Дисертації з теми "OsMADS4"
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Gregis, V. "Analisi comparata del controllo molecolare dell'induzione alla fioritura: ruolo dei geni OsMADS22/OsMADS47 e SVP/AGL24 rispettivamente in riso e Arabidopsis." Doctoral thesis, Università degli Studi di Milano, 2007. http://hdl.handle.net/2434/58412.
Повний текст джерелаKhong, Ngan Giang. "Etude fonctionnelle de facteurs de transcription OsMADS25 et OsMADS26 dans le développement et dans la réponse aux différents stress biotique et abiotique chez le riz." Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20120.
Повний текст джерелаMADS-box transcription factors (TF) have been mostly characterized for their involvement of plant development such as floral organogenesis and flowering time. Some of them are involved in stress related developmental processes such as abscission, fruit ripening and senescence. Overexpression of the rice OsMADS26 TF suggested a function in stress response. Here we report that OsMADS26 interfered lines presented a better resistance against two major pathogens of rice, Xanthomonas oryzae (Xoo) and Magnaportae oryzae (Mo) and a better recovery capacity after a water stress period. Transcriptome analysis revealed that several biotic and abiotic stresses related genes were up regulated in OsMADS26 interfered lines. In addition QPCR analysis showed that the expression of a set of biotic and abiotic genes was induced when OsMADS26 interfered lines were infected by Xoo or submitted to a water stress. This indicated that OsMADS26 is a negative regulator of biotic and abiotic stress response in rice. Taking in account the data previously published that showed that inducible overexpression of OsMADS26 resulted in the activation of expression of genes involved in jasmonic acid or reactive oxygen species biosynthesis, we postulate that OsMADS26 may be a hub regulator of stress response in rice and that it may be posttranscriptional regulated to modulate negatively or positively rice response to various stresses.In addition we have shown in this thesis that an insertion mutant line disrupting the OsMADS25 gene is characterized by a reduced number of tiller. This phenotype was also obtained in transgenic lines expressing the OsMADS25 transcription factor fused with a dominant motif inhibitor of transcription. Thissuggested that OsMADS25 is involved in the control of tiller development in rice.Key words: Rice, stress, blast, tillering, MADS-box, transcription factor, OsMADS26, OsMADS25, transcriptome
Zamzam, Mohamed. "Rice transcription factors OsMADS2 and OsMADS4 regulate floret organ development: Deciphering their gene targets, traits and functions related to their unequal genetic redundancy." Thesis, 2023. https://etd.iisc.ac.in/handle/2005/6185.
Повний текст джерелаLin, Shu-Yu, and 林書宇. "Studies on rice genes involved in early flowering regulated by OsMADS14 and panilce exsertion regulated by OsMADS34." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/77230047457895666724.
Повний текст джерела國立中興大學
分子生物學研究所
105
M52048, a T-DNA insertion mutant, shows dwarf, early flowering, node bending and impaired in panicle exsertion and it has been demonstrated to have three flanking genes OsMADS14, OsMADS34 and OsCP7 activated. Transgenic plants, Ubi:OsMADS14, ectopically overexpressing OsMADS14 revealed dwarf and early flowering phenotype, and up-regulated expression of florigen genes Hd3a and RFT1 were observed in the previous study. The first part of this study is to investigate the possible mechanisms that how the expression of Hd3a and RFT1 can be up-regulated by OsMADS14. At first, the expression profile of rice florigen regulators such as OsPRR37, OsCO3, DTH2, OsDof12, PPS and RFL were analyzed. Among them, the expression of RFL was promoted and OsCO3 was repressed suggesting that the activation of Hd3a and RFT1 might result from the up-regulation of RFL and/or down-regulation of OsCO3. Secondly, the phase transition miRNAs, miR156 and miR172 were investigated and results showed the expression of miR156 was slightly activated while miR172 was repressed suggesting that the delayed phase transition might occurred in Ubi:OsMADS14. Thirdly, the expression of potential floral repressors such as putative OsTOE1 (TARGET OF EAT1), putative OsTEM1 (OsTEMPRANILLO1), putative OsTEM2 and RCN1 that may regulate by OsMADS14 were analyzed. Results showed that OsTEM1 and RCN1 were activated, suggesting