Дисертації з теми "Protein scaffolds"
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Rodriguez, Marin Silvia. "Multifunctional scaffolds for selective protein-protein inhibition." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/17299/.
Повний текст джерелаMachado, Roque Ana Isabel. "Protein scaffolds for cell culture." Thesis, University of Newcastle Upon Tyne, 2013. http://hdl.handle.net/10443/1843.
Повний текст джерелаBadger, David B. "Design and Synthesis of Protein-Protein Interaction Inhibitor Scaffolds." Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/3964.
Повний текст джерелаHaji, Ruslan Khairunnisa Nabilah. "Protein hydrogels as tissue engineering scaffolds." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/protein-hydrogels-as-tissue-engineering-scaffolds(45ff4e72-49ea-46df-9e7b-b9113576c096).html.
Повний текст джерелаWang, Hua. "Control of protein-surface, protein-protein, and cell-matrix interactions for biomaterials as tissue engineering scaffolds /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/9894.
Повний текст джерелаLu, Zhengsun. "Electrospun nanofiber scaffolds and crosslinked protein membranes as scaffold materials in tissue engineering." Thesis, Queen Mary, University of London, 2015. http://qmro.qmul.ac.uk/xmlui/handle/123456789/15023.
Повний текст джерелаLee, Maximillian. "Pyridazinediones : versatile scaffolds for site-selective protein modification." Thesis, University College London (University of London), 2018. http://discovery.ucl.ac.uk/10040797/.
Повний текст джерелаNorville, Julie Erin 1980. "Synthetic scaffolds and protein assemblies for engineering applications." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/28737.
Повний текст джерелаIncludes bibliographical references (p. 57-63).
S-layer proteins, which naturally self-assemble on the exterior of cells, provide an interesting basis for the creation of synthetic scaffolds. In this thesis, I created a plasmid which produces a recombinant form of a well characterized S layer protein, sbpA, which has a number of properties ideal for nanotechnology applications. I also explored purification of both the native and recombinant forms of sbpA. Together these preliminary studies are the first, necessary, steps towards quantitative generation of crystallization conditions and the ultimate modifications of the protein form for a wide variety of engineering applications.
by Julie Erin Norville.
S.M.
Hewitt, Sarah Helen. "Multivalent scaffolds for use as protein surface mimetics." Thesis, University of Leeds, 2017. http://etheses.whiterose.ac.uk/18027/.
Повний текст джерелаSharma, Rajan. "Protein-mediated patterning of DNA scaffolds for nanoscale electronics." Thesis, University of Leeds, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521527.
Повний текст джерелаWilson, Rowan Amelia. "Approaches to spiropiperidine scaffolds : targeting G-protein coupled receptors." Thesis, University of Southampton, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484849.
Повний текст джерелаMurphy, Natasha Susan. "Design and synthesis of novel proteomimetic scaffolds for the inhibition of protein-protein interactions." Thesis, University of Leeds, 2013. http://etheses.whiterose.ac.uk/5877/.
Повний текст джерелаKarageorgiou, Vassilis. "Bioinductive protein-based scaffolds for human mesenchymal stem cells differentiation /." Thesis, Connect to Dissertations & Theses @ Tufts University, 2004.
Знайти повний текст джерелаAdviser: David L. Kaplan. Submitted to the Dept. of Chemical and Biological Engineering. Includes bibliographical references. Access restricted to members of the Tufts University community. Also available via the World Wide Web;
Cheong, Jae Eun. "DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS." Diss., Temple University Libraries, 2016. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/406966.
Повний текст джерелаPh.D.
