Готові списки джерел за темами / Orf121

Добірка наукової літератури з теми "Orf121"

Автор: Grafiati

Опубліковано: 7 липня 2024

Оновлено: 7 липня 2024

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Статті в журналах з теми "Orf121":

Rziha, Hanns-Joachim, Mathias Büttner, Melanie Müller, Ferdinand Salomon, Alena Reguzova, Dominic Laible, and Ralf Amann. "Genomic Characterization of Orf Virus Strain D1701-V (Parapoxvirus) and Development of Novel Sites for Multiple Transgene Expression." Viruses 11, no. 2 (January 30, 2019): 127. http://dx.doi.org/10.3390/v11020127.

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The Orf virus (ORFV; Parapoxvirus) strain D1701 with an attenuated phenotype and excellent immunogenic capacity is successfully used for the generation of recombinant vaccines against different viral infections. Adaption for growth in Vero cells was accompanied by additional major genomic changes resulting in ORFV strain variant D1701-V. In this study, restriction enzyme mapping, blot hybridization and DNA sequencing of the deleted region s (A, AT and D) in comparison to the predecessor strain D1701-B revealed the loss of 7 open reading frames (ORF008, ORF101, ORF102, ORF114, ORF115, ORF116, ORF117). The suitability of deletion site D for expression of foreign genes is demonstrated using novel synthetic early promoter eP1 and eP2. Comparison of promoter strength showed that the original vegf-e promoter Pv as well as promoter eP2 display an up to 11-fold stronger expression than promoter eP1, irrespective of the insertion site. Successful integration and expression of the fluorescent marker genes is demonstrated by gene- and insertion-site specific PCR assays, fluorescence microscopy and flow cytometry. For the first time ORFV recombinants are generated simultaneously expressing transgenes in two different insertion loci. That allows production of polyvalent vaccines containing several antigens against one or different pathogens in a single vectored ORFV vaccine.

Tang, Ruizhe, Liqun Lu, Beiyang Wang, Jiao Yu, and Hao Wang. "Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2." Viruses 12, no. 9 (September 7, 2020): 994. http://dx.doi.org/10.3390/v12090994.

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Cyprinid herpesvirus 2 (CyHV-2), which infects goldfish and crucian carp causing high mortality, is an emerging viral pathogen worldwide. The genome of CyHV-2 is large and comprises double-stranded DNA, including several genes similar to cyprinid herpesvirus 1, ictalurid herpesvirus-1, cyprinid herpesvirus 3, and ranid herpesvirus-1. Genes of DNA viruses are expressed in three temporal phases: immediate-early (IE), early (E), and late (L) genes. Viral IE genes initiate transcription as soon as the virus enters the host, without viral DNA replication. IE gene products enable the efficient expression of E and L genes or regulate the host to initiate virus replication. In the present study, five IE genes of CyHV-2 were identified, including open reading frame (ORF)54, ORF121, ORF141, ORF147, and ORF155. Time course analysis and reverse transcription polymerase chain reaction confirmed five IE genes, thirty-four E genes, and thirty-nine L genes. In addition, all 150 ORFs identified in the CyHV-2 genome are transcribed, and are expressed in chronological order, similar to other herpesviruses. This study is the first to identify the IE genes of CyHV-2, which will provide more information for viral molecular characterization.

Gong, Min, Jianping Jin, and Linda A. Guarino. "Mapping of ORF121, a Factor That Activates Baculovirus Early Gene Expression." Virology 244, no. 2 (May 1998): 495–503. http://dx.doi.org/10.1006/viro.1998.9116.

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Che, Xibing, Mike Reichelt, Marvin H. Sommer, Jaya Rajamani, Leigh Zerboni, and Ann M. Arvin. "Functions of the ORF9-to-ORF12 Gene Cluster in Varicella-Zoster Virus Replication and in the Pathogenesis of Skin Infection." Journal of Virology 82, no. 12 (April 9, 2008): 5825–34. http://dx.doi.org/10.1128/jvi.00303-08.

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ABSTRACT The gene cluster composed of varicella-zoster virus (VZV) open reading frame 9 (ORF9) to ORF12 encodes four putative tegument proteins and is highly conserved in most alphaherpesviruses. In these experiments, the genes within this cluster were deleted from the VZV parent Oka (POKA) individually or in combination, and the consequences for VZV replication were evaluated with cultured cells in vitro and with human skin xenografts in SCID mice in vivo. As has been reported for ORF10, ORF11 and ORF12 were dispensable for VZV replication in melanoma and human embryonic fibroblast cells. In contrast, deletion of ORF9 was incompatible with the recovery of infectious virus. ORF9 localized to the virion tegument and formed complexes with glycoprotein E, which is an essential protein, in VZV-infected cells. Recombinants lacking ORF10 and ORF11 (POKAΔ10/11), ORF11 and ORF12 (POKAΔ11/12), or ORF10, ORF11 and ORF12 (POKAΔ10/11/12) were viable in cultured cells. Their growth kinetics did not differ from those of POKA, and nucleocapsid formation and virion assembly were not disrupted. In addition, these deletion mutants showed no differences compared to POKA in infectivity levels for primary human tonsil T cells. Deletion of ORF12 had no effect on skin infection, whereas replication of POKAΔ11, POKAΔ10/11, and POKAΔ11/12 was severely reduced, and no virus was recovered from skin xenografts inoculated with POKAΔ10/11/12. These results indicate that with the exception of ORF9, the individual genes within the ORF9-to-ORF12 gene cluster are dispensable and can be deleted simultaneously without any apparent effect on VZV replication in vitro but that the ORF10-to-ORF12 cluster is essential for VZV virulence in skin in vivo.

Kessler, Peter S., Carrine Blank, and John A. Leigh. "The nif Gene Operon of the Methanogenic Archaeon Methanococcus maripaludis." Journal of Bacteriology 180, no. 6 (March 15, 1998): 1504–11. http://dx.doi.org/10.1128/jb.180.6.1504-1511.1998.

