Дисертації з теми "Protein and mRNA levels"
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Simion, Oana-Maria. "Uncoupling proteins mRNA levels in mice lacking acylation-stimulating protein." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33018.
Повний текст джерелаIn male ASP-deficient mice mRNA levels were measured by semi-quantitative RT-PCR and the following changes were observed: UCP-1 decreased in all tested tissues, UCP-2 increased by 15% and 6 fold in muscle and white adipose tissue and UCP-3 increased 2.5 and 10 fold in muscle and epididymal adipose tissue, respectively. In female ASP-deficient mice UCP-1 decreased in all tissues, UCP-2 increased by 10% and 40% in inguinal and brown adipose tissue, respectively, and UCP-3 remained stable in all tissues. High fat diet nullified these differences, and decreased all wild-type UCP levels.
We propose that UCP-2 and 3 assume the role of UCP-1 in fuel utilization, thus helping mice face an increased energy load in the absence of ASP.
Byström, Jonas. "Eosinophil Cationic Protein : Expression Levels and Polymorphisms." Doctoral thesis, Uppsala University, Department of Medical Sciences, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2059.
Повний текст джерелаThe eosinophil cationic protein (ECP) is usually associated with the eosinophil granulocyte. In this thesis the presence and production of this protein has been studied in two other cells. The circulating monocyte was found to contain ECP mRNA and small amounts of ECP, one thousand times less than that found in the eosinophil. The production decreased by differentiation of the myelomonoblastic cell line U937 into a macrophage phenotype. Submucosal lung macrophages did not stain for ECP and alveolar macrophages did not contain ECP mRNA. The circulating neutrophil contains ECP at a level hundred fold less than the eosinophil. We found that the protein is located to the primary granules of the neutrophil but could detect no ECP mRNA in the cell. It was shown in vitro that the protein was taken up by the cell and partly transported to the primary granules. The uptake did not seem to be receptor mediated. Upon stimulation of the neutrophils, ECP previously taken up, was re-secreted.
The ECP protein is heterogeneous both to molecular characteristics and to function. To evaluate if a genetic component is involved, the ECP gene was analysed in 70 individuals. Three single nucleotide polymorphisms (SNP´s) were found, denoted 277(C>T), 434(G>C) and 562(G>C). The two first were located to the mature peptide-coding region and would change the amino acids, arg45cys and arg97thr. The prevalence of the most common SNP, 434, was evaluated in two eosinophil-related diseases, allergy/asthma and Hodgkin Lymphoma (HL). Forty-three HL patients were evaluated and it was found that the 434GG was significantly more prevalent in patients having nodular sclerosis (NS) as compared to other histologies (p=0.03). Erythrocyte sedimentation rate was also related to the 434GG genotype (p=0.009). In 209 medical students 434GG was more common (p=0.002) in those who indicated allergy. The genotype was unrelated to the production of IgE antibodies to allergens. In analysis of 76 subjects with asthma it was found that the 434GG genotype was significantly more common among allergic asthmatics (p=0.04). Asthma and HL-NS are characterized by fibrosis and eosinophils and ECP has been suggested in fibrosis development.
Kachra, Zarin. "Regulation of insulin-like growth factor-I (IGF-I) and IGF-binding protein-1 (IGFBP-1) mRNA levels in cultured rat hepatocytes." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41300.
Повний текст джерелаIGF-I mRNA levels were stimulated 2.0 to 2.5 fold by bovine growth hormone (bGH) and 1.8 to 2.0 fold by glucagon but on combining bGH and glucagon, a synergistic effect was observed and IGF-I mRNA level was augmented 10 to 12 fold. Octreotide blocked the hGH induced stimulation of IGF-I production in serum and hepatic IGF-I mRNA levels in hypophysectomized rats. This effect could have been partly due to the low levels of glucagon in serum when hypophysectomized rats were treated with hGH and octreotide. Octreotide was also found to inhibit GH stimulated IGF-I mRNA levels in rat hepatocytes.