This research presents a new approach to creating large, complex molecules to carry out molecular recognition and catalytic functions mimicking biological proteins. Development of new therapeutics that bind protein surfaces and disrupt protein-protein interactions was first addressed targeting the envelope transmembrane protein in HIV-1, gp41. In this work, spiroligomer inhibitors of gp41 were designed and synthesized, and then the biochemical activity was tested. Rationally designed inhibitors were developed using computational modeling with the Molecular Operating Environment software (MOE). To build the desired molecular shape according to the design, C-2 alkylation of a bis-amino acid monomer was investigated to synthesize the higher degree of bis-amino acids with various reaction conditions for access to all possible diastereomers. Based on this design and synthetic methodology, a spiroligomer targeting gp41 was built by synthesizing each monomer and then linking them together by diketopiperazine (DKP). For the biological evaluation, the gp41-5 gene was transformed into E. coli and the protein was expressed, purified, and refolded for an in vitro binding test. A direct binding, fluorescence polarization assay was used to evaluate the binding affinity of the functionalized spiroligomer to the gp41-5 protein. Its antiviral activity was assessed in collaboration with the Chaiken lab at Drexel University. In addition, investigation into how the unique structures provided by the spiroligomer backbone allow for various uses, such as functionalized struts in porous organic polymers (POPs). In the large internal space of a POP, a nucleophilic, catalytic spiroligomer was installed to increase the reaction rate for the hydrolysis of methyl paraoxon (a neurotoxin G agent stimulant). Spiroligomers were designed and synthesized with backbone DMAP moieties, and the activity of these catalysts was analyzed in collaboration with the Hupp lab at Northwestern University.
Temple University--Theses
Soebbing, Samantha Lynn. "Incorporation of histidine-rich metal-binding sites onto small protein scaffolds implications for imaging, therapeutics, and catalysis /." Diss., University of Iowa, 2008. http://ir.uiowa.edu/etd/37.
Повний текст джерелаYildirim, Eda Didem Sun Wei Guceri S. I. "Plasma and protein surface functionalization for three-dimensional polycaprolactone tissue scaffolds /." Philadelphia, Pa. : Drexel University, 2010. http://hdl.handle.net/1860/3326.
Повний текст джерелаHou, Zengye. "Development of Novel Protein Kinase CK2 Inhibitors with Nitrogen Heterocyclic Scaffolds." 京都大学 (Kyoto University), 2013. http://hdl.handle.net/2433/174543.
Повний текст джерелаPettersson, Pär L. "Alpha-class glutathione transferases as steroid isomerases and scaffolds for protein redesign." Doctoral thesis, Uppsala University, Department of Biochemistry, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2034.
Повний текст джерелаThe present work focuses on the glutathione transferase (GST) Alpha-class enzymes, their characteristics as steroid isomerases and structural plasticity as malleable scaffolds for protein design. The GSTs are a family of detoxication enzymes that appears to have a wider variety of additional functions.
Kinetic steady-state parameters for human GST A1-1 with the steroid isomerase substrate Δ5-androstene-3,17-dione (AD), an intermediate in steroid hormone biosynthesis, were determined. It was established that GST A1-1 is a highly efficient steroid isomerase with a 30-fold higher catalytic efficiency, in terms of kcat/Km, than 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase, the enzyme regarded as the mammalian Δ5→4-isomerase in steroid hormone biosynthesis. Kinetic parameters were also determined for GST A2-2, GST A4-4 and the GST A1-1 mutant Y9F. From the dependency on pH of the kinetic parameters it was established that efficient catalysis requires glutathione (GSH) in its deprotonated form and it is suggested that the GSH-thiolate acts as a base in the catalysis of the Δ5→4-3-ketosteroid isomerase reaction.
GST A2-2 is a poor catalyst of the steroid isomerase reaction while GST A3-3 is highly efficient. Their catalytic efficiencies (kcat/Km) differ 5000-fold. Stepwise point mutations were performed to GST A2-2 in order to insert the amino acid residues from the active-site of GST A3-3 that distinguishes the two isoenzymes. The result was that GST A2-2 was redesigned to a highly efficient double-bond isomerase with both the catalytic constant (kcat) and catalytic efficiency (kcat/Km) in the same order as for GST A3-3. Furthermore, this was done by only exchanging amino-acid residues with first-sphere interactions, providing empirical proof-of principle for knowledge-based enzyme design.
