Добірка наукової літератури з теми "Porirua"
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Статті в журналах з теми "Porirua"
Taankink, Jasmine, and Hugo Robinson. "Dispossession and Gentrification in the Porirua Redevelopment." Counterfutures 9 (March 7, 2021): 97–120. http://dx.doi.org/10.26686/cf.v9.6776.
Повний текст джерелаRoach, Elyjana. "Driving Through the East." Counterfutures 9 (March 7, 2021): 137–43. http://dx.doi.org/10.26686/cf.v9.6778.
Повний текст джерелаGlasby, G. P., R. L. Moss, and P. Stoppers. "Heavy‐metal pollution in Porirua Harbour, New Zealand." New Zealand Journal of Marine and Freshwater Research 24, no. 2 (June 1990): 233–37. http://dx.doi.org/10.1080/00288330.1990.9516419.
Повний текст джерелаDellow, G. D., S. A. L. Read, J. G. Begg, R. J. Van Dissen, and N. D. Perrin. "Distribution of geological materials in Lower Hutt and Porirua, New Zealand." Bulletin of the New Zealand Society for Earthquake Engineering 25, no. 4 (December 31, 1992): 332–44. http://dx.doi.org/10.5459/bnzsee.25.4.332-344.
Повний текст джерелаStephenson, W. R., and P. R. Barker. "Evaluation of sediment properties in the Lower Hutt and Porirua areas by means of cone and seismic cone penetration tests." Bulletin of the New Zealand Society for Earthquake Engineering 25, no. 4 (December 31, 1992): 265–85. http://dx.doi.org/10.5459/bnzsee.25.4.265-285.
Повний текст джерелаJones, J. B., and J. D. Hadfield. "Fishes from Porirua and Pauatahanui inlets: Occurrence in gill nets." New Zealand Journal of Marine and Freshwater Research 19, no. 4 (December 1985): 477–84. http://dx.doi.org/10.1080/00288330.1985.9516112.
Повний текст джерелаVan Dissen, R. J., J. J. Taber, W. R. Stephenson, S. Sritheran, S. A. L. Read, G. H. McVerry, G. D. Dellow, and P. R. Barker. "Earthquake ground shaking hazard assessment for the Lower Hutt and Porirua areas, New Zealand." Bulletin of the New Zealand Society for Earthquake Engineering 25, no. 4 (December 31, 1992): 286–302. http://dx.doi.org/10.5459/bnzsee.25.4.286-302.
Повний текст джерелаTaber, J. John, and Euan G. C. Smith. "Frequency dependent amplification of weak ground motions in Porirua and Lower Hutt, New Zealand." Bulletin of the New Zealand Society for Earthquake Engineering 25, no. 4 (December 31, 1992): 303–31. http://dx.doi.org/10.5459/bnzsee.25.4.303-331.
Повний текст джерелаEiby, George. "Changes to Porirua Harbour in about 1855 : historical tradition and geological evidence." Journal of the Royal Society of New Zealand 20, no. 2 (June 1990): 233–48. http://dx.doi.org/10.1080/03036758.1990.10426727.
Повний текст джерелаFairley, Nigel A., Richard Siegert, Alexander Simpson, Janice Wilson, and Brendan Roach. "Psychiatric Disorder and Disability in New Zealand Long-Stay Psychiatric Patients." Australian & New Zealand Journal of Psychiatry 27, no. 4 (December 1993): 590–94. http://dx.doi.org/10.3109/00048679309075820.
Повний текст джерелаДисертації з теми "Porirua"
Pereira, Andrea dos Santos [UNIFESP]. "Determinação da concentração de antimicrobiano capaz de prevenir o aparecimento de mutantes resistentes em amostras clínicas de acinetobacter spp." Universidade Federal de São Paulo (UNIFESP), 2009. http://repositorio.unifesp.br/handle/11600/39347.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
FAPESP: 04/14434-3
Milan, Arlete 1972. "Estudo epidemiologico-molecular e da resistencia ao imipenem, ocasionda pela perda de canais de porina, em Pseudomonas aeruginosa isolada de pacientes hospitalizados." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310673.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Introdução: A Pseudomonas aeruginosa é um importante agente de infecção nosocomial e motivo de grande preocupação por apresentar, com freqüência, resistência a diversos antimicrobianos. Os principais mecanismos de resistência encontrados são: inativação por enzimas, alterações na permeabilidade da membrana celular e promoção de efluxo. O conhecimento dos mecanismos de resistência que operam nesses microrganismos, bem como o estudo epidemiológico-molecular desses isolados, pode contribuir para a melhor compreensão e conseqüente controle dessas infecções no ambiente hospitalar. Objetivos: Determinar a Concentração Inibitória Mínima do Imipenem de Pseudomonas aeruginosa resistentes a esse antimicrobiano, isoladas de pacientes internados em hospital; Determinar a frequência de produção de metalo-beta-lactamases e da expressão da porina Opr D2 de Pseudomonas aeruginosa resistentes ao Imipenem, isoladas de pacientes internados em hospital; Genotipar e estudar a relação genética de Pseudomonas aeruginosa resistentes ao Imipenem, isoladas de pacientes internados em hospital. Materiais e Métodos: Foram estudadas 138 cepas isoladas de diversas amostras clínicas, identificadas bioquimicamente por técnicas de rotina e por automação. A triagem para a detecção de amostras produtoras de MBL's foi realizada pela fita de Etest combinada com EDTA. A extração e eletroforese de proteínas da parede celular foi executada para avaliar a expressão de porina Opr D2. Para a genotipagem foi utilizada a técnica de PFGE. Resultados e Discussões: Foram estudados 138 isolados, encaminhados como resistentes ao Imipenem. Em 128 desses isolados a resistência foi confirmada através do teste de difusão com disco e com a determinação do MIC (n = 98), através do Etest. A pesquisa da porina Opr D2 foi realizada em todos os isolados resistentes, ou seja, 128 casos. Em 68 deles não havia a expressão dessa proteína. Quando combinados os resultados da pesquisa de metalo-beta-lactamases e da deleção da porina Opr D2, encontramos 24 isolados que exibiam ambos os mecanismos de resistência. Da análise genotípica de 117 isolados, mostrou um acentuado polimorfismo, mesmo em isolados obtidos em um mesmo paciente. Não foram caracterizados surtos. Conclusões: A maioria das cepas de Pseudomonas aeruginosa resistentes ao Imipenem, isoladas em ambiente hospitalar, possui elevada resistência; A produção de meta-lobeta-lactamases constitui um mecanismo de resistência importante nesses isolados; A deleção da porina Opr D2 de membrana celular foi observada na maioria dos isolados. Em cerca de um terço deles havia também produção de metalo-beta-lactamase; A conglomeração desses isolados foi baixa, não caracterizando surtos em todos os casos; A variabilidade genotípica dos isolados foi intensa, mesmo em um determinado paciente, indicando que as ações relativas ao controle da disseminação desse patógeno deve ser de natureza rotineira, com as precauções de contato de universais e com o controle do uso de antimicrobianos
