Дисертації з теми "Platelet rich plasma scaffold"
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Talamas, Ugalde Lucet Vanessa. "Effects of platelet rich plasma on marrow stromal cells differentiation seeded on three dimensional scaffolds." To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2008. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.
Повний текст джерелаFontenot, Robin L. "Tube centrifugation for processing platelet-rich plasma in the horse." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/76971.
Повний текст джерелаMaster of Science
Thor, Andreas. "On platelet-rich plasma in reconstructive dental implant surgery /." Göteborg : Departments of Biomaterials and Oral & Maxillofacial Surgery, the Sahlgrenska Academy at Göteborg University and the Department of Surgical Sciences, Oral & Maxillofacial Surgery, Uppsala University Hospital, 2006. http://hdl.handle.net/2077/745.
Повний текст джерелаO'Shea, Caitlin Mary. "Comparison of platelet counting technologies in equine platelet concentrates." Thesis, Virginia Tech, 2014. http://hdl.handle.net/10919/56837.
Повний текст джерелаMaster of Science
Baboldashti, Nasim Zargar. "Platelet rich plasma and mechanical loading in regenerative tendon repair." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.556118.
Повний текст джерелаCundell, Jill Hannah. "Evaluation of the characteristics of leucocyte-rich platelet-rich plasma from participants with diabetes." Thesis, Ulster University, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.692821.
Повний текст джерелаHerrmann, Sebastian [Verfasser]. "Quantifizierung von Wachstumsfaktoren in Bohrmehl, Beckenkamm-Spongiosa, Platelet Poor und Platelet Rich Plasma / Sebastian Herrmann." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1076270794/34.
Повний текст джерелаWang, Xiao. "Tendon tissue engineering using skills and platelet rich plasma : A multidisciplinary strategy." Thesis, University of Oxford, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.533891.
Повний текст джерелаYavorskyi, A. V. "Clinical efficiency of platelet rich plasma use in patients with odontogenous jaw." Thesis, БДМУ, 2020. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/17837.
Повний текст джерелаAlsousou, Joseph. "Platelet rich plasma in regenerative tendon repair mechanobiological interactions and clinical application." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:2692c00e-0c0d-4096-8daa-c00ad47fd03d.
Повний текст джерелаNugent, Ryan P. "Therapeutic efficacy of platelet-rich plasma injections in treating high hamstring tendinopathy." Thesis, Boston University, 2013. https://hdl.handle.net/2144/12175.
Повний текст джерелаHamstring muscle and tendon injuries are commonly seen in sports medicine clinics, especially in track and field athletes. However, a less common subset of these injuries has recently begun to gain more attention among researchers and clinicians and is referred to as high hamstring tendinopathy. Patients suffering from this condition typically report deep buttock pain brought on by running, or in severe cases even by prolonged sitting, such as in driving a car for long periods of time. Diagnosis of this condition requires a specific patient history, positive signs on physical exams, as well as positive findings on imaging studies, primarily MRI and ultrasound. Treatment for high hamstring tendinopathy typically starts with conservative measures for pain management including ice, electrical stimulation, and pulsed ultrasound. As soon as patients are able, a physical therapy protocol is prescribed involving stretching, along with strengthening, progressing to eccentric exercises. In cases where symptoms are persistent, more aggressive treatments can be followed, such as corticosteroid injections, extracorporeal shockwave therapy, and surgery in more severe cases. However, because these therapies are often ineffective, expensive, and potentially carry high risks, physicians have begun to turn to alternative forms of therapy. One such treatment gaining recent popularity is the use of platelet-rich plasma injections (PRP). The widely held belief is that PRP provides a higher than average concentration of growth factors than is normally contained in platelets and that these stimulate the wound healing cascade and help in tissue repair and regeneration. PRP is prepared using autologous whole blood from patients by two rounds of centrifugation, separating and concentrating the platelets from other components such as red blood cells and leukocytes. [TRUNCATED]
Dohan, David. "Platelet Rich Fibrin (PRF) : modèle théorique et études préliminaires." Paris 5, 2005. http://www.theses.fr/2005PA05M004.
Повний текст джерелаPRF (Platelet Rich Fibrin) is a surgical additive coming from the autologous fibrin adhesives and first generation platelet concentrates (PRP) technologies. The objective of this work is to make a historical and technological synthesis of this family of fibrin related surgical additives, to create a first theoretical model of the PRF molecular architecture and finally, with clinical observations and histological analysis, to evaluate the healing potential of the PRF in soft tissue wounds and during bone graft maturation
Laurens, Ilze. "Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/40717.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2013
gm2014
Pharmacology
unrestricted
Plümacher, Pascal Simon [Verfasser]. "Untersuchung zur chondrogenen Potenz von platelet-rich plasma versus Hämarthros / Pascal Simon Plümacher." Kiel : Universitätsbibliothek Kiel, 2017. http://d-nb.info/1137509821/34.
Повний текст джерелаRicciardi, Giulia <1981>. "Use of platelet rich plasma in tendons' and ligaments' injuries in sport horses." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/4003/.
Повний текст джерелаPedvis, Lloyd Gary. "Differential activation and inhibition of human platelet shape change, micro- and macroaggregation, in whole blood and platelet-rich plasma." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61878.
Повний текст джерелаForsyth, Sara. "The effect of exercise on the concentration of platelets in a platelet rich plasma preparation." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/40420.
Повний текст джерелаHaas, Holger [Verfasser]. "Der Einfluss von platelet-rich Plasma auf die immunologisch induzierte Kniegelenkarthritis im Grosstiermodell / Holger Haas." Kiel : Universitätsbibliothek Kiel, 2014. http://d-nb.info/106253610X/34.
Повний текст джерелаMalboubi, Saeid. "In vitro actions of platelet rich plasma and resolvin E1 on osteoblast and osteoclast activity." Thesis, Boston University, 2009. https://hdl.handle.net/2144/35619.
Повний текст джерелаIncludes bibliographic references: leaves 52-59.
Platelet-rich plasma (PRP) is a concentrated gel of platelets that contains several growth factors. Growth factors have been recognized as the part of PRP that play role in regeneration of the bone. It is not clear how these growth factors in PRP affect the bone regeneration. Resolvin El (RvEl; 5S,12R,18R-trihydroxyeicosapentaenoic acid) is an pro-resolving lipid mediator derived from omega-3 fatty acid eicosapentaenoic acid and shown to have potent effects on the resolution of inflammation. The purpose of this study was to analyze the action of PRP and RVEl on the proliferation and behavior of osteoblasts and osteoclasts in vitro. PRP was prepared from 14 healthy donors. Osteoblast cultures were from a cell line (Saos2) of osteosarcoma cells. Osteoclasts were differentiated from primary human peripheral blood monocytes. Osteoclastic morphology was studied and activity was analyzed via resorption on dentin discs using SEM. PRP and RVE 1 were added at different doses and time-points. Osteoblast function was analyzed by osteocalcin expression and release. Osteoclast activity was assessed by resorption and cathepsin K expression. PRP and RvEl comparably increased the osteoblastic activity and suppressed the osteoclast differentiation and function. These results suggest that multiple tools are available to reverse the inflammation and restore the lost bone architecture as a result of periodontal disease.
Upchurch, David A. "Administration of adipose-derived stromal vascular fraction and platelet rich plasma in dogs with coxofemoral osteoarthritis." Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/19769.
Повний текст джерелаDepartment of Clinical Sciences
Walter Renberg
Objective: To evaluate the safety and effect of a single simultaneous intra-articular and intravenous injection of autologous adipose-derived stromal vascular fraction (SVF) and platelet rich plasma (PRP) on coxofemoral osteoarthritis (OA) in dogs. Methods: This was a randomized, double-blind, placebo-controlled prospective pilot trial of simultaneous intra-articular and intravenous SVF and PRP for coxofemoral OA. Dogs with coxofemoral OA causing signs of lameness or discomfort were evaluated by orthopedic exam, visual lameness score, Canine Brief Pain Inventory (CBPI), goniometry, visual analogue scale (VAS), and pressure-sensitive walkway (PSW) at week 0 (baseline), and at 4, 8, 12 and 24 weeks after injection. Joint radiographs were scored at 0 and 24 weeks. Results: Twenty two client-owned dogs with naturally occurring OA of the coxofemoral joints were enrolled (12 placebo-control, 10 SVF-treated). CBPI pain severity scores were lower in the treatment group at 24 weeks compared to the placebo group (p=0.042). The VAS score for the treatment group was significantly greater at 0 weeks than at 4, 8, or 24 weeks (p<0.05). When dogs with low quartile baseline PVF (25th percentile) were compared, the treatment group had statistically higher PVF at all post-injection time points when compared to the placebo group. After SVF injection, fewer dogs in the treated group were lame compared to the control group. Clinical Significance: This study is the first to utilize objective data from PSW as an outcome measure for dogs treated with SVF and PRP for coxofemoral OA. No adverse events were noted. Improvements in some measured parameters in the treated dogs compared to those in the placebo group.
Perko, John C. "Applying Mesenchymal Stromal Cells and Platelet-Rich Plasma on a Collagen Matrix to Improve Fascial Repair." Youngstown State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1342115316.
Повний текст джерелаDiehl, Michael W. "Prevention of Incisional Hernias Using Mesenchymal Stromal Cells and Platelet-Rich Plasma treated Collagen Matrix Tape." Youngstown State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1402572449.
Повний текст джерелаMinteer, Tanya E. "Detection of Collagen in Rat Abdominal Wound Healing: Contributions of Mesenchymal Stromal Cells and Platelet-Rich Plasma." Youngstown State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348848443.
