Дисертації з теми "Primary Human Tumor Cultures"
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Laryea, D., A. Isaksson, Colin W. Wright, R. Larsson, and P. Nygren. "Characterization of the cytotoxic activity of the indoloquinoline alkaloid cryptolepine in human tumour cell lines and primary cultures of tumour cells from patients." Springer, 2009. http://hdl.handle.net/10454/4533.
Повний текст джерелаThe plant derived indoloquinoline alkaloid cryptolepine was investigated for its cytotoxic properties in 12 human tumour cell lines and in primary cultures of tumour cells from patients. The fluorometric microculture cytotoxicity assay was used to assess cytotoxicity and DNA mocro-array analysis to evaluate gene expression. Cryptolepine mean IC50 in the cell line panel was 0.9 microM compared with 1.0 and 2.8 microM in haemaotological and solid tumour malignancies respectively. Among patient solid tumour samples, those from breast cancer were the most sensitive and essentially as sensitive as haematological malignancies, respectively. Among patient solid tumour samples, those from breast cancer were the most sensitive and essentially as senstive as haematological malignancies. Cryptolepine activity showed highest correlations to topoisomerase II and microtubule targetting drugs. In the cell lines cryptolepine activity was essentially unaffected by established mechanisms of drug resistance. A number of genes were identified as associated with cryptolepine activity. In conclusion, cryptolepine shows interesting in vitro cytotoxic properties and its further evaluation as an anticancer drug seems warranted.
Cunha, Virgínia Filipa Pereira Monteiro da. "Human adipose tissue primary cultures and impact in prostate cancer." Master's thesis, Universidade de Aveiro, 2009. http://hdl.handle.net/10773/8985.
Повний текст джерелаProstate cancer (Pca) is one of the most frequent diagnosed neoplasies and the second cause of cancer-related death in the world, in men. Between others risk factors, obesity has been associated to Pca although the innerent mechanisms to this association remain to be clear. With this work, throuhg in vitro studies, we wish to contribute to the understanding of the impact of white adipose tissue and its sub-fractions (adipocytes and stromal vascular fraction), from visceral and periprostatic anatomic regions, in celular proliferation, apoptosis and invasion of castration sensitivity (LNCaP) and castration resistant (PC-3) prostate cells. With the purpose of obtaining answers directly from humam studies, were performed visceral and periprostatic adipose tissue primary cultures obtained during urologic surgeries (radical prostatectomy and prostatic adenomectomy) (n=16). Adipose tissue was used to make primary organotipical cultures (WAT) and after collagenase digestion adipocytes and SVF primary cultures. Sobrenatants and infranatants of each culture were collect and used as conditioned medium representing adipokines production. LNCaP and PC-3 cell lines were stimulated with these mediums and apoptosis, proliferation and invasion were evaluated, in vitro. This study model represent a potential form for analyze the impact of adipose tissue in tumor cells, allowing to evaluate adipose tissue-tumor interactions. The results show that adipose tissue promotes tumor cells proliferation, that periprostatic adipose tissue increase apoptosis in obese individuals and that SVF subfraction suppresses invasion of PC-3 cells through a direct effect in tumor cells.
O Cancro da próstata (CaP) é uma das neoplasias mais frequentemente diagnosticadas e a segunda causa de morte por cancro no mundo nos homens. Entre outros factores de risco, a obesidade tem sido frequentemente associada a CaP, embora permaneçam por esclarecer os mecanismos subjacentes a esta associação. Com o presente trabalho pretendeu-se através de estudos in vitro, contribuir para a compreensão do impacto do tecido adiposo branco e suas sub-fracções (adipócitos e fracção vascular estromal), com origens anatómicas periprostática e viceral, na proliferação, apoptose, e invasão celular de células de cancro da próstata sensíveis à castração (LNCaP) e resistentes à castração (PC-3). Com o propósito de obter respostas directamente através de estudos em humanos, foram efectuadas culturas primárias de tecido adiposo periprostático e visceral obtido durante cirurgias urológicas (prostatectomia radical e adenomectomia prostática) (n=16). O tecido adiposo foi utilizado para realizar culturas primárias organotípicas (tecido adiposo total fraccionado) e após digestão com colagenase culturas primárias de adipócitos e de células da fracção vascular estromal do tecido adiposo. Foram colhidos sobrenadantes e infranadantes destas culturas de tecido adiposo e utilizados como meios condicionados representativos da produção de adipocinas. As linhas celulares LNCaP e PC-3 foram estimuladas com estes meios e avaliados a apoptose, proliferação celular e invasividade tumoral in vitro. Este modelo de estudo representa um potencial meio para análise do impacto do tecido adiposo nas células tumorais, permitindo avaliar as interacções tecido adiposo-tumor. Os resultados evidenciam que o tecido adiposo promove a proliferação das células tumorais, que o tecido adiposo periprostático aumenta a apoptose em indivíduos obesos e que os SVF suprimem a invasão das PC-3 através de um efeito directo nas células tumorais.
