Добірка наукової літератури з теми "Phomopsis"

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Статті в журналах з теми "Phomopsis"

1

Croker, KP, and MA Johns. "Reproductive performance of ewes fed sweet narrow-leafed lupin seed infected with Phomopsis leptostromiformis." Australian Journal of Experimental Agriculture 25, no. 3 (1985): 536. http://dx.doi.org/10.1071/ea9850536.

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An experiment was designed to examine the reproductive performance of Merino ewes fed sweet narrow-leafed lupin seed infected with Phomopsis leptostromiformis and containing small quantities of the toxin phomopsin. Two groups of mature ewes were supplemented with lupin seed with either a low (2%) or a high (21%) level of Phomopsis infection at a rate equivalent to 500 g/sheep.day for a period from 14 days before the start of joining until day 17 of joining. A third group received no supplement. The percentages of lambing ewes with multiple births were marginally increased (P<0.10) in the supplemented ewes (14.3, 27.5 and 26.2% for the unsupplemented and the 2 and 21% Phomopsis infected seed groups, respectively) while the percentages of lambs born were 99.0, 93.6 and 112.8% for the unsupplemented and the 2 and 21 % Phomopsis-infected seed groups, respectively. Supplementation of ewes with lupin seed containing a relatively high level of Phomopsis (21%), but a low quantity of phomopsin, did not depress reproductive performance.
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2

Książkiewicz, M., K. Wójcik, W. Irzykowski, W. Bielski, S. Rychel, J. Kaczmarek, P. Plewiński, E. Rudy, and M. Jędryczka. "Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection." Journal of Applied Genetics 61, no. 1 (October 22, 2019): 1–12. http://dx.doi.org/10.1007/s13353-019-00521-y.

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Abstract The fungus, Diaporthe toxica, anamorph Phomopsis sp., previously classified as P. leptostromiformis, is a plant endophyte and occasional pathogen, causing Phomopsis stem blight. This disease is damaging not only to lupins but also to the animals grazing on infected plants, due to the toxic secondary metabolites called phomopsins. The aim of this work was to validate markers for resistance to Phomopsis stem blight in narrow-leafed lupins and identify novel germplasm with increased levels of resistance to the disease. Plant inoculations were performed using ten isolates of D. toxica, originating from Australia and Poland. The European core collection of L. angustifolius was evaluated both in a controlled environment and with field experiments to classify the accessions based on their resistance to the disease. Simultaneously, the accessions were assayed with disease resistance markers to identify donors of hypothetical resistance alleles. We have found that the European lupin germplasm collection preserves wild and domesticated donors of at least two resistance genes to Phomopsis stem blight, including Phr1 and PhtjR. Molecular markers PhtjM7, InDel2, and InDel10, tagging PhtjR gene, were applicable for marker-assisted selection targeting the European gene pool with an expected accuracy of 95%. None of diagnostic markers for the Phr1 locus was found useful for European breeding programs; two existing markers Ph258M1 and Ph258M2 were unreliable, due to a high percentage of false-positive results (up to 58%) and a high recombination rate between markers (~ 30%).
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3

Wood, PM, DS Petterson, GR Hancock, and GA Brown. "Distribution of seed infected with Phomopsis leptostromiformis and of phomopsin A within a lupin crop." Australian Journal of Experimental Agriculture 27, no. 1 (1987): 77. http://dx.doi.org/10.1071/ea9870077.

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A detailed study was made of the Phomopsis leptostromiformis infection and phomopsin A content of seed within a lupin crop having a high level of visible pod infection. Over 96% of the visibly infected seeds contained viable P. leptostromlformis. Visually clean, or whole white seed, from the primary pods had a higher level of fungal infection (56%) than comparable seed from the other pods (11%). The discoloured seed content of the primary pods was higher than for the other pods in each instance. The phomopsin A content of the seed was higher than previously reported. Although the cracked and discoloured seed fractions contained from 200 to 2300 �g of phomopsin A/kg, the highest amount of this toxin detected in whole white seed was 6 �g/kg.
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4

Tuset, J. J., and M. A. T. Portilla. "Taxonomic status of Fusicoccum amygdali and Phomopsis amygdalina." Canadian Journal of Botany 67, no. 5 (May 1, 1989): 1275–80. http://dx.doi.org/10.1139/b89-168.

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A comparative study was done in Spain of canker and withering of branches in almond and peach trees. Similar diseases of these fruit trees, described by several authors and attributed to Fusicoccum amygdali and Phomopsis amygdalina, suggests that these fungi are identical. Spanish isolates produced phialidic conidiogenous cells and occasionally β-conidia typical of the genus Phomopsis. A study of the type specimen of F. amygdali reveals that it also belongs in Phomopsis. Phomopsis amygdali (Del.) Tuset & Portilla comb.nov. is proposed to accommodate the fungus; Phomopsis amygdalina Canonaco is reduced to synonymy with it.
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5

Petterson, DS, JE Peterson, LW Smith, PM Wood, and CCJ Culvenor. "Bioassay of the contamination of lupin seed by the mycotoxin phomopsin." Australian Journal of Experimental Agriculture 25, no. 2 (1985): 434. http://dx.doi.org/10.1071/ea9850434.

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Samples of seed from commercial crops of Lupinus spp. in three States were tested for the presence of phomopsin, the causative agent of lupinosis. Each of 43 samples was tested in one of two laboratories using a nursling rat bioassay, and 12 of these were tested in both. Factors that could affect reproducibility of the assay were examined. There was good agreement in assessments of toxicity between laboratories. The efficiency of extraction was found to vary from about 15% at low concentrations of phomopsin to no more than 60%. Phomopsin was detected in 17 of the 43 samples, at levels ranging from < 6 �g/kg to 360 �g/kg. Phomopsis leptostromiformis infection was detected in 25 of 31 samples of seed from Western Australia, the highest infection level being 18%. The highest levels of phomopsin were found in samples with more than 8% infection.
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6

Vukojević, J., M. Mihaljčević, and D. Franić-Mihajlović. "VARIABILITY OF Phomopsis POPULATIONS IN SUNFLOWER (Helianthus annuus L.) / VARIABILIDAD DE LAS POBLACIONES DE Phomopsis EN EL GIRASOL (Helianthus annuus L.) / VARIABILITÉ DES POPULATIONS DE Phomopsis CHEZ LE TOURNESOL (Helianthus annuus L.)." HELIA 24, no. 34 (July 2001): 69–76. http://dx.doi.org/10.1515/helia.2001.24.34.69.