Kinetic studies on GST A1-1 and a T68E mutant of GST A1-1 were also performed with a GSH analog lacking the g-glutamate a-carboxylate (dGSH), and using three different electrophilic substrates (AD; 1-chloro-2,4-dinitrobenzene, CDNB; 4-nitrocinnamaldehyde). Deletion of the a-carboxylate from the GSH glutamate had a severe impact on all reaction constants and it changed the rate-limiting step for the CDNB reaction as well as changed the pKa value for the enzyme-bound GSH thiol. The loss in activity caused by dGSH could in part be compensated by the T68E mutant contributing an enzyme-bound carboxylate instead.
The C-terminus of GST A1-1 is flexible and folds over the active site when the enzyme binds a substrate. Phenylalanine residues in the C-terminal end, known to interact with active-site residues tyrosine 9 and phenylalanine 10, were mutated to abolish those interactions. Studies of viscosity dependence for CDNB and AD with regard to kcat and kcat/Km showed that the dynamic C-terminal segment influence rate-determining steps for both the larger isomerase substrate, AD, as well as for the smaller conjugation substrate, CDNB.
Pettersson, Pär L. "Alpha-class glutathione transferases as steroid isomerases and scaffolds for protein redesign /." Uppsala : Acta Universitatis Upsaliensis, 2002. http://publications.uu.se/theses/91-554-5327-9/.
Повний текст джерелаGaiotto, Tiziano. "Engineering of coiled-coil protein scaffolds as innovative tools for biosensing applications." Doctoral thesis, Università degli studi di Trieste, 2009. http://hdl.handle.net/10077/3095.
Повний текст джерелаA new generation of protein scaffolds is becoming a valid alternative tool to recombinant antibodies of biotechnological, medical and pharmaceutical applications, where strong affinity and specificity are required. They share with antibodies important features (target affinity and specificity), but they have also some improvements (smaller size of molecule, tolerance to modification of the framework and the recognition site restricted to few residues), that can be exploited for biosensing application in nanotechnological platforms. Nanotechnology has been played an increasingly important role in the development of biosensors, improving the intrinsic features of biodevices. In this thesis work, we analyzed the coiled-coil domain, a widely spread dimerization domain shared by several protein scaffolds, and involved in protein-protein interaction in both eukaryotic and prokaryotic cells. The analysis of the coiled-coil structure allows a de novo design of new peptides, namely E and K, that can dimerize as a E/K coiled-coil system: the dimerization feature and the stability of the interaction makes this system an ideal platform to build up functional and customizable biosensors. A characterization of the E/K interaction was performed by using the protein complementation assay (PCA), a useful biological method to investigate the interaction between protein partners. With this in vivo method, we corroborate the interaction features determinate with circular dichroism, and we demonstrated that E and K coils effectively represent a protein scaffold, able to tolerate amino acid substitutions without altering its main structure. In addition, we create two libraries of K mutant coils, randomizing the peptide sequence, and with PCA we selected new K binders (Kran 5.17 and Krd F8) that showed a comparable interaction activity with the E-coil in preliminary in vitro tests. In the last part of this work, we generate a library of a new scaffold molecule (the single chain E-K) capable to bind small molecules as a single protein product containing both domains. Using the phage display selection system, we isolated scsE-K that can bind our analyte (the caffeine) with high specificity. This new molecules can be a powerful tool for analytical and biomedical applications.
XXI Ciclo
1980
Luisi, Immacolata. "Identification of novel protein scaffolds for small molecules binding and for catalysis." Doctoral thesis, Università degli studi di Trieste, 2012. http://hdl.handle.net/10077/7364.
Повний текст джерелаProtein scaffolds are stable structures capable to recognize and bind, in different conditions, small guest molecules. They are proteins with known conformation which can be used and modified for the construction of variants. The goal of this work is the identification of peptide-based artificial receptors or catalysts. To this purpose, we have considered two different protein motifs to generate new scaffolds: a synthetic E/K coiled-coil domain, and one of the binding sites of the natural protein Human serum albumin. In order to generate stable peptide hosts, we developed peptide libraries to be selected for both properties, adopting two different approaches: RANDOM mutagenesis and SITE-DIRECTED mutagenesis.