Abstract: Objective: Metallo-ß-lactamase production and Opr D2 channels expression was investigated in Imipenem resistant Pseudomonas aeruginosa recovered from hospital acquired infections. Design: Descriptive study in a convenient sample of organisms. Setting: Two 400-bed general teaching hospital, both tertiary care teaching hospitals, in Campinas, Brazil. All Imipenem resistant P. aeruginosa, recovered from March, 2000 through December 2004 from hospitalized patients were collected. Methods: Disk diffusion tests were used to confirm Imipenem resistance. Etest MBL was done to check for MBL production, and. Imipenem MIC's. Opr D2 expression was checked using cellular membrane proteins electrophoresis PFGE-genotyping was done in all isolates. Results: A sample of 128 Imipenem resistant Pseudomonas aeruginosa isolates was collected during the study period. Most isolates exhibited Imipenem MIC's = 256 µg / mL. Macrorestriction analysis (SpeI) using pulsed field gel electrophoresis (PFGE) showed a substantial polymorphism. Only 15 strains could be allocated to seven clusters, six with two isolates and one with three isolates. Ninety-nine Imipenem resistant isolates were screened for MBL production, and 87 were screened for both MBL, and porin Opr D2 expression. Sixty four isolates showed MBL production. Conclusion: Dissemination of MBL producing-genotypically heterogenous Pseudomonas aeruginosa strains was documented in the hospitals studied. Lack of Opr D2 combined with MBL production contributed to the high imipenem-resistance rates observed
Mestrado
Ciencias Basicas
Mestre em Clinica Medica
Muhizi, Théoneste. "Synthèse d’aminosucres conduisant à des biocides d’origine naturelle." Thesis, Bordeaux 1, 2008. http://www.theses.fr/2008BOR13641/document.
Повний текст джерелаIn this study different glucosylamines and amino desoxyglucoses were synthesized and characterised using various spectroscopic methods including IRFT, both 1H and 13C NMR spectroscopy and MALDI-Tof MS. Biological assessment of these compounds realised with two wood decay fungi, Coriolus versicolor and Poria placenta on one hand, and with three food microorganisms Listeria innocua, Salmonella typhimurium and Fusarium proliferatum on other hand, indicated a positive impact of both N-alkylation and degree of N-substitution and quaternisation on their growth inhibition. Furthermore, a biological impact of the amine position on sugar was studied. It was found that amine function attached to the C-1 of glucose conducted to the best antifungal activity against both C. versicolor and P. placenta while that fixed on the C-3 of glucose was indicated for antibacterial activity against L. innocua and S. typhimurium
Andrade, Meire Cristina Nogueira de [UNESP]. "Controle de fungos contaminantes no cultivo do cogumelo comestível shiitake (Lentinula edodes) em toros de eucalipto (Eucalyptus urophylla)." Universidade Estadual Paulista (UNESP), 2003. http://hdl.handle.net/11449/98870.
Повний текст джерелаFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A ocorrência de fungos contaminantes e competidores é comum durante o cultivo de shiitake em toros. No Brasil, poucos são os trabalhos que identificam e analisam os efeitos destes microrganismos na produção de shiitake. No entanto, sabe-se que a incidência destes fungos em grandes proporções pode levar à improdutividade dos toros. Portanto, o presente trabalho teve como objetivo testar o efeito da cal hidratada e do fungicida benomyl no controle de fungos contaminantes e sua resposta na produção de shiitake em toros. Para tanto, testou-se anteriormente in vitro o efeito do benomyl nas concentrações 0,5; 1,0; 2,0; 4,0; 8,0 e 16,0 mg/mL no crescimento micelial das linhagens de shiitake JAB-L; JAB-K; LE-96/17; LE-95/01 e LE-96/22, de modo a selecionar a linhagem mais tolerante as concentrações de benomyl propostas, comparando com o crescimento das mesmas sem a presença deste fungicida (testemunha). O delineamento deste experimento foi inteiramente casualizado em fatorial 5X7, tendo no total trinta e cinco tratamentos, cada qual com três repetições, sendo cada repetição correspondente a uma placa de Petri. Observou-se que a única linhagem de shiitake a não sofrer qualquer efeito do benomyl nas concentrações propostas foi a LE-96/17, sendo a escolhida para ser utilizada no experimento em toros, a qual foi submetida a maior concentração de benomyl (16,0 mg/mL). O delineamento do experimento em toros foi inteiramente casualizado, com três tratamentos: testemunha; cal (8 kg de cal/ 60 litros de água) aplicados nos toros de produção logo após a inoculação e após cada choque de indução; e benomyl (16,0 mg/mL) aplicados quinzenalmente a partir da inoculação dos toros. Cada tratamento conteve 60 repetições, sendo a unidade experimental correspondente a um toro. Os toros utilizados foram de Eucalyptus urophylla, os quais foram inoculados... .