The unique synergy observed with glucagon and bGH on IGF-I mRNA levels in hepatocytes was not reproduced by T$ sb3$, oPRL, dexamethasone, EGF or insulin when each was added in combination with bGH or glucagon. Like glucagon, the addition of IBMX or (Bu)$ sb2$cAMP stimulated IGF-I mRNA levels 1.8 to 2.0 fold, but in the presence of bGH, IGF-I mRNA levels were stimulated 10 to 12 fold. PMA stimulated IGF-I mRNA levels 1.2 to 1.4 fold but displayed no synergism when added with bGH. The stimulatory effect of bGH plus glucagon on IGF-I mRNA levels was inhibited in PKC depleted cells, in the presence of inhibitors of PKC and in the presence of cycloheximide. bGH had no posttranscriptional effect on IGF-I mRNA stability whereas glucagon or (Bu)$ sb2$cAMP stabilized IGF-I mRNA at a posttranscriptional level.
In summary, the major hormonal regulators of hepatic IGF-I mRNA levels appear to be GH and glucagon. Hepatic IGF-I mRNA levels are regulated by pathways involving protein kinase C and, protein kinase A as well as by synthesis of one or more protein(s).
Glucagon and dexamethasone each stimulated IGFBP-1 mRNA levels 3 to 4 fold whereas bGH and T$ sb3$ each inhibited IGFBP-1 mRNA levels 45 to 70%. Insulin, which inhibited IGFBP-1 mRNA levels 95%, was the most powerful inhibitor and was also found to inhibit IGFBP-1 mRNA levels in the presence of dexamethasone. IBMX and (Bu)$ sb2$cAMP stimulated IGFBP-1 mRNA levels 6 to 8 fold whereas PMA inhibited IGFBP-1 mRNA levels 40 to 50%. The inhibitory effect of bGH on IGFBP-1 mRNA levels was abolished in PKC depleted cells and also in the presence of inhibitors of PKC. In the presence of cycloheximide, IGFBP-1 mRNA was superinduced by bGH. bGH had no posttranscriptional effect on IGFBP-1 mRNA whereas glucagon and (Bu)$ sb2$cAMP stabilized IGFBP-1 mRNA at a postranscriptional level.
In summary, bGH, T$ sb3$ and insulin inhibited whereas dexamethasone and glucagon stimulated IGFBP-1 mRNA levels in hepatocytes. Effect of glucagon may be via elevation of cAMP levels, whereas the effect of bGH may be via activation of PKC levels. The inhibitory effect of bGH appears to require synthesis of one or more protein(s) besides stimulation of PKC levels.
Schwanhäußer, Björn. "Global analysis of cellular protein dynamics by pulse-labeling and quanti tati ve mass spectrometry." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16305.
Повний текст джерелаThe first part of the thesis describes the establishment of a modified version of the classic SILAC approach routinely used in quantitative mass spectrometry (MS) to assay relative changes in protein levels. In the newly-devised approach termed pulsed SILAC (pSILAC) differentially treated cells are transferred to culture medium supplemented with different versions of stable-isotope labeled heavy amino acids. As MS-based relative quantification is exclusively based on the newly-synthesized heavy protein amounts the method enables the detection of differences in protein production resulting from the treatment. The second part of the thesis shows the use of pSILAC to globally quantify the impact of microRNAs onto the proteome. Ectopic over-expression or knock-down of a single microRNA both affected protein production of hundreds of proteins. pSILAC identified several target genes as exclusively translationally regulated as changes in corresponding transcript levels were virtually absent. Measuring newly-synthesized protein amounts with heavy amino acids in a pulsed-labeling fashion has also been used to determine turnover rates of individual proteins, described in the third part of the present work. Along with transcript turnover as well as mRNA and protein levels they are essential for a dynamic description of gene expression. Simultaneous application of the nucleoside analogue 4-thiouridine (4sU) and heavy amino acids (SILAC) to metabolically label newly-produced mRNAs and proteins in mouse fibroblasts resulted in the calculation of mRNA and protein lifetimes and absolute levels for approximately 5,000 genes. While mRNA and protein levels were overall well correlated, a correlation between mRNA and protein half-lives was virtually absent. Yet this seemingly chaotic distribution of mRNA and protein half-lives was highly instructive since specific gene subsets have obviously evolved distinct combinations of half-lives that relate to their biological functions.
