Дисертації з теми "Proline-rich"
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Dikic, Inga. "Signal Transduction by Proline-Rich Tyrosine Kinase Pyk2." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2002. http://publications.uu.se/theses/91-554-5316-3/.
Повний текст джерелаBess, Kirstin. "Transcriptional repression by the proline rich homeodomain protein." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390994.
Повний текст джерелаMurray, Nicola Jane. "NMR studies of salivary proline-rich proteins and tannins." Thesis, University of Sheffield, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284595.
Повний текст джерелаSwingler, Tracey. "Transcriptional repression by the proline rich homeodomain protein (PRH)." Thesis, University of Bristol, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419215.
Повний текст джерелаDrzymala, Luke. "Phosphorylation of human salivary proline-rich proteins in cultured cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0001/MQ40692.pdf.
Повний текст джерелаSoufi, Abdenour. "Oligomerisation and phosphorylation of the proline-rich homeodomain protein (PRH)." Thesis, University of Bristol, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432944.
Повний текст джерелаChan, Maggie Tin Lai. "Proteolytic processing of recombinant human salivary proline-rich protein precursors (PRPs)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0025/MQ50333.pdf.
Повний текст джерелаKofler, Michael. "GYF domains a class of proline rich ligand binding adaptor domains /." kostenfrei, 2007. http://www.diss.fu-berlin.de/2007/261/index.html.
Повний текст джерелаLu, Ying. "Characterization of the interaction of human salivary proline-rich proteins with tannins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq29245.pdf.
Повний текст джерелаCharlton, Adrian Jon. "Study of the interaction between salivary proline-rich proteins and plant polyphenols." Thesis, University of Sheffield, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327666.
Повний текст джерелаNoy, Peter John. "Coordinate regulation of Vegf signalling genes by the proline rich homeodomain protein." Thesis, University of Birmingham, 2011. http://etheses.bham.ac.uk//id/eprint/1527/.
Повний текст джерелаKan, Su-Yin. "The proline-rich repeat and thioester domains of streptococcal fibronectin-binding proteins." Thesis, University of St Andrews, 2014. http://hdl.handle.net/10023/6134.
Повний текст джерелаShiban, Ehab [Verfasser]. "Characterization of the Proline-rich Synapse-associated Protein 1 Knockout Mouse / Ehab Shiban." Ulm : Universität Ulm. Medizinische Fakultät, 2013. http://d-nb.info/1035802562/34.
Повний текст джерелаGreenwood, Michael T. "Isolation and characterization of Dictyostelium discoideum genes encoding a common proline-rich region." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=39517.
Повний текст джерелаFerraz, Junior Jose Candido de Souza. "Prime-boost vaccination strategies using the Mycobacterium tuberculosis APA (alanine-proline rich antigen)." Thesis, University College London (University of London), 2004. http://discovery.ucl.ac.uk/1446909/.
Повний текст джерелаAlaoui-Ismaili, Moulay Hicham. "Roles of three proline-rich proteins at late stages of entomopoxvirus and baculovirus morphogenesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0016/NQ55295.pdf.
Повний текст джерелаWilliams, Hannah. "Investigating the DNA binding properties of the proline rich homeodomain, an oligomeric transcription factor." Thesis, University of Bristol, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503856.
Повний текст джерелаGeng, Wei, and 耿瑋. "The role of proline rich tyrosine kinase 2 (Pyk2) on cisplatin resistance in HCC." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43224106.
Повний текст джерелаGeng, Wei. "The role of proline rich tyrosine kinase 2 (Pyk2) on cisplatin resistance in HCC." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43224106.
Повний текст джерелаBIGI, ALESSANDRA. "Characterization of human sialidase NEU4: role of the proline-rich region in signal transduction." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2011. http://hdl.handle.net/10281/19197.
Повний текст джерелаWu, Yih-Yiing. "Response of skin to noxious stimuli : studies using in situ hybridisation." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263124.
Повний текст джерела実里, 岩田, and Minori Iwata. "Study of mechanisms for the axonal localization of the tau protein in neurons." Thesis, https://doors.doshisha.ac.jp/opac/opac_link/bibid/BB13142995/?lang=0, 2020. https://doors.doshisha.ac.jp/opac/opac_link/bibid/BB13142995/?lang=0.
