Добірка наукової літератури з теми "Probe pulse generator"

AbstractAtom probe has been developed for investigating materials at the atomic scale and in three dimensions by using either high-voltage (HV) pulses or laser pulses to trigger the field evaporation of surface atoms. In this paper, we propose an atom probe setup with pulsed evaporation achieved by simultaneous application of both methods. This provides a simple way to improve mass resolution without degrading the intrinsic spatial resolution of the instrument. The basic principle of this setup is the combination of both modes, but with a precise control of the delay (at a femtosecond timescale) between voltage and laser pulses. A home-made voltage pulse generator and an air-to-vacuum transmission system are discussed. The shape of the HV pulse presented at the sample apex is experimentally measured. Optimizing the delay between the voltage and the laser pulse improves the mass spectrum quality.

Electrostatic discharge is a familiar interfere. Repeatability of discharge current is poor. Transmission line pulse (TLP) is used in study of immunity of a microcontroller. A TLP generator is first manufactured with the rise time of output pulse less than 1ns. Then couplings by electric field probe, magnetic field probe and direct injection are presented. And immunity test of a microcontroller is done by using the TLP generator and probes. Results show that the tested microcontroller is more sensitive to magnetic field interference; reset pin and crystal oscillator pin of the chip are more sensitive than other pins under action of direct injection interference.

1. A low-spinal, immobilized turtle displays a fictive scratch reflex in hindlimb motor neurons in response to tactile stimulation of the shell (17, 19). Turtles exhibit three forms of the scratch reflex: rostral, pocket, and caudal. Each form is elicited by tactile stimulation of a different receptive field on the body surface. The ventral-posterior pocket (VPP) cutaneous nerve innervates the ventral-posterior portion of the pocket scratch receptive field (Fig. 1). Natural stimulation within the VPP nerve's receptive field evoked a pocket scratch reflex (Fig. 2A). Electrical stimulation of this nerve elicited robust pocket scratch reflexes (Fig. 2, B and C). 2. A single electrical pulse to the VPP nerve delivered at a voltage (greater than 5 V, 0.1 ms) that activated all the axons in the nerve was termed a "maximal" pulse. A single maximal pulse did not evoke a scratch motor response. It raised the excitability of the pocket scratch central pattern generator for several seconds, however. We revealed such excitability changes by applying maximal pulses to the VPP nerve at multisecond intervals (Figs. 5 and 6). When we delivered maximal pulses with interpulse intervals of less than or equal to 5 s, the first pulse produced no motor response and the second pulse evoked one or more cycles of pocket scratch. 3. A stimulus pulse applied to the VPP nerve was used as a probe for studying changes in the excitability of the pocket scratch CPG following scratch motor patterns. In a rested preparation, the stimulus pulse did not activate motor output. In contrast, the stimulus pulse evoked one or two cycles of pocket scratch activity if delivered within 2.5 s after the cessation of rhythmic pocket scratch motor activity (Figs. 7-9). These results are consistent with the hypothesis that the pocket scratch CPG has elevated excitability for seconds following the cessation of pocket scratch motor output. A single pulse applied to the VPP nerve evoked no response if delivered after the cessation of rostral scratch motor activity, however (Fig. 9D). 4. We used a train of maximal pulses to the VPP nerve to probe the form-specificity of the changes in the excitability following a rostral scratch motor pattern (Fig. 10). We set the stimulus parameters so that the train evoked one or two cycles of a pocket scratch motor pattern in a preparation that had rested for over 1 min.(ABSTRACT TRUNCATED AT 400 WORDS)

This study demonstrates nano-scale lithography process on localized (100) silicon (p-type) surface using modified AFM apparatuses and controlling methods. AFM-based experimental apparatuses are connected the customized pulse generator that supplies electricity between conductive tip and silicon surface maintaining constant humidity during processes. Then pulse durations are controlled according to various experimental conditions. The pulsed electrochemical reaction within the gap between conductive tip and silicon surface induces the formation of oxide with nano-scale topographies. Various heights and widths of oxides can be created by AFM surface modification according to various pulse durations and applied electrical conditions under humidity environment. In addition, it can be known that oxides are completely removed after sample surface is etched in diluted HF solution, which shows micro/nano-scale grooves can be fabricated after predefined chemical treatment. They are wider than oxides widths and have several nanometer depths. Nano patterning technique from this experiment suggests that pulse electrochemical machining process has bright potential for advancing nano machining technologies.

Abstract Context Kisspeptin–neurokinin B (NKB)–dynorphin neurons are critical regulators of the hypothalamic–pituitary–gonadal axis. NKB and dynorphin are hypothesized to influence the frequency of GnRH pulses, whereas kisspeptin is hypothesized to be a generator of the GnRH pulse. How these neuropeptides interact remains unclear. Objective To probe the role of NKB in GnRH pulse generation and to determine the interactions between NKB, kisspeptin, and dynorphin in humans and mice with a complete absence of NKB. Design Case/control. Setting Academic medical center. Participants Members of a consanguineous family bearing biallelic loss-of-function mutations in the gene encoding NKB and NKB-deficient mice. Interventions Frequent blood sampling to characterize neuroendocrine profile and administration of kisspeptin, GnRH, and naloxone, a nonspecific opioid receptor antagonist used to block dynorphin. Main Outcome Measures LH pulse characteristics. Results Humans lacking NKB demonstrate slow LH pulse frequency, which can be increased by opioid antagonism. Mice lacking NKB also demonstrate impaired LH secretion, which can be augmented with an identical pharmacologic manipulation. Both mice and humans with NKB deficiency respond to exogenous kisspeptin. Conclusion The preservation of LH pulses in the absence of NKB and dynorphin signaling suggests that both peptides are dispensable for GnRH pulse generation and kisspeptin responsiveness. However, NKB and dynorphin appear to have opposing roles in the modulation of GnRH pulse frequency.