Andrade, Meire Cristina Nogueira de 1978. "Controle de fungos contaminantes no cultivo do cogumelo comestível shiitake (Lentinula edodes) em toros de eucalipto (Eucalyptus urophylla) /." Ilha Solteira : [s.n.], 2003. http://hdl.handle.net/11449/98870.
Повний текст джерелаBanca: Luzia Doretto Paccola Meirelles
Banca: Marli de Fátima Stradioto Papa
Abstract: The occurrence of contaminants fungus and competitors are common during the shiitake cultivation in logs. In Brazil, few are the works that identify and they analyze the effects of these microorganisms in the shiitake production. However, it is known that the incidence of these fungus in great proportions can take to the unproductiveness the logs. Therefore, the present work had as objective to test the effect of the moisturized whitewash and of the fungicide benomyl in the control of contaminant fungus and his answer in the shiitake production in logs. For so much, it was tested in vitro previously the effect of the benomyl in different concentrations (0.5; 1.0; 2.0; 4.0; 8.0 and 16.0 mg/mL) in the mycelial growth of five shiitake lineages (JAB-L; JAB-K; LE-96/17; LE-95/01 and, LE-96/22), in way to select the most tolerant lineage to the concentrations of proposed benomyl, comparing with the growth of the same ones without the presence of this fungicide (control). A completely randomized design in a factorial scheme 5X7, tends in the total 35 treatments, each one with three repetitions, corresponding to a plate of Petri. It was observed that only shiitake lineage did not suffer any effect of the benomyl in the proposed concentrations was the LE-96/17, being the chosen to be used in the experiment in logs, when the largest benomyl concentration was submitted (16.0 mg/mL). The other study was conducted in logs in an interely randomized design, with three treatments: control; whitewash (8 kg of whitewash / 60 liters of water) applied in the production logs soon after the inoculation and after each induction shock; and benomyl (16.0 mg/mL) applied biweekly starting from the inoculation of the logs. Each treatment contained 60 repetitions, being the experimental unit corresponding to a log. The used logs were of Eucalyptus urophylla, which were... (Complete abstract, click electronic address below).
Resumo: A ocorrência de fungos contaminantes e competidores é comum durante o cultivo de shiitake em toros. No Brasil, poucos são os trabalhos que identificam e analisam os efeitos destes microrganismos na produção de shiitake. No entanto, sabe-se que a incidência destes fungos em grandes proporções pode levar à improdutividade dos toros. Portanto, o presente trabalho teve como objetivo testar o efeito da cal hidratada e do fungicida benomyl no controle de fungos contaminantes e sua resposta na produção de shiitake em toros. Para tanto, testou-se anteriormente in vitro o efeito do benomyl nas concentrações 0,5; 1,0; 2,0; 4,0; 8,0 e 16,0 mg/mL no crescimento micelial das linhagens de shiitake JAB-L; JAB-K; LE-96/17; LE-95/01 e LE-96/22, de modo a selecionar a linhagem mais tolerante as concentrações de benomyl propostas, comparando com o crescimento das mesmas sem a presença deste fungicida (testemunha). O delineamento deste experimento foi inteiramente casualizado em fatorial 5X7, tendo no total trinta e cinco tratamentos, cada qual com três repetições, sendo cada repetição correspondente a uma placa de Petri. Observou-se que a única linhagem de shiitake a não sofrer qualquer efeito do benomyl nas concentrações propostas foi a LE-96/17, sendo a escolhida para ser utilizada no experimento em toros, a qual foi submetida a maior concentração de benomyl (16,0 mg/mL). O delineamento do experimento em toros foi inteiramente casualizado, com três tratamentos: testemunha; cal (8 kg de cal/ 60 litros de água) aplicados nos toros de produção logo após a inoculação e após cada choque de indução; e benomyl (16,0 mg/mL) aplicados quinzenalmente a partir da inoculação dos toros. Cada tratamento conteve 60 repetições, sendo a unidade experimental correspondente a um toro. Os toros utilizados foram de Eucalyptus urophylla, os quais foram inoculados... (Resumo completo, clicar acesso eletrônico abaixo).
Mestre
Dias, Vanessa Cordeiro. "Resistência aos carbapenêmicos e virulência de Acinetobacter baumannii e Pseudomonas aeruginosa isolados de um serviço de saúde terciário." Universidade Federal de Juiz de Fora, 2015. https://repositorio.ufjf.br/jspui/handle/ufjf/1385.
