Дисертації з теми "Papillomavirus humain (HPV)"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Papillomavirus humain (HPV)".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Olivera-Botello, Gustavo. "Modélisation numérique des aspects immunologiques de la réaction à l’infection à HPV et de la vaccination anti-HPV par Gardasil®." Thesis, Lyon 1, 2011. http://www.theses.fr/2011LYO10038/document.
Two prophylactic vaccines have demonstrated to prevent infections with the human papillomavirus (HPV). Thus, they have been in the market for the last four years, or so. The three main objectives of the present project were: i) to study in-silico the immunogenicity of one of these vaccines (Gardasil®); ii) to study in-silico the natural history of an HPV infection, and iii) to assess in-silico the potential of the following therapeutic hypothesis : the intramuscular administration of Gardasil® to patients already suffering from a recurrent respiratory papillomatosis would result in a better prognosis thanks to the fact that the HPV-specific immunoglobulins that would bathe the affected tissue would impede the virus to complete its life cycle and, therefore, the disease to progress. The main conclusions are: i) according to our simulations, the minimum serum IgG titer required for hampering the progression of a recurrent respiratory papillomatosis would be 200 mMU/mL ; ii) in order to keep, within a time window of ten years, the anti-HPV IgG titer over the just-mentioned therapeutic-effect threshold, the biggest possible fraction of time and through the administration of the smallest possible number of booster doses, it would be necessary, according to our simulations, to adopt the following vaccination schedule: the basic three doses (at months 0, 2 and 6), followed by three successive booster doses, every six months, until reaching the 24th month, followed by a late final booster dose, 18 months later. iii) incidentally, it would seem to be inappropriate, according to our simulations, to modify the original initial vaccination schedule (at months 0, 2 and 6)
Bernard, Xavier. "Stabilisation de p53 et rôle du kinome humain dans l'oncogenèse HPV." Strasbourg, 2010. http://www.theses.fr/2010STRA6223.
Third leading cause of cancer death in women, cervical carcinomas are associated in 99. 7% of cases with infection of human papillomavirus (HPV types 16 and 18 mostly). The oncogenic effect of these HPV is mainly caused by the integration of genes encoding two viral oncoproteins, E6 and E7 into the genome of infected cells. Specific inhibition of the E6 oncoprotein is a major therapeutic challenge. Indeed, inhibition of E6 leads to a restoration of p53 protein and induces the orientation of the cancer cells to senescence or apoptosis. However, the development of new therapeutic strategies requires an understanding of the carcinogenesis mechanisms in order to discover new targets and enable the development of effective strategies. It is within this scientific context that I entered my thesis project based on the molecular mechanisms dissection of the p53 protein degradation mediated by E6. For that, we articulated our work around two lines of research: (i) determination of the residues of p53 involved in the binding site with E6 of "high risk" HPV, responsible for the degradation of p53; (ii) identification of kinases involve in the p53 degradation in HPV transformed cells. This work demonstrated the importance of the conformation of the p53 core domain in the binding with the oncoprotein E6, by the structure / function studies of mutant p53. In addition, we have highlighted the role of the pseudo-kinase NRBP1 in the regulation of p53 in HPV transformed cells, via the NF-kB pathway
Mourareau, Céline. "Bio-CAD - Etude de biomarqueurs de progression tumorale dans les cancers des voies aéro-digestives supérieures en fonction de leur statut HPV." Thesis, Reims, 2016. http://www.theses.fr/2016REIMS029/document.
Each year, 610,000 cancers are diagnosed worldwide attributed to high risk human papillomavirus (HR-HPV) infection. Although head and neck squamous cell carcinoma (HNSCC) is mainly associated with tobacco and/or alcohol consumption, 20 to 25% are caused by HPV infection, particularly HPV type 16. Although patients with HPV+ tumors present a better overall survival, they are diagnosed with more lymph node metastasis than HPV-negative patients.Through a study of HNSCC derived cell lines, we showed that all HPV-positives cell lines harbored HPV genome integration through host genome, with different integration profiles. Cell lines identified as good HPV+ and HPV- tumors models are UPCI:SCC090 and FaDu respectively. The first one by its migratory and proliferative properties, the second through its poor aggressiveness and mutation of p53 cellular gene.In a study on a retrospective series of oropharyngeal carcinomas with surgical resection, 6 out of 40 cancers shown HPV16 active infection (expressing E6*I mRNA). We studied epithelial-to-mesenchymal transition (EMT) markers on this oropharyngeal cancers, according to HPV status. We found a larger loss of epithelial marker E-cadherin in HPV+ group and loss of this marker is associated with a worse overall survival.We showed that HPV and EMT status seem to be two independent factors that could combine differently to define different prognostic levels
Bonneault, Mélanie. "Modélisation dynamique des infections et co-infections génitales à papillomavirus humain (HPV) et de l’impact à long terme de la vaccination anti-HPV." Thesis, université Paris-Saclay, 2021. http://www.theses.fr/2021UPASR002.
Genital human papillomavirus (HPV) infection affects nearly one-third of people under the age of 25 years from the start of their sexual activity. Generally asymptomatic, it can lead to the development of cancerous lesions. Among the forty or so HPV genotypes transmitted via the genital tract, about fifteen have been evaluated as oncogenic and causal agents of cervical cancer. Two vaccines offered to young girls in France since 2007 target the two HPV genotypes most at risk of cervical cancer. As these vaccines only include a fraction of the HPV genotypes, the evolution of the prevalences of infection and co-infection remains uncertain. The aim of this thesis is to better understand the impact of interactions between HPV genotypes during intra-host co-infections on the evolution of the prevalences of vaccine (V) and non-vaccine (NV) genotypes. To meet this objective, this work is based on the development of an individual-based model that makes it possible to reproduce both the heterogeneity of sexual behaviour and the transmission dynamics of V and NV genotypes as functions of age. A first part of this thesis presents a detailed description of this stochastic model and its validation on survey data. This model assumes that the interaction between genotypes results in the reduction (competition) or extension (synergy) of the duration of infection by an NV genotype in the event of prior infection by a V genotype. Calibration of transmission parameters for various interaction strengths shows that several of them are compatible with pre-vaccine epidemiological data on infection and co-infection. In the simulations, after introduction of vaccination into the population, we observe that the prevalence of NV genotypes increases in the case of competition and decreases in the case of synergy, especially when the interaction is strong. In the event of competition, the increase in the prevalence of NV could lead to a slight decrease or even an increase in the overall prevalence of all genotypes despite vaccination. The second part aims to explore, through a simulation study, how the introduction of vaccination modifies the spread of infection in the contact network. The simulations highlight variations in NV prevalence before and after vaccination which are more marked in less active individuals. In the third part, the model is used to emulate epidemiological studies in order to determine the conditions (number of subjects, time after the introduction of the vaccine) necessary to detect a decrease or increase in HPV prevalences following vaccine introduction in the population. A systematic review of the literature reveals two observational study designs comparing the prevalences of infection either in two populations in the pre- and post-vaccination eras, or in vaccinated and unvaccinated people in the post-vaccination era. The results obtained suggest that the studies published to date, regardless of the design, lack statistical power to detect variation in NV prevalence. Based on the development of a model validated to reproduce realistic sexual behaviours and prevalences of HPV infection, this thesis work contributes to the improvement of epidemiological knowledge on HPV infections and co-infections and allows us to anticipate the impact of vaccine prevention measures on the prevalence of HPV infection
Fleury, Maxime Jean Jules. "Identification des épitopes inducteurs d'anticorps neutralisants de la protéine majeure de capside des papillomavirus humain de type 16 et 31." Tours, 2007. http://www.theses.fr/2007TOUR3309.
