Дисертації з теми "Paper-based diagnostics"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-21 дисертацій для дослідження на тему "Paper-based diagnostics".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Miller, Eric Alexander. "Development of thermostable affinity reagents for low-cost, paper-based medical diagnostics." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122849.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references.
The timely diagnosis and treatment of disease in resource-constrained settings requires the development of robust point-of-care (POC) diagnostics, which provide accurate results and can be employed by users with minimal medical training and limited access to basic infrastructure. One of the most common POC diagnostic formats is the immunochromatographic rapid diagnostic test, which traditionally uses nitrocellulose-immobilized IgG antibodies as binding proteins for the capture of disease biomarkers from patient samples. However, these antibodies are expensive to produce and structurally complex, and are prone to thermal denaturation. In contexts where continuous cold chain storage may be infeasible, the resulting loss in binding activity can manifest as diminished assay sensitivity, leading to adverse clinical outcomes and eroding patient trust in the diagnostic format.
In the interest of replacing diagnostic antibodies with a more cost-effective, robust class of binding proteins, this thesis explores the development of thermostable affinity reagents based on the hyperthermophilic scaffold protein rcSso7d. Given its native microbial host, minimalist structure, and high wild-type melting temperature (98°C), rcSso7d represents a viable alternative to antibodies in in vitro POC assays. To assess the applicability of the rcSso7d scaffold in this context, protein engineering techniques were used to rapidly select analyte-specific binding variants from a yeast surface display library of >109 members. A high-affinity rcSso7d binder was identified, produced in high yield in a bacterial host, and readily purified in a single chromatographic step.
The in vitro activity and thermal stability of this engineered binder were characterized in the context of a low-cost, paper-based assay, and significant improvements in stability and production economics were observed for rcSso7d-based assays, relative to assays featuring a representative antibody reagent. Additionally, general strategies were developed to improve the diagnostic performance of paper-based assays employing rcSso7d-based reagents. In one instance, chimeric protein constructs in which rcSso7d variants are fused to a cellulose-binding domain were found to bind to unmodified cellulose in an oriented fashion and with high efficiency. This substrate anchoring approach permits the rapid, high-density immobilization of the rcSso7d species in paper-based assays, and yields significant sensitivity enhancement by enabling both the depletion of the soluble analyte from the sample, and the processing of large sample volumes within clinically relevant timescales.
Detection reagents incorporating rcSso7d binders were also developed, using novel fusion constructs which enabled in vivo labelling while preserving analyte binding activity. These techniques were applied in the context of a urine-based tuberculosis biomarker, and may one day permit the development of multiplexed assays targeting a suite of these analytes. Such a development would enable point-of-care diagnostic testing without requiring the production of sputa, facilitating disease detection in otherwise inaccessible patient populations (e.g. children under five, the elderly, and immunocompromised patients).
"People who have financially supported this thesis: the NIH Biotechnology Training Program, the Tata Center for Technology and Design, the Deshpande Center, the Sandbox program, and the Singapore- MIT Alliance for Research and Technology"
by Eric Alexander Miller.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Chemical Engineering
Ward, Jennifer Guerin. "Nitrocellulose Paper Based Microfluidic Platform Development and Surface Functionalization with Anti-IgE Aptamers." DigitalCommons@CalPoly, 2012. https://digitalcommons.calpoly.edu/theses/746.
Повний текст джерелаLama, Lara. "Novel methods for improving rapid paper-based protein assays with gold nanoparticle detection." Licentiate thesis, KTH, Proteomik och nanobioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-214065.
Повний текст джерелаQC 20170911
Glavan, Ana. "Chemical Approaches to the Surface Engineering of Paper and Cellulose-Based Materials for Microfluidics, Electronics and Low-Cost Diagnostics." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:26718749.
Повний текст джерелаChemistry and Chemical Biology
Katis, Ioannis. "Laser direct write techniques for the fabrication of paper-based diagnostic devices." Thesis, University of Southampton, 2015. https://eprints.soton.ac.uk/388397/.
Повний текст джерелаTageson, Mackenzie Elizabeth. "FUNCTIONAL 3-D CELLULOSE & NITROCELLULOSE PAPER-BASED, MULITPLEX DIAGNOSTIC PLATFORMS WITHOUT COUPLING AGENTS." DigitalCommons@CalPoly, 2013. https://digitalcommons.calpoly.edu/theses/1128.
Повний текст джерелаBen, Aissa Soler Alejandra. "Rapid diagnostic test for the detection of communicable diseases." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2020. http://hdl.handle.net/10803/670392.
