Статті в журналах: "Protein vibration"

1

Lu, Qin-Qin, Da-Chuan Yin, Yong-Ming Liu, Xi-Kai Wang, Peng-Fei Yang, Zheng-Tang Liu, and Peng Shang. "Effect of mechanical vibration on protein crystallization." Journal of Applied Crystallography 43, no. 3 (April 15, 2010): 473–82. http://dx.doi.org/10.1107/s0021889810009313.

Повний текст джерела

Анотація:

Mechanical vibration often occurs during protein crystallization; however, it is seldom considered as one of the factors influencing the crystallization process. This paper reports an investigation of the crystallization of five proteins using various crystallization conditions in a temperature-controlled chamber on the table of a mechanical vibrator. The results show that mechanical vibration can reduce the number of crystals and improve their optical perfection. During screening of the crystallization conditions it was found that mechanical vibration could help to obtain crystals in a highly supersaturated solution in which amorphous precipitates often normally appear. It is concluded that mechanical vibration can serve as a tool for growing optically perfect crystals or for obtaining more crystallization conditions during crystallization screening.

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Fournier, Frédéric, Elizabeth M. Gardner, Darek A. Kedra, Paul M. Donaldson, Rui Guo, Sarah A. Butcher, Ian R. Gould, Keith R. Willison, and David R. Klug. "Protein identification and quantification by two-dimensional infrared spectroscopy: Implications for an all-optical proteomic platform." Proceedings of the National Academy of Sciences 105, no. 40 (October 1, 2008): 15352–57. http://dx.doi.org/10.1073/pnas.0805127105.

Повний текст джерела

Анотація:

Electron-vibration-vibration two-dimensional coherent spectroscopy, a variant of 2DIR, is shown to be a useful tool to differentiate a set of 10 proteins based on their amino acid content. Two-dimensional vibrational signatures of amino acid side chains are identified and the corresponding signal strengths used to quantify their levels by using a methyl vibrational feature as an internal reference. With the current apparatus, effective differentiation can be achieved in four to five minutes per protein, and our results suggest that this can be reduced to <1 min per protein by using the same technology. Finally, we show that absolute quantification of protein levels is relatively straightforward to achieve and discuss the potential of an all-optical high-throughput proteomic platform based on two-dimensional infrared spectroscopic measurements.

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Nakai, Kumiko, Hideki Tanaka, Kyoko Fukuzawa, Jyunya Nakajima, Manami Ozaki, Nobue Kato, and Takayuki Kawato. "Effects of Electric-Toothbrush Vibrations on the Expression of Collagen and Non-Collagen Proteins through the Focal Adhesion Kinase Signaling Pathway in Gingival Fibroblasts." Biomolecules 12, no. 6 (June 1, 2022): 771. http://dx.doi.org/10.3390/biom12060771.

Повний текст джерела

Анотація:

Electric-toothbrush vibrations, which remove plaque, are transmitted to the gingival connective tissue via epithelial cells. Physical energy affects cell function; however, the effects of electric-toothbrush vibrations on gingival extracellular matrix (ECM) protein expression remain unknown. We aimed to examine the effects of these vibrations on the expression of ECM proteins—type I collagen (col I), type III collagen (col III), elastin, and fibronectin (FN)—using human gingival fibroblasts (HGnFs). HGnFs were seeded for 5 days in a six-well plate with a hydrophilic surface, exposed to electric-toothbrush vibrations, and cultured for 7 days. Subsequently, the mRNA and protein levels of col I, col III, elastin, and FN were examined. To investigate the role of focal adhesion kinase (FAK) signaling on ECM protein expression in vibration-stimulated cells, the cells were treated with siRNA against protein tyrosine kinase (PTK). Electric-toothbrush vibrations increased col I, col III, elastin, and FN expression; promoted collagen and non-collagen protein production; and enhanced FAK phosphorylation in HGnFs. Moreover, PTK2 siRNA completely blocked the effects of these vibrations on the expression of col I, col III and elastin mRNA. The results suggest that electric-toothbrush vibrations increase collagen, elastin, and FN production through the FAK-signaling pathway in fibroblasts.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Hassanpour, Halimeh, Vahid Niknam, and Sadaf Salami. "Acceleration Breaks the Cells Defense Mechanisms against Vibration in Anthemis gilanica Calli." International Journal of Agronomy 2021 (March 20, 2021): 1–12. http://dx.doi.org/10.1155/2021/8862860.

Повний текст джерела

Анотація:

Vibration is a mechanical stress which happens in nature and affects many biological aspects of plants. In this research, the effect of acceleration and vibration was investigated on some physiological and biochemical responses of Anthemis gilanica in vitro. Calli were induced from leaf (LS) and root segments (RS) and were applied to different frequencies of vibrations (0, 50, and 100 Hz) and accelerations (1, 2, and 4 g) on the A. gilanica calli for 30 min. Results showed that vibration significantly increased relative water content (RWC), growth parameters, protein and proline contents, ascorbate peroxidase (APX), peroxidase (POX), and superoxide dismutase (SOD) activities and decreased total carbohydrate, malondialdehyde (MDA), H2O2 contents, and polyphenol oxidase (PPO) activity in both LS and RS calli. Inversely, increase of acceleration in vibrated calli decreased growth parameters, RWC, protein content, and POX activity and induced proline and carbohydrate accumulations, SOD, APX, and PPO activities as compared to vibration alone. Different responses of two callus types were observed, and the highest growth, protein content, and membrane stability were observed in LS calli as compared to RS calli. It found that high acceleration amplitude intensified the resonance effect of vibration by induction of lipid peroxidation and oxidative stress damage in A. gilanica.

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Gronenberg, Wulfila, Ajay Raikhelkar, Eric Abshire, Jennifer Stevens, Eric Epstein, Karin Loyola, Michael Rauscher, and Stephen Buchmann. "Honeybees ( Apis mellifera ) learn to discriminate the smell of organic compounds from their respective deuterated isotopomers." Proceedings of the Royal Society B: Biological Sciences 281, no. 1778 (March 7, 2014): 20133089. http://dx.doi.org/10.1098/rspb.2013.3089.

Повний текст джерела

Анотація:

The understanding of physiological and molecular processes underlying the sense of smell has made considerable progress during the past three decades, revealing the cascade of molecular steps that lead to the activation of olfactory receptor (OR) neurons. However, the mode of primary interaction of odorant molecules with the OR proteins within the sensory cells is still enigmatic. Two different concepts try to explain these interactions: the ‘odotope hypothesis’ suggests that OR proteins recognize structural aspects of the odorant molecule, whereas the ‘vibration hypothesis’ proposes that intra-molecular vibrations are the basis for the recognition of the odorant by the receptor protein. The vibration hypothesis predicts that OR proteins should be able to discriminate compounds containing deuterium from their common counterparts which contain hydrogen instead of deuterium. This study tests this prediction in honeybees ( Apis mellifera ) using the proboscis extension reflex learning in a differential conditioning paradigm. Rewarding one odour (e.g. a deuterated compound) with sucrose and not rewarding the respective analogue (e.g. hydrogen-based odorant) shows that honeybees readily learn to discriminate hydrogen-based odorants from their deuterated counterparts and supports the idea that intra-molecular vibrations may contribute to odour discrimination.

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Goldsmith, P. C., F. Abadia Molina, C. B. Bunker, G. Terenghi, T. A. Leslie, Clare J. Fowler, J. M. Polak, and Pauline M. Dowd. "Cutaneous Nerve Fibre Depletion in Vibration White Finger." Journal of the Royal Society of Medicine 87, no. 7 (July 1994): 377–81. http://dx.doi.org/10.1177/014107689408700703.

