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Готові списки джерел за темами / Plane-parallel plate

Добірка наукової літератури з теми "Plane-parallel plate"

Автор: Grafiati

Опубліковано: 30 травня 2022

Оновлено: 31 травня 2022

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Статті в журналах з теми "Plane-parallel plate"

1

Hegedus, Z. S., Z. Zelenka, and G. Gardner. "Interference patterns generated by a plane-parallel plate." Applied Optics 32, no. 13 (May 1, 1993): 2285. http://dx.doi.org/10.1364/ao.32.002285.

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2

Avendaño-Alejo, Maximino, and Martha Rosete-Aguilar. "Optical path difference in a plane-parallel uniaxial plate." Journal of the Optical Society of America A 23, no. 4 (April 1, 2006): 926. http://dx.doi.org/10.1364/josaa.23.000926.

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3

Zhong, Xing, Tiancong Wang, Junliang Yan, and Guang Jin. "Aberrations correction of tilted plane-parallel plate in convergent rays." Applied Optics 54, no. 7 (February 26, 2015): 1758. http://dx.doi.org/10.1364/ao.54.001758.

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4

Kobayashi, K., and T. Inoue. "Diffraction of a plane wave by an inclined parallel plate grating." IEEE Transactions on Antennas and Propagation 36, no. 10 (1988): 1424–34. http://dx.doi.org/10.1109/8.8630.

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5

Buyukaksoy, A., and B. Polat. "Plane wave diffraction by a thick-walled parallel-plate impedance waveguide." IEEE Transactions on Antennas and Propagation 46, no. 11 (1998): 1692–99. http://dx.doi.org/10.1109/8.736623.

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6

Kobayashi, K., and A. Sawai. "Plane Wave Diffraction by an Open-Ended Parallel Plate Waveguide Cavity." Journal of Electromagnetic Waves and Applications 6, no. 1-4 (January 1, 1992): 475–512. http://dx.doi.org/10.1163/156939392x01264.

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7

van den Eerenbeemd, Jack, and Sjoerd Stallinga. "Compact system description for systems comprising a tilted plane parallel plate." Applied Optics 46, no. 3 (January 4, 2007): 319. http://dx.doi.org/10.1364/ao.46.000319.

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8

Polat, B. "Plane wave diffraction by a semi-infinitesoft/hard parallel-plate waveguide." Canadian Journal of Physics 76, no. 10 (1998): 771–84. http://dx.doi.org/10.1139/cjp-76-10-771.

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9

Alekseeva, L. V., I. V. Povkh, M. N. Litvinova, V. I. Stroganov, and I. A. Garan’kova. "Propagation of an extraordinary beam through a plane-parallel crystal plate." Russian Physics Journal 53, no. 5 (October 2010): 539–40. http://dx.doi.org/10.1007/s11182-010-9455-5.

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10

Türetken, Bahattin, and Ali Alkumru. "Plane Wave Diffraction by an Open Parallel Plate Waveguide with Dielectric Loading." Mathematical and Computational Applications 4, no. 3 (December 1, 1999): 241–49. http://dx.doi.org/10.3390/mca4030241.

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Дисертації з теми "Plane-parallel plate"

1

Myung, Noh Hoon. "A high frequency analysis of electromagnetic plane wave scattering by perfectly-conducting semi-infinite parallel plate and rectangular waveguides with absorber coated inner walls /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487267546980859.

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2

Чорний, Владислав Олександрович. "Лазерний вимірювач швидкості кровотоку". Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2021. https://ela.kpi.ua/handle/123456789/43661.