the possible involvement of partial counteraction of the effect of early flowering by OsMADS14. Finally, physiological functions of these putative floral repressors were studied by investigating their correspondent T-DNA insertion activation mutants such as M59289 (for OsTOE1), M78020 (for RCN1) and M89461 (for OsTEM1). Among these mutants, only the putative OsTEM1 gene in mutant M89461 was activated and a slightly late flowering phenotype was observed in mutant M89461 as well suggesting that the putative OsTEM1 might be a floral repressor in rice. Transgenic plants, Ubi:OsMADS34, ectopically overexpressing OsMADS34 revealed impaired panicle exsertion phenotype and that resulted by the reduced length of the first internode was previously demonstrated. The second part of this study is to investigate the possible mechanisms that how the length of the first internode was significant reduced by overexpressing OsMADS34. Results of the microarray assays comparing Ubi:OsMADS34 to TNG67 revealed differential expressed of many stress-related genes. Among them, 31 genes designated as M1 to M31 were selected for further study and their expressions were to be confirmed by RT-PCR. Results showed that the expression profile of M1, M2, M4, M8, M9, M10, M16, M17, M18, M21, M22, M23, M24, M26, M27, M30 and M31 were in accordance with the results of microarray assays suggesting that the expression of these genes were regulated by overexpression of OsMADS34.
Li, Tzu-Yin, and 李咨胤. "Characterization of the T-DNA insertion mutant M52048 and functional study of three activated genes OsMADS34、OsMADS14 and OsCP7." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/gp7a2s.
Повний текст джерела國立中興大學
分子生物學研究所
99
A rice T-DNA insertion mutant M0052048 showing extreme early flowering, bending tillers and impairment in panicle exertion was isolated from the Taiwan Rice Insertion Mutant (TRIM) library. This mutant contained a copy of the T-DNA tag inserted in the chromosome number 3 at the 64,867 bp position of OSJBa0032E21 BAC clone where the expression of 048-3 (OsMADS34), 048-4 (OsMADS14) and 048-7 (putative cysteine proteinase, OsCP7) genes were activated by the 35S enhancer located in the T-DNA. The OsMADS14 and OsMADS34 are MADS box-containing transcription factors that have important roles in plant growth and development and furthermore, OsCP7 is a putative cysteine proteinase that may be involved in the program cell death, pollen and xylem maturation, embryogenesis and flowering time in plants. In order to understand the functions of these activated genes and their contribution to the mutant phenotype, transgenic rice over-expressing each of these genes were created and expression profiles of these genes in various rice plant tissues were analyzed. RT-PCR analysis revealed that OsMADS14 and OsMADS34 expressed mainly in panicles but not necessarily in vegetative tissues, with the exception of a relatively high expression level of OsMADS14 in the 90-day-old leaf; For OsCP7, no clear expression signals were detected in all tested tissues. Transgenic rice Ubi:MADS14 showed constitutive expression of OsMADS14 in leaf tissue and revealed extreme early (63.6 days vs 121.3 days in TNG67) flowering and tiller bending phenotypes. The transgenic rice Ubi:MADS34 showed constitutive expression of OsMADS34 and revealed impairment in panicle exertion and slightly early (110 days vs 121.3 days) flowering compared to TNG67. For the overexpression study of OsCP7, no transgenic plant with ubiqutin promoter construct (Ubi:CP7) was obtained, thus a 1.6 kb promoter region from native OsCP7 gene was used to replace the ubiqutin promoter and several ectopically-expressed OsCP7 transgenic rice plants were obtained. These OsCP7 transgenic rice plants showed increased levels of OsCP7 mRNA and protein and higher cysteine protease activity compare to that of TNG67. In addition, these plants had reduced height, approximately 88% of TNG67, and revealed brown lesions on the surfaces of most spikelets that were neither observed in the TNG67 nor in M0052048. In summary, the present study suggests that the activation of OsMADS14 and OsMADS34 genes contributes to early flowering, bending tillers and impairment in panicle exertion phenotypes in the mutant M0052048. However, the effect of OsCP7 activation in mutant M0052048 and the function of OsCP7 in rice plants are still not clear and further investigations will be needed to answer these questions.