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ABSTRACT Nitrogen fixation occurs in two domains, Archaea andBacteria. We have characterized a nif(nitrogen fixation) gene cluster in the methanogenic archaeonMethanococcus maripaludis. Sequence analysis revealed eight genes, six with sequence similarity to known nif genes and two with sequence similarity to glnB. The gene order,nifH, ORF105 (similar to glnB),ORF121 (similar to glnB), nifD,nifK, nifE, nifN, andnifX, was the same as that found in part in other diazotrophic methanogens and except for the presence of theglnB-like genes, also resembled the order found in many members of the Bacteria. Using transposon insertion mutagenesis, we determined that an 8-kb region required for nitrogen fixation corresponded to the nif gene cluster. Northern analysis revealed the presence of either a single 7.6-kbnif mRNA transcript or 10 smaller mRNA species containing portions of the large transcript. Polar effects of transposon insertions demonstrated that all of these mRNAs arose from a single promoter region, where transcription initiated 80 bp 5′ tonifH. Distinctive features of the nif gene cluster include the presence of the six primary nif genes in a single operon, the placement of the two glnB-like genes within the cluster, the apparent physical separation of the cluster from any other nif genes that might be in the genome, the fragmentation pattern of the mRNA, and the regulation of expression by a repression mechanism described previously. Our study and others with methanogenic archaea reporting multiple mRNAs arising from gene clusters with only a single putative promoter sequence suggest that mRNA processing following transcription may be a common occurrence in methanogens.

Gao, Wa, Lupin Zhao, Yihua Zheng, Kaixuan Wu, Feiyang Xu, Hao Wang, Liqun Lu, and Yousheng Jiang. "Generation and application of a monoclonal antibody specific for the ORF121 of cyprinid herpesvirus 2." Journal of Fish Diseases 45, no. 3 (December 6, 2021): 387–94. http://dx.doi.org/10.1111/jfd.13566.

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Kolosov, A. V., V. A. Ternovoy, A. N. Shvalov, A. A. Moiseeva, A. S. Safatov, and V. N. Mikheev. "ADAPTATION OF THE CORN EARWORM SINGLE NUCLEOCAPCIDE NUCLEOPOLYHEDROVIRUS (HELICOVERPA ZEA SNPV) FOR THE CONTROL OF THE COTTON BOLLWORM (HELICOVERPA ARMIGERA) POPULATION." Problems of Virology, Russian journal 62, no. 3 (June 20, 2017): 134–37. http://dx.doi.org/10.18821/0507-4088-2017-62-3-134-137.

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Helicoverpa zea (Boddie, 1850) (Hz) single nucleocapcide nucleopolyhedrovirus (SNPV) was adapted to the cotton bollworm (Helicoverpa armigera, (Hübner, 1805) (Ha)) by five blind passages on larvae. The full genomic sequence of the resulting strain HS-18 has been determined (GenBank acc. №: KJ004000.1). Biological activity of the HS-18 strain is higher than the activity of all other Russian strains of NPV, as far as cotton bollworm strain HearSNPV-G4. HS-18-infected caterpillars at the 3-rd and 4-th ages died much faster than those infected with HearSNPV-G4 strain. A major difference of HS-18 genome is an 18 bp repeat in the RING-finger ORF that confirms high variability of this region. Three other insertions and seven base substitutions were observed earlier, while six base substitutions are new. Mutations are located at ORF42, lef-9, ORF58, VP39, PIF-4, P48, SOD, ORF111, ORF129 and ORF138 genes. Among all nucleotide mutation only one is synonymous. Thus we suppose the selective pressure to the virus. The resulting strain HS-18 is recommended as a biopesticide for controlling the number of cotton bollworm in cotton fields.

Martínez-Costa, Oscar H., Angel J. Martín-Triana, Eduardo Martínez, Miguel A. Fernández-Moreno, and Francisco Malpartida. "An Additional Regulatory Gene for Actinorhodin Production in Streptomyces lividans Involves a LysR-Type Transcriptional Regulator." Journal of Bacteriology 181, no. 14 (1999): 4353–64. http://dx.doi.org/10.1128/jb.181.14.4353-4364.1999.

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The sequence of a 4.8-kbp DNA fragment adjacent to the right-hand end of the actinorhodin biosynthetic (act) cluster downstream of actVB-orf6 from Streptomyces coelicolor A3(2) reveals six complete open reading frames, namedorf7 to orf12. The deduced amino acid sequences from orf7, orf10, and orf11 show significant similarities with the following products in the databases: a putative protein from the S. coelicolor SCP3 plasmid, LysR-type transcriptional regulators, and proteins belonging to the family of short-chain dehydrogenases/reductases, respectively. The deduced product of orf8 reveals low similarities with several methyltransferases from different sources, whileorf9 and orf12 products show no similarities with other known proteins. Disruptions of orf10 andorf11 genes in S. coelicolor appear to have no significant effect on the production of actinorhodin. Nevertheless, disruption or deletion of orf10 in Streptomyces lividans causes actinorhodin overproduction. The introduction of extra copies of orf10 and orf11 genes in anS. coelicolor actIII mutant restores the ability to produce actinorhodin. Transcriptional analysis and DNA footprinting indicate that Orf10 represses its own transcription and regulatesorf11 transcription, expression of which might require the presence of an unknown inducer. No DNA target for Orf10 protein was found within the act cluster.

Tan, Yeping, Dennis K. Bideshi, Jeffrey J. Johnson, Yves Bigot, and Brian A. Federici. "Proteomic analysis of the Spodoptera frugiperda ascovirus 1a virion reveals 21 proteins." Journal of General Virology 90, no. 2 (February 1, 2009): 359–65. http://dx.doi.org/10.1099/vir.0.005934-0.

