Добірка наукової літератури з теми "Phage displayed scFv"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Phage displayed scFv".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Статті в журналах з теми "Phage displayed scFv"
Zhao, Peng, Guijie Zhu, Lihua Zhang, Zhen Liang, Zonghai Li, and Yukui Zhang. "New method for determination of average scFv fragment number displayed on the M13 phage surface." Pure and Applied Chemistry 82, no. 1 (January 3, 2010): 205–11. http://dx.doi.org/10.1351/pac-con-09-02-03.
Повний текст джерелаBrettschneider, Kerstin, Anja Naumann, Sonja Neimanis, Joerg Kahle, Christine Heller, Thomas Klingebiel, Dirk Schwabe, and Christoph Koenigs. "Functional Analysis of Phage Display Selected Single-Chain Variable Antibody Fragments (scFvs) Specific for Anti-FVIII Antibodies." Blood 124, no. 21 (December 6, 2014): 1499. http://dx.doi.org/10.1182/blood.v124.21.1499.1499.
Повний текст джерелаGoswami, Pooja, Deepti Saini, and Subrata Sinha. "Phage Displayed scFv: pIII Scaffold May Fine Tune Binding Specificity." Hybridoma 28, no. 5 (October 2009): 327–31. http://dx.doi.org/10.1089/hyb.2009.0008.
Повний текст джерелаMuller, Bruno H., Florence Lafay, Caroline Demangel, Pierre Perrin, Noël Tordo, Anne Flamand, Pierre Lafaye, and Jean-Luc Guesdon. "Phage-displayed and soluble mouse scFv fragments neutralize rabies virus." Journal of Virological Methods 67, no. 2 (September 1997): 221–33. http://dx.doi.org/10.1016/s0166-0934(97)00099-2.
Повний текст джерелаWind, Troels, Brian Stausbøl-Grøn, Svend Kjær, Liselotte Kahns, Kristian H. Jensen, and Brian F. C. Clark. "Retrieval of phage displayed scFv fragments using direct bacterial elution." Journal of Immunological Methods 209, no. 1 (November 1997): 75–83. http://dx.doi.org/10.1016/s0022-1759(97)00151-8.
Повний текст джерелаvan den Brink, Edward N., Ellen A. M. Turenhout, Julian Davies, Niels Bovenschen, Karin Fijnvandraat, Willem H. Ouwehand, Marjolein Peters, and Jan Voorberg. "Human antibodies with specificity for the C2 domain of factor VIII are derived from VH1 germline genes." Blood 95, no. 2 (January 15, 2000): 558–63. http://dx.doi.org/10.1182/blood.v95.2.558.
Повний текст джерелаNaumann, Anja, Jörg Kahle, Nadia Reiss, Dirk Schwabe, Christine Heller, Thomas Klingebiel, and Christoph Königs. "Development of a Factor VIII-Specific Immunotherapy for Hemophilia A." Blood 120, no. 21 (November 16, 2012): 3361. http://dx.doi.org/10.1182/blood.v120.21.3361.3361.
Повний текст джерелаStuknytė, Milda, Eeva-Christine Brockmann, Tuomas Huovinen, Simone Guglielmetti, Diego Mora, Valentina Taverniti, Stefania Arioli, Ivano De Noni, and Urpo Lamminmäki. "Lactobacillus helveticus MIMLh5-Specific Antibodies for Detection of S-Layer Protein in Grana Padano Protected-Designation-of-Origin Cheese." Applied and Environmental Microbiology 80, no. 2 (November 15, 2013): 694–703. http://dx.doi.org/10.1128/aem.03057-13.
Повний текст джерелаSchmidt, Anja, Kerstin Brettschneider, Jörg Kahle, Aleksander Orlowski, Karin Becker-Peters, Diana Stichel, Jörg Schulze, et al. "Neutralisation of factor VIII inhibitors by anti-idiotypes isolated from phage-displayed libraries." Thrombosis and Haemostasis 116, no. 07 (January 2016): 32–41. http://dx.doi.org/10.1160/th15-12-0925.
Повний текст джерелаMassion, P. P., T. V. Pedchenko, D. V. Parekh, and R. Mernaugh. "Selection of lung cancer-associated scFv antibodies from cultured cells." Journal of Clinical Oncology 27, no. 15_suppl (May 20, 2009): e22137-e22137. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.e22137.
Повний текст джерелаДисертації з теми "Phage displayed scFv"
Gomes, Carlos Henrique Rodrigues. "Construção de uma bibilioteca de anticorpos ScFv dirigidos contra o fator de crescimento vascular (VEGF)." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-09082013-093807/.