Yadav, Shri Ram. "Functions For OsMADS2 And OsMADS1 As Master Regulators Of Gene Expression During Rice Floret Meristem Specification And Organ Development." Thesis, 2009. https://etd.iisc.ac.in/handle/2005/2030.
Повний текст джерелаYadav, Shri Ram. "Functions For OsMADS2 And OsMADS1 As Master Regulators Of Gene Expression During Rice Floret Meristem Specification And Organ Development." Thesis, 2009. http://etd.iisc.ernet.in/handle/2005/2030.
Повний текст джерелаChen, Ying-Chin, and 陳潁芩. "Functional analysis of rice OsMADS14 and OsCP7 genes." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/69776772634934706215.
Повний текст джерела國立中興大學
分子生物學研究所
103
The T-DNA mutant M52048 identified from Taiwan Rice Insertional Mutant (TRIM) library showed dwarf, early flowering, node bending and impaired in panicle exertion. Three flanking genes, OsMADS34, OsMADS14 and OsCP7 (putative cysteine protease 7) were activated in this mutant. Both OsMADS34 and OsMADS14 belong to MADS-box gene family that may participate in regulation of flowering time and the identity of floral organ. OsCP7 is a member of C1A cysteine proteases. In this study, the function of OsMADS14 and OsCP7 were further investigated. Previous study has demonstrated that over-expression of OsMADS14 could cause early flowering. To understand whether or not any other flowering regulatory genes were affected by the expression of OsMADS14. The flowering regulatory genes including OsGI, OsMADS50, Ehd2, Hd1, Ehd1, Hd3a, RFT1, OsMADS14 and OsMADS18 were investigated in mutant M52048 and Ubi:OsMADS14, Ubi:OsMADS34 and OsCP7:OsCP7 transgenic rice respectively. Results showed two florigen genes, Hd3a and RFT1, expressed much earlier in M52048 and Ubi:OsMADS14 but not in Ubi:OsMADS34 and OsCP7:OsCP7, suggesting that Hd3a and RFT1 were regulated by the expression of OsMADS14. The mechanism how the expression of OsMADS14 could regulate florigen genes requires further investigation. Expression of OsCP7 driven by the maize ubiquitin promoter or the CaMV 35S promoter in transgenic rice cannot be obtained successfully. However transgenic rice, OsCP7:OsCP7 using 1.6 kb of OsCP7 promoter could be easily obtained and OsCP7:OsCP7 transgenic rice plants revealed slightly shorter in plant height, delayed flowering, lower fertility and lesion-like spots on spikelet. In contrast to the wild-type where no OsCP7 was detected in panicles, the RNA and protein expressions of OsCP7 in OsCP7:OsCP7 transgenic rice were detected in leaves at all development stages and panicles, and their expressions in transgenic rice correlated to the observed phenotypes. In addition, the phenotypes of segregated homozygous plants showed more significant than those of heterozygous plants within the same transgenic line, suggesting the dosage effect of transgene. However the expression levels of RNA and protein cannot be differentiated between homo- and hetero-zygous lines. To unravel the causes that lead to lower fertility of OsCP7:OsCP7, the floral organ and pollen viability were investigated. The floral organ showed no obvious differences between wild-type and OsCP7:OsCP7, but the pollen viability of OsCP7:OsCP7 was lower than that of wild-type, indicating that continuing expression of OsCP7 influence pollen development. Further investigation also indicated that the lesion-like spots on spikelet was correlated with the expression levels of OsCP7 and the lesions could possibly due to the programmed cell death caused by the activity of increased mature OsCP7 present in spikelet.
Chen, Chou-Fan, and 陳秋帆. "Functional analysis of rice OsMADS14 in transgenic plants." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/85953912950478779278.