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The Spodoptera frugiperda ascovirus 1a (SfAV-1a) is a double-stranded DNA virus that attacks lepidopteran larvae, in which it produces enveloped virions with complex symmetry which have an average diameter of 130 nm and length of 400 nm. Here, we report identification of 21 SfAV-1a-encoded proteins that occur in the virion, as determined by nano-liquid chromatography/tandem mass spectrometry. These included a helicase (ORF009), nuclease (ORF075), ATPase (ORF047), serine/threonine-like protein kinase (ORF064), inhibitor of apoptosis-like protein (ORF015), thiol oxidoreductase-like protein (ORF061), CTD phosphatase (ORF109), major capsid protein (ORF041) and a highly basic protein, P64 (ORF048). The latter two were the most abundant. Apart from ascoviruses, the closest orthologues were found in iridoviruses, providing further evidence that ascoviruses evolved from invertebrate iridoviruses. These results establish a foundation for investigating how ascovirus virion proteins interact to form their complex asymmetrical structure, as well as for elucidating the mechanisms involved in SfAV-1a virion morphogenesis.

Li, Ping, Shanghai Yong, Xin Zhou, and Jiayin Shen. "Characterization of a New Temperate Escherichia coli Phage vB_EcoP_ZX5 and Its Regulatory Protein." Pathogens 11, no. 12 (November 30, 2022): 1445. http://dx.doi.org/10.3390/pathogens11121445.

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The study of the interaction between temperate phages and bacteria is vital to understand their role in the development of human diseases. In this study, a novel temperate Escherichia coli phage, vB_EcoP_ZX5, with a genome size of 39,565 bp, was isolated from human fecal samples. It has a short tail and belongs to the genus Uetakevirus and the family Podoviridae. Phage vB_EcoP_ZX5 encodes three lysogeny-related proteins (ORF12, ORF21, and ORF4) and can be integrated into the 3′-end of guaA of its host E. coli YO1 for stable transmission to offspring bacteria. Phage vB_EcoP_ZX5 in lysogenized E. coli YO1+ was induced spontaneously, with a free phage titer of 107 PFU/mL. The integration of vB_EcoP_ZX5 had no significant effect on growth, biofilm, environmental stress response, antibiotic sensitivity, adherence to HeLa cells, and virulence of E. coli YO1. The ORF4 anti-repressor, ORF12 integrase, and ORF21 repressors that affect the lytic–lysogenic cycle of vB_EcoP_ZX5 were verified by protein overexpression. We could tell from changes of the number of total phages and the transcription level of phage genes that repressor protein is the key determinant of lytic-to-lysogenic conversion, and anti-repressor protein promotes the conversion from lysogenic cycle to lytic cycle.

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Дисертації з теми "Orf121":

Fauconnier, Aurélien. "Étude des modalités de transposition des séquences d'insertion bactériennes des familles IS91-ISCR." Electronic Thesis or Diss., Limoges, 2023. http://www.theses.fr/2023LIMO0101.

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Les éléments de la famille IS91/ISCR constituent une famille hautement associée à des gènes de virulence et de résistance aux antibiotiques. Cette famille d'IS atypique code une transposase HUH médiant les évènements de transposition en utilisant un mécanisme de transposition en cercle roulant. Contrairement aux autres membres de la famille, IS91 contient un petit ORF codant potentiellement pour un polypeptide de 121 acides aminés, Orf121, en amont du gène de la transposase, tnpA. La première partie de ce travail a consisté à une étude in silico de deux éléments de la famille IS91, IS91 et IS1294b présentent des séquences conservées au niveau de la transposase et des régions terIS et oriIS. La deuxième partie du travail a été expérimentale concernant la régulation et l’efficacité de la transposition. Nous avons montré que i) la transcription du gène tnpA provient principalement du promoteur Porf121 et que la transcription des deux gènes est couplée, ii) l'expression de la protéine Orf121 en cis ou en trans a un effet inhibiteur sur la transposition in vivo de IS91, iii) Orf121 est nécessaire à la reconnaissance et au clivage précis de l'extrémité terIS91 pour limiter la mobilisation de l’ADN adjacent. En ce qui concerne la transposition de IS91, nous avons montré que seuls les intermédiaires circulaires ADN simple brin peuvent être insérés dans une nouvelle séquence cible et avons identifié deux doigts de zinc essentiels pour l’activité de la transposition, appelés ZF1 impliquant les cystéines 41, 68, 73, 76 et ZF2 impliquant les cystéines, 53, 58, 360 et 363. Enfin, nous avons démontré que les transposases des éléments de la famille IS91 (IS91, ISKnp22) et de la famille ISCR (ISCR1) sont capables de se mobiliser et de reconnaitre et cliver l’extrémité oriIS de IS91, ISCR1 et ISCR2 et l’extrémité terIS de IS91 et ISCR2
The IS91/ISCR family is highly associated with virulence and antibiotic resistance genes. This atypical IS family encodes a HUH transposase mediating transposition events using a rolling circle transposition mechanism. Unlike the other members of the family, IS91 contains a small ORF potentially encoding a 121 amino acid polypeptide, Orf121, upstream of the transposase gene, tnpA. The first part of this work consisted of an in silico study of two members of the IS91 family, IS91 and IS1294b, that have conserved sequences of the transposase and terIS and oriIS regions. The second part of this work was experimental and focused on the transposition efficiency and regulation. We have shown that i) transcription of the tnpA gene originates mainly from the Porf121 promoter and that transcription of the two genes is coupled, ii) expression of the Orf121 protein in cis or in trans has an inhibitory effect on in vivo transposition of IS91, iii) Orf121 is required for recognition and precise cleavage of the terIS91 end to limit mobilization of the adjacent DNA. With regard to IS91 transposition, we showed that only single-stranded circular DNA intermediates can be inserted into a new target sequence and identified two zinc fingers essential for the transposase activity, called ZF1 involving cysteines 41, 68, 73 and 76 and ZF2 involving cysteines 53, 58, 360 and 363. Finally, we demonstrated that transposases from the IS91 family (IS91, ISKnp22) and the ISCR family (ISCR1) are able to mobilize and recognize and cleave the oriIS end of IS91, ISCR1 and ISCR2 and the terIS end of IS91 and ISCR2

Vieira, Débora Fernanda. "Produção e caracterização de enzimas de Streptomyces clavuligerus relacionadas com a síntese do ácido clavulânico." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-20122012-154241/.