Ward, Rachel Ward. "Development of primary human keratinocyte cultures for studying chemical-induced skin irritation." Thesis, University of Nottingham, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241148.
Повний текст джерелаAl-Khalili, Lubna. "Gene regulation, intracellular signaling and membrane traffic : studies in primary human skeletal muscle cultures /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-866-1/.
Повний текст джерелаJohnson, Sara M. "Respiratory Syncytial Virus Uses CX3CR1 as a Cellular Receptor on Primary Human Airway Epithelial Cultures." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1448285474.
Повний текст джерелаIskander, Sammy. "Characterisation [i.e. characterization] of HIV-1 glycoprotein 120 neurotoxicity in primary human serum-free CNS cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0021/MQ58045.pdf.
Повний текст джерелаBroadbent, Lindsay Jane. "Exploitation of well-differentiated primary paediatric airway epithelial cell cultures (WD-PAECs) to study RSV/human host interactions." Thesis, Queen's University Belfast, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.695259.
Повний текст джерелаAndersson, Maria. "Chemically Induced DNA Damage in Extended-term Cultures of Human Lymphocytes." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7179.
Повний текст джерелаWong, Hing-ki Charmaine. "Viral determinants of influenza A (H5N1) associated TNF-a hyper-induction in human primary monocyte-derived macrophages." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37659029.
Повний текст джерелаWong, Hing-ki Charmaine, and 黃馨琦. "Viral determinants of influenza A (H5N1) associated TNF-a hyper-induction in human primary monocyte-derived macrophages." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37659029.
Повний текст джерелаMartínez, Romero Carles. "Polycomb group proteins Bmi1 and Ring1B are involved in cell plasticity and tumorigenesis of the pancreas." Doctoral thesis, Universitat Pompeu Fabra, 2009. http://hdl.handle.net/10803/7190.
Повний текст джерелаperò no en els acins, en el pàncrees adult. Bmi1 s'induí en cèl·lules acinars durant lesió aguda, en lesions metaplàstiques acinoductals, en neoplàsies intraepitelials pancreàtiques (PanIN) i en PDAC. Ring1B s'incrementà significativament en PanINs de grau alt i en PDAC. La disminució dels nivells de Bmi1 en la línia cel·lular acinar canvià l'expressió dels enzims digestius pancreàtics. Aquests resultats suggereixen que Bmi1 i Ring1B podrien estar contribuint de diferent manera en la progressió tumoral.
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers. To improve early diagnosis, research efforts are focused in characterising early events of cancer formation like preneoplastic lesions and deciphering the cell origin of the malignancy. Polycomb proteins constitute a family of epigenetic silencers found in a variety of solid tumours. The main hypothesis is that Polycomb might play a role in preneoplastic states in the pancreas and in tumour development and progression. The expression of Bmi1 and RingB was analysed during pancreatic development, in pancreatic tissue from mouse models of disease and in human pancreatic tissue samples. Mechanistic insights of Bmi1 were performed using in vitro models and with induced Bmi1 depletion. Bmi1 and Ring1B were expressed in pancreatic exocrine precursors during early development and in ductal and islet cells, but not in acinar cells, in the adult pancreas. Bmi1 was induced in acinar cells during acute injury, in acinar-ductal metaplastic lesions, in pancreatic intraepithelial neoplasia (PanIN) and PDAC. In contrast, Ring1B was significantly increased in high-grade PanINs and in PDAC. Bmi1 knockdown in acinar cell line changed the expression of pancreatic digestive enzymes. These results suggest that Bmi1 and Ring1B could contribute differently to tumour development.
Corry, Jacqueline D. "Prevention of Respiratory Syncytial Virus Attachment Protein Cleavage in Vero Cells Rescues Infectivity of Progeny Virions for Primary Human Airway Cultures." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449193388.