Повний текст джерела輔英科技大學
生物科技系碩士班
100
Oncidium is one of the most important orchids used for cut flowers and potted plants in Taiwan. The creation of new cultivars with novel traits is important for Oncidium to enhance the competitiveness of orchid industry. However, traditional breeding processes are limited by the long life cycle and self-incompatibility. The objectives of this study intend to analyze rice AP1-like gene OsMADS14 in Arabidopsis and Oncidium through genetic transformation. In this study, the rice gene OsMADS14 driven by maize ubiqutin promoter was introduced into Arabidopsis and Oncidium Gower Ramsey mediated by Agrobacterium tumefaciens. Twenty one independent ubiquitin::OsMADS14 transgenic Arabidopsis plants were produced, confirmed by PCR and RT-PCR analysis. The ectopic expression of OsMADS14 in transgenic Arabidopsis plants showed dwarf, early flowering and terminal flowers. In addition, the OsMADS14 gene were introduced into Oncidium Gower Ramsey using Agrobacterium tumefaciens-mediated transformation, protocorm-like bodies ( PLBs ) of Oncidium were used as explants materials for genetic transformation and selected on medium containing 5ppm hygromycin. The resistant transgenic Oncidium were analyzed by PCR, RT-PCR and histochemical GUS assay, indicating the transgene integrated into the genome. The resistant transgenic Oncidium showed leave bending phenotype. The results of this study suggest that the rice OsMADS14 gene could be applied early-flowering and dwarf traits to ornamental flowers.
Chang, Fei-Han, and 張斐涵. "Overexpression of OsMADS34 and OsCP7 affecting panicle development in rice." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/18782266113590810452.
Повний текст джерела國立中興大學
分子生物學研究所
103
The T-DNA mutant M52048 identified from Taiwan Rice Insertional Mutant (TRIM) library showed dwarf, early flowering, node bending and impaired in panicle exertion. Three flanking genes, OsMADS34, OsMADS14 and OsCP7 (putative cysteine protease 7) were activated in this mutant. Both OsMADS34 and OsMADS14 belong to MADS-box gene family that may participate in regulation of flowering time and the identity of floral organ. OsCP7 encode a putative cysteine protease, belongs to C1A cysteine protease family, its function remains unknown. In this study the function of OsMADS34 and OsCP7 were further investigated. Previous study showed that over-expression of OsMADS34, Ubi:OsMADS34 transgenic rice, could cause slightly early flowering and impaired in panicle exertion. Morphological dissection indicated that the impaired in panicle exertion was mainly caused by shortening the first internode (peduncle). Inhibition of the peduncle elongation caused by drought stress and ABA accumulation has been reported. In the present study, some drought-related genes, such as DREB1A, DREB1E and EATB, were regulated in Ubi:OsMADS34 transgenic rice and therefore hypothesized that the phenotype of transgenic rice may regulated by drought stress or plant hormones. However, treated transgenic rice with ABA inhibitor and/or GA could not improve the peduncle elongation and panicle exertion, suggested that the shortened peduncle and impaired in panicle exertion in Ubi:OsMADS34 transgenic rice might not cause by ABA accumulation. Analysis of GA biosynthesis related genes and the cell elongation promoting genes, at the internodes, revealed high expression levels of EATB, EUI1, GA13ox1, GA20ox2, OsPK1 and lower expression of XTH28, suggested that the shortened internode might due to the imbalance expression of these genes. However the mechanism how these genes involved in internode elongation remain to be elucidated. In addition to the shortened peduncle, the anther development was also affected in Ubi:OsMADS34, suggesting that OsMADS34 function as an E class MADS-box gene may interact with other MADS-box genes to regulate the floral organ development. Further study by searching OsMADS34 interaction proteins will help us to unravel the possible function of OsMADS34. For the study of OsCP7 gene, we were unable to obtained stable transgenic rice lines with constitutive promoter constructs, suggesting that constitutively ectopic expression of OsCP7 might cause lethal. Instead, transgenic lines with a 1.6 kb of OsCP7 promoter construct, OsCP7:OsCP7, were successfully obtained. OsCP7:OsCP7 revealed slightly dwarf, delayed flowering, lesion-like spots on panicles and lower fertility, and these phenotypes are correlated to the expression of OsCP7 gene. The possible mechanisms that cause these aberrant panicle developments were under investigated.