Повний текст джерелаMicrotubule-associated protein tau localizes specifically to neuronal axons. In order to elucidate the molecular mechanism of the axon localization of tau, we constructed an expression system for axon specific localization of exogenous tau in immature neurons in culture. Using this system, it suggested that the proline rich region 2 (PRR2) and phosphorylation of PRR2, which contains important phosphorylation sites, is critical for the localization. In the future, this experimental system will contribute greatly to the study of tau in normal and in the pathology of Alzheimer's disease.
博士(理学)
Doctor of Philosophy in Science
同志社大学
Doshisha University
Sun, Kin-wai, and 孫建維. "The significance of proline rich tyrosine kinase 2 (PYK2) in proliferation and invasiveness of hepatocellular carcinoma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B40687375.
Повний текст джерелаSun, Kin-wai. "The significance of proline rich tyrosine kinase 2 (PYK2) in proliferation and invasiveness of hepatocellular carcinoma." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B40687375.
Повний текст джерелаSawasdichai, Anyaporn. "The regulation of cell growth by the proline rich homeodomain protein and its importance in human diseases." Thesis, University of Bristol, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546220.
Повний текст джерелаFaure, Camille. "Régulation de la localisation et de l'activation de la tyrosine kinase Pyk2 (Proline-rich tyrosine kinase 2)." Paris 6, 2010. http://www.theses.fr/2009PA066736.
Повний текст джерелаYamasaki, Masahide. "Monocyte chemoattractant protein-1 causes differential proline-rich tyrosine kinase 2-mediated signaling in THP-1 cells." Kyoto University, 2001. http://hdl.handle.net/2433/150583.
Повний текст джерелаMok, Ka Wai. "Proline-rich membrane anchor (PRiMA) of acetylcholinesterase (AChE) : characterization of its splicing variants and their expression profiles in different chicken tissues /." View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202009%20MOK.
Повний текст джерелаCharlton, Bernard. "Cloning and characterization of Drosophila Nedd4 as a putative binding partner of the proline-rich region of Inscuteable." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0004/MQ45499.pdf.
Повний текст джерелаRoberts, Daniel Stephen. "The role of the proline rich homeodomain in the regulation of proliferation, survival and migration of breast cells." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/4873/.
Повний текст джерелаDonnelly, Sean Frederick Hilton. "Isolation and characterisation of a gene encoding verprolin, a novel proline-rich protein in the yeast Saccharomyces cerevisiae." Thesis, University of Leicester, 1993. http://hdl.handle.net/2381/34405.
Повний текст джерелаFowler, Thomas James. "Characterization of four proline-rich protein genes in Arabidopsis and identificaiton of a defective kernel mutant in maize /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487847761307916.
Повний текст джерелаWang, Shu-Zhen. "Isolation and characterization of the messenger RNA and the gene coding for a proline-rich zein from corn endosperm." Diss., Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/49959.
Повний текст джерелаPh. D.
incomplete_metadata
Mack, Birgit H. "Identification of proline-rich motifs in human WIP that mediate a conserved interaction with the yeast Hof1p SH3 domain." Thesis, Griffith University, 2020. http://hdl.handle.net/10072/391078.
Повний текст джерелаThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Medical Science
Griffith Health
Full Text
Xie, Qunhui. "Transcriptional regulation and function of PRiMA (proline-rich membrane anchor), a membrane anchor of globular acetylcholinesterase, in muscle and neuron /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202006%20XIE.
Повний текст джерелаSavastano, Adriana [Verfasser]. "Investigation on the Physiological and Pathological Aspects of the Proline-Rich Region of the Microtubule-Associated Protein Tau / Adriana Savastano." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1223706311/34.
Повний текст джерелаHosono, Yuji. "Splicing factor proline/glutamine-rich is a novel autoantigen of dermatomyositis and associated with anti-melanoma differentiation-associated gene 5 antibody." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225498.
Повний текст джерелаBOROUMAND, MOZHGAN. "Extensive proteomics characterization of basic proline-rich proteins in human saliva and investigation on their properties as substrates of epithelial transglutaminase 2." Doctoral thesis, Università degli Studi di Cagliari, 2019. http://hdl.handle.net/11584/260381.
