromatografia gasosa com espetrometria de massa) permitiram a separação de 2031 pontos peptídicos, dos quais 217 foram identificados, e a deteção de 121 metabolitos polares. A análise do clone resiliente AL-18, que apresentou uma rede de resposta bem distinta do clone responsivo AL-10, reforçou o papel de proteínas específicas da fotossíntese e relacionadas com a defesa como intermediários chave na tolerância à seca e revelou novos intermediários: glutamina sintetase, malato desidrogenase e isoflavona redutase. O capítulo 3 diz respeito a um ensaio em câmara climática e a uma rápida escassez de água, e está dividido em 2 subcapítulos. A expressão relativa de 12 transcritos foi analisada por PCR quantitativo em dois clones com diferentes graus de tolerância (AL-18 e AL-13) depois de 7 e 11 dias sem qualquer rega e posterior reidratação. A rápida escassez de água foi mais prejudicial para as plantas do que o défice hídrico imposto lentamente, com maior visibilidade no clone AL-13 que revelou morte de algumas plantas. Indicadores moleculares potencialmente ligados a uma tolerância aumentada foram identificados: rubisco ativase (RCA), ferredoxina-NADP(H) oxidorredutase (FNR), malato desidrogenase mitocondrial (mMDH) catalase peroxissomal (CAT) e isoflavona redutase (IFR). De seguida, vários marcadores bioquímicos de stress oxidativo e padrões de metilação do DNA foram quantificados nas folhas do clone AL-18. As alterações detetadas utilizando indicadores globais e específicos refletiram a indução de complexas modificações redox e de metilação do DNA, que ocorrem paralelamente durante a imposição e interrupção do stress. O capítulo 4 reporta um ensaio de campo: o conjunto de indicadores de seleção de tolerância hídrica identificado anteriormente foi testado em AL-18 e AL-13 plantados no campo. Algumas das plantas foram regadas artificialmente (IR) e outras foram deixadas nas condições ambientais de precipitação reduzida (NI) durante seis semanas e meia antes de voltar a regar. O clone AL-18 mostrou pouca variação nas condições testadas, e as alterações encontradas no clone AL-13 realçaram a indução do metabolismo fotossintético e fotorespiratório após a reidratação artificial. Estes resultados mostraram que as respostas das plantas no campo não podem ser extrapoladas a partir do estudo de um stress aplicado individualmente, particularmente no contexto de encontrar marcadores de seleção. O capítulo 5 descreve um ensaio em câmara climática que testou o efeito isolado e combinado de seca e calor. Alterações fisiológicas, bioquímicas e metabolómicas foram monitorizadas no clone AL-18 após 5 dias de seca consistente e/ou 4 h a 40ºC. Testar plantas em stress hídrico sujeitas a um choque térmico revelou uma diminuição das trocas gasosas, do potencial hídrico e do JA, nenhum efeito a nível da perda de eletrólitos, MDA, amido e pigmentos e um aumento na glutationa, em comparação com condições controlo. O stress combinado induziu também a produção do cinamato, uma resposta nova. Estes resultados realçam que a combinação de seca e calor fornece uma proteção significante contra os efeitos mais prejudiciais da seca isolada em eucalipto, confirmando que o stress combinado altera o metabolismo das plantas de uma forma nova que não pode ser extrapolada pela soma dos diferentes stresses aplicados individualmente. Esta tese descreve um conjunto de respostas biológicas que permitem ao eucalipto manter-se em condições de défice hídrico e revela informação útil de várias vias metabólicas a serem exploradas de modo a encontrar marcadores de tolerância ao stress abiótico apropriados. Apesar disso, um desafio maior permanece. Consiste na necessidade de focarmos os nossos estudos em experiências mais realistas, que mimetizem as condições de campo, pelo menos no contexto de encontrarmos marcadores de seleção ajustados a uma era de alterações climáticas.
Nehme, Ali. "Ciblage Tissu-Spécifique des Cascades Enzymatiques de l’Angiotensinogène dans l’Athérome Humain." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10267/document.