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Обсяг звіту становить 56 сторінок, міститься 25 ілюстрацій, 17 таблиці. Загалом опрацьовано 22 джерел. Актуальність: контроль та швидка оцінка характеристик параметрів кровотоку є важливим атрибутом правильної діагностики пацієнта, а в особливості неівазивним методом вимірювання, так як сприйняття організму чужорідного тіла може вести до неприємних наслідків. Мета: недорогий та надійний лазерний вимірювач швидкості кровотока, як для лабораторних дослідів, так і для повсякденного контролю пацієнта. Завдання: 1. Огляд та аналіз літератури, що стосується лазерних вимірювачів швидкості кровотоку. 2. Огляд та аналіз інтелектуальної власності сучасних лазерних вимірювачів швидкості кровотоку. 3. Побудова оптично-функціональної схеми приладу. 4. Підбір елементів для реалізації швидкоміра. 5. Моделювання лазерного вимірювача кровотоку.
The volume of the report is 56 pages, contains 25 illustrations, 17 tables. In general, 22 sources were processed. Relevance: control and rapid assessment of the characteristics of blood flow parameters is an important attribute of proper diagnosis of the patient, and in particular a non-invasive method of measurement, as the perception of a foreign body can lead to unpleasant consequences. Purpose: inexpensive and reliable laser blood flow meter, both for laboratory experiments and for daily monitoring of the patient. Task: 1. Review and analysis of the literature related to laser blood flow meters. 2. Review and analysis of intellectual property of modern laser blood flow meters. 3. Construction of the optical-functional scheme of the device. 4. Selection of elements to the optical-functional scheme of the flowmeter. 5. Simulation of a laser blood flow meter.

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3

Oreopoulos, Lazaros. "Plane parallel albedo bias from satellite measurements." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40417.

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The plane parallel homogeneous (PPH) bias is defined as the difference between the plane parallel cloud albedo, calculated for homogeneous cloud optical depth distributions, and the independent pixel (IP) albedo, which allows for optical depth variability, but assumes that individual cloudy columns transfer solar radiation as plane parallel slabs (horizontal photon transport is neglected). Estimates of the PPH bias from extensive Advanced Very High Resolution Radiometer (AVHRR) observations are provided for areas similar in size to weather and climate model gridboxes. The goal is to investigate conditions and assumptions influencing the PPH bias, and to suggest methods to correct for it.
Visible PPH biases vary from about 0.02 to 0.30, depending on area size, view/sun geometry, and other factors influencing optical depth retrievals and albedo calculations. Broadband PPH biases are slightly smaller than visible biases; broadband absorptance biases are about an order of magnitude smaller. Approximate estimates of the bias in broadband reflected flux at cloud top often exceed 30 Wm$ sp{-2}$ for near-nadir measurements, suggesting that the assumption of cloud homogeneity produces errors that cannot be ignored in climate studies.
Solar geometry affects the PPH bias not only through the direct dependence of albedo on solar zenith angle, but also through systematic changes in the apparent mean and variance of optical depth arising from the neglect of 3-d effects in satellite radiance inversions. PPH biases decrease with data resolution, increase when atmospheric radiative effects are accounted for in optical depth retrievals, and are only slightly affected by water cloud microphysics. PPH biases are also shown to differ substantially between the forward and backward scattering directions, and between large-scale models with and without provision for fractional cloudiness.
A large fraction of the PPH bias is removable by: (1) adjusting regionally averaged optical depths with the reduction factor of Cahalan et al. (1994a), and (2) fitting observed optical depth distributions with lognormal and (to a lesser degree) gamma distributions. These methods require the logarithmic mean and variance of optical depth, which are parameterized as a function of regional mean optical depth and cloud fraction, quantities routinely available in climate models.

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4

Duan, Zhaoyang. "Parallel cutting plane algorithms for inverse mixed integer linear programming." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1468079.

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5

Lopez-Villarroya, Raul. "E-plane parallel coupled resonators for waveguide bandpass filter applications." Thesis, Heriot-Watt University, 2012. http://hdl.handle.net/10399/2604.

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High skirt selectivity and extended out-of-band rejection is a major challenge for the successful progress of in-line microwave filters. This thesis presents novel filter realizations with improved performance, compatible with the standard single thin all-metal insert in a split-block housing and therefore maintaining the low-cost fabrication characteristics. In addition, significant filter performance improvement is achieved. The synthesis procedure implemented for the filter concept consists of a few steps. Some preliminary steps are a rigorous characterization of a double-ridge coaxial waveguide, and the modelling of an equivalent circuit model for the parallel coupled ridge waveguide devised in the filter concept. From these elements, a full wave electromagnetic analysis shows that parallel-coupled asymmetric ridge waveguides produce strongly dispersive coupling which introduces a transmission zero. Later on this property is extended to parallel-coupled asymmetric ridge waveguide resonators, where it is demonstrated that it is possible to independently control the coupling coefficient and the frequency of the transmission zero. This allows the realization of pseudo-elliptic narrowband in-line bandpass filters in E-plane technology. A general synthesis procedure for high order filters is outlined and numerical and experimental results are presented for validation. The elements employed for the synthesis procedure of the bandpass prototypes are also applied to investigate structures suitable for different applications. In particular, stopband and dual stopband filters are presented with numerical and experimental results. Finally, the study of a microwave chemical/biochemical sensing device for the characterization and detection of cells in chemical substances and cells in solution in micro-litre volumes is also reported.

