Дисертації з теми "Organotypic"
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Li, Daqing. "Entorhino-hippocampal projections in organotypic cultures." Thesis, University College London (University of London), 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315340.
Повний текст джерелаMbobo, Buchule. "Modelling neuroimmune interactions using organotypic slice cultures." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/24907.
Повний текст джерелаSteinmeyer, Joseph D. (Joseph Daly). "Rapid single-cell electroporation for labeling organotypic cultures." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/60188.
Повний текст джерелаVita.
Includes bibliographical references (p. 37-39).
Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in reasonable periods of time. A novel system that will rapidly load, clean, and accurately position a glass micropipette electrode into tissue culture for single-cell electroporation is proposed. The system will significantly increase the number of different labeling agents that can be introduced into a single tissue culture per unit time. This in turn, will provide a means for improving the study of neural anatomy at cellular resolutions in both tissue culture and in vivo environments.
Supported by grants from the National Institutes of Health and by the MIT Dept. of Electrical Engineering and Computer Science
by Joseph D. Steinmeyer.
S.M.
Vu, Lucas Trung. "Proteomic Analysis of Three Dimensional Organotypic Liver Models." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/77033.
Повний текст джерелаPh. D.
Kazmi, B. "Oral and dermal fibroblasts in 3D organotypic co-culture." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/17485/.
Повний текст джерелаFrantseva, Marina. "H¦2O¦2 induced toxicity in rat organotypic hippocampal cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0028/MQ51596.pdf.
Повний текст джерелаPhillips, Wiktor Samuel. "Studies of Respiratory Rhythm Generation Maintained in Organotypic Slice Cultures." W&M ScholarWorks, 2016. https://scholarworks.wm.edu/etd/1499449864.
Повний текст джерелаJussila, T. (Tommi). "Modelling cancer: recapitulation of tumor growth in experimental systems in vivo and in vitro." Doctoral thesis, University of Oulu, 2000. http://urn.fi/urn:isbn:9514256433.
Повний текст джерелаAcheva, Anna Rumenova. "Mechanisms of response to targeted irradiation in organotypic 3D skin cultures." Thesis, Queen's University Belfast, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.579565.
Повний текст джерелаFroeling, Fieke E. M. "Tumour–stroma interactions in an organotypic culture model of pancreatic cancer." Thesis, Queen Mary, University of London, 2011. http://qmro.qmul.ac.uk/xmlui/handle/123456789/705.
Повний текст джерелаFell, Benjamin. "Organotypic human skin disease models for the assessment of novel therapeutic approaches." Thesis, Queen Mary, University of London, 2017. http://qmro.qmul.ac.uk/xmlui/handle/123456789/24594.
Повний текст джерелаRioult-Pedotti, Marc Guy. "Optical multisite recording of neural activity patterns in organotypic spinal cord tissue cultures /." [S.l.] : [s.n.], 1991. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=9393.
Повний текст джерелаAjani, Gati. "Characterization of changes in hyaluronan following epidermal barrier injury in an organotypic model." Cleveland, Ohio : Cleveland State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1209996841.
Повний текст джерелаAbstract. Title from PDF t.p. (viewed on July 7, 2008). Includes bibliographical references (p. 150-176). Available online via the OhioLINK ETD Center. Also available in print.
El-Tarhouni, Amal Ibrahium. "Studies on the mechanosensory innervation of muscle using organotypic culture, reinnervation and immunohistochemistry." Thesis, Durham University, 1996. http://etheses.dur.ac.uk/5266/.
Повний текст джерелаGronbach, Leonie [Verfasser]. "Organotypic head and neck cancer models for advanced preclinical drug testing / Leonie Gronbach." Berlin : Freie Universität Berlin, 2021. http://d-nb.info/1230407316/34.
Повний текст джерелаMedelin, Manuela. "Degenerative processes in organotypic spinal slices: challenging pre-motor network with stress conditions." Doctoral thesis, Università degli studi di Trieste, 2014. http://hdl.handle.net/10077/10130.