Повний текст джерелаFaurie, Benoit. "Les sucres et l'astringence : effet des polysaccharides présents dans le vin sur les interactions tanins-protéines." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0191/document.
Повний текст джерелаTannins play a key role in the organoleptic qualities of red wine. They are responsible for wine astringency, a dry, rought and puker sensation perceived in the mouth while tasting. This sensation is the consequence of a specific interaction between tannins and saliva proteins, mainly Proline Rich Protein (PRPs). The first part of this work was to study the influence of various sugars on the self-association of tannins process as well as tannins - proteins interactions. The colloidal behavior of a tannin (the epigallocatechin gallate - EGCG), as well as its interaction with a representative peptide IB9-14 of PRPs was studied in the presence of various simple sugars and polysaccharides. The parameters of the interaction were determined for all systems, highlighting the existence of an interaction between EGCG and sugars whose affinity seems to depend on the sugar polymerization degree. This interaction does not interfere, under the experimental conditions tested, on the association between tannins and peptide. The second part of this work was to realize the full synthesis of the protein IB9 including the peptidic sequence of IB9-14, and to study its interaction with two procyanidins: EGCG and dimer B3. The results show and confirm the influence of the length of the peptide chain interactions with tannins
ORRU', ROBERTO. "Sequenziamento e Analisi Molecolare di Varianti Alleliche del Gene PRB1." Doctoral thesis, Università degli Studi di Cagliari, 2016. http://hdl.handle.net/11584/266626.
Повний текст джерелаPelillo, Chiara. "Therapeutic potential of BAC7(I-35), a Proline-rich Antimicrobial Peptide: in vitro and in vivo studies and Pegylation strategy to improve its bioavailability." Doctoral thesis, Università degli studi di Trieste, 2011. http://hdl.handle.net/10077/5978.
Повний текст джерелаThe antimicrobial peptides (AMPs) are an important component of the innate defense against invading microorganisms, are widespread in nature and may have multiple and diversified mechanisms of bactericidal action. In addition to their direct antimicrobial activity the are also involved in other biological processes. The aim of this project was to investigate the in vivo activity of Bac7(1-35), a bovine proline-rich antimicrobial peptide, having in mind its possible use as a lead compound for the development of novel anti-infective agents. Before moving to animal models of infection, the in vitro stability of the peptide in the presence of murine and human serum or plasma as well as its biodistribution in mouse were investigated. Antibacterial activity assays against Salmonella enterica showed that the presence of murine blood components largely inhibits the antibacterial activity of the peptide. On the contrary, in human serum and plasma Bac7(1-35) maintains its efficacy. This is due to the more rapid degradation by proteases of murine blood. The in vivo biodistribution of Bac7(1-35) was investigated by using a time-domain optical imaging apparatus and a fluorescently-labeled Bac7(1-35) derivative. The compound reaches the kidney and the bladder respectively 1 and 3 hours after i.p. injection. The in vivo and ex vivo analyses performed after 24 h confirm that the compound has been totally excreted. A mouse model of S. typhimurium infection was set up and used to test the therapeutic efficacy of Bac7(1-35). Treatment of infected mice with the peptide injected i.p. immediately after a lethal, intraperithoneal bacterial challenge, increased the mean survival time and reduced significantly the number of viable bacterial cells in liver and spleen of treated mice at 3 days post-inoculum. In 1/3 of the organ homogenates, the bacterial presence was undetectable and this result matches the percentage of cured animals (35%). In an attempt to improve its pharmacokinetic profile, the peptide was conjugated with polyethylene glycol (PEG), a non-toxic, non-immunogenic and FDA-approved polymer. Different strategies of pegylation have been considered to find the best method in terms of chemical yield and of maintenance of biological activity. Pegylation via a thioether ligation resulted the best strategy to obtain a slow active peptide release in human blood components with a reduced renal clearance and an increased bioavailability of Bac7(1-35), as biodistribution analyses demonstrated. Several important pathogens, such as S. enterica, cause disease by surviving and replicating within host cells. Since many AMPs have also immunomodulatory activities, we investigated the effect of Bac7(1-35) on the interaction between macrophages and Salmonella. We carried out phagocytosis assays with macrophages and the results suggest that Bac7(1-35) plays a positive modulatory effect on this function. Phagocytosis assays were also performed to determine if Bac7(1-35) could inhibit survival and replication of intracellular Salmonella. The results show that the peptide inhibits the replication of intracellular Salmonella, suggesting that it can exert its antibacterial activity within eukaryotic cells. Further studies are required to fully understand the details of the Bac7(1-35) biological activities. The results obtained provide encouraging evidence for future investigations on Bac7(1-35) and on the pegylated form Bac7(1-35)CAM-PEG20k also in other models of infection and with different intracellular pathogens.
