Добірка наукової літератури з теми "Pathogenic potential of V.parahaemolyticu"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Pathogenic potential of V.parahaemolyticu".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Статті в журналах з теми "Pathogenic potential of V.parahaemolyticu"
1
Gleire Rodrigues de Menezes, Francisca, Soraya Silva Neves, Rafael Dos Santos Rocha, Oscarina Viana Sousa, Marina Teresa Rodriguez Torres, Rodrigo Maggioni, Ernesto Hofer, and Regine Helena Silva dos Fernandes Vieira. "PATHOGENIC POTENTIAL OF Vibrio parahaemolyticus ISOLATED FROM TROPICAL ESTUARINE ENVIRONMENTS IN CEARÁ, BRAZIL." Revista de Patologia Tropical / Journal of Tropical Pathology 49, no. 2 (July 13, 2020): 94–104. http://dx.doi.org/10.5216/rpt.v49i2.61338.
Повний текст джерелаАнотація:
Vibrio parahaemolyticus is a potentially pathogenic bacterium that occurs naturally in estuarine environments worldwide. This research aimed to investigate the occurrence of Vibrio parahaemolyticus in estuarine environments and determine the virulence profile in aquaculture environment by molecular techniques and conventional microbiological methods. The analysis included 64 samples of water (n=32) and sediment (n=32) collected in estuaries. The samples yielded 64 strains suspected to be V. parahaemolyticus. The isolates were submitted to biochemical identification using a dichotomous key and PCR for the detection of the species-specific gene tl. Virulence was assessed by testing for urea hydrolysis and ?-hemolysis in erythrocytes (Kanagawa phenomenon) and simultaneous detection of the genes tdh and trh. All but one of the isolates (63/64) were confirmed to be V. parahaemolyticus by genotypic detection of tl. The genes tdh and trh were detected in 57 and 19 isolates, respectively. The Kanagawa test was positive for 51 isolates. Only one isolate was positive for urease. The incidence of tdh/trh-positivity was very high in strains isolated from the environment. The present study demonstrates that the necessity to improve the knowledge of the ecology and pathogeny of V. parahaemolyticus.
2
Almuhaideb, Esam, Lathadevi K. Chintapenta, Amanda Abbott, Salina Parveen, and Gulnihal Ozbay. "Assessment of Vibrio parahaemolyticus levels in oysters (Crassostrea virginica) and seawater in Delaware Bay in relation to environmental conditions and the prevalence of molecular markers to identify pathogenic Vibrio parahaemolyticus strains." PLOS ONE 15, no. 12 (December 3, 2020): e0242229. http://dx.doi.org/10.1371/journal.pone.0242229.
Повний текст джерелаАнотація:
This study identified Vibrio parahaemolyticus in oyster and seawater samples collected from Delaware Bay from June through October of 2016. Environmental parameters including water temperature, salinity, dissolved oxygen, pH, and chlorophyll a were measured per sampling event. Oysters homogenate and seawater samples were 10-fold serially diluted and directly plated on CHROMagarᵀᴹ Vibrio medium. Presumptive V. parahaemolyticus colonies were counted and at least 20% of these colonies were selected for molecular chracterization. V. parahaemolyticus isolates (n = 165) were screened for the presence of the species-specific thermolabile hemolysin (tlh) gene, the pathogenic thermostable direct hemolysin (tdh)/ thermostable related hemolysin (trh) genes, the regulatory transmembrane DNA-binding gene (toxR), and V. parahaemolyticus metalloprotease (vpm) gene using a conventional PCR. The highest mean levels of the presumptive V. parahaemolyticus were 9.63×103 CFU/g and 1.85×103 CFU/mL in the oyster and seawater samples, respectively, during the month of July. V. parahaemolyticus levels in oyster and seawater samples were significantly positively correlated with water temperature. Of the 165 isolates, 137 (83%), 110 (66.7%), and 108 (65%) were tlh+, vpm+, and toxR+, respectively. Among the V. parahaemolyticus (tlh+) isolates, 7 (5.1%) and 15 (10.9%) were tdh+ and trh+, respectively, and 24 (17.5%), only oyster isolates, were positive for both genes. Potential pathogenic strains that possessed tdh and/or trh were notably higher in oyster (39%) than seawater (15.6%) isolates. The occurrence of total V. parahaemolyticus (tlh+) was not necessarily proportional to the potential pathogenic V. parahaemolyticus. Co-occurrence of the five genetic markers were observed only among oyster isolates. The co-occurrence of the gene markers showed a relatedness potential of tdh occurrence with vpm. We believe exploring the role of V. parahaemolyticus metalloprotease and whether it is involved in the toxic activity of the thermostable direct hemolysin (TDH) protein can be of significance. The outcomes of this study will provide some foundation for future studies regarding pathogenic Vibrio dynamics in relation to environmental quality.