Повний текст джерелаMy PhD project concerns neurodegenerative processes in the mouse spinal cord, with a particular attention to amyotrophic lateral sclerosis (ALS). During my PhD I have used as a model the organotypic spinal slice cultures and I have focused my studies on: early changes in spinal tissue excitability in an ALS genetic model; spinal network activity changes induced by oxidative stress in wild type (WT) and synaptic activity in premotor circuits when challenged by neuroinflammation in WT. The principal aim of my work was to understand the dialogue between a general stress condition and the spinal premotor network. To this aim, I combined electrophysiological techniques and immunofluorescence analysis to characterized the ventral interneurones located in spinal microcircuits. For that purpose I exploited the organotypic cultures developed from embryonic mouse spinal cord that are generally accepted as a good model to study the neuronal premotor activity and provide high experimental access to interneurones (Avossa et al., 2003). In the first part of my research I compared WT cultures with SOD1G93A transgenic cultures, one of the more investigated ALS model. In cultured spinal networks, as described in acute preparation collected at different stages of development, there is a progressive fastening of glycinergic currents, represented by the reduction of the decay time constant (tau value) of synaptic currents along with the slices growth. WT and SOD1G93A cultures display a different maturation profile since in transgenic slices this developmental process is significantly steeper not only in glycinergic post synaptic currents (PSCs) but also in miniature PSCs (mPSCs). This difference in the glycinergic PSCs kinetic properties can be strongly reduced by the presence of TBOA that lowers the GABA synthesis. These results support the hypothesis that in SOD1G93A cultures there is an increase amount of glycine and GABA co-release leading to the conclusion that the synaptic release is conditioned by the presence of the mutation at an early stage of development, before any evident neuronal degeneration. Moreover, I supported this data also with preliminary results regarding the co-staining of GlyT2 and GAD65 (markers for presynaptic glycine and GABA, respectively). In fact, SOD1G93A spinal organotypic slices seem to display an higher amount of mixed synapses. Next, I tested other stress processes of the tissue that could potentially affect synaptic activity and, ultimately, alter network activity. I tested chronic incubations of the spinal slices, since they are long-term preparations, with stress key players: hydrogen peroxide (H2O2) to create an oxidative stress and lipopolysaccharide (LPS) or a mixture of cytokines (CKs: TNF-α, IL-1β and GM-CSF) to mimic neuroinflammation. For these sets of experiments I have used another strain of mice with no genetic manipulation. All these chronic treatments increase the AMPA receptor mediated PSCs frequency; moreover, a neuroinflammation state is able to enhance the overall network activity; LPS treatment increases also the amplitude of AMPA-mediated synaptic currents in both spontaneous and miniature events, while CKs accelerate the disinhibited burst rhythm induced by the pharmacological removal of the synaptic inhibition that switch on the rhythmogenic centre contained in the spinal network. Summarizing, I detected that the treatments with these environmental cues affected the synaptic component, in this case the excitatory one, of the premotor network. Altogether, my work highlighted that a genetic predisposition (in the case of familial ALS) and environmental factors of different kind (oxidative and inflammatory factors or an alterate SOD1) trigger changes in synaptic transmission and we may speculate that these alterations in the premotor circuit could cooperate in synergy leading to the development of misconnected networks that contribute to induce motoneuronal neurodegeneration. Riassunto Il mio progetto di dottorato riguarda processi neurodegenerativi del midollo spinale, con una particolare attenzione verso la sclerosi laterale amiotrofica (SLA). Durante il mio dottorato ho usato come modello le fettine organotipiche di midollo spinale e ho concentrato i miei studi su: cambiamenti precoci nell’eccitabilità del tessuto spinale in un modello genetico di SLA; cambiamenti nell’attività del network spinale indotti da stress ossidativo in wild type (WT) e cambiamenti nell’attività sinaptica dei circuiti premotori sottoposti ad uno stress infiammatorio in WT. Il fine principale del mio lavoro era quello di capire il dialogo tra una condizione di stress generale ad il network spinale premotorio. A questo scopo, ho unito tecniche elettrofisiologiche e analisi di immunofluorescenza per