Повний текст джерела

Анотація:

Vibration white finger or hand-arm vibration syndrome is the episodic blanching of the fingers in response to cold occurring in those who work with hand held vibrating tools. Clinically the condition differs from primary Raynaud's phenomenon as persistent paraesthesiae and pain are common in the hands and arms and these occur independently from the ‘white attacks’. Symptoms can become severe enough to warrant a change of occupation. Industrial compensation may be awarded for vibration white finger but, at present, no simple or reliable objective diagnostic test is available. Calcitonin gene-related peptide (CGRP) is a neuropeptide with powerful vasodilator properties. A deficiency of immunoreactive CGRP nerve fibres has been previously demonstrated in the digital cutaneous microvasculature of patients with primary and secondary Raynaud's phenomenon with the distribution and quantity of other types of nerve fibres not being significantly altered. To determine if the innervation of the cutaneous microvasculature in vibration white finger was also abnormal skin biopsy samples from the fingers of 15 patients with vibration white finger, six healthy age matched controls who worked with vibrating machinery and 26 healthy age matched controls who were heavy manual workers without exposure to vibrating machinery were examined by immunohistochemistry. To try to correlate any histological abnormalities with clinical neurological deficit sensory nerve conduction studies have so far been performed in six patients with vibration white finger. There was a significant reduction in both the number of CGRP and protein gene product 9.5 (PGP) immunoreactive nerve fibres in the digital cutaneous biopsies from the patients with vibration white finger when compared to the biopsies from the heavy manual workers and the healthy workers exposed to vibration. The pattern of the loss of CGRP immunoreactive nerve fibres was similar to that described previously in Raynaud's phenomenon and may account for the episodic vasospasm seen in both conditions. PGP is a constitutive protein of all nerves therefore the reduced PGP immunostaining indicates generalized structural neuronal damage which could account for the persistent pain and paraesthesiae characteristic of vibration white finger. The nerve conduction studies revealed sensory nerve action potentials within the low range of normal in five patients with vibration white finger and mild median nerve compression in the remaining patient implying that the neuronal damage in the patients with vibration white finger is confined mainly to the small unmyelinated nerve fibres. The immunohistochemical findings may provide the basis for a diagnostic test for vibration white finger.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Bodienkova, G. M., and S. I. Kurchevenko. "EVALUATION OF CYTOKINES AND HEAT SHOCK PROTEIN IN VIBRATION DISEASE." Medical Immunology (Russia) 20, no. 6 (December 15, 2018): 895–98. http://dx.doi.org/10.15789/1563-0625-2018-6-895-898.

Повний текст джерела

Анотація:

The prolonged impact of industrial vibration on workers leads to the development of a vibrational disease (VB), which occupies a leading position in the structure of occupational pathology. VB from the impact of local vibration is a chronic occupational disease characterized by a predominant lesion of the nervous, vascular system and musculoskeletal system of the upper and lower extremities. One of the real ways to reduce the incidence is the early detection of the negative impact of vibration on the body of workers. In this regard, cytokines and heat shock proteins (HSP70) can be early and sensitive indicators that reflect the severity of health disorders from exposure to vibration. The aim of the study was to study changes in the content of pro- and antiinflammatory cytokines, extracellular HSP70 and their relationship in patients with VB. In the immunological study included 43 male patients with a diagnosis of VB. The criteria for inclusion in this group were: a verified diagnosis, written informed consent to participate in the study, the harmful effects of local vibration in the workplace. According to the data of hygienic control, the working conditions of workers in dangerous occupations by vibration belong to the 4 (dangerous) class due to intensive local vibration. The content of cytokines: IL-1β, IL-8, IL-10, TNFα and HSP70 in the serum of patients was determined by enzyme immunoassay. The blood sampling for the study was taken from patients only once on admission to the hospital before the treatment. Statistical processing of data was carried out with the help of packages of application programs Statistica for Windows 6.0 and Microsoft Excel. It was established that the development of VB disease is accompanied by imbalance of the cytokine profile, characterized by a decrease in the levels of IL-1β, IL-10 and an increase in IL-8. The inhibition of production of IL-1β and IL-10 is a consequence of the chronic process that has developed in the body of the patients examined. And the revealed decrease in extracellular concentration of HSP70 in comparison with practically healthy people can be caused by the accumulation of it inside the cell or on its surface. Correlation analysis revealed the relationship between a decrease in HSP70 and an increase in IL-1β, and a decrease in IL-10 levels. Synthesis of HSP is an intracellular defense mechanism that prevents cell damage by activating the synthesis and secretion of pro-inflammatory cytokines. The obtained relationships between cytokines and HSP70 testify to the involvement of HSP70 in immunoinflammatory processes in VB. The revealed changes contribute to the chronic inflammatory process and justify the progressive course of the VB.

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Niessen, Katherine A., Mengyang Xu, Yanting Deng, Edward H. Snell, and Andrea G. Markelz. "Importance of Protein Vibration Directionality on Function." Biophysical Journal 112, no. 3 (February 2017): 353a. http://dx.doi.org/10.1016/j.bpj.2016.11.1916.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Lu, Qin-Qin, Bin Zhang, Liang Tao, Lu Xu, Da Chen, Jing Zhu, and Da-Chuan Yin. "Improving Protein Crystal Quality via Mechanical Vibration." Crystal Growth & Design 16, no. 9 (July 26, 2016): 4869–76. http://dx.doi.org/10.1021/acs.cgd.6b00227.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Sun, Chao, Ruixia Zeng, Ge Cao, Zhibang Song, Yibo Zhang, and Chang Liu. "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/919401.

Повний текст джерела

Анотація:

Recently, vibration training is considered as a novel strategy of weight loss; however, its mechanisms are still unclear. In this study, normal or high-fat diet-induced rats were trained by whole body vibration for 8 weeks. We observed that the body weight and fat metabolism index, blood glucose, triglyceride, cholesterol, and free fatty acid in obesity rats decreased significantly compared with nonvibration group(n=6). Although intrascapular BAT weight did not change significantly, vibration enhanced ATP reduction and increased protein level of the key molecule of brown adipose tissue (BAT), PGC-1α, and UCP1 in BAT. Interestingly, the adipocytes in retroperitoneal white adipose tissue (WAT) became smaller due to vibration exercise and had higher protein level of the key molecule of brown adipose tissue (BAT), PGC-1α, and UCP1 and inflammatory relative proteins, IL-6 and TNFα. Simultaneously, ATP content and PPARγprotein level in WAT became less in rats compared with nonvibration group. The results indicated that vibration training changed lipid metabolism in rats and promoted brown fat-like change in white adipose tissues through triggering BAT associated gene expression, inflammatory reflect, and reducing energy reserve.

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Di Meo, Valentina, Massimo Moccia, Gennaro Sanità, Alessio Crescitelli, Annalisa Lamberti, Vincenzo Galdi, Ivo Rendina, and Emanuela Esposito. "Probing Denaturation of Protein A via Surface-Enhanced Infrared Absorption Spectroscopy." Biosensors 12, no. 7 (July 15, 2022): 530. http://dx.doi.org/10.3390/bios12070530.

Повний текст джерела

Анотація:

We apply surface-enhanced infrared absorption (SEIRA) spectroscopy to monitor the denaturation process of a surface-bound protein A monolayer. Our proposed platform relies on a plasmonic metasurface comprising different spatial subregions (“pixels”) that are engineered to exhibit different resonances covering the infrared region of the electromagnetic spectrum that is matched to the vibrational modes of the Amide groups. Specifically, we are able to determine changes in the Amide I and Amide II vibration coupled modes, by comparing the SEIRA reflectance spectra pertaining to the native state and a denatured state induced by a pH variation. In particular, we observe some evident red-shifts in the principal Amide I mode and the Amide II vibration coupled modes (attributable to the breaking of hydrogen bonds), which result in insurmountable barriers for refolding. Thanks to the strong field localization, and consequent enhancement of the light-matter interactions, our proposed sensing platform can operate with extremely small amounts of an analyte, with an estimated detection limit of about 3 femtomoles of molecules.

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Bartel, Lee, and Abdullah Mosabbir. "Possible Mechanisms for the Effects of Sound Vibration on Human Health." Healthcare 9, no. 5 (May 18, 2021): 597. http://dx.doi.org/10.3390/healthcare9050597.