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SUMMARY Various reports state that the sunflower disease, primarily caused by Diaporthe/Phomopsis helianthi Munt.-Cvet. et al., has a wide geographic distribution in Europe, USA, Argentina and Australia. Various Phomopsis isolates obtained during this investigation of the sunflower disease differed in conidial type (α, β, α and β). Phomopsis helianthi was the only isolate producing exclusively β-conidia and perithecia on debris (Diaporthe helianthi). The other Phomopsis isolates were saprobes on sunflower plants.
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7

Radiastuti, Nani, Firdaus Ramadhan, and Yusraini Dian Inayati Siregar3. "Antioksidan Ekstrak Kapang Endofit Phomopsis spp. dari Tanaman Kina (Cinchona calisaya)." Jurnal Biotek Medisiana Indonesia 10, no. 2 (January 18, 2022): 109–15. http://dx.doi.org/10.22435/jbmi.v10i2.5822.

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ABSTRACT Phomopsis spp. is one of the endophytic fungi that was isolated from cinchona plant (Cinchona calisaya). Endophytic fungi ability to produce bioactive compounds similar to the host, as a solution to get bioactive compounds without doing a lot of exploitation in the quinine plant. This study aimed to determine the highest antioxidant activity (IC50) and known compounds acting as antioxidants in endophytic fungi extract Phomopsis spp. The extraction process was done by the maceration method by organic solvent ethyl acetate. The measured parameters IC50 and analysis of bioactive compound in extracts of endophytic fungi Phomopsis spp. The IC50 test used 1.1-diphenyl-2-picrilhidrazil (DPPH) method with UV-Vis spectrophotometry (λ 517 nm) and identification bioactive compound with Gas Chromatography-Mass Spectroscopy (GC-MS). Based on the results of the DPPH test extract of Phomopsis spp. M70 had the highest antioxidant activity with IC50 1283.95 ppm. Results of GC-MS showed hexadecanoic acid, octadecanoic acid 2-(2-hydroxyethyl) ethyl ester, benzene ethanol, 4-hydroxy-, 9-octadecanoic acid, hexadecanoic acid 2-hydroxy-1-(hydroxymethyl) ethyl ester and octadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester compounds which served as antioxidants in the extract of Phomopsis spp. M70. Extract of Phomopsis spp. M70 had the highest IC50 value but its antioxidant ability was classified as inactive. Keywords: antioxidant, cinchona plants, endophytic fungi, Phomopsis spp. ABSTRAK Phomopsis spp. merupakan salah satu jamur endofit yang diisolasi dari tanaman kina (Cinchona calisaya). Kemampuan kapang endofit menghasilkan senyawa bioaktif yang mirip dengan inang, sebagai solusi mendapatkan senyawa bioaktif tanpa banyak melakukan eksploitasi pada tanaman kina. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan (IC50) tertinggi dan mengetahui senyawa yang berperan sebagai antioksidan dalam ekstrak jamur endofit Phomopsis spp. Proses ekstraksi dilakukan dengan metode maserasi dengan pelarut etil asetat. Parameter yang diukur IC50 dan analisis senyawa bioaktif dalam ekstrak jamur endofit Phomopsis spp. Uji IC50 menggunakan metode 1.1-difenil-2-picrilhidrazil (DPPH) dengan spektrofotometer UV-Vis (λ 517 nm) dan identifikasi senyawa bioaktif dengan Gas Chromatography-Mass Spectroscopy (GC-MS). Berdasarkan hasil uji DPPH ekstrak Phomopsis sp. M70 memiliki aktivitas antioksidan tertinggi dengan IC50 1283,95 ppm. Hasil GC-MS menunjukkan asam heksadekanoat, asam oktadekanoat 2- (2-hidroksietoksi) etil ester, benzenaetanol, 4-hidroksi-, asam 9-oktadekanoat, asam heksadekanoat 2-hidroksi-1- (hidroksi metil) etil ester dan asam oktadekanoat, 2 Senyawa -hidroksi-1- (hidroksimetil) etil ester yang berperan sebagai antioksidan pada ekstrak Phomopsis spp. M70. Ekstrak Phomopsis spp. M70 memiliki IC50 tertinggi namun kemampuan antioksidannya tergolong tidak aktif Kata kunci: antioksidan, kapang endofit, Phomopsis spp., tanaman kina
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8

Murali, T. S., T. S. Suryanarayanan, and R. Geeta. "Endophytic Phomopsis species: host range and implications for diversity estimates." Canadian Journal of Microbiology 52, no. 7 (July 1, 2006): 673–80. http://dx.doi.org/10.1139/w06-020.

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Foliar endophyte assemblages of teak trees growing in dry deciduous and moist deciduous forests of Nilgiri Biosphere Reserve were compared. A species of Phomopsis dominated the endophyte assemblages of teak, irrespective of the location of the host trees. Internal transcribed spacer sequence analysis of 11 different Phomopsis isolates (ten from teak and one from Cassia fistula) showed that they fall into two groups, which are separated by a relatively long branch that is strongly supported. The results showed that this fungus is not host restricted and that it continues to survive as a saprotroph in teak leaf, possibly by exploiting senescent leaves as well as the litter. Although the endophyte assemblage of a teak tree growing about 500 km from the forests was also dominated by a Phomopsis sp., it separated into a different group based on internal transcribed spacer sequence analysis. Our results with an endophytic Phomopsis sp. reinforce the earlier conclusions reached by others for pathogenic Phomopsis sp., i.e., that this fungus is not host specific, and the species concept of Phomopsis needs to be redefined.Key words: fungal endophytes, Tectona grandis, fungal diversity, Phomopsis, host restriction.
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9

Li, Shuxian, Glen L. Hartman, and Deborah L. Boykin. "Aggressiveness of Phomopsis longicolla and Other Phomopsis spp. on Soybean." Plant Disease 94, no. 8 (August 2010): 1035–40. http://dx.doi.org/10.1094/pdis-94-8-1035.