Van, Rooyen Marina. "The modulating effect of myo-inositol and other antidepressants on the mRNA levels and protein expression of selected subcellular enzymes / Marina van Rooyen." Thesis, North-West University, 2005. http://hdl.handle.net/10394/504.
Повний текст джерелаThesis (M.Sc. (Pharmacology))--North-West University, Potchefstroom Campus, 2005.
Peter, Martina Andrea. "Growth hormone-dependent expression and regulation of insulin-like growth factor (IGF) I and IGF binding protein mRNA levels in rat tissues in vivo /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10291.
Повний текст джерелаKogan, Cary. "The expression of neurofilament protein and mRNA levels in the lateral geniculate nucleus and area V1 of the developing and adult vervet monkey (Ceorcopithicus aethiops) /." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0028/MQ50807.pdf.
Повний текст джерелаBeyerle, Jolantha [Verfasser], and Heinz [Akademischer Betreuer] Schmeiser. "Assessment of mRNA, protein levels and activities of xenobiotic metabolizing enzymes in colon and rectal mucosa of colorectal cancer patients / Jolantha Beyerle ; Betreuer: Heinz Schmeiser." Heidelberg : Universitätsbibliothek Heidelberg, 2016. http://d-nb.info/1180736281/34.
Повний текст джерелаArslan, Sevki. "Effects Of Benzene On Liver, Kidney And Lung Cyp1a, Cyp2b4, Cyp2e1 And Cyp3a6 Mrna, Protein Level, And Drug Metabolizing Enzyme Activities And Toxicity In Diabetic Rabbits." Phd thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609446/index.pdf.
Повний текст джерелаBeresford, Guy William. "Control of glucokinase and mRNA levels in hepatocytes." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386292.
Повний текст джерелаAlqara, Yazan Ali. "Protein Interactions in mRNA Methylation Complexes." ScholarWorks@UNO, 2013. http://scholarworks.uno.edu/honors_theses/28.
Повний текст джерелаJones, David G. L. "Regulation of chick fibronectin mRNA levels by steroid hormones." Thesis, University of Manchester, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.277354.
Повний текст джерелаVail, Daniel Robert. "Artemisinin Biosynthesis: Developmental and Sugar Regulation of mRNA Levels." Digital WPI, 2008. https://digitalcommons.wpi.edu/etd-theses/415.
Повний текст джерелаWilson, Timothy Craig. "The role of mRNA stability and Fos protein in transient c-fos mRNA accumulation." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304567.
Повний текст джерелаChernov, Konstantin Grigorievich. "Interplay of YB-1 between tubulin and mRNA." Thesis, Evry-Val d'Essonne, 2008. http://www.theses.fr/2008EVRY0040/document.
Повний текст джерелаYB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs. We identify several novels YB-1 protein partners by affinity chromatography of different tissue extracts. We observed that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. Microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure where YB-1 probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Additionally, we demonstrated that tubulin interferes with mRNA:YB-1 complexes. These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation. The translational status of mRNPs in vivo depends on amount of YB-1 associated with mRNA. We show here that at low YB-1:mRNA ratios mRNP complexes possess an incompact structures, whereas saturated mRNPs are compact. This structural change corresponds to translation inhibition when mRNA moves from polysomal (translatable) to free (untranslatable) mRNPs. Saturated mRNPs bind to microtubules via protein:protein interactions and tend to self-aggregate on microtubule surface. This property could contribute to stress granule formation, mRNPs traffic and localization of translation apparatus within cytoplasm. Finally, the facilitated diffusion model was developed to explain enhancement of microtubule assembly by positively charged natural polyamines in living cells. Altogether our data contribute to the understanding of fundamental biological processes
Zhang, Jingji. "Accuracy of mRNA Translation in Bacterial Protein Synthesis." Doctoral thesis, Uppsala universitet, Struktur- och molekylärbiologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-262901.
Повний текст джерелаGlatz, Elisabeth. "Bacillus subtilis GlpP protein, antitermination and mRNA stability." Lund : Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/68945078.html.
Повний текст джерелаKarmouch, Jennifer. "Deciphering ColQ induced mechanisms in the control of AChR mRNA levels." Thesis, Paris 5, 2014. http://www.theses.fr/2014PA05T007/document.
