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Автор: Grafiati
Опубліковано: 6 вересня 2023

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Статті в журналах з теми "Paper-based diagnostics"

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Parolo, Claudio, and Arben Merkoçi. "Paper-based nanobiosensors for diagnostics." Chem. Soc. Rev. 42, no. 2 (2013): 450–57. http://dx.doi.org/10.1039/c2cs35255a.

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2

Parolo, Claudio, and Arben Merkoci. "ChemInform Abstract: Paper-Based Nanobiosensors for Diagnostics." ChemInform 44, no. 20 (April 25, 2013): no. http://dx.doi.org/10.1002/chin.201320276.

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3

Hu, Jie, ShuQi Wang, Lin Wang, Fei Li, Belinda Pingguan-Murphy, Tian Jian Lu, and Feng Xu. "Advances in paper-based point-of-care diagnostics." Biosensors and Bioelectronics 54 (April 2014): 585–97. http://dx.doi.org/10.1016/j.bios.2013.10.075.

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4

Määttänen, Anni, Daniela Fors, Shaoxia Wang, Dimitar Valtakari, Petri Ihalainen, and Jouko Peltonen. "Paper-based planar reaction arrays for printed diagnostics." Sensors and Actuators B: Chemical 160, no. 1 (December 2011): 1404–12. http://dx.doi.org/10.1016/j.snb.2011.09.086.

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5

Wu, Meirong, Qiongyu Lai, Qiang Ju, Lin Li, Hai-Dong Yu, and Wei Huang. "Paper-based fluorogenic devices for in vitro diagnostics." Biosensors and Bioelectronics 102 (April 2018): 256–66. http://dx.doi.org/10.1016/j.bios.2017.11.006.

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Linnes, Jacqueline C., Andy Fan, Natalia M. Rodriguez, Bertrand Lemieux, Huimin Kong, and Catherine M. Klapperich. "Paper-based molecular diagnostic for Chlamydia trachomatis." RSC Adv. 4, no. 80 (2014): 42245–51. http://dx.doi.org/10.1039/c4ra07911f.

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Singh, Saumitra, Mohd Rahil Hasan, Akshay Jain, Roberto Pilloton, and Jagriti Narang. "LFA: The Mysterious Paper-Based Biosensor: A Futuristic Overview." Chemosensors 11, no. 4 (April 19, 2023): 255. http://dx.doi.org/10.3390/chemosensors11040255.

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Анотація:
Lateral flow assay (LFA) is emerging as one of the most popular paper-based biosensors in the field of the diagnostic industry. LFA fills all the gaps between diagnosis and treatment as it provides beneficial qualities to users such as quick response, Point-of-care appeal, early detection, low cost, and effective and sensitive detections of various infectious diseases. These benefits increase LFA’s dependability for disease management because rapid and accurate disease diagnosis is a prerequisite for effective medication. Only 2% of overall healthcare expenditures, according to Roche Molecular Diagnostics, are spent on in vitro diagnostics, even though 60% of treatment choices are based on this data. To make LFA more innovative, futuristic plans have been outlined in many reports. Thus, this review reports on very knowledgeable literature discussing LFA and its development along with recent futuristic plans for LFA-based biosensors that cover all the novel features of the improvement of LFA. LFA might therefore pose a very significant economic success and have a significant influence on medical diagnosis.
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Choi, Jane Ru, Ruihua Tang, ShuQi Wang, Wan Abu Bakar Wan Abas, Belinda Pingguan-Murphy, and Feng Xu. "Paper-based sample-to-answer molecular diagnostic platform for point-of-care diagnostics." Biosensors and Bioelectronics 74 (December 2015): 427–39. http://dx.doi.org/10.1016/j.bios.2015.06.065.

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Syedmoradi, Leila, and Frank A. Gomez. "Paper-based point-of-care testing in disease diagnostics." Bioanalysis 9, no. 11 (June 2017): 841–43. http://dx.doi.org/10.4155/bio-2017-0080.

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Mao, Kang, Xiaocui Min, Hua Zhang, Kuankuan Zhang, Haorui Cao, Yongkun Guo, and Zhugen Yang. "Paper-based microfluidics for rapid diagnostics and drug delivery." Journal of Controlled Release 322 (June 2020): 187–99. http://dx.doi.org/10.1016/j.jconrel.2020.03.010.

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Більше джерел

Дисертації з теми "Paper-based diagnostics"

1

Miller, Eric Alexander. "Development of thermostable affinity reagents for low-cost, paper-based medical diagnostics." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122849.

