Дисертації з теми "Optical Molecular Sensors"
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Lubian, Elisa. "Porphyrin Derivatives as Optical Molecular Sensors." Doctoral thesis, Università degli studi di Padova, 2011. http://hdl.handle.net/11577/3427495.
Повний текст джерелаIl progetto di ricerca di questa Tesi di Dottorato ha riguardato la sintesi, la caratterizzazione e lo studio delle proprietà di ricognizione molecolare di nuovi derivati bis-porfirinici contenenti un ponte triazinico. Studi di spettroscopia UV-vis hanno permesso di determinare l’affinità di tali recettori nei confronti delle diammine lineari di formula generale H2N(CH2)nNH2, con n = 4-8. Le costanti di formazione dei complessi host-guest sono molto grandi, fino a 10e7 M-1, grazie all’effetto ditopico realizzato dai due centri porfirinici. La coordinazione delle diammine al dimero porfirinico è associata ad una variazione marcata del colore e questo fatto ha favorito l’impiego di tali derivati in ambito sensoristico. A tal proposito, è stata messa a punto una procedura per supportare i dimeri porfirinici su materiali polimerici per la costruzione di sensori da utilizzare per l’analisi in flusso continuo. Questi derivati sono stati anche utilizzati come pinze molecolari (tweezers) per la determinazione della configurazione assoluta di molecole chirali (diammine, ammino esteri, ammino ammidi e ammino alcoli, monoalcoli secondari) mediante l’impiego della spettroscopia di dicroismo circolare (CD), in collaborazione con la Prof. Berova della Columbia University. Parallelamente, sono stati realizzati studi di deposizione di derivati porfirinici su superfici in vista di applicazioni di tipo sensoristico e in campo energetico (fotovoltaico). La caratterizzazione dei substrati è stata condotta mediante misure di microscopia elettronica a scansione (SEM), microscopia a forza atomica (AFM) e microscopia a scansione ad effetto tunnel (STM). Questi studi di deposizione hanno dimostrato come, scegliendo le opportune condizioni di deposizione, sia possibile costruire dei sistemi ordinati a lungo raggio, su superfici di diversa natura, rendendo questi sistemi candidati ideali per lo sviluppo di nuovi materiali ad alto contenuto tecnologico.
Sgarzi, Massimo <1984>. "Characterization of optical transduction-based molecular systems and nanoparticles for the development of chemical sensors." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6314/4/Sgarzi_Massimo_tesi.pdf.
Повний текст джерелаSgarzi, Massimo <1984>. "Characterization of optical transduction-based molecular systems and nanoparticles for the development of chemical sensors." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6314/.
Повний текст джерелаLo, Presti Maria. "Optical detection of chemical species of environmental and biological relevance using molecular sensors and hybrid materials." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/172664.
Повний текст джерела[CA] La present tesi doctoral titulada "Detecció òptica d'espècies químiques d'importància ambiental i biològica utilitzant sensors moleculars i materials híbrids" se centra en el disseny, preparació, caracterització i avaluació de sensors químics moleculars. El treball realitzat es pot dividir en dues parts: (i) síntesi de sensors de cations metàl·lics en dissolució i (ii) síntesi i caracterització de nanopartícules híbrides orgànic-inorgànics per al reconeixement d'espècies químiques i biològiques. En el primer capítol s'introdueix el marc en el qual s'engloben els fonaments teòrics de la química supramolecular en què es basen els estudis pràctics realitzats durant la present tesi doctoral. A continuació, en el capítol dos, es presenten els objectius generals de la tesi. En el tercer capítol es presenta un quimiodosímetro crom-fluorogénic, capaç de detectar selectivament cations trivalents entre cations i anions monovalents i divalents mitjançant una reacció de deshidratació en aigua. En el quart capítol es presenta una unitat (BODIPY) connectada electrònicament amb un macrocicle dithia-dioxa-aza. Les solucions de acetonitril i aigua-acetonitril 95:5 v/v de la sonda van mostrar una banda ICT a la zona visible i són gairebé no emisivas. Quan es va utilitzar