Дисертації з теми "Phytase Production"
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Kerovuo, Janne. "A novel phytase from Bacillus : characterization and production of the enzyme." Helsinki : University of Helsinki, 2000. http://ethesis.helsinki.fi/julkaisut/mat/bioti/vk/kerovuo/.
Повний текст джерелаMoss, Amy F. "Nutritional strategies to enhance the efficiency of chicken-meat production." Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/20031.
Повний текст джерелаSpier, Michele Rigon, Adenise Lorenci Woiciechowski, Carlos Ricardo 1953 Soccol, and Universidade Federal do Paraná Setor de Tecnologia Programa de Pós-Graduaçao em Processos Biotecnológicos. "Development of a bioprocess for production of a new A. niger FS3 Phytase." reponame:Repositório Institucional da UFPR, 2009. http://hdl.handle.net/1884/18314.
Повний текст джерелаTang, Shuiquan. "Process engineering of Pichia pastoris cultivation for the production of a phytase with GAP promoter." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28277.
Повний текст джерелаZhong, Shuping. "Study of Operational Strategies and Carbon Source Selection for the Production of Phytase using Pichia pastoris." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32204.
Повний текст джерелаEbune, Anne Ebane. "Production of phytase and reduction of phytic acid content in canola meal by solid state fermentation." Thesis, University of Ottawa (Canada), 1992. http://hdl.handle.net/10393/10855.
Повний текст джерелаAl-Asheh, Sameer. "Production of phytase and reduction of phytic acid content in canola meal by solid state fermentation using Aspergillus carbonarius." Thesis, University of Ottawa (Canada), 1993. http://hdl.handle.net/10393/7578.
Повний текст джерелаContreras, Tobar Edith Andrea. "Evaluación productiva de una fitasa de origen microbiano (Ronozyme ® Phytase) en dietas de pollos Broiler." Tesis, Universidad de Chile, 2001. http://repositorio.uchile.cl/handle/2250/132085.
Повний текст джерелаSe investigó el efecto de la incorporación de una fitasa comercial de origen microbiano (Ronozyme® Phytase) en dietas de pollos broiler. Se evaluó el comportamiento productivo de las aves mediante un experimento de 43 días de duración en que se utilizaron seis mil pollos broiler Ross 308 de 1 día de edad, 50% machos y 50% hembras. El experimento consistió en 4 tratamientos: 1) Dieta control sin adición de fitasa; 2) Dieta control mas fitasa (750 FTU de fitasa/kg de dieta) en reemplazo de un 0,1% del fósforo total proveniente del fosfato de calcio; 3) Dieta formulada con los mismos ingredientes de la dieta control con la adición de 750 FTU de fitasa/kg de dieta, considerando un aporte nutritivo de la fitasa de 13 kcal/kg de EMAn, 0,35% de proteína, 0,013% de lisina, 0,009% de metionina+cistina, 0,1% de Ca y 0,1% de P total, valores que se descontaron del aporte nutritivo total de la dieta; 4) Dieta idéntica al tratamiento 3, descontados los valores nutricionales recién señalados, pero sin adición de fitasa. A lo largo del experimento se utilizaron 3 dietas para cada tratamiento según los siguientes períodos: inicial (1 a 21 días); intermedia (22 a 38 días) y final (39 a 43 días). Todas las dietas fueron formuladas a base de maíz, afrecho de soya, gluten de maíz, harina de pescado y aceite vegetal. Los indicadores productivos controlados fueron peso vivo los días 1, 21 y 43, consumo de alimento, eficiencia de conversión alimenticia (ECA) y mortalidad a los 21 y 43 días de edad. Además, se calculó el porcentaje de cenizas en falanges de una muestra de 120 pollos por cada tratamiento al finalizar el período experimental. Adicionalmente, se registró el número de pollos que presentaron anormalidades esqueléticas que se manifestaron con un desplazamiento anormal de las aves a los 21 y 43 días de edad. La adición de fitasa en dietas de pollos broiler reemplazó el aporte parcial de fósforo (P) inorgánico, aminoácidos, proteína, Ca y energía metabolizable aparente corregida para nitrógeno (EMAn), sin afectar los indicadores productivos e integridad ósea de las aves. La reducción de EMAn, proteína, lisina, metionina+cistina, Ca y P de la dieta en ausencia de fitasa influyó negativamente sobre el rendimiento productivo de las aves de 1 a 43 días de edad. El cálculo económico realizado indica que la adición de 750 FTU de fitasa mejora la utilidad por pollo cuando la enzima sólo reemplaza 0,1% de P disponible de la dieta
Misrahi, Audrey. "Transformation de végétaux par fermentation en milieu solide pour la production d'outils enzymatiques et de biomasse valorisée : application au couple "grain de maïs/Fusarium venenatum".Invertigations pour la production de phytases et de polysaccharidases." Université Louis Pasteur (Strasbourg) (1971-2008), 2007. http://www.theses.fr/2007STR13247.