Повний текст джерелаZahn, Jessica [Verfasser], Markus [Akademischer Betreuer] Loibl, and Lukas [Akademischer Betreuer] Prantl. "Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System / Jessica Zahn ; Markus Loibl, Lukas Prantl." Regensburg : Universitätsbibliothek Regensburg, 2020. http://d-nb.info/1204635900/34.
Повний текст джерелаYamada, Ana Lúcia Miluzzi [UNESP]. "Efeito do implante autólogo de plasma rico em plaquetas (PRP) e células tronco mesenquimais na reparação de lesões condrais articulares induzidas experimentalmente em equinos." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/88981.
Повний текст джерелаFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Lesões na cartilagem articular representam um desafio para a Medicina Veterinária devido ao limitado potencial intrínseco de reparação. As células tronco mesenquimais (CTM) e o plasma rico em plaquetas (PRP) vêm sendo utilizados na reparação condral pela capacidade de imunomodulação, quimiotaxia, por diminuir a degradação da cartilagem articular e exercer efeito anti-inflamatório. Esse trabalho estudou a eficácia das CTM e do PRP no tratamento de lesões condrais articulares, experimentalmente induzidas em equinos, apresentando os benefícios e os principais obstáculos encontrados na aplicação clínica e cirúrgica de equinos. Para isso, foi induzida uma lesão condral, na tróclea medial femoral dos dois membros pélvicos de oito animais. Após 30 dias da indução dessa lesão os animais, divididos em quatro grupos, receberam os tratamentos propostos: com CTM (Grupo 1); PRP (Grupo 2); CTM e PRP (Grupo 3) e um controle sem tratamento (Grupo 4). As avaliações dos parâmetros clínico-laboratoriais foram realizadas antes da indução da lesão (T-0), quinzenalmente até 120 dias e aos 150 dias (T-150). Avaliações macroscópicas, histopatológicas e histoquímicas foram realizadas em T-0 e T- 150. Pôde-se observar discreta melhora clínico-laboratorial dos grupos tratados quando comparados ao grupo controle. Os exames macroscópicos, histopatológicos e histoquímicos revelaram um melhor tecido de reparação nos grupos tratados, principalmente nos grupos 2 e 3. Sendo assim, o implante autólogo de CTM e do PRP apresentou efeitos benéficos no tratamento de lesões condrais, experimentalmente induzidas em equinos
Articular cartilage defects represent a challenge for Veterinary Medicine due to the limited intrinsic potential of repair. Mesenchymal Stem Cells (MSC) and Platelet-Rich Plasma (PRP) have been used in cartilage repair to promote immunomodulation, chemotaxis, to decrease the degradation of articular cartilage and to take antiinflammatory effect. This work aims to study the use of intralesional MSC and the PRP in the treatment of articular cartilage defects induced experimentally in horses, noting the benefits and challenges encountered in implementing clinical-surgical in horses. For this purpose, both patellofemoral joints of eight animals were approached by arthroscopic surgery to perform a cartilage defect, on the medial femoral trochlea. After 30 days of induction the animals, who were divided into four groups, received treatment with: MSC (Group 1), PRP (Group 2), MSC and PRP (Group 3) and a control without treatment (Group 4). The clinical and laboratory assessments were performed every 15 days in 120 days, with last analysis in day 150. The macroscopic and morphologic analyses were performed at 0 and 150 day. There were clinical and laboratory improvement in the treated groups compared with control group. The macroscopic and morphological analysis showed a better tissue repair in the treated groups, mainly in groups 2 and 3. Thus, the implantation of autologous MSC and PRP showed beneficial effects in the treatment of chondral lesions, experimentally induced in horses
Deliberador, Tatiana Miranda. "Associação do plasma rico em plaquetas, novo vidro bioativo e sulfato de cálcio no tratamento de defeitos de furca classe II : estudo histológico e histométrico em cães /." Araçatuba :, 2007. http://hdl.handle.net/11449/104714.
Повний текст джерелаBanca: Ronaldo Célio Mariano
Banca: Valdir Gouveia Garcia
Banca: Mário Taba Júnior
Banca: Álvaro Francisco Bosco
Resumo: O objetivo do presente estudo foi avaliar, histologicamente, o processo de cicatrização de defeitos de furca Classe II, criados cirurgicamente e tratados com a associação do Plasma Rico em Plaquetas (PRP), partículas esféricas de Novo Vidro Bioativo (NVB) e Sulfato de Cálcio (SC). Os segundos e quartos prémolares mandibulares de 8 cães foram usados neste estudo. Defeitos de furca Classe II (5 mm de altura x 2 mm de profundidade) foram criados cirurgicamente e imediatamente tratados. Usando o modelo de boca dividida, os dentes foram aleatoriamente divididos em 2 grupos: Grupo C (controle): o defeito foi preenchido somente com coágulo sanguíneo e Grupo T (teste): o defeito foi preenchido com a associação de PRP, NVB e SC. Os retalhos foram reposicionados para cobrir totalmente os defeitos. A eutanásia dos animais foi realizada aos 90 dias pósoperatórios. Foram obtidos cortes histológicos seriados mésio-distais, corados com Hematoxilina e Eosina. Foram realizadas análises histométrica, usando um software analisador de imagens, e histológica. Foram avaliadas medidas lineares e de área da cicatrização periodontal, calculadas como porcentagem do defeito original. Os dados percentuais foram transformados em arcoseno para análise estatística (análise de variância, p < 0,05). A regeneração óssea e de tecido conjuntivo nos defeitos de furca foi incompleta na maioria dos espécimes. O Grupo C apresentou quantidade de neoformação óssea e regeneração periodontal significativamente maior que o Grupo T. Quantidade considerável de partículas remanescentes de NVB foi observada na maioria dos espécimes do Grupo T. Dentro dos limites do presente estudo, pode-se concluir que a associação do PRP, NVB e SC não foi mais efetiva que o debridamento cirúrgico somente no tratamento de defeitos de furca Classe II.
Abstract: The purpose of this study was to histologically evaluate the healing of surgically created Class II furcation defects treated using a combination of platelet-rich plasma (PRP), new spherical bioactive glass (NBG) particles and calcium sulfate (CS). The second and fourth mandibular premolars of eight mongrel dogs were used in this study. Class II furcation defects (5 mm in height x 2 mm in depth) were surgically created and immediately treated. Using a split-mouth design, teeth were randomly divided into two groups: group C (control) - defect filled with blood clot only; and group T (test) - defect filled with a combination of PRP, NBG and CS. Flaps were repositioned to cover all defects. The animals were euthanized 90 days post-surgery. Mesio-distal serial sections were obtained and stained with either hematoxylin and eosin. Histometric, using image-analysis software, and histologic analyses were performed. Linear and area measurements of periodontal healing were evaluated and calculated as a percentage of the original defect. Percentage data were transformed into arccosine for statistical analysis (analysis of variance; P<0.05). Regeneration of bone and connective tissue in the furcation defects was incomplete in most of the specimens. Group C had significantly more bone formation and periodontal regeneration than group T. A considerable amount of remaining NBG particles was observed in most specimens of group T. Within the limits of this study, it can be concluded that the combination of PRP, NBG and CS was not more effective than the surgical debridement alone in the treatment of Class II furcation defects.
Doutor
Messora, Michel Reis. "Cicatrização de enxertos de osso alógeno fresco congelado (OAFC) associados ou não ao plasma rico em plaquetas (PRP) : estudo histológico e histométrico em mandíbulas de cães /." Araçatuba :, 2009. http://hdl.handle.net/11449/104717.
Повний текст джерелаBanca: Wilson Roberto Poi
Banca: Valdir Gouveia Garcia
Banca: Maria Lucia Rubo de Rezende
Banca: Ronaldo Célio Mariano
Banca: Mario Taba Júnior
Resumo: O propósito deste estudo foi avaliar, histologicamente, a cicatrização de enxertos de osso alógeno fresco congelado (OAFC) associados ou não ao plasma rico em plaquetas (PRP) em defeitos ósseos criados cirurgicamente em mandíbulas de cães. Defeitos ósseos bilaterais, medindo 1,5 cm de largura x 1 cm de altura, foram criados na borda inferior da mandíbula de 10 cães adultos machos. Os defeitos foram divididos em 3 grupos experimentais: C (controle), OAFC, OAFC/PRP. No Grupo C (n=7), os defeitos foram preenchidos apenas com coágulo sangüíneo. No Grupo OAFC (n=7), os defeitos foram preenchidos com enxertos de OAFC. No Grupo OAFC/PRP (n=6), os defeitos foram preenchidos com enxertos de OAFC associados ao PRP. A eutanásia dos animais foi realizada em 12 semanas pós-operatórias. Foram realizadas análises histológica e histométrica. Os dados foram analisados estatisticamente (ANOVA, Tukey, p < 0,05). Nenhum defeito regenerou completamente com tecido ósseo. Os enxertos de OAFC foram bem incorporados. A quantidade média de osso neoformado e os desvios-padrão dos Grupos C, OAFC e OAFC/PRP foram 70,55 8,01%, 71,31 14,36% e 65,57 11,55%, respectivamente. Não foram observadas diferenças estatisticamente significativas entre os grupos (ANOVA, p = 0,642). Os enxertos de OAFC foram biocompatíveis e bem incorporados, mas não proporcionaram maior formação óssea que os defeitos controle em mandíbulas de cães. O uso do PRP não promoveu nenhum benefício adicional à cicatrização desses enxertos em 12 semanas pós-operatórias.