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Анотація:
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2019
Cataloged from PDF version of thesis.
Includes bibliographical references.
The timely diagnosis and treatment of disease in resource-constrained settings requires the development of robust point-of-care (POC) diagnostics, which provide accurate results and can be employed by users with minimal medical training and limited access to basic infrastructure. One of the most common POC diagnostic formats is the immunochromatographic rapid diagnostic test, which traditionally uses nitrocellulose-immobilized IgG antibodies as binding proteins for the capture of disease biomarkers from patient samples. However, these antibodies are expensive to produce and structurally complex, and are prone to thermal denaturation. In contexts where continuous cold chain storage may be infeasible, the resulting loss in binding activity can manifest as diminished assay sensitivity, leading to adverse clinical outcomes and eroding patient trust in the diagnostic format.
In the interest of replacing diagnostic antibodies with a more cost-effective, robust class of binding proteins, this thesis explores the development of thermostable affinity reagents based on the hyperthermophilic scaffold protein rcSso7d. Given its native microbial host, minimalist structure, and high wild-type melting temperature (98°C), rcSso7d represents a viable alternative to antibodies in in vitro POC assays. To assess the applicability of the rcSso7d scaffold in this context, protein engineering techniques were used to rapidly select analyte-specific binding variants from a yeast surface display library of >109 members. A high-affinity rcSso7d binder was identified, produced in high yield in a bacterial host, and readily purified in a single chromatographic step.
The in vitro activity and thermal stability of this engineered binder were characterized in the context of a low-cost, paper-based assay, and significant improvements in stability and production economics were observed for rcSso7d-based assays, relative to assays featuring a representative antibody reagent. Additionally, general strategies were developed to improve the diagnostic performance of paper-based assays employing rcSso7d-based reagents. In one instance, chimeric protein constructs in which rcSso7d variants are fused to a cellulose-binding domain were found to bind to unmodified cellulose in an oriented fashion and with high efficiency. This substrate anchoring approach permits the rapid, high-density immobilization of the rcSso7d species in paper-based assays, and yields significant sensitivity enhancement by enabling both the depletion of the soluble analyte from the sample, and the processing of large sample volumes within clinically relevant timescales.
Detection reagents incorporating rcSso7d binders were also developed, using novel fusion constructs which enabled in vivo labelling while preserving analyte binding activity. These techniques were applied in the context of a urine-based tuberculosis biomarker, and may one day permit the development of multiplexed assays targeting a suite of these analytes. Such a development would enable point-of-care diagnostic testing without requiring the production of sputa, facilitating disease detection in otherwise inaccessible patient populations (e.g. children under five, the elderly, and immunocompromised patients).
"People who have financially supported this thesis: the NIH Biotechnology Training Program, the Tata Center for Technology and Design, the Deshpande Center, the Sandbox program, and the Singapore- MIT Alliance for Research and Technology"
by Eric Alexander Miller.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Chemical Engineering
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Ward, Jennifer Guerin. "Nitrocellulose Paper Based Microfluidic Platform Development and Surface Functionalization with Anti-IgE Aptamers." DigitalCommons@CalPoly, 2012. https://digitalcommons.calpoly.edu/theses/746.

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Анотація:
The purpose of this thesis project was to demonstrate the ability to utilize the capabilities of aptamers so that they may act as capture reagents for paper microfluidic devices. Several characterization experiments were conducted as a precursor before the final experimentation was performed. Paper characterization, manufacturing protocols for printing and heating, as well as 3D chip fabrication were all performed and analyzed. The results confirmed that the control of fluid through a 3D microfluidic device based in nitrocellulose is possible. For the biochemistry portion of this thesis report, antibodies and aptamers were chosen to react with IgE, an antibody that is present in high concentrations in the urine of patients diagnosed with respiratory distress. Antibody chips were successfully created as a baseline lateral flow assay for comparison to new aptamer detector reagents. The aptamer experiments were able to demonstrate that it is possible to utilize the capabilities of aptamers so that they may behave as capture reagents in paper microfluidic devices. Overall, the experiments performed were extremely supportive of the ability to develop the field of paper microfluidics with the use of aptamers so that patient populations across the globe can be more accurately and effectively diagnosed.
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Lama, Lara. "Novel methods for improving rapid paper-based protein assays with gold nanoparticle detection." Licentiate thesis, KTH, Proteomik och nanobioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-214065.

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Анотація:
This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray assays. The assays utilize the optical characteristics of colloidal gold nanoparticles for the colorimetric detection of proteins. Multiplexed detection in sandwich immunoassays is limited by cross-reactivity between different detection antibodies. The cross-reactivity between antibodies can contribute to increased background noise - decreasing the Limit-of-Detection of the assay - or generate false positive signals. Paper I shows improved assay sensitivity in a multiplexed vertical flow assay by the application of ultrasonic energy to the gold nanoparticles functionalized with detection antibodies. The ultrasonication of the antibody conjugated gold nanoparticles resulted in a 10 000 fold increase in sensitivity in a 3-plex assay. COMSOL Multiphysics was used to simulate the acoustical energy of the probe used in Paper I for obtaining an indication of the size and direction of the forces acting upon the functionalized gold nanoparticles. In Paper II, it was studied if different gold nanoparticle conjugation methods and colorimetric signal enhancement of the gold nanoparticle conjugates could influence the sensitivity of a paper-based lateral flow microarray assay, targeting cardiac troponin T for the rapid diagnostics of acute myocardial infarction. Ultrasonication and signal enhancement of the detection gold nanoparticles has the potential of improving the sensitivity of paper based assays and expanding their potential future applications.

QC 20170911

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Glavan, Ana. "Chemical Approaches to the Surface Engineering of Paper and Cellulose-Based Materials for Microfluidics, Electronics and Low-Cost Diagnostics." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:26718749.