Повний текст джерела

Анотація:

This paper presents a narrative review of research literature to “map the landscape” of the mechanisms of the effect of sound vibration on humans including the physiological, neurological, and biochemical. It begins by narrowing music to sound and sound to vibration. The focus is on low frequency sound (up to 250 Hz) including infrasound (1–16 Hz). Types of application are described and include whole body vibration, vibroacoustics, and focal applications of vibration. Literature on mechanisms of response to vibration is categorized into hemodynamic, neurological, and musculoskeletal. Basic mechanisms of hemodynamic effects including stimulation of endothelial cells and vibropercussion; of neurological effects including protein kinases activation, nerve stimulation with a specific look at vibratory analgesia, and oscillatory coherence; of musculoskeletal effects including muscle stretch reflex, bone cell progenitor fate, vibration effects on bone ossification and resorption, and anabolic effects on spine and intervertebral discs. In every category research on clinical applications are described. The conclusion points to the complexity of the field of vibrational medicine and calls for specific comparative research on type of vibration delivery, amount of body or surface being stimulated, effect of specific frequencies and intensities to specific mechanisms, and to greater interdisciplinary cooperation and focus.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Barseghyan, Ernestina Kh, Hasmik M. Karapetyan, and Armen H. Trchounian. "EFFECT OF VIBRATION STRESS BOTH ON THE SHIFTS OF SOLUBLE PROTEIN FRACTIONS FROM RAT LIVER AND ON THE ARGINASE I ACTIVITY." Proceedings of the YSU B: Chemical and Biological Sciences 55, no. 1 (254) (April 28, 2021): 16–24. http://dx.doi.org/10.46991/pysu:b/2021.55.1.016.

Повний текст джерела

Анотація:

Vibration pathology is in the second place among diseases connected to the profession. The vibration effect both on the protein fraction shifts and on the activities of I type arginase (Arg) IA and IB from white rat liver has been studied in the conditions of 5-, 10- and 15-day vibration state. In the initial state of vibration some decrease of Arg IA and Arg IB activities was revealed as compared to the control without general protein spectrum change of liver extract. Along with vibration effect, the expression level of Arg IA was increased with duration enhancement, which possibly is due to hyperammonemia, in the result of which ureagenesis is stimulated. Stimulation of easy available adaptation reserves were described due to protein catabolism. Analysis of shifts of general and enzyme protein fractions in the conditions of vibration effect allows to reveal their qualitative and quantitative changes and to make important the organism adaptation reactions that result from anabolic and catabolic process relations. It is possible that the revealed changes in the activity of arginase IA and IB are a biologically expedient mechanism for regulating the activity of hepatic arginase during vibration stress.

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Barth, Andreas, and Christian Zscherp. "What vibrations tell about proteins." Quarterly Reviews of Biophysics 35, no. 4 (November 2002): 369–430. http://dx.doi.org/10.1017/s0033583502003815.

Повний текст джерела

Анотація:

1. Introduction 3702. Infrared (IR) spectroscopy – general principles 3722.1 Vibrations 3722.2 Information that can be derived from the vibrational spectrum 3722.3 Absorption of IR light 3753. Protein IR absorption 3763.1 Amino-acid side-chain absorption 3763.2 Normal modes of the amide group 3814. Interactions that shape the amide I band 3824.1 Overview 3824.2 Through-bond coupling 3834.3 Hydrogen bonding 3834.4 Transition dipole coupling (TDC) 3835. The polarization and IR activity of amide I modes 3875.1 The coupled oscillator system 3875.2 Optically allowed transitions 3885.3 The infinite parallel β-sheet 3885.4 The infinite antiparallel β-sheet 3895.5 The infinite α-helix 3906. Calculation of the amide I band 3916.1 Overview 3916.2 Perturbation treatment by Miyazawa 3936.3 The parallel β-sheet 3946.4 The antiparallel β-sheet 3956.5 The α-helix 3966.6 Other secondary structures 3987. Experimental analysis of protein secondary structure 3987.1 Band fitting 3987.2 Methods using calibration sets 4017.3 Prediction quality 4038. Protein stability 4048.1 Thermal stability 4048.2 1H/2H exchange 4069. Molecular reaction mechanisms of proteins 4089.1 Reaction-induced IR difference spectroscopy 4089.2 The origin of difference bands 4099.3 The difference spectrum seen as a fingerprint of conformational change 4109.4 Molecular interpretation: strategies of band assignment 41610. Outlook 41911. Acknowledgements 42012. References 420This review deals with current concepts of vibrational spectroscopy for the investigation of protein structure and function. While the focus is on infrared (IR) spectroscopy, some of the general aspects also apply to Raman spectroscopy. Special emphasis is on the amide I vibration of the polypeptide backbone that is used for secondary-structure analysis. Theoretical as well as experimental aspects are covered including transition dipole coupling. Further topics are discussed, namely the absorption of amino-acid side-chains, 1H/2H exchange to study the conformational flexibility and reaction-induced difference spectroscopy for the investigation of reaction mechanisms with a focus on interpretation tools.

Стилі APA, Harvard, Vancouver, ISO та ін.

15

HAMADA, Shohei, and Shin MORISHITA. "Natural vibration analysis of membrane protein as mechanosensor." Proceedings of Conference of Kanto Branch 2017.23 (2017): 1507. http://dx.doi.org/10.1299/jsmekanto.2017.23.1507.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Li, Meng C. M., Simon K.-H. Chow, Ronald M. Y. Wong, Bailing Chen, Jack C. Y. Cheng, Ling Qin, and Wing-Hoi Cheung. "Osteocyte-specific dentin matrix protein 1." Bone & Joint Research 11, no. 7 (July 1, 2022): 465–76. http://dx.doi.org/10.1302/2046-3758.117.bjr-2021-0476.r2.

Повний текст джерела

Анотація:

Aims There is an increasing concern of osteoporotic fractures in the ageing population. Low-magnitude high-frequency vibration (LMHFV) was shown to significantly enhance osteoporotic fracture healing through alteration of osteocyte lacuno-canalicular network (LCN). Dentin matrix protein 1 (DMP1) in osteocytes is known to be responsible for maintaining the LCN and mineralization. This study aimed to investigate the role of osteocyte-specific DMP1 during osteoporotic fracture healing augmented by LMHFV. Methods A metaphyseal fracture was created in the distal femur of ovariectomy-induced osteoporotic Sprague Dawley rats. Rats were randomized to five different groups: 1) DMP1 knockdown (KD), 2) DMP1 KD + vibration (VT), 3) Scramble + VT, 4) VT, and 5) control (CT), where KD was performed by injection of short hairpin RNA (shRNA) into marrow cavity; vibration treatment was conducted at 35 Hz, 0.3 g; 20 minutes/day, five days/week). Assessments included radiography, micro-CT, dynamic histomorphometry and immunohistochemistry on DMP1, sclerostin, E11, and fibroblast growth factor 23 (FGF23). In vitro, murine long bone osteocyte-Y4 (MLO-Y4) osteocyte-like cells were randomized as in vivo groupings. DMP1 KD was performed by transfecting cells with shRNA plasmid. Assessments included immunocytochemistry on osteocyte-specific markers as above, and mineralized nodule staining. Results Healing capacities in DMP1 KD groups were impaired. Results showed that DMP1 KD significantly abolished vibration-enhanced fracture healing at week 6. DMP1 KD significantly altered the expression of osteocyte-specific markers. The lower mineralization rate in DMP1 KD groups indicated that DMP1 knockdown was associated with poor fracture healing process. Conclusion The blockage of DMP1 would impair healing outcomes and negate LMHFV-induced enhancement on fracture healing. These findings reveal the importance of DMP1 in response to the mechanical signal during osteoporotic fracture healing. Cite this article: Bone Joint Res 2022;11(7):465–476.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Melentev, Andrey V., and Oleg A. Oshkoderov. "Assessment of the diagnostic significance of modern biomarkers and hemodynamic alterations in workers of vibration-hazardous occupations." HEALTH CARE OF THE RUSSIAN FEDERATION 65, no. 4 (September 7, 2021): 379–83. http://dx.doi.org/10.47470/0044-197x-2021-65-4-379-383.