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Phomopsis seed decay of soybean is a major cause of poor-quality soybean seed. The disease is caused primarily by the fungal pathogen Phomopsis longicolla. Aggressiveness of isolates of P. longicolla from soybean and other Phomopsis spp. from other hosts were compared by inoculating 2-week-old soybean plants of cv. Williams 82. There were significant (P ≤ 0.0001) differences among isolates based on stem length and stem lesion length. The P. longicolla soybean isolate PL16, from Mississippi, caused the shortest stem length while the non-soybean isolate P9, from Illinois, caused the greatest stem lesion length. The type isolate of P. longicolla, PL31 (Fau 600), was one of the 3 most aggressive isolates among all 48 isolates tested. The velvetleaf isolate P9 was the most aggressive among 13 isolates from non-soybean hosts. This study provided the first evaluation of aggressiveness of P. longicolla isolates from different geographic origins and the first demonstration that Phomopsis spp. isolated from cantaloupe, eggplant, and watermelon infected soybean. Knowledge about the variability of the pathogen is important for selecting isolates for breeding soybean lines with broad-based resistance to Phomopsis seed decay.
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Wrather, J. A., D. A. Sleper, W. E. Stevens, J. G. Shannon, and R. F. Wilson. "Planting Date and Cultivar Effects on Soybean Yield, Seed Quality, and Phomopsis sp. Seed Infection." Plant Disease 87, no. 5 (May 2003): 529–32. http://dx.doi.org/10.1094/pdis.2003.87.5.529.

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Incidence of Phomopsis seed decay is frequently high and quality low in seed from early-maturing maturity group III and IV soybean cultivars planted in early to mid-April in the southern United States. Cultivars resistant to this disease have not been available until the recent release of germ plasm lines SS 93-6012 and SS 93-6181. Our objective was to determine the effects of planting dates with these lines and one Phomopsis seed decay-susceptible soybean cultivar, Asgrow 3834, on seed infection by Phomopsis spp. and on yield and the correlation between percentage of Asgrow 3834 infected with Phomopsis spp. and seed quality. Generally, yields averaged over years were significantly greater for mid-April than mid-June plantings, and yields of cultivars were similar within a planting date. Soybean lines SS 93-6012 and SS 93-6181 were highly resistant to Phomopsis seed decay compared with the susceptible cultivar, Asgrow 3834. There was a significant, negative correlation between germination of seed from mid-April plantings of Asgrow 3834 and percentage of these seed infected with Phomopsis spp. Moreover, there were significant correlations between fatty acid composition of Asgrow 3834 seed and the percentage of these seed infected with Phomopsis spp. This altered composition of fatty acids may be responsible for reduced quality of oil derived from seed infected with this fungus. Phomopsis seed decay-resistant soybean lines SS 93-6012 and SS 93-6181 should be useful in breeding programs focused on developing high-yielding cultivars resistant to this disease.
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Дисертації з теми "Phomopsis"

1

Jellal, Abdelilah. "Production, isolement et purification de deux chaetoglobosines produites par le champignon Phomopsis leptostromiformis : études structurale et toxicologique." Paris 7, 1993. http://www.theses.fr/1993PA077331.

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La culture sur mais de la souche mrc 2654 du phomopsis leptostromiformis n'a pas conduit à la production de la phomopsine. Néanmoins, le résidu méthanolique du maïs contaminé présentait une toxicité aigue vis à vis des rats. Une méthodologie d'analyse et de purification associée à un contrôle des activités biologiques des fractions purifiées nous a permis, grâce en particulier a la clhp préparative sur silice, de localiser et d'isoler 2 toxines cristallisées a4 et a4' à des taux respectifs de 80 et 40 mg par kg de maïs contaminé. L’étude structurale des 2 toxines basée sur l'analyse spectroscopique par fab/ms et par rmn 1d et 2d, montre que les deux toxines appartiennent à la famille des chaetoglobosines. La toxine a4 a pu être identifiée à la chaetoglobosine m tandis que la toxine a4' s'avère être une nouvelle chaetoglobosine. L’étude de la cytotoxicite in vitro sur hépatocytes de rats des chaetoglobosines a permis d'estimer leur ci50 respectivement à 70 et 64 ug/ml. La dl50 déterminée in vivo sur rat est d'environ 20 mg par kg de rat pour les deux toxines. La bonne corrélation des résultats obtenus in vitro et in vivo montre que les 2 chaetoglobosines sont des produits hépatotoxiques. L’étude réalisée in vitro sur cellules ovariennes d'hamster montrent que les chaetoglobosines a4 et a4' inhibent le clivage cytoplasmique des cellules. Enfin un suivi par clhp de la production en milieu liquide au cours du temps des chaetoglobosines a4 et a4' a montré que l'obscurité est un facteur favorable a leur production dont l'optimum est atteint vers 10 jours d'incubation
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2

Scheper, Reiny W. A. "Studies on the biology and genetic variation of phomopsis on grapevine /." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phs325.pdf.

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3

Miric, Elizabeth. "Pathological, morphological and molecular studies of a worldwide collection of the sunflower pathogens phomopsis helianthi and phoma macdonaldii /." St. Lucia, Qld, 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16548.pdf.

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4

Nita, Mizuho. "Epidemiology and management of Phomopsis cane and leaf spot of grape." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1125542230.

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Thesis (Ph. D.)--Ohio State University, 2005.
Title from first page of PDF file. Document formatted into pages; contains xviii, 163 p.; also includes graphics (some col.). Includes bibliographical references (p. 156-163). Available online via OhioLINK's ETD Center
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5

Mostert, Lizel. "The characterization and control of Phomopsis cane and leaf spot on vine." Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51945.