Повний текст джерелаColQ is a specific collagen that anchors acetylcholinesterase (AChE) in the synaptic cleft of the neuromuscular junction (NMJ). The importance of AChE-ColQ complex in the physiology of this synapse has been highlighted by the identification of COLQ mutations in the human gene, leading to a congenital myasthenic syndrome (CMS) with AChE deficiency. The lack of AChE has been incriminated for the symptoms observed in patients along with NMJ defects in the CMS mouse model (ColQ-deficient). However, symptoms observed in the patients and mouse model of CMS with AChE deficiency are complex and AChE deficiency cannot account for all of them. We have demonstrated that ColQ could play a role per se in synapse formation which would explain some of the defects observed in patients and model mice. Indeed, we have shown that ColQ controls the clustering of Acetylcholine Receptors (AChR) and the expression of a number of specific synaptic genes. The most striking effect of the absence of ColQ is an upregulation of all AChR subunit mRNAs correlated by an increase in their protein levels. Preliminary results indicate that AChR mRNA upregulation is not transcriptional. This thesis deciphers the mechanisms that drive AChR mRNA upregulation in the absence of ColQ and the pathways that connect ColQ to the AChR RNA metabolism. Accordingly, we hypothesize that the absence of ColQ induces an upregulation of the stabilization of AChR subunit mRNAs, a post-transcriptional mechanism mediated by HuR. HuR is an RNA binding protein which stabilizes its target transcript by binding AU-rich elements (AREs) in their 3’UTR. HuR is critical during skeletal myogenesis and post-synaptic NMJ formation due to its stabilization of such transcripts as myogenin, MyoD and AChE. In this study, we show for the first time that a post-transcriptional mechanism of AChR mRNA stabilization is responsible for the ColQ mediated increase of AChR mRNAs. In support of these findings, the absence of ColQ also increased HuR mRNA and protein levels. We demonstrate that HuR is capable of binding to conserved ARE elements in the 3’UTR of AChR subunit mRNA. HuR’s interaction with AChR mRNA increased the stability of the transcripts, resulting in an increase in mRNA levels. Three major conclusions emerge from my thesis: we provide evidence that (1) in addition to transcriptional and assembly regulation of AChR, post-transcriptional mechanisms of AChR mRNA exist (2) ColQ regulates HuR mediated AChR stability through MuSK and (3) the p38 signalling pathway controls the levels of HuR in a ColQ dependent manner. Collectively, our data provides insight into the muscle signaling pathways which are affected by ColQ mutations leading to CMS with AChE deficiency. Thus, we have identified specific new molecular targets that may become important for the development of therapeutic interventions for patients with CMS
Spangler, Jacob Brian. "Conserved noncoding sequences regulate steady-state mRNA levels in Arabidopsis thaliana." Thesis, Clemson University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3565242.
Повний текст джерелаArabidopsis thaliana has undergone three whole genome duplications within its ancestry, and these events have dramatically affected its gene complement. Of the most recent whole genome duplication events (α event), there remain 11,452 conserved noncoding sequences (CNSs) that have been retained proximal to α duplicate gene pairs. As functional DNA elements are expected to diverge in sequence at a slower rate than nonfunctional DNA elements, the retained CNSs likely encode gene regulatory function. Within this dissertation I provide evidence for the regulatory role of CNSs within Arabidopsis thaliana. Using a collection of over 5,000 microarray RNA expression profiling datasets, I demonstrate that the presence of CNSs near α duplicate pairs is correlated with changes in average expression intensity (AEI), α duplicate pair co-expression, mRNA stability, and breadth of gene expression. The effects of CNSs on AEI, co-expression, and mRNA stability vary relative to their subgene position, because they are located in nontranscribed (5’-upstream and 3’-downstream) and transcribed (5’- UTR, intronic and 3’-UTR) regions. Modeling gene interactions through the generation of co-expression networks, I also demonstrate that a portion of CNSs participate in known gene regulatory networks. Collectively, this body of work demonstrates that CNSs regulate steady-state mRNA levels within Arabidopsis thailiana through both transcriptional and post-transcriptional mechanisms.