Повний текст джерелаLa prevención y el control de las enfermedades transmisibles dependen, en gran medida, de la detección rápida y eficaz. Los métodos convencionales para la detección de un patógeno, como el cultivo microbiológico, generalmente requieren mucho tiempo, son laboriosos, necesitan personal cualificado y no son aptos como herramientas de diagnóstico en el punto de atención. El desarrollo de métodos de diagnóstico rápido en el marco de los criterios ASSURED, del inglés (A) Affordable, (SS) Sensitive and Specific, (U) User-friendly, (R) Rapid and Robust, (E) Equipment free, and (D) Deliverable to those who need it, Affordable, descritos por la Organización Mundial de la Salud (OMS), se encuentran en la actualidad bajo intenso estudio. Por lo tanto, la presente tesis aborda el diseño y desarrollo de estrategias, métodos y materiales para mejorar las prestaciones analíticas y simplificar el procedimiento en pruebas de diagnóstico rápido, incluidas nuevas estrategias de preconcentración en fase sólida, métodos de amplificación y materiales avanzados, así como su integración en diferentes plataformas (principalmente biosensores basados en detección electroquímica y pruebas en papel con lectura óptica). En todos los casos, las aplicaciones seleccionadas se centran en enfermedades transmisibles, incluidos los patógenos transmitidas por los alimentos y las micobacterias. Con este fin, se comparan dos plataformas basadas en papel en diferentes configuraciones (flujo lateral y vertical) en términos del rendimiento analítico para la detección de Mycobacterium. Para lograr una mejora adicional en el límite de detección, se estudia la preconcentración previa de las bacterias por separación inmunomagnética. En segundo lugar, se evalúan y se comparan en términos de su rendimiento analítico la detección simultánea de Salmonella y E. coli mediante flujo lateral de ácido nucleico con lectura visual y genosensores electroquímicos. Si bien estos métodos requieren PCR de doble etiquetado para la amplificación, se pueden adaptar fácilmente a termocicladores portátiles que funcionan con baterías para poder ser realizados en entornos con recursos limitados para satisfacer las demandas de diagnóstico ASSURED. Además, también se presenta en esta disertación la síntesis de polímeros magnéticos impresos molecularmente, con el objeto de reemplazar las partículas magnéticas biológicamente modificadas, y tomando como modelo la detección de biotina y moléculas biotiniladas. Además, se realiza la caracterización del material mediante diferentes técnicas analíticas y se compara, en todos los casos, con el polímero no impreso. Este material biomimético muestra un gran potencial para la preconcentración y detección de una amplia gama de analitos. A pesar de todo este progreso, las técnicas de amplificación de ácido nucleico siguen siendo necesarias para alcanzar los límites de detección requeridos en algunas enfermedades transmisibles. Las técnicas de amplificación isotérmica son buenos candidatos para llevar pruebas de diagnóstico en entornos donde la PCR puede ser una barrera. En concreto, se describe en esta disertación la detección de E. coli mediante un genosensor electroquímico basada en la amplificación isotérmica. En este caso, se optimiza la lectura electroquímica por voltamperometría de onda cuadrada en electrodos desechables comparando dos estrategias de marcaje del producto amplificado. Es importante resaltar que todas estas estrategias apuntan a ser utilizadas como herramientas para mejorar las pruebas de diagnóstico rápido en entornos de bajos recursos, para interrumpir la cadena de infección de enfermedades transmisibles y permitir, por tanto, un tratamiento precoz.