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Анотація:
Paper (and other cellulose-based materials such as cotton thread and fabrics) are underexploited as materials for the construction of “high-tech” and “lab-on-a-chip” devices. One major drawback of paper is its tendency to absorb water from the environment and, with wetting, to change its mechanical properties; other challenges relate to control over the attachment of molecules (e.g. antibodies, DNA) and cells on its surface, and to the addition of electronic function. The goal of this thesis is to develop paper as a substrate for a range of applications— microfluidics, substrates for electronic systems and MEMS, low-cost diagnostics, cell biology, and optics. The approach involves chemically modifying the surface of the paper to provide new functions without altering any of its defining properties: mechanical flexibility, foldability, light weight, gas permeability, and low cost. The first part of my thesis describes the modification of paper by silanization with organosilanes such as alkyl- and fluoroalkyl trichlorosilanes in the gas phase. Here, silanization is used to lower the surface free energy of the paper and to minimize the tendency of paper to absorb liquids and vapors, and especially water. Chapter 1 and Appendix 3 demonstrate that the combination of long fluoroalkyl chains of grafted siloxanes with the micro-scale roughness and porosity of paper yielded a material that is omniphobic (both hydrophobic and oleophobic), while preserving the properties of mechanical flexibility and low resistance to transport of gas of the untreated paper. Appendix 3 shows that features of omniphobic paper can be used to construct microtiter plates and liquid-filled gas sensors using standard paper folding techniques, while Appendix 4 shows that new type of microfluidic device fabricated by carving microchannels into the surface of omniphobic paper. The resulting devices have open, unobstructed channels (with dimensions as small as 45 μm) and thus exhibit fluid dynamics similar to conventional PDMS-based microfluidics, but are much lighter and have the potential to be much less expensive than PDMS-based devices. The second part of my thesis is focused on engineering the surface of paper to enable efficient immobilization of capture and target molecules for bioanalysis. In one approach, described in Appendix 5, we exploit the ease with which the surface chemistry of paper (i.e. the surface of the cellulose fibers making up the paper) can be modified, in order to enhance the immobilization of antibodies and antigens on the surface of the paper via hydrophobic interactions, while preventing the wicking of the fluids into the paper substrate. As an application in low-cost diagnostics, we describe a low-cost electrochemical device for ELISA intended for use in resource-limited settings. In a second approach, described in Chapter 2, we developed of an efficient procedure for assembling microarrays of ssDNA and proteins on paper, at the lowest practical cost. This method starts with the synthesis of DNA oligonucleotides covalently linked to paper, and proceeds to generate ssDNA arrays that, through hybridization with complementary strands of DNA, are capable of simultaneously capturing DNA, DNA-conjugated protein antigens, and DNA-conjugated antibodies. The third part of my thesis describes the simple, inexpensive fabrication of electrodes for paper-based electrochemical systems. A first method describes, in Appendix 6, the development of inkjet printing as a method for high resolution printing of conductive patterns on omniphobic “RF” paper, both to extend its promise as a substrate for paper electronics, and to enable us to integrate it into our program in low-cost, paper based diagnostics. A second method, described in Chapter 3, circumvents the need for printing, and instead focuses on the fabrication and reconfiguration of simple, versatile, and inexpensive electroanalytical devices in which conventional stainless-steel pins—in unmodified form or after coating with a carbon paste—are used as electrodes.
Chemistry and Chemical Biology
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5

Katis, Ioannis. "Laser direct write techniques for the fabrication of paper-based diagnostic devices." Thesis, University of Southampton, 2015. https://eprints.soton.ac.uk/388397/.

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Анотація:
We report on the use of laser direct-write techniques for the fabrication of point-of-care paper-based diagnostic sensors. These include laser-based deposition, laser ablation and laser-induced photo-polymerisation. Firstly, Laser Induced Forward Transfer (LIFT) was employed to deposit biomolecules from a donor film onto paper receivers. Paper was chosen as the ideal receiver because of its inherent properties which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged antibodies were LIFT-printed and their viability was confirmed via a colorimetric enzyme-linked immunosorbent assay (ELISA). Secondly, we report on the laser-based structuring of paper-based fluidic devices. Laser-scanning the paper defines the areas that will be polymerised, thus creating barriers that keep the liquid solutions contained. Complicated devices are easy to fabricate and the flexibility of this technique allows for unique patterns, making it appropriate for rapid prototyping but also for large-scale production. Furthermore, the laser patterning technique allows control of the depth or degree of polymerisation, thereby allowing the liquid to wick through but also imposition of flow delays. Finally, the use of lasers for the fabrication of a 'master' which can be used for casting a PDMS mould for applications in micro-contact printing. The combination of the above mentioned techniques represent the platform technology for the rapid, precise and versatile laser-based fabrication of diagnostic point-of-care sensors.
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Tageson, Mackenzie Elizabeth. "FUNCTIONAL 3-D CELLULOSE & NITROCELLULOSE PAPER-BASED, MULITPLEX DIAGNOSTIC PLATFORMS WITHOUT COUPLING AGENTS." DigitalCommons@CalPoly, 2013. https://digitalcommons.calpoly.edu/theses/1128.

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Анотація:
The purpose of this thesis was to demonstrate device functionality of 3-D paper-based, multiplex platforms, µPADs, without the use of coupling agents between layers. Previously, these platforms were fabricated with double-sided tape and cellulose powder to try to augment proper fluid routing, but difficulties with this method occurred. An acrylic housing unit with strategically placed pressure tabs was designed to aid horizontal and vertical fluid routing through the platform, thus eliminating the inconsistencies associated with coupling agents. Channel characterization studies, a COMSOLTM simulation, and development time studies were performed to aid device design and demonstrate device functionality. The implementation of this µPAD platform as a diagnostic instrument was validated via lateral flow immunoassays utilizing both biotinylated antibodies and biotinylated aptamers as capture reagents. Successful detection of the target analyte, IgE, as well as successful fluid routing through multiple layers of membrane was demonstrated by immunoassays performed on 3-D, multiplex platforms. Another important result determined the aptamers’ ability to detect IgE to be statistically the same as the antibodies’ ability; thus confirming aptamers as viable capture reagent alternatives to antibodies in lateral flow assays. Overall, this research project was performed to develop and validate via experiment a prototype paper-based microfluidic diagnostic device, µPAD, with the capability to detect multiple biomarkers on one platform.
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Ben, Aissa Soler Alejandra. "Rapid diagnostic test for the detection of communicable diseases." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2020. http://hdl.handle.net/10803/670392.