Human papillomaviruses (HPV) are the ethiologic agent of cervix cancer. Virus like particles (VLP) can be obteined by expressing capsid protein into eucaryotic systems. Those VLP have virions similar epitopes and can transfert reporter genes. The aim of this study was to identify the L1 protein epitopes of HPV 16 and 31 using monoclonal antibodies, L1 protein mutants, peptides sets, BIACORE and Bacterial display. Presence of HPV 16 major epitope and overlapping epitopes inducing neutralizing antibodies and cross-neutralizing antibodies was confirmed into the FG loop. The results show that the epitopes which induce neutralizing antibodies could be overlapping and on the FG loop of the HPV 31. Immunogenicless vector derived from HPV 16 were obteined by replacement of the FG loop of type 16 by type 31. HPV 31 pseudovirions were produced in mammalian cells to developp more sensitive neutralizing antibodies assay
Olivera-Botello, Gustavo. "Modélisation numérique des aspects immunologiques de la réaction à l'infection à HPV et de la vaccination anti-HPV par Gardasil®." Phd thesis, Université Claude Bernard - Lyon I, 2011. http://tel.archives-ouvertes.fr/tel-00846182.
Tang, Alexandre. "Rôle des lymphocytes B dans l'immunité anti-tumorale dans un modèle murin de cancer lié au papillomavirus humain (HPV)." Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCC248.
Enhancing ante-tumor immunity and preventing tumor escape are efficient strategies to increase the efficacy of therapeutic cancer vaccines. However, the treatment of advanced tumors remains difficult, mainly due to the immunosuppressive tumor microenvironment. Regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) have been extensively studied and their role in suppressing tumor immunity is now well established. In contrast, the role of B lymphocytes in tumor immunity remains unclear, since B cells can promote tumor immunity or display regulatory functions to control excessive inflammation, mainly through IL-10 secretion. Here, we demonstrate in a mouse model of HPV-related cancer that B cells play a detrimental role in anti-tumor immunity and participate in tumor promotion. Indeed, in B cell-deficient MuMT mice, the tumor growth was impaired and tumor rejection occurred due to a strong T cell dependent anti-tumor response. We show that B cells expressing PD-L1, CD39 and Ly6A/E markers accumulate in the tumor draining lymph node (dLN) which can directly impact T cell immunity. This inhibition is IL-10 independent since B cells from tumor-bearing mice did not show an increased ability to secrete IL-10 and deficiency in IL-10 production did not impair tumor growth. Furthermore, genetic analysis based on Single Nucleotide Polymorphisms (SNPs) also evidenced a relation between tumor rejection in MuMT mice and reactive oxygen species production and NK cell activity. Our results suggest that targeting B cell populations could enhance anti-tumor response and improve the efficacy of therapeutic cancer vaccines
Mandy, Muller. "La cartographie comparative des interactions E2-hôte révèle de nouveaux rôles de E2 dans la pathogénie associée aux papillomavirus humain." Phd thesis, Université Paris-Diderot - Paris VII, 2013. http://tel.archives-ouvertes.fr/tel-00881786.
Sandoval, Federico. "Optimisation d’un vaccin thérapeutique contre les tumeurs des voies aérodigestives supérieures associées au virus de papilloma humain (HPV) : Mise en évidence du rôle de la compartimentalisation de la réponse immunitaire antitumorale." Thesis, Paris 5, 2012. http://www.theses.fr/2012PA05T012.
Recent clinical trials have shown the therapeutic benefits of new promising immunotherapies (Sipoleucel T for prostate cancer, Ipilimumab in melanoma…). But by far, the majority of cancer vaccine clinical trials have shown modest clinical effects on cancer patients, contrasting with results found in preclinical models. Those preclinical models of cancer rely on subcutaneous grafts of tumor cells which do not mimic the true anatomic location of tumor lesions. In addition, in most cases cancer vaccines are administrated by systemic route, eliciting systemic antitumor responses and therapeutic effects. The antitumor response elicited by those vaccine strategies at the local environment of tumor location and their antitumor effect on orthotopic tumor models has not yet been addressed in preclinical cancer models. Since the majority of human tumors develop at mucosal surfaces, we addressed the question of the effect of the immunization route in the induction of local mucosal antitumor CD8+T cell responses by comparing a systemic intramuscular (i.m.) and intranasal (i.n.) route of administration of cancer vaccine. This vaccine consists of a non-replicative vaccine strategy that targets tumor antigen in vivo to dendritic cells developed at our laboratory and composed of the B subunit of the Shiga toxin (STxB) associated to a tumor antigen (E7 protein of HPV16). We also analyzed the antitumor effect of these vaccinations on two mucosal orthotopic tumor models of head and neck and lung cancer expressing the E7 antigen. We found that intranasal vaccination induced stronger specific CD8+T cell responses and antitumor effects at mucosal sites than systemic immunization, and, that mucosal vaccination induced a mucosal imprinting phenotype on mucosal derived antigen specific T cells as they expressed the mucosal integrins CD103 and CD49a, as opposed to systemic specific CD8+T cells or tumor infiltrating T cells in subcutaneous tumors. Inhibition of CD49a reduced the antitumor efficacy of the nasal vaccine and the number of tumor infiltrating CD8+T cells on orthotopic mucosal tumors. Our results showed that systemic antigen-specific T cell responses as typically assessed did not predict the quality of local mucosal immune response. Our observations provide direct evidence for the compartmentalization of mucosal tumor immunity, a critical finding for the rational design of better cancer vaccines
Desaintes, Christian. "Etude de la régulation transcriptionnelle par la protéine E6 du papillomavirus humain de type 16(HPV 16), et par la protéine cellulaire "suppresseur de tumeur" p53." Doctoral thesis, Universite Libre de Bruxelles, 1994. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212697.
Mourtada, Jana. "Mécanismes d’activation de la réponse immunitaire par DNp63 dans les cancers des voies aérodigestives supérieures HPV-positifs." Electronic Thesis or Diss., Strasbourg, 2023. http://www.theses.fr/2023STRAJ127.
HPV+ oropharyngeal tumors display both prognostic and molecular heterogeneity. Patients prognosis can be distinguished by the presence or absence of a molecular signature that depends on the ΔNp63 transcription factor. We demonstrated that ΔNp63 inhibits the migratory and invasive capabilities of HPV+ HNSCC cell lines and increases their sensitivity to platinum-based chemotherapy, implying its role in tumor progression. A functional analysis of ΔNp63 revealed its ability to stimulate the phagocytosis of cancer cells by macrophages in vitro. Consistently, a transcriptomic analysis of the same cellular model highlighted that ΔNp63 regulates the expression of secreted factors, including chemokines and interleukins, among which is the DKK3protein. Our findings indicate that DKK3 secretion by cancer cells activates the NF-κB pathway in macrophages, mimicking ΔNp63's effects on phagocytosis regulation. Induction of the NF-κB pathway by DKK3 in macrophages is mediated by its receptor CKAP4. Finally, our analyses suggest that ∆Np63 regulates the expression of factors involved in the inflammasome, as well as those of other cytokines such as TNFRSF11B, CCL26, CCL11, TIMP1 and TIMP2. Altogether, our results show that ΔNp63 plays a unique role in the prognosis of HPV+ patients by regulating secreted molecules involved in the recruitment and immune cell activation
Ben, Hadj Yahia Mohamed-Béchir. "Données et outils pour l'optimisation de l’impact de la vaccination prophylactique contre les papillomavirus humains en France." Thesis, Lille 2, 2015. http://www.theses.fr/2015LIL2S041/document.