A compact plasma system is set up at Ravenshaw University, India. The plasma system consists of a curved vacuum chamber which is nothing but a part of a toroid (θ=700) having minor radius, r= 0.3 m and major radius, R= 0.5 m, vacuum system, electromagnet, gas injected washer stacked plasma gun to produce plasma blobs/filaments, pulse forming network to energise plasma gun, diagnostic tools like electric probes, magnetic probes, spectrometer, high speed CCD camera, digital pulse/delay generator to synchronise the diagnostic tools. A pair of copper coil is wound over the chamber and capacitive pulse is fed to the coil to produce non-uniform magnetic field inside the chamber. The gas injected washer stacked plasma gun is a mono-anode - multi cathode system having five cathodes made up of brass and an anode made up of copper. The gun impedance is ~ 15 Ω. The pulse forming network (PFN) is Guillemin E type which consists of capacitors having equal capacitance 5.5 μF and inductors having equal inductances 1.5 μH. The pulse width of the PFN is ~ 7.6 μs for a seven stage network, as tested with known resistive circuit. Magnetic probes are designed and calibrated using a Helmholtz coil to map the radial magnetic field profile of the plasma chamber. Electric probes like Langmuir triple probe, velocity probes are designed to measure plasma parameters like blob velocity, density, temperature etc. Emission spectroscopy method is used to identify charged species inside the plasma. High speed CCD camera is used to interpret the structure of the plasma. A digital pulse/trigger generator is used to synchronise the CCD, spectrometer and switching thyristor etc. Preliminary results are also reported.

The conventional eddy current testing uses a sinusoidal signal with very narrow frequency bandwidth. Whereas, the pulsed eddy current method uses a pulse signal with a broad frequency bandwidth. This allows multi-frequency eddy current testing, and the penetration depth is greater than that of the conventional eddy current testing. In this work, the pulsed eddy current instrument was developed for evaluating the metal loss. The developed instrument was consist of the pulse generator generating the square pulse of maximum 40 V, the amplifier controlled to 52dB, the A/D converter of 16bit 20MHz, and the industrial personal computer for operating with Windows program. And, the probe for the pulsed eddy current was designed as the pancake type in which the sensing coil was located in the driving coil. The peak voltage did not linearly increase with the voltage of the step pulse. For the driving coil with inductance of 670µH, the peak voltage linearly increased with the step pulse voltage to 30V. But, for the other driving coils with the inductance of 1.7mH, 2.7mH, 3.6mH, 22mH, the peak voltage linearly increased with the step pulse voltage to 20V. The output signals of the sensing coil rapidly increased when the step pulse driving voltage was off, and the latter part of the sensing coil output voltage exponentially decreased with a time. The decrement value of the output signals of sensing coil increased with the thickness of the aluminum test piece.

It is possible to increase the sensitivity of electromagnetic-acoustic transducers by three main methods: increasing the induction of a polarizing magnetic field; increase in strength of high-frequency current in the inductor of the converter; using modern methods of processing information packet pulses excited and received from the product. The increase in magnetic field induction of the converter is limited by the capabilities of modern powerful permanent magnets. In addition, there are significant difficulties in monitoring ferromagnetic control samples, due to the large pressing force between the sample and the transducer and sticking of scale to the transducer. Usage of modern processing methods significantly complicates and increases the cost of electromagnetic-acoustic testing devices. Of the selected sensitivityincreasingmethods, the most acceptable way is to increase the power of high-frequency current generators under the condition of excitation of packet pulses. Power supply process of the converter is carried out in two stages. Meander type powerful high-frequency current pulses are excited, which ensures operation of the output transistors in key mode. Isolation of a powerful sinusoidal high-frequency packet current pulse is carried out directly on the electromagnetic - acoustic transducer, the elements of which are included in the resonant circuit with low quality factor. Based on the transistors switched in the push-pull circuit in the key mode, a small-sized generator design has been created that excites a peak current of up to 800 A in the electromagnetic-acoustic transducer and a voltage of up to 3 kV in the transducer in the frequency range 0.3 ... 8 MHz. It was experimentally determined that the new design of the probe pulse generator made it possible to increase the amplitude of pulses reflected from a flat-bottom reflector with a diameter of 2 mm with respect to the interference amplitude by more than 2 times.

With good repeatability and simple structure, transmission line pulse (TLP) has been used in immunity test of integrated circuit and printed circuit board. A TLP generator is first manufactured and its output waveform is presented. By using wavelet transform, the waveform is denoised and discriminated to components inherent to system function and parasitic parameters. Frequency spectrum changed with time is also obtained by continuous wavelet transform of complex morlet. Decomposed damping oscillation component and high frequency component in instant frequency spectrum show influence of inductance in circuit on the waveform. Improvement of rising time and overshoot is achieved by change of probe connection with shorter grounding line.

Introduction. At present the most accurate estimate of ranges is specific to laser range finders using phase measuring techniques. Design of a pulsed laser range finder with short probe pulses enabling one to gain high resolution and accuracy of estimate of target range close to the phase range finders is the topical problem.Aim. Development of a receiving part of the pulsed laser rangefinder with precision characteristics; determination of the accuracy of the measurements; description of the hardware.Materials and methods. The construction of the receiving part of the precision pulsed laser rangefinder with a two-scale digital range estimation system implemented by counting clock generator pulses and an analog integrator that specifies the discrete range estimation was considered. Using the methods of mathematical statistics, the energy characteristics of the rangefinder were determined: the accuracy of the range estimation and the probability of false alarm were provided by the developed scheme. The hardware of the precision laser rangefinder was described.Results. The principles of implementation of the receiving part of the laser rangefinder with a two-scale digital system for estimating the distance to the object were given. The results of numerical simulation of rangefinder characteristics were obtained, confirming the accuracy of range estimation of the order of millimeters. In the implemented rangefinder scheme, the probability of false alarm was 10-4 during 200 s of observing signal and noise mixture. The hardware of the precision laser rangefinder with a digital two-scale range estimation was proposed.Conclusion. The implemented laser range finder approaches to the capabilities of phase laser rangefinders in terms of potential accuracy of distance up to millimeters, while implementing the specified parameter in rapidly changing phono-target environment. Using of short probing pulses with a duration of 10...20 ns allows one to achieve a resolution of up to 1.5 m. In contrast to the phase rangefinder the range can be estimated from a single probe pulse.