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Анотація:
La prevenció i el control de les malalties transmissibles depenen, en gran mesura, de la detecció ràpida i eficaç. Els mètodes convencionals per a la detecció d'un patogen, com ara el cultiu microbiològic, generalment requereixen molt de temps, són laboriosos, necessiten personal qualificat i no són aptes com a eines de diagnòstic en el punt d'atenció. El desenvolupament de mètodes de diagnòstic ràpid en el marc dels criteris ASSURED, de l'anglès (A) Affordable, (SS) Sensitive i Didàctiques, (O) User-friendly, (R) Rapid and Robust, (I) Equipment free, and (d) Deliverable to those who need it, Affordable, descrits per l'Organització Mundial de la Salut (OMS), es troben en l'actualitat sota intens estudi. Per tant, la present tesi aborda el disseny i desenvolupament d'estratègies, mètodes i materials per millorar les prestacions analítiques i simplificar el procediment en proves de diagnòstic ràpid, incloses noves estratègies de preconcentració en fase sòlida, mètodes d'amplificació i materials avançats, així com la seva integració en diferents plataformes (principalment biosensors basats en detecció electroquímica i proves en paper amb lectura òptica). En tots els casos, les aplicacions seleccionades es centren en malalties transmissibles, inclosos els patògens transmesos pels aliments i els micobacteris. Amb aquesta finalitat, es comparen dues plataformes basades en paper en diferents configuracions (flux lateral i vertical) en termes de rendiment analític per a la detecció de Mycobacterium. Per aconseguir una millora addicional en el límit de detecció, s'estudia la preconcentració prèvia dels bacteris per separació immunomagnètica. En segon lloc, s'avaluen i es comparen en termes del seu rendiment analític la detecció simultània de Salmonella i E. coli mitjançant flux lateral d'àcid nucleic amb lectura visual i genosensors electroquímics. Si bé aquests mètodes requereixen PCR de doble etiquetatge per a l'amplificació, es poden adaptar fàcilment a termocicladors portàtils que funcionen amb bateries per poder ser realitzats en entorns amb recursos limitats per satisfer les demandes de diagnòstic ASSURED. A més, també es presenta en aquesta tesi la síntesi de polímers magnètics impresos molecularment, per tal de reemplaçar les partícules magnètiques biològicament modificades, i prenent com a model la detecció de biotina i de molècules biotinilades. A més, es realitza la caracterització del material mitjançant diferents tècniques analítiques i es compara, en tots els casos, amb el polímer no imprès. Aquest material biomimètic mostra un gran potencial per a la preconcentració i detecció d'una àmplia gamma d'analits. Malgrat tot els progressos, les tècniques d'amplificació d'àcid nucleic segueixen essent necessàries per assolir els límits de detecció requerits en algunes malalties transmissibles. En aquest sentit, les tècniques d'amplificació isotèrmiques són bons candidats per dur a terme proves de diagnòstic en entorns on la PCR pot ser una barrera. En concret, es descriu en aquest treball la detecció d' E.coli mitjançant un genosensor electroquímic basat en l'amplificació isotèrmica. En aquest cas, s'optimitza la lectura electroquímica per voltamperometria d'ona quadrada en elèctrodes d'un sol ús comparant dues estratègies de marcatge del producte amplificat. És important ressaltar que totes aquestes estratègies apunten a ser utilitzades com a eines per millorar les proves de diagnòstic ràpid en entorns de baixos recursos, per interrompre la cadena d'infecció de malalties transmissibles i permetre, per tant, un tractament precoç.
La prevención y el control de las enfermedades transmisibles dependen, en gran medida, de la detección rápida y eficaz. Los métodos convencionales para la detección de un patógeno, como el cultivo microbiológico, generalmente requieren mucho tiempo, son laboriosos, necesitan personal cualificado y no son aptos como herramientas de diagnóstico en el punto de atención. El desarrollo de métodos de diagnóstico rápido en el marco de los criterios ASSURED, del inglés (A) Affordable, (SS) Sensitive and Specific, (U) User-friendly, (R) Rapid and Robust, (E) Equipment free, and (D) Deliverable to those who need it, Affordable, descritos por la Organización Mundial de la Salud (OMS), se encuentran en la actualidad bajo intenso estudio. Por lo tanto, la presente tesis aborda el diseño y desarrollo de estrategias, métodos y materiales para mejorar las prestaciones analíticas y simplificar el procedimiento en pruebas de diagnóstico rápido, incluidas nuevas estrategias de preconcentración en fase sólida, métodos de amplificación y materiales avanzados, así como su integración en diferentes plataformas (principalmente biosensores basados en detección electroquímica y pruebas en papel con lectura óptica). En todos los casos, las aplicaciones seleccionadas se centran en enfermedades transmisibles, incluidos los patógenos transmitidas por los alimentos y las micobacterias. Con este fin, se comparan dos plataformas basadas en papel en diferentes configuraciones (flujo lateral y vertical) en términos del rendimiento analítico para la detección de Mycobacterium. Para lograr una mejora adicional en el límite de detección, se estudia la preconcentración previa de las bacterias por separación inmunomagnética. En segundo lugar, se evalúan y se comparan en términos de su rendimiento analítico la detección simultánea de Salmonella y E. coli mediante flujo lateral de ácido nucleico con lectura visual y genosensores electroquímicos. Si bien estos métodos requieren PCR de doble etiquetado para la amplificación, se pueden adaptar fácilmente a termocicladores portátiles que funcionan con baterías para poder ser realizados en entornos con recursos limitados para satisfacer las demandas de diagnóstico ASSURED. Además, también se presenta en esta disertación la síntesis de polímeros magnéticos impresos molecularmente, con el objeto de reemplazar las partículas magnéticas biológicamente modificadas, y tomando como modelo la detección de biotina y moléculas biotiniladas. Además, se realiza la caracterización del material mediante diferentes técnicas analíticas y se compara, en todos los casos, con el polímero no impreso. Este material biomimético muestra un gran potencial para la preconcentración y detección de una amplia gama de analitos. A pesar de todo este progreso, las técnicas de amplificación de ácido nucleico siguen siendo necesarias para alcanzar los límites de detección requeridos en algunas enfermedades transmisibles. Las técnicas de amplificación isotérmica son buenos candidatos para llevar pruebas de diagnóstico en entornos donde la PCR puede ser una barrera. En concreto, se describe en esta disertación la detección de E. coli mediante un genosensor electroquímico basada en la amplificación isotérmica. En este caso, se optimiza la lectura electroquímica por voltamperometría de onda cuadrada en electrodos desechables comparando dos estrategias de marcaje del producto amplificado. Es importante resaltar que todas estas estrategias apuntan a ser utilizadas como herramientas para mejorar las pruebas de diagnóstico rápido en entornos de bajos recursos, para interrumpir la cadena de infección de enfermedades transmisibles y permitir, por tanto, un tratamiento precoz.
The prevention and control of communicable disease rely, to a large extent, on effective and early detection approaches. Conventional methods for the detection of a pathogen, such as microbiological culture, are usually time-consuming, laborious, need skilled personnel and are non-amenable to point-of-care diagnostic tools. The development of rapid diagnostic methods in the framework of the ASSURED criteria as (A) Affordable, (SS) Sensitive and Specific, (U) User-friendly, (R) Rapid and Robust, (E) Equipment free, and (D) Deliverable to those who need it, outlined by the World Health Organization (WHO), are under intensive study. Therefore, the present dissertation addresses the design and development of strategies, methods and materials to improve the analytical performance and to simplify the analytical procedure in rapid diagnostic tests, including novel solid-phase preconcentration strategies, amplification methods and advanced materials, as well as their integration in different platforms (mainly biosensors based on electrochemical detection and paper-based strips for optical readout). In all instances, the applications selected are focused on communicable diseases, including foodborne pathogens and mycobacteria. Therefore, two paper-based platforms in different configurations (nucleic acid lateral and vertical flow) are compared in terms of the analytical performance for the detection of Mycobacterium. In order to achieve a further improvement in the limit of detection, the preconcentration of the bacteria is performed by immunomagnetic separation. Secondly, the simultaneous detection of Salmonella and E. coli by nucleic acid lateral flow with visual readout and electrochemical genosensing are evaluated and compared in terms of their analytical performance. Although these methods required double-tagging PCR for amplification, portable, battery-powered thermocyclers can easily be adapted for resource-constrained settings to meet the demands for ASSURED diagnosis. Furthermore, the synthesis of Magnetic Molecularly Imprinted Polymers, in order to replace biological-modified magnetic particles is also presented in this dissertation, taking as a model the detection of biotin and biotinylated molecules with outstanding performance. Moreover, the characterization of the material is performed by different analytical techniques and compared, in all instances, with the non-imprinted polymer. This biomimetic material shows a great potential for the preconcentration and detection of a huge range of analytes. Despite all these progress, nucleic acid amplification techniques are still necessary to reach the challenging limits of detection required in some communicable disease. Isothermal amplification techniques are good candidates to bring sensitive diagnostic tests in places where the PCR can be a barrier. In detail, the electrochemical genosensing of E. coli based on isothermal amplification is also described in this dissertation. In this approach, the electrochemical readout by square-wave voltammetry on disposable electrodes is optimized comparing two different labelling approaches. It is important to highlight that all these strategies aim to be used as tools for the improvement of rapid diagnostic test in low resource settings, to interrupt the chain of infection of communicable diseases and enabling the rapid treatment.
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Mitchell, Haydn Thomas. "AN INVESTIGATION OF POLY(N-ISOPROPYLACRYLAMIDE) FOR APPLICATIONS WITH MICROFLUIDIC PAPER-BASED ANALYTICAL DEVICES." DigitalCommons@CalPoly, 2014. https://digitalcommons.calpoly.edu/theses/1248.