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6

Stewart, Kristin J. "Accurate radiation dosimetry using liquid- or air-filled plane-parallel ionization chambers." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29576.

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To optimize delivery of radiation therapy treatments, accurate knowledge of absorbed dose in the clinical beam is essential. In this work we investigated issues related to the use of different types of ionization chambers for accurate radiation dosimetry. We determined values of Pwall at 60Co for plane-parallel chambers and used them to derive the conversion factors kecal k'R 50 or kQ for a 20 MeV electron beam. Our results agreed within 0.4% with kQ values given in the IAEA TRS-398 protocol, but differences of up to 1.8% were found between our kecalk' R50 values and those in the AAPM TG-51 protocol. We also investigated the behavior of two liquid-filled ionization chambers in megavoltage photon beams, examining stability, ion recombination and beam quality dependence. Methods for determining and correcting for recombination were investigated. Preliminary results show that the energy dependence of a liquid-filled chamber can typically be limited to less than 1% for megavoltage photon beams.

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7

Nakaharai, Shu. "Vortex States under Magnetic Fields Nearly Parallel to the Plane of Layered Superconductors." 京都大学 (Kyoto University), 2001. http://hdl.handle.net/2433/150378.

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8

Min, An. "The combined effect of joints and bedding plane parallel discontinuities on rock mass deformation." Thesis, Heriot-Watt University, 1999. http://hdl.handle.net/10399/601.

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9

YOUSIF, HASHIM AZEZ. "SCATTERING OF ELECTROMAGNETIC PLANE WAVES FROM TWO INFINITELY LONG PARALLEL CYLINDERS OF ARBITRARY MATERIALS AT OBLIQUE INCIDENCE." Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184122.

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The Mueller scattering matrix elements (S(ij)) and the cross-sections for the scattering of an electromagnetic plane wave from two infinitely long, parallel, circular cylinders at oblique incidence are derived. Each cylinder can be of arbitrary materials (any refractive index). The incident wave can be in any polarization state. To find the scattering coefficients, which are essential for calculating S(ij) and the cross-sections, the multiple scatterings were taken into account for all orders such that the 'incident' field of one cylinder is the true incident field plus the scattered field from the other cylinder. The formal solutions of the scalar wave equation are obtained in the three regions; the region outside the two cylinders, the region inside each cylinder and the scattering coefficients are found by satisfying the boundary conditions. A computer program was written. The scattering coefficients are calculated from a matrix equation. This is the first comprehensive study of the two cylinder problem. Special cases are considered in comparison with other published works. Calculations are shown of S(ij) and of cross-sections for some selected cases of configurations of the two cylinders.

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10

Zakikhani, Ramtin. "Calculated perturbation factors for the NACP-02 plane-parallel ionization chamber irradiated in water by megavoltage electron beams." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=99213.

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Measurements of absorbed dose to water in megavoltage electron beams are carried out with cylindrical or plane-parallel ionization chambers. The perturbation factor associated with plane-parallel ionization chambers that have sufficiently large guard rings is assumed to be unity. The NACP-02 chamber is one such chamber that we have investigated in order to determine if this assumption is valid. By performing Monte Carlo simulations with the EGSnrc code, water-to-air Spencer-Attix stopping powers and perturbation factors were calculated in water with a detailed model of the NACP chamber that was validated through results obtained from measurements and simulations. These were determined for electron beam energies between 4 MeV and 19 MeV for a clinical and PSDL linear accelerator. The overall perturbation factor was found to decrease with energy at dref from 1.015 at 4 MeV to 1.004 at 19 MeV and increase with depth for a fixed beam quality.

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Книги з теми "Plane-parallel plate"

1

Andreo, P. Tables of charge and energy deposition distributions in elemental materials irradiated by plane-parallel electron beams with energies between 0.1 and 100 MeV. Osaka, Japan: Research Institute for Advanced Science and Technology, University of Osaka Prefecture (1-2 Gakuen-cho, Sakai, Osaka 593, Japan), 1992.