Повний текст джерелаAngiogenesis is the formation of new blood vessels from pre-existing ones and is an important physiological process, which in adults is mostly restricted to wound healing or the female reproductive cycle. However, different illnesses, such as cancer or retinopathies, induce the formation of pathological angiogenesis, necessary for disease progression. Monoclonal antibodies are one of the fastest growing class of biopharmaceuticals with important implications in angiogenesis dependent diseases. Among various methods for the identification of monoclonal antibodies against therapeutic targets is phage display technology. Because the vascular endothelial growth factor (VEGF) is the main molecular factor responsible for the formation of new blood vessels, the major anti-angiogenic drug available in the clinic today is a monoclonal antibody (bevacizumab) directed against VEGF. However, although anti-VEGF therapies are effective, they are not yet ideal due to undesirable side effects and drug resistance. Novel alternatives are necessary to improve on angiogenic therapies. The aim of our study is to identify novel molecular targets and to develop new therapeutic agents for angiogenic dependent diseases. To achieve our goal we have chosen the phage display system in order to select for antibodies with angiogenic properties. An antibody phage library has been developed in our laboratory, directed against VEGF molecule, particularly one of it isoforms. The animals were immunized and developed specific antibodies, detected by ELISA and Western-blot. Amplification of the pool of light and heavy chain Ig genes was performed to produce the single chain (ScFv) fragments for library construction. The ScFv antibody display libraries will be then screened in angiogenic settings to isolate antibodies against specific VEGF isoforms and novel cell surface molecular markers expressed by activated human endothelial cells
Moutel, Sandrine. "Sélection et amélioration d'anticorps recombinants, applications à la recherche fondamentale et à l'immunothérapie." Paris 6, 2009. http://www.theses.fr/2009PA066520.
Повний текст джерелаMuller, Benjamin. "Création d'une banque de scFv-phages ciblant des protéines hydrophiles ou membranaires." Thesis, Montpellier 1, 2014. http://www.theses.fr/2014MON13511.
Повний текст джерелаNowadays, more than 60% of marketed drugs target membrane proteins. However, their study still represents a challenge, essentially due to their particular 3D-structure (hydrophobic transmembrane domains and hydrophilic extra- and intra-cellular domains), but also to their low expression level in cells.Ciloa, the start-up company in which I realized my PhD, has developed a patented technology that enables to express native membrane proteins on exosomes, membrane vesicles of 30 to 100nm, using a pilot peptide called DCTM (for Cytosolic Domain of TransMembrane). This technology displays a lot of different applications, in different domains such as drug screening, vaccines development or monoclonal antibodies (mAbs) development.The purpose of my PhD research was, first, to set up the recombinant exosomal tool using Ciloa's innovative technology, and then to use this tool to develop monoclonal antibodies.Thus, at the beginning of my PhD, I set up exosomal characterization technics, such as ELISA, and I also took part in the setup of several production and purification protocols, depending of the use of exosomes. Once these tools had been optimized, I was able to use them to develop mAbs. I tested two methods, one classical, the generation of hybridoma after Balb/c mice immunizations, and a more recent technology, the screening of scFvs library by phage display.Therefore, I obtained hybridoma and was able to screen the derived antibodies by ELISA on exosomes. Concerning the phage display technology, I took part in the development of a new scFvs library, based on the 13R4 scaffold, of which we changed the CDRs lengths, mostly the CDRH3, in order to target epitopes with low accessibility, such as the one of membrane proteins. The library screening was realized on recombinant exosomes
Japolla, Greice. "Construção e seleção de uma biblioteca combinatorial de anticorpos contra herpesvirus bovino tipo 1." Universidade Federal de Goiás, 2014. http://repositorio.bc.ufg.br/tede/handle/tede/4215.