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N,N′-methylenebisacrylamide-crosslinked poly(N-isopropylacrylamide), also known as P(NIPAM), was developed as a fluid delivery system for use with microfluidic paper-based analytical devices (microPADs). MicroPADs are postage-stamp-sized devices made out of paper that can be used as platforms for low-cost, simple-to-use point-of-care diagnostic assays. P(NIPAM) is a thermally responsive polymer that absorbs aqueous solutions at room temperature and will expel the solutions to microPADs when heated. The fluid delivery characteristics of P(NIPAM) were assessed, and P(NIPAM) was able to deliver multiple solutions to microPADs in specific sequences or simultaneously in a laminar-flow configuration. P(NIPAM) was then shown to be suitable for delivering four classes of reagents to microPADs: small molecules, enzymes, antibodies and DNA. P(NIPAM) successfully delivered a series of standard concentrations of glucose (0 – 5 mM) to microPADs equipped to perform a colorimetric glucose assay. The results of these tests were used to produce an external calibration curve, which in turn was used to determine accurately the concentrations of glucose in sample solutions. P(NIPAM) successfully delivered fluorescein-labeled IgG and fluorescein-labeled oligonucleotides (20 base pairs) to microPADs in a variety of concentrations. P(NIPAM) also successfully delivered horseradish peroxidase (HRP) to microPADs, and it was determined that HRP could be stored in P(NIPAM) for 35 days with minimal loss in activity. The combination of P(NIPAM) with microPADs will allow for more complex assays to be performed with minimal user input, will facilitate the preparation of external calibration curves in the field, and may be useful in extending the shelf life of microPADs by stabilizing reagents.
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Morbioli, Giorgio Gianini. "Funcionalização de celulose para ensaios bioanalíticos em dispositivos microfluídicos baseados em papel (μPADs)." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-23062015-105938/.