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Thesis (MScAgric.)--Stellenbosch University, 2000.
ENGLISH ABSTRACT: Phomopsis cane and leaf spot disease of grapevine is an economically important disease in many of the vine-growing areas of the world. Four different Phomopsis spp. have previously been associated with this disease. The present study investigates the taxonomic significance of the different taxa found on grapevines in South Africa, as well as the endophytic growth and fungicide sensitivity of Phomopsis viticola isolates. The thesis is compiled of several different parts, which deal with specific, but related topics, and hence some duplication has been unavoidable. Understanding the epidemiology of a disease is important for the correct timing of disease control. To investigate the endophytic growth of P. viticola, asymptomatic shoots were collected at eight different growth stages. Nodes, internodes, leaf petioles, leaves, tendrils and bunch peduncles were investigated. Two Phomopsis spp., taxon 1 and 2 were identified in this study. The Phomopsis viticola-complex had a relative importance of 9% and accounted for 3% of the isolations. P. viticola (taxon 2) is mainly isolated from the nodes and internodes. Inoculations of healthy, young vine tissue confirmed taxon 2 to be a virulent pathogen, suggesting that it is a latent pathogen rather than an endophyte. In contrast, taxon 1 appeared to be a true endophyte, and did not seem to be an important pathogen on vines. The true identity of the causal organism of Phomopsis cane and leaf spot disease was investigated by collecting samples from 58 different vineyards in the grapevine growing areas of the Western Cape. P. viiicola occurred in grapevine material collected from Lutzville to Swellendam, but was not found in the Oudtshoorn and Orange River grapevine areas. Diaporthe perjuncta (taxon 1), P. vutcola (taxon 2), taxon 3 and a Phomopsis species commonly associated with shoot blight of peaches in the U.S.A., P. amygdali, were identified among the South African grapevine isolates. Examination of the Australian culture designated as taxon 4 found it to be a species of Libertella, thus excluding it from the P. viticola-complex. An Italian isolate was found to represent a species of Phomopsis not previously known from grapevines, and this was subsequently described as taxon 5. Species delimitation was based on morphological and cultural characteristics, stem inoculations and the formation of the teleomorph in vitro. The identity of each morphological taxon was confirmed by means of phylogenetic analyses of the nuclear ribosomal DNA internal transcribed spacers (ITS 1 and ITS2) and the 5' end partial sequence of the mitochondrial small subunit (mtSSU). P. amygdali, associated with peach shoot blight in the U.S.A., was isolated once only and appeared to be of lesser importance in this disease complex. Furthermore, taxa 1 (Diaporthe perjuncta) and 3 were also rarely encountered and proved to be non-pathogenic, indicating their non-functional role in Phomopsis cane and leaf spot disease. Taxon 2 (Phomopsis viticolas was common and widely distributed in diseased vineyards. This taxon was associated with the typical disease symptoms and proved to be pathogenic. Morphologically taxon 2 corresponded best with P. viticola, which was also neotypified in this study. Taxon 2 was mostly isolated from buds and nodes, indicating that these are important sites in which the fungus survives during winter. Molecular data indicated that taxon 3 and P. amygdali were not host specific to grapevine. The currently used foliar fungicides were compared to the new strobilurin fungicides. The effects of nine fungicides (azoxystrobin, flusilazole, folpet, fosetyl- Al+mancozeb, kresoxim-methyl, mancozeb, penconazole, spiroxamine and trifloxystrobin) were tested in vitro on inhibition of mycelial growth. The following EC50 (ug/ml) values were obtained: azoxystrobin (0.350), flusilazole (0.007), folpet (4.489), fosetyl-Al+mancozeb (3.925), kresoxim-methyl (1.665), mancozeb (2.891), penconazole (0.023), spiroxamine (0.321) and trifloxystrobin (0.051). Additionally, azoxystrobin, folpet, kresoxim-methyl, mancozeb, propineb and trifloxystrobin were tested for their ability to inhibit spore germination in vitro. The subsequent EC50 (ug/ml) values were obtained: azoxystrobin 0.123), folpet (0.510), kresoxim-methyl (0.0037), mancozeb (0.250), propineb (0.156) and trifloxystrobin (0.003). The results reported in part 4 showed that the strobilurin fungicides inhibited the mycelial growth and spore germination of P. viticola. However, further trials need to be conducted to verify these findings under field conditions. In the present study taxa 1, 3 and P. amygdali were infrequently isolated, suggesting that they played a less prominent role in the P. viticolacomplex.
AFRIKAANSE OPSOMMING: Streepvleksiekte van wingerd is 'n ekonomies belangrike siekte wat in die meeste wingerdproduserende gebiede van die wêreld voorkom. Vier Phomopsis spesies is in die verlede met dié siekte geassosieer. Hierdie studie ondersoek die taksonomiese belangrikheid van die verskillende taksa wat op wingerd in Suid Afrika gevind word, asook die endofietiese groei en fungisiedsensitiwiteit van die Phomopsis vitico/a isolate. Hierdie tesis bestaan uit verskeie dele met spesifieke, maar verwante onderwerpe wat tot onafwendbare duplisering lei. Dit is belangrik om die epidemiologie van 'n siekte te verstaan sodat korrekte en tydsberekende siektebeheer toegepas kan word. Die endofietiese groei van P. vitico/a is ondersoek deur simptoomlose lote by agt verskillende groei stadiums te versamel. Nodusse, internodusse, blaarstele, blare, rankies en trosstele is ondersoek. Twee Phomopsis spp., takson 1 en 2 is geïdentifiseer. Die Phomopsis vitico/a-kompleks het 3% van die isolasies uitgemaak en 'n relatiewe belangrikheid van 9% getoon. P. vitico/a (takson 2) is meestal uit die nodus en internodus geïsoleer. lnokulasies van gesonde, jong wingerdweefsel het bevestig dat takson 2 'n virulente patogeen is en dat die takson eerder 'n latente patogeen as 'n endofiet is. In teenstelling hiermee is takson 1 'n ware endofiet en 'n onbelangrike patogeen op wingerd. Die ware identiteit van die veroorsakende organisme van streepvlek is ondersoek deur plantmateriaal vanaf 58 verskillende wingerde in die wingerproduserende gebiede van die Wes-Kaap te versamel. P. vitico/a is in wingerdmateriaal vanaf Lutzville tot Swellendam aangetref, maar nie in die Oudtshoorn en Oranjerivier wingerd produserende gebiede nie. Diaporthe perjuncta (takson 1), P. vitico/a (takson 2), takson 3 en P. amygdali is in die Suid Afrikaanse wingerdisolate geïdentifiseer. P. amygdali word met lootverskroeiing van perske bome in die V.S.A. geassosieer. Die Australiese isolaat wat benoem is as takson 4, is met die huidige ondersoek gevind om 'n spesie van Libertella te wees. Takson 4 is daarvolgens uit die P. vitico/a-kompleks gelaat. 'n Italiaanse isolaat het 'n nuwe spesie van Phomopsis op wingerd verteenwoordig en is vervolgens as takson 5 beskryf. Spesie-onderskeiding is op morfologiese en kulturele eienskappe, staminokulasies en die vorming van die teleomorf in vitro gebaseer. Die identiteit vanelke morfologiese takson is met behulp van filogenetiese analises van die nukleêre ribosomale DNS intern transkriberende spasieerders (ITS 1 en ITS2) en die 5' punt gedeeltelike nukleotied volgorde van die mitochondriale klein subeenheid (mtSSU) bevestig. P. amygdali is slegs een keer geïsoleer en blyk van minder belang in die siektekompleks te wees. Takson 1 (Diaporthe perjuneta) en takson 3 het ook min voorgekom en is nie-patogenies, wat hul nie-funksionele rol in streepvleksiekte aandui. Takson 2 (P. viticola) is algemeen geïsoleer en kom wyd verspreid voor. Hierdie takson is geassosieer met die tipiese siektesimptome en is ook patogenies. Morfologies stem takson 2 met P. viiicola ooreen en is ook geneotipifiseer in hierdie studie. Takson 2 is meestal vanaf die ogies en nodusse geïsoleer, wat daarop dui dat hierdie belangrike setels is waar die swam tydens die winter oorleef. Die molekulêre data toon aan dat takson 3 en P. amygdali nie gasheerspesifiek tot wingerd is nie. Die swamdoders wat tans teen streepvlek gebruik word, is met die nuwe strobilurin swamdoders vergelyk. Die effek van nege swamdoders (azoksistrobin, flusilasool, folpet, fosetyl-Al + mancozeb, kresoxirn-metiel, mankozeb, penconasool, spiroksamien en trifloksistrobin) is in vitro op die inhibisie van miseliumgroei getoets. Die volgende EKso-waardes (g/ml) is verkry: azoxystrobin (0.350), flusilasool (0.007), folpet (4.489), fosetiel-Al + mankozeb (3.925), kresoxirn-metiel (l.665), mankozeb (2.891), penkonasool (0.023), spiroksamien (0.321) en trifloxystrobin (0.051). Azoxystrobin, folpet, kresoxim-rnetiel, mankozeb, propineb en trifloksistrobin is ook in vitro getoets vir hul inhibisie op spoorontkieming. Die volgende EKso-waardes is verkry: azoxystrobin (0.123), folpet (0.510), kresoxim-metiel (0.0037), mankozeb (0.250), propineb (0.156) en trifloxystrobin (0.003). Die resultate vervat in deel 4 toon dat die strobilurin swamdoders die miseliumgroei en spoorontkieming van P. viticola inhibeer. Toetsing in die veld word egter benodig om die effektiwiteit van die middels te bevestig. In hierdie studie is taksa I, 3 en P. amygdali selde geïsoleer, wat aangedui het dat hierdie taksa 'n minder belangrike rol in die P. viticola-kompleks speel.
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Garcia, Armando da Costa, and 92-99157-4536. "Avaliação química e biológica do extrato bruto do fungo endofítico Phomopsis sp." Universidade Federal do Amazonas, 2017. http://tede.ufam.edu.br/handle/tede/6219.