Повний текст джерелаApproved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-03-04T11:45:39Z (GMT) No. of bitstreams: 2 Dissertação - Greice Japolla - 2014.pdf: 735093 bytes, checksum: 4795f79b674744556e9029a8e9b99c2f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)
Made available in DSpace on 2015-03-04T11:45:39Z (GMT). No. of bitstreams: 2 Dissertação - Greice Japolla - 2014.pdf: 735093 bytes, checksum: 4795f79b674744556e9029a8e9b99c2f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-02-11
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Bovine herpesvirus type 1 ( BHV - 1 ) is recognized as an important pathogen of economic losses in cattle , these animals causing diseases known as infectious bovine rhinotracheitis ( IBR ) , infectious pustular vulvovaginitis , infectious balanoposthitis and neurological disorders . For effective control of these diseases, the correct diagnosis is necessary, but none of the available tests enables a quick result made the field. Considering this, the aim of this study was to construct a combinatorial antibody library, for it to be used in the future development of new diagnostic approaches . Two breed White Leghorn chickens were immunized with 105.5 TCID50/ml of BoHV - 1, birds were necropsied , their spleens removed for total RNA extraction , cDNA synthesis , amplification of gene fragments encoding the light chain ( VL ) and heavy ( VH ) and production of scFv ( v) fragments. These fragments were cloned into vectors fagomidiais expressed as fusion proteins on filamentous phage and amplified by infection of E. coli. Selection of viral particles ( fused scFv) binding to the BHV -1 ( biopanning ) by six cycles were performed. The affinity of the scFv antibody library BHV -1 observed in ELISA shows that the produced fragments are reactive to HIV, the use of such antibodies in the development of new diagnostic platforms is possible . The sequencing results showed a reduction of variability in comparison to the dot blot previously performed, and a desirable feature of this process , however, it was possible to sequence the clones efficiently, it has been found , therefore, a need to further analyze the shape of results
O herpesvírus bovino tipo 1 (BoHV-1) é reconhecido como um importante patógeno de perdas econômicas em bovinos, causando nestes animais enfermidades conhecidas como Rinotraqueite Infecciosa Bovina (IBR), vulvovaginite pustular infecciosa, balanopostite infecciosa e desordens neurológicas. Para um efetivo controle destas enfermidades, o diagnóstico correto se faz necessário, porém nenhuma dos testes disponíveis possibilita um resultado rápido feito a campo. Considerando isto, o objetivo deste estudo foi construir uma biblioteca combinatorial de anticorpos, para que esta seja futuramente utilizada no desenvolvimento de novas abordagens diagnósticas. Duas galinhas da raça White Leghorn foram imunizadas com 105,5 DICC50/mL de BoHV-1, as aves foram necropsiadas, seus baços retirados para extração de RNA total, síntese de cDNA, amplificação dos fragmentos gênicos codificantes das cadeias leve (VL) e pesada (VH) e produção de fragmentos scF(v). Estes fragmentos foram clonados em vetores fagomidiais, expressos como proteínas de fusão em bacteriófagos filamentosos e amplificados pela infecção de bactérias E.coli. Foi realizada a seleção de partículas virais (scFv fusionados) ligantes ao BoHV-1 (biopanning) através de seis ciclos. A afinidade da biblioteca de anticorpos scFv ao BoHV-1 observada no teste de ELISA mostra que os fragmentos produzidos são reativos ao vírus, sendo possível a utilização destes anticorpos no desenvolvimento de novas plataformas de diagnóstico. Os resultados de sequenciamento mostraram uma diminuição da variabilidade em comparação ao dot blot realizado anteriormente, sendo uma característica desejável neste processo, porém não foi possível sequenciar os clones de modo eficiente, verificando-se, portanto, a necessidade de analisar de forma mais aprofundada os resultados obtidos .
Zebedee, Zoë Anna-Marie. "Identification of scFv reagents which recognise the human neural cell adhesion molecule expressed upon neuroblastoma cells." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390796.
Повний текст джерелаSaxena, Abhishek. "Construction of immune scFv M13 phage display library and isolation of anti-glycan monoclonal antibodies." Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203295.
Повний текст джерелаLamort, Anne-Sophie. "Etude fonctionnelle de la MMp - 12 de macrophage en vue de son ciblage thérapeutique dans la broncho-pneumopathie chronique obstructive." Thesis, Tours, 2015. http://www.theses.fr/2015TOUR4043.
Повний текст джерелаChronic obstructive pulmonary disease or COPD is a lung disease caused by tobacco smoking. This is a chronic and non reversible disease for which no curative treatment is available yet. Permanent inflammation of the airways is a hallmark of COPD because immune cells such as neutrophils and macrophages are continuously recruited. Once activated, these cells release numerous active proteases which participate to the degradation of structural proteins of the lungs such as elastin, leading to lung emphysema as a consequence of lung alveoli degradation. Among the different proteases found in the lungs, macrophage MMP-12 has been reported to play a key pathogenic role in COPD development
Cloutier, Sylvain. "Sélection et production de scFv contre la kallicréine humaine hK2 par la technologie du phage display." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ33600.pdf.