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A funcionalização da matriz celulósica é um ponto essencial para o aprimoramento dos dispositivos microfluídicos baseados em papel (µPADs). Ela permite minimizar o preparo de amostras e a interferência do usuário, principais fontes de erro no processo analítico. A oxidação da celulose durante uma hora com m-periodato de sódio e a imobilização química de enzimas a partir da formação de bases de Schiff (iminas), via a adição direta da enzima ao substrato oxidado sem a necessidade de outras etapas, é um processo rápido e de baixo custo, apresentando grande potencialidade de aplicação nos dispositivos microfluídicos em papel. A enzima glicose oxidase imobilizada na celulose, com a adição do estabilizante trealose, apresentou elevada atividade catalítica - de 31,9 ± 5,5 mmol L-1 para a enzima não imobilizada a 14,8 ± 2,0 mmol L-1 para a enzima imobilizada e com o estabilizante - além de apresentar maior homogeneidade de sinal, condições desejáveis em testes rápidos em papel. A confecção de dispositivos em papel via impressão em cera alia rapidez e baixo custo de produção, e o arranjo em camadas para originar dispositivos tridimensionais (3D) permite ampliar as funcionalidades dos dispositivos em duas dimensões, tal como o tratamento individualizado de camadas e o armazenamento de reagentes no próprio dispositivo. O método da adição de padrão para obtenção de curvas analíticas no próprio microchip em papel surge como alternativa às curvas analíticas externas, minimizando a manipulação e o preparo de amostras. O uso do ácido 2,2’-azino-bis (3-etilbenzotiazolina-6-sulfônico) - ABTS como indicador redox para as reações enzimáticas e o método de adição de padrão nos µPADs apresentou boa correlação com um modelo de crescimento e saturação de Michaelis-Menten (r2 = 0,8723) na faixa de 0 a 10 mmol L-1, e a utilização da faixa linear para quantificação de glicose (0 a 3 mmol L-1) apresentou grande correlação linear com a concentração estimada pelas curvas de adição de padrão (r2 = 0,959), demonstrando a potencialidade do método. A união da tecnologia desses dispositivos em papel com a de um software automatizado de reconhecimento de imagens (PAlizer) torna instantânea a obtenção de resultados, eliminando-se a necessidade de intervenção humana no processo, tornando os testes em papel mais robustos, reprodutíveis e rápidos. Com o contínuo aperfeiçoamento das funcionalidades e potencialidades dos dispositivos microfluídicos em papel espera-se que os testes diagnósticos de baixo custo atinjam àqueles que deles necessitam, contribuindo para a saúde da população.
Functionalization of a cellulosic matrix is essential for the success of the paper-based microfluidic analytical devices (µPADs). It allows minimization of sample preparation and user interference, both being major sources of errors in the analytical process. Cellulose oxidation with sodium m-periodate during one hour and the direct chemical immobilization of enzymes on it by Schiff-base (imines) formation, which is made by direct insertion of the enzyme on the oxidized substrate without subsequent steps, is a fast and low cost process of immobilization, presenting great potential of application in paper-based microfluidic analytical devices. The glucose oxidase enzyme immobilized on cellulose, with the addition of trehalose stabilizer presented enhanced catalytic activity - from 31.9 ± 5.5 mmol L-1 for the non-immobilized enzyme to 14.8 ± 2.0 mmol L-1 for the immobilized enzyme with the stabilizing agent - also presenting greater signal homogeneity, which are ideal characteristics in a paper-based rapid test. Wax printing is a simple, inexpensive and fast method by which micro-devices can be fabricated. Additionally, the stacking of layers originating tridimensional devices (3D) allow for the improvement of functionalities of 2-dimensional ones, such as individualized layer treatment and reagent storage at different layers in the same device. Standard addition to analytical curves in paper-based microchips is an alternative to external analytical curves, minimizing handling/sample preparation. The use of 2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid - ABTS redox indicator with the enzymatic reactions and the standard addition method in µPADs presented a good correlation in a growth and saturation Michaelis-Menten model (r2 = 0.8723), in the range of 0 to 10 mmol L-1, and the usage of the linear range to the glucose quantification (0 to 3 mmol L-1) presented a high linear correlation with the estimated concentration from the standard addition curves (r2 = 0.959), showing the potentiality of the method. The coupling of such paper-based devices to automated image analysis software, such as \'PAlizer\', turns the data acquisition process instantaneous, eliminating the need of human intervention during the process, making it more robust, reproducible and rapid. Expectations lie in improving the devices functions and potential so that these low-cost diagnostic devices can one day reach those who need them, contributing significantly to public health.
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Braff, Dana. "Technological advancements towards paper-based biomolecular diagnostics." Thesis, 2017. https://hdl.handle.net/2144/27009.

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Clinically tractable diagnostics must be low-cost, rapid, sensitive, easy to use, and adaptable to new targets. With its rational design, synthetic biology holds promise for developing diagnostic technologies that can address these needs. In particular, progress in synthetic biology has led to improved circuit-building abilities and a large collection of biomolecular sensors. However, these technologies fundamentally require transcription and translation, limiting their applicability to cellular contexts In vitro cell-free expression systems that contain transcription and translation machinery provide the environment necessary for biologically-based technologies to function independently of living cells. Our lab recently developed a paper-based system for cell-free gene expression, which utilizes cell-free extracts that are freeze-dried on to paper and other porous substrates to allow for long-term preservation of synthetic circuits at room temperature. Our platform represents a scalable, cost-effective technology that is easy to use and is compatible with synthetic biology tools. In this dissertation, I present several advancements to this diagnostic platform that are geared towards improving the system’s clinical tractability. In the context of developing a diagnostic for Zika virus that could be deployed in low-resource settings, I demonstrate improvements to diagnostic sensitivity and rapid sample processing that allow for detection of low femtomolar quantities of active virus directly from blood plasma samples. I also describe preliminary results towards a streamlined one-pot amplification-sensing reaction, and propose the development of a paper-based diagnostic for antibiotic susceptibility testing.
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Книги з теми "Paper-based diagnostics"

1

Land, Kevin J., ed. Paper-based Diagnostics. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-96870-4.