3
Novotny, L., L. Dvorska, A. Lorencova, V. Beran, and I. Pavlik. "Fish: a potential source of bacterial pathogens for human beings ." Veterinární Medicína 49, No. 9 (March 29, 2012): 343–58. http://dx.doi.org/10.17221/5715-vetmed.
Повний текст джерелаАнотація:
Human infections caused by pathogens transmitted from fish or the aquatic environment are quite common and depend on the season, patients’ contact with fish and related environment, dietary habits and the immune system status of the exposed individual. They are often bacterial species facultatively pathogenic for both fish and human beings and may be isolated from fish without apparent symptoms of the disease. The infection source may be fish kept for both for food and as a hobby. Human infections and intoxications with the following bacteria have been recorded: Mycobacterium spp., Streptococcus iniae, Photobacterium damselae, Vibrio alginolyticus, V. vulnificus, V. parahaemolyticus, V. cholerae, Erysipelothrix rhusiopathiae, Escherichia coli, Aeromonas spp., Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, Clostridium botulinum, C. perfringens, Campylobacter jejuni, Delftia acidovorans, Edwardsiella tarda, Legionella pneumophila, and Plesiomonas shigelloides. Fish tissue histamine intoxications of people have frequently been described. The purpose of the present paper was to elaborate an overview of significant bacterial causative agents of human diseases transmitted from fish used as food or by handling them.
4
Kohli, Vandita, Ramasubramanian Vaidhyanathan, Amjad K. Balange, Binaya Bhusan Nayak, and Sanath H. Kumar. "Distribution of Vibrio parahaemolyticus in Farmed Shrimp Penaeus vannamei, Farm Water and Sediment." Journal of Pure and Applied Microbiology 15, no. 3 (August 23, 2021): 1608–16. http://dx.doi.org/10.22207/jpam.15.3.57.
Повний текст джерелаАнотація:
The halophilic marine bacterium Vibrio parahaemolyticus is a zoonotic pathogen associated with wild-caught and farmed shrimp. The bacterium is an important cause of gastroenteritis associated with the consumption of raw or undercooked seafood. In the present study, the prevalence and human pathogenic potential of Vibrio parahaemolyticus in Penaeus vannamei (tissue and hepatopancreas) and the farm environment (water and sediment) was investigated by conventional culture and molecular techniques. The total Vibrio counts of P. vannamei ranged from <1 CFU/mL in hemolymph to 7.61 log CFU/g in the hepatopancreas. The sediment samples consistently showed the counts of 6-7 log CFU/g, while the pond water had Vibrio counts in the range of 2-3 log CFU/ml. Of 120 Vibrio isolates identified, 87 were confirmed as V. parahaemolyticus based on the toxR and tlh gene-specific PCR. The virulence marker gene tdh was not detected in any of the isolates, while the trh gene was detected in 3 (3.6%) isolates. Although the incidence of pathogenic V. parahaemolyticus in farmed P. vannamei is low, the high numbers of total vibrios and V. parahaemolyticus demand constant monitoring of animals and the farm environment for human pathogenic strains of V. parahaemolyticus.
5
KAYSNER, CHARLES A., CARLOS ABEYTA, ROBERT F. STOTT, MARY HELEN KRANE, and MARLEEN M. WEKELL. "Enumeration of Vibrio Species, Including V. cholerae, from Samples of an Oyster Growing Area, Grays Harbor, Washington." Journal of Food Protection 53, no. 4 (April 1, 1990): 300–301. http://dx.doi.org/10.4315/0362-028x-53.4.300.
Повний текст джерелаАнотація:
Water, shellfish, and sediment samples from Grays Harbor, a major commercial oyster producing estuary in the State of Washington, were examined for levels of Vibrio species. Non-01 V. cholerae was found at low levels in 37.8% of the samples. While V. parahaemolyticus was found in all samples, levels were low. V. mimicus and V. fluvialis were found infrequently and at low levels. Potentially pathogenic strains of non-01 V. cholerae and Kanagawa positive V. parahaemolyticus were isolated from oysters suggesting a potential for human illness.