Introduction: Since 2007, prophylactic vaccination against human papillomavirus (HPV) has been recommended in addition to cervical screening in French women. However, given the low vaccine coverage in France, the epidemiological impact of the vaccination is debated, as well as the choice of the target population and the means to ensure compliance with the recommendation. This doctoral thesis provides original data and tools for the evaluation and the improvement of the impact of HPV vaccination in France. For quantitative aspects, modelling HPV transmission based on the best data describing sexual partnerships in the general population is essential. The investigation of potential links between participation to cervical screening of deprived women and their choice of vaccinating their daughters, the appraisal of vaccine acceptability through social media and the cost-effectiveness evaluation of the relevance of extending the HPV vaccination program to include males are key elements to improve the focus on targeted populations.Methods: We developed a modelling platform to study the dynamics of HPV transmission, using data from Social Context of Sexuality, the latest national French sexual behavior study. Using finite mixture models, we identified latent classes of sexual activity to define profiles of partner acquisition with age, likely to have different risks of sexually transmitted infections. Then, we asked women attending the Centre for Preventive Medicine and Health Education of Lille, who had at least a daughter eligible for HPV vaccination, about their attitudes towards cervical screening and HPV vaccination. Next, we explored sentiments about HPV vaccine safety, efficacy and perceptions, spontaneously expressed by web users on the online discussion forum of a French-speaking health information website. Finally, we performed a systematic review of the cost-effectiveness studies about extending HPV vaccination to include males.Results: Simulations from the modelling platform reproduced HPV infection prevalence observed in France. Nevertheless, results were sensitive to assumptions about sexual behavior, with discrepancies between men and women. Five latent classes of sexual activity were identified in men and in women. The cluster describing the highest level of sexual behavior represents 3.3% in women and 4.8% in men. Besides, daughters’ vaccination profile did not differ with their mothers’ profile of participation to cervical screening. The main reason for not vaccinating their daughters reported by mothers was lack of information, especially for those non-compliant with cervical screening recommendations. Moreover, negative sentiments, reported by the health website forum, evolved from 28.6% of total opinions in 2006 to 42.2% in 2013. The arguments expressed by “anti-vaccine” postings involved most often vaccine safety and negative vaccine perceptions. Finally, cost-effectiveness analyses show that extending the HPV vaccination program to include males is rarely found to be a cost-effective strategy. Nevertheless, the targeted vaccination of men having sex with men seems to be the best strategy from ethical and cost-effectiveness points of view.Discussion: The modelling platform of sexual contacts represents the basis of the evaluation of HPV vaccination impact. The surveillance of online forums enables the monitoring of vaccine acceptability and hence the targeting of preventive messages. Improving the HPV vaccine coverage requires offering girls and young women an organized vaccination program. In the lack of a school-based vaccination program, Centres for Preventive Medicine and Health Education offer an interesting alternative
Nizard, Mevyn. "Optimisation d'un vaccin thérapeutique dans les tumeurs des voies aérodigestives supérieures associées aux papillomavirus : rôle de l'induction d'une immunité muqueuse et de la combinaison à la radiothérapie." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015PA05T027/document.
Cancer is the second mortality cause worldwide while mucosal cancers (lung, stomac, …) is the first mortality cause from. The majority of cancer vaccines against mucosal tumors have not given rise yet to significant clinical results. In this work we developed a strong immunotherapy based on the nontoxic subunit B from shiga toxin and showed for the first time that the localization of the immunization is crucial to induce potent and effective anti-tumoral responses. In a preclinical model a systemic immunization failed to induce a therapeutical protection against mucosal tumor challenge while intranasal immunization completely succeed. We identified a CD8 T lymphocyte population as a required cells in this protection and more precisely the T resident memory (Trm) cells. This Trm showed the classical CD103 phenotype as well as the CD49a which can play a specific role in the retention or the migration of this cells in the tumor tissue and might play a role in the survival. We also demonstrate that dendritic cells from the mucosal parenchyma was required to induce the CD49a expression on CD8 T cells while dendritic cells from the spleen was not. Our work shows that the Trm number as an impact in the anti-tumoral protection. We were able to reduce the Trm number in vivo using an anti-TGF-β antibody. This number diminution was correlated with a less efficient anti-tumoral protection. Patients with head and neck cancers are treated with radiotherapy. In this situation we showed that the combination of radiotherapy and our immunotherapy was associated with a better protection than radiotherapy alone or immunotherapy alone thanks to a vascular normalization. These results might rapidly lead to clinical trials and might open new ways to work with immunotherapies
Morel, Adrien. "Régulation épigénétique des gènes précoces d'HPV16." Thesis, Besançon, 2016. http://www.theses.fr/2016BESA3005.
High risk Human Papillomaviruses (HPV) are responsible for cervical cancer. HPV genome consists in a double-strand circular DNA harboring early "E" and late "L" genes and a Long Control Region (LCR). The E2 protcin binds to E2 Binding Sites (E2BS) present on the LCR and represses E6 and E7 transcription. The loss of E2 expression after HPV DNA integration induces an overexpression of E6 and E7 that thus favor p53 and pRb degradation. Since CpG dinucleotides are present in HPVl6 E2BS, we investigated whether E6 HPV16 expression was also submitted to epigenetic regulation. We developed a HRM PCR to study the methylation status of E2BS in precanccrous and canccrous lesions. We observed methylated CpG only in cancer samples. Otherwise, we proved that E2BS methylation prevented E2 binding and probably permitted E6 and E7 overexpression. Finally, we showed that the treatment ofHPV16 cervical cancer cell lines with a demethylating agent (SazadC) decreased the E6 expression. This regulation was independent of E2 and we proved that the up-regulation of miR-375, which targets E6/E7 transcripts, was involved in E6 repression after SazadC treatment. Taken as a whole, our data demonstrate that HPV 16 oncoprotein expression is regulated in an epigenetic manncr via viral and cellular factors
Brulet, Jean-Marc. "Cibler l’oncoprotéine E7 vers la voie de présentation du complexe majeur d’histocompatibilité de classe II: une piste vers un nouveau vaccin contre les lésions (pré)cancéreuses du col de l’utérus induites par le papillomavirus humain de type 16 (HPV-16)." Doctoral thesis, Universite Libre de Bruxelles, 2006. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/221575.
Doctorat en Sciences
info:eu-repo/semantics/nonPublished
Meys, Rhonda. "Aspects of human papillomavirus (HPV) disease in human immunodeficiency virus (HIV) infection." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/10730.