Повний текст джерела

Анотація:

Introduction. One of the most significant tasks in modern labor medicine is to reduce the indicators of early disability in persons in contact with harmful and dangerous factors of the production environment. The issue of finding markers of early preclinical manifestations of vibrational disease and establishing comorbid conditions that are prognostically unfavorable for the course of the underlying occupational disease remains relevant. The purpose of the study is assessment of diagnostic significance of current neurospecific biomarkers and hemodynamic changes in workers of the profession related to vibration. Material and methods. The results of two studies were the basis for this work. The first, aimed at determining the concentration of neurospecific markers in the blood of miners, includes 154 working vibration hazardous professions. At the same time, groups were identified depending on the type of exposure to vibration: total (69 workers), local (24 workers) and combined general and local (61 miners) and control group of workers not in contact with vibration (49 people). The second study was performed to assess the change in hemodynamic parameters among 216 industrial workers, of which 114 people were in contact with vibration generating equipment, and 102 workers were included in the control group. Results. Data from the first study showed an increase in the titer of neuron-specific indicators, mainly protein S100B, depending on the type of exposure vibration and its seniority dose. The second study results indicated an increase in systolic blood pressure and total peripheral vascular resistance in miners under exposure to vibration factor. Conclusion. The results of both studies suggest that hemodynamic disorders and changes in the performance of neuro specific proteins may be interconnected. It seems advisable to continue the study in workers in vibrant occupations with comorbid pathology.

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Nishiyama, Katsuhiko. "Fine adjustments of thermo-vibrations between residues surrounding the active center in protein using dual artificial intelligence approaches and computer simulations." AIP Advances 12, no. 9 (September 1, 2022): 095305. http://dx.doi.org/10.1063/5.0102451.

Повний текст джерела

Анотація:

Adjustments of thermo-vibrations in proteins should be anticipated to provide novel helpful understanding of its properties. R111 and Q112 in the strand structure between the two domains of papain were repeatedly replaced to increase the thermo-vibration between residues surrounding its active center. This occurs due to collaborative activities composed of selections by deep neural network, verification by molecular dynamics simulations, and trainings of deep neural network by the verification results. The three replacements (R111Y-Q112K, R111C-Q112N, and R111W-Q112E) were found to increase the thermo-vibration and were expected to attain higher-temperature characteristics while at a normal temperature. The decision tree highlighted aromatic carbon and oxygen atoms in the 111th amino acid and carbon and nitrogen atoms in the 112th amino acid as influential factors. These atoms should be kept in mind to effectively carry out the collaborative activities.

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Chen, L. G., L. N. Sun, Y. X. Liu, and H. X. Wang. "A Novel Micro-Dissection Method Using Ultrasonic Vibration for Molecular Analysis." Key Engineering Materials 339 (May 2007): 291–96. http://dx.doi.org/10.4028/www.scientific.net/kem.339.291.

Повний текст джерела

Анотація:

Molecular techniques are transforming our understanding of cellular function and disease. However, accurate molecular analysis methods will be limited if the input DNA, RNA, or protein is not derived from pure population of cells or is contaminated by the wrong cells. A novel Ultrasonic Vibration Microdissection (UVM) method was proposed to procure pure population of targeted cells from tissue sections for subsequent analysis. The principle of the Ultrasonic Vibration Cutting is analyzed, and a novel microknife is designed. A multilayer piezoelectric actuator is used to actuate a sharp needle vibrating with high frequency and low amplitude (Approx. 16–50 kHz, and 0-3μm) to cut the tissue. Contrast experiment was done to test the feasibility of UVM method. Experimental results show that the embedded tissue can be quickly and precisely cut with the ultrasonic vibration micro-dissection method.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Suhr, Frank, Klara Brixius, Markus de Marées, Birgit Bölck, Heinz Kleinöder, Silvia Achtzehn, Wilhelm Bloch, and Joachim Mester. "Effects of short-term vibration and hypoxia during high-intensity cycling exercise on circulating levels of angiogenic regulators in humans." Journal of Applied Physiology 103, no. 2 (August 2007): 474–83. http://dx.doi.org/10.1152/japplphysiol.01160.2006.

Повний текст джерела

Анотація:

This study aimed to investigate the biological response to hypoxia as a stimulus, as well as exercise- and vibration-induced shear stress, which is known to induce angiogenesis. Twelve male cyclists (27.8 ± 5.4 yr) participated in this study. Each subject completed four cycle training sessions under normal conditions (NC) without vibration, NC with vibration, normobaric hypoxic conditions (HC) without vibration, and HC with vibration. Each session lasted 90 min, and sessions were held at weekly intervals in a randomized order. Five blood samples (pretraining and 0 h post-, 0.5 h post-, 1 h post-, and 4 h posttraining) were taken from each subject at each training session. Hypoxia was induced by a normobaric hypoxic chamber with an altitude of 2,500 m. The mechanical forces (cycling with or without vibration) were induced by a cycling ergometer. The parameters VEGF, endostatin, and matrix metalloproteinases (MMPs) were analyzed using the ELISA method. VEGF showed a significant increase immediately after the exercise only with exogenously induced vibrations, as calculated with separate ANOVA analysis. Endostatin increased after training under all conditions. Western blot analysis was performed for the determination of endostatin corresponding to the 22-kDa cleavage product of collagen XVIII. This demonstrated elevated protein content for endostatin at 0 h postexercise. MMP-2 increased in three of the four training conditions. The exception was NC with vibration. MMP-9 reached its maximum level at 4 h postexercise. In conclusion, the results support the contention that mechanical stimuli differentially influence factors involved in the induction of angiogenesis. These findings may contribute to a broader understanding of angiogenesis.

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Arani, A. Ghorbanpour, E. Haghparast, and Z. Khoddami Maraghi. "Vibrational response of coupled orthotropic protein microtubules immersed in cytosol considering small-scale and surface effects." Proceedings of the Institution of Mechanical Engineers, Part N: Journal of Nanomaterials, Nanoengineering and Nanosystems 231, no. 3 (June 16, 2017): 131–39. http://dx.doi.org/10.1177/2397791417712851.

Повний текст джерела

Анотація:

In this research, orthotropic Euler–Bernoulli beam and Timoshenko beam models are developed to investigate vibrational behavior of coupled protein microtubules. Microtubules are hollow cylindrical filaments in the living cells which are surrounded by filament network, which is simulated by Winkler–Riley Model. Temperature-dependent material properties for microtubules are used to study the thermal effect on vibration frequency. To apply the size effect, nonlocal theory is utilized, and the motion equations are derived based on Hamilton’s principle. In order to examine reliability of presented study, effects of various parameters such as environmental conditions, temperature change, boundary conditions and small-scale parameters on vibration characteristics of isotropic and orthotropic microtubules for both Euler–Bernoulli beam and Timoshenko beam models are discussed in detail. Results revealed that dynamic behavior of coupled microtubules is strongly dependent on the surface elasticity modulus of cytosol, so that, increasing surface elasticity modulus leads to increase in frequency of coupled microtubules. Results of this investigation can be provided as a useful reference in bio-medical clinical application.

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Haris, Parvez I. "Can infrared spectroscopy provide information on protein–protein interactions?" Biochemical Society Transactions 38, no. 4 (July 26, 2010): 940–46. http://dx.doi.org/10.1042/bst0380940.

Повний текст джерела

Анотація:

For most biophysical techniques, characterization of protein–protein interactions is challenging; this is especially true with methods that rely on a physical phenomenon that is common to both of the interacting proteins. Thus, for example, in IR spectroscopy, the carbonyl vibration (1600–1700 cm−1) associated with the amide bonds from both of the interacting proteins will overlap extensively, making the interpretation of spectral changes very complicated. Isotope-edited infrared spectroscopy, where one of the interacting proteins is uniformly labelled with 13C or 13C,15N has been introduced as a solution to this problem, enabling the study of protein–protein interactions using IR spectroscopy. The large shift of the amide I band (approx. 45 cm−1 towards lower frequency) upon 13C labelling of one of the proteins reveals the amide I band of the unlabelled protein, enabling it to be used as a probe for monitoring conformational changes. With site-specific isotopic labelling, structural resolution at the level of individual amino acid residues can be achieved. Furthermore, the ability to record IR spectra of proteins in diverse environments means that isotope-edited IR spectroscopy can be used to structurally characterize difficult systems such as protein–protein complexes bound to membranes or large insoluble peptide/protein aggregates. In the present article, examples of application of isotope-edited IR spectroscopy for studying protein–protein interactions are provided.

