Дисертації з теми "Pre-implantation embryo"
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Graham, Sarah Jane Lehar. "Novel molecular mechanisms in pre-implantation mouse embryo development." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708487.
Повний текст джерелаWonnacott, Karen Elizabeth. "Fatty Acid Uptake in the Ruminant Ovary and Pre-Implantation Embryo." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503811.
Повний текст джерелаSpyropoulou, Isabella. "Studies of methods to improve human pre- and peri-implantation embryo development in vitro." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365394.
Повний текст джерелаKurowski, Agata Maria. "The establishment and maintenance of the pluripotency network in the pre-implantation mouse embryo." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.709248.
Повний текст джерелаBalasuriya, A. S. "An investigation of DNA integrity biomarkers in gametogenesis and pre-implantation embryo development to predict reproductive potential." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1416269/.
Повний текст джерелаHanna, Carol Bailey McCormick. "Comparison of gene expression in pre-implantation bovine embryos either injected or transfected with a siRNA targeted against E-cadherin." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-3048.
Повний текст джерелаHughes, Jaime. "The effect of dietary omega-3 and -6 polyunsaturated fatty acids on ovine ovarian function and the pre-implantation embryo." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/11800/.
Повний текст джерелаShaikly, Valerie Ruth. "An investigation into the factors associated with human pre-implantation embryo development and foetal maternal tolerance in the course of in vitro fertilization." Thesis, University of Essex, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502139.
Повний текст джерелаPorchet, Nicolas. "Role of signaling pathays in cell-fate specification in the early mouse embryo." Thesis, Université de Paris (2019-....), 2019. http://www.theses.fr/2019UNIP7096.
Повний текст джерелаDuring the early mouse embryogenesis, cell-fate specification events result in the formation of the pre-implantation blastocyst. Those events are mainly regulated by the action of signaling cascades activated upon fixation of the signaling molecules at the cell membrane. The activity of these signaling pathways allow the transcriptional regulation of a specific pool of genes responsible for cell-fate decisions and the formation of tissues. Here, I am interested in the roles of both ACTIVIN/NODAL and βCATENIN signaling pathways in the specification of cell identities during the maturation of the mouse blastocyst
Smith, Malcolm. "Regulating IVF and pre-implantation tissue-typing for the creation of "saviour siblings" : a harm analysis." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/35798/1/Malcolm_Smith_Thesis.pdf.
Повний текст джерелаCamozzato, Giovani Casanova. "Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/183449.
Повний текст джерелаThe early pregnancy of mare is a fascinating period that encompasses numerous and intense changes in its development, many of which are unique to the equine species. This development depends on the maintenance of the luteal function, the establishment of a favorable uterine environment and a precise and orchestrated interaction between the concept and the uterine environment. The aim of this study was to evaluate histological changes in the endometrium in days 7, 10 and 13 post-ovulation in pregnant and cyclic mares. In the first cycle, endometrial biopsies from 30 cyclic mares (Cyclic group) were collected on days 7, 10 and 13 post-ovulation. In the second cycle, the same mares were bred by a fertile stallion. At days 7, 10 and 13 post-ovulation intrauterine biopsies were collected. Immediately after sample collection, the mare‟s uteri were flushed, and those mares with embryo recovery were assigned to the Pregnant group. A larger blood vessel caliber was observed in pregnant mares than in cyclic from day 7 to 13. On the 7th day a large loss of ciliated cells was evident in the group of pregnant mares in comparison with the Cyclic group and the superficial cells of the endometrium were more protruded, and a small amount of histotrophic material between the folds was observed. On the 10th day of pregnancy, the glandular histotrophic secretion and the secretion of luminal epithelium became more intense than the secretion of cyclic mares. On the 13th day of pregnancy, a very large amount of histotroph was observed within large glandular openings surrounded by ciliated cells. Changes occurred in the uterine environment thereupon the entry of the embryo into the uterus. In the stroma and in the lumen, these modifications seem aim to provide the necessary nutrition for the initial development of the embryo and to promote changes at cellular structures that will interact in the embryonic signaling and future fixation, implantation and placentation.
Sun, Congshan. "Effect of pre-implantation maternal low protein diet on embryos and embryoid bodies." Thesis, University of Southampton, 2014. https://eprints.soton.ac.uk/363661/.
Повний текст джерелаMagaña, Griselda Valdez. "Crosstalk between embryonic and extraembyonic tissues in pre-implantation pig embryos." Thesis, University of Nottingham, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.662205.
Повний текст джерелаHenery, Caroline Cecilia. "The influence of ploidy on the pre- and post-implantation development of mouse embryos." Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/19837.