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Ácido clavulânico (AC) é um potente inibidor de β-lactamases, produzido por Streptomyces clavuligerus, usado clinicamente em combinação com antibióticos β-lactâmicos para tratar infecções bacterianas resistentes. Apesar da produção industrial de AC já ser bem estabelecida muitos aspectos importantes relacionados com sua biossíntese permanecem carentes de estudo. Sabe-se que a via de síntese do AC envolve no mínimo 8 passos enzimáticos sendo os primeiros passos mais abordados. Por exemplo, as enzimas N2-(2-carboxietil) arginina sintase (CEAS), β-lactama sintase (BLS) e proclavaminato amidino hidrolase (PAH) são as responsáveis pela primeira, segunda e quarta reações enzimáticas respectivamente. Estudos mutagênicos recentes em S.clavuligerus relacionaram cópias extras dos genes ceas, bls e pah (ceas1, bls1 e pah1) com essa via porém nenhum ensaio enzimático foi relatado. Embora os passos finais da via ainda não estejam completamente estabelecidos, a ação de algumas enzimas putativas, como a codificada por orf12, mostraram ser essenciais a produção do AC. Assim, com o objetivo de aumentar a informação disponível sobre a biossíntese do AC estudamos quatro de seus membros: CEAS1, BLS1, PAH1 e a proteína putativa codificada pela orf12. Os genes foram isolados a partir do DNA genômico de S. clavuligerus por PCR e clonados em vetores para produção das proteínas recombinantes em E.coli. Os protocolos de expressão foram estabelecidos para CEAS1, PAH1 e ORF12 e as proteínas recombinantes foram purificadas por cromatografia de afinidade por metal. BLS foi obtida de forma isolúvel. As proteínas solúveis foram caracterizadas por meio de técnicas bioquímicas e estruturais. As análises de CEAS1 e PAH1 foram comparadas com informações já obtidas para as isozimas CEAS2 e PAH2, respectivamente. Assim, as análises de oligomerização das proteínas resultaram em uma mistura de oligômeros (monômero, dímero e tetrâmero) para CEAS1, na forma hexamérica para PAH1 e na forma dimérica para ORF12, estando de acordo com as formas solúvel e cristalográfica de CEAS2 (dímero e tetrâmero) e PAH2 (hexâmero). Espectros de dicroísmo circular mostraram que CEAS1 e PAH1 possuem um enovelamento do tipo α/β sendo estáveis até 35ºC e numa ampla faixa de pH. Os parâmetros termodinâmicos da interação entre CEAS1 e o cofator Mg+2 foram determinados mostrando que é entropicamente dirigida, com uma estequiometria de ligação de 4 : 1, com uma constante de afinidade na ordem de micromolar (KD = 1,76 ± 0.23 µM). Análises realizadas com as técnicas de reação acoplada, de Cromatografia Líquida de Alta Pressão acoplada a Espectrometria de Massas (LC-MS) e de Calorimetria de Titulação Isotérmica mostraram que CEAS1, assim como CEAS2, apresenta atividade sob o substrato gliceraldeído-3-fosfato, porém sem a formação do produto final N2-(2-carboxietil)arginina. Por outro lado, a proteína recombinante PAH1 mostrou ser inativa sobre o substrato análogo, N-α-acetil-L-arginina. Assim, apesar das isozimas manterem um padrão estrutural, podem ter mecanismos de ação distintos. Em relação a ORF12 esta proteína foi classificada com uma β-lactamase com atividade esterase de acordo com nossos estudos realizados com os substratos cefalosporina C e p-nitrofenil acetato.
Clavulanic acid (CA) is a potent inhibitor of β-lactamases, produced by Streptomyces clavuligerus, clinically used in combination with β-lactam antibiotics to treat resistant bacterial infections. Although CA industrial production is well-established, many important aspects related to its biosynthesis remains under study. It is known that CA pathway involves at least 8 enzymatic steps, being the earliest stages more addressed. For instance, N2-(2-carboxyethyl) arginine synthase (CEAS), β-lactam synthase (BLS) and proclavaminate amidinohydrolase (PAH) are responsible for the first, second and fourth enzymatic reaction, respectively. Recent mutagenic studies in S.clavuligerus have related extra copies of ceas, bls and pah genes ((ceas1, bls1 e pah1) to this pathway but none enzymatic assay was further reported. Although later stages the pathway remain unclear, the action of some putative enzymes like the codified by orf12 showed essential to CA production. Thus, aiming to increase the information available about CA biosynthesis we studied four of its members: CEAS1, BLS1, PAH1, and the putative protein codified by orf12. The genes were isolated from S.clavuligerus genomic DNA by PCR and further cloned into expression vectors in order to produce recombinant proteins in E.coli. Protocols of protein expression were established to CEAS1, PAH1 and ORF12 and recombinant proteins were purified by metal affinity chromatography. BLS was obtained as an insoluble form. Soluble proteins were characterized by means of biochemical and structural approaches. Analyses of CEAS1 and PAH1 were compared with information ever conducted to the isozymes CEAS2 and PAH2, respectively. Thus, oligomerization analysis of proteins resulted respectively in a mix of oligomers forms (monomer, dimer, tetramer) to CEAS1, hexameric form to PAH1 and dimeric form to ORF12, according to the soluble and crystallographic form of CEAS2 (dimer and tetramer) and PAH2 (hexamer). Circular Dichroism spectra showed that CEAS1 and PAH1 have an α-β conformation and were stable up to 35ºC over a wide pH range. Thermodynamic parameters of CEAS1 cofactor (Mg+2) binding were determined showing that is entropic driven, with a 4:1 binding stoichiometry, with a micro-molar affinity (KD = 1.76 ± 0.23 µM). Analyses by coupled assay, High Pressure Liquid Chromatography coupled to Mass Spectroscopy (LC-MS) and Isothermal Titration Calorimetry showed that CEAS1, as well as the CEAS2, presents activity at the substrate glyceraldehydes-3-phosphate, however without formation of final product, N2-(2-carboxyethyl)arginine. Meanwhile recombinant PAH1 showed none activity at analogous substrate, N-α-acetil-L-arginine. Thus despite isozymes maintain a structural pattern, they may have distinct action mechanism. Regards to ORF12, this protein was classified as a β-lactamase with an esterase activity according to our studies performed with the substrates cephalosporin C and p-nitrophenyl acetate.