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2

H, Pathak Prabhakar, and Lewis Research Center, eds. A high frequency analysis of electromagnetic plane wave scattering by perfectly-conducting semi-infinite parallel plate and rectangular waveguides with absorber coated inner walls. Cleveland, Ohio: National Aeronautics and Space Administration, Lewis Research Center, 1986.

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3

1937-, Kattawar George W., ed. Selected papers on multiple scattering in plane parallel atmospheres and oceans: Methods. Bellingham, Wash: SPIE--The International Society for Optical Engineering, 1991.

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4

Qualey, Douglas L. Radiation from an infinite plane to parallel rows of infinitely long tubes - hottel extended. 1994.

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5

Maniatis, Lydia M. The Bathtub Illusion. Oxford University Press, 2017. http://dx.doi.org/10.1093/acprof:oso/9780199794607.003.0024.

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As we move around a picture, the light pattern projecting from it to our eye changes. The resulting percept also changes, but the nature of these changes varies from picture to picture. The contents of the picture may appear to remain parallel to the picture plane as it slants away from us, or they may undergo changes in their apparent shape and/or their orientation relative to the picture plane. The changes are a function of the geometry of the retinal projection competing with parallax cues to flatness. Here, a bathtub in a photo undergoes a radical shape change—from long and skinny to short and stubby—as we change our viewpoint.

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6

Zeitlin, Vladimir. Primitive Equations Model. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198804338.003.0002.

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The chapter gives the foundations of modelling of large-scale atmospheric and oceanic motions and presents the ‘primitive equations’ (PE) model. After a concise reminder on general fluid mechanics, the main hypotheses leading to the PE model are explained, together with the tangent-plane (so-called f and beta plane) approximations, and ‘traditional’ approximation to the hydrodynamical equations on the rotating sphere. PE are derived in parallel for the ocean and for the atmosphere. It is then shown that, with a judicious choice of the vertical coordinate, the ‘pseudo-height’, in the atmosphere, these two sets of equations are practically equivalent. The main properties of PE are derived and the key concepts of wave–vortex dichotomy, and of slow and fast motions, are explained. The essential notion of potential vorticity is introduced and its conservation by fluid masses is demonstrated. Inertia–gravity waves are explained and their properties presented. Limitations of the hydrostatic hypothesis are demonstrated.

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7

Mey, Jacob L. The Sociological Foundations of Pragmatics. Edited by Yan Huang. Oxford University Press, 2015. http://dx.doi.org/10.1093/oxfordhb/9780199697960.013.23.

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This article shows how a purely descriptive view of language cannot account for what happens to the speaking individuals on the social plane. Approaches inspired by the sociology of language and by sociolinguistics have, each from their own perspectives, endeavoured to introduce an interpretive take on language use and language users. In parallel to this, but not always coterminous with it, developments in linguistics itself have led to the establishment of what has become known as ‘pragmatics’, or the study of human communicative means (especially language), as they are being used in the context of society. Some practical applications of this view are discussed, and the emancipatory potentials of a pragmatically oriented sociology and sociolinguistics are outlined.

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8

The Use of plane parallel ionization chambers in high energy electron and photon beams: An international code of practice for dosimetry. Vienna: International Atomic Energy Agency, 1997.

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9

Zeitlin, Vladimir. Instabilities of Jets and Fronts and their Nonlinear Evolution. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198804338.003.0010.

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Notions of linear and nonlinear hydrodynamic (in)stability are explained and criteria of instability of plane-parallel flows are presented. Instabilities of jets are investigated by direct pseudospectral collocation method in various flow configurations, starting from the classical barotropic and baroclinic instabilities. Characteristic features of instabilities are displayed, as well as typical patterns of their nonlinear saturation. It is shown that in the Phillips model of Chapter 5, new ageostrophic Rossby–Kelvin and shear instabilities appear at finite Rossby numbers. These instabilities are interpreted in terms of resonances among waves counter-propagating in the flow. It is demonstrated that the classical inertial instability is a specific case of ageostrophic baroclinic instability. At the equator it appears also in the barotropic configuration, and is related to resonances of Yanai waves. The nature of the inertial instability in terms of trapped modes is established. A variety of instabilities of density fronts is displayed.