Стилі APA, Harvard, Vancouver, ISO та ін.

23

NAGATA, Minori, and Shin MORISHITA. "20725 Natural vibration analysis of membrane protein on cell." Proceedings of Conference of Kanto Branch 2015.21 (2015): _20725–1_—_20725–2_. http://dx.doi.org/10.1299/jsmekanto.2015.21._20725-1_.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Heireche, Houari, Abdelouahed Tounsi, Hachemi Benhassaini, Abdelnour Benzair, Malika Bendahmane, Miloud Missouri, and Sabrina Mokadem. "Nonlocal elasticity effect on vibration characteristics of protein microtubules." Physica E: Low-dimensional Systems and Nanostructures 42, no. 9 (July 2010): 2375–79. http://dx.doi.org/10.1016/j.physe.2010.05.017.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Wang, Hai-min, Xu-ping Jiang, Jian-min Ma, and Wen Zhang. "Vibration of a Single Protein Bubble in Bingham Liquid." Journal of Hydrodynamics 21, no. 5 (October 2009): 658–68. http://dx.doi.org/10.1016/s1001-6058(08)60197-3.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Baeva, Ekaterina, Roman Bleha, Markéta Sedliaková, Leonid Sushytskyi, Ivan Švec, Jana Čopíková, Ivan Jablonsky, Pavel Klouček, and Andriy Synytsya. "Evaluation of the Cultivated Mushroom Pleurotus ostreatus Basidiocarps Using Vibration Spectroscopy and Chemometrics." Applied Sciences 10, no. 22 (November 18, 2020): 8156. http://dx.doi.org/10.3390/app10228156.

Повний текст джерела

Анотація:

Fruiting bodies (basidiocarps) of the cultivated mushroom Pleurotus ostreatus (16 strains) were characterized by vibration spectroscopy and chemometrics. According to organic elemental analysis and Megazyme assay, the basidiocarps contained ~6.2–17.5% protein and ~18.8–58.2% total glucans. The neutral sugar analysis confirmed that glucose predominated in all the samples (~71.3–94.4 mol%). Fourier-transformed (FT) mid- and near-infrared (FT MIR, FT NIR) and FT Raman spectra of the basidiocarps were recorded, and the characteristic bands of proteins, glucans and chitin were assigned. The samples were discriminated based on principal component analysis (PCA) of the spectroscopic data in terms of biopolymeric composition. The partial least squares regression (PLSR) models based on first derivatives of the vibration spectra were obtained for the prediction of the macromolecular components, and the regression coefficients R2 and root mean square errors (RMSE) were calculated for the calibration (cal) of proteins (R2cal 0.981–0.994, RMSEcal ~0.3–0.5) and total glucans (R2cal 0.908–0.996, RMSEcal ~0.6–3.0). According to cross-validation (CV) diagnosis, the protein models were more precise and accurate (R2cv 0.901–0.970, RMSEcv ~0.6–1.1) than the corresponding total glucan models (R2cv 0.370–0.804, RMSEcv ~4.7–8.5) because of the wide structural diversity of these polysaccharides. Otherwise, the Raman band of phenylalanine ring breathing vibration at 1004 cm−1 was used for direct quantification of proteins in P. ostreatus basidiocarps (R ~0.953). This study showed that the combination of vibration spectroscopy with chemometrics is a powerful tool for the evaluation of culinary and medicinal mushrooms, and this approach can be proposed as an alternative to common analytical methods.

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Hortobagyi, David, Tanja Grossmann, Magdalena Tschernitz, Magdalena Grill, Andrijana Kirsch, Claus Gerstenberger, and Markus Gugatschka. "In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts." PLOS ONE 15, no. 11 (November 19, 2020): e0241901. http://dx.doi.org/10.1371/journal.pone.0241901.

Повний текст джерела

Анотація:

Introduction Voice rest following phonotrauma or phonosurgery has a considerable clinical impact, but clinical recommendations are inconsistent due to inconclusive data. As biopsies of the vocal folds (VF) for molecular biology studies in humans are unethical, we established a new in vitro model to explore the effects of vibration on human vocal fold fibroblasts (hVFF) in an inflammatory and normal state, which is based on previously published models. Methods By using a phonomimetic bioreactor we were able to apply predefined vibrational stress patterns on hVFF cultured under inflammatory or normal conditions. Inflammatory and pro-fibrotic stimuli were induced by interleukin (IL)1β and transforming growth factor (TGF)β1, respectively. Mechanical stimulation was applied four hours daily, over a period of 72 hours. Outcome measurements comprised assessment of extracellular matrix (ECM)-related components, angiogenic factors, and inflammatory and fibrogenic markers on gene expression and protein levels. Results Under inflammatory conditions, the inflammatory cytokine IL11, as well as the myofibroblast marker alpha smooth muscle actin (α-SMA) were significantly reduced when additional vibration was applied. The desirable anti-fibrotic ECM component hyaluronic acid was increased following cytokine treatment, but was not diminished following vibration. Conclusion Our experiments revealed the effect of vibrational stress on hVFF in an inflammatory state. Elevated levels of certain pro-inflammatory/pro-fibrotic factors could be mitigated by additional vibrational excitation in an in vitro setting. These findings corroborate clinical studies which recommend early voice activation following an acute event.

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Kabata-Piżuch, Anna, Agnieszka Suder, Paweł Jagielski, Katarzyna Kubasiak, Paulina Handzlik, Aneta Teległów, and Anna Marchewka. "Effect of Vibrotherapy on Body Fatness, Blood Parameters and Fibrinogen Concentration in Elderly Men." Journal of Clinical Medicine 10, no. 15 (July 23, 2021): 3259. http://dx.doi.org/10.3390/jcm10153259.

Повний текст джерела

Анотація:

Elderly people need activities that will positively contribute to a satisfactory process of getting older. Vibration training uses mechanical stimulus of a vibrational character that, similarly to other forms of physical activity, affects metabolic processes and conditions of health. The aim of this work was to assess the influence of thirty vibration treatments on body fatness, hematologic and rheologic indexes of blood, and proteinogram and fibrinogen concentration in elderly men’s blood. The study included twenty-one males, aged 60–70 years (mean age 65.3 ± 2.7), who were randomly assigned into a vibrotherapy group (VG) and took part in interventions on mattresses generating oscillatory-cycloid vibrations, and a control group (CG), without interventions. In all patients the following assessments were performed twice: an assessment of body fatness using the bioimpedance method, a complete blood count with a hematology analyzer, and erythrocyte aggregation by a laser-optical rotational cell analyzer; whereas, total plasma protein and fibrinogen values were established, respectively, by biuret and spectrophotometric methods. In order to compare the impact of vibrotherapy on changes in the analyzed variables, analysis of variance (ANOVA) or the Wilcoxon test were used. After applying thirty vibration treatments in the VG, a significant decrease in body fatness parameters was confirmed: BM (∆BM: −2.7 ± 2.0; p = 0.002), BMI (∆BMI: −0.9 ± 0.7; p = 0.002), BF (∆BF: −2.5 ± 2.5; p = 0.013), and %BF (∆%BF: −2.0 ± 2.7; p = 0.041), as well as in RBC (∆RBC: −0.1 ± 0.1; p = 0.035). However, changes in erythrocyte aggregation and proteinogram were not confirmed. It was found that after thirty treatments with VG, a significant decrease of fibrinogen level took place (∆ = −0.3 ± 0.3, p = 0.005). Application of thirty vibrotherapy treatments positively affected body fatness parameters and fibrinogen concentrations in the examined. However, further research should include a greater number of participants.