Fang, Minggang. "The analysis of Autographa Californica multiple Nucleopolyhedrovirus EXONO (ORF141) function and its role in virus budding." Thesis, University of British Columbia, 2007. http://hdl.handle.net/2429/191.

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Baculoviruses have a biphasic replication cycle producing two types of virions, budded virus (BV) and occlusion derived virus (ODV) which are required for the systemic spread or oral infection with the insect host respectively. Little is known about the events of the BV pathway and the mechanism by which nucleocapsids are selected and directed from the nucleus to plasma membrane to form BV. The Autographa californica Multiple Nucleopolyhedrovirus (AcMNPV) gene exon0 (orf141) is known to be required for the efficient production of BV and in this study the function and mechanism by which EXON0 affects BV production was investigated. Confocal microscopic analysis showed that EXON0 localized in the nucleus in the ring zone of virogenic stroma where nucleocapsids are assembled. In addition EXON0 also concentrated in the cytoplasm at the plasma membrane. Analysis of virions revealed that EXON0 copurified with nucleocapsid fractions of both BV and ODV. In support of this yeast 2-hybrid screening, co-immunoprecipitation, and confocal microscopy revealed that EXON0 interacted with the known nucleocapsid proteins FP25 and BV/ODV-C42. Transmission electron microscopy showed that deletion of exon0 results in nucleocapsids being unable to efficiently egress from the nucleus to the cytoplasm. Cellular protein interaction analyzed by tandem affinity purification and co-immunoprecipitation showed that beta-tubulin co-purified with EXON0. Immunofluorescence also showed that EXON0 and microtubules co-localized during virus infection. The microtubule inhibitors colchicine and nocodazole affected the localization of EXON0 and significantly reduced BV production. These data support the conclusion that egress of AcMNPV nucleocapsids is facilitated by interaction of EXON0 with beta-tubulin and microtubules. Deletion and point mutation analysis mapped domains of EXON0 required for efficient budding, dimer formation and association with FP25, BV/ODV-C42 and beta-tubulin. The Leucine zipper domain was required for dimer formation, beta-tubulin and BV/ODV-C42 interaction and also reduced interaction with FP25. Multiple domains were also shown to affect BV production. This study provides a detailed analysis of EXON0 which is one of the first baculovirus genes shown to be specific for the BV pathway. The results extend our understanding of the BV pathway which is a major determinant of baculovirus pathogenesis.

Lehiy, Christopher J. "A study of two highly conserved baculovirus genes." Diss., Kansas State University, 2010. http://hdl.handle.net/2097/13892.

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Doctor of Philosophy
Department of Biology
A. Lorena Passarelli
Baculoviruses are enveloped, rod shaped viruses with circular, double-stranded DNA genomes. These viruses infect arthropods, primarily in the order Lepidoptera, although members of this virus family also infect species of Diptera, Hymenoptera, and Crustacea. The majority of these viruses undergo a bi-phasic cycle with one phase defined by the production of a budded virus (BV) form, responsible for cell to cell transmission, and the other defined by the production of an occlusion-derived virus (ODV) form, responsible for host to host transmission. The proto-typical member of the Baculoviridae family is considered to be Autographa californicaM Nucleopolyhedrovirus (AcMNPV). Its 133,894 base pair genome is predicted to encode for 156 proteins, a large number of which are essential for virus replication.. In this current work, we have further characterized two viral proteins that are highly conserved among baculoviruses. The first of these is an ortholog of the fibroblast growth factor family of proteins with sequence homology to the Drosophila Branchless protein as well as the mammalian FGF- 9, -16 and -20 subfamily. Despite its high degree of conservation among baculoviruses, the viral fibroblast growth factor (vFGF) is considered a non-essential protein, although its deletion from the genome does affect the lethality of the virus when ingested per os. In our study, we were able to localize vFGF to the membrane of BV. Its presence on the envelope affected the ability of the virus particle to bind to both heparin in vitro and to the cell surface in vivo, and may play a role in the attachment phase prior to virus entry. We also characterized AcMNPV’s open reading frame 109 (Ac-orf109). Unlike vFGF, Ac-orf109 is essential for virus replication since its deletion results in a complete lack of BV production. Transmission electron microscopy of cells transfected with an Ac-orf109 deletion virus shows the full range of virus-associated structures including mature capsid formation but there appears to be a deficiency in capsid egress out of the nucleus. Furthermore, the ODV retained in the nucleus appear to lack microvesicular membranes, an essential component for host to host transmission of infection.

Szczepańska, Agnieszka Katarzyna. "Characterisation of recombination functions of the orf12-15 DNA region from the bIL67 bacteriophage active against Lactococcus lactis subsp. Lactis." Paris 11, 2006. http://www.theses.fr/2006PA112281.