Повний текст джерелаMistry, Sanjay. "Differential expression of natriuretic peptide receptors in primary cultures of rat and human proximal tubular cells : a role for the natriuretic peptides." Thesis, University of Aberdeen, 1998. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU100279.
Повний текст джерелаCoulombel, Laure. "Hematopoiese dans les cultures a long-terme de moelle humaine normale et de moelle de malades atteints de leucemie myeloide chronique et leucemie aiguee myeloblastique." Paris 6, 1988. http://www.theses.fr/1988PA066170.
Повний текст джерелаBricks, Thibault. "Développement d’un dispositif microfluidique ayant pour objectif l’étude des effets de premiers passages intestinaux et hépatiques." Thesis, Compiègne, 2014. http://www.theses.fr/2014COMP2151/document.
Повний текст джерелаThe development of reliable and predictive in vitro methods is a real challenge. Indeed, the demand for alternative methods to animal experimentation has been growing in recent years due to the introduction of legislation limiting the use of these models in vivo by ethical considerations. Moreover, this need was amplified by regulations such as the European REACH (Registration, Evaluation, Authorization and Restriction of Chemicals) requiring the safety validation of many substances. However, the conventional in vitro model consisting in a simple cell culture monolayer in Petri dishes does not preserve the initial properties of these cells and does not mimic the conditions of the cellular environment and organs in vivo. The development of alternative in vitro predictive methods is crucial especially to mimic the working of two organs: the intestine and liver. Indeed, these two organs are involved in the process of Absorption, Distribution, Metabolism and Excretion (ADME) of most xenobiotics ingested.We propose in this thesis to test the feasibility of one of these in vitro alternative methods allowing the association between an intestinal barrier and the dynamic culture of hepatic cells in microsystems in a device called IIDMP (Integrated Dynamic Insert in a Microfluidic Platform). We tested the influence of the flow of culture and possible interactions between intestinal and liver cells on the function and metabolic activity of these two cell types.Then, we demonstrated that : - This device is reliable in terms of global functionality (fluid, robustness ...).- This device did not injury the integrity of the cell line and primary cells.- The use of this device has many advantages when compared with the use of conventional in vitro models, especially with cells line.- The use of this device highlights phenomena of interaction between hepatic and intestinal cells as an increase of the CYP1A2 activity of HepG2 C3A and human primary hepatocytes
Morales, Delphine. "Modèles 3D de mélanome métastatique pour l’évaluation in vitro de l’efficacité de molécules de thérapies ciblées." Thesis, Compiègne, 2019. http://www.theses.fr/2019COMP2498.
Повний текст джерелаMelanoma cell sensitivity to targeted therapy molecules is dependent on the tumor microenvironment (cell-cell and cell-extracellular matrix interactions). Three dimensional (3D) in vitro cell culture systems better reflect the native structural architecture of tissues and are attractive to investigate cellular interactions. We have developed and compared several metastatic melanoma models: melanoma cells (SK-MEL-28 and SK-MEL-3, BRAF V600E mutant and SK-MEL-2 BRAF wt) cultured as a monolayer (2D) and co-cultured on 3D dermal equivalents with fibroblasts to better unravel factors modulating cell sensitivity to a BRAF inhibitor (BRAFi, Vemurafenib) and a BRAFi combined with a MEK inhibitor (MEKi, Cobimetinib). Cell sensitivity to treatments was evaluated under various aspects: cell proliferation (cell counting, EdU incorporation, MTS assay), MAPK and PKB/Akt signaling pathway analysis (Western-blotting), apoptosis (TUNEL), cytokine and growth factor release (ELISA) and histology (3D models). A cytostatic effect of BRAFi was observed on SK-MEL-28 and SK-MEL-3 cells in both models. SK-MEL-2 cell line was clearly resistant to BRAFi when cultured as a monolayer but not when co-cultured with 3D fibroblasts embedded in a type I collagen matrix. Conditioned media provided by 3D fibroblasts (dermal equivalents) underlined 2D SK-MEL-2 sensitivity to BRAFi. Cell culture supernatant analysis revealed that dermal equivalents released some soluble factors (IL-6, IL-8, HGF, TGF-β): these secretions were modified during vemurafenib treatment. The combination of treatment with MEKi enhances the action of Vemurafenib on metastatic melanoma cells while decreasing the proliferation capacity of fibroblasts. Cell populations containing melanoma cells or fibroblasts associated with cancer (CAFs) were isolated from a cutaneous metastasis biopsy of a patient with metastatic melanoma. These cells allowed the realization of patient-specific models of metastatic melanoma in order to study in vitro the sensitivity of the patient’s melanoma cells to treatments in a tumor microenvironment (paracrine secretion of stromal cells and collagen matrix). These 3D predictive patient-specific models could be used to determine personalized therapy strategies, as well as to understand the resistance phenomena of melanoma cells to treatments
Joussain, Charles. "Construction and validation of HSV-1 vectors with selective and long-term expression in bladder afferent neurons for gene therapy of neurogenic detrusor overactivity. : A translational approach Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons Development and assessment of herpes simplex virus type 1 (HSV-1) amplicon vectors with expression from sensory neuron-selective promoters. Construction and properties of replication-incompetent HSV-1 recombinant vectors expressing transgenic botulinum toxins in primary cultures of human sensory neurons and displaying long-term expression in vivo. Therapeutic escalation for the neurogenic bladder in SCI patients : A bicentric study real life experience Long-term outcomes and risks factors for failure of intradetrusor onabotulinumtoxin A injections for the treatment of refractory neurogenic detrusor overactivity." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLV057.