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FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
As bactérias Gram-negativas não fermentadoras, como Pseudomonas aeruginosa e Acinetobacter baumannii, estão amplamente disseminadas no ambiente e estão cada vez mais associados a infecções nosocomiais graves. O uso extensivo e indiscriminado de antibióticos tem contribuído para um aumento do número de infecções causadas por A. baumannii e P. aeruginosa resistentes a uma grande variedade de agentes antimicrobianos, incluindo β-lactâmicos. O objetivo deste trabalho foi avaliar características fisiológicas e moleculares da resistência aos carbapenêmicos em P. aeruginosa e A. baumannii isolados em um hospital terciário. A partir de estudos com amostras clínicas de pacientes admitidos num hospital terciário foram isolados, em 2012, A. baumannii (n=44) e P. aeruginosa (n=28) resistentes aos carbapenêmicos e em 2013, P. aeruginosa (n=19) e A. baumannii (n=44) com igual fenótipo. As amostras bacterianas recuperadas em 2012 foram submetidas a testes de susceptibilidade aos antimicrobianos. Marcadores genéticos relacionados com a síntese de β-lactamases blaOXA-23, blaOXA-24, blaOXA-51, blaOXA-58 e blaOXA-143 foram testados por PCR. A partir das linhagens isoladas no estudo de 2013, testes fisiológicos foram realizados para avaliar a atividade hemolítica, estresse oxidativo, tolerância aos biocidas, formação de biofilme e determinação da resistência aos antimicrobianos. Marcadores genéticos relacionados com a síntese de β-lactamases (ampC, blaKPC, blaSIM, blaIMP, blaSPM-1, blaVIM, blaGIM, blaOXA e blaNDM-1), sistemas de efluxo (adeB, mexB, mexD, mexF e mexY) e perda de porina (oprD) foram pesquisados por PCR. Foram analisados dados epidemiológicos de todos os pacientes avaliados. No estudo de 2012, a polimixina B foi a única droga eficaz para todos os isolados. Os marcadores genéticos foram observados apenas em isolados de Acinetobacter. O genótipo mais frequente observado foi blaOXA-23+/blaOXA-51+ (45,5%), seguido por blaOXA-51+/blaOXA-143+ (41%). Os genes blaOXA-24 e blaOXA-58 não foram detectados. Uma elevada taxa de mortalidade foi observada (> 70%) entre os pacientes. No estudo de 2013, idade avançada, predomínio de indivíduos internados em UTI, uso de dispositivos médicos invasivos, como cateter venoso, tratamento anterior com fluoroquinolonas ou ß-lactâmicos em combinação com um inibidor da β-lactamase e estadia prolongada no hospital foram fatores predisponentes para infecção por estes microrganismos. Colistina demonstrou atividade, in vitro, contra todas as amostras bacterianas avaliadas. Tigeciclina foi também efetiva para linhagens de A. baumannii. P. aeruginosa resistente aos carbapenêmicos não foi capaz de produzir hemolisinas. Essas linhagens foram menos tolerantes ao estresse oxidativo e alguns biocidas, mas mostraram um aumento da capacidade de formação de biofilme em relação aos isolados sensíveis. Os principais mecanismos de resistência presentes em linhagens de A. baumannii resistentes aos carbapenêmicos foi síntese de oxacilinases (OXA-23, OXA-51 e OXA-143), ausência de oprD e presença de bomba de efluxo (AdeABC). Em P. aeruginosa foram encontrados genes para bombas de efluxo (MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY-OprM), blaSPM-1, além de ausência de oprD. Estes resultados confirmam a dificuldade de manejo terapêutico de pacientes com infecções associadas a microrganismos multirresistentes, com impacto direto na mortalidade e controle epidemiológico dessas linhagens nos centros de saúde.
The non-fermenting Gram-negative bacteria, such as Pseudomonas aeruginosa and Acinetobacter baumannii are widespread in the environment and are increasingly associated with severe nosocomial infections. Extensive and indiscriminate use of antibiotics has contributed to an increased number of infections caused by A. baumannii and P. aeruginosa that are resistant to a wide variety of antimicrobials, including β-lactams. This study aimed to evaluate physiological and molecular characteristics of carbapenem resistance in P. aeruginosa and A. baumannii. From studies with clinical samples from patients admitted to a tertiary hospital, were isolated in 2012 A. baumannii (n=44) and P. aeruginosa (n=28) resistant to carbapenems and in 2013, P. aeruginosa (n=19) and A. baumannii (n=44) with similar phenotype. The bacterial samples recovered in 2012 were submitted to antibiotic susceptibility testing. Genetic markers related to β-lactamases synthesis blaOXA-23, blaOXA-24, blaOXA-51, blaOXA-58 and blaOXA-143 were screened by PCR. From strains recovered in the 2013 study, physiological tests were performed to evaluate hemolytic activity, oxidative stress, biocides tolerance and biofilm formation, besides determination of antimicrobial resistance patterns. Genetic markers related to β-lactamases synthesis (ampC, blaKPC, blaSIM, blaIMP, blaSPM-1, blaVIM, blaGIM, blaOXA and blaNDM-1), efflux systems (adeB, mexB, mexD, mexF and mexY) and loss of porin (oprD) were screened by PCR. Epidemiological data about all of these patients were analyzed. In the 2012 study, polymyxin B was the only effective drug for all isolates. Genetic markers were observed only in Acinetobacter isolates. The most frequent genotype observed was blaOXA-23+/blaOXA-51+ (45,5%), followed by blaOXA-51+/blaOXA-143+ (41%). The genes blaOXA-24 and blaOXA-58 were not detected. High mortality rate (> 70%) between the pacients was observed. In a prospective study, advanced age, predominance of individuals hospitalized in ICU, use of invasive medical devices, such as venous catheter, previous treatment with fluoroquinolones or β-lactams in combination with β-lactamase inhibitor and prolonged stay in hospital were predisposing factors for infection by these microorganisms. Colistin has shown activity, in vitro, against all assessed bacterial samples. Tigecycline was also effective for strains of A. baumannii. Carbapenem-resistant P. aeruginosa was not able to produce hemolysin. These strains were less tolerant to oxidative stress and some biocides, but they showed increased ability of biofilm formation in relation to susceptible isolates. The major mechanisms of carbapenems resistance present in A. baumannii strains was oxacilinases synthesis (OXA-23, OXA-51 and OXA-143), oprD absence and efflux pump presence (AdeABC). In P. aeruginosa was found genes encoding efflux pumps (MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY-OprM) and blaSPM-1; besides oprD absence. These results confirm the difficulty of therapeutic management of patients with infections associated with multidrug resistant microorganisms, with direct impact on mortality and epidemiological control of multidrug-resistant strains in health centers.