Повний текст джерелаFinch, Katie Anne. "Molecular Cytogenetic Studies of Human Sperm and Pre-Implantation Embryos with Specific Reference to Genome Organisation and Future Applications for Fertility Treatment and Diagnosis." Thesis, University of Kent, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499832.
Повний текст джерелаChin, Peck Yin. "Cytokines and programming the pre-implantation embryo." Thesis, 2014. http://hdl.handle.net/2440/92044.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Paediatrics and Reproductive Health, 2014
Tseng, Yu-Hsuan, and 曾于烜. "Effects of matrine on mouse pre-implantation embryo development." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/jbsdf7.
Повний текст джерела中原大學
奈米科技碩士學位學程
106
Matrine is a legume belonging to the dry roots of legumes extracted, in many studies found that matrine has many effects, such as: anti-arrhythmia, inhibition of cancer cell proliferation, anti-inflammatory effects and other effects. Many studies also found that matrine can regulate the function of leukopenia in the body, and also found that cound inhibit the proliferation and induce apoptosis in many cancer cell lines. Previous studies in our laboratory have found that co-culture of blastocysts with matrine inhibits the proliferation of embryonic cells, promotes apoptosis and affects DNA methylation. Study found that matrine increases H19 DNA methylation and induces high expression of IGF-2 gene, resulting in organ abnormalities and other effects. According the functional effects of matrine on inhibition of cell proliferation, induction of cell apoptosis, we further investigate the effects of matrin on early stage embryo development, including cell apoptosis, proliferation and in vitro embryonic development. The main purpose of this study was to investigate the effects of different concentrations of matrine (0, 12.5, 25, 50 μM) on the development of early stage embryos. Using the TUNEL assay, Hoechst staining analysis and in vitro development, studies found that the 12.5-50 μM matrin could inhibit cell proliferation and early embryonic development. Taken together, our study clearly point out that matrine has injury effects on cell proliferation and induce cell apoptosis, which can trigger developmental damage and cause embryo development injury. However, the detail regulatory mechanisms of matrin on early stage embryo development are unclear and need further investigation.
Chang, Chih-Chun, and 張智鈞. "Derivation and analysis of mouse pre-implantation embryo-specific RNF33-interacting proteins." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/38513077382281242675.
Повний текст джерела國立陽明大學
醫學生物技術研究所
95
RNF33, a mouse pre-implantation embryo-specific RING-finger protein, belongs to the TRIM/RBCC protein family. Each TRIM/RBCC protein is composed of a RING finger, one or two B-box fingers, a coiled-coil region and a carboxyl-terminal domain. Rnf33 transcripts are also detected in unfertilized eggs. To date, the biological functions of RNF33 in the mouse early embryo has not been determined. However, restrictive temporal expression and the highly conserved nature of RNF33 suggest that RNF33 should play an important role in pre-implantation embryonic development. In this study, we have identified three candidate RNF33-interacting proteins by screening of a yeast two-hybrid cDNA library. Expression of one of the candidate interacting protein genes, Kif3a, was detected in the egg and the pre-implantation stages by both bioinformatics analysis and direct PCR and Southern blot analysis. The KIF3A protein associates with KIF3B and KAP3 by the rod and the tail regions of the protein, respectively, to form a heterotrimeric complex which in turn acts as a motor protein for cellular transportation and cell division. Our experiments showed that the yeast two-hybrid system was not suitable for full-length KIF3A interaction tests. Hence, confirmation of interactions between full-length RNF33 and full-length KIF3A was demonstrated by an in vitro co-immunoprecipitation assay using baculovirus vector-expressed proteins. Interacting domains between RNF33 and KIF3A were further defined by domain mapping using the yeast two-hybrid system. The results showed that RNF33/KIF3A interaction occurs via the B30.2 domain of RNF33 and the coiled-coil rod region of KIF3A. Based on our data, we propose that the RNF33 may regulate KIF3A functions by blocking KIF3A/KIF3B heterodimeric complex formation, or, by interfering with the motility of the KIF3A/KIF3B/KAP3 heterotrimeric complex resulting in retarded cellular transportation or cell division, and critically affecting normal development.
Hung, Chi-Hsiang, and 洪啟翔. "Role of growth factor-induced epithelial-mesenchymal transition in embryo implantation and placentation: insight into implantation failure and pre-eclampcia." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/81075710184041786246.