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Wang Hai-Min, Ma Jian-Min, and Zhang Wen. "Vibration of two equal-radius protein bubbles in Bingham fluid." Acta Physica Sinica 59, no. 1 (2010): 401. http://dx.doi.org/10.7498/aps.59.401.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Masnavieva, Liudmila B., I. V. Kudaeva, Y. A. Kuznetsova, and O. V. Naumova. "COMPARATIVE EVALUATION OF AUTOANTIBODIES LEVELS, REFLECTING THE CONDITION OF THE CARDIOVASCULAR SYSTEM IN PATIENTS WITH VIBRATION DISEASE DUE TO LOCAL AND COMBINED VIBRATION." Hygiene and sanitation 98, no. 10 (October 15, 2019): 1102–7. http://dx.doi.org/10.18821/0016-9900-2019-98-10-1102-1107.

Повний текст джерела

Анотація:

Introduction. Vibration disease occupies one of the leading places in the structure of occupational pathology and is caused by exposure to both local and general vibration. Since the mechanism of local and general vibration effects on the body differs, it can be assumed that their effect on the cardiovascular system will have distinctive features. The aim of the work was to study the content of specific autoantibodies, which characterize the state of the cardiovascular system, in patients with vibration disease caused by exposure to local and combined vibration. Material and methods. The relative levels of specific autoantibodies to antigens, reflecting the state of the heart and the vascular bed, were determined in the blood of persons with vibration disease caused by exposure to local and combined vibration (29 and 36 cases, respectively). Results. 18 to 26% of persons with the vibratory disease caused by the effect of combined vibration were found to have elevated levels of autoantibodies to components of the membrane and cytoplasm of myocardial cells, components of the cytoplasm of neutrophils and endothelial cells, β1-adrenoreceptors, PAPP-A, and cardiomyosin protein. In the group of individuals exposed to local vibration, the frequency of occurrence of elevated levels of antibodies by more than 20% was detected only for β1-adrenoreceptors and PAPP-A protein. Elevated levels of other indices were observed in 10-14% of individuals in this group. The levels of antibodies to components of the membrane of myocardial cells and platelets were higher in patients with the vibratory disease caused by exposure to a combined vibration than in individuals with vibratory syndrome caused by the action of local vibration. conclusion. Elevated levels of auto-AT to platelet membrane structures may be due to changes in their membranes caused by the accumulation of highly toxic lipid peroxidation metabolites.

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Lützenberg, Ronald, Kendrick Solano, Christoph Buken, Jayashree Sahana, Stefan Riwaldt, Sascha Kopp, Marcus Krüger, et al. "Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes." Cellular Physiology and Biochemistry 47, no. 4 (2018): 1729–41. http://dx.doi.org/10.1159/000491006.

Повний текст джерела

Анотація:

Background/Aims: Spaceflight negatively influences the function of cartilage tissue in vivo. In vitro human chondrocytes exhibit an altered gene expression of inflammation markers after a two-hour exposure to vibration. Little is known about the impact of long-term vibration on chondrocytes. Methods: Human cartilage cells were exposed for up to 24 h (VIB) on a specialised vibration platform (Vibraplex) simulating the vibration profile which occurs during parabolic flights and compared to static control conditions (CON). Afterwards, they were investigated by phase-contrast microscopy, rhodamine phalloidin staining, microarray analysis, qPCR and western blot analysis. Results: Morphological investigations revealed no changes between CON and VIB chondrocytes. F-Actin staining showed no alterations of the cytoskeleton in VIB compared with CON cells. DAPI and TUNEL staining did not identify apoptotic cells. ICAM-1 was elevated and vimentin, beta-tubulin and osteopontin proteins were significantly reduced in VIB compared to CON cells. qPCR of cytoskeletal genes, ITGB1, SOX3, SOX5, SOX9 did not reveal differential regulations. Microarray analysis detected 13 differentially expressed genes, mostly indicating unspecific stimulations. Pathway analyses demonstrated interactions of PSMD4 and CNOT7 with ICAM. Conclusions: Long-term vibration did not damage human chondrocytes in vitro. The reduction of osteopontin protein and the down-regulation of PSMD4 and TBX15 gene expression suggest that in vitro long-term vibration might even positively influence cultured chondrocytes.

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Wolkers, Willem F., and Harriëtte Oldenhof. "In situFTIR studies on mammalian cells." Spectroscopy 24, no. 5 (2010): 525–34. http://dx.doi.org/10.1155/2010/576151.

Повний текст джерела

Анотація:

In this work, we describe the application of FTIR spectroscopy to study lipids and proteins, in cellular systems during heating and cooling. Various spectral analysis methods are described to simultaneously study membrane phase behavior and heat-induced protein denaturation in cells. Membrane phase behavior was studied by following the temperature dependence of the CH2stretching vibration bands. Protein denaturation was studied using the amide-I and -III bands. Both amide-I and amide-III band analysis show that heat-induced denaturation of proteins within the cells is typically associated with an increase in extendedβ-sheet structures and a concomitant decrease in α-helical structures.

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Scaramozzino, Domenico, Giuseppe Lacidogna, Gianfranco Piana, and Alberto Carpinteri. "A finite-element-based coarse-grained model for global protein vibration." Meccanica 54, no. 13 (August 21, 2019): 1927–40. http://dx.doi.org/10.1007/s11012-019-01037-9.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Liu, Yan-Yan, Ying Zhang, Xin-An Zeng, H. El-Mashad, Zhong-Li Pan, and Qi-Jun Wang. "Effect of Pulsed Electric Field on Microstructure of Some Amino Acid Group of Soy Protein Isolates." International Journal of Food Engineering 10, no. 1 (February 19, 2014): 113–20. http://dx.doi.org/10.1515/ijfe-2013-0033.

Повний текст джерела

Анотація:

Abstract The effect of a pulsed electric field (PEF) on the microstructure of some amino acids was studied. Raman spectrum was used to determine the effect of PEF on tyrosine, tryptophan, proline residues, histidine, arginine, aliphatic amino acid, disulfide bond, and polypeptide backbone in soy protein isolates (SPI). Results suggested that increasing the intensity of PEF gradually to 50 kV cm−1 led to a reduction in gauche C–S conformation of CCSSCC dihedral angles. The increase of the PEF intensity caused an increase in the gauche–gauche–gauche conformation of the disulfide bond accompanying a decrease in α-helix and β-sheet and an increase in antiparallel β-sheet and disorder structure. A critical pulse intensity of 30 kV cm−1 was observed for unfolding and reassembling of SPI, which was verified in our previous study (Liu et al., Eur Food Res Technol 233:841–50). When the pulse intensity gradually increased to around 30 kV cm−1, the exposure of tyrosine and tryptophan, the vibration of CH2 wagging in proline and CH2 in the midazole ring of histidine, the vibration of C—H bending and C—N stretching inside a charged arginine, and asymmetric H—C—H bending deformation vibration in CH2 and CH3 groups in aromatic and aliphatic amino acids gradually increased, suggesting an unfolding of protein molecules. When the pulse intensity continually increased from 30 to 50 kV cm−1, the microstructure of all above amino acids decreased due to the reassembly of unfolding proteins.

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Vorobieva, Viktoriya V., Ol’ga S. Levchenkova, and Petr D. Shabanov. "Pathophysiological mechanisms of neurological disorders in experimental animals exposed to vibration." Reviews on Clinical Pharmacology and Drug Therapy 18, no. 3 (October 14, 2020): 213–24. http://dx.doi.org/10.17816/rcf183213-224.

Повний текст джерела

Анотація:

The review presents an analysis of literature on the study of nervous system changes in experimental animals exposed to vibration. The hypoxic type of cellular metabolism against the background of vibration is the result of vascular mechanoreceptors stimulation and spasm of blood vessels as well as intravascular pressure phase fluctuations and impaired blood and lymph outflow. Hemodynamic disorders and microangiopathy in the central nervous system can reach the level of cerebral vascular impairment and capillary-trophic insufficiency of the brain. Changes in calcium homeostasis at the cellular and tissue levels is a key mechanism of neuronal destruction occurring alongside with impaired blood supply to the nervous tissue in patients with vibrational disease. Long-term exposure to vibration results in reduced tissue respiration in the brain structures of experimental animals, that is most pronounced in the cortex and leads to a pathological change of spontaneous electrical activity of brain structures. Acute vibration stress stimulated the synthesis and excretion of serotonin not only in the hippocampus and hypothalamus but also in the cerebellum, which explains the disturbances of the oculomotor reactions observed in vibration exposure in the control system of the vertical vestibular ocular reflex. A decrease in the total number of neurons as well as an increase in the number of astroglia cells against the background of paravascular tissue edema were revealed against the background of changes in neurotransmitters levels. An increase in the concentration of the biomarker of structural and functional damage to brain tissue specific protein S-100B accompanies the development of professional sensorineural hearing loss and dysfunction of cerebral level vegetative regulation.