Raleigh, Sarah Elizabeth. "Hispanic Parents' Knowledge, Attitudes and Beliefs Toward Human Papillomavirus and Human Papillomavirus Vaccination in Arizona." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/612848.
Didelot, Céline. "Arrêts du cycle cellulaire et induction d'apoptose pour les lignées de carcinome humain de la tête et du cou HPV-18 positives, après exposition au 5-fluorouracile et aux radiations ionisantes : implication de NF-kB dans la radiosensibilité et l'apoptose spontanée." Nancy 1, 2002. http://docnum.univ-lorraine.fr/public/SCD_T_2002_0316_DIDELOT.pdf.
AZEVEDO, Karinne Silva. "Avaliação da prevalência do Papíloma Humano (HPV) em saliva de pacientes portadores do HIV." Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/18071.
Made available in DSpace on 2016-12-12T14:11:56Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) DISSERTACAO PARA BIBLIOTECA CENTRAL - KARINNE AZEVEDO.pdf: 2052240 bytes, checksum: fba514c37249c7db73fcf51a37fa21ff (MD5) Previous issue date: 2015-08-27
CAPES
Identificar a presença dos sorotipos de alto risco do Papilomavírus Humano (HPV) na saliva de pacientes portadores do vírus HIV. A amostra de 90 pacientes foi oriunda de dois centros de referência em tratamento de ISTs da cidade do Recife, PE, Brasil. Uma entrevista foi realizada para identificar o perfil da amostra, sendo realizada uma coleta de saliva empregando tubos falcon e solução para bochecho com sacarose a 5%, com posterior armazenamento em freezer a -20°C para rastreamento do HPV e genotipagem para o sorotipo 16 e 18 por PCR convencional. Na amostra predominou a presença do sexo masculino 59 de 90 (65,6%), com idade média de 38,8 anos, variando entre 18 e 69 anos, renda familiar média de 1,95 Salários Mínimos (DP = 1,37). A prevalência de HPV nesta amostra foi de 23 de 90 (25,6%) e dos sorotipos 16 e 18 foi 8 de 90 (8,9%). A co-infecção por HPV é comumente observada em pacientes portadores de HIV.
To identify the presence of high-risk serotypes human papillomavirus (HPV) in patients with sexually transmitted infections (STIs). A sample of 90 patients were from two referral hospitals in treatment of STIs. An interview was conducted to identify the sample’s profile a saliva collections being perfomed using falcon tubs and mount rinse with 5% sucrose, subsequente storage in a freezer at -20ºC for HPV screening and genotyping for serotype 16 and 18 by conventional PCR. In the sample predominant male presence 59 of 90 (65.6%) with mean age of 38.8 years, ranging between 18 and 69 years, average family income of 1.95 minimum wages (SD = 1, 37). The prevalence of HPV in this sample was 23 of 90 (25.6%) and the serotype HPV 16 and 18 was 8 of 90 (8.9%). Co-infection with HPV is commonly observed in HIV patients.
Jolly, Carol E. "The effects of leptomycin B on HPV-infected cells." Thesis, University of St Andrews, 2008. http://hdl.handle.net/10023/900.
Boulenouar, Selma. "Impacts of Human Papillomavirus type 16 (HPV-16) early proteins on trophoblastic cells." Doctoral thesis, Universite Libre de Bruxelles, 2010. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210188.
Six jours après la fécondation et suite à l’accolement du blastocyste à l’épithélium utérin, le trophoblaste s’engage dans des processus actifs de prolifération, d’invasion et de différenciation complexe pour la construction de l’interface physiologique indispensable aux échanges essentiels entre la mère et l’enfant ;le placenta. De façon intéressante, ses propriétés sont similaires à celles de la cellule tumorale maligne. Néanmoins, ses mécanismes sont étroitement régulés dans le trophoblaste, à la fois dans l’espace et le temps, assurant un développement normal à chaque étape de la grossesse.
Devant toutes ces données, nous avions émis l’hypothèse que l’expression des protéines précoces E5, E6 et E7 d’HPV de type 16 (de haut risque), pourraient modifier le développement des trophoblastes infectés. Les résultats obtenus durant ce travail de doctorat démontrent que la protéine virale E5, hautement hydrophobe, est cytotoxique et affecte la viabilité du trophoblaste. Cette cytotoxicité est neutralisée, et la viabilité est améliorée, lorsque les oncoprotéines majeures E6 et E7 sont exprimées en présence de la protéine E5. Lorsque toutes les protéines précoces sont exprimées sous le contrôle de leur propre promoteur (LCR), la viabilité est favorisée. Ces observations ont été confirmées dans les cellules cervicales également. Il a été précédemment rapporté que les oncoprotéines E6 et E7 affectaient l’adhésion du trophoblaste aux cellules endométriales. Dans le présent travail, il a été retrouvé que la protéine E5 diminuait elle aussi l’adhésion, non seulement aux cellules endométriales, mais aussi au support de culture cellulaire. Les capacités de migration et d’invasion de la matrice extracellulaire sont augmentées par l’expression de E5 et dans une plus large proportion par l’expression de E6 et E7. Des résultats similaires ont été obtenus lorsque toutes les protéines de la région précoces sont exprimées sous le contrôle de leur propre promoteur (LCR). La diminution de l’expression de la E-cadhérine est considérée comme un marqueur de malignité et de mauvais pronostic pour les cancers. Nous avons démontré que l’expression de E5, E6 ou de E7, inhibait l’expression de la E-cadhérine, reflétant l’impact des oncoprotéines du virus HPV-16 sur la diminution de l’adhésion et l’augmentation du pouvoir invasif des cellules trophoblastiques. L’investigation d’autres marqueurs de malignité et de tolérance immunitaire, l’étude de l’impact du virus HPV-6 (de bas risque) sur la migration et l’invasion des cellules trophoblastiques, et l’étude de la capacité des protéines précoces d’HPV-16 à influencer l’entrée des particules virales, ont fait l’objet de résultats préliminaires, ouvrant de larges perspectives.
Genital Human Papillomavirus (HPV) infections are the most common sexually transmitted infections amongst women on the age of reproduction. It is well established that persistent infection with high-risk HPVs is the necessary factor in the causation of precancerous and cancerous cervical lesions. High-risk HPVs have also been reported to be involved in the causation of head and neck cancers and other anogenital cancers. On this last decade, growing data are attempting to study the potential etiological association of HPV with gestational dysfunctions. The detection of HPV DNA in placentas resulting from spontaneous abortions and the unique ability of multiple HPV types to replicate in vitro in trophoblastic cells cultured in a monolayer system, rise new questions over the HPV tropism.
Six days following fertilization and once the apposition of the blastocyst on the uterine wall takes place, the trophoblast, in a very active and complex process, starts to proliferate, invade and to differentiate in order to build a physiological interface; the placenta, from where multiple mother/foetus exchanges occur. Interestingly, the way that the trophoblast behaves is very similar to malignant tumoural cells. However, the trophoblast obeys to strict spatial-temporal regulatory confines, insuring a proper development all along the pregnancy.