Повний текст джерела國立陽明大學
解剖學及細胞生物學研究所
100
Implantation and placentation are critical precesses in pregnancy. During implantation, trophoblast increases the ability of migration and invasion to invade deeply enough into the endometrium in order to secure the embryo. Previous studies have shown that implantation failure and pre-eclampcia are caused by weak migration and invasion during implantation and placentation. Epithelial-mesenchymal transition (EMT) is a process of cell type changing from epithelial to mesenchymal type, during which the epithelial cells lose cell-cell junctions and cell-basal adhesions such that their ability of migration is increased and eventually these cells are transformed into mesenchymal cells at last. In this study, we hypothesize that the trophoblasts increase migratory ability during implantation and placentation through EMT. Experimentally, I first detected the expression of protein markers in human normal delivery placenta, and found that E-cadherin in cell column of choronic villi was decreased, and vimentin in extravillus cytotrophoblasts was increased. This suggests that EMT indeed occurs in trophoblast cells. Because previous studies have reported that myriad growth factors exist in the microenvironment of implantation to maintain the process of pregnancy. Therefore, I used mouse blastocysts to test 5 different growth factors for the ability to promote trophoblasts expansion as well as to trigger EMT in trophoblast stem (TS) cells. I found that EGF and FGF-II could enhance blastocyst expansion and trigger EMT on TS cells. On the other hand, I used an animal model to functionally inhibit uterus EGF and FGF-II receptors in mouse fertilized 3 ~ 3.5 days, and found that implantation rate was significantly reduced. When the uterus was treated with the EGFR inhibitor; PD153035; to expression of EMT protein markers, we found that E-cadherin expressed in giant cells but vimentin did not detected in giant cells. It imply that EMT could be inhibited by blocking EGF receptors. This study demonstrates that during embryo implantation and placentation, EGF-induced EMT promoted the increase in trophoblasts increasing migratory ability. Although FGF-II was also important to embryo implantation and placentation, but it had no effect on EMT. These results may be help to women suffering from low implantation rate.
Maserati, Marc P. Jr. "Characterization of Genes Required for Preimplantation Embryo Development." 2013. https://scholarworks.umass.edu/theses/1135.
Повний текст джерелаZander, Deirdre. "The impact of in vitro stress on pre-implantation embryo development, viability and mitochondrial homestasis." 2010. http://hdl.handle.net/2440/60987.
Повний текст джерелаhttp://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1522143
Thesis (Ph.D.) -- University of Adelaide, School of Paediatrics and Reproductive Health, 2010
MIHAJLOVIČ, Aleksandar. "The involvement of the Hippo signalling pathway in the first two cell-fate decisions of pre-implantation mouse embryo development." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-264795.
Повний текст джерелаChung, Yu Hsiang, and 鍾宇翔. "Pre-implantation Development of Mouse Embryos in Microwells." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/60023721727148669871.
Повний текст джерела國立清華大學
奈米工程與微系統研究所
103
Assisted reproductive technologies (ART) are of increasing importance and impact worldwide. A crucial aspect of ART is to control and mimic the in vitro environment of mammalian to the in vivo one. Integration biotechnology with micro- and nanotechnology is one of the necessary ways to improve ART. There are many platforms capable of nurturing cells in 3D, including using gel, microwells or hanging drop techniques. Microwells were chosen for the culture and investigation of early mouse embryos in this study, since microwells made of polydimethylsiloxane (PDMS) are low-cost, easy to operate, and biocompatible. Each mouse early embryo could be maintained in an individual microwell, allowing for high-resolution time-lapse microscopy and collecting the data of developmental process from every single embryo without confounding factors. In addition, the fluidic environment of each microwell could be secluded from each other by layering oil on top, preventing the communication of soluble factors between embryos cultured in individual microwells. The initial step of ART involves in vitro fertilization (IVF), and we demonstrated a successful fertilization rate of 67.9% in this research. We successfully cultured mouse embryos from the two-cell stage to blastocyst stage inside different volume of microwells with a ~80% successful rate. Among the five different volumes of microwells evaluated, we chose 393 nL microwells as the cultured platform to tracing the development process of mouse embryos. After cultured in microwells for 72 hours, 22 embryos at blastocyst stage were transferred into a recipient female mouse, and 15 mice were successfully born after 19 days. The development timings of mouse embryos that developed into blastocysts were statistically different to those of embryos that failed to form blastocysts (p–value < 10-10), and could be robust indicators of the quality of the embryo with >93% sensitivity and 100% specificity. This microwell platform, which supports the development of pre-implant embryos and is low-cost, easy to fabricate and operate, we believe, opens opportunities for a wide range of applications in reproductive medicine and cell biology.
Fu, Tzu-Yen, and 傅子彥. "Effects of angiogenin on the pre-implantation development of mouse embryos and establishment of an in vitro implantation model." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/56062218550924643632.