Clímaco, Eduardo Carneiro. "Análise molecular de mecanismos determinantes de resistência a antibióticos em Pseudomonas aeruginosa e Acinetobacter ssp." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-20102011-151623/.
Повний текст джерелаThe non-fermenting pathogenic bacteria Pseudomonas aeruginosa and Acinetobacter spp. are important causes of nosocomial infections. Theses species are often associated with a multidrug resistance (MDR) phenotype, due to intrinsic and acquired resistance genes. Some determinants of resistance, such as integrons, carbapenemases, overexpression of efflux systems and porins loss may be associated with the MDR phenotype. The aim of this study was to evaluate the association of non-MDR and MDR phenotypes in P. aeruginosa and Acinetobacter spp. to the presence of integrons and carbapenemases encoding genes, the overexpression of mexY, mexB, mexD and adeB genes and loss of the outer membrane protein, OprD. These resistance determinants were evaluated in 147 P. aeruginosa and 57 Acinetobacter spp., isolated from in-patients of University Hospital of UFJF. Isolates with different PFGE and non-susceptibility profiles were grouped according to MDR or non-MDR phenotypes. PCR and real-time RT-PCR were used to investigate the presence of class 1, 2 and 3 integrons and carbapenemase encoding genes and the expression of mexY, mexB, mexD and adeB efflux pumps and OprD porin, respectively. Class 1 integrons were one of the most common genetic elements present in MDR P. aeruginosa (44,2%), but the phenotype could not be attributed to these elements, since they showed empty (43/65) or only aminoglycoside gene cassettes (19/65). Class 2 integrons were the most common genetic elements in MDR Acinetobacter spp., and this association was statistically significant. SPM encoding gene was the only carbapenemase gene found in P. aeruginosa and, predominantly, in the PFGE cluster A. Expression of MexXY-OprM determined by real-time RT-PCR was the highest variable between MDR and non-MDR P. aeruginosa isolates (almost 10-fold). Reduction of 66.4% in OprD expression was observed in MDR P. aeruginosa, in comparison with non-MDR ones. It is concluded that the most important genetic determinants in the MDR phenotype of P. aeruginosa were SPM-1 production, followed by MexXY-OprM over expression and diminished production of OprD, while class 2 integrons was the most important genetic determinant of MDR phenotype in A. baumannii.
Nascimento, Laura de Oliveira. "Porinas e suas ações imunomoduladoras dependentes de TLR2." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/9/9135/tde-21032013-125043/.
Повний текст джерелаMicroorganisms can invade the host through many routes, specially the respiratory tract. The respiratory mucosa is responsible for recognition, inhibition, proliferation and entry blockade of microorganisms, besides incitation of immunological memory to prevent further infections. Some microorganisms, such as Neisseria meningitidis and Neisseria lactamica, can modulate the mucosa immune response directly or through stimulation of respiratory epithelial cells. The present work proposed the evaluation of porin B proteins, derived from these microorganisms, as modulators of IL-8 production on respiratory epithelial cell strains BEAS-2B and Detroit 562. TLR2 receptor dependency for the modulation was also evaluated. Both porins bounded to TLR2 and through this receptor were able to stimulate IL-8 production, whereas this profile was correlated with TLR2 expression. Lactamica porin (Nlac PorB) induced less IL-8 and TLR2 expression, also for a shorter period of time. The effect caused by Nlac PorB was attributed to TLR2 down regulated expression, since its binding affinity to the receptor is greater than meningococcal porin (Nmen PorB). Porins were therefore able to immune modulate mucosal cells, fact that allied with their parenteral adjuvant activity incited evaluation of porins as potential mucosal adjuvants. The model chosen for the evaluation was intranasal immunization of mice, using as the antigen a low immunogenic lipopolysaccharide extracted from attenuated Franciscella tularensis (Ft-LPS). The evaluation was based on IgG and IgM serum titers. After the immunization scheme, Nmen PorB induced higher IgG and IgM titers than Nlac PorB. Although Nmen PorB was more efficient, it comes from a pathogen. To overcome the risk of its production, it was replaced by recombinant porin (rPorB) produced by Escherichia coli. rPorB was evaluated by the same model and compared with well known experimental adjuvants (rCTB, QS-21 e ODN 1826). rPoB had the highest IgM and IgG titers among all adjuvants tested, specially two months after vaccination. The same adjuvant was also combined with a viral antigen to characterize its application wideness and biological function of incited antibodies. Results obtained with rabies antigen by intranasal route confirmed the mucosal adjuvant properties of rPorB, increasing IgG titers induced by the antigen. These antibodies were also capable of virus neutralization, as demonstrated in RFFIT assays. rPoB didn´t raise neutralizing antibody titers by subcutaneous route, but increased IgG titers. Cellular immunity was undetectable in spleen lymphocytes with the screening method used, regardless of adjuvant addition. In conclusion, porins were characterized as revelant for immunomodulation of the respiratory mucosal cells, caused by infection with meningococcus. The immunomodulation was also revelant for increase of humoral reponse to different antigens and by different routes, pointing out recombinant porin B as an efficient and versatile immunological adjuvant.
Neves, Patricia Regina. "Alterações da permeabilidade e expressão de bombas de efluxo em isolados clínicos de Pseudomonas aeruginosa resistente ao imipenem." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-09022012-162542/.