The prevention and control of communicable disease rely, to a large extent, on effective and early detection approaches. Conventional methods for the detection of a pathogen, such as microbiological culture, are usually time-consuming, laborious, need skilled personnel and are non-amenable to point-of-care diagnostic tools. The development of rapid diagnostic methods in the framework of the ASSURED criteria as (A) Affordable, (SS) Sensitive and Specific, (U) User-friendly, (R) Rapid and Robust, (E) Equipment free, and (D) Deliverable to those who need it, outlined by the World Health Organization (WHO), are under intensive study. Therefore, the present dissertation addresses the design and development of strategies, methods and materials to improve the analytical performance and to simplify the analytical procedure in rapid diagnostic tests, including novel solid-phase preconcentration strategies, amplification methods and advanced materials, as well as their integration in different platforms (mainly biosensors based on electrochemical detection and paper-based strips for optical readout). In all instances, the applications selected are focused on communicable diseases, including foodborne pathogens and mycobacteria. Therefore, two paper-based platforms in different configurations (nucleic acid lateral and vertical flow) are compared in terms of the analytical performance for the detection of Mycobacterium. In order to achieve a further improvement in the limit of detection, the preconcentration of the bacteria is performed by immunomagnetic separation. Secondly, the simultaneous detection of Salmonella and E. coli by nucleic acid lateral flow with visual readout and electrochemical genosensing are evaluated and compared in terms of their analytical performance. Although these methods required double-tagging PCR for amplification, portable, battery-powered thermocyclers can easily be adapted for resource-constrained settings to meet the demands for ASSURED diagnosis. Furthermore, the synthesis of Magnetic Molecularly Imprinted Polymers, in order to replace biological-modified magnetic particles is also presented in this dissertation, taking as a model the detection of biotin and biotinylated molecules with outstanding performance. Moreover, the characterization of the material is performed by different analytical techniques and compared, in all instances, with the non-imprinted polymer. This biomimetic material shows a great potential for the preconcentration and detection of a huge range of analytes. Despite all these progress, nucleic acid amplification techniques are still necessary to reach the challenging limits of detection required in some communicable disease. Isothermal amplification techniques are good candidates to bring sensitive diagnostic tests in places where the PCR can be a barrier. In detail, the electrochemical genosensing of E. coli based on isothermal amplification is also described in this dissertation. In this approach, the electrochemical readout by square-wave voltammetry on disposable electrodes is optimized comparing two different labelling approaches. It is important to highlight that all these strategies aim to be used as tools for the improvement of rapid diagnostic test in low resource settings, to interrupt the chain of infection of communicable diseases and enabling the rapid treatment.
Mitchell, Haydn Thomas. "AN INVESTIGATION OF POLY(N-ISOPROPYLACRYLAMIDE) FOR APPLICATIONS WITH MICROFLUIDIC PAPER-BASED ANALYTICAL DEVICES." DigitalCommons@CalPoly, 2014. https://digitalcommons.calpoly.edu/theses/1248.
Повний текст джерелаMorbioli, Giorgio Gianini. "Funcionalização de celulose para ensaios bioanalíticos em dispositivos microfluídicos baseados em papel (μPADs)." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-23062015-105938/.
Повний текст джерелаFunctionalization of a cellulosic matrix is essential for the success of the paper-based microfluidic analytical devices (µPADs). It allows minimization of sample preparation and user interference, both being major sources of errors in the analytical process. Cellulose oxidation with sodium m-periodate during one hour and the direct chemical immobilization of enzymes on it by Schiff-base (imines) formation, which is made by direct insertion of the enzyme on the oxidized substrate without subsequent steps, is a fast and low cost process of immobilization, presenting great potential of application in paper-based microfluidic analytical devices. The glucose oxidase enzyme immobilized on cellulose, with the addition of trehalose stabilizer presented enhanced catalytic activity - from 31.9 ± 5.5 mmol L-1 for the non-immobilized enzyme to 14.8 ± 2.0 mmol L-1 for the immobilized enzyme with the stabilizing agent - also presenting greater signal homogeneity, which are ideal characteristics in a paper-based rapid test. Wax printing is a simple, inexpensive and fast method by which micro-devices can be fabricated. Additionally, the stacking of layers originating tridimensional devices (3D) allow for the improvement of functionalities of 2-dimensional ones, such as individualized layer treatment and reagent storage at different layers in the same device. Standard addition to analytical curves in paper-based microchips is an alternative to external analytical curves, minimizing handling/sample preparation. The use of 2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid - ABTS redox indicator with the enzymatic reactions and the standard addition method in µPADs presented a good correlation in a growth and saturation Michaelis-Menten model (r2 = 0.8723), in the range of 0 to 10 mmol L-1, and the usage of the linear range to the glucose quantification (0 to 3 mmol L-1) presented a high linear correlation with the estimated concentration from the standard addition curves (r2 = 0.959), showing the potentiality of the method. The coupling of such paper-based devices to automated image analysis software, such as \'PAlizer\', turns the data acquisition process instantaneous, eliminating the need of human intervention during the process, making it more robust, reproducible and rapid. Expectations lie in improving the devices functions and potential so that these low-cost diagnostic devices can one day reach those who need them, contributing significantly to public health.
Braff, Dana. "Technological advancements towards paper-based biomolecular diagnostics." Thesis, 2017. https://hdl.handle.net/2144/27009.
Повний текст джерела"Microfluidic Paper-Based Analytical Devices: Sample Preparation, Medical Monitoring and Diagnostics." 2013.