Дисертація на здобуття наукового ступеня кандидата технічних наук (доктора філософії) за спеціальністю 05.11.13 «Прилади і методи контролю та визначення складу речовин» – Національний технічний університет «Харківський політехнічний інститут». Дисертація присвячена розробці нових ультразвукових електромагнітно-акустичних перетворювачів з джерелом імпульсного поляризуючого магнітного поля, методів підвищення чутливості контролю та діагностики металовиробів з використанням перетворювачів такого типу. Виконано аналітичний огляд та аналіз сучасних засобів і методів контролю та діагностики електромагнітно-акустичним методом [1–3] феромагнітних і електропровідних або тільки електропровідних виробів в умовах дії постійних та імпульсних поляризуючих магнітних полів з урахуванням наявності когерентних завад різного типу, технічного рівня сучасних електромагнітно – акустичних перетворювачів, схемотехнічних рішень засобів їх живлення, прийому з виробів ультразвукових імпульсів та їх обробки, визначення відомих переваг, недоліків та можливостей використання в дослідженнях і розробках. Визначені та обґрунтовані напрямки дисертаційного дослідження: розробка електромагнітно-акустичного перетворювача у вигляді спрощеної одновиткової моделі [4] джерела магнітного поляризуючого поля з феромагнітним осердям та високочастотною котушкою, яка розміщена між осердям та металовиробом; шляхом моделювання [5] розподілення індукції поляризуючого магнітного поля на торці осердя джерела магнітного поля та в поверхневому шарі як феромагнітного так і неферомагнітного металовиробу визначено особливості розташування високочастотної котушки індуктивності під джерелом магнітного поля для ефективного збудження зсувних ультразвукових імпульсів (в центральній частині торця феромагнітного осердя) або поздовжніх ультразвукових імпульсів (біля периферійної частини торця феромагнітного осердя) [6]. Збільшення кількості витків котушки намагнічування при наявності феромагнітного осердя призводить до значного збільшення часу перехідних процесів при включенні живлення імпульсного джерела поляризуючого магнітного поля і при його виключенні. В результаті час дії імпульсу живлення збільшується до 1 мс і більше, що призводить до збільшення сили притягування ЕМАП до феромагнітного виробу, додаткових втрат електроенергії, погіршенню температурного режиму перетворювача. Для зменшення часу дії імпульсу живлення джерела магнітного поля необхідно зменшувати кількість витків котушки намагнічування, але це призводить до зменшення величини магнітної індукції навіть при наявності феромагнітного осердя. В результаті раціонального вибору конструкції джерела магнітного поля встановлена необхідність виконання його котушки намагнічування плоскою двовіконною трьохвитковою і виготовляти з високоелектропровідного високотеплопровідного матеріалу [7-9]. Осердя повинно бути розміщено в вікнах котушки намагнічування тільки торцями. В результаті час дії імпульсу намагнічування зменшено до 200 мкс, що достатньо для контролю виробів товщиною до 300 мм. Високочастотна котушка індуктивності виконана з двома лінійними робочими ділянками, які розташовуються під вікнами котушки намагнічування [9]. При протилежних напрямках високочастотного струму в цих робочих ділянках в поверхневому шарі виробу збуджуються синфазні потужні імпульси зсувних ультразвукових хвиль. При цьому відношення збуджуваних амплітуд зсувних та поздовжніх імпульсів перевищує 30 дБ. Тобто когерентні імпульси поздовжніх хвиль при контролі луна методом практично не будуть впливати на результати діагностики феромагнітних виробів. Розроблені варіанти конструкцій електромагнітно-акустичних перетворювачів з одновитковими [7], двовитковими [8] та трьохвитковими [9] котушками намагнічування джерела імпульсного поляризуючого магнітного поля. При одновитковій котушці [7] перехідні процеси при включенні імпульсу живлення мінімальні. Проте необхідно збуджувати в котушці струм з силою в кілька кА, що ускладнює температурний режим перетворювача та апаратуру живлення. При трьохвитковій котушці [9] намагнічування амплітуда донних імпульсів по відношенню до амплітуди завад перевищує 24 дБ, що дозволяє проводити контроль та діагностику значної кількості металовиробів. При використанні шихтованого осердя [9] відношення амплітуд корисного сигналу і шуму збільшилося до 38 дБ, що дає можливість проводити ультразвуковий контроль лунаметодом. Розроблено метод [10 ] ультразвукового електромагнітно- акустичного контролю феромагнітних виробів, суть якого заключається в збудженні ультразвукових імпульсів шляхом формування в поверхневому шарі феромагнітного виробу двох рядом розташованих короткочасно намагнічених ділянок з протилежним напрямком векторів магнітної індукції поляризуючого поля, збудженні в намагнічених ділянках пакетних імпульсів електромагнітного поля з протилежно направленими векторами напруженості тривалістю в кілька періодів високої частоти заповнення, при цьому збудження імпульсів електромагнітного поля виконують в момент часу, який дорівнює часу перехідних процесів з встановлення робочої величини індукції поляризуючого магнітного поля, а прийом ультразвукових імпульсів відбитих з виробу виконується в період часу tпр, який визначається за виразом T – t1 – t2 – t3 < tпр = t1 + t2 + t3 + 2H/C, де Т – тривалість імпульсу намагнічування; t1 – час перехідних процесів з встановлення робочої величини індукції поляризуючого магнітного поля; t2 – час дії пакетного імпульсу електромагнітного поля; t3 – час затухаючих коливань в плоскій високочастотній котушці індуктивності; Н – товщина виробу або відстань в об’ємі виробу, які підлягають ультразвуковому контролю; С – швидкість поширення зсувних ультразвукових хвиль в матеріалі виробу. Встановлено [9] [9], що завади в феромагнітному осерді, обумовлені ефектом Баркгаузена та магнітострикційним перетворенням електромагнітної енергії в ультразвукову при збудженні ультразвукових імпульсів, практично виключаються за рахунок виготовлення осердя шихтованим, матеріал пластин осердя повинен мати низький коефіцієнт магнітострикційного перетворення, пластини осердя повинні бути орієнтовані перпендикулярно провідникам робочих ділянок плоскої високочастотної котушки індуктивності, а також заповненням щілин між пластинами осердя рідиною із значною густиною, наприклад гліцерином. Показано, що чутливість прямих ЕМА перетворювачів з імпульсним намагнічуванням при живленні розробленим генератором пакетних зондуючих високочастотних імпульсів [11 ] та прийомі малошумлячим підсилювачем [12 ] забезпечують виявлення плоскодонних відбивачів діаметром 3 мм і більше при частоті зондування 40 Гц, піковому високочастотному струмі 120 А, частоті зсувних лінійно поляризованих ультразвукових коливань 2,3 МГц, тривалості високочастотного пакетного імпульсу 6…7 періодів частоти заповнення, тривалості імпульсу намагнічування 200 мкс, густині струму намагнічування 600 А/мм2 та при зазорі між ЕМАП і виробом 0,2 мм [9] [9]. При цьому амплітуда луна імпульсу відбитого від дефекту по відношенню до амплітуди завад досягає 20 дБ. Розроблені ЕМАП захищені 2 патентами на корисну модель.
Thesis for a Candidate Degree in Engineering (Doctor of Philosophy), specialty 05.11.13 "Devices and methods of testing and determination of composition of substances" - National Technical University "Kharkiv Polytechnic Institute". The dissertation is devoted to development of new ultrasonic electromagnetic-acoustic transducers with a source of pulsed polarizing magnetic field, methods of sensitive testing and diagnostics of metalware with the use of transducers of this type. Analytical review and analysis of modern means and methods of testing and diagnostics via electromagnetic-acoustic method [1-3] of ferromagnetic and electrically conductive or strictly electrically conductive products under conditions of impact of constant and pulse polarizing magnetic fields taking into account the presence of coherent interferences of different types, technical level of modern electromagnetic circuits, means of their power supply, reception of ultrasonic pulses from metalware and their processing, determination of known advantages and disadvantages, and opportunities of their use in research and development. The direction of the research is defined and justified: development of electromagnetic-acoustic transducer in the form of a simplified single-wind coil model [4] of a source of a magnetic polarizing field with a ferromagnetic core and a high-frequency coil, which is located between the core and the sample; by modeling [5] the distribution of induction of polarizing magnetic field at the end face of the core of the magnetic field source and in the surface layer of both ferromagnetic and non-ferromagnetic metallurgy the features of the location of the high frequency coil of inductance under the magnetic field source are effectively determined for the effective excitation of shear ultrasonic pulses (near the peripheral end of the ferromagnetic core) [6]. The increase in number of winds of magnetization coil in presence of a ferromagnetic core leads to a significant increase in time of transients during the process of powering of a pulsed source of a polarizing magnetic field and during its switching off. As a result, the duration of the power pulse increases to 1 ms or more, which leads to an increase in the force of attraction of EMAP to the ferromagnetic product, additional losses of electricity, deterioration of temperature conditions of the transducer. To reduce the duration of powering pulse of magnetic field it is necessary to reduce the number of winds of the magnetizing coil, but this leads to a decrease in magnetic induction magnitude, even in presence of a ferromagnetic core. As a result of rational choice of the design of the magnetic field source, the flat coil of magnetization must be made with a two-window three-wind and made of high-conductive high-heat-conducting material [7-9]. The core should be placed in the windows of the magnet coil only by the ends. As a result, the action time of the magnetization pulse is reduced to 200 μs, which is sufficient for testing of samples up to 300 mm thick. The high-frequency inductor coil is made of two linear working sections that are located under the windows of the coil [9]. In opposite directions of high-frequency current in these working areas, in-phase powerful pulses of shear ultrasonic waves are excited in the surface layer of the product. The ratio of the excited amplitudes of the shear and longitudinal pulses exceeds 30 dB. That is, the coherent pulses of longitudinal waves in the testing of the moon by the method will practically not affect the results of the diagnosis of ferromagnetic products. Design variants of electromagnetic-acoustic transducers with one-wind [7], two-wind [8] and three-wind magnetization coils [9] of a source of a pulsed polarizing magnetic field are developed. With a single-coil [7], the transients are minimal when the power pulse is winded on. However, it is necessary to excite in the coil a current of several kA, which complicates the temperature conditions of the transducer and power equipment. With a three-coil [9] magnetization, the amplitude of the bottom pulses in relation to the amplitude of the interference exceeds 24 dB, which allows for testing and diagnostics of large variety of samples. When using the charge core [9], the ratio of amplitudes increased to 38 dB, which makes it possible to monitor the echo by the method. The method [10] of ultrasonic electromagnetic - acoustic testing of ferromagnetic products is developed. vectors of intensity with duration of several periods of high filling frequency, n and this excitation of the pulses of the electromagnetic field is performed at a time equal to the time of transients to establish the operating value of the induction of the polarizing magnetic field, and the reception of ultrasonic pulses reflected from the product is performed in the time period tпр, which is determined by the expression T – t1 – t2 – t3 < tпр = t1 + t2 + t3 + 2H/C, where T is the duration of the magnetization pulse; t1 is the time of transients to establish the working value of the induction of a polarizing magnetic field; t2 - time of packet pulse of electromagnetic field; t3 is the time of damping oscillations in the flat high frequency inductor; H is the thickness of the product or the distance in volume of the product to be ultrasound; C is the velocity of propagation of shear ultrasonic waves in the material of the product. It is established [9] that the interferences in the ferromagnetic core caused by the Barkhausen effect and magnetostrictive transformation of electromagnetic energy into ultrasound are practically excluded by production of the core blended, usage of the material of the core plates which has a low coefficient of magnetostrictive conversion, perpendicular core plates orientation in relation to the conductors of the working areas of the flat high-frequency inductor, as well as filling of the gaps between the plates with a high density fluid, such as glycerol. It is shown that the sensitivity of direct EMA transducers with pulse magnetization when powered by a batch high frequency probe pulse generator [11] and when receiving via a low noise amplifier [12] provide detection of flat-bottomed reflectors with a diameter of 3 mm or more, probe frequency of 40 Hz, peak high-frequency current of 120A, shear linearly polarized ultrasonic oscillations of 2.3 MHz, high frequency packet pulse duration 6…7 filling frequency periods, magnetization pulse duration 200 μs, magnetization current density of 600 A / mm2 and at the gap between the EMAP and the product of 0.2 mm [9]. The amplitude of the echo momentum reflected from the flaw in relation to the noise amplitude reaches 20 dB. The EMATs developed are protected with 2 utility model patents.