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Talaikis, Martynas, Simona Strazdaitė, Mantas Žiaunys та Gediminas Niaura. "Far-Off Resonance: Multiwavelength Raman Spectroscopy Probing Amide Bands of Amyloid-β-(37–42) Peptide". Molecules 25, № 15 (4 серпня 2020): 3556. http://dx.doi.org/10.3390/molecules25153556.

Повний текст джерела

Анотація:

Several neurodegenerative diseases, like Alzheimer’s and Parkinson’s are linked with protein aggregation into amyloid fibrils. Conformational changes of native protein into the β-sheet structure are associated with a significant change in the vibrational spectrum. This is especially true for amide bands which are inherently sensitive to the secondary structure of a protein. Raman amide bands are greatly intensified under resonance conditions, in the UV spectral range, allowing for the selective probing of the peptide backbone. In this work, we examine parallel β-sheet forming GGVVIA, the C-terminus segment of amyloid-β peptide, using UV–Vis, FTIR, and multiwavelength Raman spectroscopy. We find that amide bands are enhanced far from the expected UV range, i.e., at 442 nm. A reasonable two-fold relative intensity increase is observed for amide II mode (normalized according to the δCH2/δCH3 vibration) while comparing 442 and 633 nm excitations; an increase in relative intensity of other amide bands was also visible. The observed relative intensification of amide II, amide S, and amide III modes in the Raman spectrum recorded at 442 nm comparing with longer wavelength (633/785/830 nm) excited spectra allows unambiguous identification of amide bands in the complex Raman spectra of peptides and proteins containing the β-sheet structure.

Стилі APA, Harvard, Vancouver, ISO та ін.

37

CHITTOCK, Roger S., Simon WARD, Alan-Shaun WILKINSON, Patrick CASPERS, Barbara MENSCH, Malcolm G. P. PAGE та Christopher W. WHARTON. "Hydrogen bonding and protein perturbation in β-lactam acyl-enzymes of Streptococcus pneumoniae penicillin-binding protein PBP2x". Biochemical Journal 338, № 1 (8 лютого 1999): 153–59. http://dx.doi.org/10.1042/bj3380153.

Повний текст джерела

Анотація:

A soluble form of Streptococcus pneumoniae PBP2x, a molecular target of penicillin and cephalosporin antibiotics, has been expressed and purified. IR difference spectra of PBP2x acylated with benzylpenicillin, cloxacillin, cephalothin and ceftriaxone have been measured. The difference spectra show two main features. The ester carbonyl vibration of the acyl-enzyme is ascribed to a small band between 1710 and 1720 cm-1, whereas a much larger band at approx. 1640 cm-1 is ascribed to a perturbation in the structure of the enzyme, which occurs on acylation. The protein perturbation has been interpreted as occurring in β-sheet. The acyl-enzyme formed with benzylpenicillin shows the lowest ester carbonyl vibration frequency, which is interpreted to mean that the carbonyl oxygen is the most strongly hydrogen-bonded in the oxyanion hole of the antibiotics studied. The semi-synthetic penicillin cloxacillin is apparently less well organized in the active site and shows two partially overlapping ester carbonyl bands. The penicillin acyl-enzyme has been shown to deacylate more slowly than that formed with cloxacillin. This demonstrates that the natural benzylpenicillin forms a more optimized and better-bonded acyl-enzyme and that this in turn leads to the stabilization of the acyl-enzyme required for effective action in the inhibition of PBP2x. The energetics of hydrogen bonding in the several acyl-enzymes is discussed and comparison is made with carbonyl absorption frequencies of model ethyl esters in a range of organic solvents. A comparison of hydrolytic deacylation with hydroxaminolysis for both chymotryspin and PBP2x leads to the conclusion that deacylation is uncatalysed.

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Бородулин, Дмитрий, Dmitriy Borodulin, Татьяна Зорина, Tatyana Zorina, Виталий Иванец, Vitalij Ivanets, Екатерина Невская, et al. "Key Operation Parameters of the Vibration Mixer in the Production of Flour Baking Mixes." Food Processing: Techniques and Technology 49, no. 1 (June 26, 2019): 77–83. http://dx.doi.org/10.21603/2074-9414-2019-1-77-84.

Повний текст джерела

Анотація:

Food is one of the major health-determining factors. Thus, any product should be of high quality and contain valuable substances. Therefore, the authors selected a flour baking mix with a high content of protein to determine the key operation parameters of the vibration mixer. There have been a lot of researches that feature flour baking mixes with the help of various mixers. The present research employed an original vibration mixer of continuous action with a method of “consecutive dilution”. The experiment determined the speed of vibrotransportation of the protein mix through the operating part of the device. The power consumption of the device, as well as the speed of the vibrotransportation of the baking mix, can affect the process of mixing. Therefore, the authors studied the mutual impact of the technological parameters of the mixer and the power consumption. The analysis helped to determine the effect of the key parameters on the speed of vibrotransportation of the flour baking mix. The speed υ increased with the increase in the oscillation frequency of the operating part f and the vibration amplitude A. A high amplitude and oscillation frequency proved to increase the value of power consumption of the vibromixer. Its key operation parameters were as follows: the amplitude of vibration A = 0.0035 m, the angle of vibration β = 45°, the height of the vibroboiling layer = 0.02 m, the diameter of perforation of the operating part of the device d = 0.007 m.

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Cidem, Muharrem, Ilhan Karacan, Mehmet Cidem, Saadet Pilten Guzel, and Murat Ozkaya. "Effect of whole body vibration on the serum dentin matrix protein level." Abant Medical Journal 3, no. 2 (2014): 116–19. http://dx.doi.org/10.5505/abantmedj.2014.93064.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Fukuda, Tetsuya, Kosuke Muroda, and Hideki Kandori. "Detection of a protein-bound water vibration of halorhodopsin in aqueous solution." BIOPHYSICS 9 (2013): 167–72. http://dx.doi.org/10.2142/biophysics.9.167.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

41

NAGATA, Minori, Kazumi MATSUI, Takao SAKAI, and Shin MORISHITA. "1C21 Analysis of response of cell and membrane protein to mechanical vibration." Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2015.27 (2015): 107–8. http://dx.doi.org/10.1299/jsmebio.2015.27.107.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Moritsugu, Kei, and Jeremy C. Smith. "Temperature-Dependent Protein Dynamics: A Simulation-Based Probabilistic Diffusion-Vibration Langevin Description." Journal of Physical Chemistry B 110, no. 11 (March 2006): 5807–16. http://dx.doi.org/10.1021/jp055314t.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Pazos, Ileana M., Ayanjeet Ghosh, Matthew J. Tucker, and Feng Gai. "Ester Carbonyl Vibration as a Sensitive Probe of Protein Local Electric Field." Angewandte Chemie 126, no. 24 (April 30, 2014): 6194–98. http://dx.doi.org/10.1002/ange.201402011.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Pazos, Ileana M., Ayanjeet Ghosh, Matthew J. Tucker, and Feng Gai. "Ester Carbonyl Vibration as a Sensitive Probe of Protein Local Electric Field." Angewandte Chemie International Edition 53, no. 24 (April 30, 2014): 6080–84. http://dx.doi.org/10.1002/anie.201402011.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Machovič, V., L. Lapčák, M. Havelcová, L. Borecká, Mi Novotná, Ma Novotná, I. Javůrková, et al. "Analysis of European Honeybee (Apis Mellifera) Wings Using ATR-FTIR and Raman Spectroscopy: A Pilot Study." Scientia Agriculturae Bohemica 48, no. 1 (March 1, 2017): 22–29. http://dx.doi.org/10.1515/sab-2017-0004.