XXIV Ciclo
1985
Takeuchi, Tomoki. "Development of Kinesin Spindle Protein Inhibitors with Fused-indole and Diaryl Amine Scaffolds." 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/188736.
Повний текст джерелаMoaiyed, Baharlou Sogol. "ELECTRO-PROCESSED SOY PROTEIN-BASED SCAFFOLDS FOR SKIN TISSUE ENGINEERING AND WOUND HEALING." Diss., Temple University Libraries, 2017. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/480857.
Повний текст джерелаPh.D.
Wound healing is a complex, dynamic process that needs to be orchestrated in an orderly manner, involving different cell types, cytokines, and growth factors as well as extracellular matrix (ECM) interactions to avoid complications (e.g. scarring, chronic wounds, and impaired functionality). Current strategies for management of full thickness wounds are limited by material expense, limited availability of allograft tissue, autograft donor site morbidity, and even ethical problems associated with animal derived matrix components. To avoid cost, ethical, and even safety issues, there is renewed interest in using natural ingredients to construct advanced scaffolds for wound healing. At the cutting edge of the new field of regenerative medicine, demonstrated here is a scaffold based on soy protein. The second generation of soy based scaffold is constructed at the nanometer level to provide an ideal environment for cell interaction, growth, and development through a modified process termed ‘electro-processing’. The electro-processing developed here uses only water, low pressure air, and a very low current high voltage source improving not just the safety of the product, but also improving the manufacturing process. The proteins have also been indicated to release pro-healing cue to the surrounding tissue. Processing the natural soy protein to improve solubility has even enabled the new scaffolds to be generated without any harmful solvent and at rates many times faster than those previously demonstrated and at increased quality. The novel soy based electro-processed bioactive wound fabrics have been demonstrated successfully in vitro and in preliminary in vivo testing. These scaffolds have shown to be biocompatible, degradable and to improve healing quality compared to Tegaderm dressing in a rat full thickness excision model.
Temple University--Theses
Andersson, Linda K. "Exploring protein functionalisation : the site-selective modification of designed four-helix bundle scaffolds /." Göteborg : Göteborg university, 2001. http://catalogue.bnf.fr/ark:/12148/cb40110915c.
Повний текст джерелаMeinel, Lorenz. "Engineering bone and cartilage like tissue using human mesenchymal stem cells and protein scaffolds /." Aachen : Shaker, 2005. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=013174123&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Повний текст джерелаLee, Min. "Biomimetic architectural design of scaffolds and modulation of protein delivery for intestinal tissue engineering." Diss., Restricted to subscribing institutions, 2007. http://proquest.umi.com/pqdweb?did=1459913181&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.
Повний текст джерелаZeng, Like. "SELF-ASSEMBLY OF SILK-ELASTINLIKE PROTEIN POLYMERS INTO THREE-DIMENSIONAL SCAFFOLDS FOR BIOMEDICAL APPLICATIONS." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/325002.
Повний текст джерелаRashvand, Sarvenaz Nina. "SOY PROTEIN ISOLATE (SPI) “GREEN” SCAFFOLDS WITH ORIENTED MICROCHANNELS FOR APPLICATIONS IN SPINAL CORD INJURY." Master's thesis, Temple University Libraries, 2015. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/344067.
Повний текст джерелаM.S.