Повний текст джерелаAtherosclerosis remains and continues to be the leading cause of death and disability in the world. The implication of Renin-angiotensin-aldosterone system (RAAS) in the development of the disease is well experimentally and clinically documented. However, due to the complexity of the system, these studies remain dispersed and give no clear global view of the association between the system and the disease. In this regard, we studied the functional organization of a set of 37 genes encoding classical and newly discovered RAAS participants, including substrate, enzymes and receptors. This set was called extended RAAS (extRAAS). Using statistical analysis of human carotid atheroma transcriptome involving gene clustering, we revealed special features of extRAAS expression associated with atheromatous remodeling. An important feature of this pattern was the coordination of 2 clusters of genes that are known to favor atheroma formation. The first cluster constitutes genes that encode for angiotensin peptidases, including ACE, CTSG, CTSD and RNPEP. Whereas the second encode for receptors (AGTR1, MR, GR and LNPEP). We hypothesized that the local pattern of extRAAS gene expression plays a key role in the development of atherosclerosis by orienting the metabolism of active peptides
Ebah, Leonard. "Extraction and analysis of interstitial fluid, and characterisation of the interstitial compartment in kidney disease." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/extraction-and-analysis-of-interstitial-fluid-and-characterisation-of-the-interstitial-compartment-in-kidney-disease(8e9ce4a2-8ec4-41ee-ac22-bc142ca9e0b0).html.
Повний текст джерелаGüell, Cargol Marc. "Big complexity in a minimal bacterium." Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7235.
Повний текст джерелаAmb només 689 gens Mycoplasma pneumoniae es troba entre els organismes més simples que es coneixen. Degut a aquesta simplicitat, mycoplasma representa un organisme atractiu per dur a terme estudis a nivell genòmic. S'espera d'aquests treballs que pretenen descriure de manera quantitativa l'organisme sencer que ajudin a entendre els principis bàsics de la vida. Per tal l'estudiar amb profunditat del transcriptoma, s'ha fet ús d'una combinació de dades de "tiling arrays" amb especificitat de cadena, ultraseqüenciació i més de 252 microarrays. Després d'analitzar els resultats s'ha detectat una alta presència de transcrits alternatius (42%) dintre operons i una alt contingut de ARN de tipus "antisense" (89). També s'ha realitzat un estudi detallat del metabolisme. S'ha revisat i completat manualment el mapa metabòlic de M. pneumoniae, fet que ha permès el disseny d'un medi mínim amb l'ús de 19 ingredients essencials. El mapa s'ha completat amb diferents mesures d'indicadors de biomassa, metabòlits i fluxos. També s'ha estudiat la regulació de metabolisme mitjançant microarrays. Per altra banda, s'han mesurat sistemàticament les interaccions proteïna-proteïna mitjançant "Tandem affinity purification-mass spectrometry (TAP-MS)". Aquest anàlisis ha detectat 178 complexes diferents, els quals han estat complementats amb models estructurals, microscòpia electrònica i tomografia electrònica. Mitjançant la integració d'aquestes col·leccions de dades, es pot mostrar que aquest petit bacteri amaga un inesperada complexitat amb característiques com la freqüència de transcrits alternatius i ARN "antisense", una xarxa metabòlica petita però fortament controlada i una alta organització del proteoma.
Lamouche, Florian. "Analyse comparative des mécanismes de différenciation des bactéroïdes au cours des symbioses Bradyrhizobium Aeschynomene." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS036/document.
Повний текст джерелаIn case of nitrogen starvation, legume plants establish a symbiotic interaction with nitrogen-fixing soil bacteria called rhizobia. This interaction takes place in nodules where the symbionts are internalized and become bacteroids. Some legume clades also impose a differentiation process onto the bacteroids which become enlarged and polyploid, leading to elongated or spherical morphotypes. During my PhD work, I have studied bacteroid differentiation of Bradyrhizobium species in association with Aeschynomene spp.. These bacteroids display distinct differentiation levels depending on the plant host, and my analyses suggest that the most differentiated ones are also the most efficient. I investigated the bacterial factors potentially involved in the adaptations to differentiation and host-specificity, and related to the higher efficiency of the most differentiated bacteroids using global-omics approaches without a priori. The analyzed conditions were bacteroids of distinct morphotypes and free-living reference cultures. Activated functions under symbiotic conditions were identified, as well as host-specific ones. Functional analyses were performed on genes of interest. However, the bacterial mutants did not display drastic symbiotic phenotypes, showing the existence of complex gene networks leading to high resilience of rhizobial genomes
Moraes, Fabricio Edgar de. "Bioinformática aplicada à biologia sistêmica para a identificação dos fatores regulatórios do acúmulo de sacarose no colmo da cana-de-açúcar." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/95/95131/tde-01112016-112458/.