Знайти повний текст джерелаRodriguez, Natalia Maria. "A paper-based point-of-care molecular diagnostic platform for the developing world." Thesis, 2016. https://hdl.handle.net/2144/14636.
Повний текст джерела(11192533), Kaleb M. Byers. "Platforms and Molecular Mechanisms for Improving Signal Transduction and Signal Enhancement in Multi-step Point-Of-Care Diagnostics." Thesis, 2021.
Знайти повний текст джерелаSwift recognition of disease-causing pathogens at the point-of-care enables life-saving treatment and infection control. However, current rapid diagnostic devices often fail to detect the low concentrations of pathogens present in the early stages of infection, causing delayed and even incorrect treatments. Rapid diagnostics that require multiple steps and/or elevated temperatures to perform have a number of barriers to use at the point-of-care and in the field, and despite efforts to simplify these platforms for ease of use, many still require diagnostic-specific training for the healthcare professionals who use them. Most nucleic acid amplification assays require hours to perform in a sterile laboratory setting that may be still more hours from a patient’s bedside or not at all feasible for transport in remote or low-resourced areas. The cold-chain storage of reagents, multistep sample preparation, and costly instrumentation required to analyze samples has prohibited many nucleic acid detection and antibody-based assays from reaching the point-of-care. There remains a critical need to bring rapid and accessible pathogen identification technologies that determine disease status and ensure effective treatment out of the laboratory.
Paper-based diagnostics have emerged as a portable platform for antigen and nucleic acid detection of pathogens but are often limited by their imperfect control of reagent incubation, multiple complex steps, and inconsistent false positive results. Here, I have developed mechanisms to economically improve thermal incubations, automate dried reagent flow for multistep assays, and specifically detect pathogenic antigens while improving final output sensitivity on paper-based devices. First, I characterize miniaturized inkjet printed joule-heaters (microheaters) that enable thermal control for pathogen lysis and nucleic acid amplification incubation on a low-cost paper-based device. Next, I explore 2-Dimensional Paper Networks as a means to automate multistep visual enhancement reactions with dried reagents to increase the sensitivity and readability of nucleic acid detection with paper-based devices. Lastly, I aim to create a novel Reverse-Transcription Recombinase Polymerase Reaction mechanism to amplify and detect a specific region of the Spike protein domain of SARS-CoV-2. This will allow the rapid detection of SARS-CoV-2 infections to aid in managing the current COVID-19 pandemic. In the future, these tools could be integrated into a rapid diagnostic test for SARS-CoV-2 and other pathogens, ultimately improving the accessibility and sensitivity of rapid diagnostics on multiple fronts.
Kaur, Navjot. "FLIPP-NAAT: A paper-based LAMP assay for point-of-care TB detection." Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5232.
Повний текст джерела(10725807), Jiangshan Wang. "A RAPID PAPER-BASED COLORIMETRIC MOLECULAR TEST FOR SARS-COV-2 POINT-OF-CARE DIAGNOSTIC." Thesis, 2021.
Знайти повний текст джерелаIn the year of 2020, an international pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has afflicted tens of millions of people’s life also disrupting global economics. Diagnostic testing is an important part of ensuring public health until a vaccine that has been shown to be safe and effective is made available to the general public. Most tests for detecting COVID-19 utilize quantitative polymerase chain reaction (qPCR) assays, which is a specific and relatively simple quantitative assay that could provide adequate sensitivity for diagnosing early infection. Although powerful, these lab-based molecular assays have a significant lag time, usually several days before receiving results. To satisfy the needs of different purposes (diagnostics, screening, and surveillance), a unified approach is impractical. This thesis presents an alternative testing method supporting the current procedure of point of care (POC) testing and in community testing. This paper-based test overcomes the limitations of current testing methods by utilizing reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and receiving the result on-site by a color change in the presence of the virus within 60 minutes. The test utilizes untreated freshly collected saliva, a less invasive specimen, as the sample and possesses a limit of detection (LoD) of 200 copies of virus per microliter of whole saliva with an analytical sensitivity of 97% and analytical specificity of 100%. The test requires minimal operator training and could be fabricated on a large-scale using roll-to-roll methods. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostic, etc. simply by changing the specific primers.
Rijo, Catarina Filipa Braz. "A paper-based low-cost label-free biosensing silver core-gold shell nanostructure for SERS to be applied to breast cancer diagnostics." Master's thesis, 2019. http://hdl.handle.net/10362/91955.