Smith, Paul Andrew. "Protein-protein interactions of Prp2p : a pre-mRNA splicing factor of Saccharomyces cerevisiae." Thesis, University of Edinburgh, 2000. http://hdl.handle.net/1842/12988.
Повний текст джерелаWüst, Michaela [Verfasser]. "The protein interactome of human SOD2 mRNA / Michaela Wüst." Tübingen : Universitätsbibliothek Tübingen, 2021. http://d-nb.info/1226756166/34.
Повний текст джерелаNaski, Nadia. "The p53, Mdm2 and Mdmx protein and mRNA interplay." Paris 7, 2010. http://www.theses.fr/2010PA077158.
Повний текст джерелаThe p53 transcription factor is studied Worldwide for its key fonction as a tumour suppressor protein. Importantly, the gene coding for p53 is mutated in around 50% of human cancers. However, malignant transformation can also occur in a p53 WT context, and this is believed to be caused by the abrogation of the regulatory pathways upstream or downstream of p53. Indeed, p53 is tightly regulated by aplethora of positive and negative regulatory factors. Mdm2 and its homolog Mdmx (also called Mdm4) are two major negative regulators of p53. They can bind to the p53 protein and induce respectively its degradation or its transrepression. In addition, under specific conditions, p53 can transactivate the mdm2 and mdmx genes, thus implicating the three proteins in negative feedback loops. Interestingly, our group has also shown recently that in addition to its inhibitory function, Mdm2 could positively regulate p53 by enhancing its mRNA translation. Here, we show that the capacity of Mdm2 to bind to the/?53 mRNA is independent of their protein-protein interaction. However, the conformation of the p53 mRNA seems to play a key role in its interaction with Mdm2. Moreover, since Mdm2 and Mdmx share a high structural homology, it was interesting to investigate whether Mdmx could play similar functions. Surprisingly, even though it also binds to the p53 mRNA, Mdmx, by its own, is unable to trigger p53 synthesis, contrary to Mdm2. However, we show that the p53 protein is able to bind to the Mdmx transcript (on its 5'UTR) and drastically inhibit its translation, under normal and genotoxic conditions. Altogether, these data add new feedback loops into the intricate p53 regulatory network
SALADINO, Patrizia. "ROLE OF RNA BINDING PROTEIN IN THE NERVE CELL DIFFERENTIATION." Doctoral thesis, Università degli Studi di Palermo, 2014. http://hdl.handle.net/10447/91214.
Повний текст джерелаUlbricht, Randi J. "Puf1p-mediated mRNA decay and combinatorial control of mRNA stability by the yeast Puf proteins." Diss., St. Louis, Mo. : University of Missouri--St. Louis, 2008. http://etd.umsl.edu/r2761.
Повний текст джерелаCass, Danielle Marie. "Role of two RNA binding properties in pre-mRNA splicing /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1400950811&sid=2&Fmt=2&clientId=11238&RQT=309&VName=PQD.
Повний текст джерелаTypescript. Includes vita and abstract. Includes bibliographical references (leaves 67-80). Also available for download via the World Wide Web; free to University of Oregon users.
Mitchell, Michael Robert. "Local Control of Translation and mRNA and Protein Decay is Sufficient for Protein Localization." Thesis, The University of Arizona, 2011. http://hdl.handle.net/10150/144847.
Повний текст джерелаPryor, Anne M. "Growth-regulated expression and G0-specific turnover of the mRNA that encodes AH49, a mammalian protein highly related to the mRNA export protein UAP56." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1069259604.
Повний текст джерелаTitle from first page of PDF file. Document formatted into pages; contains xii, 187 p.; also includes graphics (some col.) Includes bibliographical references (p. 175-187). Available online via OhioLINK's ETD Center
LU, Zhibo, Mayumi HOJO, Kenji YASUI, Itsuo KODAMA, and Kaichiro KAMIYA. "mRNA Levels of ERG, KVLQT1 and minK in Rabbit Right and Left Ventricles." Research Institute of Environmental Medicine, Nagoya University, 2002. http://hdl.handle.net/2237/2801.
Повний текст джерелаPettersson, Erik. "Investigation of tissue factor mRNA levels in human platelets using real-time PCR." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-180831.