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FAPEAM - Fundação de Amparo à Pesquisa do Estado do Amazonas
Endophytic fungi are microorganisms that live in interne tissues of plants, without causing them immediate damage. They are considered an important source to the production of secondary metabolites with antimicrobial, antitumor activities, among others. The present work describes the chemical and biological study of the secondary metabolites produced by the endophytic fungus Phomopsis sp. isolated from the plant Passovia stelis. For that, the fungus at hand was cultivated in large scale using the liquid medium of dextrose potato. The ethylacetat crude extract was obtained through extraction from the broth and then submitted to larvicide assay against larvae of the mosquito Aedes aegypti and antimicrobial against the pathogenic bacteria Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. It was also performed assays against the enzyme anticholinesterase, antioxidants and antifungal against Cladosporium cladosporioides e Cladosporium sphaerospermum. In the selective larvicide, 3 fractions showed bioactivity (100 ppm) in 24h. In the dosing assay larvicide, the extract of the fungus Phomopsis sp. showed bioactivity with LD50 of 84.7, 81.6 and 79.5 ppm and LD90 de 147.8, 140.2 and 137.7 ppm after 24, 48 e 72 h, respectively. The crude extract was analyzed using TLC, HPLC and NMR and then purified through column chromatography and HLPC-DAD. From F4, the 3-nitropropionic acid was isolated, which is used in neurological research. Succinic acid, which is considered one of the construction blocks in chemical synthesis industry, was isolated from the fraction F7. Other 3 esterified substances, derivatives of tyrosol, were isolated of the fraction F1, and one of them unpublished. In the antioxidant assay, two fractions (F5, F6) showed positive results and in the assay against the enzyme anticholinesterase four fraction (F2, F5, F6, F7) presented bioactivity. These results demonstrate the expressive biopotency of the fungus Phomopsis sp. and open a new perspective for further studies.
Fungos endofíticos são fungos que vivem no tecido interno das plantas sem que lhes causem prejuízo imediato. Esses microrganismos são considerados uma fonte importante para a produção de metabólitos secundários com ação antimicrobiana, antitumoral, entre outras. Este trabalho descreve o estudo químico e biológico dos metabólitos secundários produzidos pelo fungo endofítico Phomopsis sp. isolado da planta Passovia stelis. Para tanto, o fungo em questão foi cultivado em escala ampliada, utilizando meio líquido de batata dextrose. O caldo obtido foi extraído com acetato de etila fornecendo o extrato bruto. Este foi submetido à ensaio larvicida, frente as larvas do mosquito Aedes aegypti, antimicrobiano frente às bactérias patogênicas Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli e Pseudomonas aeruginosa. Também foram realizados ensaios anticolinesterase, antioxidante e antifúngico frente aos fungos Cladosporium cladosporioides e Cladosporium sphaerospermum. No ensaio larvicida de dose, o extrato do fungo Phomopsis sp. exibiu bioatividade com valores de DL50 de 84,7; 81,6 e 79,5 ppm e DL90 de 147,8; 140,2 e 137,7 ppm respectivamente, nas leituras realizadas após 24, 48 e 72 h. No ensaio larvicida seletivo, três frações obtiveram bioatividade em 100 ppm nas primeiras 24 horas. Após análise por CCD, CLAE e RMN, o extrato bruto foi fracionado por cromatografia em coluna e por CLAE-DAD. Da fração F4 foi isolado o ácido 3-nitropropiônico que é muito utilizado em pesquisas neurológicas. Da fração F7 foi isolado o ácido succínico tido como um dos principais blocos de construção na indústria da síntese química. Outras três substâncias da classe dos ésteres, derivados do tirosol foram isolados da fração F1 sendo uma delas inédita. No ensaio antioxidante duas frações (F5, F6) deram resultado positivo e no ensaio anticolinesterásico quatro frações (F2, F5, F6, F7) mostraram atividade. Estes resultados mostraram a expressiva biopotência do fungo Phomopsis sp, abrindo novas perspectivas atrativas para estudos futuros.
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Yu, Haiqian [Verfasser]. "Secondary Metabolites from Fungi Coniella fragariae and Phomopsis sp. D15a2a / Haiqian Yu." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2020. http://d-nb.info/120891507X/34.