Повний текст джерелаIntroduction: Clinical isolates of Pseudomonas aeruginosa are associated with high mortality rates. Resistance to imipenem is a global concern, since it is a drug of choice for the treatment of infections produced by multidrug-resistant Gram-negative bacteria. Thus, research on resistance mechanisms is crucial to carry out an effective program for infection control and epidemiology of imipenem-resistant strains. Objective: to characterize the major mechanisms of imipenem resistance in 76 clinical isolates of P. aeruginosa recovered from clinical samples collected, from 2004 to 2007, in four hospitals in the State of São Paulo, Brazil. Material and methods: Isolates were screened for: i) resistance profile to antibacterial agents, determining the MIC of imipenem; ii) the detection of metallo-beta-lactamase (MBL) by phenotypic and genotypic methods, iii) MBL detection by using a double-disk diffusion test (D-test), determining the sensitivity and specificity of the assay; iv) the presence of genes encoding 16S rRNA methylases and their association with aminoglycoside-resistant phenotypes, v) changes in the bacterial permeability due to porin (OprD) loss; vi) the presence or absence of the oprD gene by using PCR; vii) phenotypic expression of efflux pumps by determining the MIC of quinolones, cephalosporins and carbapenems in the presence/absence of specific inhibitors, performing a comparative analysis with a combined-disk method, viii) genes encoding efflux pumps proteins (mexC and mexX) by PCR; ix) MexAB-OprM and MexEF efflux pumps expression; x) clonal relatedness, by ERIC-PCR genotyping, regarding the predominance of major resistance genotypes. Results: Among imipenem-resistant P. aeruginosa strains (n=76, MIC50 e MIC90 = 32 µg/mL e > 512 µg/mL, respectively) 82% showed a multidrug-resistant phenotype. The main mechanism of imipenem resistance was the MBL production detected in 74% strains, of which 62% harbored the blaSPM-1 gene, and 12% harbored the blaVIM-like gene. The D-test identified MBL production in 61% strains. In this regard, CAZ/MAA was the most sensitive combination for MBL detection associated to SPM-1 enzyme (89%), exhibiting 86% specificity. The presence of the rmtD 16S rRNA methylase gene was confirmed in 66% aminoglycoside-resistant strains. Moreover, presence of both rmtD and blaSPM-1 genes was identified in 61% strains. Loss of OprD porins was observed in 71% strains. In this regard, 66% MBL positive strains and 85% MBL negative strains showed OprD loss. Thus, MBL production and OprD loss contributed to imipenem resistance in P. aeruginosa. Most likely, in 13% (10/76) strains the porin loss was associated to insertion sequences (SI) inserted upstream of the oprD gene. On the other hand, in 11% (9/76) strains the absence of a PCR product targeting the 736/1394 region of the oprD gene, suggested the presence of polymorphisms. The mexA gene was identified in 92% strains, whereas the mexE gene was identified in 82% strains. Results obtained from efflux pump screening by using a combined-disk assay and MIC determination in the presence of reserpine, CCCP e PABN (using levofloxacin, meropenem, aztreonam or imipenem) was not correlated with results obtained from MexAB-OprM and MexEF-OprN overexpression analysis by RT-PCR. In this regard, both combined-disk and MIC assay showed lack of specificity and sensitivity in comparison to RT-PCR. Overexpression of mexA and mexE genes was confirmed in 35% (7/20) MBL-negative and 11% (6/56) MBL-positive strains, respectively, being 7% (4/56) MBL-positive strains overexpressed both genes. The overexpression of MexAB-OprM and MexEF-OprN efflux pumps, as only mechanism of resistance to meropenem and imipenem was observed in 10% (2/20) MBL-negative strains. ERICPCR typing revealed the presence of 24 clusters among 76 imipenem-resistant P. aeruginosa strains (≥ 90% similarity). Conclusion: The convergence of multiple mechanisms of resistance in Pseudomonas aeruginosa seems to be a favorable event for the selection of multiresistant clones endemic in the southeastern region of Brazil.
Guzmán, Santibáñez Diego Ignacio. "Influencia del vaporizado sobre la resistencia a la pudrición de madera de renoval de roble (Nothofagus obliqua (Mirb.) Oerst)." Tesis, Universidad de Chile, 2017. http://repositorio.uchile.cl/handle/2250/151386.
Повний текст джерелаNothofagus obliqua (Mirb.) Oerst (roble o pellín), es una especie que se distribuye desde la Región de Valparaíso hasta la Región de Los Lagos; también habita en Argentina. Esta especie se caracteriza por la alta durabilidad natural que presenta su madera en individuos adultos. Sin embargo, para la madera proveniente de renovales, con diferentes características de color y densidad, no existen antecedentes sobre su comportamiento frente al ataque de hongos de pudrición u otros agentes de biodeterioro. Actualmente, la madera de renovales de roble es sometida a un tratamiento de “vaporizado”, el que tiene como objetivo igualar el color rosado a rojizo del duramen de la madera de renoval, con el rojo oscuro del duramen de roble pellín, mucho más apreciado en el mercado. Con el objetivo de proporcionar antecedentes sobre la durabilidad natural de la madera de renoval de roble y de la influencia del tratamiento de cambio de color (vaporizado), se evaluó la resistencia de estas maderas frente a la acción de hongos de pudrición mediante la pérdida de peso, solubilidad en soda y el % de lignina. Para realizar el estudio se utilizó madera de renoval de roble, empleándose 40 probetas de albura y 40 de duramen. Se aplicó el proceso de vaporizado a 20 probetas de cada grupo, de manera de compararlas con el comportamiento de la madera sin vaporizar frente al ataque del hongo de pudrición blanca Polystictus versicolor y de pudrición café Poria monticola, utilizando además, 20 probetas de roble adulto como testigo. La mitad de las probetas de cada conjunto fue evaluada a los 2 meses (T1) y el resto a los 4 meses (T2) desde el inicio del ataque fúngico. Los resultados indicaron que el vaporizado no tuvo influencia sobre la resistencia de la madera de renoval frente al ataque de P. versicolor, que generó el %PP más alto para todos los tipos de madera a los 2 meses de acción fúngica. Así mismo, el % de solubilidad en soda indicó que el hongo de pudricion blanca provocó el mayor biodeterioro en comparación con el hongo de pudricion café. Para el caso de P. monticola, se observó que el vaporizado tampoco tuvo efecto sobre la resistencia al ataque de pudrición, generándose el mayor biodeterioro a los 4 meses (T2) que a los 2 meses (T1). Esto señaló finalmente, que la madera de renoval de roble no es una madera durable, considerando su resistencia frente al ataque de hongos de pudrición.