In regard to these data, we hypothesised that the expression of the high-risk HPV type 16 oncoproteins E5, E6 and E7, might modify the development of the infected trophoblast. During my Ph.D study, I demonstrated that the highly hydrophobic protein E5 is localized in many interne membranes compartments of the transfected trophoblast. E5 affects the viability of transiently and stably transfected trophoblastic cells. E6 and E7, favouring cell growth, neutralised the E5 cytotoxic effect. All HPV-16 early proteins, when expressed under the control of their endogenous promoter (LCR), favoured trophoblastic growth. These observations were also observed in cervical cell lines. In addition, E5 decreased the adhesiveness of trophoblastic cells to the tissue culture plastic and to endometrial cells similarly as previously described for E6 and E7. Cells expressing E6, E7 and in less extend E5 favoured chemotaxic migration and matrigel invasion compared to the cells expressing the LacZ control. These effects were also observed when early proteins were expressed under the control of their own viral promoter (LCR). Interestingly, the E-cadherin was down regulated in trophoblastic cells expressing E5, E6 and E7. In conclusion, HPV-16 early proteins enhanced trophoblastic growth and intensify the malignant phenotype by impairing cell adhesion leading to increased cellular motile and invasive properties. HPV-16 E5 participated, with E6 and E7, in these changes by impairing E-cadherin expression, a hallmark of malignant progression. Additional preliminary results consisting on the investigation of other markers of malignancy and immune tolerance, on studying the impact of the low-risk HPV type 6 early proteins on the migratory and invasive properties of trophoblastic cells and on the study of the ability of HPV-16 to influence the entry of virus particules, allowed to open wide perspectives.
Doctorat en Sciences
info:eu-repo/semantics/nonPublished
Dareng, Eileen Onyeche. "Human papillomavirus infections and human papillomavirus associated diseases in Nigeria : distribution, determinants and control." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/284551.
Nielson, Carrie. "Human Papillomavirus Prevalence in Asymptomatic Men." Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/194193.
Afrogheh, Amir. "The role of high-risk human papillomavirus in periocular cancers." University of the Western Cape, 2018. http://hdl.handle.net/11394/6554.
PURPOSE: High risk human papillomavirus (HR-HPV) is well established as a causative agent of squamous cell carcinoma (SCC) of the orophaynx. HR-HPV has also been reported in periocular cancers and precancers, but controversy exists about its overall incidence and clinicopathologic profile. The purpose of this study is to evaluate the role of HR-HPV infection in periocular cancers and precancers, using multiple methods of detection. DESIGN: Retrospective observational case series with laboratory investigations. METHODS: Sequential surgical samples of 87 carcinomas (invasive SCC, SCC in situ and sebaceous carcinoma) from three different periocular sites (conjunctiva, lacrimal sac and the eyelid) diagnosed over a 15-year period (2000-2015) were selected for evaluation. Unstained paraffin sections of 87 cases of periocular carcinomas were analyzed with immunohistochemistry (IHC) for p16 as a screening test. p16 positive conjunctival- and lacrimal sac SCC were further evaluated for HR-HPV using DNA in situ hybridization (DNA ISH), and a subset was also analyzed by DNA Polymerase Chain Reaction (DNA PCR). p16 positive periocular sebaceous carcinomas (SC) were analyzed with PCR, and a subset of 18cases was further studied with a novel method of mRNA ISH, an advanced technique with an enhanced sensitivity and specificity. Relevant patient clinical information was obtained from review of the electronic medical records.
Leung, Tiem-yee, and 梁湉兒. "Literature review on parental acceptability of human papillomavirus (HPV) vaccine." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46939003.
MATTOS, A. T. "Genotipagem Molecular de HPV Proveniente de Mulheres Soropositivas e Soronegativas para HIV Atendidas no Centro de Referência em DST/AIDS." Universidade Federal do Espírito Santo, 2010. http://repositorio.ufes.br/handle/10/4530.
Os HPV são vírus epiteliotrópicos que infectam tecido cutâneo ou mucoso e estão relacionados com desenvolvimento de lesões que, no trato genital, variam de verrugas ao câncer cervical invasivo. As lesões são causadas por diferentes tipos de HPV, que são classificados em baixo e alto risco conforme sua associação com câncer cervical. Sabe-se que mulheres positivas para HIV são mais acometidas por infecções por HPV e estão mais propensas ao desenvolvimento de câncer cervical. O objetivo desse estudo foi avaliar a frequência de tipos de HPV em mulheres soropositivas e soronegativas para HIV. Para isso foram analisadas amostras de escovado cervical, mantidas congeladas, de mulheres conhecidamente positivas para HPV (n=87), atendidas no Centro de Referência DST/AIDS, em Vitória-ES, no período de março a dezembro de 2006. O DNA das amostras foi extraído utilizando kit comercial QIAamp® DNA Mini Kit ou através do método de isotiocinanato de guanidina e sílica. DNA do HPV foi amplificado por PCR utilizando os iniciadores degenerados MY09/MY11 e a genotipagem foi realizada por Restriction Fragment Length Polymorphism (RFLP) e por Reverse Line Blot (RLB). Do total de amostras, 97,7% foram genotipadas e 31 tipos distintos detectados: 6, 11, 13, 16, 18, 26, 31, 31b, 32, 33, 34, 35, 42, 44, 51, 52, 53, 55, 56, 58, 59, 61, 62, 64, 66, 68, 71, 81, 82, 83 e 84. O tipo mais prevalente foi o HPV16, tanto nas mulheres soropositivas quanto nas soronegativas para HIV, seguido pelos tipos 6, 53 e 11. O tipo 13, incomum em amostras cervicais, foi observado nesse estudo, porém a quantidade de amostras não foi suficiente para a realização de seqüenciamento para a confirmação deste tipo viral. Os tipos oncogênicos foram mais comuns nas amostras de mulheres soropositivas para HIV, porém com número semelhante e o número de infecções múltiplas foi maior entre as mulheres HIV positivas. Este estudo revelou uma grande diversidade de tipos de HPV na região. PALAVRAS CHAVES: Human papillomavirus (HPV), Human Immunodeficiency virus (HIV), Restriction Fragment Length Polymorphism (RFLP), Reverse Line Blot (RLB).
Ebertz, Barika. "Factors influencing women's intentions to obtain the Human Papillomavirus (HPV) vaccine." Thesis, Högskolan Kristianstad, Sektionen för hälsa och samhälle, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-10745.
Bakgrund: Cervixcancer är den näst vanligaste cancern hos kvinnor med en global incidens på15 %. Cervixcancer leder till hög mortalitet. Genom Humant Papillomvirus (HPV)-vaccinering kan incidensen minskas kraftigt. Vaccintäckningen är suboptimal på många plaster i världen. Det är viktigt att vårdpersonal, inklusive sjuksköterskor, förstår vilka faktorer som påverkar viljan och beslutet att vaccinera sig. På så sätt kan sjukvårdspersonal påverka dessa beslut och faktorer och därigenom öka vaccinationstäckningen i befolkningen. Syfte: Syftet var att beskriva faktorer som påverkar kvinnors avsikt till att vaccinera sig mot HPV. Metod: I denna allmänna litteraturstudie användes databaserna Cinahl, Medline, PsycINFO, Summon @ HKR and Pubmed för att söka efter artiklar som studerade faktorer som påverkar kvinnor att vaccinera sig mot HPV. Totalt tio artiklar inkluderades, fem kvalitativa och fem kvantitativa studier. Resultat: Fyra huvudkategorier identifierades som påverkade kvinnor att vaccinera sig mot HPV: Kunskap, attityder, andras inflytande och vaccinets säkerhet. Diskussion: Bättre tillgång till korrekt information för kvinnor om HPV-vaccinet är nyckeln till att öka kvinnors avsikt att vaccinera sig och på så sätt förbättra folkhälsan. Slutsats: Det krävs korrekt information om HPV virus och vaccin för att öka kvinnors avsikt till att vaccinera sig.