XXIII Ciclo
1981
Liu, Xiao. "Bioinformatic Identification and Analysis of Hydroxyproline-rich Glycoproteins in Plants." Ohio University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou150037001088989.
Повний текст джерелаCanon, Francis. "Contribution de la spectrométrie de masse à l'étude des interactions entre les protéines salivaires riche en proline et les tanins." Thesis, Montpellier, SupAgro, 2010. http://www.theses.fr/2010NSAM0015/document.
Повний текст джерелаAstringency is an important organoleptic property of plant-based food. It is attributed to interactions of tannins, which are polyphenolic compounds, with salivary proteins and especially proline rich proteins (PRPs), which belong to the group of intrinsically unstructured protein (IUP). Tannins play an important part in plant defence mechanisms. Indeed, they have an antinutritional effect as they inhibit digestive enzymes. Production of salivary PRP is thus an adaptation process to tannin-rich diets. The purpose of this work is to provide a closer look at PRPtannin supramolecular edifices in solution, using a mass spectrometry (MS) approach. The human salivary proteins IB5, a basic PRP, and II-1, a glycosylated PRP, have been produced by heterologous expression. After purification, both proteins have been characterized by MS using electrospray (ESI) and Matrix-Assisted Laser Desorption/Ionisation (MALDI) sources. The study of the interaction between IB5 and model tannins by ESI-MS confirmed the presence of IB5tannin non covalent complexes in solution and provided new information on their stoichiometries. Competitive interaction experiments between IB5 and two tannins, along with IB5tannin complexes dissociation studies revealed the impact of the main tannin chemical features on this interaction. Structural studies performed on IB5tanin edifices by Collision induced dissociation (CID), Electron Capture Dissociation (ECD) and photodissociation MS/MS experiments and by ion mobility coupled with MS showed the presence of several interaction sites on IB5 and conformational changes arising from the interaction
Weiler, Mary Claire. "Identification of the SH3 binding domains and the in vitro map kinase phosphorylation sites of CR16, a novel proline-rich protein expressed in Rat Brain neurons /." The Ohio State University, 1998. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487951214938305.
Повний текст джерелаFleau, Charlotte. "Maladie d'Alzheimer et polyphénols : effet des tanins sur la phosphorylation de la protéine Tau." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0776/document.
Повний текст джерелаHyperphosphorylation of Tau protein, which leads to their abnormal aggregation into neurofibrillary tangles and to neuronal loss observed in patients with Alzheimer disease, is regulated by a disequilibrium between kinases and phosphatases activities. This post traductional modification affects several residues, in particular in the Proline Rich Region of Tau (PRR). But, NMR studies, realized in our laboratory, have shown that polyphenols are able to interact with peptide Tau models issued from the PRR and the affinity are in the same range that those described for the kinases. In this context, we have envisaged to synthetize several polyphenols and to develop a phosphorylation reaction of peptide models in order to study, by mass spectrometry, the ability of these compounds to protect Tau against kinase attack
Palm-Forster, Mieder Anthony Thomas [Verfasser], Dierk [Akademischer Betreuer] Scheel, Sacha [Akademischer Betreuer] Baginsky, and Wolfgang [Akademischer Betreuer] Dröge-Laser. "Identification and functional characterisation of three novel Proline Rich Proteins that are Mitogen Activated Protein Kinase substrates / Mieder Anthony Thomas Palm-Forster. Betreuer: Dierk Scheel ; Sacha Baginsky ; Wolfgang Dröge-Laser." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2013. http://d-nb.info/1031915028/34.
Повний текст джерелаGehrke, Janin. "Untersuchungen zu tanninbindenden Speichelproteinen des Rehs und anderer Wiederkäuer." Phd thesis, Universität Potsdam, 2002. http://opus.kobv.de/ubp/volltexte/2005/42/.