Lin, Shih-Min, and 林仕敏. "Functional studies of the flowering regulative genes OsMADS14/15/18 in TNG67." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/sx9t54.
Повний текст джерела國立中興大學
分子生物學研究所
107
Flowering is an important developmental process for seed producing in plants. Previous studies had showed that respective over-expressing OsMADS14, OsMADS15 and OsMADS18 in rice would cause early flowering phenotype, implied that the three genes might play vital roles in regulating flowering process of rice. Moreover, the accumulation of OsMADS14 in both of T-DNA activation tagging mutant M52048 and Ubi:OsMADS14 transgenic rice result in dwarf and early flowering phenotype, which accompanied with activation of Hd3a, RFT1 and OsMADS15, raising the possibility that these genes might cooperate with OsMADS14 in regulating flowering. Nevertheless, the ability of the three genes to promote flowering have not investigated and compared in the same genetic background yet, to elucidate among the three genes, which gene is mainly involved in regulating flowering process, the transgenic approach and CRISPR/Cas9 knockout approach for OsMADS14, OsMADS15 and OsMADS18 were conducted in cultivar TNG67. In the present study, most of the Ubi:OsMADS15 transgenic rice displayed dwarf and early flowering phenotypes, however, instead of promoting flowering, the accumulation of OsMADS18 in Ubi:OsMADS18 result in delayed flowering and had no obvious effect on plant height growth, indicated that OsMADS18 might not have ability to promote flowering in rice. Besides, according to the expression comparison of Hd3a, RFT1 and OsMADS14, OsMADS15 and OsMADS18 among the Ubi:OsMADS15 transgenic rice, we found that early flowering phenotype was not observed from the transgenic rice which had no activated Hd3a, RFT1 and OsMADS14, suggested that overexpression of OsMADS15 along is not enough to promote flowering. On top of that, the flowering time of Ubi:OsMADS15 might promote by coexistence of Hd3a, RFT1, OsMADS14 and OsMADS15, and how of both Hd3a and RFT1 were activated in Ubi:OsMADS14 and Ubi:OsMADS15 transgenic rice still needed to be investigated in future. In addition, the CRIPSR/Cas9 genome editing system was applied for OsMADS14, OsMADS15 and OsMADS18 gene knockout in this study. In the flowering time comparison among these osmads14, osmads15 and osmads18 gene knockout mutants, our preliminary data showed that both of osmads14 and osmads15 had no obvious delay on flowering time. Surprisingly, the flowering time was promoted earlier in T1 progenies of osmads18, which corresponded to the result observed from Ubi:OsMADS18, implied that OsMADS18 might function as a negative regulator rather than positive regulator in regulating rice flowering. Since all of these data were observed and collected from either T0 or T1 progenies of osmads14, osmads15 and osmads18 knockout mutants, the functional inference of OsMADS14, OsMADS15 and OsMADS18 in regulating rice flowering still needed to be further confirmed in genetic stable T2 progenies. In addition, according to the flowering pathway in Arabidopsis, which had showed that TEM1 is a flowering repressor, therefore, we speculated that pOsTEM1 might also play as flowering repressor in rice. To investigate the function of TEM1 homologous gene pOsTEM1, the pOsTEM1 activation mutant M89461 was isolated from TRIM data base, besides, the pOsTEM1 overexpression transgenic rice was also created in current study. Surprisingly, our preliminary data showed that the accumulation of pOsTEM1 in both of T-DNA mutant M89461 and Ubi:pOsTEM1 transgenic rice result in late flowering phenotype, suggested that delayed flowering phenotype might result from accumulation of pOsTEM1. However, whether the pOsTEM1 is a flowering repressor in rice still needed to be further confirmed by further experiment in future.
Wang, Jiun-Da, and 王俊達. "Molecular regulation and agronomic traits of ectopic expression of OsMADS45 in transgenic rice." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/06426105691391245374.