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8

Chapla, Vanessa Mara [UNESP]. "Estudo químico e biológico do fungo endofítico Phomopsis sp. isolado da Senna spectabilis." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/97895.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Micro-organismos endofíticos habitam os espaços intra e intercelular de um hospedeiro, durante todo ou parte de seu ciclo de vida, sem causar danos aparentes. Esses micro- organismos são considerados uma fonte importante para a produção de metabólitos secundários com ação antimicrobiana, antitumoral, entre outras. Este trabalho descreve o estudo químico e biológico do fungo endofítico Phomopsis sp. associado à espécie vegetal do cerrado Senna spectabilis. Phomopsis sp. foi cultivado em diferentes meios de cultura para avaliar a variação metabólica em relação a composição dos nutrientes. Foram usados 5 meios líquidos comerciais (MBD, YM, Extrato de Malte, Nutrient e Czapek) e 2 meios sólidos (Milho e Arroz). Os extratos brutos Acetato e Acetonitrila obtidos foram avaliados por Cromatografia Líquida de Alta Eficiência com detector de arranjo de diodos e por Ressonância Magnética Nuclear de 1 H, e submetidos aos ensaios para a avaliação da potencialidade antifúngica, antioxidante e anticolinesterásica. Os resultados evidenciaram extratos com uma variação metabólica intensa e potencialmente bioativos. Destes, os meios MDB e Milho foram selecionados para obtenção dos extratos brutos em escala ampliada. O extrato bruto acetato obtido em MDB foi submetido a fracionamento cromatográfico e levou à identificação da uracila (1), ácido nitropropanóico (2), citocalasina H (3), tirosol (4), ciclo(L- Pro-L-Leu), (5), ciclo(L-Pro-L-Ile) (6), ciclo(L-Pro-L-Val) (7), ciclo(L-Pro-L-Tyr) (8) e ciclo(L-Pro-L-Phe) (9). Este é o primeiro relato de dicetopiperazinas obtidos de fungos do gênero Phomopsis. Esta classe de substâncias apresenta inúmeras bioatividades, principalmente antimicrobiana. O extrato bruto acetonitrila obtido de milho conduziu ao isolamento da citocalasina H (3), citocalasina J (10), alternariol monometil éter (11).... não descritos
Endophytic microorganisms inhabit the intra and intercellular spaces of a host, during all or part of their life cycle, without causing any apparent damage. Microorganisms are regarded as an important source for the production of secondary metabolites with antimicrobial, antitumoral actions, among others. This work describes the chemical and biological study of endophytic fungus Phomopsis sp. associated with plant species of Cerrado Senna spectabilis. Phomopsis sp. was cultived in different culture media to assess the metabolic variation in relation to nutrient composition. Were used 5 commercial liquid media (PDB, YM, Malt extract, nutrient and Czapek) and 2 solid mediums (Corn and Rice). The acetate and acetonitrile crude extracts were evaluated by High Performance Liquid Chromatography with Diode Array Detector and 1 H Nuclear Magnetic Resonance and subjected to assays for the evaluation of potential antifungal, antioxidant and anticholinesterase. The results showed extracts with an intense metabolic variation and potentially bioactive. From these, the mediums PDB and Corn were selected to obtain the crude extracts on a large scale. The crude acetate extract obtained in PDB was submitted to chromatographic separation and led to the identification of uracil (1), 3-nitropropionic acid (2), cytochalasin H (3), tyrosol (4), cyclo (L- Pro-L-Leu) (5), cyclo (L-Pro-L-Ile) (6), cyclo (L-Pro-L-Val) (7), cyclo (L-Pro-L-Tyr) (8) and cyclo (L-Pro-L-Phe) (9). This is the first report of diketopiperazines obtained from fungi of the Phomopsis genus. This class of substances shows many bioactivities, especially antimicrobial. The crude acetonitrile extract obtained from Corn led to the isolation of cytochalasin H (3), cytochalasin J (10), alternariol monomethyl ether (11), alternariol (12) and 2-hydroxy-alternariol (13), being the last one reported for the first time as a natural product and with NMR data not reported
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Morin, Louise. "Development of the field bindweed bioherbicide, Phomopsis convolvulus : spore production and disease development." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59614.

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Phomopsis convolvulus Ormeno, a foliar pathogen of field bindweed, is a good candidate to be developed as a bioherbicide. Large numbers of infective propagules were produced in shake-flask liquid fermentation with modified Richard's (V-8) medium and in solid-substrate fermentation with pearl barley grains. In complex liquid media, pycnidium-like structures were observed. Most conidia stored at $-$70$ sp circ$C remained viable and virulent for at least six months.
In controlled environment studies, a minimum of 18 hr of dew was required for severe disease development on inoculated plants. The addition of gelatin, Sorbo $ sp{ rm TM}$, or BOND$ sp{ rm TM}$ to the inoculum did not enhance the disease under various leaf wetness periods. A continuous dew period of 18 hr was superior to the cumulative effect of three interrupted 6 hr dew periods. Secondary inoculum was produced on diseased plants placed under moist conditions for 48 hr or more.
In greenhouse experiments, seedlings at the cotyledon and 3- to 5- leaf stage were severely diseased and killed when inoculated with 10$ sp9$ conidia/m$ sp2$. This inoculum density adversely affected the regenerative ability of 4 wk old seedlings and established plants, but few plants were killed. Inoculation of the healthy regrowth from plants previously inoculated with the fungus resulted in much less disease symptoms than expected.
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Chapla, Vanessa Mara. "Estudo químico e biológico do fungo endofítico Phomopsis sp. isolado da Senna spectabilis /." Araraquara : [s.n.], 2010. http://hdl.handle.net/11449/97895.