Nothofagus obliqua (Mirb.) Oerst (roble o pellín), is a specie allocated from Valparaiso Region to Los Lagos Region, Chile, also it can be found in Argentina. This kind is characterised by its high natural durability of its wood in adults members. Nevertheless, there are not history, for wood from secondary forests, with different characteristics of color and density, about its behaviour against the fungal attack or other biodeterioration agents. Nowadays, the roble wood from secondary forests is subjected to a “steaming” treatment, which aims to equalize pink to reddish heartwood color of the wood from secondary forests, with the dark red of the roble pellin heartwood, much more appreciated in the market. In order to provide history about the natural durability of the roble secondary forest wood, and of the influence of the color change treatment (steaming), the resistance of these woods against the action of fungal through weightloss, soda solubility and the lignin percentage was evaluated. Roble wood (renoval) was used to carry out the research, using 40 test tubes for sapwood and 40 test tubes for heartwood. Steaming process was tested in 20 test tubes of each group to do the comparison with those samples that were not under the process but under the white rot fungi attack Polystictus versicolor and the brown fungi Poria monticola. Also 20 test tubes of old roble were used as reference sample. Half of the samples of each group was checked at two months (T1) and the remaining samples at four months (T2) since the attacks of fungi started. The results shown that the steaming did not affect the wood resistance against the attack of P. versicolor produced the highest %PP for all the samples at 2 months of the test. Moreover, the %S indicated that the white fungi produced a bigger biodeterioration than the brown one. In the case of P. Monticola, it was observed that the steaming had no effect neither about the resistance of fungal attack, generating the greatest deterioration at 4 months (T2) than 2 months (T1). This finally pointed that the roble secondary forest wood is not a durable one, considering its resistance against the fungal attack
Книги з теми "Porirua"
1940-, Murray Bruce, Wood David R, and Tawa Historical Society, eds. Best of Tawa: Porirua, and they who settled it : first published in the Canterbury times, 11 March 1914 to 1 July 1914. Wellington, N.Z: Tawa Historical Society, 2007.
Знайти повний текст джерелаPowys, Theodore Francis. Porius. London: Overlook Duckworth, 2007.
Знайти повний текст джерелаRimon, D. Poriyut ha-ḳarḳaʻ. Ramat ha-Sharon: D. Rimon, 1991.
Знайти повний текст джерелаPowys, Theodore Francis. Porius: [a novel]. Edited by Krissdottir Morine and Bond Judith. New York: Overlook Duckworth, 2007.
Знайти повний текст джерелаProgramme, Rainbow Reading, and Nelson Education Centre, eds. Biking the Porika. Nelson, N.Z: Rainbow Reading Programme, 1995.
Знайти повний текст джерелаLivadas, Vias. Amtistrofi poria. Cyprus: Livadas, 1986.
Знайти повний текст джерелаChristodoulou, Miltiades. I poria mias epohis. Athens: Floros, 1987.
Знайти повний текст джерелаO Rakičevom pesimizmu i srpskom samoubilačkom porivu. Beograd: Narodna Knjiga, Alfa, 2000.
Знайти повний текст джерелаKeith, W. J. John Cowper Powys's Porius: A reader's companion. [Toronto: s.n.], 2005.
Знайти повний текст джерелаPowys, Theodore Francis. Porius: A romance of the Dark Ages. Edited by Albrecht Wilbur T. [Hamilton, N.Y.]: Colgate University Press, 1994.
Знайти повний текст джерелаЧастини книг з теми "Porirua"
Wagner, Hildebert, Rudolf Bauer, Dieter Melchart, Pei-Gen Xiao, and Anton Staudinger. "Poria — Fuling." In Chromatographic Fingerprint Analysis of Herbal Medicines, 923–34. Vienna: Springer Vienna, 2011. http://dx.doi.org/10.1007/978-3-7091-0763-8_75.
Повний текст джерелаBährle-Rapp, Marina. "Poria Cocos Extract." In Springer Lexikon Kosmetik und Körperpflege, 442. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_8240.
Повний текст джерелаHu, C., R. Nögel, J. Hummelsberger, and U. Engelhardt. "Monographie 44: Poria (Fuling 茯苓)." In Paozhi: Die Aufbereitung chinesischer Arzneimittel, 379–84. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-55846-1_49.
Повний текст джерелаGou, Xiao-jun, Gang He, and Xiao-qiang Guo. "Poria cocos (Schw.) Wolf 茯苓 (Fuling, Indian Bread)." In Dietary Chinese Herbs, 781–88. Vienna: Springer Vienna, 2015. http://dx.doi.org/10.1007/978-3-211-99448-1_87.