Дисертація на здобуття вченого ступеня кандидата технічних наук за спеціальністю 05.11.13 – прилади і методи контролю та визначення складу речовин. Національний технічний університет «Харківський політехнічний інститут», Харків, 2020. В дисертаційній роботі вирішено актуальну науково-практичну задачу з розробки нових типів ЕМАП для ефективного ультразвукового контролю металовиробів. В роботі виконано комп’ютерне моделювання розподілу магнітних полів ЕМАП при імпульсному намагнічуванні феромагнітних та немагнітних виробів. Встановлені шляхи побудови перетворювачів з максимальною чутливістю. Розроблено метод збудження імпульсних пакетних ультразвукових імпульсів за рахунок послідовного в часі формування імпульсного магнітного та електромагнітного полів. Розроблено технічні рішення пригнічення когерентних завад в осерді та у виробі. Визначені геометричні та конструктивні параметри джерела імпульсного магнітного поля, що дало можливість збуджувати потужні синфазні пакетні імпульси високочастотних зсувних коливань в ОК. Показано, що чутливість прямих ЕМА перетворювачів з імпульсним намагнічуванням забезпечують виявлення плоскодонних відбивачів діаметром 3 мм і більше при частоті зондування 40 Гц, частоті зсувних лінійно поляризованих ультразвукових коливань 2,3 МГц, піковому струмі високочастотних пакетних імпульсів 120 А, тривалості пакетних високочастотних імпульсів струму в 6 періодів частоти заповнення, тривалості імпульсу намагнічування 200 мкс, щільності струму намагнічування 600 А/мм2 та при зазорі між ЕМАП і виробом 0,2 мм. При цьому амплітуда луна-імпульсу від дефекту по відношенню до амплітуди завад досягає 20 дБ, що дає можливість забезпечити якісну дефектоскопію металовиробів.
Thesis for a Candidate Degree in Engineering, specialty 05.11.13 – Devices and methods of testing and determination of composition of substances. National Technical University “Kharkiv Polytechnic Institute”, Kharkiv, 2020. A relevant scientific – practical problem on development of new types of EMAP for effective ultrasonic control of metal products is solved in the dissertation. Computer simulation of EMAT magnetic fields distribution in pulse magnetization of ferromagnetic and non-magnetic products is performed. Ways to build transducers with maximum sensitivity are established. The method of excitation of pulsed batch ultrasonic pulses due to the sequential formation of pulsed magnetic and electromagnetic fields is developed. Technical solutions for suppression of coherent interference in the core and in the product have been developed. The geometrical and structural parameters of pulsed magnetic field source were determined, which made it possible to excite powerful in-phase packet pulses of high-frequency shear oscillations in a sample. It is shown that the sensitivity of direct EMA transducers with pulse magnetization provide detection of flat-bottom reflectors with a diameter of 3 mm and more at a probing frequency of 40 Hz, a frequency of shear linearly polarized ultrasonic oscillations of 2.3 MHz, a peak current of high-frequency packet pulses of 120 A, duration of batch high frequency current pulses in 6 periods of filling frequency, magnetization pulse duration of 200 μs, magnetization current of 600 A and at the gap between EMAP and product of 0.2 mm.