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Orientador: Ângela Regina Araújo
Banca: Dulce Helena Siqueira Silva
Banca: Jacqueline Aparecida Takahashi
Resumo: Micro-organismos endofíticos habitam os espaços intra e intercelular de um hospedeiro, durante todo ou parte de seu ciclo de vida, sem causar danos aparentes. Esses micro- organismos são considerados uma fonte importante para a produção de metabólitos secundários com ação antimicrobiana, antitumoral, entre outras. Este trabalho descreve o estudo químico e biológico do fungo endofítico Phomopsis sp. associado à espécie vegetal do cerrado Senna spectabilis. Phomopsis sp. foi cultivado em diferentes meios de cultura para avaliar a variação metabólica em relação a composição dos nutrientes. Foram usados 5 meios líquidos comerciais (MBD, YM, Extrato de Malte, Nutrient e Czapek) e 2 meios sólidos (Milho e Arroz). Os extratos brutos Acetato e Acetonitrila obtidos foram avaliados por Cromatografia Líquida de Alta Eficiência com detector de arranjo de diodos e por Ressonância Magnética Nuclear de 1 H, e submetidos aos ensaios para a avaliação da potencialidade antifúngica, antioxidante e anticolinesterásica. Os resultados evidenciaram extratos com uma variação metabólica intensa e potencialmente bioativos. Destes, os meios MDB e Milho foram selecionados para obtenção dos extratos brutos em escala ampliada. O extrato bruto acetato obtido em MDB foi submetido a fracionamento cromatográfico e levou à identificação da uracila (1), ácido nitropropanóico (2), citocalasina H (3), tirosol (4), ciclo(L- Pro-L-Leu), (5), ciclo(L-Pro-L-Ile) (6), ciclo(L-Pro-L-Val) (7), ciclo(L-Pro-L-Tyr) (8) e ciclo(L-Pro-L-Phe) (9). Este é o primeiro relato de dicetopiperazinas obtidos de fungos do gênero Phomopsis. Esta classe de substâncias apresenta inúmeras bioatividades, principalmente antimicrobiana. O extrato bruto acetonitrila obtido de milho conduziu ao isolamento da citocalasina H (3), citocalasina J (10), alternariol monometil éter (11).... não descritos
Abstract: Endophytic microorganisms inhabit the intra and intercellular spaces of a host, during all or part of their life cycle, without causing any apparent damage. Microorganisms are regarded as an important source for the production of secondary metabolites with antimicrobial, antitumoral actions, among others. This work describes the chemical and biological study of endophytic fungus Phomopsis sp. associated with plant species of Cerrado Senna spectabilis. Phomopsis sp. was cultived in different culture media to assess the metabolic variation in relation to nutrient composition. Were used 5 commercial liquid media (PDB, YM, Malt extract, nutrient and Czapek) and 2 solid mediums (Corn and Rice). The acetate and acetonitrile crude extracts were evaluated by High Performance Liquid Chromatography with Diode Array Detector and 1 H Nuclear Magnetic Resonance and subjected to assays for the evaluation of potential antifungal, antioxidant and anticholinesterase. The results showed extracts with an intense metabolic variation and potentially bioactive. From these, the mediums PDB and Corn were selected to obtain the crude extracts on a large scale. The crude acetate extract obtained in PDB was submitted to chromatographic separation and led to the identification of uracil (1), 3-nitropropionic acid (2), cytochalasin H (3), tyrosol (4), cyclo (L- Pro-L-Leu) (5), cyclo (L-Pro-L-Ile) (6), cyclo (L-Pro-L-Val) (7), cyclo (L-Pro-L-Tyr) (8) and cyclo (L-Pro-L-Phe) (9). This is the first report of diketopiperazines obtained from fungi of the Phomopsis genus. This class of substances shows many bioactivities, especially antimicrobial. The crude acetonitrile extract obtained from Corn led to the isolation of cytochalasin H (3), cytochalasin J (10), alternariol monomethyl ether (11), alternariol (12) and 2-hydroxy-alternariol (13), being the last one reported for the first time as a natural product and with NMR data not reported
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Книги з теми "Phomopsis"

1

Harris, Jeri Lyn. Phomopsis blight at Bessey Nursery. Golden, CO: USDA Forest Service, Rocky Mountain Region, Renewable Resources, 1999.

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2

A world list of Phomopsis names with notes on nomenclature, morphology, and biology. Berlin: J. Cramer, 1988.

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3

Food, Ontario Ministry of Agriculture and. Phomopsis cane and leaf spot of grapes. S.l: s.n, 1990.

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4

Funk, A. Phomopsis (Diaporthe) canker of Douglas-fir in British Columbia. Victoria, B.C: Government of Canada, Canadian Forestry Service, Pacific Forestry Centre, 1986.

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Fla.) Conference on the Diaporthe/Phomopsis Disease Complex of Soybean (1984 Fort Walton Beach. Proceedings of the Conference on the Diaporthe/Phomopsis Disease Complex of Soybean: March 26-27, 1984, Fort Walton Beach, Florida. Washington, D.C.?]: U.S. Dept. of Agriclture, Agricultural Research Service, 1985.

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Частини книг з теми "Phomopsis"

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Thomison, P. R. "Factors Affecting the Severity of Phomopsis Seed Decay in Soybeans." In World Soybean Research Conference III: Proceedings, 495–502. New York: CRC Press, 2022. http://dx.doi.org/10.1201/9780429267932-84.

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Ponmurugan, P., and D. Saravanan. "Evaluation of Streptomyces spp. for the Biological Control of Phomopsis Canker Disease of Tea Plants." In Microbiological Research In Agroecosystem Management, 197–206. India: Springer India, 2013. http://dx.doi.org/10.1007/978-81-322-1087-0_14.

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Moricca, Salvatore, Gloria Innocenti, and Alessandro Ragazzi. "Epidemiological Investigations Shed Light on the Ecological Role of the Endophyte Phomopsis quercina in Mediterranean Oak Forests." In Endophytes of Forest Trees, 207–27. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-89833-9_10.