Повний текст джерела國立中興大學
生命科學系所
101
The rice gene, OsMADS45, which belongs to the MADS-box E class gene, participates in the regulation of floral development. Previous studies have revealed that ectopic expression of OsMADS45 induces early flowering and reduces plant height under short-day (SD) conditions. However, the regulation mechanism of OsMADS45 overexpression remains unknown. We introduce an OsMADS45 overexpression construct Ubi:OsMADS45 into TNG67 plants (an Hd1 (Heading date 1) and Ehd1 (Early heading date 1) defective rice cultivar grown in Taiwan), and we analyzed the expression patterns of various floral regulators to understand the regulation pathways affected by OsMADS45 expression. The transgenic rice exhibit a heading date approximately 40 days earlier than that observed in TNG67 plants, and transgenic rice display small plant size and low grain yield. OsMADS45 overexpression did not alter the oscillating rhythm of the examined floral regulatory genes, Hd3a (Heading Date 3a) and RFT1 (RICE FLOWERING LOCUS T1), but advanced (by approximately 20 days) the up-regulation of the two florigens and suppressed the expression of Hd1 at the juvenile stage. The expression levels of OsMADS14 and OsMADS18, which are two well-known reproductive phase transition markers, were also increased at early developmental stages and are believed to be the major regulators responsible for early flowering in OsMADS45-overexpressing transgenic rice. OsMADS45 overexpression did not influence other floral regulator genes upstream of Hd1 and Ehd1, such as OsGI (OsGIGANTEA), Ehd2/Osld1/RID1 and OsMADS50. These results indicate that in transgenic rice, OsMADS45 overexpressing ectopically activates the upstream genes Hd3a and RFT1 at early developmental stage and up-regulates the expression of OsMADS14 and OsMADS18, which promote early flowering.
Khanday, Imtiyaz. "Target Genes and Pathways Regulated by OsMADSI during Rice Floret Specification and Development." Thesis, 2013. http://etd.iisc.ac.in/handle/2005/3395.
Повний текст джерелаKhanday, Imtiyaz. "Target Genes and Pathways Regulated by OsMADSI during Rice Floret Specification and Development." Thesis, 2013. http://etd.iisc.ernet.in/2005/3395.
Повний текст джерелаHuang, Jian-Fu, and 黃建富. "Rice gene functional analysis of panicle aberrant development in ectopically overexpressed OsMADS34 and OsCP7." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/24584460457880853165.
Повний текст джерела國立中興大學
分子生物學研究所
101
M52048 is a rice mutant with T-DNA insertion which activated flanking genes OsMADS14, OsMADS34 and OsCP7, resulting in darwf, early flowering, node bending and impaired panicle exertion. OsMADS14 and OsMADS34 belong to MADS-box gene family, can regulate the flowering time and the floral meristem development. OsMADS14 regulate the flowering time and has been well studies. OsMADS34 has been known to regulate the floral meristem development and panicle branching, OsCP7 encodes a putative cysteine protease, but the function by overexpressing OsMADS34 and OsCP7 genes remained unknown. The OsMADS34 overexpressing transgenic rice, Ubi:OsMADS34, revealed early flowering and impaired in panicle exertion. Morphological dissection indicated that the impaired panicle exertion was caused by shortening the first and second internodes. Previous study showed drought stress inhibited panicle exertion by reducing the peduncle elongation during flowering and induced the accumulation of ABA. The present study showed some dorught-related genes and miRNAs were regulated in Ubi:OsMADS34, and therefore, hypothesized that the phenotype of Ubi:OsMADS34 may regulated by drought stress or plant hormones. However, ABA inhibitor and GA treatments in this study could not improve the first internode elongation and panicle exertion. Gene expressinon analysis indicated the GA-regulated gene, EATB, was up-regulated and cell elongation promoting genes, XTH19 and XTH28, were reduced in the first and second internodes of Ubi:OsMADS34, suggesting that the limited internode elongation may be due to the differential expression of these internode regulating genes. However, how these genes were regulated remains further elucidation. OsCP7, like a typical cysteine protease, consist of a signal peptide, a prodomain and a protease domain, expressed in vegetative tissues but not in reproductive tissues. No OsCP7 overexpressing transgenic rice driven by ubiquitin or 35S promoters was stably obtained, though few of them could survive in the medium. Instead of these consititutive promoters, a 1.6 kb promoter fragment of OsCP7 could drive the expression of OsCP7 in rice successfully. This 1.6 kb OsCP7 promoter-driven transgenic rice, OsCP7:OsCP7, showed slightly dawrf, delayed flowering, low pollen viabillity, lower fertility seeds, and lesion-like brown spots appeared on spikelet. The lesion-like spots were first occuerd on the outer surface of spikelet when exposed to sunlight after heading. The spots could spread quickly in days and led to extensive cell death with ROS accumulation. However, using RNAi approach to knockdown the expression of OsCP7 did not show any specific phenotype. Further investigation to understand the molecular mechanism of the formation of the lesion-like spot is underway.