Every year, accidents, falls, sport injuries and other incidents cause thousands of people to suffer spinal cord injury (SCI). In the United States alone, it is estimated that the number of Americans that live with SCI is around 259,000, with 12,000 new cases that happen annually (1). These injuries lead to spinal cord damages expressed by massive nerve tract degeneration followed by neurological loss, paralysis and disabilities. Therapy of SCI patients with non-steroidal anti-inflammatory drugs (NSAIDs) help in diminishing secondary injury and lessen pain and swelling. However these drugs do not promote tissue repair. Therefore there is an unmet clinical need to develop technologies and therapeutic strategies that compensate loss of neuronal tissue, support and facilitate reestablishment of nerve tracks connectivity in the injured spinal cord. Recent progress in nerve regeneration indicates that a tissue engineering approach using soft tissue scaffolds, stem cells and neurotrophins, can lead to a partial therapy in animal models of SCI. Bioengineered scaffolds prepared by freeze casting technology provide an experimental tool for guidance of regenerating neuronal tracts and/or axons and therefore are useful for regeneration of injured spinal cord. In this engineering approach for scaffold preparation, temperature controlled directional solidification of an aqueous polymer(s) solution creates channels of different diameters that can direct axonal outgrowth of neurons populating the scaffold. In a previous study from our laboratory, such scaffolds promoted differentiation of neurons, a process facilitated by co-population of the scaffold’s channels with endothelial cells. “Green” plant proteins, such as soybean proteins, are becoming an attractive alternative source of natural polymers for a variety of biomedical applications including scaffold fabrication for neuronal tissue regeneration. In the present study, we developed a second generation of improved, microchanneled composite scaffolds from gelatin and soy protein isolate cross-linked with genipin (2 w/v %, 0.5 w/v %, 1 w/v %, respectively). The fabrication of these scaffolds by a controlled freeze drying technique, their mechanical properties (stiffness, ~3-4 kPa) as well as their uniform longitudinal channels of a diameter of ~30-55 µm is described. Preliminary biocompatibility experiments in 2D and 3D using the above mentioned scaffolds populated with either undifferentiated PC12 cells or nerve growth factor differentiated PC12 cells indicated partial biocompatibility of the scaffolds for neuronal growth. Improving the biocompatibility of these composite scaffolds is under investigation in our laboratory.
Temple University--Theses
Yan, Hongji. "New insights into principles of scaffolds design for bone application." Doctoral thesis, Uppsala universitet, Polymerkemi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-308318.
Повний текст джерелаLi, Jingxuan. "EVALUATION OF BONE MORPHOGENETIC PROTEIN-2 RELEASE FROM KERATIN SCAFFOLDS IN VITRO AND IN VIVO." Miami University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=miami1462919774.
Повний текст джерелаHulsart, Billström Gry. "Bone Regeneration with Cell-free Injectable Scaffolds." Doctoral thesis, Uppsala universitet, Ortopedi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-234846.
Повний текст джерелаHulsart, Billström Gry. "Bone Regeneration with Cell-free Injectable Scaffolds." Doctoral thesis, Uppsala universitet, Ortopedi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-310312.
Повний текст джерелаVahdati, Leïla. "Synthesis of peptidomimetics containing bifunctional diketoopiperazine scaffolds and their evaluation as modulators of amyloid-B peptide oligomerization." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA114811/document.