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"Phoma and Phomopsis." In Molecular Detection of Human Fungal Pathogens. CRC Press, 2011. http://dx.doi.org/10.1201/b11375-18.

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Li, Shuxian. "Phomopsis Seed Decay of Soybean." In Soybean - Molecular Aspects of Breeding. InTech, 2011. http://dx.doi.org/10.5772/15688.

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Tsantrizos, Youla S. "Bioactive metabolites of the genus Phomopsis." In Structure and chemistry (Part C), 341–59. Elsevier, 1995. http://dx.doi.org/10.1016/s1572-5995(06)80136-5.

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"Diaporthe/Phomopsis helianthi, the sunflower pathogen: twenty years of research—Jelena Vukojevic,." In Environment and Crop Production, 119–30. CRC Press, 2002. http://dx.doi.org/10.1201/9781482280012-20.

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Nand Tripathi, Atma, Shailesh Kumar Tiwari, and Tushar Kanti Behera. "Postharvest Diseases of Vegetable Crops and Their Management." In Postharvest Technology - Recent Advances, New Perspectives and Applications [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.101852.

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Анотація:
Vegetable crops have an important role in food and nutrition and maintain the health of soil. India is the second-largest producer of vegetables in the world with a 16% (191.77 MT) share of global vegetable production. Every year, diseases cause postharvest losses (40–60%) in vegetable crops due to their perishable nature under field (15–20%), packaging and storage (15–20%), and transport (30–40%). Profiling, detection, and diagnosis of postharvest vegetable pathogens (diseases) are essential for better understanding of pathogen and formulation of safe management of postharvest spoilage of vegetables. The vegetable produce is spoiled by postharvest pathogens and makes them unfit for human consumption and market due to the production of mycotoxins and other potential human health risks. Genera of fungal pathogens viz. Alternaria, Aschochyta, Colletotrichum, Didymella, Phoma, Phytophthora, Pythium, Rhizoctonia, Sclerotinia, Sclerotium, and bacterial pathogens viz. Erwinia spp., Pseudomonas spp., Ralstonia solanacearum, Xanthomonas euvesictoria were recorded as postharvest pathogens on vegetable crops. Fruit rot incidence of several post-harvest pathogens viz. Alternaria solani (30%), Phytophthora infestans (15%), Rhophitulus solani (30%), Sclerotium rolfsii (30%) fruit rot and X. euvesictoria (5%) canker on tomato; Colletotrichum dematium fruit rot (20%) on chili; Phomopsis vexans (60%) fruit rot on brinjal was recorded. Didymella black rot and Colletotrichum anthracnose were recorded on fruits of bottle gourd, pumpkin, ash gourd, and watermelon. Important leguminous vegetable crops are infected by postharvest pathogens viz. Ascochyta pisi, Colletotrichum lindemuthianum (Anthracnose), Sclerotinia sclerotiorum (white rot) and Pseudomonas syringae pv. phaseolicola (blight), Sclerotinia white rot, Alternaria blight. However, Xanthomonas black rot (10%) on cabbage and Pectinovora (Erwinia) soft rot (19%) were recorded as emerging post-harvest pathogens on cauliflower.
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Тези доповідей конференцій з теми "Phomopsis"

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Zibrov, A. V., and N. A. Bushneva. "THE EFFECT OF THE SOWING DATES ON PLANT AFFECTION WITH SUNFLOWER PHOMOPSIS BLIGHT." In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-174-177.

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We studied the effect of the sowing dates on the prevalence and development of sunflower Phomopsis blight. We preliminary established that when sunflower is sown in the first ten days of April, the Phomopsis blight affection degree was the highest (P – 4.1 %, R – 2 points), and in case of sowing in the first ten days of May the affection degree was minimal (P – 0.7 %, R – 1 point).
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McGee, Denis C. "Pod Test for Phomopsis Seed Decay of Soybean." In Proceedings of the First Annual Crop Production and Protection Conference. Iowa State University, Digital Press, 1989. http://dx.doi.org/10.31274/icm-180809-301.

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Mathew, Febina M., and Samuel G. Markell. "Insights into the Diaporthe/Phomopsis complex infecting soybeans in the United States." In Proceedings of the 24th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2014. http://dx.doi.org/10.31274/icm-180809-153.

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Soto-Arias, J. P., Alison Robertson, Matthew E. O'Neal, and Gary Munkvold. "Virus-Phomopsis interactions on soybean and the effects of insect and disease management practices." In Proceedings of the 28th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2010. http://dx.doi.org/10.31274/icm-180809-263.

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Yang, X. B. "Biology and Management of Premature Yellowing and Death of Soybean Caused by Phomopsis Idiaporthe Fungi." In Proceedings of the 13th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2001. http://dx.doi.org/10.31274/icm-180809-719.

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Звіти організацій з теми "Phomopsis"

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Mueller, Daren, Carl Bradley, Martin Chilvers, Loren Giesler, Febina Mathew, Damon Smith, Kiersten Wise, et al. Pod and Stem Blight and Phomopsis Seed Decay. United States: Crop Protection Netework, June 2015. http://dx.doi.org/10.31274/cpn-20190620-016.

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Soto-arias, Jose Pablo, and Gary P. Munkvold. Bean Leaf Beetle-Phomopsis Management Trials on Soybean. Ames: Iowa State University, Digital Repository, 2010. http://dx.doi.org/10.31274/farmprogressreports-180814-420.

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Petrovic, Kristina, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory, et al. New Fungi Discovered Causing Phomopsis Seed Decay of Soybean. Ames (Iowa): Iowa State University. Library, August 2021. http://dx.doi.org/10.31274/cpn-20210820-0.

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Petrovic, Kristina, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory, et al. New Fungi Discovered Causing Phomopsis Seed Decay of Soybean. United States of America: Crop Protection Netework, August 2021. http://dx.doi.org/10.31274/cpn-20210825-0.

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Petrovic, Kristina, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory, et al. New Fungi Discovered Causing Phomopsis Seed Decay of Soybean. United States of America: Crop Protection Netework, August 2021. http://dx.doi.org/10.31274/cpn-20210826-000.

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van den Top, H. J., and J. G. J. Mol. Determination of phomopsin-A in lupin seeds and lupin-derived products : results of an interlaboratory validation study. Wageningen: RIKILT Wageningen University & Research, 2017. http://dx.doi.org/10.18174/410802.

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