acetonitril com a dissolvent, l'addició de Hg (II) i cations metàl·lics trivalents va induir un canvi hipsocròmic de la banda d'absorció i millores moderades de l'emissió. Es va obtenir una resposta altament selectiva a l'utilitzar mitjans competitius com aigua-acetonitril 95:5 v/v. En aquest cas, només el Hg (II) va induir un canvi hipsocròmic de la banda d'absorció i una millora marcada de l'emissió. El cinquè capítol explora el desenvolupament de sensors per berberina i amantadina. Dues molècules d'interès biològic pel seu ús com a fàrmacs. S'han preparat tres sistemes de sensors basats en l'aproximació de portes moleculars. En concret, sobre nanopartícules MCM-41 carregades amb rodamina B com a unitat de senyalització, s'ha dut a terme la funcionalització amb diverses amines i el bloqueig de porus amb cucurbituril CB7. Les amines utilitzades són ciclohexilamina, bencilamina i amantadina. Els materials obtinguts s'han caracteritzat per tècniques iv de difracció de raigs X i microscòpia electrònica de transmissió confirmant l'estructura mesoporosa de les nanopartícules. Els materials preparats mostren una resposta a la berberina i la adamantina, llevant el tap i alliberant el tint fluorescent a l'mig. La resposta dels materials a les dues substàncies d'interès (berberina i amantadina) depèn de l'estructura química de cada un dels materials en funció de les constants d'afinitat entre l'anàlit i CB7. Els resultats obtinguts obren el camí a l'ús de portes moleculars com a sensors de berberina i amantadina.
[EN] The present doctoral thesis entitled "Optical detection of chemical species of environmental and biological importance using molecular sensors and hybrid materials" focuses on the design, preparation, characterization and evaluation of molecular chemical sensors. The work carried out can be divided into two parts: (i) synthesis of metal cation sensors in solution and (ii) synthesis and characterization of hybrid organic-inorganic nanoparticles for the recognition of chemical and biological species. The first chapter introduces the framework that encompasses the theoretical foundations of supramolecular chemistry on which the practical studies carried out during this doctoral thesis are based. Next, in chapter two, the general objectives of the thesis are presented. In the third chapter, a chromium-fluorogenic chemodosimeter is presented, capable of selectively detecting trivalent cations by means of a dehydration reaction in water. The fourth chapter presents a new compound containing a BODIPY unit electronically connected with a dithia-dioxa-aza macrocycle. Acetonitrile and water-acetonitrile 95:5 v/v solutions of the probe showed an ICT band in the visible zone and were nearly non-emissive. When acetonitrile was used as a solvent, addition of Hg(II) and trivalent metal cations induced an hypsochromic shift of the absorption band and moderate emission enhancements. A highly selective response was obtained when using competitive media such as water- acetonitrile 95:5 v/v. In this case only Hg(II) induced a hypsochromic shift of the absorption band and a marked emission enhancement. The fifth chapter explores the development of sensors for berberine and amantadine; two molecules of biological interest due to their use as drugs. Three sensing systems based on a "molecular gate" approximation have been prepared. Specifically, MCM-41 nanoparticles were loaded with Rhodamine B as a signalling unit, functionalized with various amines and capped with cucurbituril CB7. The amines used are cyclohexylamine, benzylamine and amantadine., The materials obtained were characterized by X-ray diffraction techniques and transmission vi electron microscopy, confirming the mesoporous structure of the nanoparticles. The prepared materials showed a response to berberine and adamantine, which induced release of the fluorescent dye to the medium. The response of the materials to the two substances of interest (berberine and amantadine) depends on the chemical structure of the capping ensemble and it is a function of the affinity constants between the analyte and CB7. The results obtained open the way to the use of gated materials as berberine and amantadine probes.