This thesis describes a research investigation into novel probes for the measurement of ultra-fast voltage pulses with a peak value up to 500 kV, and during the course of the work five probes were constructed for voltages of between 60 kV and 500 kV. Essential requirements of the probes are that they should be immune to high-levels of electromagnetic noise and also isolate the measuring equipment from the high voltage of the test circuit. Their designs were therefore based on an electro-optic (Pockels) Cell rather than on an electromagnetic device. The first item in the probe is a capacitive divider that attenuates the high-voltage under investigation to the level that can be fed to a Pockels Cell. Light from a laser is circularly polarized and passes through the Cell, with the attenuated voltage pulse causing the Cell crystal to change its molecular shape. This produces a change in the refractive index of the crystal and the emerging light signal becomes elliptically polarized. After conversion into electrical form, a waveform is displayed on an oscilloscope that is an accurate representation of the input voltage to the capacitor. To test the performance of a probe requires a generator capable of producing the required high voltage with a very short rise time, and a number of these were developed for use with the different probes. Careful comparisons of the performance with that of several commercially available probes showed unequivocally that the new probes were far superior. The thesis concludes by presenting ideas for future probe designs and suggests what form the ultimate probe might take. Much of the work reported in the thesis has already been presented at major international conferences or in prestigious academic journals.

An ultrafast UV-visible spectrometer was designed and implemented. An optical parametric amplifier was constructed to be used as a pump source for the spectrometer. Using nonlinear optical processes and an 800 nm ultrashort pulses, tunable infrared(IR) light was produced with a wavelength range of ∼.1 mum to 3 mum. The IR light was then mixed with 800 nm light to produce tunable visible light with a wavelength range of 466 nm to 600 nm. Supercontinuum (SC) was used as the probe pulse of the spectrometer, providing a large observation bandwidth. Commercially purchased fast spectrometers were used as the detection mechanism. The characterization of the set up, as well the observation of some ultrafast molecular dynamics observed in 8-hydroxy-1,3,6-pyrenetrisulfonic acid, are presented.

This thesis presents HELIOS, an in-house laboratory for time-resolved pump-probe spectroscopy with extreme-ultraviolet (XUV) probe radiation. A wide span of pump wavelengths can be generated using commercial laser equipment while XUV probe radiation is generated via a high harmonic generation process in a noble gas delivering probe photons with energies between 20 eV and 72 eV. The XUV beam path features a time-preserving monochromator and was constructed and built in-house. HELIOS features an overall time resolution of about 50 fs when using 800 nm pump and 41 eV probe photons. An energy resolution of 110 meV at 41 eV photon energy can be achieved. HELIOS features two beamlines. One µ-focus beamline with an XUV focal size of about 20 µm can be used with experiments that require such a small XUV focal size as well as with different end stations. The other beamline features a semi-permanently mounted end station for angle-resolved photoelectron spectroscopy under ultra-high vacuum conditions. Experiments demonstrating the usability of HELIOS and the two beamlines are presented. A pump-probe measurement on graphene demonstrates the capability of determining a large part of the k-space in only one measurement due to the use of an ARTOF angle-resolved time-of-flight electron spectrometer. A non-angle-resolved pump-probe measurement on the conducting polymer PCPDTBT demonstrates the high signal-to-noise ratio achievable at this beamline in non-angle-resolved photoelectron-spectroscopy pump-probe measurements. The usability of the µ-focus beamline is demonstrated with time-resolved measurements on magnetic samples employing an in-house-designed spectrometer. These experiments allow the retrieval of element-specific information on the magnetization within a sample employing the transversal magneto-optical Kerr effect (T-MOKE). Additionally, a Fourier transform spectrometer for the XUV is presented, the concept was tested at a synchrotron and it was used to determine the longitudinal coherence of the XUV radiation at HELIOS.