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10

International Atomic Energy Agency; IAEA. The Use of Plane Parallel Ionization Chambers in High Energy Electron and Photon Beams: An International Code of Practice for Dosimetry (Technical Reports Series (International Atomic Energy Agency)). International Atomic Energy Agency, 1997.

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Частини книг з теми "Plane-parallel plate"

1

Hovenier, Joop W., Cornelis Van Der Mee, and Helmut Domke. "Plane-parallel Media." In Astrophysics and Space Science Library, 63–95. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-2856-4_3.

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2

Natsuyama, Harriet H., Sueo Ueno, and Alan P. Wang. "Inhomogeneous Plane-Parallel Atmospheres." In Terrestrial Radiative Transfer, 19–71. Tokyo: Springer Japan, 1998. http://dx.doi.org/10.1007/978-4-431-68527-2_2.

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3

Gudehus, Gerd. "Plane-parallel evolutions without SSI." In Physical Soil Mechanics, 485–537. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-36354-5_12.

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4

Gudehus, Gerd. "Plane-parallel evolutions with SSI." In Physical Soil Mechanics, 539–635. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-36354-5_13.

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5

Boiko, Andrey V., Alexander V. Dovgal, Genrih R. Grek, and Victor V. Kozlov. "Instability of plane parallel flows." In Physics of Transitional Shear Flows, 35–46. Dordrecht: Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-2498-3_3.

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6

Lai, J. C. S., and A. Nasr. "Weakly Interacting Two Parallel Plane Jets." In Laser Techniques Applied to Fluid Mechanics, 223–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-56963-0_15.

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7

Fleischer, Matthias, Tobias Gnausch, Daniel Supp, and Jonathan Becker. "High Precision Measurement of plane-parallel Parts." In Fringe 2009, 1–4. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-03051-2_116.

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8

Gool, Luc, Theo Moons, Marc Diest, and Eric Pauwels. "Matching perspective views of parallel plane structures." In Applications of Invariance in Computer Vision, 165–84. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/3-540-58240-1_9.

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9

Dosaev, Marat, Vitaly Samsonov, and Andrei Holub. "Plane-Parallel Motion of a Friction-Powered Robot Moving Along a Rough Horizontal Plane." In Advances in Mechanism and Machine Science, 2559–65. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-20131-9_253.

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10

Inselberg, Alfred. "♣ FT-1 The Plane with Parallel Coordinates." In Parallel Coordinates, 49–61. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-68628-8_3.

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Тези доповідей конференцій з теми "Plane-parallel plate"

1

Avendano-Alejo, M., M. J. Orozco-Arellanes, and Orestes N. Stavroudis. "Ray tracing in a plane-parallel uniaxial plate." In SPIE Proceedings, edited by Aristides Marcano O. and Jose Luis Paz. SPIE, 2004. http://dx.doi.org/10.1117/12.592193.

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2

Shirai, H., and K. Watanabe. "Plane wave scattering by staggered parallel plate waveguide cavities." In Proceedings of ISAPE 2000: Fifth International Symposium on Antennas, Propagation, and EM Theory. IEEE, 2000. http://dx.doi.org/10.1109/isape.2000.894821.

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3

Avendanyo-Alejo, Maximino M., and Martha Rosete-Aguilar. "Optical path difference in a plane parallel uniaxial plate." In Merida - DL Tentative, edited by R. Rodriguez-Vera and F. Mendoza-Santoyo. SPIE, 2005. http://dx.doi.org/10.1117/12.611648.

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4

Scheibengraber, Karl J. "Plane-parallel plate method of laser localization and laser positioning." In OE/LASE '94, edited by Leonard J. Cerullo, Kenneth S. Heiferman, Hong Liu, Halina Podbielska, Abund O. Wist, and Lucia J. Zamorano. SPIE, 1994. http://dx.doi.org/10.1117/12.176598.

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5

Ciosek, Jerzy. "Interference coatings on the plane-parallel plate in laser applications." In Tenth Polish-Czech-Slovak Optical Conference: Wave and Quantum Aspects of Contemporary Optics. SPIE, 1998. http://dx.doi.org/10.1117/12.301346.