Повний текст джерелаTanay, Véronique Anne-Marie Isabelle. "The action of antidepressant/antipanic drugs on GABA(A) receptor subunit mRNA levels." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0009/NQ34845.pdf.
Повний текст джерелаJagiełło, Bogumiła 1987. "Investigating protein complexes potentially governing microtubule-dependent MBP mRNA distribution." Doctoral thesis, Universitat Pompeu Fabra, 2018. http://hdl.handle.net/10803/665721.
Повний текст джерелаLocalization of mRNA molecules enables locally and temporally regulated protein synthesis, which is vital for many fundamental cellular processes. In oligodendrocytes transport of myelin basic protein (MBP) mRNA to myelinating processes allows for local synthesis of MBP. MBP has an essential function if forming the myelin sheath that surrounds axons, nurtures the nerve cells and allows for fast stimuli transmission. Despite extensive research about the molecular mechanisms that guide the transport and local expression of MBP-mRNA it is still not clear how this mRNA is transported to its destination and how activation of translation at the right place is controlled. Using the information from published data and an interaction screen performed in the lab we selected two protein complexes essential for MBP mRNA localisation and attempted to build them from bottom-up. In this approach in vitro reconstitution of the minimal complexes allows us to analyse the function of each component of the complex and understanding better how biological processes are regulated. MBP mRNA synthesis is partially dependent on the interaction between an RNA binding protein, hnRNPA2, and a microtubule polymerase, chTOG. To elucidate the mechanism by which this interaction triggers MBP mRNA translation we decided to analyse the functional consequences of this entirely novel interaction between a microtubule binding and RNA binding protein. I successfully produced functional recombinant hnRNPA2 and chTOG. The interaction detected between those two proteins turned out to be unspecific. I hypothesize it is due to the conformation that hnRNPA2 acquires in the RNP granule or depends on other components. Due to the lack of resources to investigate this further we suspended this project. Delivery of MBP mRNA to the oligodendrocytes processes is maintained by microtubule-dependent kinesin motors. The kinesin Kif1Bβ has been shown to carry out the transport but the adaptors, which link the mRNA to the motor, remain unknown. Kif1Bβ is known to be responsible for transport of membranous organelles such as synaptic vesicle (SV) precursors. Evidence from literature and protein interaction screen performed in the lab suggests that Kif1Bβ could achieve mRNA transport by vesicle hitchhiking. The regulation of the kinesin is not fully understood therefore I decided to investigate this aspect first. For this reason I sought to build and characterize a Kif1Bβ-dependent vesicle transport system in vitro. I produced recombinant protein and achieved characterization of the Kif1Bβ motor. Kif1Bβ is able to independently form a dimer in solution and in the dimeric state processively moves on microtubules. I detected the preference of Kif1Bβ for certain lipids, which were then used to produce synthetic vesicles. In in vitro motility assays I characterized the Kif1Bβ-driven transport of synthetic vesicles. The reconstitution of the interactions between proteins involved in vesicle transporting complex proved to be more problematic. Additional efforts are needed to achieve this goal and further characterize the proposed complex in the future. Despite my efforts I did not manage to fully reconstitute any of the two complexes of interest. However, I report in this thesis a detailed description of the approaches and the partial complexes I managed to build and characterise.
Stojdl, David F. "Protein kinases and the regulation of mRNA splicing and translation." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0016/NQ58294.pdf.
Повний текст джерелаPersdotter, Hedlund Gabriella. "Protein and mRNA Studies of Rat FA1/Pref-1/dlk." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7773.
Повний текст джерелаKinney, Emma. "Decoupling of HSV1 Vhs protein mRNA decay and translation stimulation." Thesis, University of Missouri - Kansas City, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=1543940.