Lhaineikim, Grace. "Delineating the Role of OsMADS1 in Auxin Distribution, Floret Identity and Floret Meristem Determinacy." Thesis, 2016. http://etd.iisc.ac.in/handle/2005/2853.
Повний текст джерелаLhaineikim, Grace. "Delineating the Role of OsMADS1 in Auxin Distribution, Floret Identity and Floret Meristem Determinacy." Thesis, 2016. http://etd.iisc.ernet.in/handle/2005/2853.
Повний текст джерелаKartha, Reena V. "Regulated Expression Of OsMADS1, A MADS Domain Containing Transcription Factor, Involved In Rice Floret Development." Thesis, 2005. https://etd.iisc.ac.in/handle/2005/2198.
Повний текст джерелаKartha, Reena V. "Regulated Expression Of OsMADS1, A MADS Domain Containing Transcription Factor, Involved In Rice Floret Development." Thesis, 2005. http://etd.iisc.ernet.in/handle/2005/2198.
Повний текст джерелаParab, Aniket Girish. "Functional Characterization of a SAND–Domain–containing Factor OsULTRAPETALA1 – a Direct Downstream Target of OsMADS1." Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5458.
Повний текст джерелаFan, Hsiu-Tzu, and 范秀姿. "Establishment of transformation system for mini and early flowering gene in phalaenopsis Orchid- applications of GA2ox6 and OsMADS14." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/7yp5be.
Повний текст джерела國立中興大學
分子生物學研究所
102
The flowers of Phalaenopsis orchid have beautiful shape, brightly colors with long flowering period, are mostly used for commercial purposes, such as ornamental layout. Due to the semi-tropical location and climate condition, Taiwan becomes the most appropriate location for planting orchids and this makes orchids the most important export flowers in Taiwan, and consequently makes Taiwan as the "Orchid Kingdom" in the world. The diversities of orchid were mainly crossbred in the past; however many studies using transgenic or mutagenesis methods to change the color and/or appearance of the orchid morphology have been reported recently. Among them, few were used to change the plant architectures. In order to minimize and create novel orchid architectures, a rice OsGA2ox6 gene, involved in GA biosynthesis and when it over-expressed it could produce semi-dwarf plants with multiple shoots and roots, was used in this study. In addition to the long flowering period, the Phalaenopsis orchids have at least 2 to 3 year of growth period to reach the flowering stage, therefore more planting costs were needed. In order to shorten the time need before flowering, a MADS-box gene, MADS14, involved in the regulation of floral initiation and early flowering, was used under the control of a ubiquitin promoter as well. In this study, totally 6550 protocorm-like bodies (PLBs) were transformed by pCAM1301、 pCAM1301-Ubi-GA2ox6 and pCAM1301-Ubi-MADS14 vectors respectively. After hygromycin selection, three regenerated orchid plants from pCAM1301-Ubi-GA2ox6 were obtained and named as GA-1、GA-2 and GA-3, respectively. Comparing to the non-transgenic orchid, these regenerated orchids show dwarfism, multiple shoots and roots, and have smaller and dark-green leaf. All three regenerated orchids show GUS activity in examined leaf and root tissues. Genomic PCR analysis demonstrated that these three regenerated orchids contain the GUS and hptII genes and the GA2ox6 transgene as well. RNA expressions of the GA2ox6 transgene in these three regenerated orchids were detected by RT-PCR and showed the same expression levels among them. Southern blot assay showed these three regenerated orchids were independent transgenic orchid lines with one copy of T-DNA insertion in both GA-1 and GA-3, and two copies of T-DNA insertion in GA-2 line.