Abstract: The purpose of this study was to histologically analyze the healing of fresh frozen bone allograft (FFBA) with or without platelet-rich plasma (PRP) in bony defects surgically created in mandible of dogs. Bilateral bony defects, measuring 1.5 cm in width vs. 1 cm in height, were created in the inferior border of the mandible of 10 adult male dogs. The defects were divided into three groups: C (control), FFBA and FFBA/PRP. In Group C (n=7), the defect was filled with blood clot only. In Group FFBA (n=7), the defect was filled with FFBA. In Group FFBA/PRP (n=6), the defect was filled with FFBA combined with PRP. All animals were euthanized at 12 weeks post-operative. Histologic and histometric analyses were performed. Data were statistically analyzed (ANOVA, Tukey, p < 0.05). No defect completely regenerated with bone. The FFBA was well incorporated. The mean percentage of newly formed bone and the standard-deviations of Groups C, FFBA and FFBA/PRP were 70.55 8.01%, 71.31 14.36% and 65.57 11.55%, respectively. Statistically significant differences were not found among the groups (ANOVA, p = 0,642). FFBA were biocompatible and well incorporated. However, these grafts did not promote a greater bone formation than the control defects in mandible of dogs. The use of PRP promoted no additional benefit to the healing of these grafts at 12 weeks post-operative.
Doutor
Rafael, Bambo Otilia. "Intestinal anastomosis wound healing after platelet-rich plasma (PRP) application on pigs. Macroscopic, microscopic and breaking strength evaluations." Doctoral thesis, Universitat Autònoma de Barcelona, 2009. http://hdl.handle.net/10803/5748.
Повний текст джерелаDesde hace varios años, los estudios relacionados con la regeneración se han dirigido a investigar cuál es el efecto de los factores de crecimiento sobre los tejidos. Gran parte de los estudios se han realizado in vitro, utilizando factores recombinantes, es decir, factores de crecimiento elaborados en el laboratorio. Pero la desventaja de estas sustancias incluye el alto coste económico para su obtención. Y poco se sabe de sus efectos secundarios.
Actualmente, el estudio del proceso de la cicatrización se centra en la aceleración de este proceso a nivel celular con el uso de plasma rico en plaquetas (PRP). En el presente estudio se han incluido 35 cerdos de raza Large White, sexo indiferente con un peso medio de 55±5 Kg. En cada animal, se realizaron 2 enterectomías, en una de ellas se le aplicó PRP en el lugar de la incisión, mientras que en la otra no, sirviéndonos como control. Para el examen histológico se realizaron 5 grupos de 3 animales cada uno. Los cerdos se eutanasiaron a las 24h, 48h, 72h, 96h y 7 días, respectivamente, tras la cirugía. Para la evaluación de la resistencia de la anastomosis, se emplearon 2 grupos de 10 animales cada uno. El grupo A se eutanasió a los 3 días tras la cirugía, mientras que el grupo B se eutanasió a los 7 días. A nivel histológico en todas las muestras de las 24h y 48h, tanto en las muestras control como PRP, mostraron una creciente inflamación que finalmente, a las 72h y 96h fue substituida por un tejido de granulación inmaduro. La presencia de tejido de granulación fue más abundante en las muestras PRP.
En la determinación de la fuerza de rotura no se obtuvieron diferencias significativas en la resistencia de la anastomosis entre el grupo control y el PRP, para ninguno de los 2 días (3 y 7 días). Sin embargo, aunque los resultados no sean significativos, las mayores fuerzas de rotura obtenidas corresponden a las anastomosis con PRP.
Para la determinación de la activación plaquetar evaluamos los valores del componente plaquetar medio (MPC) en la sangre y PRP obtenidos durante el recuento (ADVIA 120). Los valores del MPC obtenidos en la sangre (21,3±1,58 g/dL) y en el PRP (20,3±1,39 g/dL), no mostraron diferencias significativas. Este hecho sugiere que el protocolo de preparación de PRP no produce una activación significativa de las plaquetas.
Para la determinación de factores de crecimiento usamos el test (ELISA). Los niveles del TGF- β1 han sido determinados en distintas fracciones que se obtenien durante la preparación del PRP: en el PRP (24312 pg/mL) y PRP gel, activado con cloruro cálcico (15504 pg/mL) y en el plasma pobre en plaquetas (PPP) - obtenido después de la segunda centrifugación (3715 pg/mL). En resumen con el protocolo de obtención de PRP utilizado en este estudio se obtuvo una elevada concentración e integridad plaquetar demostrado en los valores del MPC y los niveles de TGF-β1.
Palabras clave: anastomosis intestinal, cerdo, plaquetas, plasma rico en plaquetas, factores de crecimiento.
The principle of performing an intestinal anastomosis consists of apposing the proximal and distal edges of the segments and suturing. Compromised healing may lead to dehiscence and leakage, which is attended by high morbidity and mortality. In small animals the reported incidence of small intestinal dehiscence rates 7-16%, with 74 -80% of those patients dying. Attempts to enhance anastomotic healing have included the use of various surgical techniques and materials: quality of suture threads, use of staples; mechanical protection (endoluminal latex prosthesis, biofragmental ring) and local application of fibrin bioadhesive, fibrin and collagen patches, omental flaps with the most promising results.
One of the most significant advances in the field of modern molecular biology and biochemistry in the last three decades are certainly growth factors and cytokines. Growth factors are biologically active polypeptides influencing growth, differentiation and metabolism of target cells through specific receptors. In the last several years a number of authors investigated the use of various recombinant growth factors (local or systemic) in order to enhance some of the phases of wound healing process.
The disadvantage of these recombinant growth factors is that they are expensive and concerns exist about their safety in human administration. Alternatively, platelet-rich plasma (PRP), which is a volume of autogenous plasma that has a platelet concentration above baseline, is a proven source of growth factors.
Recently, attention has been focused on cellular acceleration of the wound healing process by the use of platelet-rich plasma (PRP). This study determined the effects of PRP application on intestinal healing. Autologous PRP was obtained in 35 Large White breed pigs, males and females, weighting 55±5 Kg, which were submitted to two intestinal anastomosis procedures.
Activated PRP was applied in one of the anastomosis sites, while the other site served as control. After 24 h, 48 h, 72 h, 96 h and 7 days, histology was performed, and after 72 h and 7 days breaking strength was also measured. After 24-48 h of PRP and control anastomosis, increasing inflammation was observed, followed by immature granulation tissue at 72-96 h. In PRP samples, the granulation tissue was more abundant. Although breaking strength in PRP samples was higher than in control samples, differences were not statistically significant. Although differences were subtle, PRP application on intestinal anastomosis seemed to slightly improve the anastomotic resistance due to an increase of inflammatory infiltrates and fibrosis.
In this study we also determinate the platelet activation using a mean platelet component (MPC), we monitored MPC values on citrated whole blood and on PRP in all (47) animals used in this study obtained on (ADVIA 120) (a decrease in the MPC value occurs when platelets are activated and degranulated in vitro). The MPC values were not statistically significantly different (p<0.05) on citrated whole blood (21,3±1,58 g/dL) when compared to PRP (20,3±1,39 g/dL) values. In our opinion, this finding might be the result of the non significant platelets activation during PRP preparation process.
We analyzed the amount of TGF-β1 by enzyme-linked immunosorbent sandwich assay (ELISA) on PRP (obtained after double spin centrifugation), PPP (supernatant after second centrifugation) and PRP gel (obtained by mixing fresh PRP and calcium chloride). In our study, concentrations of TGF-β1 on PRP fractions were different. The concentration of TGF-β1 on PRP (24312 pg/mL) was significantly increased compared to PPP (3715 pg/mL). This supposes that the protocol for PRP obtention using a double spin centrifugation procedure employed in this study results in a high platelet concentrate and maintains a high quality biological product as measured by MPC values and TGF-β1 levels.
Keywords: intestinal anastomosis, pig, platelets, platelet-rich plasma, growth factors.
Heydenrych, Leonard Goussárd. "Eviscerated corneas as tissue source for ex vivo expansion of limbal epithelial cells on platelet-rich plasma gels." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/22923.
Повний текст джерелаTurner, Lisa A. "Profiling Precursor Lipids for Specialized Pro-Resolution Molecules in Platelet-Rich Plasma Following Fish Oil and Aspirin Intake." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4763.
Повний текст джерелаSeidel, Sarah Raphaela Torquato. "Correlação entre concentrações plaquetárias e de fator de crescimento TGF-β presente em plasma rico em plaquetas de equinos." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/10/10136/tde-23112017-131614/.
Повний текст джерелаBlood derived products have been used in equine medicine with increasing frequency, being characterized as an autologous product, with greater amount of growth factors and be capable of improvement the healing capacity in tissues with poor blood supply, such as tendons and joints, reducing the time of recovery of the animal. It is known that the growth factors are derived from platelets, but the positive correlation between the increase in platelet count and the higher concentration of growth factors is still a reason for discussion among the authors. In order to obtain a final product with a higher platelet count, it is frequent to increase the speed or number of centrifugations in the methodology employed, increasing the risk of early platelet aggregation. The aim of the present study is to verify the effect of double centrifugation in PRP preparation by comparing platelet counts, TGF-β1 growth factor concentrations, and degree of platelet activation through percentage of aggregation. Twelve horses, male, aged 3 to 5 years-old, clinically healthy were subjected. Two different protocols were performed: one with single centrifugation and the other with double centrifugation. In the first one, the anticoagulated blood was centrifugated at 141G/12 minutes; while in the second one the first centrifugation was 300G/5 minutes followed by 700G/15 minutes, with rest between them and after. The products obtained after each centrifugation were submitted to platelet counting, aggregation test and measurement of TGF-β1 by ELISA kit. The results showed a higher platelet concentration when double centrifugation protocol was used. The aggregometry test evidenced a greater activation of the platelets during the preparation of PRP when submitted to higher centrifugation velocities (times g), and not to double centrifugation. Quantification of TGF-β1 showed no difference when performed on samples with only one centrifugation, but was higher values in the final product of the second centrifugation. The determination coefficient and Pearsons correlation coefficient showed a positive correlation between the platelet count and TGF-β1 concentration. The double centrifugation protocol proved to be more effective at concentrating platelets and consequently higher amounts of TGF-β1, not being impaired by early activation during obtainment.