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Lee, Jeong Hoon, ed. Paper-Based Medical Diagnostic Devices. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8723-8.

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Land, Kevin J. Paper-based Diagnostics: Current Status and Future Applications. Springer, 2018.

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4

Paper-Based Analytical Devices for Chemical Analysis and Diagnostics. Elsevier, 2021.

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Paper-based Analytical Devices for Chemical Analysis and Diagnostics. Elsevier, 2022. http://dx.doi.org/10.1016/c2019-0-01488-4.

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Thiago R. L. C. Paixao and William R. Araujo. Paper-Based Analytical Devices for Chemical Analysis and Diagnostics. Elsevier, 2021.

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7

Lee, Jeong-Hoon. Paper-Based Medical Diagnostic Devices: As a Part of Bioanalysis-Advanced Materials, Methods, and Devices. Springer Singapore Pte. Limited, 2020.

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8

Lee, Jeong-Hoon. Paper-Based Medical Diagnostic Devices: As a Part of Bioanalysis-Advanced Materials, Methods, and Devices. Springer Singapore Pte. Limited, 2021.

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9

Jolly, Elaine, Andrew Fry, and Afzal Chaudhry, eds. Epidemiology and evidence-based medicine. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199230457.003.0020.

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Chapter 20 focuses on epidemiology and evidence-based medicine. It covers study design, types of data and descriptive statistics, from samples to populations, relationships, relative risk, odds ratios, and 'number needed to treat', survival analysis, sample size, diagnostic tests, meta-analysis, before concluding with advice on how to read a paper.
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Shipley, Mike. The definition of fibromyalgia. Edited by Paul Farquhar-Smith, Pierre Beaulieu, and Sian Jagger. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780198834359.003.0049.

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The landmark paper discussed in this chapter is ‘Primary fibromyalgia (fibrositis): Clinical study of 50 patients with matched normal controls’, published by Yunus et al. in 1981. Over the years, long-term pain has had a confusing variety of names and, to some extent, that confusion persists. Chronic pain can be defined as pain which persists beyond the time expected for healing. Yunus et al. were the first to define fibromyalgia (fibrositis), as they called it, developing evidence-based diagnostic guidelines by comparing a group of patients with a pain-free matched control group. Based on their work, they also suggested a series of pain management approaches which remain valid today.
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Частини книг з теми "Paper-based diagnostics"

1

Land, Kevin J., Suzanne Smith, and Rosanna W. Peeling. "Unmet Diagnostics Needs for the Developing World." In Paper-based Diagnostics, 1–21. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_1.

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Schabel, Samuel, and Markus Biesalski. "The Role of Paper Chemistry and Paper Manufacture in the Design of Paper-Based Diagnostics." In Paper-based Diagnostics, 23–46. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_2.

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Channon, Robert B., Monpichar Srisa-Art, Katherine Boehle, and Charles Henry. "Critical Components and Innovations in Paper-Based Analytical Devices." In Paper-based Diagnostics, 47–87. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_3.

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Morbioli, Giorgio Gianini, Thiago Mazzu-Nascimento, Amanda M. Stockton, and Emanuel Carrilho. "How Are These Devices Manufactured?" In Paper-based Diagnostics, 89–122. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_4.

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Smith, Suzanne, Dario Mager, Jan G. Korvink, and Kevin J. Land. "Printed Functionalities on Paper Substrates Towards Fulfilment of the ASSURED Criteria." In Paper-based Diagnostics, 123–70. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_5.

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Karlikow, Margot, and Keith Pardee. "The Many Roads to an Ideal Paper-based Device." In Paper-based Diagnostics, 171–201. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_6.

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Korvink, Jan G. "The Fascination of Paper." In Paper-based Diagnostics, 203–14. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96870-4_7.

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Batule, Bhagwan S., Youngung Seok, and Min-Gon Kim. "Paper-Based Molecular Diagnostics." In Bioanalysis, 155–81. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-8723-8_8.

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Akram, Muhammad Safwan, Ronan Daly, Fernando da Cruz Vasconcellos, Ali Kemal Yetisen, Ian Hutchings, and Elizabeth A. H. Hall. "Applications of Paper-Based Diagnostics." In Lab-on-a-Chip Devices and Micro-Total Analysis Systems, 161–95. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-08687-3_7.

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Phillips, Scott T., and Nicole K. Thom. "Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays." In Microfluidic Diagnostics, 185–96. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-134-9_13.

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Тези доповідей конференцій з теми "Paper-based diagnostics"

1

Jaitpal, Siddhant, Suhash Chavva, and Samuel Mabbott. "Towards point-of-care detection of microRNAs using paper-based microfluidics." In Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2021. http://dx.doi.org/10.1117/12.2589180.

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Rourke, Anna S., Alec B. Walter, Braden Carroll, Anita M. Mahadevan-Jansen, and Andrea K. Locke. "A novel paper-based substrate for coffee ring augmented surface-enhanced Raman spectroscopy." In Optical Diagnostics and Sensing XXII: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2022. http://dx.doi.org/10.1117/12.2609871.