Повний текст джерелаHerpes Simplex Virus Type 1 is a member of the alphaherpesvirinae subfamily within the family Herpesviridae. This virus has both a lytic and latent cycle. Primary infection occurs when the virus enters epithelial cells around the mucosal lining of the nose and mouth. Within the epithelial cells, the virus undergoes an active lytic infection, causing an ulcerated blister, more famously known as a 'cold sore' or 'fever blister'. Once HSV enters the nearby sensory neurons the genome is transported to the neuronal cell body where its latency associated transcripts are activated and the virus remains in a dormant latent cycle until reactivation, when the virus is transported back down the axon to the epithelial cells at or near the site of initial infection. The Virion Host Shutoff protein is a tegument protein from HSV1 and acts as a ribonuclease, degrading both cellular and viral mRNAs, making the course of viral infection more efficient. A study by Saffran, Read and Smiley uncovered an unexpected new function of Vhs: stimulation of translation from some IRESs. An IRES is a section of mRNA with a high level of secondary structure, capable of inducing cap-independent translation. In similar experiments utilizing a bicistronic reporter transcript, I sought to discover whether or not these two functions of the Vhs protein could be de-coupled. Experiments involved dually transfecting HeLa cells with different Vhs mutants across a range of Vhs plasmid concentrations and the bicistronic reporter construct. Levels of reporter activity were measured from cell lysates 36 hours after transfections and provided a measurement of the control at the level of translation. As the cellular Bip IRES element was present between the cistrons, the 3' cistron provided a measure of IRES stimulation. The Results revealed examples of Vhs mutants in which the two activities had been separated. It is unknown what role IRES stimulation could play during Herpesvirus infection, although it is interesting to note that some HSV1 genes have IRES like elements within the 5' UTR. Future experiments can be done to investigate whether or not Vhs is actively recruiting transcription initiation factors to these IRES elements.
Lemay, Guy. "Study of a reovirus protein involved in viral mRNA translation." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75419.
Повний текст джерелаBelmont, Brian J. (Brian Joshua). "Engineered mRNA regulation using an inducible protein-RNA aptamer interaction." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/78136.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (p. 114-131).
The importance and pervasiveness of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is appealing given the potential to subsequently modulate RNA biology in a manner decoupled from normal cellular physiology. We describe a ligand-responsive protein-RNA interaction module that can be used to target a specific RNA for subsequent regulation. Using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method, RNA aptamers binding to the bacterial Tet Repressor protein (TetR) with low- to sub- nanomolar affinities were identified. This interaction is reversibly controlled by tetracycline in a manner analogous to the interaction of TetR with its cognate DNA operator. Aptamer minimization and mutational analyses support a functional role for conserved sequence and structural motifs in TetR binding. We illustrate the utility of this chemically-inducible RNA-protein interaction to directly regulate translation in both a prokaryotic and eukaryotic organism. By genetically encoding TetR-binding RNA elements into the 5'-untranslated region (5'-UTR) of a given mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5'-UTR contexts, this modular system efficiently regulates the expression of multiple target genes, and is sufficiently stringent to distinguish functional from nonfunctional RNA-TetR interactions. We also demonstrate engineering this TetR-aptamer module to regulate subcellular mRNA localization. This is efficiently achieved by fusing TetR to proteins natively involved in localizing endogenous transcripts, and genetically encoding TetR-binding RNA aptamers into the target transcript. Using this platform, we achieve tetracycline-regulated enhancement of target transcript subcellular localization. We also systematically examine some rules for successfully forward engineering this RNA localization system. Altogether, these results define and validate an inducible protein-RNA interaction module that incorporates desirable aspects of a ubiquitous mechanism for regulating RNA function in Nature and that can be used as a foundation for functionally and reversibly controlling multiple fates of RNA in cells.
by Brian J. Belmont.
Ph.D.
Bourdeau-Julien, Isabelle. "ALS-associated RNA-binding protein FUS and mRNA translation regulation." Master's thesis, Université Laval, 2020. http://hdl.handle.net/20.500.11794/68742.