Prakash, Sandhan. "Investigating Partners of OsMADS1 Transcription Factor and functions for some associated factors for roles in rice inflorescence and floral development." Thesis, 2020. https://etd.iisc.ac.in/handle/2005/5024.
Повний текст джерелаIISc
Hsu, Min-Yu, and 許民育. "Studies on the molecular mechanisms involved in OsMADS14-regulated early flowering and evaluation on the establishment of an inducible transgenic platform in rice." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/80131340567549992713.
Повний текст джерела國立中興大學
生命科學系所
104
The early flowering phenotype of a rice T-DNA insertion mutant M52048 had been demonstrated due to the activation of OsMADS14, an Arabidopsis AP1 homologous gene involved in flowering regulation. Overexpressing OsMADS14 with ubiquitin promoter construct Ubi:OsMADS14 in TNG67 revealed early flowering as observed in M52048. In addition, the early expression of two floregin genes Hd3a and RFT1 were observed and enhanced in Ubi:OsMADS14 transgenic rice. However, how the activation and overexpression of OsMADS14 in rice could result in early flowering as AP1 did in the Arabidopsis have not been characterized. The present study aims to understand the molecular mechanisms of early flowering that regulated by over-expressing OsMADS14. The rice homologous to Arabidopsis AP1-regulated genes that lead to early flowering and dwarf, such as floral repressors, RCN 1 (homologous to TFL1), OsTEM1-like (TEM1), OsTOE1-like (TOE1); cytokinin biosynthesis genes, such as OsLOG, OsLOG1-like, OsLOG2-like, OsLOG3-like, OsCKX4, OsCKX5; and GA biosynthesis related genes, such as OsEUI1 and OsGA13ox1, were identified and investigated. Results showed Hd3a and RFT1 expressed much earlier in Ubi:OsMADS14 transgenic rice, and OsLOG and OsCKX5 response similarly to their homologous genes LOG1 and CKX3 in Arabidopsis, which may reduce axillary meristem growth and promote flowering. Surprisingly, the flowering suppressor gene RCN1 was enhanced, response differently from that in Arabidopsis. Therefore, the role of RCN1 in rice required further investigation. Regarding to the GA biosynthesis-related genes, the expression level of OsEUI1and OsGA13ox1 were enhaced that could possibly explain the dwarf phenotype of Ubi:OsMADS14 transgenic rice. The function of rice genes LOC_Os11g05470 (RCN1); LOC_Os05g03040 (OsTOE1-Like); LOC_Os01g49830 (OsTEM1-Like) homologous to Arabidopsis flowering suppressor genes AtTFL1, AtTOE1 and AtTEM1 were investigated. Two T-DNA insertion mutants, M78020 that may activate RCN1 and M89461 that may activate OsTEM1-like gene, were obtained from TRIM database for characterization. The insertion events of these two mutants were confirmed, and the mutant M89461 presente a late flowering phenotype, however mutant M78020 display no phenotype and no different in heading date to that of WT control. The links between the target genes expression and heading date phenotypes are under investigation. Establishing an alcohol inducible transgenic platform is another project of this study. This alcohol inducible system contains an alcohol-sensitive transcription factor AlcR driven by 35S promoter, and a second transcription unit contains AlcA promoter-driven target gene that can be regulated by alcohol-bound AlcR transcription factor. This alcohol inducible system using herbicide resistant bar gene as selection marker which is different from the hygromycin selection system we used routinely. Therefore, the optimization of selection condition was performed. Although several regenerated rice plant were obtained, none of them contain the transgene. In order to set up the alcohol inducible transgenic platform, replacing herbicide with hygromycin selection is suggested.