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La recombinaison homologue est essentielle au maintien de la stabilité du génome et à son évolution. Les phages possèdent des mécanismes de recombinaison particuliers qui participent efficacement à leur multiplication et à leur évolution en favorisant la circularisation et la réplication de leur ADN ainsi que les échanges horizontaux de gènes. Les études menées sur le phage bIL67 virulent pour Lactococcus lactis subsp. Lactis avaient montré que la protéine codée par l’orf13 est homologue à la recombinase Erf du phage P22. Sur la base de données de génomique comparative des phages, nous avons identifié un module putatif de recombinaison qui comprend les orfs 12 à 15. Notre travail a ensuite montré que le phage bIL67 est capable d’effectuer sa propre recombinaison homologue en l’absence de la protéine bacterienne RecA. Nous avons aussi montré que ce phage inhibe l’activité exonucléase et celle de recombinaison du complexe RexAB chez L. Lactis. La caractérisation biochimique de la protéine codée par l’orf14 a montré que c’est une protéine de type SSB. Par des expériences de retard de migration en gel il a été démontré que Orf14 est capable de se lier d’une manière séquence non spécifique aux acides nucléiques simple brin. La modélisation de la structure tridimensionnelle de la protéine, combinée à une analyse in vitro de protéines mutantes purifiées, indique que le domaine de liaison à l’ADN de la protéine se situe dans un domaine OB putatif (oligosaccharide/oligonucleotide binding), une caractéristique partagée par toutes les protéines SSB. De plus, Orf14 est capable de complémenter partiellement in vivo le défaut de croissance d’un mutant ssb thermosensible d’E. Coli
Recombination is involved in key stages of phage multiplication. Due to its importance for phage development, it is postulated that phages do not rely only on host-encoded functions but posses their own recombination proteins. Despite general indications that lactococcal phages can recombine, existence of recombination systems lacked direct proof. Results of this thesis supply evidence of phage bIL67-mediated recombination and studies that led to characterising individual recombination genes in the phage genome. This work presents in a general way that phage bIL67 encodes proteins proficient in promoting recombination. Phage bIL67 was shown to develop and recombine independently of host RecA protein, which suggested the presence of phage-encoded proteins promoting the same events as bacterial recombination proteins. The putative recombination region was defined to orfs12-15 of the early transcribed part of the phage genome. Finding orf13 gene product to be homologous to phage P22 Erf recombinase and an overall organisation similarity with P22 recombination module were the initial evidences suggesting localisation of the putative recombination gene cluster in this region. Identification of orf14-encoded SSB function just upstream of orf13 supplied further proof. Putative recombination region was shown to encode anti-Exo activity acting against main lactococcal Exo protein (RexAB). Molecular characterisation of Orf14 activity in vitro confirmed its affinity for single-stranded nucleic acids. Amino acid substitution of Orf14 led to identification of the functional domains involved in DNA binding and protein-protein interactions. In vivo complementation studies proved Orf14 to partially substitute SSB of E. Coli

Stockmeyer, Kerstin [Verfasser]. "Heterologe Expression und Funktionsanalyse des CMS-assoziierten offenen Leserahmens orf107 aus Sorghum bicolor in Nicotiana tabacum und Arabidopsis thaliana / Kerstin Stockmeyer." Kiel : Universitätsbibliothek Kiel, 2008. http://d-nb.info/1019543426/34.

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Okada, Sachiko. "Characterization of the repeat-rich region of the Y chromosome harboring a male-specific gene, ORF162, in the liverwort, Marchantia polymorpha." Kyoto University, 2002. http://hdl.handle.net/2433/149933.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第9648号
農博第1276号
新制||農||847(附属図書館)
学位論文||H14||N3680(農学部図書室)
UT51-2002-G406
京都大学大学院農学研究科応用生命科学専攻
(主査)教授大山莞爾, 教授佐藤文彦, 教授關谷次郎
学位規則第4条第1項該当

Diekmann, Ulrike Verfasser], and Lothar [Akademischer Betreuer] [Jänsch. "Characterisation of the KSHV protein ORF20 and its role in innate immunity / Ulrike Diekmann ; Betreuer: Lothar Jänsch." Braunschweig : Technische Universität Braunschweig, 2019. http://d-nb.info/1179909984/34.

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Diekmann, Ulrike [Verfasser], and Lothar [Akademischer Betreuer] Jänsch. "Characterisation of the KSHV protein ORF20 and its role in innate immunity / Ulrike Diekmann ; Betreuer: Lothar Jänsch." Braunschweig : Technische Universität Braunschweig, 2019. http://d-nb.info/1179909984/34.

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C, G. GUALBERTO JOSE MANUEL. "Etude des genes nad3, rps12, orf156 et coxiii du genome mitochondrial du ble (triticum aestivum) : structure, expression et edition de leurs transcrits." Strasbourg 1, 1990. http://www.theses.fr/1990STR13091.

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Deux regions transcrites du genome mitochondrial du ble ont ete sequencees et analysees. Une de ces regions correspond a une unite de transcription contenant les genes nad3 et rps12, codant respectivement pour la sous-unite 3 de la nadh deshydrogenase et pour la proteine ribosomale s12. Ces deux genes sont precedes par un gene de trna (trns-gcu) et par un pseudo-gene de trna. Trois phases ouvertes de lecture, orf299, orf86 et orf156, sont presentes en aval de rps12. Des anticorps specifiques detectent in vivo les produits de rps12 et de l'orf156. L'autre region etudiee contient les genes coxiii et trne-uuc, codant respectivement pour la sous-unite 3 de la cytochrome oxydase et pour un trna#g#l#u. La transcription de ces sequences a ete etudiee. L'analyse de l'utilisation des codons cgg dans nad3, rps12 et coxiii a permis de demontrer qu'une activite d'edition du rna, qui remplace specifiquement des cytidines en uridines, existe dans les mitochondries vegetales. Les sites d'edition dans les genes nad3, rps12, orf299, orf156 et coxiii ont ete determines. Des similarites de sequence autour des sites d'edition suggerent que des rna anti-sens sont impliques dans la specificite de l'edition. Des clones partiellement edites de nad3 et rps12 ont ete identifies. L'ensemble des resultats montre que l'edition est un processus post-transcriptionnel qui permet la conservation des proteines mitochondriales chez les plantes superieures

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Книги з теми "Orf121":

Meng, X. J. Hepatitis E virus. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0048.