Jalil, Emilia Moreira. "Prevalência da infecção pelo papilomavírus humano (HPV) em gestantes infectadas ou não pelo vírus da imunodeficiência humana (HIV) tipo 1 em Ribeirão Preto, SP." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-06032008-102658/.
Genital infection with human papillomavirus (HPV) is considered to be the most frequent sexually transmitted disease around the world, representing an important public health problem due to its high prevalence and transmissibility. It is estimated that 75% of the sexually active population gets in contact with one or more HPV types during their lives, with higher prevalence among younger women. Epidemiologic studies have demonstrated that human immunodeficiency virus (HIV) infection is associated with a high prevalence of HPV infection. The literature about HPV infection during pregnancy is scarce and controversial. The aim of this study was to estimate the prevalence of HPV infection in pregnant women and identify the possible influence of HIV-1 infection on this prevalence. Patients were selected from the Prenatal Outpatient Clinic of the Infectious Diseases Sector and from the Low-risk Prenatal Outpatient Clinic of the Obstetrics and Gynecology Department of the University Hospital, Medical School of Ribeirão Preto, São Paulo University. All patients were informed about the study and signed an informed consent term. Cervical-vaginal washes were collected and submitted to DNA extraction by the salting-out technique. HPV was detected in the DNA samples by the polymerase chain reaction (PCR) technique and the HPV-positive samples were tested for types 6, 11, 16 and 18. Ninety-seven patients were included in the study, 44 of them being HIV-positive and 53 HIV-negative. Of the patients evaluated, 66 were positive for HPV. The prevalence of HPV infection was 79.5% and 58.5% in HIV-positive and -negative women, respectively. HIV infection increased the risk of harboring HPV, mainly oncogenic types. A CD4+ T-cell count below 200 cells/mm3 and HIV viral load above 10000 copies/mL increased the risk of HPV infection. This study showed a higher prevalence of HPV infection in HIV-positive pregnant women, suggesting that this retrovirus infection is a significant risk factor for the increase of HPV infection in pregnant women.
Rodrigues, Michelle Christine Carlos. "DETECÇÃO E GENOTIPAGEM DE MÚLTIPLOS TIPOS DO PAPILOMAVÍRUS HUMANO (HPV) EM MULHERES HIV-POSITIVAS E HIV-NEGATIVAS EM GOIÂNIA-GO." Pontifícia Universidade Católica de Goiás, 2011. http://localhost:8080/tede/handle/tede/2341.
HIV-infected women are more likely to be infected with high-risk HPV genotypes that have the potential for progressing to cervical cancer. To Know the prevalence of type-specific human papillomavirus (HPV) infections in HIV-infected women is necessary in order to plan effective screening and preventive strategies in such population. Here, we compare the prevalence of infections with multiple HPV types and cytological abnormalities in two groups of women, HIV-positive and HIVnegative, in the city of Goiânia-GO, Brazil. Cervical smears obtained from 57 HIVpositive and 57 HIV-negative women were collected in a preservative medium and submitted to DNA extraction. HPV-DNA detection and genotyping were performed by using the Linear Array HPV Genotyping Test according to the manual provided by the manufacturer. Both groups were similar in regard to social aspects, demographics and behavioral characteristics. HPV DNA was significantly more prevalent in HIV-positive women, compared to HIV-negative women (56.7% vs. 28.3%, p = 0.003). Coinfections with HPV multiple genotypes was also more prevalent in the HIV-positive group (88.2% vs. 58.8%, p = 0.028). HPV16 was the most prevalent in both groups. Cytological abnormalities were observed in 5.3% of HIV-negative women and in 29.8% HIV-positive women. The presence of HPV was significantly associated with cytological abnormalities only in HIV-negative women. Our results demonstrated a greater prevalence of HPV infection in the HIV-positive group of women, with a greater prevalence of infection with multiple genotypes. We here emphasize the need of frequent monitoring of HIV-infected women, in order to allow early detection and efficient treatment for cervical intraepithelial neoplasia (CIN) in this high-risk group.
Mulheres infectadas pelo HIV são mais susceptíveis à infecção por HPVs de alto risco oncogênico, que apresentam um potencial para a progressão para o câncer cervical. Conhecer a prevalência dos tipos específicos do papilomavirus humano nas mulheres HIV infectadas é necessário para planejar um rastreamento eficaz e estabelecer estratégias preventivas em tal população. Aqui, pudemos comparar a prevalência de infecções por múltiplos genótipos de HPV e de alterações citológicas em dois grupos de mulheres, HIV-positivas e HIV-negativas, da cidade de Goiânia- GO, Brasil. Esfregaços cervicais obtidos de 57 mulheres HIV-positivas e 57 mulheres HIV-negativas foram colhidos em meio conservante e enviadas para extração de DNA. A detecção de DNA e genotipagem do HPV foram realizadas usando o Kit Linear Array HPV Genotyping Test, de acordo com o manual fornecido pelo fabricante. Ambos os grupos foram semelhantes no que diz respeito à demografia, aos aspectos sociais e comportamentais. A detecção do DNA de HPV foi significativamente mais prevalente em mulheres HIV positivas, em comparação com as mulheres HIV-negativas (56,7% vs. 28,3%, p = 0,003). Coinfecção por vários genótipos de HPV também foi mais prevalente no grupo de HIV-positivas (88,2% vs. 58,8%, p = 0,028). O HPV16 foi o genótipo mais prevalente em ambos. Anormalidades citológicas foram observadas em 5,3% das mulheres HIV-negativas e 29,8% das mulheres HIV-positivas. A presença de HPV foi significativamente associada com anormalidades citológicas somente nas mulheres HIV-negativas Nossos resultados demonstraram uma maior prevalência de infecção por HPV no grupo de mulheres HIV-positivas, com um elevado número de casos de infecção por vários genótipos. Enfatizamos aqui a necessidade de monitoramento frequente de mulheres infectadas pelo HIV, de modo a permitir o diagnóstico precoce e tratamento eficaz para neoplasia intra-epitelial cervical (NIC) neste grupo de alto risco.
Defayette, D. Nicole, and L. Lee Glenn. "Marginal Effects of Patient Age on Human Papillomavirus Knowledge." Digital Commons @ East Tennessee State University, 2012. https://dc.etsu.edu/etsu-works/7486.
Ariyo, Oluwatosin. "Correlates of Human Papillomavirus (HPV) Vaccine Acceptance in Appalachian Tennessee." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etd/3238.
Majeed, Gulnaz Syed. "Cytokine polymorphisms in low grade HPV associated cervical lesions." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368642.
Johnson, Chandrika. "College Students' HPV Knowledge and Intention to be HPV Vaccinated." OpenSIUC, 2014. https://opensiuc.lib.siu.edu/dissertations/954.
Weyn, Christine. "Human papillomavirus prevalence and expression in trophoblastic and cervical cells." Doctoral thesis, Universite Libre de Bruxelles, 2010. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210012.