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Layouni, Rabeb, Juliana Yang, Simon J. Ward, Paul E. Laibinis, and Sharon M. Weiss. "Paper-based diagnostics incorporating porous silicon (Conference Presentation)." In Frontiers in Biological Detection: From Nanosensors to Systems XV, edited by Benjamin L. Miller, Sharon M. Weiss, and Amos Danielli. SPIE, 2023. http://dx.doi.org/10.1117/12.2653126.

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Esteves, Matheus, Jaione Tirapu-Azpiroz, Daniel Vitor Lopes Marcondes Marçal, Ademir Ferreira Silva, Ricardo Ohta, and Mathias Steiner. "Illumination compensation algorithm for colorimetric detection of microfluidic paper-based devices with a smartphone." In Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2021. http://dx.doi.org/10.1117/12.2578441.

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Chao, Gu, Zhu Mengzhao, Zhu Wenbing, Jian Hao, and Runhao Zou. "Thermal Ageing Condition Assessment for Transformer Using Natural Ester-Paper Insulation Based on Polarization and Depolarization Current." In 2018 International Conference on Diagnostics in Electrical Engineering (Diagnostika). IEEE, 2018. http://dx.doi.org/10.1109/diagnostika.2018.8526122.

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6

Mostafalu, Pooria, and Sameer Sonkusale. "Paper-based super-capacitor using micro and nano particle deposition for paper-based diagnostics." In 2013 IEEE Sensors. IEEE, 2013. http://dx.doi.org/10.1109/icsens.2013.6688437.

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Belsare, Sayali, and Gerard Coté. "Development of paper-based colorimetric assays for monitoring gestational diabetes at the point-of-care." In Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2021. http://dx.doi.org/10.1117/12.2585542.

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Hamann, Hendrik F. "Antenna-based near-field scanning optical microscopy (Invited Paper)." In Nondestructive Evaulation for Health Monitoring and Diagnostics, edited by Robert E. Geer, Norbert Meyendorf, George Y. Baaklini, and Bernd Michel. SPIE, 2005. http://dx.doi.org/10.1117/12.607421.

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Locke, Andrea K., Gerard L. Coté, and Nicolaas E. P. Deutz. "Development of a paper-based vertical flow SERS assay for citrulline detection using aptamer-conjugated gold nanoparticles." In Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2018. http://dx.doi.org/10.1117/12.2290604.

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Bezdek, Zdenek. "Porous tapes for VPI insulation systems of high voltage stator windings of large rotating electrical machines based on the mica paper with a large surface weight." In 2016 Conference on Diagnostics in Electrical Engineering (Diagnostika). IEEE, 2016. http://dx.doi.org/10.1109/diagnostika.2016.7736471.

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Звіти організацій з теми "Paper-based diagnostics"

1

Kolgatin, Oleksandr H., Larisa S. Kolgatina, Nadiia S. Ponomareva, and Ekaterina O. Shmeltser. Systematicity of students’ independent work in cloud learning environment. [б. в.], September 2019. http://dx.doi.org/10.31812/123456789/3247.

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The paper deals with the problem of out-of-class students’ independent work in information and communication learning environment based on cloud technologies. Results of appropriate survey among students of pedagogical university are discussed. The students answered the questions about systematicity of their learning activity and propositions for its improving. It is determined that the leading problems are needs in more careful instruction according to features of the task completing, insufficient experience in self-management, the lack of internal motivation. Most of all, students recommend to provide the tasks with detail instruction (oral or written) and to pay attention to careful planning the time that is necessary for full completion of the task. It is pointed that such complicated requirements can be satisfied only by complex use of information and communication technologies as well as the automated system of pedagogical diagnostics. Some requirements for management of students’ out-of-classroom independent work are formulated as a result of this discussion.
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2

Paule, Bernard, Flourentzos Flourentzou, Tristan de KERCHOVE d’EXAERDE, Julien BOUTILLIER, and Nicolo Ferrari. PRELUDE Roadmap for Building Renovation: set of rules for renovation actions to optimize building energy performance. Department of the Built Environment, 2023. http://dx.doi.org/10.54337/aau541614638.

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In the context of climate change and the environmental and energy constraints we face, it is essential to develop methods to encourage the implementation of efficient solutions for building renovation. One of the objectives of the European PRELUDE project [1] is to develop a "Building Renovation Roadmap"(BRR) aimed at facilitating decision-making to foster the most efficient refurbishment actions, the implementation of innovative solutions and the promotion of renewable energy sources in the renovation process of existing buildings. In this context, Estia is working on the development of inference rules that will make it possible. On the basis of a diagnosis such as the Energy Performance Certificate, it will help establishing a list of priority actions. The dynamics that drive this project permit to decrease the subjectivity of a human decisions making scheme. While simulation generates digital technical data, interpretation requires the translation of this data into natural language. The purpose is to automate the translation of the results to provide advice and facilitate decision-making. In medicine, the diagnostic phase is a process by which a disease is identified by its symptoms. Similarly, the idea of the process is to target the faulty elements potentially responsible for poor performance and to propose remedial solutions. The system is based on the development of fuzzy logic rules [2],[3]. This choice was made to be able to manipulate notions of membership with truth levels between 0 and 1, and to deliver messages in a linguistic form, understandable by non-specialist users. For example, if performance is low and parameter x is unfavourable, the algorithm can gives an incentive to improve the parameter such as: "you COULD, SHOULD or MUST change parameter x". Regarding energy performance analysis, the following domains are addressed: heating, domestic hot water, cooling, lighting. Regarding the parameters, the analysis covers the following topics: Characteristics of the building envelope. and of the technical installations (heat production-distribution, ventilation system, electric lighting, etc.). This paper describes the methodology used, lists the fields studied and outlines the expected outcomes of the project.
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