Повний текст джерелаThe formation of peptide and protein aggregates through the interaction of β-sheets has increasingly drawn attention since it occurs in many widespread human diseases, such as amyotrophic lateral sclerosis (ALS), Alzheimer’s disease (AD), Parkinson's disease (PD), prion diseases, and Huntington's disease (HD). Alzheimer’s disease is the most common form of dementia that causes memory loss in the elderly. In 2013, 35 million people were afflicted with AD worldwide, a number expected to double by 2050. Etiologically, the most common findings are abnormal protein deposits, including senile neuritic plaques (SNPs) and neurofibrillary tangles (NFTs). The extracellular accumulation of insoluble aggregates of β-amyloid protein (Aβ) leads to the formation of senile plaques, whereas NFTs occur intracellulary and are composed of paired helical filaments of hyperphosphorylated tau protein. Aβ peptides are produced as soluble monomers and undergo oligomerization and amyloid fibril formation via an unclear process. It is suggested that soluble A peptides play an important role in neuronal growth, survival, and synaptic modulation, while the oligomers and fibrils have toxic properties. Mimicking -strands to antagonize -sheet formation or recognition represent a new therapeutic strategy toward the prevention or treatment of diseases associated with -sheet structures such as AD. In this pathology, protein aggregation process involves a secondary structure transition from unordered/α-helix to a β-sheet rich conformation, leading to cross β-sheet structure formation. Based on the few recent published data on β-hairpin mimics, in particular on the macrocyclic structures of Nowick, as inhibitors of protein aggregation, we hypothesized that pre-structuring the peptidomimetic molecules might increase their affinity for Aβ peptides and thus increase their aggregation inhibitory activity. Our design towards a stable β-hairpin mimic (Figure 1), which could interact and eventually act as a β-sheet binder and aggregation inhibitor, involved assembling of a bifunctional diketopiperazine scaffold , a peptidomimetic strand to stabilize the formation of β-sheets and finally a suitable peptide sequence for binding to the aggregating protein. These molecules were shown to interact with the native Aβ1-42 peptide and modulate the kinetics of aggregation
Meinel, Lorenz [Verfasser]. "Engineering Bone and Cartilage-Like Tissue Using Human Mesenchymal Stem Cells and Protein Scaffolds / Lorenz Meinel." Aachen : Shaker, 2005. http://d-nb.info/1181620252/34.
Повний текст джерелаSchäfer, Gesa [Verfasser]. "Development of helix-mimetic scaffolds as potential disruptors of the interaction between protein kinase A and A kinase anchoring proteins / Gesa Schäfer." Berlin : Freie Universität Berlin, 2012. http://d-nb.info/1029937028/34.
Повний текст джерелаKutikov, Artem B. "Amphiphilic Degradable Polymer/Hydroxyapatite Composites as Smart Bone Tissue Engineering Scaffolds: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/755.
Повний текст джерелаKutikov, Artem B. "Amphiphilic Degradable Polymer/Hydroxyapatite Composites as Smart Bone Tissue Engineering Scaffolds: A Dissertation." eScholarship@UMMS, 2011. http://escholarship.umassmed.edu/gsbs_diss/755.
Повний текст джерелаDe, Raffele Daria. "Computational studies of the Retro-Aldol reaction catalyzed by different protein scaffolds. Towards the redesign of an improved enzyme." Doctoral thesis, Universitat Jaume I, 2022. http://dx.doi.org/10.6035/14122.2022.709183.
Повний текст джерелаPrograma de Doctorat en Química Teòrica i Modelització Computacional
Takahashi, Yoshitake. "MATERIAL DESIGN OF BIODEGRADABLE CELL SCAFFOLDS FOR CONTROLLED RELEASE OF BONE MORPHOGENETIC PROTEIN-2 AND THE BONE REGENERATION POTENTIAL." Doctoral thesis, 京都大学 (Kyoto University), 2007. http://hdl.handle.net/2433/44145.
Повний текст джерелаGENERAL INTRODUCTION: Bone reconstruction is a clinically important procedure to treat bone defects and has been widely tried by different methods. Basically, bone has the inherent ability to spontaneously repair itself for the bone fracture of small size. However, such a self-repairing cannot always be expected for large-size defects that are caused by trauma, tumor resection, spinal arthodesis, and congenital abnormalities. This situation often happens clinically and the therapeutic demand has been being increased recently [1]. Autograft, which is considered to be a gold standard as bone substitutes, is applied to the defect site because it provides a suitable environment for cell attachment, proliferation, and differentiation for bone regeneration [2]. However, it has several disadvantages, such as the limited donor supply, potential complications with chronic pain at the donor sites [3, 4]. On the other hand, allograft is being performed clinically [2], but the rate of graft integration into the surrounding natural bone is lower than that of autograft. In addition, it is necessary for the allograft to consider a risk of disease transmission and postoperative complications due to the tissue rejection [4, 5]. Therefore, under these circumstances, as the substitute for the bone grafts, the biomaterials of metals and ceramics have been investigated and developed. Although the above problems may be cleared, they have other disadvantages, such as the lack of biodegradability under physiological conditions and the limited processability [6]. Especially, metals show poor integration property to the bone tissue at the implantation site compared with the autograft and allograft although they provide mechanical support [7]. Different from artificial biomaterials, one of the important advantages for the bone graft is to positively accelerate osteoconduction and osteoinduction. As one trial to tackle and improve the points to be resolved, bone tissue engineering has been attracted much attention as a new therapeutic technology [8-11]. The basic idea is to provide key cells the local environment suitable to promote their proliferation and differentiation for the induction of tissue regeneration. (...) In summary, this thesis describes the feasibility of gelatin-based scaffolds in the controlled release carrier of BMP-2 and the three-dimensional matrix of MSC for osteogenic differentiation. It is concluded that this material design of scaffold is promising to effectively induce bone regeneration based on tissue engineering.