Повний текст джерелаCampos, Lucas Benício. "Atividade tóxica da peçonha de Lachesis muta rhombeata e produção de fragmentos de anticorpos humanos (scFv) contra a peçonha bruta." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/17/17131/tde-21072016-110802/.
Повний текст джерелаThe current treatment for animal envenoming is the intravenous administration of antivenoms, produced by animal hyperimmunization. Unfortunately, available antivenoms sometimes do not protect patients and may cause hypersensitivity reactions. Phospholipases A2 (PLA2), L-amino acid oxidases, metallo and serine proteases are considered the most important snake venom components and contribute to neurotoxicity, hemorrhage, hemolysis, myotoxicity, edematogeny and cardiotoxicity. Assays for evaluating the enzymes present in Lachesis muta rhombeata venom were developed and the protease one was optimized. Phage display technology was used to select phage antibodies able to recognize the crude venom. Phages were amplified in Escherichia coli TG1 and used to infect E. coli HB2151, which produces human antibody fragments. Inhibition tests aiming the neutralization of some toxic components of the venom were performed using purified antibody fragments. Activity assays for evaluating PLA2, protease and L-aminoacid oxidase were successfully performed and all enzymes showed high activity levels. The molecular mass of PLA2 was estimated in 17 kDa and the molecular mass of proteases were estimated in 40, 35 and 24 kDa, by zymography. After optimizing the conditions for proteolytic assay, it was possible to use 25 times less venom than it was necessary at first. The bio panning method was efficient for selecting specific phage antibodies against the crude venom. Several clones were selected to infect HB2151 and to produce soluble antibody fragments, which were purified and incubated with the venom to test their inhibition capacity over each enzyme. Five clones demonstrated ability to neutralize PLA2 by inhibiting hemolysis. The clone 4E could inhibit 100% of hemolysis for over 2 hours when preincubated at the ratio 2:1 (scFv:venom). Clones 2C and 4E could inhibit 100% for 1 hour when preincubated at the ratio 1:1 and clones 2F e 9F could inhibit partially. Other tests will be performed to select clones able to neutralize other enzymes and, together with the clones already selected, will be evaluated by in vivo experiments. It is expected that they may contribute to the construction of a potential new antivenom able to overcome some of the problems associated with conventional immunotherapy
Figueiredo, Andreza Soriano [UNESP]. "Clonagem e expressão de fragmentos de anticorpos (scFV) contra o vírus da leucemia felina (FeLV) por phage display." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/106027.
Повний текст джерелаFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O vírus da leucemia felina (FeLV) é um retrovírus que infecta principalmente gatos jovens. Em aglomerados de animais, a infecção pelo FeLV é a que mais contribui para a mortalidade. O emprego de técnicas moleculares de detecção viral permitiu avanços no que diz respeito à caracterização da patogenia e resposta à vacinação. Baseando-se nesses novos resultados, o diagnóstico da infecção deve ser realizado, primeiramente, com um teste de triagem de detecção da proteína de capsídeo p27, e, posteriormente, a confirmação com teste de detecção de DNA proviral. O diagnóstico e separação de animais positivos constituem o mecanismo primordial para conter a disseminação do FeLV. Diante disso, é de grande importância facilitar o acesso e baratear o diagnóstico. A construção de um teste de detecção da p27 baseia-se na produção de anticorpos monoclonais. A técnica de hibridomas é menos prática e demanda mais tempo para a obtenção de resultados satisfatórios quando comparada à técnica de Phage Display. Esta está em franco desenvolvimento e tem ganhado grande aplicabilidade na medicina veterinária. Empregamos o sistema de Phage Display desenvolvido por Krebber e colaboradores (1997). Primeiramente, foi construída uma biblioteca imune em camundongos, em seguida, foram amplificadas as regiões gênicas variáveis das cadeias leve (VL) e pesada (VH) e ligadas com um Linker de (Gli4Ser)4. Esses fragmentos geneticamente construídos derivados de anticorpos são denominados de single chain variable fragment ou scFv. Os scFvs foram fusionados à pIII e apresentados na superfície de fagos filamentos. Após três ciclos de seleção e enriquecimento contra a p27 recombinante produzida no laboratório, onze scFvs foram selecionados e caracterizados com relação à constituição nucleotídica e de aminoácidos. Dentre eles, o scFv 9 e scFv 70 foram escolhidos...
The feline leukemia virus (FeLV) is a retrovirus that infects primarily young cats. In animal clusters, FeLV infection is the largest contributor to mortality. The use of molecular techniques for viral detection has allowed advances with regard to the pathogenicity and response to vaccination. Based on these new findings, the diagnosis of infection should be performed first, with a screening test for detection of p27 capsid protein, and subsequently confirmed with testing for proviral DNA. The diagnosis and segregation of positive animals is the primary mechanism to contain the FeLV spread. Therefore, it is of great importance to facilitate access and lower the diagnosis. The construction of a test for p27 detection relies on monoclonal antibody development. The hybridoma technique is less practical and more time consuming to obtain satisfactory results when compared to Phage Display technology. The latter has been improved rapidly and has gained wide application in veterinary medicine. We employed the Phage Display system developed by Krebber et al. (1997). First, an immune library was built in mice and the variable region of the light and the heavy genes were amplified and connected by a linker of (Gly4Ser)4. This genetically engineered antibody fragments are called single chain variable fragments or scFv. The scFvs were fused to the pIII protein and displayed on the surface of filamentous phages. After three rounds of selection and enrichment against recombinant p27 (produced in the laboratory), eleven scFvs were selected and characterized with respect to nucleotide and aminoacid composition. Among them, scFv 9 and scFv 70 were chosen for subcloning and expression in prokaryotic system for production of heterologous proteins. The scFvs in soluble forms were evaluated for their binding capacity to p27. The scFvs will be employed to the development of an immunoassay for FeLV detect... (Complete abstract click electronic access below)
Книги з теми "Phage displayed scFv"
Nguyen, Hai Phu. Combination of hu-PBL-SCID mice and scFv phage display library: An effective alternative for hu-mAb production. 2001.
Знайти повний текст джерелаЧастини книг з теми "Phage displayed scFv"
Brockmann, Eeva-Christine. "Selection of Stable scFv Antibodies by Phage Display." In Antibody Engineering, 123–44. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-974-7_7.
Повний текст джерелаDamasceno, Leonardo M., Frank Lee, Gerd Ritter, Lloyd Old, and Carl Batt. "High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris." In Methods in Molecular Biology, 225–36. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-302-2_18.
Повний текст джерелаSingh, Pawan Kumar, Ranu Agrawal, D. V. Kamboj, and Lokendra Singh. "Construction of Recombinant Single Chain Variable Fragment (ScFv) Antibody Against Superantigen for Immunodetection Using Antibody Phage Display Technology." In Superantigens, 207–25. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-3344-0_17.
Повний текст джерелаNahary, Limor, Alla Trahtenherts, and Itai Benhar. "Isolation of scFvs that Inhibit the NS3 Protease of Hepatitis C Virus by a Combination of Phage Display and a Bacterial Genetic Screen." In Methods in Molecular Biology, 115–32. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-302-2_9.
Повний текст джерелаТези доповідей конференцій з теми "Phage displayed scFv"
Pruksametanan, Natcha, Montarop Yamabhai, and Pakamatz Khawplod. "Selection of single chain human monoclonal antibody (scFv) against Rabies virus by phage display technology." In 2012 IEEE 6th International Conference on Nano/Molecular Medicine and Engnieering (NANOMED). IEEE, 2012. http://dx.doi.org/10.1109/nanomed.2012.6509127.
Повний текст джерелаBezerra, Marcus, Andrea Maranhao, Igor Studart, Larissa Pontes, Marcela Fonseca, and Gilvan Furtado. "Construction of a scFv library using directed evolution for rituximab-based therapies: using phage display towards antibody affinity maturation." In IV International Symposium on Immunobiologicals & VII Seminário Anual Científico e Tecnológico. Instituto de Tecnologia em Imunobiológicos, 2019. http://dx.doi.org/10.35259/isi.sact.2019_32708.
Повний текст джерелаFigueiredo, Alexandre, Thiago Chaves, Fernando Conte, Rodrigo Silva, Milena Carvalho, Manoela Martins, Adriana Soares, Sheila Lima, and Patrícia Neves. "Development of Single-Chain Variable Fragment (ScFv) antibody against COVID-19 by phage display as a possible tool to diagnostic and treatment." In International Symposium on Immunobiological. Instituto de Tecnologia em Imunobiológicos, 2021. http://dx.doi.org/10.35259/isi.2021_46572.
Повний текст джерелаAhmed, Kafil, Vyankatesh Pidiyar, Syed Ahmed, and Sanket Shah. "Development of anti-SARS-CoV-2 specific scFv antibody library from convalescent plasma of COVID-19 recovered patients using phage display technology." In International Symposium on Immunobiological. Instituto de Tecnologia em Imunobiológicos, 2021. http://dx.doi.org/10.35259/isi.2021_46568.
Повний текст джерела