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6

Lee, C., J. E. Park, H. Choi, J. Kim, M. Cha, and J. Jin. "Optical interferometry with transmitted Haidinger fringes through a plane parallel plate." In Conference on Lasers and Electro-Optics/Pacific Rim. Washington, D.C.: OSA, 2018. http://dx.doi.org/10.1364/cleopr.2018.tu3f.6.

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7

Bogatyryova, G. V., and D. Y. Kondratenko. "Analysis of influence of plane-parallel plate on resolving power of Fourier spectrometer." In Correlation Optics 2011, edited by Oleg V. Angelsky. SPIE, 2011. http://dx.doi.org/10.1117/12.920575.

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8

Bernacki, Bruce E., Kannan Krishnaswami, and Norman C. Anheier. "Low cost plane parallel plate lateral shearing interferometer for infrared laser beam diagnostics." In SPIE Defense, Security, and Sensing, edited by Mark Dubinskii and Stephen G. Post. SPIE, 2009. http://dx.doi.org/10.1117/12.817416.

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9

Bodrov, S. B., N. A. Abramovsky, E. A. Burova, A. N. Stepanov, and M. I. Bakunov. "Tilted-Pulse-Front Terahertz Generation in a Plane-Parallel LiNbO₃ Plate." In 2021 46th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz). IEEE, 2021. http://dx.doi.org/10.1109/irmmw-thz50926.2021.9567046.

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10

Zhang, Min, Lei Zhao, Lijian Dong, Yongxin Sui, and Huaijiang Yang. "A deformable plane-parallel optical plate with 16 actuated points for low order aberrations correction." In Eighth International Symposium on Advanced Optical Manufacturing and Testing Technology (AOMATT2016), edited by Yadong Jiang, Bernard Kippelen, and Junsheng Yu. SPIE, 2016. http://dx.doi.org/10.1117/12.2241405.

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Звіти організацій з теми "Plane-parallel plate"

1

Gautier, C. Solar radiation absorption in the atmosphere due to water and ice clouds: Sensitivity experiments with plane-parallel clouds. Office of Scientific and Technical Information (OSTI), September 1995. http://dx.doi.org/10.2172/232593.

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2

Boisclair, Yves R., Alan W. Bell, and Avi Shamay. Regulation and Action of Leptin in Pregnant and Lactating Dairy Cows. United States Department of Agriculture, July 2000. http://dx.doi.org/10.32747/2000.7586465.bard.

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Анотація:

The original project had four specific objectives: (1) To complete the development of a radioimmunoassay for bovine leptin; (2) To characterize the leptin system in lactating dairy cows during the transition from pregnancy to lactation; (3) To identify endocrine factors regulating the production of leptin by bovine adipose tissue; (4) To study the actions of leptin on bovine adipose and mammary tissues in vitro. However, BARD funded only the development of the bovine leptin RIA (Objective 1) for a single year. This report describes our work in completing this objective. Leptin, a protein hormone secreted predominantly by white adipose tissue, plays a critical role in the regulation of energy metabolism. In rodents and humans, leptin informs the central nervous system of the size of the energy reserves, coordinates adaptations to periods of nutrient insufficiency, and regulates the metabolism of key tissues involved in the storage and dissipation of energy. However, almost nothing is known on the biology of leptin in cattle, in part because of the absence of a valid assay to measure bovine leptin. To remediate this situation, we have developed a radioimmunoassay capable of measuring bovine leptin with a high degree of sensitivity, accuracy and precision. First, we produced recombinant bovine leptin and used it to immunize rabbits, and to prepare bovine leptin trace and standards. A single antiserum with sufficient affinity and titer was identified. Using this antiserum, binding of 125I bovine leptin was displaced in a dose dependent manner by the addition of bovine or ovine leptin. Serial dilution of bovine and ovine plasma gave displacement curves that were parallel to that of bovine or ovine leptin. Recoveries of external addition of bovine leptin in ewe and cow plasma ranged between 94 and 104%. Plasma leptin concentration measured by this assay was increased by the plane of nutrition in growing calves and lambs. Finally, plasma leptin concentration was linearly related to the fat content of the empty carcass in growing cattle. We conclude that circulating leptin in sheep and cattle is increased by fatness and plane of nutrition, consistent with results in humans and rodents. This assay provides an important tool to investigate mechanisms that regulate plasma leptin in cattle and sheep.

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