Assunção, Luciana Reichert da Silva. "Resposta tecidual de dentes reimplantados utilizando plasma pobre em plaquetas, plasma rico em plaquetas e células-tronco da medula óssea : estudo em cães /." Araçatuba :, 2009. http://hdl.handle.net/11449/104224.
Повний текст джерелаCoorientador: Sandra Helena Penha de Oliveira
Banca: Robson Frederico Cunha
Banca: Celso Koogi Sonoda
Banca: Elenice Deffune
Banca: Saul Martins de Paiva
Resumo: A avulsão dentária é um tipo de trauma que resulta na perda da continuidade de um complexo e múltiplo compartimento de tecidos. Componentes derivados do sangue, incluindo o plasma pobre em plaquetas (PPP) e o plasma rico em plaquetas (PRP) constituem uma opção terapêutica para o reparo tecidual. O gel de PRP tem sido usado como arcabouço para células-tronco da medula óssea em Engenharia Tecidual. O propósito da presente investigação foi avaliar o efeito do PPP, PRP ativado ou não por trombina e células-tronco (CT) originárias da medula óssea associadas ao PRP no reparo de reimplantes dentários. Quatro cães foram utilizados neste estudo. PRP e PPP foram obtidos pelo método de dupla centrifugação a partir do sangue coletado da veia jugular dos animais. CT foram obtidas por aspiração da medula óssea (AMO) e isoladas pelo método de centrifugação por densidade de gradiente. A citometria de fluxo (CF) foi realizada após AMO utilizando marcadores celulares CD34 e CD90. Após 30 minutos da extração, os dentes foram reimplantados e, de acordo com a proposta metodológica, divididos em 5 grupos: Grupo I - dentes reimplantados sem material (Controle); Grupo II - dentes reimplantados com o Plasma Pobre em Plaquetas (PPP); Grupo III - dentes reimplantados com o Plasma Rico em Plaquetas ativado por cloreto de cálcio (PRP); Grupo IV - dentes reimplantados com o Plasma Rico em Plaquetas ativado por trombina de origem bovina e cloreto de cálcio (PRP + TR) e Grupo V - dentes reimplantados com células-tronco da medula óssea associadas ao PRP ativado por cloreto de cálcio (CT + PRP). Análises histológica, histomorfométrica e imuno histoquímica foram realizadas 120 dias após o reimplante. Análise de variância (ANOVA) e o teste post-hoc de Tukey foram utilizados para a análise estatística, com nível de significância de 5%. CF mostrou... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Dental avulsion constitutes a traumatism which results in the loss of a complex and multiple tissue compartments. Blood-components products, including platelet-poor plasma (PPP) and platelet-rich plasma (PRP), constitute a therapeutic option for tissue healing. PRP gel has also been used as a scaffold for bone marrow stem cells in Tissue Engineering. This study aimed to evaluate the effect of PPP, PRP activated or not with thrombin and bone marrow stem cells (BMSC) associated with PRP on the healing of replanted teeth. Four adult hybrid dogs were used in this study. PRP and PPP were obtained trough double centrifugation technique of blood collected from the jugular vein of the animals. CTM were obtained from bone marrow aspiration (BMA) and isolated by density gradient centrifugation method. Flow Cytometry Analysis (FCA) was performed after BMA using CD34 and CD90 cells markers. After 30 minutes of extraction, teeth were replanted and, according to the methodological proposal, divided into 5 Groups: Group I - teeth replanted without any material (Control); Group II - teeth replanted with Platelet-poor Plasma (PPP); Group III - teeth replanted with Platelet-rich plasma activated by sodium chloride (PRP); Group IV - teeth replanted with Platelet-rich plasma activated by bovine thrombin and sodium chloride (PRP + TR) and Group V - teeth replanted with bone marrow stem cells associated with PRP activated by sodium chloride (BMSC + PRP). Histological, histomorphometric and immunohistochemical analysis were assessed 120 days after reimplantation. Analysis of variance (ANOVA) and Tukey post-hoc test were used for the statistical analysis, with the level of significance set at 5%. FCA showed 55.98% of CD34+ and 33.28% of CD90+ after BMA. Group V (BMSC + PRP) presented the largest areas of replacement resorption, confirmed... (Complete abstract click electronic access below)
Doutor
Yamada, Ana Lúcia Miluzzi. "Efeito do implante autólogo de plasma rico em plaquetas (PRP) e células tronco mesenquimais na reparação de lesões condrais articulares induzidas experimentalmente em equinos /." Botucatu : [s.n.], 2011. http://hdl.handle.net/11449/88981.
Повний текст джерелаAbstract: Articular cartilage defects represent a challenge for Veterinary Medicine due to the limited intrinsic potential of repair. Mesenchymal Stem Cells (MSC) and Platelet-Rich Plasma (PRP) have been used in cartilage repair to promote immunomodulation, chemotaxis, to decrease the degradation of articular cartilage and to take antiinflammatory effect. This work aims to study the use of intralesional MSC and the PRP in the treatment of articular cartilage defects induced experimentally in horses, noting the benefits and challenges encountered in implementing clinical-surgical in horses. For this purpose, both patellofemoral joints of eight animals were approached by arthroscopic surgery to perform a cartilage defect, on the medial femoral trochlea. After 30 days of induction the animals, who were divided into four groups, received treatment with: MSC (Group 1), PRP (Group 2), MSC and PRP (Group 3) and a control without treatment (Group 4). The clinical and laboratory assessments were performed every 15 days in 120 days, with last analysis in day 150. The macroscopic and morphologic analyses were performed at 0 and 150 day. There were clinical and laboratory improvement in the treated groups compared with control group. The macroscopic and morphological analysis showed a better tissue repair in the treated groups, mainly in groups 2 and 3. Thus, the implantation of autologous MSC and PRP showed beneficial effects in the treatment of chondral lesions, experimentally induced in horses
Orientador: Ana Liz Garcia Alves
Coorientador: Marcos Jun Watanabe
Banca: Ligia Souza Lima Silveira da Mota
Banca: Raquel Yvone Arantes Baccarin
Mestre
Akingboye, Akinfemi A. "Elucidating the biological role of autologous derived platelet-rich plasma gel in the treatment of chronic diabetic foot ulcers." Thesis, Queen Mary, University of London, 2012. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8306.
Повний текст джерелаPazzini, Josiane Morais. "Uso de esponja cirúrgica em enxertos cutâneos associado ao plasma rico em plaquetas em coelhos (Oryctolagus cuniculus) /." Jaboticabal, 2017. http://hdl.handle.net/11449/150128.
Повний текст джерелаCoorientador: Paola Castro Moraes
Banca: Rafael Ricardo Huppes
Banca: Jorge Luiz Costa Castro
Banca: Bruno Watanabe Minto
Banca: Pamela Rodrigues Reina Moreira
Resumo: Enxertos cutâneos são segmentos da epiderme e derme completamente removido da região doadora e transferidos para o local receptor, sem a presença de pedículo vascular. O curativo compressivo é recomendado por otimizar o contato do enxerto com o leito da ferida. O sucesso da cicatrização dos enxertos depende de adequada angiogênese, e por serem desprovidos de pedículo vascular o procedimento cirúrgico pode ser comprometido. Assim, para evitar complicações o plasma rico em plaquetas (PRP) é uma substância que estimula a angiogênese e auxilia na reparação tecidual. Sendo assim, delineou-se um estudo a fim de avaliar a eficácia do gel de PRP associado com esponjas cirúrgicas favorecerem a integração do enxerto ao leito receptor nos modelos de enxertos em camada e malha. Foi realizado no Hospital Veterinário da UNESP, câmpus de Jaboticabal - SP, um estudo com 64 coelhos, separados em oito grupos, com oito animais, todos submetidos à técnica de cirurgia reconstrutiva para confecção de enxerto. Os grupos foram compreendidos em: Gprpc (gel de PRP sem associação da esponja cirúrgica no enxerto de camada), Gprpce (gel de PRP associado com esponja cirúrgica como forma de curativo no enxerto de camada), Gcc (solução fisiológica 0,9% sem associação da esponja cirúrgica no enxerto de camada), Gcce (solução fisiológica 0,9% associada com esponjas cirúrgicas no enxerto de camada), Gprpm (gel de plasma PRP sem associação da esponja cirúrgica no enxerto de malha), Gprpme (gel de PRP associado ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Skin grafts are segments of the epidermis and dermis completely removed from the donor region and transferred to the receptor site without the presence of vascular pedicle. Pressure dressing after surgery is recommended to optimize the contact of the graft to the wound bed, and allow proper angiogenesis. The success of the healing of the grafts depends on proper angiogenesis, and being devoid of vascular pedicle the surgical procedure may be compromised. Thus, to avoid complications platelet rich plasma (PRP) is a substance that has been investigated for having angiogenesis factors which stimulate and aid in tissue repair. Thus, it outlined a study to assess the efficacy of PRP gel employment associated with surgical sponges to improve the integration of the graft in the recipient bed models grafts mesh and tier. It was held at the Veterinary Hospital UNESP, campus of Jaboticabal - SP, a study of 64 rabbits, divided into eight groups with eight animals, all underwent reconstructive surgery technique for making graft. The groups were comprised of: Gprpc received PRP gel unassociated surgical sponge in the tier graft, Gprpce received PRP gel associated with surgical sponge as a way of dressing in tier graft, Gcc received 0.9% saline without association surgical sponge in tier graft, Gcce received 0.9% saline solution associated with surgical sponges in the tier graft, Gprpm received plasma PRP gel unassociated surgical sponge in mesh graft, Gprpme received PRP gel associated with surgical sponge as way of dressing in mesh graft, Gcm receive 0.9% saline solution without associated surgical sponge in mesh graft and Gcce receive 0.9% saline solution associated with surgical sponges in mesh graft. Evaluations were performed macroscopic lesions after three, seven, and 14 days of surgery. To evaluate the wound healing took place microscopic ev... (Complete abstract click electronic access below)
Doutor
Gaus, Moritz [Verfasser]. "Kontrollierte Studie zur Wirkung von thrombozytenreichem Plasma (platelet rich plasma, PRP) auf den Heilungsverlauf von natürlich enstandenen Tendopathien der oberflächlichen Beugesehne des Pferdes / Moritz Gaus." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2016. http://d-nb.info/1104404141/34.