京都大学
0048
新制・論文博士
博士(工学)
乙第12103号
論工博第3968号
新制||工||1418(附属図書館)
UT51-2007-M983
(主査)教授 田畑 泰彦, 教授 岩田 博夫, 教授 木村 俊作
学位規則第4条第2項該当
Doctor of Engineering
Kyoto University
DFAM
Sheremet, Andriy. "Bioinspired polyethersulfone-based hollow fiber membranes as the scaffolds in renal assist device for protein-bound toxins removal from blood." Master's thesis, Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/13308.
Повний текст джерелаErasmus Mundus Master in Membrane Engineering
Using bioartificial kidney is the promising approach for removal of non-dializable, proteinbound uremic toxins, which are responsible for high mortality and morbidity in treating kidney failure related conditions. Additionaly, bioartificial kidney device could perform the physiological roles of the kidney such as metabolic replacement, endocrine function and immunomodulation. In the current work two commercial polyethersulfone-based membranes, Gambro HCO 1100 and Membrana MicroPES TF10 used in haemofiltration and plasma separation applications respectively were investigated. To provide adequate cytocompatibility of the membrane biomimetic, biomimetic double layer coating was developed. First, the membranes were coated with musselinspired synthetic polydopamine film, following with the coating of Collagen Type IV. Transport properties of the coated and native membranes were investigated. Increase in pure water permeability of the coated HCO 1100 membranes was observed. Membrane surface hydrophilization was assumed as the major factor responsible for the effect. Membrane permeabilities for bovine serum albumin and immunoglobulin G solutions were studied. Significant increase in protein rejection was observed for double coated HCO 1100 membranes with small or no effect of the double coated MicroPES TF10 membranes. Next, formation of confluent monolayers of the renal epithelial cells on the membrane scaffolds was studied. Cell seeding strategy was developed and two seeding conditions were tested. Specifically, the cells were allowed to adhere to the biomimetic membranes passively, and the negative pressure was applied to facilitate cell adhesion. After cultivation in semi-batch conditions the monolayer formation was examined. Confluent monolayers were observed for the conditions with passive cell adherence for the both membranes. Cell contacts formation and cell polarization were confirmed with the staining for ZO-1 protein. Applying the pressure to facilitate cell adhesion, on the contrary, resulted in the loss of cell ability to form functional monolayers.
EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universities
Caves, Jeffrey Morris. "Architecturally defined scaffolds from synthetic collagen and elastin analogues for the fabrication of bioengineered tissues." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/31731.
Повний текст джерелаCommittee Chair: Elliot L. Chaikof; Committee Member: Ajit Yoganathan; Committee Member: Larry McIntire; Committee Member: Marc Levenston; Committee Member: Mark Allen. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Ruiz-Gómez, Gloria, John C. Hawkins, Jenny Philipp, Georg Künze, Robert Wodtke, Reik Löser, Karim Fahmy, and M. Teresa Pisabarro. "Rational Structure-Based Rescaffolding Approach to De Novo Design of Interleukin 10 (IL-10) Receptor-1 Mimetics." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-215877.