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Hepatitis E virus (HEV) is a small, non-enveloped, single-strand, positive-sense RNA virus of approximately 7.2 kb in size. HEV is classified in the family Hepeviridae consisting of four recognized major genotypes that infect humans and other animals. Genotypes 1 and 2 HEV are restricted to humans and often associated with large outbreaks and epidemics in developing countries with poor sanitation conditions, whereas genotypes 3 and 4 HEV infect humans, pigs and other animal species and are responsible for sporadic cases of hepatitis E in both developing and industrialized countries. The avian HEV associated with Hepatitis-Splenomegaly syndrome in chickens is genetically and antigenically related to mammalian HEV, and likely represents a new genus in the family. There exist three open reading frames in HEV genome: ORF1 encodes non-structural proteins, ORF2 encodes the capsid protein, and the ORF3 encodes a small phosphoprotein. ORF2 and ORF3 are translated from a single bicistronic mRNA, and overlap each other but neither overlaps ORF1. Due to the lack of an efficient cell culture system and a practical animal model for HEV, the mechanisms of HEV replication and pathogenesis are poorly understood. The recent identification and characterization of animal strains of HEV from pigs and chickens and the demonstrated ability of cross-species infection by these animal strains raise potential public health concerns for zoonotic HEV transmission. It has been shown that the genotypes 3 and 4 HEV strains from pigs can infect humans, and vice versa. Accumulating evidence indicated that hepatitis E is a zoonotic disease, and swine and perhaps other animal species are reservoirs for HEV. A vaccine against HEV is not yet available.

Частини книг з теми "Orf121":

Ramos, Kenneth S., Stefano Guerra, and Randa El-Zein. "Precision Medicine Approaches for Stratification and Development of Novel Therapies of Latin(x) Patients at Risk of Lung Malignancy." In Advancing the Science of Cancer in Latinos, 89–98. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-14436-3_8.

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AbstractPrecision medicine has emerged as an optimal health-care delivery platform, which emphasizes integration of individual patient characteristics into patient care. For lung cancer, precision approaches have focused mostly on targeted therapies directed at tyrosine kinases and immunotherapy. It is proposed that refinements should focus on improved risk stratification of patients at heightened risk of lung malignancy, namely patients with chronic obstructive pulmonary disease (COPD). African ancestry is associated with worsened clinical outcomes in COPD and lung cancer, which is relevant for Latinx populations given that varying degrees of African ancestry exist among several Latinx subgroups. The work reviewed here focuses on ORF1p, a protein encoded by Long Interspersed Element-1 (LINE-1) and associated with genetic instability. Because high expression of ORF1p is associated with poor prognosis in patients with non-small-cell lung cancer (NSCLC), it is hypothesized that circulating ORF1p can be monitored as a proxy of genetic instability in patients with COPD and lung cancer. Circulating ORF1p levels correlate with FEV1 deficits and airflow limitation (the hallmark of COPD) in former smokers, and tissue expression of ORF1p is increased in TP53 mutant NSCLC compared to wildtype. Understanding the role of ORF1p in COPD and lung cancer and its utility as a biomarker of genetic instability may lead to advances in lung cancer care and development of novel targeted therapies.

Schwindt, Joel. "The mystical architecture of Orfeo1." In Orpheus in the Academy, 172–213. London: Routledge, 2021. http://dx.doi.org/10.4324/9781003029816-7.

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Deming, Damon J., Rachel L. Graham, Mark R. Denison, and Ralph S. Baric. "MHV-A59 Orf1a Replicase Protein NSP7-NSP10 Processing in Replication." In Advances in Experimental Medicine and Biology, 101–4. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/978-0-387-33012-9_17.

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Kumar, Amit, Ajit Kumar Saxena, Gwo Giun (Chris) Lee, Amita Kashyap, and G. Jyothsna. "Physiochemical Characterization and Domain Annotation of ORF1ab Polyprotein of Novel Corona Virus 19." In Novel Coronavirus 2019, 23–35. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-7918-9_4.

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Wilde, Annegret, Hendrik Schubert, Heiko Härtel, and Thomas Börner. "Alterations of the Photosynthetic Apparatus in a Synechocystis sp. PCC 6803 ORF184 Mutant." In Photosynthesis: from Light to Biosphere, 2437–40. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_574.

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Naufer, M. Nabuan, and Mark C. Williams. "Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy." In Methods in Molecular Biology, 283–97. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-0716-0231-7_18.

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Liu, D. X., S. Shen, H. Y. Xu, and T. D. K. Brown. "Proteolytic Processing of the Polyprotein Encoded by ORF1b of the Coronavirus Infectious Bronchitis Virus (IBV)." In Advances in Experimental Medicine and Biology, 149–59. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5331-1_20.

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Hughes, Scott A., Mark R. Denison, Pedro Bonilla, Julian L. Leibowitz, Ralph S. Baric, and Susan R. Weiss. "A Newly Identified MHV-A59 ORF1a Polypeptide p65 is Temperature Sensitive in Two RNA Negative Mutants." In Coronaviruses, 221–26. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2996-5_35.

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Liu, D. X., K. W. Tibbles, D. Cavanagh, T. D. K. Brown, and I. Brierley. "Involvement of Viral and Cellular Factors in Processing of Polyprotein Encoded by ORF1a of the Coronavirus IBV." In Advances in Experimental Medicine and Biology, 413–21. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1899-0_67.

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Cui, Guanglei, Xue Li, Ning Yu, and Kenneth M. Merz. "Interpreting The Observed Substrate Selectivity And The Product Regioselectivity In Orf2-Catalyzed Prenylation From X-Ray Structures." In Challenges and Advances in Computational Chemistry and Physics, 351–75. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-1-4020-9956-4_13.