We thank the Spanish Government (MAT2015-64139-C4-1-R) and Generalitat Valenciana (PROMETEOII/2014/047). M. L. P. thanks Generalitat Valenciana for her Grisolia fellowship. Thanks are also due to Fundação para a Ciência e Tecnologia (Portugal) for financial support to the Portuguese NMR network (PTNMR, Bruker Avance III 400-Univ. Minho), FCT and FEDER (European Fund for Regional Development)-COMPETEQREN- EU for financial support to the research centre CQ/UM [PEst-C/ QUI/UI0686/2013 (FCOMP-01-0124-FEDER-037302)], and a post- doctoral grant to R. M. F. Batista (SFRH/BPD/79333/2011).
Lo Presti, M. (2021). Optical detection of chemical species of environmental and biological relevance using molecular sensors and hybrid materials [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/172664
TESIS
Shcherbakova, Elena G. "Implementation of High Throughput Screening Strategies in Optical Sensing for Pharmaceutical Engineering." Bowling Green State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510758614142002.
Повний текст джерелаSantos, Figueroa Luis Enrique. "New approaches for the development of chromo-fluorogenic sensors for chemical species of biological, industrial and environmental interest." Doctoral thesis, Universitat Politècnica de València, 2015. http://hdl.handle.net/10251/43216.
Повний текст джерелаSantos Figueroa, LE. (2014). New approaches for the development of chromo-fluorogenic sensors for chemical species of biological, industrial and environmental interest [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/43216
TESIS
Premiado
Alfeeli, Bassam. "Miniature gas sensing device based on near-infrared spectroscopy." Thesis, Virginia Tech, 2005. http://hdl.handle.net/10919/35911.
Повний текст джерелаMaster of Science
Lynch, Patrick Lionel Mark. "Fluorescent molecular sensors for pH." Thesis, Queen's University Belfast, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317479.
Повний текст джерелаChien, Wei-Yin. "Electromagnetic modeling and experimental evaluation of plasmon-based molecular sensors." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21949.
Повний текст джерелаL'avancée des nanosciences et de la nanotechnologie au cours des dernières décennies a suscité un renouvellement de l'intérêt pour les propriétés optiques des métaux. Aujourd'hui, la Plasmonique cherche à contrôler les champs proches électromagnétiques des nanostructures métalliques afin de bénéficier des nouvelles applications reposant sur l'optique de sous-longueur d'onde. En particulier, ce mémoire explore l'utilisation de la résonance à plasmons pour la capture des molécules. L'étude se divise en la résonance à plasmons de surface (SPR) pour des surfaces métalliques planes et pour des nanosphères métalliques. Ces deux méthodes permettent de créer des capteurs sensibles aux variations d'indice de réfraction et d'autres qui reposent sur des effets non-linéaires tels que la diffusion Raman exaltée de surface (SERS). Suite à l'introduction des bases, l'opération ainsi que des résultats expérimentaux de deux capteurs à plasmons de surface sont présentés. Un capteur intégré et une version 2D avec modulation de longueur d'onde et de l'angle possèdent une sensibilité angulaire d'environ 126°/RIU et 91°/RIU, respectivement. Par la suite, la réalisation de la méthode du multiple-multipole, utile pour évaluer l'efficacité de la concentration des champs entre des nanosphères métalliques, est discutée. Une amélioration de la concentration de champ optique de l'ordre de 450 par des nanospheres d'or est présentée. Mots-clés: plasmon de surface, plasmonique, biocapteurs optiques, diffusion Raman exaltée de surface, optique des métaux, nanophotonique, nanomatériel, électro-dynamique classique
Wan, Wei. "Molecularly imprinted chromogenic and fluorogenic receptors as optical sensor matrix." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2015. http://dx.doi.org/10.18452/17261.