Повний текст джерелаHofmann, Katharina [Verfasser]. "Verfahren zur automatisierten Herstellung von autologen Blutderivaten (Platelet Rich Plasma) mit dem Ziel der Behandlung von chronischen Wunden / Katharina Hofmann." München : Verlag Dr. Hut, 2020. http://d-nb.info/1219475661/34.
Повний текст джерелаAustin, Brittany Logan. "A Biomechanical Investigation of Collagen, Platelet-rich Plasma, and Mesenchymal Stromal Cells on the Achilles Tendon in a Rat Model." Youngstown State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ysu155905140840335.
Повний текст джерелаCampos, Natália de. "Influência do plasma rico em plaquetas derivado do aspirado de medula óssea na cicatrização de defeitos periodontais : estudo histológico, histométrico e imunoistoquímico em ratos /." Araçatuba, 2014. http://hdl.handle.net/11449/123401.
Повний текст джерелаBanca: Valdir Gouveia Garcia
Banca: Alvaro Francisco Bosco
Banca: Joni Augusto Cirelli
Banca: Luciana Saraiva
Resumo: Este estudo avaliou a influência do plasma rico em plaquetas derivado do aspirado de medula óssea (PRP-bma) na cicatrização de defeitos de fenestração periodontal (PFD) em ratos. PFD foram criados cirurgicamente na mandíbula de 40 ratos. Os animais foram divididos randomicamente em 2 grupos: C e PRP-bma - os defeitos foram preenchidos com coágulo sanguíneo ou PRP-bma, respectivamente. Os animais foram submetidos à eutanásia aos 10 ou 30 dias pós-operatórios. Foram realizadas análises histológica, histométrica e imunoistoquímica. A porcentagem de novo osso (NB), densidade do novo osso formado (DNB), novo cemento (NC) e extensão do defeito remanescente (ERD) foram avaliados histometricamente. Foram realizadas reações imunoistoquímicas para detecção de antígeno nuclear de proliferação celular (PCNA), sialoproteína óssea (BSP), osteocalcina (OCN) e fosfatase ácida resistente ao tartarato (TRAP). Células imunomarcadas foram quantificadas. Os dados foram analisados estatisticamente (ANOVA; Tukey, P < 0,05). Aos 10 dias, os grupos C e PRP-bma apresentaram quantidades similares de NB e DNB, e não foi observada formação de NC. Aos 30 dias, os grupos C e PRP-bma apresentaram quantidades similares de NB e DNB; o Grupo PRP-bma mostrou significativa formação de NC, com fibras colágenas inseridas obliquamente ou perpendicularmente à superfície radicular, sendo que nenhum espécime do Grupo C apresentou formação de NC. O Grupo PRP-bma apresentou um número significativamente maior de células PCNA-postivas (aos 10 dias) e BSP-positivas (aos 10 e 30 dias) que o Grupo C. Não foram observadas diferenças significativas no número de células OCN-positivas ou TRAP-positivas entre os grupos aos 10 ou 30 dias. Pode-se concluir que o PRP-bma promoveu formação de NC com ligamento periodontal funcional
Abstract: This study evaluated the influence of platelet-rich plasma derived from bone marrow aspirate (PRP-bma) on the healing of periodontal fenestration defects (PFD) in rats. PFD were surgically created in the mandible of 40 rats. The animals were randomly divided into 2 groups: C and PRP-bma - defects were filled with blood clot or PRP-bma, respectively. Animals were euthanized at either 10 or 30 days post-operative. Histologic, histometric and immunohistochemical analyses were performed. Percentage of new bone (NB), density of newly formed bone (DNB), new cementum (NC) and extension of remaining defect (ERD) were histometrically evaluated. Proliferating cell nuclear antigen (PCNA), bone sialoprotein (BSP), osteocalcin (OCN), and tartrate-resistant acid phosphatase (TRAP) immunohistochemical staining were performed. Immunolabeled cells were quantified. Data were statistically analyzed (ANOVA; Tukey, P < 0.05). At 10 days, groups C and PRP-bma presented similar amounts of NB and DNB, NC formation was not observed. At 30 days, groups C and PRP-bma presented similar amounts of NB and DNB; Group PRP-bma showed significant NC formation, with collagen fibers inserted obliquely or perpendicularly to the root surface, while NC formation was not observed in any Group C specimen. Group PRP-bma presented a significantly higher number of PCNA-positive (at 10 days) and BSP-positive cells (at 10 and 30 days) than Group C. No significant differences in the number of either OCN-positive or TRAP-positive cells were observed between groups at 10 or 30 days. It can be concluded that PRP-bma promoted NC formation with a functional periodontal ligament
Doutor
Danieli, Marcus Vinicius [UNESP]. "Lesão condral do joelho: comparação entre ressonância magnética e vídeo-artroscopia. Efeito da aplicação do plasma rico em plaquetas." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/136457.
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A estrutura da cartilagem hialina é muito complexa e por possuir poucas células e não ter vasos sanguíneos, linfáticos ou nervos, tem seu potencial de cicatrização muito limitado. Lesões da cartilagem hialina do joelho são muito comuns, e seu tratamento representa um grande desafio. As opções cirúrgicas disponíveis atualmente para essa lesão, como condroplastia, microfraturas, mosaicoplastia ou transplante autólogo de condrócitos, ainda não tem resultados satisfatórios, principalmente em longo prazo. O Plasma Rico em Plaquetas (PRP) vem sendo usado na ortopedia desde os anos 90 para estimular a cicatrização dos tecidos, devido ao seu potencial de concentrar fatores de crescimento no local desejado. O objetivo da aplicação do PRP seria estimular um melhor ambiente de cicatrização. Em cartilagem o PRP tem sido usado no tratamento de osteoartrose e como coadjuvante em técnicas de tratamento de lesões condrais. Porém a literatura ainda apresenta resultados duvidosos em relação a aplicação do PRP em cirurgias de cartilagem. Essa tese apresenta a aplicação cirúrgica do PRP em pacientes com lesões condrais de joelhos, para avaliar se esse tratamento pode ter o efeito de acelerar e/ou melhorar o resultado cirúrgico destes pacientes. A tese foi dividida em três partes, sendo a primeira a revisão da literatura sobre tratamento de lesões condrais e sobre o plasma rico em plaquetas em ortopedia, a segunda parte um artigo sobre a comparação da avaliação das lesões condrais do joelho pela artroscopia comparada à ressonância nuclear magnética e a terceira e última parte é o trabalho principal que analisa o efeito da aplicação cirúrgica do PRP em lesões condrais do joelho.
The hyaline cartilage structure is very complex, with few cells, and without blood and lymphatic vessels or nerves. This makes the healing potential very limited. Knee cartilage injuries are very common, and its treatment is a major challenge. Surgical options available nowadays like chondroplasty, microfractures, mosaicplasty and autologous chondrocyte transplantation still doesn’t have satisfactory results, mainly in long term. Platelet-Rich Plasma (PRP) has been used in orthopedics since the 90’s in order to stimulate tissue healing, because of its potential to concentrate platelet derived growth factors in the target place. The goal of the PRP application is to stimulate a better healing environment. PRP has been used in cartilage to treat osteoarthritis and to support treatment techniques for chondral injuries. However, the literature is still doubtful regarding the surgical results with PRP application. This thesis presents the PRP application in patients with knee chondral injuries to evaluate if this treatment is able to accelerate the healing process and/or to improve the surgical results in these patients. The thesis was divided into three parts, the first one is a literature review about chondral injuries treatments and platelet-rich plasma in orthopedics; the second part is a manuscript comparing the chondral injuries evaluation by arthroscopy and magnetic resonance imaging; and the last part is the main article that analyses the effect of PRP surgical application in knee chondral injuries.
Danieli, Marcus Vinicius. "Lesão condral do joelho comparação entre ressonância magnética e vídeo-artroscopia. Efeito da aplicação do plasma rico em plaquetas /." Botucatu, 2016. http://hdl.handle.net/11449/136457.