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Тези доповідей конференцій з теми "Orf121":

Hajighasemi, F., and A. Shirkavand. "Insilico study of COVID-19 ORF1ab protein allergenicity." In ERS International Congress 2022 abstracts. European Respiratory Society, 2022. http://dx.doi.org/10.1183/13993003.congress-2022.3367.

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Stoicescu, Ramona, Razvan-Alexandru Stoicescu, Codrin Gheorghe, Adina Honcea, and Iulian Bratu. "CONSIDERATIONS ON SARS-COV-2 DIAGNOSIS IN THE LABORATORY OF UNIVERSITY EMERGENCY CLINICAL HOSPITAL OF CONSTANTA." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/07.

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Coronaviruses are members of the Coronaviridae family. They are enveloped, non-segmented, positive-sense, single-stranded RNA viruses. Their genome size is about 30 kilobases (kb) which consist, at the 5’ end, of non-structural open reading frames (ORFs: ORF1a, ORF 1b) which code for 16 non structural proteins, and at the 3’ end the genes which code for four structural proteins including membrane (M), envelope (E), spike (S), and nucleocapsid (N) proteins. Due to the rapid spread of COVID-19, a reliable detection method is needed for patient diagnosis especially in the early stages of the disease. WHO has recommended nucleic acid amplification tests such as real-time reverse transcription-polymerase chain reaction (RT-PCR). The assay detects three SARS-CoV-2 RNA targets: the envelope (E) gene, the nucleocapsid (N) gene and a region of the open reading frame (ORF1) of the RNA-dependent RNA polymerase (RdRp) gene from SARS-CoV-2 virus isolate Wuhan-Hu-1. Our study was made in the first 3 months of the year 2021 using the real-time RT PCR results obtained in the Cellular Biology ward of the University Emergency Clinical Hospital. In our lab we are testing the inpatients from the hospital wards (Neurology, Pediatrics, Surgery, Internal medicine, ICU, Cardiology, etc.); we are also testing the outpatients from Dialysis and Oncology, 2 days prior to their therapy; we also test the health care personnel. The number of tests we performed was: in January 1456, with 399 positive results (27.4%), 33 deaths; in February 1273 tests, 221 positive (17.36%), 16 deaths; in March 1471 tests, 373 positive (25.36%), 37 deceased.

Thomas, Michael A., Rui Song, and Marjorie Robert-Guroff. "Abstract 472: Effects of the deletion of early region 4 (E4) open reading frame 1 (orf1), orf1-2, orf1-3 and orf1-4 on virus-host cell interaction, transgene expression, and immunogenicity of replicating adenovirus vaccine vectors." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-472.

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Pontes dos Santos, Luiz Henrique, Jannison Karlly Cavalcante Ribeiro, Juliana Osorio Alves, Maria Izabel Florindo Guedes, Stela Mirla da Silva Felipe, Raquel Martins de Freitas, Paula Matias Soares, et al. "SARS-CoV-2 variant strains and viral phylodynamics of ORF1a-1b genetic aspects in South America." In 2021 International Conference on Computational Science and Computational Intelligence (CSCI). IEEE, 2021. http://dx.doi.org/10.1109/csci54926.2021.00034.

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Yang, Fan, Hong-Liang Liu, Xiao-Wan Liu, Bo Guo, Song Li, Ping Li, and Ling Zhug. "Development of a TTSuV2-ORF1 Protein-based Indirect Blocking ELISA for Serological Testing." In 2015 International Conference on Medicine and Biopharmaceutical. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789814719810_0023.

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Звіти організацій з теми "Orf121":

Mawassi, Munir, and Valerian Dolja. Role of RNA Silencing Suppression in the Pathogenicity and Host Specificity of the Grapevine Virus A. United States Department of Agriculture, January 2010. http://dx.doi.org/10.32747/2010.7592114.bard.

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RNA silencing is a defense mechanism that functions against virus infection and involves sequence-specific degradation of viral RNA. Diverse RNA and DNA viruses of plants encode RNA silencing suppressors (RSSs), which, in addition to their role in viral counterdefense, were implicated in the efficient accumulation of viral RNAs, virus transport, pathogenesis, and determination of the virus host range. Despite rapidly growing understanding of the mechanisms of RNA silencing suppression, systematic analysis of the roles played by diverse RSSs in virus biology and pathology is yet to be completed. Our research was aimed at conducting such analysis for two grapevine viruses, Grapevine virus A (GVA) and Grapevine leafroll-associated virus-2 (GLRaV- 2). Our major achievements on the previous cycle of BARD funding are as follows. 1. GVA and GLRaV-2 were engineered into efficient gene expression and silencing vectors for grapevine. The efficient techniques for grapevine infection resulting in systemic expression or silencing of the recombinant genes were developed. Therefore, GVA and GLRaV-2 were rendered into powerful tools of grapevine virology and functional genomics. 2. The GVA and GLRaV-2 RSSs, p10 and p24, respectively, were identified, and their roles in viral pathogenesis were determined. In particular, we found that p10 functions in suppression and pathogenesis are genetically separable. 3. We revealed that p10 is a self-interactive protein that is targeted to the nucleus. In contrast, p24 mechanism involves binding small interfering RNAs in the cytoplasm. We have also demonstrated that p10 is relatively weak, whereas p24 is extremely strong enhancer of the viral agroinfection. 4. We found that, in addition to the dedicated RSSs, GVA and GLRaV-2 counterdefenses involve ORF1 product and leader proteases, respectively. 5. We have teamed up with Dr. Koonin and Dr. Falnes groups to study the evolution and function of the AlkB domain presents in GVA and many other plant viruses. It was demonstrated that viral AlkBs are RNA-specific demethylases thus providing critical support for the biological relevance of the novel process of AlkB-mediated RNA repair.