Повний текст джерелаThis dissertation derives from the DFG project aimed on preparing optical sensor material for anionic target through combing the outstanding recognition of Molecularly imprinted polymer (MIPs) and sensitive fluorescence technique. A single step direct sensing strategy is adopted to prepare the sensor material in this thesis. Here, a fluorescence probe is covalently embedded into the MIP cavity for signal transduction. MIP sensor material are prepared in forms of bulk, thin film and particles. The material is characterized using various techniques. The performance of the sensor materials is also assessed in terms of sensibility, selectivity as well response time. Both the switching on and off signaling methods are tested in this thesis. The prepared material achieves the goal of the project. Both the prepared thin film as well as core-shell particle show prominent selectivity even a strong enantioselective discrimination. These sensing materials also have low LOD to 60 µM and fast sensing response of 20 seconds. Especially the core-shell sensing particle can be coupled with various detection techniques and is potentially applicable for developing miniaturized sensing instrument for on-line detection as well as point of care diagnose.
Azzi, Victor, and Victor Azzi. ""Sensor-in-fibre" optical probes for molecular sensing in the gastrointestinal tract of murine models." Master's thesis, Université Laval, 2019. http://hdl.handle.net/20.500.11794/37631.
Повний текст джерелаL’obésité et les maladies cardiométaboliques sont des problèmes de santé publique dans les populations nordiques du Canada ainsi qu’à travers le monde. Il est actuellement proposé que l’augmentation de ces désordres est en partie causée par divers facteurs environnementaux qui génèrent des changements importants du microbiote intestinal. Cette communauté microbienne qui peuple notre tractus gastrointestinal joue un rôle clé dans le métabolisme de nutriments, mais peut aussi avoir des effets néfastes lorsque son équilibre avec l’hôte est perturbé. Cette compréhension a mis en évidence le manque d’outils prédictifs permettant un diagnostic rapide et efficace dans le domaine biomédical. L’analyse actuelle du microbiote est réalisée à posteriori au niveau des selles, ce qui requiert du personnel hautement qualifié de même que des procédures longues et dispendieuses. L’objectif de ce projet est de concevoir un capteur optique qui, une fois implanté dans l’intestin, permettra de détecter en temps réel des biomarqueurs clés produit par le microbiome intestinal. Dans le cadre d’une preuve de concept, une architecture fibrée simple permettant de mesurer quantitativement des variations de pH est démontrée. Contrairement aux capteurs fibrés traditionnels, la sonde optique de ce projet exploite l’onde évanescente générée sur la périphérie de l’interface pour exciter des nanomatériaux greffés dont les propriétés de fluorescence varient selon leur environnement chimique. Les mesures sont possibles grâce à un système optique mobile contrôlé par un logiciel convivial qui permet à un utilisateur nonexpert d’utiliser l’appareil. Les résultats confirment qu’avec un étalonnage préalable il est possible avec cette sonde modèle de prendre des mesures quantitatives du pH en temps réel in vitro. Les expériences préliminaires suggèrent que la sonde permet aussi de mesurer le pH en temps réel dans l’intestin in vivo.
Obesity and cardiometabolic diseases (CMD) are major public health issues among Canada’s northern population and throughout the world. It is believed that the exponential rise in CMD incidence is due to numerous environmental factors, which are driving important changes in the gut microbiome. This microbial community which populates our intestinal tract plays a key role in nutrient and energy metabolism, but can also drive pathogenic mechanisms when its interaction with the host is disrupted. This understanding has highlighted the lack of predictive tools and biomarkers for rapid and efficient diagnostic of various diseases within the medical field. Current analysis of the gut microbiota is mostly based on sequencing technologies to determine microbial composition and gene expression, while functional analyses are limited to surrogate markers of microbial activities through stool metabolites. The goal of this study is to develop a “Sensor-in-Fibre” probe with the capacity to detect key microbiome-derived molecules relevant to CMD pathogenesis in real time in vivo. The optical probe takes advantage of evanescent fields generated on its peripheral interface to excite species-selective surface-grafted sensing nanomaterials that have varying fluorescent properties based on the target molecules present in the surrounding environment. As a model system, FITC functionalized with (3-aminopropyl)triethoxysilane was grafted on the periphery of an optical fiber, leading to qualitative pH measurements revealed through fluorescence emission qualities. These measurements are possible due to the use of a mobile signal collection apparatus in conjunction with custom software made to enable a non-expert technician to use it. The experimental results demonstrate that, with the appropriate preparation, it is possible to quantitatively measure pH with this probe structure in vitro and preliminary studies suggest that the probe is also capable of measuring pH in vivo in real time.