Vertical transmission of HPV was also previously reported, but in most cases one could not exclude a placental contamination by HPV positive cells from an infected birth canal. In order to confirm that the placenta can be infected with HPV, we analysed residual cells from 35 transabdominally obtained placental samples from pregnant women undergoing chorionic villous sampling for screening of suspected foetal abnormalities and found that two samples were positive for HPV-16 and HPV-62, respectively. The clinical importance of these results remains to be elucidated, but the previously observed association between placental HPV infection and pregnancy loss might gain further in importance. HPV gene regulation in placental trophoblastic cells has not been studied so far. We studied the HPV-16 early gene expression regulation in transiently transfected monolayer cultured trophoblastic cells with an HPV-16 long control region (LCR) driven reporter plasmid. We observed important differences in constitutive HPV-16 LCR activities between trophoblastic cell lines and could identify progesterone as an important inducer of HPV-16 early gene expression. Steroid hormones are induced during pregnancy and could therefore lead to an enhanced expression of the E5, E6 and E7 proteins upon placental HPV infection. Since these proteins were previously shown to affect trophoblast adhesion, survival, migration and invasion, their enhanced expression might eventually contribute to pregnancy loss. We furthermore found that the transcription of episomally maintained HPV-16 is not regulated by E2 or E1, but by E5, E6 and/or E7.
Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished
Weller, Giselle Schneider. "HPV-Related Stigma." University of Cincinnati / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1178880918.
Medeiros, Edinilza da Silva Machado. "CONHECIMENTO DE ENFERMEIRAS ACERCA DA VACINA ANTI-HPV, INFECÇÃO PELO HPV E CÂNCER DO COLO UTERINO." Pontifícia Universidade Católica de Goiás, 2016. http://tede2.pucgoias.edu.br:8080/handle/tede/3537.
Made available in DSpace on 2016-11-10T11:31:22Z (GMT). No. of bitstreams: 1 EDINILZA DA SILVA MACHADO MEDEIROS.pdf: 5001760 bytes, checksum: b5b35c8438c5ad0ee37d875a2d0aef41 (MD5) Previous issue date: 2016-03-14
This is a cross-sectional cohort study, descriptive with quantitative approach, performed in Public Health Units of cities inside of Bahia, Brazil, with 33 professionals. Data were collected through a multiple-choice questionnaire that intended to investigate the knowledge of the anti-HPV vaccine, HPV infection and cervical cancer, as well as seek association between knowledge with time and institution of training, participation in continuing education and hours worked per week. Data were analyzed using the SPSS® statistical package, version 23. It was adopted a level of significance of 5% (p <0.05). For the verification of possible associations, it was adopted the Pearson's chi-square (χ2) using the verisimilitude ratio coefficients. The results showed nurses with an average age of 30.6 (± 7.3) years; more than 80% from private institution; 51.5% with training time between 2 and 5 years; 84.8% with participation at permanent education; 39.4% with a work load of over 40 hours. All of them knew of the HPV virus and the sexual transmission. However, few knew the other forms of transmission, the effectiveness of condoms, classification and purpose of the Pap test. 90.9% of Nurses understood the role of HPV in the genesis of cervical cancer and genital warts. 97.0% knew the anti-HPV vaccine, but 51.5% thought that only women could be immunized. There was no significant association between time and institution of training, participation in permanent education, as well as hours of weekly work with the knowledge of these professionals on the subject. The study revealed that the nurses have divergent knowledge about the anti-HPV vaccine, HPV infection and cervical cancer, which justifies the needing for improvement of these professionals.
Trata-se de um estudo de corte transversal, de caráter descritivo com abordagem quantitativa, realizado em Unidades Públicas de Saúde de um Município do interior da Bahia, Brasil, com 33 profissionais. Os dados foram coletados por meio de um questionário contendo questões de múltipla escolha que buscaram investigar o conhecimento sobre a vacina anti-HPV, a infecção pelo HPV e o câncer de colo uterino, além de buscar associação entre o conhecimento com o tempo e instituição de formação, participação em educação permanente e carga horária de trabalho semanal. Os dados foram analisados por meio do pacote estatístico SPSS®, versão 23. Adotou-se um nível de significância de 5% (p < 0,05). Em verificação das possíveis associações, adotou-se o teste do Qui-quadrado de Pearson (χ2), utilizando os coeficientes da razão de verossimilhança. Os resultados mostraram Enfermeiras com média de idade de 30,6 (± 7,3) anos; mais de 80% proveniente de instituição particular; 51,5% com tempo de formação entre 2 e 5 anos; 84,8% com participação em educação permanente; 39,4% com carga horária de trabalho de mais de 40 horas. Todas sabiam da existência do vírus HPV e a transmissão pela via sexual. Todavia, poucas conheciam as demais formas de transmissão, a eficácia do preservativo, a classificação e objetivo do exame de Papanicolau. Entendiam o papel do HPV na gênese do câncer cervical e das verrugas genitais 90,9% das Enfermeiras. 97,0% conheciam a vacina anti-HPV, mas 51,5% achavam que apenas as mulheres poderiam ser imunizadas. Não houve associação significativa entre tempo e instituição de formação, participação em educação permanente, bem como carga horária de trabalho semanal com o conhecimento destas profissionais sobre a temática. Ficou evidenciado que as Enfermeiras possuem conhecimentos divergentes sobre a vacina anti-HPV, infecção pelo HPV e câncer de colo uterino, o que justifica a necessidade de aprimoramento por partes destas profissionais.
Southern, Shirley Anne. "Karyotypic analysis of cervical neoplasia : chromosomal aberrations and human papillomavirus infection." Thesis, University of Liverpool, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367063.
Winer, Rachel L. "Genital HPV infection and E7 mRNA viral load : incidence, risk factors, and relations to genital neoplasias /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/10917.
D'Ottaviano, Maria Gabriela Loffredo 1969. "Detecção dos tipos de HPV e integração do HPV DNA 16 em mulheres com NIC 2 seguidas por doze meses = HPV detection and HPV DNA 16 integration in women with CIN2 followed up for 12 month." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310566.