Повний текст джерела中興大學
動物科學系所
95
A group of homologous ribonucleases (RNases) belonged to the RNase A superfamily has been isolated and characterized from various species. During the past decades, a lot of efforts have been devoted to the study of RNases, including DNA cloning, analyses of their catalytic activities and structures. However, little is known about the biological functions of the ubiquitous RNases in vivo, especially RNase 5, the angiogenin. Recently, it was demonstrated that the gene expression could be blocked after injection of double-stranded RNA (dsRNA) into organisms and the phenomenon is called RNA interference (RNAi). The RNAi is mediated by 19-23 nucleotide dsRNAs homologous in sequence to the target genes to silence cognate genes post-transcriptionally, which involves mRNA degradation. Consequently, it shows the gene knockdown or knockdown-like effects. Hence, the aims of this study were to investigate the effects of angiogenin on the embryonic implantation by knocking down the expression of angiogenin (Ang) in mouse blastocysts, in addition to establishment of an in vitro culture system for early implantation study. In Experiment 1, the specific primers for Ang-1, Ang-2 and Ang-4 were designed to analyze the expression of pattern in mouse blastocysts by reverse transcription polymerase chain reaction (RT-PCR). The results showed that only Ang-1 was expressed at the blastocyst stage in mice. In Experiment 2, the different regions in the DNA sequences of Ang-1 and Ang-2 were selected and subcloned into RNAi expression vector, in which short hairpin RNAs were derived by U6 promoter. After transfected into B16-F10 mouse cell line, the expression of Ang-1 and Ang-2 were analyzed by RT-PCR. The RT-PCR results demonstrated that the expression of angiogenin was decreased to at least 40% by various RNAi expression vectors. In Experiment 3, the U6-sh-1 (RNAi expression cassette for knocking down Ang-1) or U6-shR-1 (RNAi expression cassette for knocking down RNase 1) fragments were injected into the pronuclei of mouse zygotes. The cleavage rates in the U6-sh-1-injected and U6-shR-1 injected groups were significantly lower than those in the uninjected control group after 72 h culture in vitro (77 % and 81% v.s. 95%, P < 0.05). No significant differences of morula/blastocyst formation were found between U6-sh-1-injected and U6-shR-1-injected groups (77% v.s. 81%, P > 0.05), but the development of morula/blastocyst in the U6-sh-1-injected or U6-shR-1 group was significantly decreased, compared to the control group (49% and 48% v.s. 90%, P < 0.05). The defective effects of U6-sh-1 and U6-shR-1 on the development of preimplantation embryos were demonstrated. Immunofluorescent staining showed that U6-sh-1 injection reduced Ang-1 protein levels in the blastocysts compared to those in the control group. In Experiment 4, the endometrial epithelial cells and stromal cells were isolated from mouse uteruses. For construction of the 3-dimensional culture system, epithelial cells were seeded on an artificial basal membrane (ECMatrix™) with underlying stromal cells embedded in the type I collagen matrix. The whole system was settled in a Millicell® (Millipore) hanging in a 24-well culture plate. The morphology of epithelial cells on the matrix became cuboidal after culture. Additionally, the columnar appearance with a basal nucleus was observed on the paraffin wax sections of epithelial cells. The mouse blastocysts were recovered and cultured in this model system. Normal hatching and attachment of the blastocyst were observed. The endometrial cells grown in the established in vitro culture system appeared similar morphology as those in vivo, suggesting this model system might facilitate further understanding of the cellular and molecular mechanisms involving in the implantation of mammalian embryos. In conclusion, the DNA-based shRNA constructs produced in this study could effectively decrease the expression of Ang-1. Further studies would be required to elicit the optimal culture conditions for the in vitro implantation model before applied to the study of the effect of angiogenin on the implantation.
Sarmiento, Micahella, and Micahella Sarmiento. "Study of nanoparticle influence on pre-implantation mammalian embryos: Perspective of spectroscopic quality analysis." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/f494n4.
Повний текст джерела國立東華大學
物理學系
107
Currently, many types of research are about the toxicity of nanoparticle (NP) on embryonic development considering NP’s various applications such as NP-containing products. NPs can possibly be released into the environment, interact with the human body, and even has the possibility to penetrate the reproductive system. Thus, this presented study aims to investigate the effect of nanoparticle (NP) on the developing embryo. By employing Raman Spectroscopy, this study will propose a method to evaluate the embryo quality before and after interaction with amine-functionalized nanodiamond (NDA). Spectroscopic analysis using Raman spectroscopy is presented as an additional method of estimation of the embryo by determining the embryo quality based on molecular information obtained which is non-invasive and under safe parameters for the live embryo. The Raman spectra of the embryo blastomeres have been measured using 785 nm NIR and 532 nm excitation wavelengths.
Caudle, Jeffrey. "Examination of Y Chromosome Linked Gene Expression in Healthy and Arrested Pre-implantation Bovine Embryos." Thesis, 2012. http://hdl.handle.net/10214/7735.
Повний текст джерела