碩士
國立中央大學
物理學系
105
Graphene has attracted attention in recent years because of low dimensional and high electron mobility. However, the gap-less feature is the main obstacle to further electronic application. Defect generation is one way to manipulate the band gap of graphene. To create defect on graphene, Scanning probe lithography (SPL) is a well-developed nano-meter scale technique. In our previous work, we formed graphene oxidation through negative bias SPL. However, the mechanism of the oxidation processing with SPL is still unveiled. To understand this, we set up a pulsed SPL system with precise pulse width, pulse treatment position control and the output impedance control. After point-like arrays are generated by pulsed SPL, both Raman and atomic force microscopy (AFM) measurements conclude that those defects are holes in average diameter 160 nm on graphene. In the limit of maximum current, ring-like patterns are generated. It indicates that the point-like holes are created by large charging current and that the ring patterns are caused by electrolysis which is driven by voltage. In summary, the point-like and ring-like patterns represent current dominant and voltage dominant phase in the charging process.

碩士
國立中正大學
物理所
97
Eumelanin is one of the most ubiquitous biological pigments found in human body. Despite many decades of studies, people still do not have a complete picture of its macromolecular structure. Since eumelanin has a well-known, broad-band monotonic absorption, studying the dynamics of light absorption becomes an important method to investigate the structure of eumelanin. From the degenerate UV pump-probe experiment by Simon et al., the optical absorption spectrum of the monomer and tetramer composing of four monomers found to be quite different is quite different. We use the whitelight-generation pulse as probe beam to perform the femtosecond pump-probe experiment to explore the structure dynamics of eumelanin. In chapter 1, we introduce the structure of eumelanin and review the related experiment in the past. In chapter 2, we describe the laser system, the experimental setup and parameters used in the experiment. In charpter 3, we show the experiment results and propose an appropriate model to explain the data. The last chapter is the conclusion of this study and possible follow-up works.

The electronic readout and processing of detector signals, generated by radiation in detectors, is today by far the most common form of signal acquisition in particle physics. In this chapter typical procedures for electronic readout of detectors are discussed with special attention to small, noise-prone signals. An overview is given of standard techniques for signal processing, like amplification, pulse shaping, discrimination and digitization where also the new developments in microelectronics are discussed. In applications with high data rates, as at modern accelerator experiments or also in (X-ray) image processing, deadtimes can occur which are discussed in a dedicated section. Similarly, there is a section on wave guide properties of signal cable. Often the signals are so small, in particular those of semiconductor detectors, that electronic noise and its suppression play an important role.

Most of the NMR spectra shown in this book and in the literature have been recorded at 250 or 300 MHz, with a few being obtained at 500 MHz for 1H NMR (the equivalent for 13C NMR). No special pulse sequences are necessary, just standard one-dimensional (1D) spectra although two-dimensional (2D) experiments (e.g., correlation spectroscopy; COSY) may be necessary in some cases in order to get an unambiguous identification of the signals relevant for the assignment. In general, 5–10 mg or less of CDA derivative dissolved in 0.5 mL of deuterated solvent are sufficient to obtain a good NMR spectrum. Temperature, solvent, and concentration used in the NMR experiments should be adequate for each CDA-substrate pair and methodology, because the method is based on the conformational composition of the AMAA derivatives in precise conditions. With the exception of the low-temperature procedure (single derivatization), a NMR probe temperature around 300 K has always been used. In general, the spectra for double-derivatization assignments should be taken in deuterated chloroform. Different NMR solvents are required only in two of the single-derivatization methods. In the assignment by low-temperature NMR, the most convenient solvent is a CS2/CD2Cl2 (4:1) mixture, which allows the use of temperatures low enough (i.e., 213 K) to obtain relevant shifts. In the procedure based on the complexation with Ba2+, the NMR solvent should be deuterated acetonitrile. The barium salt is anhydrous Ba(ClO4)2, which can be added directly to the tube by using a spatula. No weighing is necessary after shaking, as the excess salt will remain at the bottom of the NMR tube and will not disturb the experiment. (R)- and (S)-MPA, MTPA, and Boc-phenylglycine (BPG) are commercially available and can be used without further purification. The first two (MPA and MTPA) can also be purchased as acid chlorides. When using MTPA or the corresponding acid chloride [85] for the derivatization of an alcohol or amine, it should be noted that the Cahn-Ingold-Prelog priority rules assign different R/S descriptors to the acid and to the corresponding chloride; this is due to the different priority order generated by the substituents [i.e., (R)-MTPA generates the (S)-acid chloride and vice versa].

High-sensitivity, single-molecule detection in flow is a paradigm that has been defined at Los Alamos over the last two decades. A recent focus has been on applications of single- molecule detection for DNA fragment sizing using a compact, low-power, highsensitivity flow cytometer (HSFCM). There are three key aspects of our approach that distinguish it from conventional flow cytometry and yield the high level of sensitivity that we achieve: a detector with high photon-detection efficiency, a small probe volume to reduce background noise, and slow flow to provide extended analyte dwell time in the probe volume. An additional factor for applications in DNA fragment sizing is a DNA stain with significant fluorescence enhancement when bound to double-stranded DNA, and low background fluorescence in the unbound state. DNA fragment sizing by HSFCM has important applications in bacterial species and strain identification, where it can replace the cumbersome and time-consuming pulsed-field gel electrophoresis (PFGE) approach routinely used by public health labs for bacterial identification. The revolutionary capability to interrogate single DNA molecules, as well as potentially other submicron-sized biological particles, in a high-sensitivity flow cytometer will provide new scientific insights into cellular and molecular biology and introduce high-sensitivity flow cytometry to a wide variety of new applications in biotechnology. Flow cytometry has enabled major advances in the biomedical sciences by providing rapid, quantitative, and sensitive multiparameter measurements of individual cells and subcellular particles such as chromosomes. This analysis of individual entities produces information on population heterogeneity that is not revealed in ensemble measurements and that allows more precise quantitation of distinct attributes than is possi ble when measurements are done in bulk. However, one limitation of conventional flow cytometry is the inability to measure submicron-sized particles or weakly fluorescent particles labeled with fewer than several hundred fluorophores, primarily as a result of insufficient detection sensitivity. A wide variety of important biological particles, molecules, and molecular assemblies fall into these categories. There have been many reports of bacterial measurement and characterization by conventional flow cytometry, dating back to 1947. In 1979, Steen developed a microscope-based system specifically for applications in microbiology. Many bacteria are large enough to generate a light-scatter signal, which is useful for their detection.