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2018-08-21T17:52:24Z (GMT). No. of bitstreams: 1 D'Ottaviano_MariaGabrielaLoffredo_D.pdf: 1516066 bytes, checksum: 6d0ec9bd4a1234053c224b579db92914 (MD5) Previous issue date: 2012
Resumo: A infecção pelo HPV é considerada fator etiológico da neoplasia do colo do útero e a integração do HPV DNA ao DNA da célula hospedeira são apontados como passo importante na carcinogênese do epitélio. O melhor conhecimento da infecção do vário tipo de HPV e o status físico do HPV 16 nas NIC 2 pode colaborar na identificação das lesões que teriam maior risco de progredir para NIC 3 e, portanto, deveriam ser consideradas como lesões precursoras do câncer do colo uterino. O objetivo desta série de casos foi descrever a presença dos diferentes tipos de HPV e a integração do HPV DNA 16 em mulheres com diagnóstico histológico de NIC 2 acompanhadas por 12 meses. Trinta e sete mulheres com citologia inicial, resultado de lesão de baixo grau e atípicas de células escamosas de significado indeterminado e NIC 2, confirmado por biópsia, foram seguidas por 12 meses com citologia, colposcopia, tipagem de HPV e determinação do status físico do HPV DNA 16 a cada três meses. A evolução clínica da NIC 2 foi classificada como regressão em 49% (18\37) dos casos, persistência em 22% (8\37) e progressão em 29% (11\37). A infecção por múltiplos tipos de HPV foi observada em 41% (15\37) dos casos na admissão e durante o seguimento 54% (20\37) dos casos apresentaram infecção por novos tipos de HPV. O HPV 16 foi considerado como possível causa em 67% (10\15) dos casos que persistiram ou progrediram e em 10% (1\10) dos que regrediram (p=0,01). Entre as 20 mulheres que apresentaram HPV 16 na admissão, a forma integrada foi detectada em 25% dos casos e a forma episomal em 75% dos casos. Não foram observados casos de progressão para NIC 3 sem integração do HPV DNA 16 em algum momento do seguimento. Entretanto, foram observados casos de integração do HPV DNA 16 e regressão da NIC 2. Concluindo, a infecção por múltiplos tipos de HPV é frequente nas mulheres com diagnóstico histológico de NIC 2, assim como a infecção por outros tipos de HPV durante o seguimento de 12 meses. As NIC 2 associadas à detecção do HPV 16 persistem ou progridem com maior frequência. As NIC 2 que progrediram para NIC 3 apresentaram o HPV DNA 16 na forma integrada na admissão ou em algum momento do seguimento
Abstract: Human papillomavirus (HPV) persistent infection is considered a necessary cause for the development of cervical cancer and HPV DNA integration considered an important step in the progression of persistent high risk HPV infection to invasive cancer.The knowledge of HPV infection and the HPV DNA 16 physical status in women with cervical intraepithelial neoplasia grade 2 (CIN 2) can better characterize the biological behavior of the lesion. This case series aimed to describe the HPV types and HPV DNA 16 physical status in women with CIN 2 biopsy proven followed for 12 months and clinical outcome. Thirty seven women with CIN 2 biopsy proven, cervical referral smear showing low-grade squamous intraepithelial lesions or atypical squamous cells of undetermined significance and with HPV type, were followed up 12 months with cervical smear, colposcopy, HPV type and HVP DNA 16 every three months. At the end of twelve months follow-up, the CIN 2 regression rate was 49% (18/37), persistence as CIN1 or CIN 2 was 22% (8/37), and progression to CIN 3 was 29% (11/37). Multiple HPV types were observed at admission in 41% (15/37) of cases. During follow-up, 54% (20/37) of the women showed one or more new HPV type detected. HPV 16 was considered possibly causal type in 67% (10/15) of the cases that persisted or progressed and in 10% (1/10) that regressed (p=0.01). Among the twenty women with HPV DNA 16, at admission, 25% showed integrated HPV DNA 16 and 75% episomal form. There were no cases of CIN 2 progression to CIN 3 without HPV DNA 16 integration, but there were cases of HPV DNA 16 integration and CIN 2 regression. Concluding, multiple HPV infections were frequently detected among women with CIN 2 at admission and during the follow up. The CIN 2 associated with HPV 16 was more likely to persist or to progress to CIN 3. The HPV DNA 16 integration is associated with CIN 2 persistence and progression to CIN 3
Doutorado
Oncologia Ginecológica e Mamária
Doutora em Ciências da Saúde
Subramaniam, Natasha Marie. "Addressing Human Papillomavirus Vaccination in Primary Care Pediatrics." ScholarWorks, 2019. https://scholarworks.waldenu.edu/dissertations/7434.
Stewart, Deborah. "P53 regulatory mechanisms by human papillomavirus (HPV) E6 and alternative splicing." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=85651.
In this study, we characterized two important regulatory mechanims of p53 activity: (i) Human papillomavirus (HPV) E6 interaction and (ii) alternative splicing. Recognized as the major etiological agents for cervical cancer, the oncogenic potential of HPVs correlates with their ability to target p53 for degradation. This study demonstrates that both p53 and HPV-18 E6 are exported from the nucleus when co-expressed, via a process that involves the C-terminal nuclear export signal (NES) of p53. However, neither nuclear export nor the p53 C-terminal NES is required for HPV-18 E6-mediated ubiquitination or degradation of p53.
This study also demonstrates that both low- and high-risk HPV E6 proteins are degraded by the ubiquitin-proteasome pathway, and thus provides an explanation for the low levels of E6 detected in cervical cancer cells.
Also reported in this study is a novel mechanism of p53 regulation arising through alternative splicing. This novel mRNA encodes a N-terminal deleted isoform of p53, termed p47. As demonstrated within, p47 does not supress cell viability but impairs both p53-mediated transcriptional activity and growth suppression. Interestingly, p47 increases both p53 monoubiquitination and nuclear export. We propose that p47 induces nuclear export of p53 by a mechanism involving monoubiquitination, as supported by recent findings from Li and colleagues (2003). The p47 protein also protects p53 from both Mdm2- and HPV-18 E6-mediated degradation. A number of cancers display abnormal localization of wildtype p53, and it will be important to examine the role of p47 in these tumours.
Taken together, the regulation of p53 activity by both HPV E6 and the alternative splice variant p47 involves alterations in p53 ubiquitination status, protein stability, and cell localization. Insight gained into these negative regulatory mechanisms may aid in the design of therapeutic strategies for reactivating wild-type p53 in HPV-associated and non-associated cancers.
Batista-Ferrer, Harriet. "Factors influencing the uptake of the Human Papillomavirus (HPV) vaccination programme." Thesis, University of Bristol, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.682349.
Dugoni, Meredith L. "Role of the Pediatric Dental Provider in Human Papillomavirus (HPV) Education." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4733.
RONGA, LUIGI. "Human papillomavirus (HPV): space-time epidemiology and issues concerning laboratory diagnosis." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2010. http://hdl.handle.net/2108/208544.
Muusha, Prudence. "Prevalence of Human papillomavirus among women following HPV vaccine introduction; a systematic review." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29833.
Eisenberg, Dana J. "Information Amount and Patient Empowerment: Participation in the HPV Vaccination Decision-Making Process." Columbus, Ohio : Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1243830226.
Stridh, Sandra, and Solvind Hammar. "Knowledge of Human papillomavirus (HPV) and attitudes towards HPV-vaccine among Thai female university students." Thesis, Uppsala universitet, Institutionen för folkhälso- och vårdvetenskap, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-214748.
Brotherton, Julia Mary Louise. "Human papillomavirus vaccination in Australia: assessing coverage and developing surveillance strategies." Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/12112.
Carrillo-Ng, Hugo, Lorena Becerra-Goicochea, Yordi Tarazona-Castro, Luis Pinillos-Vilca, Valle Luis J. Del, Miguel Angel Aguilar-Luis, Carmen Tinco-Valdez, et al. "Variations in cervico-vaginal microbiota among HPV-positive and HPV-negative asymptomatic women in Peru." BioMed Central Ltd, 2021. http://hdl.handle.net/10757/655810.
Revisión por pares
Ryan, Chelsea N., Kathryn L. Duvall, Emily C. Weyant, Kiana R. Johnson, and David L. Wood. "Human Papillomavirus Vaccine Uptake, Knowledge, and Acceptance for Youth: A Systematic Review of Appalachia." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/asrf/2018/schedule/15.