Повний текст джерелаEinige Wiederkäuerarten wie z.B. das Reh (Capreolus capreolus) haben in ihrem Nahrungsspektrum viele stark tanninhaltige Pflanzen, leiden aber nicht unter den erwähnten postdigestiven Konsequenzen. Eine Möglichkeit, die antinutritive Wirkung von Tanninen zu neutralisieren, besteht in der Produktion tanninbindender Speichelproteine.
Der Speichel verschiedener Wiederkäuerarten wurde auf das Vorhandensein tanninbindender Proteine untersucht. Diese Arten wurden so ausgewählt, dass alle drei Ernährungstypen (Konzentratselektierer, Intermediärtyp, Gras- und Rauhfutterfresser) in den Vergleich eingeschlossen werden konnten. Als Referenzspezies wurde der Konzentratselektierer Reh herangezogen.
Die Speichelproteine des Rehs und die der Intermediärtypen (Rentier, Rangifer tarandus; Damhirsch, Cervus dama; Moschusochse, Ovibos moschatus) banden ungefähr doppelt so effektiv an hydrolysierbare Tannine (Tanninsäure), wie die der untersuchten Gras- und Rauhfutterfresser (Rind, Bos taurus; und Mufflon, Ovis orientalis musimon). Diese Abstufung zeigte sich auch bei der Untersuchung der Bindung an kondensierte Tannine (Quebracho). Eine Ausnahme stellte Mufflonspeichel dar, dieser band ebenso gut an Quebracho wie die Speichelproteine der anderen Ernährungstypen.
Über eine Aminosäuretotalanalyse konnte festgestellt werden, dass der Speichel einiger untersuchter Wiederkäuerarten prolinreiche Proteine (PRPs) enthielt. Unter Ausnutzung ihrer Trichloressigsäure (TCA)-Löslichkeit wurden diese angereichert und genauer untersucht. Die Analyse der TCA-löslichen Speichelproteine der Konzentratselektierer (Reh, Elch) ergab einen relativen Prolingehalt von über 35 %, während beim Moschusochsen noch 29 % gemessen wurden. In Damhirsch- und Rinderspeichel wurden keine prolinreichen Proteine gefunden.
Für die TCA-löslichen Speichelproteine des Rehs konnte eine hohe Tanninbindungskapazität nachgewiesen werden. Diese banden 24 - 30 x effektiver an Tannine als die TCA-löslichen Speichelproteine des Rindes. Die Tanninbindungskapazitäten der TCA-löslichen Speichelproteine von Moschusochse und Damhirsch waren ebenfalls höher als die des Rindes, aber niedriger als die des Rehs.
Die Kohlenhydrat-Analyse der TCA-löslichen Speichelproteine des Rehs erbrachte, dass es sich bei ihnen um Glykoproteine handelt. Mittels Gelfiltration und zweidimensionaler Polyacrylamidgelektrophorese konnten fünf Proteingruppen mit Molekulargewichten zwischen 15 und 50 kd sowie isoelektrischen Punkten zwischen 4,0 und 8,2 detektiert werden.
Von 15 dieser Proteine konnten die N-terminalen Aminosäuresequenzen ermittelt werden. Ausgehend von diesen Informationen wurden Reh-PRP spezifische mRNAs isoliert und partiell sequenziert. Die meisten dieser Fragmente hatten eine gemeinsame 18 Aminosäuren lange C-terminale Sequenz PPPEEQPEE/QSPDEE/DSPSE.
Die Suche nach Übereinstimmungen der analysierten Sequenzen mit anderen Säugetier-PRPs in der Genbank ergab keine sinnvollen Ähnlichkeiten. Die Ergebnisse können zu Informationen über tanninbindende Proteine anderer Wiederkäuer führen. Die Sequenzinformationen stellen einen Ausgangspunkt bei der Analyse der evolutiven Zusammenhänge der Cerviden dar.
Investigation of tannin binding salivary proteins of roe deer and other ruminants:
In this work the adaptation of herbivores to plant secondary metabolites was investigated with help of biochemical and molecular biological methods. In unadapted species plant secondary metabolites as tannins can reduce food digestibility and thus diminish growth rate and health status (antinutritive action). Tannins act through its astringency, that means the high capacity to bind proteins, other macromolecules and metal ions. Some ruminant species feed on tannin containing plant but do not suffer from the mentioned nutritive consequences. The production of tannin binding proteins is one possible adaptation mechanism to neutralize the effects of the tannins.