Повний текст джерелаWu, Po-Chen, and 吳柏辰. "Paper-Based Diagnostic System." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/v3f4j8.
Повний текст джерела國立臺灣大學
化學工程學研究所
101
Paper-based sensors are rapidly immerging to meet the needs for point-of-care diagnose. Due to its low cost, portable, widely available and easy to operate properties, it has attracted lots of attention in biochemical analysis field. In this work, paper 96 well plate was fabricated via wax printing method to build up a multiple diagnostic system. horseradish peroxidase (HRP enzyme) was conjugated with monoclonal antibody to vascular endothelial grow factor (VEGF antibody), and the quantification of the enzyme was carried out on paper 96 well plate. The influence of temperature and conjugated antibody was also investigated. To test the feasibility using this system for drug screening, HRP-linked VEGF antibody was encapsulated in alginate microspheres via an air pressure driven nozzle and used as a model drug. And the encapsulation efficiency of VEGF antibody was reveal by this paper system. Also, through the recognition of VEGF antibody toward VEGF protein, we perform paper-based ELISA to prove the immuno bioactivity of this system. Another part in our study is to investigate the enzymatic reaction on paper fiber, which is an inhomogeneous condition. Meanwhile, we want to accomplish some common diagnostic trials on paper system. Firstly, glucose and bovine serum albumin (BSA protein) assay was carried out on paper 96 well plate. Afterwards, aspartate transaminase (AST) and alkaline phosphatase (ALP) assay was carried out with different immobilization order. Quantitative analysis was achieved by photographing and processing by imaging software. The Michaelis constant for paper plate and plastic plate was calculated by measuring the initial reaction rate of each group. We observed a 60% increase in Michaelis constant for HRP and glucose oxidase group and no significant difference in ALP group. The quantification of four colorimetric reactions can be successfully carried out on this paper plate system. The result acquired can be distinguished by naked eye and digitalized by imaging software. In addition, the cost for a single trial was highly reduced. With these preliminary data, we believe this paper based system can provide a novel alternative for diagnose purpose in developing countries. And with further modifications, this paper-based system can have a wide potential application in long tern health management, food quality control and environment monitoring.
Shen, Shu-Wei, and 沈書維. "Colorimetric-Based Tuberculosis?Diagnosis Using Gold Nanoparticles?on Paper-Based Diagnostic Devices." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/99565950911230803082.
Повний текст джерела國立中興大學
生醫工程研究所
102
Tuberculosis has always been considered one of the most serious infectious disease, with the advancement of science and medical technology, people continuously find new ways of diagnosing tuberculosis from old theories, but still unable to develop a real-time, convenient and effective diagnostics. In this study, a colorimetric sensing strategy employing gold nanoparticles and a paper assay platform has been developed for tuberculosis diagnosis. Unmodified gold nanoparticles and single-stranded detection oligonucleotides are used to achieve rapid diagnosis, through the theory of DNA hybridization, single-stranded detection oligonucleotides and mycobacterium tuberculosis (MTB) will combine together to become a MTB-detection probe, and the addition of high-concentration sodium chloride solution will induce gold nanoparticles aggregate and make the colloid color change which is regarded as the mechanism to proceed the diagnosis of tuberculosis. This method avoids using complicated and time-consuming thiolated or other surface-modified probe preparation process. Besides combining with paper assay platform can also eliminate the use of sophisticated equipment for data analysis, the color variance for multiple detection results was simultaneously collected and concentrated on cellulose paper with the data readout transmitted for cloud computing via a smartphone. The results show that the 2.6 nM tuberculosis mycobacterium target sequences extracted from patients can easily be detected, and the turnaround time after the human DNA is extracted from clinical samples is approximately 1 hour. We believe the proposed platform possesses the potential for tuberculosis diagnosis monitoring in resource constrained settings.
Fernandes, Alexandra Agostinho Gomes. "An electrochromic paper-based device as a diagnostic test for Cystic Fibrosis." Master's thesis, 2018. http://hdl.handle.net/10362/58114.
Повний текст джерела"RNA-Based Computing Devices for Intracellular and Diagnostic Applications." Doctoral diss., 2019. http://hdl.handle.net/2286/R.I.54869.
Повний текст джерелаDissertation/Thesis
Doctoral Dissertation Biochemistry 2019
"Thermal Actuation and Fluidic Characterization of a Fluorescence-Based Multiplexed Detection System." Master's thesis, 2018. http://hdl.handle.net/2286/R.I.50454.
Повний текст джерелаDissertation/Thesis
Masters Thesis Biomedical Engineering 2018