Повний текст джерелаWe developed a solid state fermentation process. It focuses on the growth of the fungus Fusarium venenatum on a substrate based on whole maize kernel. Designs of solid cultures have been used at the laboratory scale, to define the process and, further, assist its optimization. A specific solution of following the fungus growth has been found, which use antibody anti-Fusarium. F. Venenatum/maize kernel has been tested for its ability to produce polysaccharidases and phytases. This couple leads to the production of phosphatases with a probable large range of substrates, including phytic acid, but not to “strict” phytases. Its potential for the production of polysaccharidases has been confirmed, through enzymatic assay measurements and mass spectrometry experiments. Those ones used data on F. Graminearum’s proteins. Results also indicate other final products, as well as the production of a liquid extract rich in glucose, fungi proteins and inorganic phosphate
Wu, Pei-Hua, and 吳佩樺. "Production of Escherichia coli phytase by recombinant Pichia pastoris." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/38609423050340225335.
Повний текст джерела國立臺灣大學
農業化學研究所
88
Phytase, a valued feed additive for monogastric animals, has been used to hydrolyze phytic acid in feeds and to reduce the antinutrient ability of phytic acid. It also helps the release of phosphorous from phytic acid to increase phosphorous digestibility. In this study, the production of Escherichia coli phytase in recombinant Pichia pastoris KM 71-61 was investigated and the biochemical characteristics of said phytase were presented. By adding 0.5% methanol every 24 h during the stationary phase in the flask cultures, the phytase activity reached up to 150 U/ml after 144 h of induction. The phytase activity increased one fold in the case of replacing culture with fresh medium before induction. With the same strategy, the phytase activity is about 600 U/ml in fermentor cultures after 168 h of induction. The increase of phytase activity is not resulted from adding extra nutrients but from removing inhibitors. The phytase activity on cellular base was higher when initiated with lower cell density. The starvation before induction has little effect on the phytase activity due to the slow utilization of methanol in Pichia pastoris KM 71- 61.
Chen, Yu, and 陳褕. "Purification and immobilization phytase for the production of inositoll." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/18804418671812097792.
Повний текст джерела國立東華大學
生物技術研究所
87
Phytase (myo-inositol hexakisphosphate phosphohydrolase) hydrolyzes phytic acid to myo-inositol and phosphoric acid. Two types of phytase are known: 3-phytase (E.C.3.1.3.8) and 6-phytase (E.C. 3.1.3.26) by indicating the phosphoester bond position of the first attack. It was reported that phytase activity was assayed by measuring the rate of increase in inorganic orthophosphate (pi) and the extracted phosphomolybdate. The method was used with the colorimetric measurements of the activity of phosphomolybdate . In this study, We used the concentration of inositol released from phytic acid to indicate the activity of this enzyme by monitoring the rate of inositol production using HPLC-RI detector. Phytases were prepared from E. coli K12 . The purification steps include cell culture, sonication, ammonium sulfate precipitation, Ion-exchange, and Gel-filtration chromatography. The final purity of phytase was 79.3 folds , and the maxium activity of the enzyme was 102.3 U/mg , when the enzyme reaction was under the condition:pH 4.5 and 55oC . The kinetic investigations showed that Vmax = 184μM/min; Km= 10.8 mM; Kcat = 200 min-1 at the optimum enzyme reactive conditions of pH 4.5, at 55 oC The tolerance limit for temperature was 55 oC. The production rate of inositol from phytic acid was 72.2 %. The immobilization of phytase was accomplished by using chitosan beads. The optimun enzyme absorbed was 0.38 mg / g bead . For the optimum reactive condition of immobilized enzyme was pH 4.5 and 60 oC; howevere, the enzyme activity had only 25.2 % of the free enzyme activity (Kcat) and the production rate of inositol from phytic acid was 25.5 %.
Tseng, Shiann-Ming, and 曾憲明. "Production and Charaterization of Extracellular Phytase from Aspergillus ficuum mutant." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/52652009923489204568.