Повний текст джерела

Анотація:

Abstract The infrared (ATR-FTIR) and Raman spectroscopy was used for the structural characterization of honeybee wings. Protein, chitin, and lipid characteristic spectral features were detected using both methods. The protein secondary structure was predominantly composed of the β-sheet molecular conformation with β-turns or coil contributions. The vibration modes of the side-chain aromatic amino acid residues (tyrosine, phenylalanine, tryptophan) occurred in the wing spectra. The results of discriminant analysis showed that the infrared spectroscopy of the wing in combination with a multivariate analysis seemed promising for a resolution of the chemical structure of the wings based on lipid, proteins, and chitin content.

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Blau, Samuel M., Doran I. G. Bennett, Christoph Kreisbeck, Gregory D. Scholes, and Alán Aspuru-Guzik. "Local protein solvation drives direct down-conversion in phycobiliprotein PC645 via incoherent vibronic transport." Proceedings of the National Academy of Sciences 115, no. 15 (March 27, 2018): E3342—E3350. http://dx.doi.org/10.1073/pnas.1800370115.

Повний текст джерела

Анотація:

The mechanisms controlling excitation energy transport (EET) in light-harvesting complexes remain controversial. Following the observation of long-lived beats in 2D electronic spectroscopy of PC645, vibronic coherence, the delocalization of excited states between pigments supported by a resonant vibration, has been proposed to enable direct excitation transport from the highest-energy to the lowest-energy pigments, bypassing a collection of intermediate states. Here, we instead show that for phycobiliprotein PC645 an incoherent vibronic transport mechanism is at play. We quantify the solvation dynamics of individual pigments using ab initio quantum mechanics/molecular mechanics (QM/MM) nuclear dynamics. Our atomistic spectral densities reproduce experimental observations ranging from absorption and fluorescence spectra to the timescales and selectivity of down-conversion observed in transient absorption measurements. We construct a general model for vibronic dimers and establish the parameter regimes of coherent and incoherent vibronic transport. We demonstrate that direct down-conversion in PC645 proceeds incoherently, enhanced by large reorganization energies and a broad collection of high-frequency vibrations. We suggest that a similar incoherent mechanism is appropriate across phycobiliproteins and represents a potential design principle for nanoscale control of EET.

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Owen, Patrick J., Gabriele Armbrecht, Martin Bansmann, Jochen Zange, Regina Pohle-Fröhlich, Dieter Felsenberg, and Daniel L. Belavý. "Whey protein supplementation with vibration exercise ameliorates lumbar paraspinal muscle atrophy in prolonged bed rest." Journal of Applied Physiology 128, no. 6 (June 1, 2020): 1568–78. http://dx.doi.org/10.1152/japplphysiol.00125.2020.

Повний текст джерела

Анотація:

We examined the impact of adding protein supplementation to exercise (resistive vibration exercise) as a countermeasure against changes in the spine during spaceflight simulation. We found that adding the protein supplementation reduced spine muscle atrophy more than exercise alone. Neither countermeasure approach prevented changes in the disks in the spine or impacted back pain reports.

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Grdadolnik, Jože, and Yves Maréchal. "Hydrogen—Deuterium Exchange in Bovine Serum Albumin Protein Monitored by Fourier Transform Infrared Spectroscopy, Part I: Structural Studies." Applied Spectroscopy 59, no. 11 (November 2005): 1347–56. http://dx.doi.org/10.1366/000370205774783115.

Повний текст джерела

Анотація:

The structure of the amide I band of bovine serum albumin (BSA) was determined using an H/D exchange experiment. The difference between the dry and hydrated exchange spectrum revealed the fine structure of the amide I band. The band at 1717 ± 2 cm−1 is due to the vibration of the COOH moieties from the protein side chains. Band components at 1682 ± 2 cm−1, 1655 ± 2 cm−1, and 1637 ± 2 cm−1 are assigned to the vibrations of the backbone C=O. These three bands belong to vibrations of three different populations of amide groups differing in the number of established H-bonds. The connectivity between the frequencies of various amide vibrations was determined by two-dimensional generalized correlation spectroscopy and spectral decomposition. About 7% of the whole exchangeable hydrogen atom population (NH, NH2, and OH groups from backbone and side chains) remains unexchanged, and these hydrogen atoms belong mainly to the NH groups, which are H-bonded to specific C=O groups. Moreover, this study concerns the approximately 10% of hydrogen atoms belonging to a particular HN … OC population with a characteristic amide A frequency at 3290 cm−1 and an amide I band at 1655 ± 2 cm−1, usually attributed to the α-helical structure that remains unexchanged. At higher temperature the exchange is more efficient. Upon heating, a further 4% of these NH groups are deuterated. The comparison of the exchange spectrum at higher temperature with the structural changes of the protein at the same temperature implies that the change in overall dynamics of the protein improve the level of exchange.

Стилі APA, Harvard, Vancouver, ISO та ін.

49

Tarhan, Özgür, Enver Tarhan, and Şebnem Harsa. "Investigation of the structure of alpha-lactalbumin protein nanotubes using optical spectroscopy." Journal of Dairy Research 81, no. 1 (December 19, 2013): 98–106. http://dx.doi.org/10.1017/s0022029913000629.

Повний текст джерела

Анотація:

Alpha-lactalbumin (α-la) is one of the major proteins in whey. When partially hydrolysed with Bacillus licheniformis protease, it produces nanotubular structures in the presence of calcium ions by a self-assembly process. This study presents investigation of α-la protein structure during hydrolysis and nanotube formation using optical spectroscopy. Before spectroscopic measurements, nanotubes were examined with microscopy. The observed α-la nanotubes (α-LaNTs) were in the form of regular hollow strands with a diameter of about 20 nm and the average length of 1 μm. Amide and backbone vibration bands of the Raman spectra displayed remarkable conformational changes in α and β domains in the protein structure during nanotube growth. This was confirmed by the Fourier-transform infrared (FTIR) spectroscopy data. Also, FTIR analysis revealed certain bands at calcium (Ca++) binding sites of COO− groups in hydrolysed protein. These sites might be critical in nanotube elongation.

Стилі APA, Harvard, Vancouver, ISO та ін.

50

Shehata, Nader, Ishac Kandas, Ibrahim Hassounah, Patrik Sobolčiak, Igor Krupa, Miroslav Mrlik, Anton Popelka, Jesse Steadman, and Randolph Lewis. "Piezoresponse, Mechanical, and Electrical Characteristics of Synthetic Spider Silk Nanofibers." Nanomaterials 8, no. 8 (August 1, 2018): 585. http://dx.doi.org/10.3390/nano8080585.

Повний текст джерела

Анотація:

This work presents electrospun nanofibers from synthetic spider silk protein, and their application as both a mechanical vibration and humidity sensor. Spider silk solution was synthesized from minor ampullate silk protein (MaSp) and then electrospun into nanofibers with a mean diameter of less than 100 nm. Then, mechanical vibrations were detected through piezoelectric characteristics analysis using a piezo force microscope and a dynamic mechanical analyzer with a voltage probe. The piezoelectric coefficient (d33) was determined to be 3.62 pC/N. During humidity sensing, both mechanical and electric resistance properties of spider silk nanofibers were evaluated at varying high-level humidity, beyond a relative humidity of 70%. The mechanical characterizations of the nanofibers show promising results, with Young’s modulus and maximum strain of up to 4.32 MPa and 40.90%, respectively. One more interesting feature is the electric resistivity of the spider silk nanofibers, which were observed to be decaying with humidity over time, showing a cyclic effect in both the absence and presence of humidity due to the cyclic shrinkage/expansion of the protein chains. The synthesized nanocomposite can be useful for further biomedical applications, such as nerve cell regrowth and drug delivery.

Стилі APA, Harvard, Vancouver, ISO та ін.

Статті в журналах з теми "Protein vibration"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями