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Статті в журналах з теми "Primary cell culture of bivalve"

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Автор: Grafiati

Опубліковано: 8 червня 2024

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1

Yoshino, T. P., U. Bickham, and C. J. Bayne. "Molluscan cells in culture: primary cell cultures and cell lines." Canadian Journal of Zoology 91, no. 6 (June 2013): 391–404. http://dx.doi.org/10.1139/cjz-2012-0258.

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata (Say, 1818) embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host – parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.

2

Potts, Robert W. A., Alejandro P. Gutierrez, Yennifer Cortés-Araya, Ross D. Houston, and Tim P. Bean. "Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas." PeerJ 8 (June 1, 2020): e9180. http://dx.doi.org/10.7717/peerj.9180.

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Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease.

3

Dessai, Shanti Nilesh. "Primary culture of mantle cells of bivalve mollusc, Paphia malabarica." In Vitro Cellular & Developmental Biology - Animal 48, no. 8 (August 8, 2012): 473–77. http://dx.doi.org/10.1007/s11626-012-9538-4.

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4

Odintsova, N. A., and A. V. Khomenko. "Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (Bivalvia)." Cytotechnology 6, no. 1 (May 1991): 49–54. http://dx.doi.org/10.1007/bf00353702.

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5

Morgan, Siân R., Laura Paletto, Benjamin Rumney, Farhana T. Malik, Nick White, Philip N. Lewis, Andrew R. Parker, Simon Holden, Keith M. Meek, and Julie Albon. "Establishment of long-term ostracod epidermal culture." In Vitro Cellular & Developmental Biology - Animal 56, no. 9 (October 2020): 760–72. http://dx.doi.org/10.1007/s11626-020-00508-8.

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Abstract Primary crustacean cell culture was introduced in the 1960s, but to date limited cell lines have been established. Skogsbergia lerneri is a myodocopid ostracod, which has a body enclosed within a thin, durable, transparent bivalved carapace, through which the eye can see. The epidermal layer lines the inner surface of the carapace and is responsible for carapace synthesis. The purpose of the present study was to develop an in vitro epidermal tissue and cell culture method for S. lerneri. First, an optimal environment for the viability of this epidermal tissue was ascertained, while maintaining its cell proliferative capacity. Next, a microdissection technique to remove the epidermal layer for explant culture was established and finally, a cell dissociation method for epidermal cell culture was determined. Maintenance of sterility, cell viability and proliferation were key throughout these processes. This novel approach for viable S. lerneri epidermal tissue and cell culture augments our understanding of crustacean cell biology and the complex biosynthesis of the ostracod carapace. In addition, these techniques have great potential in the fields of biomaterial manufacture, the military and fisheries, for example, in vitro toxicity testing.

6

Odintsova, Nelly A., Vyacheslav A. Dyachuk, and Leonid P. Nezlin. "Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)." Cell and Tissue Research 339, no. 3 (February 6, 2010): 625–37. http://dx.doi.org/10.1007/s00441-009-0918-3.

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7

Le Marrec-Croq, F., D. Glaise, C. Guguen-Guillouzo, C. Chesne, A. Guillouzo, V. Boulo, and G. Dorange. "Primary cultures of heart cells from the scallp Pecten maximus (mollusca-bivalvia)." In Vitro Cellular & Developmental Biology - Animal 35, no. 5 (May 1999): 289–95. http://dx.doi.org/10.1007/s11626-999-0073-x.

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8

Сhadaeva, А. А., O. S. Povolyaeva, and S. G. Yurkov. "Primary cell culture in virology." "Veterinary Medicine" Journal 23, no. 01 (January 2020): 51–54. http://dx.doi.org/10.30896/0042-4846.2020.23.1.51-54.

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9

Ren, Daan, and Joseph D. Miller. "Primary cell culture of suprachiasmatic nucleus." Brain Research Bulletin 61, no. 5 (September 2003): 547–53. http://dx.doi.org/10.1016/s0361-9230(03)00193-x.

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10

Dyachuk, Vyacheslav. "Extracellular matrix is required for muscle differentiation in primary cell cultures of larval Mytilus trossulus (Mollusca: Bivalvia)." Cytotechnology 65, no. 5 (May 9, 2013): 725–35. http://dx.doi.org/10.1007/s10616-013-9577-z.

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11

Saucedo, Pedro. "Primary culture of mantle cells of the pearl oyster Pinctada mazatlanica (Bivalvia: Pteriidae), with possible application to pearl farming." Hidrobiológica 29, no. 1 (April 30, 2019): 1–7. http://dx.doi.org/10.24275/uam/izt/dcbs/hidro/2019v29n1/saucedo.

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12

Hosseini Khorami, Hajar, Sophie Breton, and Annie Angers. "In vitro proliferation of Mytilus edulis male germ cell progenitors." PLOS ONE 19, no. 2 (February 9, 2024): e0292205. http://dx.doi.org/10.1371/journal.pone.0292205.

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Our understanding of basic cellular processes has mostly been provided by mammalian cell culture, and by some non-mammalian vertebrate and few invertebrate cell culture models. Developing reliable culture conditions for non-model organisms is essential to allow investigation of more unusual cellular processes. Here, we investigate how cells isolated from different tissues of the marine mussel Mytilus edulis thrive and survive in vitro in the hope of establishing a suitable laboratory model for the investigation of cellular mechanisms specific to these bivalve mollusks. We found that cells dissociated from mantle tissue attached to the culture vessels and proliferated well in vitro, whereas cells isolated from gills, although remaining viable, did not maintain divisions over three to four weeks in culture. We used antibodies against the germ-line marker DEAD-box helicase 4 (DDX4), also known as VASA, and the epithelial cell marker cytokeratin to distinguish different cell types in culture. DDX4-positive cells were predominant in 25-day-old cultures from male mantles. Cells from other tissues remained in low numbers and did not seem to change in composition over time. Overall, the culture conditions described here allow an efficient selection of male germ cells that could be used to study specific cellular mechanisms in vitro.

13

Mizumoto, Hiroyuki, Yuji Tomaru, Yoshitake Takao, Yoko Shirai, and Keizo Nagasaki. "Diverse Responses of the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama to Infection by a Single-Stranded RNA Virus." Applied and Environmental Microbiology 74, no. 10 (March 21, 2008): 3105–11. http://dx.doi.org/10.1128/aem.02190-07.

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ABSTRACT Viruses are believed to be significant pathogens for phytoplankton. Usually, they infect a single algal species, and often their infection is highly strain specific. However, the detailed molecular background of the strain specificity and its ecological significance have not been sufficiently understood. Here, we investigated the temporal changes in viral RNA accumulation and virus-induced cell lysis using a bloom-forming dinoflagellate Heterocapsa circularisquama and its single-stranded RNA virus, HcRNAV. We observed at least three host response patterns to virus inoculation: sensitive, resistant, and delayed lysis. In the sensitive response, the host cell culture was permissive for viral RNA replication and apparent cell lysis was observed; in contrast, resistant cell culture was nonpermissive for viral RNA replication and not lysed. In the delayed-lysis response, although viral RNA replication occurred, virus-induced cell lysis was faint and remarkably delayed. In addition, the number of infectious virus particles released to the culture supernatant at 12 days postinoculation was comparable to that of the sensitive strain. By further analysis, a few strains were characterized as variants of the delayed-lysis strain. These observations indicate that the response of H. circularisquama to HcRNAV infection is highly diverse.

14

De Rosa, Salvatore, Salvatore De Caro, Carmine Iodice, Giuseppina Tommonaro, Kamen Stefanov, and Simeon Popov. "Development in primary cell culture of demosponges." Journal of Biotechnology 100, no. 2 (January 2003): 119–25. http://dx.doi.org/10.1016/s0168-1656(02)00252-3.

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15

WATANABE, Seiki, Youichirou ISHIKAWA, Hiromi HARA, Kei HANZAWA, and Harutaka MUKOYAMA. "A Method of Primary Cell Culture for Establishing Equine Long-Term Culture Cell Lines." Journal of Equine Science 8, no. 4 (1997): 95–99. http://dx.doi.org/10.1294/jes.8.95.

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16

Blanco, Juan, Helena Martín, Carmen Mariño, and Araceli E. Rossignoli. "Simple Diffusion as the Mechanism of Okadaic Acid Uptake by the Mussel Digestive Gland." Toxins 11, no. 7 (July 6, 2019): 395. http://dx.doi.org/10.3390/toxins11070395.

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Okadaic acid (OA) and other toxins of the diarrheic shellfish poisoning (DSP) group are accumulated and transformed mainly in many bivalves, inside the digestive gland cells. In this work the absorption of okadaic acid by those cells has been studied by supplying the toxin dissolved in water and including it in oil droplets given to primary cell cultures, and by checking if the uptake is saturable and/or energy-dependent. Okadaic acid was found to be absorbed preferentially from the dissolved phase, and the uptake from oil droplets was substantially lower. The process did not require energy and was non-saturable, indicating that it involved a simple diffusion across the cellular membrane. Some apparent saturation was found due to the quick biotransformation of OA to 7-O-acyl esters.

17

Jeker, Lukas T., Mehrdad Hejazi, C. Lynne Burek, Noel R. Rose, and Patrizio Caturegli. "Mouse Thyroid Primary Culture." Biochemical and Biophysical Research Communications 257, no. 2 (April 1999): 511–15. http://dx.doi.org/10.1006/bbrc.1999.0468.

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18

Rajendra Prasad, Athe, T. K. Bhattacharya, R. N. Chatterjee, P. Guruvishnu, and N. Govardhana Sagar. "Standardization of Primary Hepatic Cell Culture in Chicken." International Journal of Current Microbiology and Applied Sciences 7, no. 05 (May 10, 2018): 80–82. http://dx.doi.org/10.20546/ijcmas.2018.705.011.

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19

Ikeda, Yuji, and Riichi Kusuda. "Studies on primary cell culture of eel leucocytes." NIPPON SUISAN GAKKAISHI 53, no. 4 (1987): 523–27. http://dx.doi.org/10.2331/suisan.53.523.

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20

LUO, SHULI, MEI SUN, RUI JIANG, GUAN WANG, and XINYI ZHANG. "Establishment of primary mouse lung adenocarcinoma cell culture." Oncology Letters 2, no. 4 (May 9, 2011): 629–32. http://dx.doi.org/10.3892/ol.2011.301.

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21

Ali, Akbar, and Donald L. Reynolds. "Primary Cell Culture of Turkey Intestinal Epithelial Cells." Avian Diseases 40, no. 1 (January 1996): 103. http://dx.doi.org/10.2307/1592378.

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22

Wang, Yanqiang, Wei Li, John E. Phay, Rulong Shen, Natalia S. Pellegata, Motoyasu Saji, Matthew D. Ringel, Albert de la Chapelle, and Huiling He. "Primary Cell Culture Systems for Human Thyroid Studies." Thyroid 26, no. 8 (August 2016): 1131–40. http://dx.doi.org/10.1089/thy.2015.0518.

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23

Sashikumar, Anu, and P. V. Desai. "Development of primary cell culture from Scylla serrata." Cytotechnology 56, no. 3 (March 2008): 161–69. http://dx.doi.org/10.1007/s10616-008-9152-1.

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24

Grabrucker, Andreas, Bianca Vaida, Jürgen Bockmann, and Tobias M. Boeckers. "Synaptogenesis of hippocampal neurons in primary cell culture." Cell and Tissue Research 338, no. 3 (November 3, 2009): 333–41. http://dx.doi.org/10.1007/s00441-009-0881-z.

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25

Akins, Robert E., Danielle Rockwood, Karyn G. Robinson, Daniel Sandusky, John Rabolt, and Christian Pizarro. "Three-Dimensional Culture Alters Primary Cardiac Cell Phenotype." Tissue Engineering Part A 16, no. 2 (February 2010): 629–41. http://dx.doi.org/10.1089/ten.tea.2009.0458.

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26

Kabgani, Nazanin, and Marcus J. Moeller. "The terminator mouse: salvation for primary cell culture." Kidney International 84, no. 5 (November 2013): 866–68. http://dx.doi.org/10.1038/ki.2013.288.

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27

FUKUZAWA, TOSHIHIKO, STEPHANIE J. HAMER, and JOSEPH T. BAGNARA. "Extracellular Matrix Constituents and Pigment Cell Expression in Primary Cell Culture." Pigment Cell Research 5, no. 5 (November 1992): 224–29. http://dx.doi.org/10.1111/j.1600-0749.1992.tb00541.x.

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28

Taketani, Y., and M. Mizuno. "Hormonal regulation of endometriotic cell growth in primary cell culture system." Archives of Gynecology and Obstetrics 251, no. 3 (September 1992): 127–32. http://dx.doi.org/10.1007/bf02718374.

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29

Witzmann, Frank A., James W. Clack, Kevin Geiss, Saber Hussain, Martha J. Juhl, Carol M. Rice, and Charles Wang. "Proteomic evaluation of cell preparation methods in primary hepatocyte cell culture." ELECTROPHORESIS 23, no. 14 (July 2002): 2223. http://dx.doi.org/10.1002/1522-2683(200207)23:14<2223::aid-elps2223>3.0.co;2-d.

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30

Gerlach, Jörg C., and Peter Neuhaus. "Culture model for primary hepatocytes." In Vitro Cellular & Developmental Biology - Animal 30, no. 10 (October 1994): 640–42. http://dx.doi.org/10.1007/bf02631264.

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31

KUBAT, B., G. REISS, and E. REALE. "Medullary collecting duct cells in primary culture." Cell Biology International Reports 14 (September 1990): 53. http://dx.doi.org/10.1016/0309-1651(90)90320-x.

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32

Karalyan, Zaven, Lusine Simonyan, Alla Misakyan, Liana Abroyan, Lina Hakobyan, Aida Avetisyan, and David Saroyan. "Cell Development in Primary Culture of Porcine Bone Marrow." CellBio 03, no. 02 (2014): 43–49. http://dx.doi.org/10.4236/cellbio.2014.32005.

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33

Hellermann, Gary R. "Book Review: A Manual for Primary Human Cell Culture." Cell Transplantation 14, no. 10 (November 2005): 859–60. http://dx.doi.org/10.3727/000000005783982585.

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34

Alamri, Ahmad M., Keunsoo Kang, Svenja Groeneveld, Weisheng Wang, Xiaogang Zhong, Bhaskar Kallakury, Lothar Hennighausen, Xuefeng Liu, and Priscilla A. Furth. "Primary cancer cell culture: mammary-optimized vs conditional reprogramming." Endocrine-Related Cancer 23, no. 7 (July 2016): 535–54. http://dx.doi.org/10.1530/erc-16-0071.

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The impact of different culture conditions on biology of primary cancer cells is not always addressed. Here, conditional reprogramming (CRC) was compared with mammary-optimized EpiCult-B (EpiC) for primary mammary epithelial cell isolation and propagation, allograft generation, and genome-wide transcriptional consequences using cancer and non-cancer mammary tissue from mice with different dosages of Brca1 and p53. Selective comparison to DMEM was included. Primary cultures were established with all three media, but CRC was most efficient for initial isolation (P<0.05). Allograft development was faster using cells grown in EpiC compared with CRC (P<0.05). Transcriptome comparison of paired CRC and EpiC cultures revealed 1700 differentially expressed genes by passage 20. CRC promoted Trp53 gene family upregulation and increased expression of epithelial differentiation genes, whereas EpiC elevated expression of epithelial–mesenchymal transition genes. Differences did not persist in allografts where both methods yielded allografts with relatively similar transcriptomes. Restricting passage (<7) reduced numbers of differentially expressed genes below 50. In conclusion, CRC was most efficient for initial cell isolation but EpiC was quicker for allograft generation. The extensive culture-specific gene expression patterns that emerged with longer passage could be limited by reducing passage number when both culture transcriptomes were equally similar to that of the primary tissue. Defining impact of culture condition and passage on the transcriptome of primary cells could assist experimental design and interpretation. For example, differences that appear with passage and culture condition are potentially exploitable for comparative studies targeting specific biological networks in different transcriptional environments.

35

Forrest, I. A. "Primary airway epithelial cell culture from lung transplant recipients." European Respiratory Journal 26, no. 6 (December 1, 2005): 1080–85. http://dx.doi.org/10.1183/09031936.05.00141404.

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36

CALKINS, J. H., M. M. SIGEL, H. R. NANKIN, and T. LIN. "Interleukin-1 Inhibits Leydig Cell Steroidogenesis in Primary Culture*." Endocrinology 123, no. 3 (September 1988): 1605–10. http://dx.doi.org/10.1210/endo-123-3-1605.

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37

Fernandes, M. N., F. B. Eddy, and W. S. Penrice. "Primary cell culture from gill explants of rainbow trout." Journal of Fish Biology 47, no. 4 (October 1995): 641–51. http://dx.doi.org/10.1111/j.1095-8649.1995.tb01931.x.

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38

Hagiwara, Yasuko, and Eijiro Ozawa. "Effects of hydroxyurea in primary skeletal muscle cell culture." Japanese Journal of Pharmacology 61 (1993): 203. http://dx.doi.org/10.1016/s0021-5198(19)51687-0.

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39

Sheridan, Robert E., Theresa J. Smith, and Michael Adler. "Primary cell culture for evaluation of botulinum neurotoxin antagonists." Toxicon 45, no. 3 (March 2005): 377–82. http://dx.doi.org/10.1016/j.toxicon.2004.11.009.

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40

Leung, K. W., Y. S. Chan, and K. K. L. Yung. "Dichlorodiphenyltrichloroethane Specifically Depletes Dopaminergic Neurons in Primary Cell Culture." Neuroembryology and Aging 2, no. 3 (2003): 95–102. http://dx.doi.org/10.1159/000074188.

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41

Yurdakök-Dikmen, Begüm, Pınar Arslan, Özgür Kuzukıran, Ayhan Filazi, and Figen Erkoç. "Unio sp. primary cell culture potential in ecotoxicology research." Toxin Reviews 37, no. 1 (May 29, 2017): 75–81. http://dx.doi.org/10.1080/15569543.2017.1331360.

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42

Kim, Sooah, Byung Woo Kim, Vicky P. Prizmic, Eugene Oh, Victoria Yu, Benjamin Evans, Dongwon Kim, and Luis A. Garza. "Simple cell culture media expansion of primary mouse keratinocytes." Journal of Dermatological Science 93, no. 2 (February 2019): 135–38. http://dx.doi.org/10.1016/j.jdermsci.2018.12.002.

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43

Ju, Hyunhee, and Sungho Ghil. "Primary cell culture method for the honeybee Apis mellifera." In Vitro Cellular & Developmental Biology - Animal 51, no. 9 (July 3, 2015): 890–93. http://dx.doi.org/10.1007/s11626-015-9924-9.

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44

Payushina, O. V., N. N. Butorina, O. N. Sheveleva, M. N. Kozhevnikova, and V. I. Starostin. "Cell Composition of the Primary Culture of Fetal Liver." Bulletin of Experimental Biology and Medicine 154, no. 4 (February 2013): 566–73. http://dx.doi.org/10.1007/s10517-013-2001-z.

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45

Walpita, Deepika, and Bridget K. Wagner. "Evaluation of Compounds in Primary Human Islet Cell Culture." Current Protocols in Chemical Biology 6, no. 3 (September 2014): 157–68. http://dx.doi.org/10.1002/9780470559277.ch140088.

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46

Michel, Olga, Piotr Błasiak, Jolanta Saczko, Julita Kulbacka, Małgorzata Drąg‐Zalesińska, and Adam Rzechonek. "Electropermeabilization of metastatic chondrosarcoma cells from primary cell culture." Biotechnology and Applied Biochemistry 66, no. 6 (September 18, 2019): 945–54. http://dx.doi.org/10.1002/bab.1809.

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47

He, Lingjie, Cheng Zhao, Qi Xiao, Ju Zhao, Haifeng Liu, Jun Jiang, and Quanquan Cao. "Profiling the Physiological Roles in Fish Primary Cell Culture." Biology 12, no. 12 (November 21, 2023): 1454. http://dx.doi.org/10.3390/biology12121454.

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Fish primary cell culture has emerged as a valuable tool for investigating the physiological roles and responses of various cell types found in fish species. This review aims to provide an overview of the advancements and applications of fish primary cell culture techniques, focusing on the profiling of physiological roles exhibited by fish cells in vitro. Fish primary cell culture involves the isolation and cultivation of cells directly derived from fish tissues, maintaining their functional characteristics and enabling researchers to study their behavior and responses under controlled conditions. Over the years, significant progress has been made in optimizing the culture conditions, establishing standardized protocols, and improving the characterization techniques for fish primary cell cultures. The review highlights the diverse cell types that have been successfully cultured from different fish species, including gonad cells, pituitary cells, muscle cells, hepatocytes, kidney and immune cells, adipocyte cells and myeloid cells, brain cells, primary fin cells, gill cells, and other cells. Each cell type exhibits distinct physiological functions, contributing to vital processes such as metabolism, tissue regeneration, immune response, and toxin metabolism. Furthermore, this paper explores the pivotal role of fish primary cell culture in elucidating the mechanisms underlying various physiological processes. Researchers have utilized fish primary cell cultures to study the effects of environmental factors, toxins, pathogens, and pharmaceutical compounds on cellular functions, providing valuable insights into fish health, disease pathogenesis, and drug development. The paper also discusses the application of fish primary cell cultures in aquaculture research, particularly in investigating fish growth, nutrition, reproduction, and stress responses. By mimicking the in vivo conditions in vitro, primary cell culture has proven instrumental in identifying key factors influencing fish health and performance, thereby contributing to the development of sustainable aquaculture practices.

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Bermudez-Lekerika, Paola, Katherine B. Crump, Karin Wuertz-Kozak, Christine L. Le Maitre, and Benjamin Gantenbein. "Sulfated Hydrogels as Primary Intervertebral Disc Cell Culture Systems." Gels 10, no. 5 (May 14, 2024): 330. http://dx.doi.org/10.3390/gels10050330.

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The negatively charged extracellular matrix plays a vital role in intervertebral disc tissues, providing specific cues for cell maintenance and tissue hydration. Unfortunately, suitable biomimetics for intervertebral disc regeneration are lacking. Here, sulfated alginate was investigated as a 3D culture material due to its similarity to the charged matrix of the intervertebral disc. Precursor solutions of standard alginate, or alginate with 0.1% or 0.2% degrees of sulfation, were mixed with primary human nucleus pulposus cells, cast, and cultured for 14 days. A 0.2% degree of sulfation resulted in significantly decreased cell density and viability after 7 days of culture. Furthermore, a sulfation-dependent decrease in DNA content and metabolic activity was evident after 14 days. Interestingly, no significant differences in cell density and viability were observed between surface and core regions for sulfated alginate, unlike in standard alginate, where the cell number was significantly higher in the core than in the surface region. Due to low cell numbers, phenotypic evaluation was not achieved in sulfated alginate biomaterial. Overall, standard alginate supported human NP cell growth and viability superior to sulfated alginate; however, future research on phenotypic properties is required to decipher the biological properties of sulfated alginate in intervertebral disc cells.

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Frank, H. G., O. Genbacev, A. Blaschitz, C. P. Chen, L. Clarson, D. Evain-Brion, L. Gardner, et al. "Cell Culture Models of Human Trophoblast—Primary Culture of Trophoblast—A Workshop Report." Placenta 21 (March 2000): S120—S122. http://dx.doi.org/10.1053/plac.1999.0528.

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Frank, H. G., D. W. Morrish, A. Pötgens, O. Genbacev, B. Kumpel, and I. Caniggia. "Cell Culture Models of Human Trophoblast: Primary Culture of Trophoblast—A Workshop Report." Placenta 22 (April 2001): S107—S109. http://dx.doi.org/10.1053/plac.2001.0644.

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