caratterizzare gli interneuroni ventrali localizzati nel microcircuito spinale. Per raggiungere questo obiettivo ho sfruttato le colture organotipiche derivate dal midollo spinale di embrioni di topo che sono generalmente accettate come un buon modello per studiare l’attività premotoria neuronale e garantiscono un facile accesso sperimentale agli interneuroni (Avossa et al., 2003). Nella prima parte della mia ricerca ho confrontato colture WT con colture transgeniche SOD1G93A, uno dei modelli di SLA maggiormente studiati. Nei network spinali in coltura, come già descritto in preparazioni acute ottenute a diversi stadi di sviluppo, c’è una progressiva velocizzazione delle correnti glicinergiche, rappresentata dalla riduzione del decay time constant (valore di tau) delle correnti sinaptiche durante la crescita delle fettine. Le colture WT e SOD1G93A presentano un diverso profilo di maturazione dato che nelle colture transgeniche questo processo di sviluppo è significativamente più marcato non solo nelle correnti postsinaptiche (PSCs) ma anche negli eventi in miniatura (mPSCs). Questa differenza nelle proprietà cinetiche delle correnti glicinergiche può essere fortemente ridotta dalla presenza di TBOA che diminuisce la sintesi del GABA. Questi risultati supportano l’ipotesi che nelle colture SOD1G93A ci sia un aumento del co-rilascio GABA/glicina portando alla conclusione che il rilascio sinaptico sia condizionato dalla presenza della mutazione ad uno stadio precoce dello sviluppo, prima di qualsiasi degenerazione neuronale evidente. Inoltre, ho supportato questo dato anche con risultati preliminari riguardanti la marcatura di GlyT2 e GAD65 (due marker per la glicina ed il GABA presinaptici rispettivamente). Infatti, le fettine spinali organotipiche SOD1G93A sembrano caratterizzate da un aumento delle sinapsi miste. Successivamente, ho testato altri processi di stress del tessuto che potrebbero interferire con l’attività sinaptica e, conseguentemente, alterare l’attività del network. Dato che le fettine spinali sono preparazioni a lungo termine, ho testato incubazioni croniche con molecole chiave nei processi di stress: perossido di idrogeno (H2O2) per creare uno stress ossidativo e lipopolisaccaride (LPS) o una miscela di citochine (CKs: TNF-α, IL-1β and GM-CSF) per mimare uno stato infiammatorio. Per questo set di esperimenti ho usato un altro ceppo di topi privo di manipolazione genetica. Tutti questi trattamenti cronici aumentano la frequenza delle correnti mediate dai recettori AMPA; inoltre, uno stato infiammatorio è in grado di incrementare l’attività globale del network; il trattamento con LPS aumenta anche l’ampiezza delle correnti sinaptiche AMPA-mediate, sia spontanee che in miniatura, mentre le CKs accelerano il ritmo dei burst indotto dall’eliminazione farmacologica dell’inibizione sinaptica che accende il centro ritmogenico presente nel network spinale. Riassumendo, ho dimostrato che i trattamenti con questi fattori ambientali alterano la componente sinaptica, in questo caso eccitatoria, del network premotorio. Nel complesso il mio lavoro ha evidenziato che una predisposizione genetica (nel caso della SLA familiare) e fattori ambientali di varia natura (ossidativi, infiammatori o di alterata SOD1) inducono cambiamenti nella trasmissione sinaptica e possiamo speculare sul fatto che queste alterazioni nel circuito premotorio possono cooperare in sinergia causando lo sviluppo di network inefficienti che concorrono a determinare la neurodegenerazione motoneuronale.
XXVI Ciclo
1985
Patel, Leena Suman. "Functional changes in pyramidal neurons surviving an excitotoxic challenge in mouse organotypic slice cultures /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/10658.
Повний текст джерелаIsaacson, Erin. "Understanding the molecular basis of HPV-16 neoplastic progression using an organotypic raft model." Thesis, Open University, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522239.
Повний текст джерелаMeng, Weina. "Evaluation of a nanoparticle drug delivery vehicle in medulloblastoma and organotypic brain cell cultures." Thesis, University of Nottingham, 2006. http://eprints.nottingham.ac.uk/13933/.
Повний текст джерелаOrbach, Sophia Michelle. "Multi-Cellular Organotypic Liver Models for the Investigation of Chemical Toxicity and Liver Fibrosis." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/93313.
Повний текст джерелаPh. D.
Croft, Cara Louise. "Investigating the mechanisms underlying Alzheimer's disease using a novel organotypic brain slice culture model." Thesis, King's College London (University of London), 2016. http://kclpure.kcl.ac.uk/portal/en/theses/investigating-the-mechanisms-underlying-alzheimers-disease-using-a-novel-organotypic-brain-slice-culture-model(321a7046-c8e8-4c42-9abf-01792be7870c).html.