Повний текст джерела國立中興大學
食品科學研究所
83
Eighty three microorganisms containing phytase activity were isolated from different sources of soil. Strain M46 was found to have the highest enzyme activity among these organisms. For further improvement, Aspergillus ficuum NRRL 3135 and strain M46 were treated with the mutagenic agent NTG (N-methyl-N-nitro-N'-nitrosoguanidine) and plated onto PSM agar containing calcium phytate as a selective medium. One of the NTG-treated strains, A. ficuum mutant A200413, showed considerably higher phytase activity than the parent strain A. ficuum NRRL 3135 and was chosen for further investigation. This mutant strain A200413 was cultivated in shaking flasks and the optimal medium for the phytase production by this strain were as follows: molasses, 12.93%; ammonium nitrate, 0.5%; KCl; 0.05%; MgSO4.7H2O, 0.05%; KH2PO4, 0.002%; FeSO4. 7H2O, 0.001%; and MnSO4.4H2O, 0.001%. For cuitivations, initial pH at 4.0, inoculum size at 10(7) spores/50 ml, incubation temperature at 35℃, and shaking rate at 150 rpm were found to be the optimal conditions. The maximal phytase activity of 5633.34 U/ml was obtained after 6 days of incubation under the optional conditions. The optimal temperature and pH for the crude ezyme activity of mutant A200413 were 58-60℃ and 5.5, respectively. This enzyme was stable at pH 2.0 and pH 4.0 and maintained 90% of its activity after incubated at 55℃ for 10 min. After 60 days of storage at -20℃, only 20% loss of enzyme activity was found. The activity of the crude phytase was found to be inhibited by Ag+, Cu2+, Fe3+, Mn2+, Na+, Zn2+ and oxalate, whereas the enzyme activity was increased by the presence of Mg2+, citrate and EDTA. Ca2+ and K+ had no effect on the catalytic rate of the crude phytase from strain A200413.
Hsieh, Meng Chou, and 謝孟洲. "Estimation of simultaneous production for xylanase/phytase and optimal production of xylanase from Aspergillus carneus M34." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/46760344500471002172.
Повний текст джерела國立中興大學
食品科學系
92
The objective of this study was to evaluate the possibility of enhancement of xylanase and phytase activity simultaneously from Aspergillus carneus M34 and to establish the optimal conditions for xylanase production by using experimental design methodology. The effects of nutrition on the enzyme activity were evaluated, and then the important factors were selected through fractional factorial design. The steepest ascent design was used to establish the superior conditions for xylanase production. Effects of the phosphorous source, culture temperature, and initial pH on the xylanase activity were evaluated as well. The optimal enzyme activity was obtained by central composite design. The results from the phosphorous source, carbon source, and experiment design studies showed that both of xylanase and phytase activity could not be improved simultaneously by selecting the nutritional factors and their concentrations. Xylanase activity was estimated to be 11.66 U/ ml by the mathematic model of central composite design, and the stationary point was found to be a saddle point. The optimal conditions for the xylanase activity from A. carneus M34 are as follows: 1.5% hemicellulose (from coba husk), 0.8 % yeast extract, 0.4% ammonium chloride, initial pH at 7.00, incubation temperature at 30℃. After 5 days of cultivation, the maximal enzyme activity was 27.31±1.14 U/ ml.
Chiang, Chen-Hua, and 蔣正華. "Studies on the production of inositol from phytic acid using immobilized phytase." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/64096360752894236709.
Повний текст джерелаChanderman, Ashira. "Production, characterisation and applications of a thermo-acid-stable phytase from Enterobacter sp. ACSS." Thesis, 2016. http://hdl.handle.net/10321/1745.
Повний текст джерелаA bacterial strain producing an extracellular phytase was identified as Enterobacter sp. ACSS. Optimization of process parameters using statistical methods such as Plackett-Burman design (PBD), the steepest ascent method, and response surface methodology (RSM) significantly improved phytase production by 4.6–fold in shake-flasks. In addition, an overall 1.9-fold increase in phytase production was attained in fed-batch fermentations in a 5 l laboratory fermenter, respectively. The purified 62 kDa phytase from Enterobacter sp. ACSS was active between 40 to 80°C and an acidic pH range of 2.0 to 6.0 with half-life of 693 and 577.5 min at 60°C and pH 2.0, respectively. Additionally, the enzyme is fairly stable with proteolytic enzymes under physiological conditions. It was activated by Ca+2, Mg+2 and Mn+2 while inhibition was caused by Zn+2, Cu+2, Fe+2, Pb+2, Co+2, Ba+2 and surfactants. The Km, Vmax and Kcat observed were 0.21 mM, 131.58 nmol mg-1s-1 and 1.64 × 103 s-1, respectively. The enzyme released inorganic phosphate from animal feed (4.0-6.62 mg/g of diet) and insoluble metal-phytates (45-219 µg/ml) and was effective in improving the characteristics of brown bread. Overall, this study shows that Enterobacter sp. ACSS has the potential to produce significant titres of a thermo- and acid-stable phytase and can be applied in dephytinizing animal feeds, and the baking industry.
M
Shieh, I.-Chuan, and 謝衣鵑. "Studies on the production, partial purification and characterization of the phytase from penicillium simplicissimum W46." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/72175918046161592751.
Повний текст джерелаHong, Ya-Fang, and 洪雅芳. "Transgenic Potato and Sweet Potato for Production of High Efficiency Phytase for Use as Animal Feed Additive." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/07464458711963290932.
Повний текст джерелаBenchabane, Samir. "Production de microcapsules de phytase par atomisation: influence sur la disponibilité des nutriments chez la truite arc-en-ciel (Oncorhynchus mykiss) /." 2005. http://proquest.umi.com/pqdweb?did=1075706801&sid=3&Fmt=2&clientId=9268&RQT=309&VName=PQD.
Повний текст джерела