Повний текст джерелаCoorientador: Hamilton da Rosa Pereira
Resumo: A estrutura da cartilagem hialina é muito complexa e por possuir poucas células e não ter vasos sanguíneos, linfáticos ou nervos, tem seu potencial de cicatrização muito limitado. Lesões da cartilagem hialina do joelho são muito comuns, e seu tratamento representa um grande desafio. As opções cirúrgicas disponíveis atualmente para essa lesão, como condroplastia, microfraturas, mosaicoplastia ou transplante autólogo de condrócitos, ainda não tem resultados satisfatórios, principalmente em longo prazo. O Plasma Rico em Plaquetas (PRP) vem sendo usado na ortopedia desde os anos 90 para estimular a cicatrização dos tecidos, devido ao seu potencial de concentrar fatores de crescimento no local desejado. O objetivo da aplicação do PRP seria estimular um melhor ambiente de cicatrização. Em cartilagem o PRP tem sido usado no tratamento de osteoartrose e como coadjuvante em técnicas de tratamento de lesões condrais. Porém a literatura ainda apresenta resultados duvidosos em relação a aplicação do PRP em cirurgias de cartilagem. Essa tese apresenta a aplicação cirúrgica do PRP em pacientes com lesões condrais de joelhos, para avaliar se esse tratamento pode ter o efeito de acelerar e/ou melhorar o resultado cirúrgico destes pacientes. A tese foi dividida em três partes, sendo a primeira a revisão da literatura sobre tratamento de lesões condrais e sobre o plasma rico em plaquetas em ortopedia, a segunda parte um artigo sobre a comparação da avaliação das lesões condrais do joelho pela ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The hyaline cartilage structure is very complex, with few cells, and without blood and lymphatic vessels or nerves. This makes the healing potential very limited. Knee cartilage injuries are very common, and its treatment is a major challenge. Surgical options available nowadays like chondroplasty, microfractures, mosaicplasty and autologous chondrocyte transplantation still doesn't have satisfactory results, mainly in long term. Platelet-Rich Plasma (PRP) has been used in orthopedics since the 90's in order to stimulate tissue healing, because of its potential to concentrate platelet derived growth factors in the target place. The goal of the PRP application is to stimulate a better healing environment. PRP has been used in cartilage to treat osteoarthritis and to support treatment techniques for chondral injuries. However, the literature is still doubtful regarding the surgical results with PRP application. This thesis presents the PRP application in patients with knee chondral injuries to evaluate if this treatment is able to accelerate the healing process and/or to improve the surgical results in these patients. The thesis was divided into three parts, the first one is a literature review about chondral injuries treatments and platelet-rich plasma in orthopedics; the second part is a manuscript comparing the chondral injuries evaluation by arthroscopy and magnetic resonance imaging; and the last part is the main article that analyses the effect of PRP surgical application... (Complete abstract click electronic access below)
Doutor
Samarkanova, Dinara. "Umbilical Cord Blood Platelet Rich Plasma derivatives for therapeutic applications and development of clinical trials for treatment of skin and ocular ulcers." Doctoral thesis, Universitat Autònoma de Barcelona, 2020. http://hdl.handle.net/10803/670817.
Повний текст джерела"Entre las ventajas en el uso de la sangre del cordón (CB) como fuente de plaquetas y del plasma para la medicina regenerativa se destacan la disponibilidad, el uso universal, los donantes jóvenes y el material biológico viralmente seguro, rico en factores regenerativos. Los derivados del plasma rico en plaquetas de cordón (CB-PRP) se han investigado como posibles agentes terapéuticos para el tratamiento de diversas afecciones, entre ellas las enfermedades de la superficie ocular y las úlceras de la piel. El objetivo de esta tesis es validar un diseño de bioproceso para la producción de concentrados de plaquetas derivados de cordón (CBPC) en un banco de cordón público (método BST). El CBPC se definió como un producto de 10±5mL, 1.000±200×10E9plaquetas/L. Un total de 300 unidades de cordón fueron centrifugadas en dos pasos para concentrar las plaquetas, en condiciones para cumplimiento de GMP. Las CBPC se activaron después de la descongelación con un 10% de gluconato de calcio para generar geles de plaquetas (CBPG) para tratar a los pacientes con úlceras de pie diabético. Además, las características moleculares de los factores tróficos, angiogénicos y citoquinas de diferentes preparados derivados del CB-PRP (plasma pobre en plaquetas: CB-PPP; lisado de plaquetas: CB-PL y los factores obtenidos a partir de liberación de plaquetas activadas: CB-PR) se evaluaron para determinar su aplicación clínica más apropiada sobre la base de los perfiles funcionales e inmunomoduladores. Por último, se realizó un estudio de casos en el que se evaluaron 46 ojos que no respondían a los tratamientos convencionales y que requerían intervenciones urgentes, y que recibieron colirio alogénico obtenido del lisado de plaquetas de la sangre del cordón umbilical (CBED) para tratar lesiones graves de la superficie ocular en uso compasivo. Un total del 84% de las unidades de CB procesadas cumplieron los criterios de aceptación. Los productos finales contenían 1.017±149×10E9plaquetas/L en 10±3mL. Para la evaluación clínica, se aplicó un total de 21 CBPG en 3 pacientes, sin que se reportaran eventos adversos y con una mejoría de las úlceras en todos ellos. Además, se investigó la función inmunomoduladora de los derivados del CB-PRP, para lo cual se utilizaron PBMC adultas con preparados de CB-PRP que redujeron drásticamente la activación de las células NK, NKT y T. De los tres preparados CB-PRP que se investigaron, CB-PL y CB-PR tienen concentraciones más altas de factores tróficos (EGF,bFGF,HGF,TGF-β1) y pro-angiogénicos (VEGF,PDGF-AB/BB,MMP-2,9,TIMP1-4), el CB-PPP tiene la concentración más baja de todos los analitos medidos. Sobre la base de estos resultados, el CB-PR parece ser el material de partida más adecuado para los parches de las heridas de la piel, mientras que el CB-PL y el PPP podrían utilizarse como colirios para patologías graves de la superficie ocular (ulceras) y condiciones inflamatorias (ojo seco severo). Además, evaluamos la aplicación clínica en el tratamiento compasivo en los grupos de pacientes con úlceras corneales que mostraron una recuperación total (78%) y parcial (19%) de la úlcera. Un ojo (3%) no respondió al tratamiento. Para los grupos con condiciones crónicas se reportó una mejoría en 85%, y las lesiones empeoraron con el tratamiento en ambos ojos (15%) de un paciente. No se atribuyeron eventos adversos severos directamente al CBED. La producción de CBPC es válido y reproducible. Los derivados del CB-PRP son ricos en factores tróficos y angiogénicos; con capacidad de inmunosupresión. La disponibilidad inmediata de CBPG y CBED dio lugar a un tratamiento alogénico bien-tolerado que mostró signos de seguridad y eficacia en los pacientes tratados. Estos resultados positivos respaldan ampliación clínica de CBPG y CBED como nuevos productos medicinales de los bancos de cordón.
There are many advantages to using cord blood (CB) as a source of therapeutic platelet and plasma derivatives for regenerative medicine. These include availability, universal use, young donor source, and virally safe biological material, rich in tissue regenerative factors. CB platelet rich plasma (CB-PRP) derivatives have been investigated as potential therapeutic agents for the treatment of diverse conditions including ocular surface disease and skin ulcers The aim of this thesis is to validate a bioprocess design for the production of cord blood-derived platelet concentrates (CBPC) in a public CB Bank (BST method). CBPC was defined as a product of 10±5 mL, 1,000±200×10E9/L total platelets, free of erythrocytes and leukocytes. A total of 300 CB units were centrifuged in two steps to enrich for platelets, in compliance with GMP. The samples were tested for the degree of platelet activation present, and the levels of growth factor were analyzed to evaluate their function. CBPC were then activated after thawing with 10% calcium gluconate to generate platelet gels (CBPG) to treat patients with diabetic foot ulcers. Additionally, the molecular characteristics for trophic, angiogenic factors and cytokines of different preparations derived from CB-PRP (platelet poor plasma: CB-PPP; platelet lysate: CB-PL; platelet releasate: CB-PR) were assessed to evaluate their most appropriate clinical application based on functional and immunomodulatory profiles. Finally, a case study evaluating 46 eyes unresponsive to conventional treatments and requiring urgent interventions, who received allogeneic eye drops obtained from CB-PL (CBED) to treat severe ocular surface lesions under compassionate use was performed A total of 84% of the processed CB units fulfilled the acceptance criteria. Final products contained 1,017±149×10E9 platelets/L in 10±3mL of plasma. Platelet recovery was 50±9%. BST method ensures depletion of white and red blood cells. Platelets showed low levels of activation during processing, but were significantly activated after thawing, further test shows that product are rich in growth factors. For clinical evaluation, a total of 21 CBPG were applied in 3 patients, with no reported adverse events and improvement of ulcers in all of them. Further was investigated the immunomodulatory function of CB-PRP derivatives, for this was used adult PBMCs with CB-PRP preparations that dramatically reduced the activation of NK, NKT and T cells. Of the three preparations CB-PRP that were investigated, CB-PL and CB-PR have higher concentrations of trophic (EGF, bFGF, HGF, TGF-β1) and pro-angiogenic (VEGF, PDGF AB/BB, MMP-2,9, TIMP1-4) factors, CB-PPP has the lowest concentration of all measured analytes. Based on these findings CB-PR seems the most suitable starting material for skin wound patches, while CB-PL and PPP could be used to prepare eye drops for severe corneal pathologies and inflammatory conditions such as ulcers or sever dry eye disease, respectively. Additionally, we evaluated clinical application in compassionate treatment on patients with ocular surface disorders in groups with corneal ulcers (neurotrophic, trauma, burns) which showed full and partial ulcer recovery in 25 (78%) and 6 (19%) eyes respectively. One eye (3%) did not respond to treatment. For groups with chronic conditions (dry eye and oGVHD) improvement was reported in 12 (85%) eyes, and lesions worsened on treatment in both eyes (15%) of one patient. No severe adverse events were directly attributed to CBED BST method for CBPC production is valid and reproducible, and CB-PRP derivatives are rich in trophic and angiogenic factors; with immunosuppression capacity. Promptly available CBPG and CBED resulted in a well-tolerated allogeneic therapy that showed evidence of safety and efficacy in treated patients. These positive results support final steps of clinical scale-up of both CBPG and CBED as a novel medicinal products of CB Banks.