Повний текст джерелаStuder, Lorenz. "NGF increases neuritic complexity of cholinergic interneurons in organotypic cultures of neonatal rat striatum /." [S.l.] : [s.n.], 1994. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Повний текст джерелаCocchi, M. A. "THE MELATONIN PROTECTIVE ROLE IN AN ORGANOTYPIC MODEL OF SPINAL CORD INJURY SECONDARY DAMAGE." Doctoral thesis, Università degli Studi di Milano, 2016. http://hdl.handle.net/2434/351674.
Повний текст джерелаIreland, Kirsty Anne. "Development of whole brain organotypic slice culture to investigate in vitro seeding of amyloid plaques." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28707.
Повний текст джерелаBonilla-Pons, Sergi A. "Setting up stem cell therapy for human retina: from organotypic cultures to cell fusion understanding." Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/673448.
Повний текст джерелаSingh, Sunil. "Engineered Organotypic Breast Tumor Model for Mechanistic Studies of Tumor-Stromal Interactions and Drug Discovery." University of Akron / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1616940345310981.
Повний текст джерелаCAMBINI, AIMONE. "Effetti della biostimolazione laser sul tessuto epiteliale per il differenziamento dello strato cheratinizzato: studio in vitro." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2013. http://hdl.handle.net/10281/43489.
Повний текст джерелаVautrin, Sandrine. "Investigating oligodendrocyte development and stability using organotypic hippocampal slices, confocal imaging and viral gene delivery methods." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111543.
Повний текст джерелаSmith, Donna Louise. "An organotypic slice culture assay to assess huntingtin aggregation in Huntington's disease R6/2 transgenic mice." Thesis, King's College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406704.
Повний текст джерелаAthar, Saira. "Immortalization of human oral keratinocytes with defined genetic elements in the development of organotypic oral culture." Thesis, Queen Mary, University of London, 2013. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8690.
Повний текст джерелаDallacasagrande, Valentina. "Novel approaches to study the biomechanics of intact central nervous tissue." Doctoral thesis, Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-163943.
Повний текст джерелаMcBain, Christopher J. "An electrophysiological investigation of the spontaneously arising epileptiform activity seen in organotypic cultures of neonate rat hippocampus." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293815.
Повний текст джерелаStubblefield, Park Samantha Renee. "Organotypic brain explants reveal an interleukin-12 / interferon-γ / T-cell dependent clearance of measles virus infection". Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1297280439.
Повний текст джерелаSullivan, Aideen Margaret. "The use of organotypic cultures of rat cerebellum for the study of neuromodulatory interactions in the mammalian brain." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243197.
Повний текст джерелаJagger, Elizabeth. "The effects of kainic acid in hippocampal organotypic cultures : a chronic in vitro model of temporal lobe epilepsy." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269697.
Повний текст джерелаBarnhart, Kirstin Faye. "In vitro and in vivo analysis of differential gene expression between normal norfolk terrier dogs and those with an autosomal recessive mutation in KRT10." Texas A&M University, 2004. http://hdl.handle.net/1969.1/2671.
Повний текст джерелаWills, Lauren Raquel. "Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications." Thesis, Virginia Tech, 2019. http://hdl.handle.net/10919/101006.
Повний текст джерелаMaster of Science
Werner, Renata Verfasser], та Mathias [Akademischer Betreuer] [Jucker. "Modeling the prion aspect of cerebral β-amyloidosis in organotypic slice cultures and mice / Renata Werner ; Betreuer: Mathias Jucker". Tübingen : Universitätsbibliothek Tübingen, 2018. http://d-nb.info/1168728371/34.
Повний текст джерелаJevtić, Marijana [Verfasser]. "Fine-Tuning of Organotypic Skin Equivalents for Preclinical Research and Their Utilization to Study Epidermal-Dermal Crosstalk / Marijana Jevtić." Berlin : Freie Universität Berlin, 2021. http://d-nb.info/1229436650/34.
Повний текст джерелаNash, Claire. "Development of an organotypic 3D in vitro model of normal human breast tissue : a tool for cancer initiation studies." Thesis, University of Leeds, 2014. http://etheses.whiterose.ac.uk/6845/.
Повний текст джерелаMüller, Mareike [Verfasser], Horst-Werner [Akademischer Betreuer] Korf, Horst [Akademischer Betreuer] Stöcker, and Marco [Akademischer Betreuer] Durante. "Effects of ionizing radiation on organotypic slice cultures / Mareike Müller. Gutachter: Horst Stöcker ; Marco Durante. Betreuer: Horst-Werner Korf." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2013. http://d-nb.info/104397816X/34.