Повний текст джерелаFifty to 80% of patients with traumatic spinal cord injury (SCI) undergo urinary incontinence episodes, mostly related to neurogenic detrusor overactivity (NDO). NDO is characterized by uninhibited detrusor contractions during the bladder-filling phase which could lead to a significant increase in bladder pressures, especially when associated to sphincter-destrusor-dyssynergia, leading to uro-nephrological complications. The main goal of NDO management following SCI is to achieve regular and complete bladder emptying, avoiding high intra-detrusor pressure and maintaining continence, in order to improve patients’ quality of life and to prevent renal failure. The current management is well characterized and relies on pharmacotherapy acting primarily at the level of efferent motor micturition reflex branch, thus allowing bladder filling at low pressure. First line treatment relies on oral antimuscarinics, often associated to clean intermittent bladder self-catheterization. When patients are refractory to antimuscarinics, injection of botulinum toxin A into the detrusor is proposed. However, despite their efficacy, these treatments fail to persist in the long term, leading to a third-line surgical treatment, which consists in cystoplasty augmentation or sacral neuromodulation. The Brindley technique, which consist in a sacral deafferentation of bladder posterior roots associated to an electrical stimulation, on demand, of anterior roots is a promising alternative, but remains seldom performed because of the complex surgical procedure required. NDO results from the emergence, secondary to neuronal plasticity following SCI, of an abnormal micturition reflex mediated by bladder afferent C-fibers, conveying aberrant sensory information to the spinal cord. The aim of the team where I developed my work is to silence these bladder afferent C-fibers in order to abolish the impaired spinal micturition reflex after SCI. In a second time, micturition would be fired, on demand, by electric stimulation of the bladder efferent neurons. My work consisted in developing the tools and methods required for such molecular deafferentation. Accordingly, I constructed replication-incompetent HSV-1 vectors conceived to deliver a therapeutic transcription cassette, consisting in the light chains of botulinum toxin (BoNT-LC) driven by the human version of the promoter of the gene encoding calcitonin gene-related protein (hCGRP), to achieve sensory neuron-selective transgenic expression. The transcription cassette was inserted into the LAT locus of the HSV-1 genome, the only region of the virus genome that remains transcriptionally active during latent infection. These vectors have been assessed (i) in vitro, on cell lines of neural origin and on primary cultures of rat embryonic and adult sensory neurons, and on primary cultures of adult human sensory and sympathetic neurons, (ii) ex vivo, on organotypic cultures of sensory, sympathetic and parasympathetic ganglia from adult rats, and (iii) in vivo, in sensory ganglia following infection at the hind footpad of adult rats.Our results indicate that (i) the vectors express functional BoNT-LC, thereby cleaving proteins of the SNARE complex in rat and human sensory neurons and inhibiting release of the neuromediator CGRP in rat sensory neurons, (ii) the transcription cassette delivered by the vectors display highly selectively expression towards human sensory neurons, as compared to human sympathetic neurons, and (iii) the vectors induced long-term transgenic expression in sensory (DRG) ganglia (at least for three months) following footpad injection. Therefore, the vectors seem to accomplish the three main specifications required for a future gene therapy strategy, allowing to restore urinary continence and micturition without catheterization and without any major surgery. This approach will represent a major breakthrough in the management of NDO in SCI patients with complete and incomplete lesion
Moussaud, Simon. "Etude de l'implication des cellules microgliales et de l'α-synucleine dans la maladie neurodégénérative de Parkinson". Phd thesis, Université de Bourgogne, 2011. http://tel.archives-ouvertes.fr/tel-00668186.
Повний текст джерелаTariq, S., M. Tahseen, M. Hassan, M. A. Masood, S. Khattak, A. A. Syed, A. H. Ahmad, M. Hussain, M. A. Yusuf, and Chris W. Sutton. "Stem Cell Organoids in Primary Cultures of Human Non-Malignant and Malignant Colon." 2017. http://hdl.handle.net/10454/13084.
Повний текст джерелаA sub-population of cells named cancer stem cells (CSCs) that initiate and promote tumour growth have been demonstrated to exist in several malignancies including colon carcinoma. The objective of our pilot study was to isolate CD133+CD26+CD44+ CSCs from patient colon tumours, culture spheres or organoids and observe their proliferation in primary cultures. Parallel cultures of non-cancer controls from colon normal lining and nonadenomatous polyps were set up. Magnetic activated cell sorting was used to isolate CD133+CD26+CD44+ cell populations followed by primary cell culturing under stem cell culture conditions. Number, cells/organoid and daughter generations of organoids were calculated using phase contrast microscope. Trypan blue exclusion method was used to test the viability of the cells. Both colon tumour and colon non-adenomatous polyp formed floating organoids in suspension; however non-adenomatous polyp cultures did not show self-renewal properties for more than 1 passage. Normal colon singlecell suspension did not create organoids. Metastatic colon tumours rapidly produce cancer cell organoids in less than 24 hours in larger numbers compared to non-metastatic colon tumours (1-3 weeks). Metastatic colon tumour organoids have the ability for proliferation for upto five daughter generations in primary culture compared to three generations for those grown from non-metastatic tumours. This in vitro CSC organoid model will help study colon cancer biology, in particular providing a valuable source of primary cell-derived tissue for studying personalized molecular profiling using ‘omics strategies to direct therapeutic intervention.
Xu, You-You, and 許優優. "Characterization of Tumor Suppressor Genes Rb and p16 in Human Esophageal Carcinoma Primary Tissues and Cell Lines." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/23099112545809113362.
Повний текст джерела台北醫學院
細胞及分子生物研究所
85
Esophageal Carcinoma is the tenth most common cancer in the Taiwanese population and the fifth most common cancer in the male population of Taiwan. Various genetic abnormalities have already been reported such as changes of tumor suppressor genes including p53, APC, MCC that genes are involved in esophageal carcinoma.The retinoblastoma gene product (pRB) is a nuclear phosphoprotein that is thought to play a key role in the negative regulation of cellular proliferation by modulating the activities of the transcriptional factors that control expression of S phase genes. D-type cyclins, in association with the cyclin-dependent kinases Cdk4 or Cdk6, promote progression through the G1 phase of the cell cycle by phosphorylating the pRB. The activities of Cdk4 and Cdk6 are constrained by inhibitors such as p16, the product of the p16 gene on human chromosome 9p21. The tumor suppressor gene p16(CDKN2/INK4A/MTS1) has been found to be deleted or mutated in a variety of human cancers. Thirteen human esophageal carcinoma primary tissue samples and five human esophageal carcinoma cell lines were analyzed for Rb gene alteration by single-strand conformational polymorphism (SSCP), DNA sequence analyses, and western blot assay. p16 gene alteration were detected by a method combining reverse transcription and nested polymerase chain reaction to detect different RNA transcripts of the p16 gene, associate with western blot assay to detect p16 protein expression, and polymerase chain reaction-methylation assay to detect the methylation status of genomic p16 gene in human esophageal carcinoma.The results showed that 2 of 13 esophageal carcinoma primary tissue samples and none of 5 esophageal carcinoma cell lines had altered Rb tumor suppressor gene; 3 of 10 primary tissue samples didn''t express pRb protein, including 1 primary tissue sample with altered Rb gene, but all of 5 cell lines express pRb protein. 8 of 13 primary tissues samples and all of 5 cell lines had altered p16 gene, 4 of 10 primary tissue samples and all of 5 cell lines fail to express p16 protein.Our result revealed that inactivation of the p16 tumor suppressor gene may play an important role in the development of esophageal carcinoma. But since the Rb tumor suppressor gene transcription factors binding sites alteration is not a frequent event in esophageal carcinoma, it will require more studies to understand Rb''s role in esophageal carcinoma.