Different nano-patterns have been generated with the same near field scanning optical microscope (NSOM) tips with multiple femtosecond laser pulses in different background gases. It is demonstrated that significant energy was transferred from the NSOM probe to a pure silicon surface for the generation of nano-protrusions and nano-craters, which shows the possibility of nano-fabrication with the present experimental configuration. In order to understand the heating effect of the target and the relationship between the generations of nano-craters, a corresponding theoretical analysis considering the wave format light propagation within a single tapering NSOM probe (first order approximation) and the subsequent light absorption in a target is established. This analysis show that electron temperature of around the nano-scale laser spot of target can be very high (&gt;∼10,000 K) during the laser pulse. However, both the photoexcited electron number density and lattice temperature are much less the threshold for a thermal and non-thermal evaporation. Therefore, supplementary mechanisms in additional to pure pulsed light absorption are required for generation of nano-craters on a target if a single tapering angle NSOM probe is applied.

Advancements in technologies related to thin film growth have led to astoundingly complex integrated photonic devices. The reliability of these devices relies upon the precise control of the band gap and absorption mechanisms in the thin film structures. Photon absorption in these devices can result in a reduction of laser efficiency as well as thermal runaway. To improve device performance prediction, an increased understanding of the localized absorption processes is paramount. A pump-probe technique is being developed to measure the transient absorption during hot carrier relaxation. This method relies upon the generation of hot carriers by the absorption of an intense ultrashort laser pulse. The change in reflectance due to hot carrier generation and relaxation is monitored using a probe pulse focused at the center of the excited region. The transient reflectance is measured as a function of the relative delay between the pump and probe pulses. Utilizing ultrashort laser pulses (τp ∼ 190 fs) it is possible to attain sub-picosecond resolution of the transient reflectance during hot carrier relaxation. Transient changes in the reflectance can then be related to transient changes in the absorption mechanisms of the film. Preliminary measurements made with this technique have shown clear differences in the transient reflectance of doped and undoped Indium Phosphide (InP) based films. This study will form the basis for development of a transient thermoreflectance model during hot carrier relaxation in III-V semiconductors.

Acoustic Pulse Reflectometry (APR) has recently been gaining acceptance for a variety of tube inspection applications, as a viable alternative to more entrenched technologies such as eddy current. In this paper we present a case study demonstrating how APR can be used successfully for inspecting U-tube bundles. This type of heat exchanger poses a great challenge to technologies which require traversal with a probe, due to the presence of tight bends in the tubes. These are usually not traversable by probes. APR, on the other hand, uses an acoustic pulse as a “virtual probe”, with the ability to navigate bends, elbows, fittings etc. with no difficulty. In this paper we show how the various typical faults are revealed in the acoustic measurements and demonstrate how the analysis software recognizes these faults and generates the report. In one case presented here we inspected 62 heat exchangers used to heat natural gas, containing 39 U-tubes each, totaling 2379 tubes. Each tube had an internal diameter of 11mm, wall thickness of 2.5mm, and a length of approximately 6 meters, though there was some variability in length due to different lengths of the U bends. An added difficulty in inspecting these tubes was that the tube sheet was about 80 centimeters in distance from the inspection port-hole. The average inspection time in the field was 25 seconds per tube. All measurements were logged to computer files, and automated fault detection software generated a full report showing the condition of the tubes, indicating degradations in wall thickness, full and partial blockages, and holes. In the second case study we examine the variability in u-tubes in a single bundle and discuss the effect this has on the results.

In this paper, we systematically study the generation and propagation of coherent acoustic pulses in a metal-dielectric system using a two-color femtosecond pump-probe technique at different probe angles. A long-lived acoustic oscillation is observed in a borosilicate glass coated with gold and shows different attenuation and amplitude at different probe wavelength. Our study suggests that the two-color optical pump-probe technique can be utilized as a noninvasive tool to study acoustic properties of dielectric materials.

The reliability of micro-electronic devices depends on the thermal properties and energy transfer of the thin metallic films used in them. Within the thin film structures thermal transport properties often differ from bulk material and can be highly dependent on the manufacturing techniques. Thermal transport properties can be measured by the Transient ThermoReflectance technique (TTR). The TTR method uses an ultra-short laser pulse to generate a transient thermal response, and a weaker probe pulse to monitor the reflectivity response of the surface. The changes in reflectivity can be related to transient thermal effects in the film. With the use of an ultra short-pulsed laser, the extremely small time scale required to observe the microscale heat transfer phenomena is possible. The goal of this research is to observe and quantify the effect of differing substrates, film thicknesses, and deposition methods on thermal conductivity. This study uses the TTR technique to examine nickel films of varying thicknesses, fabricated on silicon, germanium, and gallium arsenide substrates, using electron beam evaporation and sputtering techniques. The thermal conductivities of the films were measured and compared.

Immersed bodies such as struts, vanes, and instrumentation probes in gas turbine flow systems will, except at the lowest of flow velocities, shed separated wakes. These wakes can have both upstream and downstream effects on the surrounding flow. In most applications, surrounding components are designed to be in the presence of a quasi-steady or at least non-variant flow field. The presence of unsteady wakes has both aerodynamic and structural consequences. Active flow control of wake generation can therefore be very valuable. One means to implement active flow control is by the use of plasma actuation. Plasma actuation is the use of strong electric fields to generate ionized gas that can be actuated and controlled using the electric fields. The controlling device can be based on AC, DC, or pulsed-DC actuation. The present research was conducted using pulsed-DC from a capacitive discharge power supply. The study demonstrates the applicability of, specifically, pulsed-DC plasma flow control of the flow on a circular cylinder at high Reynolds numbers. The circular cylinder was selected because its flow characteristics are related to gas turbine flowpath phenomena, and are well characterized. Further, the associated pressure gradients are some of the most severe encountered in fluid applications. The development of effective plasma actuators at high Reynolds numbers under the influence of severe pressure gradients is a necessary step toward developing useful actuators for gas turbine applications beyond laboratory use. The reported experiments were run at Reynolds numbers varying from 50,000 to 97,000, and utilizing various pulse frequencies. Further, the observed performance differences with varying electric field strengths are discussed for these Reynolds numbers. The results show that flow behaviors at high Reynolds numbers can be influenced by these types of actuators. The actuators were able to demonstrate a reduction in both wake width and momentum deficit.