Saliva of six different ruminant species was investigated for the presence of tannin binding proteins. All three feeding types (concentrate selector, intermediate type and grass and roughage eater) were included in the comparison.
Salivary proteins from roe deer (Capreolus capreolus, concentrate selector) and from the intermediate feeding types (rein deer, Rangifer tarandus; fallow deer, Cervus dama; musk ox, Ovibos moschatus) bound twice as effective to hydrolysable tannins (tannic acid) as those from the investigated grass and roughage eaters (cattle, Bos taurus; moufflon, Ovis orientalis). This differentiation could also be observed investigating the binding capacities to condensed tannins (quebracho) except for moufflon. Moufflon salivary proteins bound with the same intensity to quebracho as the salivary proteins from the other feeding types.
Proline rich proteins (PRPs) could be accumulated from roe deer, moose and musk ox saliva by use of its solubility properties in 5 % trichloro acetic acid (TCA). Roe deer and moose TCA soluble salivary proteins contained more than 35 %, musk ox proteins 29 % proline. In fallow deer and cattle saliva PRPs could not be detected.
A tannin binding assay demonstrated for the TCA soluble salivary proteins from roe deer, musk ox and fallow deer but not from cattle, that they are able to bind tannins. Roe deer salivary proteins bound 24 to 30 more effective to tannins as cattle proteins. Tannin binding capacity of the proteins from musk ox and fallow deer saliva was higher as those from cattle but lower as those from roe deer.
For further analysis of ruminant tannin binding proteins we chose roe deer as reference species. Carbohydrate analysis of TCA soluble proteins from roe deer saliva showed that they were glycoproteins. With help of gel filtration and two dimensional polyacrylamid gel electrophoresis five proteins groups with molecular weights from 15 to 50 kd and isoelectric points from 4.0 to 8.2 could be detected.
N-terminal amino acid sequences of 15 of the roe deer salivary TCA soluble proteins were determined by Edmann degradation. This information led to partially sequenced roe deer PRP specific cDNA. An 18 amino acid long C-terminal sequence was common in most of the clones. The obtained roe deer PRP sequences did not match with known mammalian PRP sequences from data banks.
The finding in this work can lead to information about salivary tannin binding proteins in other ruminants. The sequence information represent a starting-point for the investigation of cervid evolution.
Paulhe, Frédérique. "Régulation différentielle du métabolisme des phosphoinositides par les intégrines αvβ3 et αvβ5 lors de la migration des cellules musculaires lisses". Paris 7, 2002. http://www.theses.fr/2002PA077140.
Повний текст джерелаMardirossian, Mario. "Internal targets and killing mechanism of the cathelicidin Bac7 in Gram-negative bacteria." Doctoral thesis, Università degli studi di Trieste, 2013. http://hdl.handle.net/10077/8640.
Повний текст джерелаBac7(1-35) is the smallest fragment of the proline-rich cathelicidin Bac7 that shows the same antibacterial activity as the whole natural peptide of 60 residues. In this work, we remarked that the unique gene whose deletion can confer resistance to E. coli against Bac7(1-35) is sbmA, coding for an inner membrane protein involved in the penetration of this peptide into bacterial cells. Moreover, we provided evidence that SbmA is also involved in the transmembrane transport of a fragment of another proline-rich antimicrobial peptide, arasin1(1-23), isolated from the spider crab. These findings suggest a general role of this membrane protein in the uptake of proline-rich antimicrobial peptides (PR-AMPs) into Gram-negative bacteria. We then measured the intrabacterial concentration reached by Bac7(1-35) in E. coli, and observed that this increases from micromolar in the medium to millimolar within the bacterial cell, suggesting that it may bind to cytosolic structures. For this reason, we looked for possible interactions between Bac7(1-35) and macromolecules involved in viable processes of bacteria. These studies showed that Bac7(1-35) completely inhibits in vitro the transcription/translation process starting from a concentration of 50 μM. Then we demonstrated that inhibition is: i) specific for Bac7(1-35), since it is not exerted by other cathelicidin-derived AMPs not belonging to the Prorich group, and ii) not stereo-specific, since it is exerted at the same level by the all-D isomer of Bac7(1-35). We also demonstrated the ability of Bac7(1-35) to bind DNA in vitro, but we excluded that this binding may represent the primary mechanism of bactericidal action. We also showed that the peptide does not significantly affect in vitro the transcription process, deducing that the inhibition of the transcription/translation targets primarily the translation process. We verified these data in vivo on E. coli cells measuring the incorporation of radioactive precursors of bacterial macromolecules. We observed that the exposure of bacteria to Bac7(1-35) inhibited the incorporation of radioactive leucine, but not of radioactive thymidine and uridine, indicating a specific block at the protein synthesis level and not of DNA and RNA synthesis. In the near future, a clearer definition of the intrabacterial target(s) of Bac7(1-35) would hopefully lead to the experimentation of this molecule or of its derivatives as a new generation antibiotic drug.