Повний текст джерелаRuiz-Gómez, Gloria, John C. Hawkins, Jenny Philipp, Georg Künze, Robert Wodtke, Reik Löser, Karim Fahmy, and M. Teresa Pisabarro. "Rational Structure-Based Rescaffolding Approach to De Novo Design of Interleukin 10 (IL-10) Receptor-1 Mimetics." Public Library of Science, 2016. https://tud.qucosa.de/id/qucosa%3A30052.
Повний текст джерелаKwan, Ann H. Y. "Protein design based on a PHD scaffold." Connect to full text, 2004. http://setis.library.usyd.edu.au/adt/public_html/adt-NU/public/adt-NU20041202.102526/index.html.
Повний текст джерелаChapter headings on separately inserted unnumbered cream coloured leaves. Bibliography: leaves 122-135.
Ham, Trevor Richard. "Covalent Growth Factor Tethering to Guide Neural Stem Cell Behavior." University of Akron / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=akron1555347467862553.
Повний текст джерелаFeng, Wei. "Structural studies of supramolecular complex assembly by neuronal scaffold proteins /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BICH%202005%20FENG.
Повний текст джерелаMatskova, Liudmila V. "EBV membrane protein LMP2A interactions with ubiquitin ligases and signaling scaffold /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-068-0/.
Повний текст джерелаKnave, Axel. "Production and characterization of alternative scaffold proteins for medical applications." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278838.
Повний текст джерелаAntikroppar, som föregångare inom området biologiska läkemedel, är ursprungligen en organisms svar på invasionen av olika patogen. Idag är antikroppar en vanlig behandling för många kroniska sjukdomar, såsom de immunmedierade inflammatoriska sjukdomarna reumatoid artrit eller psoriasis. Cytokinerna interleukin 17a (IL17a) och interleukin 17c (IL17c) tros vara involverade i dessa sjukdomar och behandlas vanligtvis med antikroppar som hämmar cytokinerna. Trots att antikroppar har varit en stor framgång som biologiska läkemedel har de också nackdelar när det gäller deras produktion, storlek och stabilitet. För att hitta alternativ till antikroppar inom diagnostik och terapi har en ny klass av biologiska läkemedel utvecklats. Så kallade alternative scaffold proteins är små polypeptidkedjor som kan manipuleras för att visa affinitet gentemot olika biomarkörer. ABD-Derived Affinity ProTeins eller ADAPTs är ett exempel på dessa alternative scaffolds som kan modifieras för att binda en biomarkör som mål utan att påverka affiniteten till Humant Serum Albumin (HSA), vilket gör dem bispecifika. I detta projekt klonades, producerades, renades och karakteriserades tjugofyra tidigare utvalda ADAPT-bindarkandidater som har visat goda förutsättningar gentemot IL17a och IL17c i tidigare experiment. Proteinerna producerades i E. coli, renades genom affinitetskromatografi och karakteriserades med användning av Surface Plasmon Resonance (SPR), Circular Dichroism (CD) och Size Exclusion Chromatography (SEC). Alla kandidater klonades framgångsrikt i E. coli och av dessa kunde 10 produceras. Fem bindare visade affinitet till deras mål med SPR. Undersökning med SEC och CD visade dock att proteinvarianterna inte var strukturellt stabila och antydan till föroreningar kunde detekteras i proverna. Detta och ett lågt utbyte kunde ytterligare bekräftas via SDS-PAGE. Sammanfattningsvis kunde bindare producerades och dessa kan teoretiskt vara lovande kandidater till diagnostik eller terapi av kroniska sjukdomar där IL17a och/eller IL17c är viktiga. För att stödja dessa påståenden krävs dock ytterligare experiment och utveckling av bindarna.
Kouvatsos, Nikolaos. "Characterisation of rabbit ileal lipid binding protein and design of new β-scaffold proteins." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442293.
Повний текст джерелаKantamneni, Sriharsha. "Identification and characterisation of proteins interacting with novel GABAβ receptor interacting scaffold protein (GISP)." Thesis, University of Bristol, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.412275.
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