Vanni, Isabele Silveira Rosa [UNESP]. "Obtenção, indicadores de qualidade e propriedades dos hormônios derivados de plaquetas humanas pela técnica de Lisado Plaquetário." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/142841.
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Muitas especialidades médicas têm utilizado o Plasma Rico em Plaquetas (PRP) em diferentes modalidades terapêuticas, como está ocorrendo com a Ortopedia e em Cirurgia Plástica. No entanto, a denominação utilizada para o PRP é muitas vezes equivocada. Diante do crescente número de especialidades médicas usando PRP ou Hormônios Derivados de Plaquetas (HDP) este trabalho foi delineado. Objetivo: compreende três etapas: 1) Levantamento bibliográfico em base de dados com a palavra-chave: platelet rich plasma com intuito de avaliar criticamente os artigos publicados na literatura em revistas com Fator de Impacto (FI)≥1; 2) Obter PRP, Concentrado de Plaquetas (CP) e HDP de indivíduos saudáveis, pela técnica de lisado plaquetário, após a utilização de agonista e congelamento / descongelamento (N=10) e 3) Avaliar o desempenho destes preparados como substituto do Soro Fetal Bovino na cultura de Células Tronco Mesenquimais humanas (CTMh). Casuística e Métodos: Os indicadores monitorados foram: idade, sexo, tipagem ABO/RhD, fenotipagem eritrocitária Rh, Kell e determinação de fatores de crescimento pelo sistema Multiplex-Milliplex®: PDGF- AA, RANTES/CCL5, PAI-1 (total), VEGF-A, FGF-1/FGF-ácido, FGF-2/FGF-básico, EGF, Angiopoietina-2, Fibrinogênio e Fator von Willebrand, estes dosados nas 3 preparações: PRP, CP e HDP. Todos os indicadores monitorados foram realizados análise estatística. Resultados: Foram analisados 50 artigos entre 2012-2016, destes, 29 com a palavra-chave platelet-rich plasma, 14 platelet lysate (PL) e 7 platelet growth factor (PGF). Os artigos publicados com a palavra-chave PL são de periódicos com maior FI e maior coerência com a metodologia, seguida do PGF e PRP. Contata-se que a maioria dos trabalhos com a terminologia PRP foi empregada de forma equivocada. Estes indicadores registram que não existe correlação entre a contagem plaquetária e a dosagem de hormônios. A idade dos indivíduos está inversamente relacionada com a concentração de fator, à exceção do FGF. Não há correlação da quantidade de fatores de crescimento e sexo. Indivíduos do grupo sanguíneo A, e fenótipo ee são melhores secretores de hormônios de crescimento. Quando se comparam os 3 métodos analisados, o CP e o HDP são estatisticamente superiores ao PRP quanto à dosagem de fatores de crescimento. A análise desempenho dos preparados, na concentração de 20% quando comparado com o Soro Fetal Bovino na mesma concentração, identifica que o melhor desempenho quantitativamente foi CP>PRP>HDP=CTLE. No entanto a análise histológica evidencia um grande número de células aderidas ao scaffold de fibrina, com desempenho nitidamente superior para o HDP. Conclusão: A maioria dos trabalhos publicados usa a terminologia PRP incorretamente. Levando-se em consideração a quantificação dos hormônios plaquetários dosados e o desempenho em cultura celular as melhores técnicas são CP e HDP, ficando o PRP em desvantagem.
Diversity medical specialties have been using Platelet Rich Plasma (PRP) therapy form as is happening with the Orthopedics and Plastic Surgery. However, the name used for the PRP is often used wrongly. This work was outlined considering the growing number of medical specialties using PRP or platelet-derived hormones (PDH). Objective: The work consisted of three steps: 1) Bibliographic search with the key word PRP in order to evaluate the articles published in journals with Impact Factor (IF) ≥1; 2) obtaining of PRP, platelet concentrate (PC) and PDH from healthy individuals using the platelet lysate technique after use agonist and freeze / thaw (N = 10); 3) evaluate the performance of these preparations as substitute for fetal bovine serum in culture of human mesenchymal stem cells. Methods: The indicators monitored were: age, sex, ABO / RhD, erythrocyte phenotyping Rh, Kell and determination of growth factors by Multiplex-Milliplex® system(PDGF-AA, RANTES / CCL5, PAI-1 (total), VEGF-A, FGF-1 / FGF-acid, FGF-2 / FGF-basic, EGF, Angiopoietin-2, fibrinogen and Factor von Wilebrand. The monitored indicators were performed statistical analysis. Results: These parameters were measured in three preparations: PRP, PC and PDH. It was analyzed 50 articles between 2012-2016, of these, 29 with the platelet-rich plasma password, 14 platelet lysate (PL) 7 and platelet growth factor (PGF). Articles published with the keyword PL are journals with higher IF and greater consistency with the methodology followed by PGF and last PRP. It was observed that most of the work with the PRP terminology was used wrongly. These indicators showed that there is no correlation between the platelet count and the dosage of hormones. The age of individuals is inversely related with the concentration of factors, excepting FGF. There is no correlation between the amount of growth factors and gender. Individuals belonging to blood group A and no phenotype E are better secreting of growth hormones. When comparing the three methods analyzed, PC and PDH are statistically higher than PRP on the dosage of growth factors. Compared with 20% of Fetal Bovine Serum, the performance was quantitatively better to PC> PRP> CTL=PDH on the viability of suspension cell. However, the histological analysis showed a large number of cells attached to the fibrin scaffold with higher performance to the PDH. Conclusion: The majority of published studies using PRP terminology are incorrectly. Considering the quantitation of platelet hormones and the performance in cell culture, the best techniques are PC and PDH.
Rubina, Filipa Ascensão Alves. "A Prospective Double-Blind Placebo Controlled Study to Assess the Efficacy of Platelet-Rich Plasma on the Treatment of Androgenetic Alopecia." Doctoral thesis, Universitat Internacional de Catalunya, 2017. http://hdl.handle.net/10803/403440.
Повний текст джерелаBlumenschein, Alexandre Roriz. "Enxertos de gordura associados a plasma rico em plaquetas em ratas - estudo experimental." Universidade Federal de Goiás, 2013. http://repositorio.bc.ufg.br/tede/handle/tede/3490.
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Autologous fat grafts used for filling soft tissue defects have been used for more than a century. Fat is considered an ideal filler because of its low cost, ease of harvest, abundance in the human body and low immunogenic and allergic reaction due to its autologous nature, being largely used in aesthetic and reconstructive plastic surgery. Platelet rich plasma (PRP) is a plasma fraction, with platelet count above baseline, generally obtained via centrifugation of blood. PRP theoretically promotes tissue regeneration due to fact that it concentrates a greater amount of growth factors essential in the process of tissue regeneration and neovascularization. This paper aims to examine if the association of fat grafts and PRP improves graft viability in female rats, through an experimental, randomized and blinded study, which involved 47 animals. These animals underwent fat graft harvest from their inguinal fat deposits and fat grafting subcutaneously to their cranial region. In 22 animals the fat graft was mixed with PRP and in 25 the fat was grafted by itself. After a 100 day period, the animals were sacrificed and the fat grafts were analyzed using scores from 0 (absent) to 4 (abundant), in optical microscopy by two independent and blinded pathologists, by means of the following variables: fat graft cell viability, fat necrosis, tissue inflammation and fibrosis. Regarding fat graft cell viability, the PRP group scored moderate/abundant in 63% of the cases and the fat graft only group scored absent/slight in 72% of the cases (p<0.05). The PRP group presented lower fat necrosis scores and lower tissue inflammation scores when compared to the fat graft only group (p<0.05). The presence of tissue fibrosis was rarely observed in both groups. Tumors (dermoid cysts) within the fat grafts were observed in 3 animals in which the grafts were mixed with PRP. It is concluded that PRP improves the viability and integration of fat grafts in rats, but more studies are needed to fully understand the exact mechanisms that lead to this improvement and assess the safety of the method for use in humans.