Повний текст джерелаMutations in several genes have been linked to amyotrophic lateral sclerosis (ALS),particularly in the gene coding for the Fused in Sarcoma protein (FUS). Those mutations are found in the part encoding for the nuclear localization signal, making the protein abnormallyabundant in the cytoplasm. Combined with other observations, it suggests that a toxic gainof function of FUS in the cytoplasm would be the cause of the neurodegeneration. ALS is a neurodegenerative disease that affects motor neurons and causes progressive paralysis. The molecular mechanisms causing the disease are still unknown. One of the hypotheses is the disruption of local translation of mRNAs, which allows synapses to respond quickly and independently from the cell body. Insufficient local translation to support long-term synapticactivity would lead to synaptic loss and neurodegeneration. Thereby, the objective of mystudy is to determine the role of FUS in the regulation of mRNA translation by characterizing its interaction with translational components and evaluate its function in an ALS-linked condition. I have shown that FUS is associated with stalled polyribosomes, which suggests that it plays a role in regulating mRNA translation by interacting with the core of translation.There is also an increase in the presence of FUS in the cytoplasm and in its interaction with polyribosomes following inhibition of translation through mTOR, suggesting its role as anegative regulator. In addition, ALS-related mutations amplify FUS inhibitory function bymaking FUS cytoplasmic and reducing protein synthesis. My results show that the FUSprotein would have a role as a translation inhibitor when it is cytoplasmic. There fore, increasing the presence of FUS in the cytoplasm in ALS would result in significant translation inhibition, at a level insufficient to support synaptic activity.
Thumdee, Patama. "The prenatal expression of mRNA and protein of the prion protein gene, PRNP, in sheep." [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=983755728.
Повний текст джерелаWang, Jingwei. "Alterations in protein kinase A and protein kinase C activities and protein levels in cardiomyopathy." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0001/MQ32280.pdf.
Повний текст джерелаLe, Cras Timothy David. "Regulation of the levels of mRNA for the LDL receptor and HMG CoA reductase." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239497.
Повний текст джерелаHenscheid, Kristy L. "Functional conservation and RNA binding of the pre-mRNA splicing factor U2AF65 /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1400950821&sid=5&Fmt=2&clientId=11238&RQT=309&VName=PQD.
Повний текст джерелаTypescript. Includes vita and abstract. Includes bibliographical references (leaves 129-141). Also available for download via the World Wide Web; free to University of Oregon users.
Frey, Steffen. "Charakterisierung von Scp160p, einem mRNA- und ribosomenassoziierten Protein in Saccharomyces cerevisiae." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=966002172.
Повний текст джерелаSingh, Mamata. "Insights into the Renal Protective Mechanisms of mRNA Binding Protein HuR." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1300995188.
Повний текст джерелаTurk, Casey M. "Paralemmin splice variants and mRNA and protein expression in breast cancers." Connect to this title, 2008. http://scholarworks.umass.edu/theses/194/.
Повний текст джерелаFernandes, Pereira Carina. "The influenza A virus NS1 protein and viral mRNA nuclear export." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/275570.
Повний текст джерелаGuzella, Thiago dos Santos. "Variation in protein expression levels in cell populations." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2013. http://hdl.handle.net/10362/11968.
Повний текст джерелаA widespread observation is that of variation in the expression levels of a molecule when analyzing a snapshot of single cells from a population. One possible explanation for this variation is that there are asynchronous fluctuations throughout time in the expression level of each cell, for example due to noise in gene expression. Another explanation is that there are so-called stable variants, groups of cells that have a permanent bias for a limited range of expression levels compared with the levels observed in a snapshot of the population. In an extreme scenario, each cell of the population would be a single stable variant with an expression level that is constant as a function of time, without any fluctuations. Stable variants could occur if the population is genetically diverse, with each clone being associated with a particular expression level.(...)
Chen, Jinyun. "REGULATION OF INTRACELLULAR ARYL HYDROCARBON RECEPTOR PROTEIN LEVELS." Scholarly Commons, 2020. https://scholarlycommons.pacific.edu/uop_etds/3675.
Повний текст джерелаOhlmann, Theophile. "The role of eIF4F in IRES-driven and uncapped mRNA translation." Thesis, University of Sussex, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336325.
Повний текст джерелаSampson, Natalie D. "Identification and functional characterisation of the novel pre-mRNA processing factor SCAF6." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272374.
Повний текст джерелаWang, Weizhong. "Nuclear galectins and their role in pre-mRNA splicing." Diss., Connect to online resource - MSU authorized users, 2006.
Знайти повний текст джерелаTitle from PDF t.p. (viewed on Nov. 20, 2008) Includes bibliographical references. Also issued in print.