Повний текст джерела"SERMO, IN PATRES, QUI TEM PORIBUS SUIS CONSUM MATI SUNT." In Works Found in Greek Attributed to Ephrem, edited by Gerhard Vossius, 766–69. Piscataway, NJ, USA: Gorgias Press, 2010. http://dx.doi.org/10.31826/9781463224615-035.
Повний текст джерела"Reconceptualising dark tourism AvITAL BIRAN AND YANIv PORIA." In Contemporary Tourist Experience, 75–86. Routledge, 2014. http://dx.doi.org/10.4324/9780203139110-11.
Повний текст джерелаYang, H., Y. F. Yang, L. Q. Duan, Q. J. Ling, and Z. Huang*. "Poria cocos polysaccharide decorated selenium nanoparticles attenuate colitis by suppressing hyper inflammation." In Selenium Research for Environment and Human Health: Perspectives, Technologies and Advancements, 221–22. CRC Press, 2019. http://dx.doi.org/10.1201/9780429423482-102.
Повний текст джерелаLi, Xiulian, Lina Ma, and Lijuan Zhang. "Molecular basis for Poria cocos mushroom polysaccharide used as an antitumor drug in China." In Progress in Molecular Biology and Translational Science, 263–96. Elsevier, 2019. http://dx.doi.org/10.1016/bs.pmbts.2019.02.011.
Повний текст джерелаKuzuoglu, Sina, and Stella Kladou. "Challenges to sustainability in prospective world heritage sites." In Sustainable and Collaborative Tourism in a Digital World. Goodfellow Publishers, 2021. http://dx.doi.org/10.23912/9781911635765-4846.
Повний текст джерелаТези доповідей конференцій з теми "Porirua"
Barrantes, Francisco, Andrew McMenamin, and Roger Tang. "Hutt River pipeline bridge spanning across the Wellington fault." In IABSE Congress, Christchurch 2021: Resilient technologies for sustainable infrastructure. Zurich, Switzerland: International Association for Bridge and Structural Engineering (IABSE), 2021. http://dx.doi.org/10.2749/christchurch.2021.0513.
Повний текст джерелаSal, Neda, and Fiona Beals. "Foreword." In 2021 ITP Research Symposium. Unitec ePress, 2022. http://dx.doi.org/10.34074/proc.2205001.
Повний текст джерелаWang, Wenjie, Zhian Zheng, Lei Gao, Xiongchu Zhang, Zimei Zhang, and Rongyan Wang. "Design of intelligent horizontal Poria cocos peeling machine." In 2021 ASABE Annual International Virtual Meeting, July 12-16, 2021. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2021. http://dx.doi.org/10.13031/aim.202100299.
Повний текст джерелаZhang, Xianyi, Runyu Wang, Rongze Jin, Shuxin Ji, and Mengzhen Tian. "Application of poria cocos in the treatment of heart disease." In 4TH INTERNATIONAL CONFERENCE ON FRONTIERS OF BIOLOGICAL SCIENCES AND ENGINEERING (FBSE 2021). AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0094311.
Повний текст джерелаSi, Shan, Nie Peng, Jiang Li, Yuwen Yan, and Xiaojun Yan. "Bioinformatics Analysis on Molecular Mechanism of Poria Cocos in Treatment of Jaundice." In 2016 International Conference on Computer Engineering, Information Science & Application Technology (ICCIA 2016). Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/iccia-16.2016.4.
Повний текст джерелаZheng, Zhian, Ping Zhang, Jiao Wang, Yuxin Zhang, and Zusongying Zhao. "Evaluation of different pretreatments for <i>Poria cocos</i>." In 2018 Detroit, Michigan July 29 - August 1, 2018. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2018. http://dx.doi.org/10.13031/aim.201800841.
Повний текст джерелаGuo, Jing, Peng Zeng, Meng Fang, and Han Zhao. "Network Pharmacological Analysis and Preliminary Validation of Mechanisms of Poria cocos (Schw.) Wolf Against Stroke." In 2021 International Conference on Public Health and Data Science (ICPHDS). IEEE, 2021. http://dx.doi.org/10.1109/icphds53608.2021.00058.
Повний текст джерелаZheng, Zhian, Ping Zhang, Yuxin Zhang, and Zusongying Zhao. "Effects of different pretreatments on quality attributes of <i>Poria cocos</i>." In 2018 Detroit, Michigan July 29 - August 1, 2018. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2018. http://dx.doi.org/10.13031/aim.201800839.
Повний текст джерелаWang, Wenjie, Zhian Zheng, Shufeng Chen, Shanyu Wang, and Andi Chen. "Effect of different temperature and humidity stress combined with steaming on the quality of Poria cocos peel." In 2021 ASABE Annual International Virtual Meeting, July 12-16, 2021. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2021. http://dx.doi.org/10.13031/aim.202100297.
Повний текст джерелаZheng, Zhi�an, Zusongying Zhao, Ping Zhang, Qingwu Jiang, Bo Lin, and Pengtao Li. "<i>Poria cocos Soil-transfer Cultivation Experiment and Its Impact on Yield</i>." In 2017 Spokane, Washington July 16 - July 19, 2017. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2017. http://dx.doi.org/10.13031/aim.201700936.
Повний текст джерелаЗвіти організацій з теми "Porirua"
Wang, Qin, Xinya Peng, and Congchao Jia. Efficacy and Pharmacological Mechanism of Poria cocos-based Formulas Combined with Chemotherapy for Ovarian Cancer: A Integrated Systems Pharmacology Study. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, August 2021. http://dx.doi.org/10.37766/inplasy2021.8.0060.
Повний текст джерела