Повний текст джерелаSugarcane (Saccharum spp.) is one of the most important cultivated grasses of the world and Brazil is the largest producer, it has become an important crop due to high carbon assimilation rates allowing the synthesis and accumulation of large amounts of sucrose in their internodes. Thus, it is necessary a high understanding of the molecular mechanisms involved in the regulation of sucrose accumulation in this plant. These mechanisms have been studied at various levels, such as gene identification and localization, identification of quantitative trait locus controlling, transcriptome, proteome, characterization and metabolites identification. With all these studies is evident the necessity for a holistic approach to global understanding of the plant during the sucrose accumulation. Thus, this work aims to integrate metabolomics and proteomics data from tissues of sugarcane variety SP80-3280, during plant development and sucrose accumulation, using bioinformatics to link these results by regularized canonical correlation analysis in a systems biology approach. The results indicate differences in the metabolic and protein profile of sugarcane during development and sucrose accumulation. Metabolites classes have been proposed that may be related to sugarcane sucrose accumulation as glycerolipids, glycerophospholipids, coumarins and derivatives, steroids and steroid derivatives and fatty acyl. In addition, some proteins have been proposed that may be related to sucrose accumulation, where the most highlighted were the histones. In the biological correlations networks, have been also observed correlations between possible metabolites and proteins that can be correlated with the accumulation of sucrose in sugarcane
Juárez, Ortega Paloma. "Production of recombinant Immunoglobulin A in plants for passive immunotherapy." Doctoral thesis, Universitat Politècnica de València, 2014. http://hdl.handle.net/10251/37015.
Повний текст джерелаJuárez Ortega, P. (2014). Production of recombinant Immunoglobulin A in plants for passive immunotherapy [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/37015
TESIS
Ros, i. Simó Clara 1984. "Drug consumption and stressful experiences in adolescent mice: behavioural, neorotoxic and neurochemical responses." Doctoral thesis, Universitat Pompeu Fabra, 2013. http://hdl.handle.net/10803/111169.
Повний текст джерелаL'adolescència és un període crític en el desenvolupament de l’individu en el qual el cervell va d’un estat immadur a l’edat adulta. Aquest procés va acompanyat d’una elevada capacitat cognitiva però també de freqüents alteracions de tipus emocional, com l’ansietat o els símptomes depressius, així com la cerca de sensacions de risc. Un bon desenvolupament del cervell i del comportament es pot veure negativament afectat per factors externs com són l’abús de drogues i les condicions ambientals desfavorables. Aquest projecte consisteix en primer lloc, a estudiar l'impacte de l’alcohol en excés, la 3, 4-Metilendioximetamfetamina (MDMA) i la seva combinació en ratolins adolescents. En segon lloc, estudiar les conseqüències en l’edat adulta d’experiències estressants durant l’adolescència. Els principals resultats obtinguts referents al primer objectiu són que la combinació d'alcohol en excés i MDMA provoca alteracions de tipus emocional. Aquestes alteracions poden ser previngudes pel tractament amb antidepressius. A més, la MDMA indueix un deteriorament de la memòria que pot estar associada amb el dany oxidatiu a proteïnes específiques de l'hipocamp. També s’ha observat una resposta neuroinflamatòria en el cos estriat dels ratolins després del tractament amb MDMA, però no després del tractament amb etanol en excés. Finalment, estudis de metabolòmica indiquen que el metabolisme cerebral es veu alterat després de l’alcohol en excés, la MDMA o la seva combinació. Tot i que només són resultats preliminars, aquestes alteracions poden ser conseqüència d'un desequilibri en el metabolisme del triptòfan. Referent al segon objectiu, els nostres resultats indiquen que l'aïllament social durant l’adolescència indueix una resposta alterada a situacions novelles i estressants. Aquestes respostes anormals són probablement conseqüència d’alteracions en l’activitat de l’eix HPA.
Zang, Tuo. "Quantitative characterization of paediatric burn blister fluid." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/122968/1/Tuo_Zang_Thesis.pdf.
Повний текст джерелаMorin-Sardin, Stéphanie. "Etudes physiologiques et moléculaires de l'adaptation des Mucor aux matrices fromagères." Thesis, Brest, 2016. http://www.theses.fr/2016BRES0065/document.