Повний текст джерелаOlivieri, Dario. "New Spinal cord models: Characterization of Excitotoxicity and Neuroprotection." Doctoral thesis, SISSA, 2015. http://hdl.handle.net/20.500.11767/3892.
Повний текст джерелаSygnecka, Katja. "Organotypic brain slice co-cultures of the dopaminergic system - A model for the identification of neuroregenerative substances and cell populations." Doctoral thesis, Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-188897.
Повний текст джерелаBarth, Melanie [Verfasser]. "Organotypic slice culture models for induced alpha-synucleinopathy and exploration of the potential role of microglia in pathogenesis / Melanie Barth." Tübingen : Universitätsbibliothek Tübingen, 2023. http://d-nb.info/1238595006/34.
Повний текст джерелаAldarwesh, Amal. "Oxygen and glucose deprivation on human Müller cells (MIO-M1) and human organotypic retinal cultures (HORCs) in relation to glaucoma." Thesis, University of East Anglia, 2015. https://ueaeprints.uea.ac.uk/58555/.
Повний текст джерелаLinnemann, Jelena [Verfasser], and Magdalena [Akademischer Betreuer] Götz. "An organotypic assay for the quantification and characterization of regenerative primary human mammary epithelial cells / Jelena Linnemann ; Betreuer: Magdalena Götz." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1154683699/34.
Повний текст джерелаNiel, Olivier. "Développement vasculaire rénal in vivo et ex vivo : vers la bio-ingénierie rénale." Thesis, Nice, 2014. http://www.theses.fr/2014NICE4023.
Повний текст джерелаIn mice, nephrogenesis starts with the formation of the metanephric mesenchyme, at e9.5 dpc. A mesenchymal epithelial transition, consisting of 5 steps, starts at e11.5 dpc, and leads to a mature kidney, composed of 3 main structures: glomeruli, tubules, and capillaries. The initial steps of renal development can be recapitulated ex vivo; however, terminal organogenesis and maturation are impaired, and the explants are not functional. A study of vascular development in vivo during renal development shows that angiogenesis (Pecam-1 positive cells) and vasculogenesis (VEGF-R1 positive cells) occur early, at e10.5 dpc. A quantitative analysis, by qRT-PCR, shows that Hif1α and VEGF play a major role in renal vasculogenesis. Moreover, the PGC1α signaling pathway, a HIF independent VEGF inductor, is activated under hypoxic conditions. To improve ex vivo vascular development, we propose a novel culture technique, with micro-perfusion of the explant. A morphologic analysis of the kidneys obtained by micro-perfused cultures shows no apoptosis (TUNEL), a conserved parenchymal structure (Nephrin, Cytokeratin, WT1), and a proper vascular development (Pecam-1). A micro-perfusion of WT1 vivo-morpholinos leads to a decrease in WT1 expression, thus validating our model. In conclusion, we showed the early role of angiogenesis and vasculogenesis in renal development, we analyzed PGC1α role in hypoxic kidney cultures, and we proposed a novel kidney culture model
Wang-Lauenstein, Lan [Verfasser]. "Assessment of chemical-induced local irritation and inflammation in organotypic lung tissue model : precision-cut lung slices (PCLS) / Lan Wang-Lauenstein." Hannover : Technische Informationsbibliothek (TIB), 2017. http://d-nb.info/1128673576/34.
Повний текст джерелаVerheyen, Leonie Katharina [Verfasser]. "Development of Organotypic Skin Disease Equivalents Mimicking Hallmarks of Atopic Dermatitis for Basic Research and Preclinical Drug Evaluation / Leonie Katharina Verheyen." Berlin : Freie Universität Berlin, 2018. http://d-nb.info/1176641182/34.
Повний текст джерелаSchmohl, Michael [Verfasser], and Stefan [Akademischer Betreuer] Stevanovic. "Analyzing biological activity of drugs- Inter- and intracellular signaling analysis within an organotypic co-culture system / Michael Schmohl ; Betreuer: Stefan Stevanovic." Tübingen : Universitätsbibliothek Tübingen, 2011. http://d-nb.info/1162699302/34.
Повний текст джерела