Enxertos de gordura autóloga têm sido usados há mais de um século para preenchimento de defeitos de tecidos moles. A gordura é considerada um material de preenchimento ideal, devido ao baixo custo de obtenção, abundância no corpo e baixa reação imunogênica e alérgica, por se tratar de material autólogo, tendo amplo uso na cirurgia plástica estética e reparadora. O plasma rico em plaquetas (PRP) consiste em uma fração de plasma sanguíneo com concentração de plaquetas acima do normal, obtida geralmente através de centrifugação do sangue autólogo que separa os componentes celulares de acordo com seu peso, concentrando as plaquetas de forma seletiva. Esta fração sanguínea (PRP) teoricamente melhora a regeneração de tecidos, por conter em grande quantidade citocinas e fatores de crescimento essenciais no processo de regeneração e neovascularização de tecidos. O objetivo deste estudo foi analisar se a associação de plasma rico em plaquetas a enxertos de gordura em ratas melhora a viabilidade destes enxertos. Trata-se de uma pesquisa de delineamento experimental com animais, cego e randomizado. Foram estudados 47 ratas, submetidas à enxerto de gordura autóloga proveniente da região inguinal para a região subcutânea craniana. Vinte e duas ratas foram submetidas a enxerto de gordura autóloga associado a PRP (GCP) e vinte cincos ratas submetidos à enxerto de gordura sem plasma (GSP). Após 100 dias os animais foram sacrificados e as áreas de enxerto de gordura foram analisadas de forma independente e cega, por duas avaliadoras, de acordo com as seguintes variáveis: viabilidade da gordura enxertada, áreas de necrose, inflamação tecidual e áreas de fibrose, a partir de escores de avaliação histológica de 0 a 4 (ausente à abundante). Na avaliação da viabilidade das células gordurosas no enxerto, o GCP apresentou escore abundante em 63% dos casos e o GSP apresentou escore escasso em 72% destes (p<0,05). O GCP apresentou escores de necrose gordurosa e inflamação tecidual menor quando comparado ao GSP (p<0,05). A presença de fibrose tecidual foi pouco observada em ambos os grupos. Como achado histológico, observouse a presença de tumores (cistos dermoides) em três casos do grupo GCP. Conclui-se que o PRP melhora a viabilidade e integração de enxertos de gordura autóloga em ratas, porém novos estudos são necessários para entender completamente esse mecanismo e avaliar a segurança do método para uso em humanos.
Pazzini, Josiane Morais [UNESP]. "Plasma rico em plaquetas empregado na cirurgia reconstrutiva em coelhos (Oryctolagus cuniculus): avaliação da exequibilidade da técnica, achados macroscópicos e histopatológicos." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/110437.
Повний текст джерелаFeridas de grandes extensões, com perda da viabilidade tecidual e retardo na cicatrização por segunda intenção são casos que se faz necessário o emprego de técnicas cirúrgicas reconstrutivas. O plasma rico em plaquetas (PRP) é um produto com maior concentração plaquetária, adjuvante no processo cicatricial de cirurgias reconstrutivas, auxiliando nos processos de hemostasia e estimulação da angiogênese. Dessa forma, delineou-se um estudo a fim de avaliar a eficácia do uso do gel produzido a partir do plasma rico em plaquetas (PRP) em flapes de avanço de padrão axial toracodorsal em coelhos, para avaliar a possibilidade de favorecer a integração do retalho no leito receptor. Utilizaram-se 30 coelhos da raça Nova Zelândia branco, separados em dois grupos de 15 animais, compreendendo os grupos plasma rico em plaquetas (GPRP), na qual empregou-se o gel antes da síntese da ferida cirúrgica, e controle (GC), na qual utilizou-se apenas solução fisiológica. Para obtenção do PRP, coletou-se sangue dos animais, e determinou-se a contagem plaquetária antes da preparação do gel. No início e término do experimento os animais foram pesados para posterior análise de ganho peso médio. Após o procedimento cirúrgico iniciou-se as avaliações macroscópicas no 3º, 7º e 14º dia, e avaliou-se presença ou ausência de exsudato, integridade da pele, edema, rubor e necrose. Após esta etapa, coletou-se o material da ferida cirúrgica para confecção das lâminas histológicas e posterior avaliação microscópica. Avaliou-se a proliferação vascular, presença de células mononucleares e polimorfonucleares, proliferação fibroblástica, colagenização, reepitelização e hemorragia. Os dados obtidos foram submetidos à análise estatística (Teste t Student, t emparalhado, e Kruskall Walis, sendo p<0,05). O ganho de peso médio não foi significativo entre os grupos ...
Wounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the ...
Pazzini, Josiane Morais. "Plasma rico em plaquetas empregado na cirurgia reconstrutiva em coelhos (Oryctolagus cuniculus) : avaliação da exequibilidade da técnica, achados macroscópicos e histopatológicos /." Jaboticabal, 2014. http://hdl.handle.net/11449/110437.
Повний текст джерелаBanca: Paola Castro Moraes
Banca: Geórgia Modé Magalhães
Resumo: Feridas de grandes extensões, com perda da viabilidade tecidual e retardo na cicatrização por segunda intenção são casos que se faz necessário o emprego de técnicas cirúrgicas reconstrutivas. O plasma rico em plaquetas (PRP) é um produto com maior concentração plaquetária, adjuvante no processo cicatricial de cirurgias reconstrutivas, auxiliando nos processos de hemostasia e estimulação da angiogênese. Dessa forma, delineou-se um estudo a fim de avaliar a eficácia do uso do gel produzido a partir do plasma rico em plaquetas (PRP) em flapes de avanço de padrão axial toracodorsal em coelhos, para avaliar a possibilidade de favorecer a integração do retalho no leito receptor. Utilizaram-se 30 coelhos da raça Nova Zelândia branco, separados em dois grupos de 15 animais, compreendendo os grupos plasma rico em plaquetas (GPRP), na qual empregou-se o gel antes da síntese da ferida cirúrgica, e controle (GC), na qual utilizou-se apenas solução fisiológica. Para obtenção do PRP, coletou-se sangue dos animais, e determinou-se a contagem plaquetária antes da preparação do gel. No início e término do experimento os animais foram pesados para posterior análise de ganho peso médio. Após o procedimento cirúrgico iniciou-se as avaliações macroscópicas no 3º, 7º e 14º dia, e avaliou-se presença ou ausência de exsudato, integridade da pele, edema, rubor e necrose. Após esta etapa, coletou-se o material da ferida cirúrgica para confecção das lâminas histológicas e posterior avaliação microscópica. Avaliou-se a proliferação vascular, presença de células mononucleares e polimorfonucleares, proliferação fibroblástica, colagenização, reepitelização e hemorragia. Os dados obtidos foram submetidos à análise estatística (Teste t Student, t emparalhado, e Kruskall Walis, sendo p<0,05). O ganho de peso médio não foi significativo entre os grupos ...
Abstract: Wounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the ...
Mestre
Mizuno, Hirokazu, Hideaki Kagami, Junji Mase, Daiki Mizuno, and Minoru Ueda. "Efficacy of Membranous Cultured Periosteum for the Treatment of Patients with Severe Periodontitis: a Proof-of-Concept Study." Nagoya University School of Medicine, 2010. http://hdl.handle.net/2237/12910.
Повний текст джерелаGhassab, Sasan. "Thromboelastographic Clot Parameters of Autologous Equine Blood Products Activated by Various Clotting Agents." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397432210.
Повний текст джерелаTeixeira, Adelaide de Aguiar Marques. "Otimização da obtenção de plasma rico em plaquetas no gato." Master's thesis, Universidade de Lisboa. Faculdade de Medicina Veterinária, 2014. http://hdl.handle.net/10400.5/7729.
Повний текст джерелаA aplicação de plasma rico em plaquetas (PRP) constitui um tratamento promissor na reparação de lesões devido aos efeitos benéficos das plaquetas, dos fatores de crescimento e de outras moléculas biológicas nos mecanismos fisiológicos de cicatrização e regeneração tecidular. Porém, atualmente não existe consenso quanto ao valor biológico do PRP, bem como das características ideais para a sua aplicação, o que não é surpreendente dado o número de variáveis que influenciam a obtenção deste produto biológico e do potencial destas poderem interagir entre si, incluindo as variáveis do protocolo de preparação. Não obstante, o interesse no PRP tem vindo a aumentar na Medicina Veterinária, existindo, ainda, poucas referências à sua preparação em algumas espécies, como é o caso do gato. Assim, este trabalho teve como objetivo rever os princípios da terapia por PRP e otimizar um protocolo de produção de PRP em gatos, através do estudo das variáveis inerentes à sua preparação laboratorial (número de centrifugações, força e tempo de centrifugação) na eficácia de concentração de plaquetas. Adicionalmente, foram realizadas contagens de células sanguíneas de forma a caracterizar o PRP obtido. Concluiu-se que, através da metodologia usada, não foi possível concentrar plaquetas e que, dentro dos limites deste trabalho, uma única “rotação branda” (<100g, durante 5 min.) pode constituir um método relativamente simples e económico para obter PRP em gatos. De futuro, outros parâmetros que deverão ser avaliados são os efeitos isolados dos leucócitos e hemácias na preparação do PRP e mesmo a nível terapêutico, por forma a poder-se adequar a metodologia e a otimizar a obtenção das células que se pretendem aplicar na lesão.
ABSTRACT - PROTOCOL OPTIMIZATION FOR OBTAINING PLATELET RICH PLASMA IN CATS - The application of platelet-rich plasma (PRP) constitutes a promising therapeutic approach in the repair of lesions due to the beneficial effects of platelets, growth factors, and other biological molecules in the physiological mechanisms of wound healing and tissue regeneration. However, currently there is no consensus on the biological value of PRP, nor on the optimal PRP characteristics for its therapeutic application, which, given the number of variables that affect the final product characteristics and their potential to interact with each other, including variables on the preparation protocol, is not surprising. Nonetheless, interest in PRP has been increasing in veterinary medicine. There is, however, very few references on how to prepare PRP in some species, including the cat. This study aims to understand the basic principles of PRP therapy and to optimize a protocol for the production of PRP in cats, by assessing how factors derived from its preparation method (number of centrifugations, centrifugation speed and time) modify PRP concentration efficiency. In addition, blood counts were made in order to characterize the PRP. It was concluded that the methodology used in this study was unable to concentrate platelets and that, within the limits of this study, a single "soft spin" (<100g for 5 min.) could represent a relatively simple and economical method for producing PRP in cats. Future studies are necessary to assess other parameters, namely the isolated effects of leukocytes and erythrocytes on the PRP preparation and their therapeutic effects, in order to adjust the methodology and specifically obtain the cells we want to apply in the lesion.