Повний текст джерелаIn the cheese industry context, Mucor species exhibit an ambivalent behavior, as some species are essential technological organisms contributing to the required organoleptic characteristics of some cheeses while some others can be spoiling agents. The present study aimed at better understanding this ambivalence and investigating the putative adaptation mechanisms to cheese existing in Mucor technological species. Morphology and radial growth of 7 representative Mucor species: technological, contaminant and non-cheese related (plant endophyte) species were monitored on different media (synthetic, cheese-mimicking media and cheese) in function of key parameters for cheese manufacture (temperature, aw, pH). Cardinal values were determined on synthetic medium and as a result a predictive model was proposed and validated on cheese matrices for the temperature parameter. Interestingly, cheese technological species exhibited higher optimal growth rates on cheese related matrices than on synthetic media, while the opposite was observed for non-technological species. A comparative proteomic approach allowed unraveling the main metabolic pathways playing a role in growth of 4 of the 7 studied strains on both synthetic medium and cheese-mimicking medium. This proteomic study also highlighted the occurrence of 35 proteins specifically expressed by the technological strains M. lanceolatus UBOCC-A-109153 on the cheese-mimicking medium. Putative competitive and adaptative advantages of these hypothetical adaptation markers will be tested through additional investigations
Boutant, Marc. "Etude comparative du métabolisme des lipides chez Streptomyces coelicolor en culture avec le glucose ou le glycérol comme source de carbone." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS507.
Повний текст джерелаStreptomyces are filamentous Gram positive bacteria found in the soil upper layers. The model strain S. coelicolor M145 can produce antiobiotics and accumulate low levels of triacylglycerol when cultivated with glucose or glycerol as carbon source. This strain produces actinorhodin only when glycerol is used as carbone source, but the fatty acids accumulation as esterified oleic acid occurs with both carbon sources. However, the fatty acids accumulation only last for a short period of time, and afterward, a production of secondary metabolites is observed and the previously accumulated fatty acids are consummed. Those results suggest that the carbon source act as an effector for the production of secondary metabolites and the lipids metabolism is some ways linked to the secondary metabolism. With this work, we will try to establish the links between the primary and secondary metabolism via the lipids metabolism with S. coelicolor. First, in order to obtain cultures with an exponential growth phase and a non-exponential growth phase under a nitrogen limitation, a synthetic media and fed-batch feeding strategies have been designed. The nitrogen limitation is usually used to study lipids accumulation with oleaginous microorganisms such as Yarrowia lipilytica or Rhodotorula glutinis, and also during studies of antibiotics production with Streptomyces. The metabolomics study paired with the proteomics study made possible to establish the global directions of carbon, energy and reduced power fluxes during all the obtained growth phases. This work also showed that the microbial population is heterogeneous and 2 to 3 subpopulations can coexist with both carbon sources. The proteomics temporal changes and in particular of transciptional regulators show some late reactions to the nitrogen limitation especially when glycerol is used, probably because of the intracellular accumulation of effector compounds over time, and because of growth kinetics
Pasquier, Grégory. "Etude de l’impact de l’Esca sur la qualité des raisins par une approche protéomique." Thesis, Bordeaux 2, 2012. http://www.theses.fr/2012BOR21909/document.
Повний текст джерелаThe vine is susceptible to many fungal diseases that will affect the quality of grapes and wines. For several years, Esca has become the bane of modern viticulture because it is responsible for the death of the vines when presented in the form apoplectic. Its chronic form is associated with the presence of a procession of pathogenic fungi in the trunk and arms that will most often cause leaf symptoms. The alteration of photosynthesis that this causes will affect fruit ripening. However, little research has been conducted to characterize precisely the impact of this disease on the metabolism and chemical composition of grape berries. Our study aimed to assess, using a proteomic approach the disease's impact on the quality of grapes and get specific markers. Our work allowed us to demonstrate that the expression of foliar symptoms of Esca causes a change in abundance of proteins related to defense mechanisms and oxidative stress in the grape skin. It is also observed a change in antioxidant capacity by modulating levels of hydrogen peroxide, glutathione or pyridoxine. The concentrations of molecules of oenological interest such as amino acids, biogenic amines or tannins are also modified in the presence of foliar symptoms of Esca
Mansor, Rozaihan. "Proteomic and metabolomic studies on milk during bovine mastitis." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/3207/.
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