Obesity and cardiometabolic diseases (CMD) are major public health issues among Canada’s northern population and throughout the world. It is believed that the exponential rise in CMD incidence is due to numerous environmental factors, which are driving important changes in the gut microbiome. This microbial community which populates our intestinal tract plays a key role in nutrient and energy metabolism, but can also drive pathogenic mechanisms when its interaction with the host is disrupted. This understanding has highlighted the lack of predictive tools and biomarkers for rapid and efficient diagnostic of various diseases within the medical field. Current analysis of the gut microbiota is mostly based on sequencing technologies to determine microbial composition and gene expression, while functional analyses are limited to surrogate markers of microbial activities through stool metabolites. The goal of this study is to develop a “Sensor-in-Fibre” probe with the capacity to detect key microbiome-derived molecules relevant to CMD pathogenesis in real time in vivo. The optical probe takes advantage of evanescent fields generated on its peripheral interface to excite species-selective surface-grafted sensing nanomaterials that have varying fluorescent properties based on the target molecules present in the surrounding environment. As a model system, FITC functionalized with (3-aminopropyl)triethoxysilane was grafted on the periphery of an optical fiber, leading to qualitative pH measurements revealed through fluorescence emission qualities. These measurements are possible due to the use of a mobile signal collection apparatus in conjunction with custom software made to enable a non-expert technician to use it. The experimental results demonstrate that, with the appropriate preparation, it is possible to quantitatively measure pH with this probe structure in vitro and preliminary studies suggest that the probe is also capable of measuring pH in vivo in real time.
Résumé en espagnol
Résumé en espagnol
Marie, Héléne. "Elaboration of a new sensor based on molecularly imprinted polymers for the detection of molecules in physiological fluids." Phd thesis, Université de Technologie de Compiègne, 2013. http://tel.archives-ouvertes.fr/tel-00977390.
Повний текст джерелаDavoudzadeh, Gholami Mahnaz. "Highly sensitive materials and sensors for the detection of bioactive molecules." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/227476/1/Mahnaz_Davoudzadeh%20Gholami_Thesis.pdf.
Повний текст джерелаBompart, Marc. "Molecularly imprinted polymers and nano-composites by free radical and controlled/living radical polymerization : applications in optical sensors." Compiègne, 2010. http://www.theses.fr/2010COMP1870.
Повний текст джерелаThis thesis is organized in three chapters and is based on three published papers, and two manuscripts about to be submitted. Molecularly imprinted polymers (MIPs) are tailor-made synthetic receptors that are obtained by polymerization in the presence of a molecular template. The first paper describes the use of Raman spectroscopy to detect and quantify the presence of the imprinting template in single molecularly imprinted polymer microspheres. The polymers were imprinted with the Beta-blocking drugs propranolol and atenolol, and precipitation polymerization was used to obtain spherical particles. The nanoparticles were used for bulk detection whereas with micrometer-sized particles, quantitative measurements on single particles were possible. Relatively low detection limits down to 1µM have been reached for the detection of S-propranolol through bulk measurements on MIP nanoparticles. The second paper describes chemical nanosensors with a submicron core-shell composite design, based on a polymer core, a molecularly imprinted polymer (MIP) shell for selective analyte recognition, and an interlayer of gold nanoparticles for signal amplification. SERS measurements on single nanosensors yielded a detection limit of 10-7 M for the Beta-blocker propranolol, several orders of magnitude lower than on plain MIP spheres. These particles were also used as sensor materials with localized surface plasmon resonance measurements as the transduction method (Paper III), for the determination of the Beta-blocking drug propranolol. The sensors were used in suspension and were measured using a standard UV-Vis spectrophotometer. In order to solve general problems associated with MIPs, in particular their heterogeneity in terms of inner morphology and distribution of binding site affinities, it has been suggested to use modern methods of controlled/living radical polymerization for their synthesis. This also facilitates their generation in the form of nanomaterials, nanocomposites, and thin films, a strong recent trend in the field. The fourth paper reviews recent advances in the molecular imprinting area, with special emphasis on the use of controlled polymerization methods, their benefits, and current limitations. In the last paper, we have for the first time used a recently developed CRP method based on iodide mediated polymerization, reversible chain transfer catalyzed polymerization (RTCP), for the synthesis of MIPs. We show on the example of MIPs specific for the Beta-blocking drug propranolol that RTCP is compatible with MIP synthesis, both for the synthesis of bulk polymers and nanospheres, and that it yields polymers with the same binding capacity as the standard FRP method used for comparison. Solid-state NMR measurements revealed that the conversion of pendant vinyl groups was higher with RTCP than with polymers synthesized by FRP, in particular at higher initiator concentrations
FARINA, DONATELLA. "Research and development of innovative molecular sensor technologies for the monitoring of volatile organic compounds in wines." Doctoral thesis, Università degli studi della Basilicata, 2021. http://hdl.handle.net/11563/146584.
Повний текст джерелаTon, Xuan-Anh. "Fiber optic chemical sensors based on molecularly imprinted polymers for the detection of mycotoxins." Phd thesis, Université de Technologie de Compiègne, 2013. http://tel.archives-ouvertes.fr/tel-01002117.
Повний текст джерелаTipparaju, Venkata Satya Sai Sarma. "An active core fiber optic gas sensor using a photonic crystal hollow core fiber as a transducer." Master's thesis, Mississippi State : Mississippi State University, 2007. http://sun.library.msstate.edu/ETD-db/theses/available/etd-06262007-164352/.
Повний текст джерелаBeam, Brooke Michelle. "Waveguide Sensor Platforms: A) Development of the Electroactive Fiber-Optic Chip and B) Attenuated Total Reflectance Spectroscopy of New Molecular Materials." Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/194142.
Повний текст джерелаWan, Wei [Verfasser], Stefan [Akademischer Betreuer] Hecht, and Michael G. [Akademischer Betreuer] Weller. "Molecularly imprinted chromogenic and fluorogenic receptors as optical sensor matrix / Wei Wan. Gutachter: Stefan Hecht ; Michael G. Weller." Berlin : Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2015. http://d-nb.info/1074459423/34.
Повний текст джерелаWagner, Sabine. "Sensory molecularly imprinted polymer (MIP) coatings for nanoparticle- and fiber optic-based assays." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/19808.
Повний текст джерелаFor the detection of these contaminants in low concentration ranges fast and sensitive analytical tools are required. Molecularly imprinted polymers (MIPs) have been used as synthetic materials mimicking molecular recognition by natural receptors due to their ability to recognize selectively a wide range of analytes, their stability and ease of synthesis. They have gained more and more attention in chemical sensing as receptor material for the detection of suitable groups of analytes at low concentrations especially in combination with fluorescence due to the latter’s high sensitivity. This work aimed the development of optical sensor materials using MIPs as recognition elements connected with fluorescence for the sensitive detection of herbicides and antibiotics in water and food samples and their combination with various device formats for the future detection of a wide range of analytes.
Ndangili, Peter Munyao. "Electrochemical and optical modulation of selenide and telluride ternary alloy quantum dots genosensors." Thesis, University of the Western Cape, 2012. http://hdl.handle.net/11394/4025.