Bac7(1-35) è il più breve frammento del peptide Bac7, una catelicidina ricca in prolina di 60 residui, dotato della stessa attività battericida del peptide intero. In questo lavoro di tesi, abbiamo rimarcato che sbmA è l’unico gene la cui delezione conferisce ad E. coli una resistenza a Bac7(1- 35). Tale gene codifica per una proteina della membrana interna coinvolta nell’ingresso del peptide nel citoplasma della cellula batterica. Inoltre, abbiamo dimostrato che SbmA è coinvolta anche nel trasporto transmembrana di un frammento di un altro peptide antimicrobico, l’arasina1(1-23). Tali risultati suggeriscono che questa proteina giochi un ruolo generale nell’internalizzazione di peptidi antimicrobici ricchi in prolina nei batteri Gram-negativi. Abbiamo quindi misurato la concentrazione intrabatterica raggiunta da Bac7(1-35) in E. coli e abbiamo osservato che questa aumenta da valori micromolari nel terreno di coltura a millimolari nel citosol batterico, suggerendo un suo legame a strutture interne della cellula. Per questo abbiamo cercato possibili interazioni tra il peptide e macromolecole coinvolte in processi vitali del batterio. Con questi studi abbiamo appurato che Bac7(1-35) in vitro inibisce completamente il processo di trascrizione/traduzione a partire da una concentrazione di 50 μM. Successivamente abbiamo dimostrato che questa inibizione è una peculiarità di Bac7(1-35), in quanto altri AMP derivati da catelicidine ma non ricchi in prolina non hanno dimostrato un’attività comparabile. Inoltre, questa inibizione non è stereo-specifica, in quanto anche l’isomero D di Bac7(1-35) blocca tale processo esattamente come il suo isomero L. Abbiamo inoltre dimostrato la capacità di Bac7(1-35) di legare in vitro il DNA, ma abbiamo escluso che tale legame rappresenti il meccanismo primario della sua attività battericida. Abbiamo anche dimostrato che il peptide non interferisce in vitro in maniera significativa con il processo di trascrizione, deducendo che l’effetto osservato sul processo di trascrizione/traduzione fosse da attribuirsi prevalentemente all’inibizione della traduzione. Abbiamo verificato tali dati in vivo su cellule di E. coli misurando l’incorporazione di precursori radioattivi delle macromolecole batteriche. Abbiamo osservato che l’esposizione di batteri a Bac7(1-35) bloccava l’incorporazione di leucina radioattiva ma non di timidina ed uridina, indicando un blocco specifico della sintesi proteica ma non di quelle di DNA e RNA. In futuro, una definizione ancora più chiara del target intrabatterico di Bac7(1-35) potrebbe portare alla sperimentazione di tale molecola o di suoi analoghi come farmaci antibiotici di nuova generazione.
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Wiza, Claudia [Verfasser], and Michael [Akademischer Betreuer] Feldbrügge. "Proline-rich Akt substrate of 40 kDa (PRAS40): A new modulator and target of insulin action. Characterization of the function of PRAS40 in Akt- and mammalian target of rapamycin complex 1 (mTORC1)-signaling pathway. / Claudia Wiza. Gutachter: Michael Feldbrügge." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2014. http://d-nb.info/1046670646/34.
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