Huang, Chung Guei, and 黃瓊瑰. "The Pilot Study on Primary Cultures of Human Respiratory Tract Epithelial Cells to Predict Patients’ Responses to H7N9 Infection." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/5797tx.
Повний текст джерелаSaleem, Saira, A. Jamshed, S. Faisal, R. Hussain, M. Tahseen, A. Loya, and Chris W. Sutton. "Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodes." 2014. http://hdl.handle.net/10454/9890.
Повний текст джерелаRecently a sub-population of cells with stem cell characteristics, reported to be associated with initiation, growth, spread and recurrence, has been identified in several solid tumors including oral tongue squamous cell carcinoma (OTSCC). The aim of our pilot study was to isolate CD44+ cancer stem cells from primary cultures of OTSCC and neck node Level I (node-I) biopsies, grow cell spheres and observe their characteristics in primary cultures. Parallel cultures of hyperplastic lesions of tongue (non-cancer) were set up as a control. Immunohistochemistry was used to detect CD44/CD24 expression and magnetic activated cell sorting to isolate CD44+ cell populations followed by primary cell culturing. Both OTSCC and node-I biopsies produced floating spheres in suspension, however those grown in hyperplastic and node-I primary cultures did not exhibit self-renewal properties. Lymph node metastatic OTSCC, express higher CD44/CD24 levels, produce cancer cell spheres in larger number and rapidly (24 hours) compared to node negative OTSCC (1 week) and non-cancer specimens (3 weeks). In addition, metastatic OTSCC have the capacity for proliferation for up to three generations in primary culture. This in vitro system will be used to study cancer stem cell behavior, therapeutic drug screening and optimization of radiation dose for elimination of resistant cancer cells.
SKMCH&RC, Yorkshire Cancer Research
Claudio, Jerome Anthony A. "The effects of cyclosporin A, tamoxifen and medroxyprogesterone acetate on the enhacement of adriamycin cytotoxicity in primary cultures of human breast epithelial cells." Thesis, 1995. http://hdl.handle.net/2429/4034.
Повний текст джерелаManduch, Zuzana Mercedes. "The effect of oxygen tension on 11beta-hydroxysteroid dehydrogenase type 2 (11betaHSD-2) expression and activity in primary and explant cultures of the human placenta." 2007. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=788915&T=F.
Повний текст джерелаLi, Yun Liao, and 廖麗雲. "Garlic oil compositions of 24 garlic lines cultivated in Taiwan and their effects on the cell viabilities of human liver tumor cell HepG2 and normal primary rat hepatocytes." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/26567716684609248421.
Повний текст джерела國立臺灣大學
食品科技研究所
91
Abstract The objectives of this study was to investigate the effects of twenty-four lines garlics included two kinds of type which were hard-neck and soft-neck from Taiwan. We extracted garlic oil (GO) by steam distillation method, and the average extraction were around 0.2%. The highest extraction (0.26%) was Chia-yi lines of hard-neck type. The components of twenty-four lines garlic oils were analyzed by GC-MS and used Cluster analysis method to analysis the five major organosulfur components (OSCs) which were diallyl sulfide (DAS), diallyl disulfide (DADS), methyl allyl disulfide (MADS), diallyl trisulfide (DATS), and methyl allyl trisulfide (MATS) to get four groups. The types of four groups were chosen hard-neck type of black leave, Chia-yi, white leave of Si-luo, and soft-neck type of An-nan flower to be sampling, and used the four samples to observe the effect of cell viability of the cell line HepG2 from human liver tumor cell and normal primary SD rat hepatocytes. The lowest IC50 effects of garlic oil concentraction for HepG2 from human liver tumor cell viability was black leave, and then were white leave of Si-luo and Chia-yi lines. The garlic oil from An-nan flower had the highest IC50 effects. The IC50 effects of garlic oil concentraction for normal primary SD rat hepatocytes, the garlic oil from white leave of Si-luo had the lowest effect, and then were black leave and Chia-yi lines. The garlic oil from An-nan flower had the highest IC50 effects. In a word garlic oil had lowet IC50 effects for HepG2 cell viability than normal primary SD rat hepatocytes.