6
Palamae, Suriya, Ajay Mittal, Mingkwan Yingkajorn, Jirakrit Saetang, Jirayu Buatong, Anuj Tyagi, Prabjeet Singh, and Soottawat Benjakul. "Vibrio parahaemolyticus Isolates from Asian Green Mussel: Molecular Characteristics, Virulence and Their Inhibition by Chitooligosaccharide-Tea Polyphenol Conjugates." Foods 11, no. 24 (December 14, 2022): 4048. http://dx.doi.org/10.3390/foods11244048.
Повний текст джерелаАнотація:
Fifty isolates of Vibrio parahaemolyticus were tested for pathogenicity, biofilm formation, motility, and antibiotic resistance. Antimicrobial activity of chitooligosaccharide (COS)-tea polyphenol conjugates against all isolates was also studied. Forty-three isolates were randomly selected from 520 isolates from Asian green mussel (Perna viridis) grown on CHROMagarTM Vibrio agar plate. Six isolates were acquired from stool specimens of diarrhea patients. One laboratory strain was V. parahaemolyticus PSU.SCB.16S.14. Among all isolates tested, 12% of V. parahaemolyticus carried the tdh+trh– gene and were positive toward Kanagawa phenomenon test. All of V. parahaemolyticus isolates could produce biofilm and showed relatively strong motile ability. When COS-catechin conjugate (COS-CAT) and COS-epigallocatechin-3-gallate conjugate (COS-EGCG) were examined for their inhibitory effect against V. parahaemolyticus, the former showed the higher bactericidal activity with the MBC value of 1.024 mg/mL against both pathogenic and non-pathogenic strains. Most of the representative Asian green mussel V. parahaemolyticus isolates exhibited high sensitivity to all antibiotics, whereas one isolate showed the intermediate resistance to cefuroxime. However, the representative clinical isolates were highly resistant to nine types of antibiotics and had multiple antibiotic resistance (MAR) index of 0.64. Thus, COS-CAT could be used as potential antimicrobial agent for controlling V. parahaemolyticus-causing disease in Asian green mussel.
7
Thongchankaew, Uraiwan, Pimonsri Mittraparp-arthorn, Pharanai Sukhumungoon, Natta Tansila, Taiyeebah Nuidate, Mitsuaki Nishibuchi, and Varaporn Vuddhakul. "Occurrence of potentially pathogenic vibrios and related environmental factors in Songkhla Lake, Thailand." Canadian Journal of Microbiology 57, no. 11 (November 2011): 867–73. http://dx.doi.org/10.1139/w11-084.
Повний текст джерелаАнотація:
Vibrios are halophilic bacteria that are ubiquitous in marine environments. Their occurrence in tropical lakes has rarely been investigated. In this study, the predominance and diversity of Vibrio spp. was investigated over a 12-month period in a coastal lagoon, Songkhla Lake, in southern Thailand. Water samples were collected at 2 stations in the estuary near Yor Island in Songkhla Lake. The predominant vibrios were detected by a culture-based method, using thiosulfate–citrate–bile salt–sucrose agar and CHROMagar Vibrio. The diversity of Vibrio spp. was evaluated using denaturant density gradient electrophoresis (DGGE). The highest numbers of total vibrios and Vibrio parahaemolyticus in both areas were observed during the summer. There was no significant correlation between the numbers of vibrios, including V. parahaemolyticus, and either the water temperature or plankton density. Variations in Vibrio species were observed with changes in salinity. Vibrio parahaemolyticus and V. cholerae non-O1/non-O139 were detected during the rainy season when the salinity dropped to nearly 0 parts per thousand. In both areas, V. alginolyticus was the most prominent species detected by the culture method, whereas Vibrio parahaemolyticus was detected by DGGE, every month. Other Vibrio spp. of potential public health concern were also detected by the culture method; they included V. vulnificus , V. fluvialis , and V. mimicus .
8
MATTÉ, GLAVUR R., MARIA H. MATTÉ, IRMA G. RIVERA, and MARIA T. MARTINS. "Distribution of Potentially Pathogenic Vibrios in Oysters from a Tropical Region." Journal of Food Protection 57, no. 10 (October 1, 1994): 870–73. http://dx.doi.org/10.4315/0362-028x-57.10.870.
Повний текст джерелаАнотація:
Oysters (Crassostrea gigas) originating from the southern coast of the State of São Paulo-Brazil were analyzed for Vibrio species. Most Probable Numbers (MPN/100 g) were obtained for Vibrio alginolyticus (<3-1,500), Vibrio parahaemolyticus (<3-1,200), Vibrio fluvialis (<3-150), Vibrio cholerae non 01 (<3-40), Vibrio furnissii (<3-40), Vibrio mimicus (<3-40) and Vibrio vulnificus (<3-30). The highest incidence was observed for V. alginolyticus (81%), followed by V. parahaemolyticus (77%), V. cholerae non 01 (31 %), V. fluvialis (27%), V. furnissii (19%), V. mimicus (12%), and V. vulnificus (12%). Forty-eight percent of the isolates tested were positive for enterotoxins in the rabbit ileal loop (RIL) test and 11.1% in the suckling mice test. Vibrio parahaemolyticus (1.1 %) was positive in the Kanagawa test. Vibrio vulnificus (25%) showed lethality in young adult mice. During the field inspection it was observed that generally the conditions of storage were not adequate as 65% of the samples were maintained in temperatures ranging from 25 to 40°C, 19% were frozen, 12% refrigerated and 4% was immersed in contaminated seawater. These results emphasize the great potential for food poisoning by inadequately preserved seafood, and the necessity to upgrade the standards for food quality assessment.
9
WAGLEY, SARIQA, KEGAKILWE KOOFHETHILE, and RACHEL RANGDALE. "Prevalence and Potential Pathogenicity of Vibrio parahaemolyticus in Chinese Mitten Crabs (Eriocheir sinensis) Harvested from the River Thames Estuary, England." Journal of Food Protection 72, no. 1 (January 1, 2009): 60–66. http://dx.doi.org/10.4315/0362-028x-72.1.60.
Повний текст джерелаАнотація:
Chinese mitten crabs (Eriocheir sinensis) have been described as an alien invasive species in the River Thames, United Kingdom, and elsewhere in Europe. The crabs can cause considerable physical damage to the riverbeds and threaten native ecosystems. Trapping has been considered an option, but such attempts to control mitten crab populations in Germany in the 1930s failed. In the United Kingdom, it has been suggested that commercial exploitation of the species could be employed as a control option. This study was conducted as part of a larger program to assess the suitability of a commercial Chinese mitten crab fishery in the River Thames. Crabs and water samples from the River Thames between 2003 and 2006 were examined for the human pathogenic bacterium Vibrio parahaemolyticus. All samples throughout this testing period were positive for V. parahaemolyticus. The putative pathogenicity markers, thermostable direct hemolysin and thermostable direct–related hemolysin, were detected in one sample, indicating that the crabs possessed the potential to cause V. parahaemolyticus–associated illness if consumed without further processing. Levels of V. parahaemolyticus were higher during the summer than in the winter. This is the first study of V. parahaemolyticus prevalence in European-adapted Chinese mitten crabs.
10
Ilmiah, Sukenda, Widanarni, and Enang Harris. "Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus." Jurnal Akuakultur Indonesia 11, no. 1 (November 14, 2013): 28. http://dx.doi.org/10.19027/jai.11.28-37.
Повний текст джерелаАнотація:
<p>This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of <em>Vibrio</em> was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 10<sup>6</sup> CFU/fish and mortality of fish during seven days observation then was noted. Then, the <em>Vibrio</em> pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as <em>V</em><em>ibrio</em><em> metschnikovii</em>,<em> V</em><em>.</em><em> parahaemolyticus</em>, and <em>V. mimicus</em>. The most virulent among isolates was <em>V. parahaemolyticus.</em></p> <p>Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper</p>
Дисертації з теми "Pathogenic potential of V.parahaemolyticu"
1
Lorencová, Markéta. "Diverzita rodu Blastocystis (Stramenopiles) v plazech a členovcích." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-336900.
Повний текст джерелаАнотація:
The genus Blastocystis has recently attracted the attention of scientists, especially parasitologists. Similarly to the related opalines and proteromonads, Blastocystis is anaerobic and lives endobiotically in the intestine of various animals. This organism is also often found in humans, where it is associated with irritable bowel syndrome, though its pathogenic potential remains uncertain. The genus Blastocystis is remarkable for its rich genetic diversity. The taxonomy of Blastocystis is inconsistent and problematic. The strains isolated from homoiothermic vertebrates are divided into 17 subtypes, while strains from poikilotherms are either classified as separate species or are not considered in taxonomic studies at all. The aim of the study was to further examine the genetic diversity of the genus Blastocystis. We determined SSU rDNA sequences of 38 strains isolated from poikilothermic vertebrates and arthropods. The results of our phylogenetic analysis showed that Blastocystis is considerably diverse in these hosts, and we defined 21 new subtypes. The total number of known subtypes of Blastocystis has thus increased to 38. We also examined light-microscopical morphology of some strains. Most of the newly defined subtypes show identical morphology, ST20 (Blastocystis geocheloni) is an exception,...
Частини книг з теми "Pathogenic potential of V.parahaemolyticu"
1
Chellapandian, Hethesh, Jeyachandran Sivakamavalli, A. Vijay Anand, and Balamuralikrishnan Balasubramanian. "Challenges in Controlling Vibriosis in Shrimp Farms." In Infectious Diseases and Sepsis [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97018.
Повний текст джерелаАнотація:
Recently the shrimp farming has blooming as a crucial counterpart in the aquaculture industry which contribute the remarkable role in sea food production as well economy of the country. However, this could be fluctuated every year through several circumstances such as unfavorable (Poor water and soil quality) environmental factors. The environmental factors includes disease causing bacterial pathogens in the soil and water which causes the bacterial diseases in the aquatic animals, like this hectic problems are prevented through bioaugmentation strategies. The pond environment plays a vital role in determining the healthy culture system, but there is high risk for manipulation by bacterial community which takes care of waste generated in the system through in situ bioremediation. Due to the impact of rapidly growing bacterial diseases of shrimps throughout the world, numerous studies have been carried out to find immunostimulants, immunomodulators and biotic component that can be used against vibrio causing pathogens, and can also be used as an alternative for antibiotics. Recent research focus towards the marine resources such as microalgae, seaweed, live feeds (like artemia, copepods, rotifers), bacteriophage, and probiotics have been found to have higher potential in reducing vibriosis. Eco-based shrimp farming includes green water technology, phage therapy bio-floc technology (BFT) and integrated multi-trophic aquaculture (IMTA), these methods hold a promising alternative to antibiotics in the near future. Bacterial diseases caused by vibrios have been reported in penaeid shrimp culture systems implicating at least 14 species and they are Vibrio harveyi, V. splendidus, V. parahaemolyticus, V. alginolyticus, V. anguillarum, V. vulnificuslogei etc.
Тези доповідей конференцій з теми "Pathogenic potential of V.parahaemolyticu"
1
Cherepanova, M. A., G. V. Mitina, and A. A. Choglokova. "PATHOGENIC POTENTIAL OF ENTOMOPATHOGENIC FUNGI OF THE GENUS LECANICILLIUM AGAINST HEMIPTERA." In V International Scientific Conference CONCEPTUAL AND APPLIED ASPECTS OF INVERTEBRATE SCIENTIFIC RESEARCH AND BIOLOGICAL EDUCATION. Tomsk State University Press, 2020. http://dx.doi.org/10.17223/978-5-94621-931-0-2020-70.
Повний текст джерелаАнотація:
The virulence of the species of the entomopathogenic fungi Lecanicillium psalliotae (strain Vl 78), L. dimorphum (Vl 79) and L. pissodis (ARSEF 8057) against bean aphid Megoura viciae Buckt and greenhouse whitefly Trialeurodes vaporariorum Westwood has been compared with the virulence of well-studied species L. muscarium (Vl 21, Vl 72), L. lecanii (Vl 5), L. longisporum (Vl 13). Species L. longisporum (Vl 13), L. muscarium (Vl 72), L. psalliotae (Vl 78) had a virulence of more than 80% against bean aphid, other strains showed the virulence less than 60%. All strains except L. longisporum (Vl 13) had a high virulence about 75–100% against the greenhouse whitefly. As appeared, the species L. psalliotae (Vl 78) has the perspectives against the both insect species. Its virulence corresponds to the similar level of mortality caused by the well-studied species L. muscarium.
2
Gao, Yali, Philip M. Sherman, Yu Sun, and Dongqing Li. "Multiplexed High-Throughput Electrokinetically-Controlled Immunoassay on a Chip for the Detection of Specific Bacterial Antibodies in Human Serum." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-42512.
Повний текст джерелаАнотація:
This work presents a multiplexed electrokinetically-controlled heterogeneous immunoassay that can process ten samples in parallel. The immunoassay microchip was soft-lithographically fabricated using poly(dimethylsiloxane) and glass. Controlling parameters of the electrokinetically-driven flow in the microfluidic network was determined by numerically simulating transport processes. Multiple passively adsorbed antigens captured antibodies present in samples, which then bound with TRITC-labeled detection antibodies to generate fluorescent signals. Antibodies against Escherichia coli O157:H7 and Helicobacter pylori were studied as model analytes. After conditions for antigen-coating were optimized, a 24-minute assay detected E. coli O157:H7 antibody in the concentration range of 0.02–10 μg/mL, and H. pylori antibody in the range of 0.1–50 μg/mL. In testing human serum samples, non-specific binding of serum components was effectively suppressed by using 10% (w/v) bovine serum albumin. An accuracy of 100% was achieved in detecting either E. coli O157:H7 antibody or H. pylori antibody from human serum samples. Simultaneous screening of both antibodies was also successfully demonstrated. The immunoassay chip shows an excellent potential for efficiently detecting multiple pathogenic infections in clinical environments.
Звіти організацій з теми "Pathogenic potential of V.parahaemolyticu"
1
Rowe, Randall C., Jaacov Katan, Talma Katan, and Leah Tsror. Sub-Specific Populations of Verticillium dahliae and their Roles in Vascular Wilt Pathogsystems. United States Department of Agriculture, October 1996. http://dx.doi.org/10.32747/1996.7574343.bard.
Повний текст джерелаАнотація:
Verticillium dahliae is an economically important pathogen causing vascular wilt on over 160 plant species. In North America, potato early dying is a significant disease of potato, especially in the midwest and Pacific northwest states. This disease is caused by the fungus Verticillium dahliae and in some cases involves a synergistic interaction with root-lesion nematodes, primarily Pratylenchus penetrans. In Israel, Verticillium wilt occurs in many regions and inflicts serious losses in potato, cotton, and other crops. Objectives of this project were to establish a large collection of isolates of Verticillium dahliae from potato (USA) and several host plants (Israel) and to characterize and compare the isolates with regard to morphology, vegetative compatibility group (VCG), and pathogenic capabilities on several hosts. Isolations were made from 224 commercial lots of certified potato seed tubers from across N. America and 87 potato fields located in the Columbia Basin of Oregon and Washington. A large collection of isolates from central U.S. states already existed. In Israel, 47 field sites were sampled and isolates of Verticillium dahliae were recovered from 13 host plant species and from soil. Potato isolates from N. America were tested for vegetative compatibility and all found to be in VCG 4 with about 2/3 in VCG 4A and the rest in VCG 4B. VCG 4A isolates were significantly more aggressive on potato than VCG 4B isolates and were more likely to interact synergistically with P. penetrans. The Israeli isolates fell into three vegetative compatibility groups. Nearly all (> 90%) VCG2B and VCG 4B isolates were recovered from the northern and southern parts of Israel, respectively, with some overlap in central areas. Several pathotypes were defined in cotton, using cotton and eggplant together as differentials. All VCG 2B isolates from cotton caused severe disease in cotton, while VCG 2A and VCG 4B isolates from several crops were much less aggressive to cotton. When Israeli isolates of VCGs 2A, 2B and 4B were inoculated to potato and tomato, VCG 4B isolates caused much more severe disease on potato and VCG 2A isolates caused much more severe disease in tomato. Differential patterns of pathogenicity and aggressiveness of these VCGs on potato and tomato were consistent regardless of the host plant of origin. Isolates of the same VCG resembled one another more than isolates from different VCGs based on colony and microsclerotial morphology, temperature responses and, partially, in pathogenicity. Vegetative compatibility grouping of V. dahliae in Israel appears closely associated with specific pathogenicity and other phenotypic traits. The absence of VCG 4A in Israel is significant. VCG patterns among Verficillium populations are useful to predict relatedness and pathogenic potential in both countries.