The “Novel streaming potential (SP) and thermal sensor techniques for monitoring water and nutrient fluxes in the vadose zone” project ended Oct. 30, 2015, after an extension to complete travel and intellectual exchange of ideas and sensors. A significant component of this project was the development and testing of the Penta-needle Heat Pulse Probe (PHPP) in addition to testing of the streaming potential concept, both aimed at soil water flux determination. The PHPP was successfully completed and shown to provide soil water flux estimates down to 1 cm day⁻¹ with altered heat input and timing as well as use of larger heater needles. The PHPP was developed by Scott B. Jones at Utah State University with a plan to share sensors with Shmulik P. Friedman, the ARO collaborator. Delays in completion of the PHPP resulted in limited testing at USU and a late delivery of sensors (Sept. 2015) to Dr. Friedman. Two key aspects of the subsurface water flux sensor development that delayed the availability of the PHPP sensors were the addition of integrated electrical conductivity measurements (available in February 2015) and resolution of bugs in the microcontroller firmware (problems resolved in April 2015). Furthermore, testing of the streaming potential method with a wide variety of non-polarizable electrodes at both institutions was not successful as a practical measurement tool for water flux due to numerous sources of interference and the M.S. student in Israel terminated his program prematurely for personal reasons. In spite of these challenges, the project funded several undergraduate students building sensors and several master’s students and postdocs participating in theory and sensor development and testing. Four peer-reviewed journal articles have been published or submitted to date and six oral/poster presentations were also delivered by various authors associated with this project. We intend to continue testing the "new generation" PHPP probes at both USU and at the ARO resulting in several additional publications coming from this follow-on research. Furthermore, Jones is presently awaiting word on an internal grant application for commercialization of the PHPP at USU.

Research objectives were: 1) To study the effect of diet energy density, level of exercise, thermal conditions and reproductive state on cardiovascular function as it relates to oxygen (O2) mobilization. 2) To validate the use of heart rate (HR) to predict energy expenditure (EE) of ruminants, by measuring and calculating the energy balance components at different productive and reproductive states. 3) To validate the use of HR to identify changes in the metabolizable energy (ME) and ME intake (MEI) of grazing ruminants. Background: The development of an effective method for the measurement of EE is essential for understanding the management of both grazing and confined feedlot animals. The use of HR as a method of estimating EE in free-ranging large ruminants has been limited by the availability of suitable field monitoring equipment and by the absence of empirical understanding of the relationship between cardiac function and metabolic rate. Recent developments in microelectronics provide a good opportunity to use small HR devices to monitor free-range animals. The estimation of O2 uptake (VO2) of animals from their HR has to be based upon a consistent relationship between HR and VO2. The question as to whether, or to what extent, feeding level, environmental conditions and reproductive state affect such a relationship is still unanswered. Studies on the basic physiology of O2 mobilization (in USA) and field and feedlot-based investigations (in Israel) covered a , variety of conditions in order to investigate the possibilities of using HR to estimate EE. In USA the physiological studies conducted using animals with implanted flow probes, show that: I) although stroke volume decreases during intense exercise, VO2 per one heart beat per kgBW0.75 (O2 Pulse, O2P) actually increases and measurement of EE by HR and constant O2P may underestimate VO2unless the slope of the regression relating to heart rate and VO2 is also determined, 2) alterations in VO2 associated with the level of feeding and the effects of feeding itself have no effect on O2P, 3) both pregnancy and lactation may increase blood volume, especially lactation; but they have no effect on O2P, 4) ambient temperature in the range of 15 to 25°C in the resting animal has no effect on O2P, and 5) severe heat stress, induced by exercise, elevates body temperature to a sufficient extent that 14% of cardiac output may be required to dissipate the heat generated by exercise rather than for O2 transport. However, this is an unusual situation and its affect on EE estimation in a freely grazing animal, especially when heart rate is monitored over several days, is minor. In Israel three experiments were carried out in the hot summer to define changes in O2P attributable to changes in the time of day or In the heat load. The animals used were lambs and young calves in the growing phase and highly yielding dairy cows. In the growing animals the time of day, or the heat load, affected HR and VO2, but had no effect on O2P. On the other hand, the O2P measured in lactating cows was affected by the heat load; this is similar to the finding in the USA study of sheep. Energy balance trials were conducted to compare MEI recovery by the retained energy (RE) and by EE as measured by HR and O2P. The trial hypothesis was that if HR reliably estimated EE, the MEI proportion to (EE+RE) would not be significantly different from 1.0. Beef cows along a year of their reproductive cycle and growing lambs were used. The MEI recoveries of both trials were not significantly different from 1.0, 1.062+0.026 and 0.957+0.024 respectively. The cows' reproductive state did not affect the O2P, which is similar to the finding in the USA study. Pasture ME content and animal variables such as HR, VO2, O2P and EE of cows on grazing and in confinement were measured throughout three years under twenty-nine combinations of herbage quality and cows' reproductive state. In twelve grazing states, individual faecal output (FO) was measured and MEI was calculated. Regression analyses of the EE and RE dependent on MEI were highly significant (P<0.001). The predicted values of EE at zero intake (78 kcal/kgBW0.75), were similar to those estimated by NRC (1984). The EE at maintenance condition of the grazing cows (EE=MEI, 125 kcal/kgBW0.75) which are in the range of 96.1 to 125.5 as presented by NRC (1996 pp 6-7) for beef cows. Average daily HR and EE were significantly increased by lactation, P<0.001 and P<0.02 respectively. Grazing ME significantly increased HR and EE, P<0.001 and P<0.00l respectively. In contradiction to the finding in confined ewes and cows, the O2P of the grazing cows was significantly affected by the combined treatments (P<0.00l ); this effect was significantly related to the diet ME (P<0.00l ) and consequently to the MEI (P<0.03). Grazing significantly increased O2P compared to confinement. So, when EE of grazing animals during a certain season of the year is estimated using the HR method, the O2P must be re measured whenever grazing ME changes. A high correlation (R2>0.96) of group average EE and of HR dependency on MEI was also found in confined cows, which were fed six different diets and in growing lambs on three diets. In conclusion, the studies conducted in USA and in Israel investigated in depth the physiological mechanisms of cardiovascular and O2 mobilization, and went on to investigate a wide variety of ruminant species, ages, reproductive states, diets ME, time of intake and time of day, and compared these variables under grazing and confinement conditions. From these combined studies we can conclude that EE can be determined from HR measurements during several days, multiplied by O2P measured over a short period of time (10-15 min). The study showed that RE could be determined during the growing phase without slaughtering. In the near future the development microelectronic devices will enable wide use of the HR method to determine EE and energy balance. It will open new scopes of physiological and agricultural research with minimizes strain on animals. The method also has a high potential as a tool for herd management.