Повний текст джерелаElectroanalytical and optical properties of nanoscale materials are very important for biosensing applications as well as for understanding the unique one-dimensional carrier transport mechanism. One-dimensional semiconductor nanomaterials such as semiconductor quantum dots are extremely attractive for designing high-density protein arrays. Because of their high surfaceto-volume ratio, electro-catalytic activity as well as good biocompatibility and novel electron transport properties make them highly attractive materials for ultra-sensitive detection of biological macromolecules via bio-electronic or bio-optic devices. A genosensor or gene based biosensor is an analytical device that employs immobilized deoxyribonucleic acid (DNA) probes as the recognition element and measures specific binding processes such as the formation of deoxyribonucleic acid-deoxyribonucleic acid (DNA-DNA), deoxyribonucleic acid- ribonucleic acid (DNA-RNA) hybrids, or the interactions between proteins or ligand molecules with DNA at the sensor surface.In this thesis, I present four binary and two ternary-electrochemically and optically modulated selenide and telluride quantum dots, all synthesised at room temperature in aqueous media. Cationic gallium (Ga3+) synthesized in form of hydrated gallium perchlorate salt[Ga(ClO4)3.6H2O] from the reaction of hot perchloric acid and gallium metal was used to tailor the optical and electrochemical properties of the selenide and telluride quantum dots. The synthesized cationic gallium also allowed successful synthesis of novel water soluble and biocompatible capped gallium selenide nanocrystals and gallium telluride quantum dots. Cyclic voltammetric studies inferred that presence of gallium in a ZnSe-3MPA quantum dot lattice improved its conductivity and significantly increased the electron transfer rate in ZnTe-3MPA.Utraviolet-visible (UV-vis) studies showed that incorporation of gallium into a ZnSe-3MPA lattice resulted in a blue shift in the absorption edge of ZnSe-3MPA from 350 nm to 325 nm accompanied by decrease in particle size. An amphiphilic bifunctional molecule, 3-Mercaptopropionic acid (3-MPA) was used as a capping agent for all quantum dots. It was found that 3-MPA fully solubilised the quantum dots, made them stable, biocompatible, non agglomerated and improved their electron transfer kinetics when immobilized on gold electrodes.Retention of the capping agent on the quantum dot surface was confirmed by Fourier transform infrared spectroscopy (FTIR) which gave scissor type bending vibrations of C-H groups in the region 1365 cm-1 to 1475 cm-1, stretching vibrations of C=O at 1640 cm-1, symmetric and asymmetric vibrations of the C-H in the region 2850 cm-1 to 3000 cm-1 as well as stretching vibrations of –O-H group at 3435 cm-1. The particle size and level of non-agglomeration of the quantum dots was studied by high resolution transmission electron microscopy (HRTEM). The optical properties of the quantum dots were studied using UV-vis and fluorescence spectroscopic techniques.Quantum dot/nanocrystal modified gold electrodes were prepared by immersing thoroughly cleaned electrodes in the quantum dot/nanocrystal solution, in dark conditions for specific periods of time. The electrochemical properties of the modified electrodes were characterized by cyclic voltammetry (CV), square wave voltammetry (SWV), electrochemical impedance and spectroscopy (EIS). Six sensing platforms were then prepared using quantum dot/nanocrystal, one of which was used for detection of dopamine while the rest were used for detection of a DNA sequence related to 5-enolpyruvylshikimate-3-phosphate synthase, a common vector gene in glyphosate resistant transgenic plants.The first sensing platform, consisting of ZnSe-3MPA modified gold electrode (Au|ZnSe-3MPA) gave rise to a novel method of detecting dopamine in presence of excess uric acid and ascorbic acid. Using a potential window of 0 to 400 mV, the ZnSe-3MPA masked the potential for oxidation of uric and ascorbic acids, allowing detection of dopamine with a detection limit of 2.43 x 10-10 M (for SWV) and 5.65 x 10-10 M (for steady state amperometry), all in presence of excess uric acid (>6500 higher) and ascorbic acid (>16,000 times higher). The detection limit obtained in this sensor was much lower than the concentration of dopamine in human blood(1.31 x 10-9 M), a property that makes this sensor a potential device for detection of levels of dopamine in human blood.The other sensing platforms were prepared by bioconjugation of amine-terminated 20 base oligonucleotide probe DNA (NH2-5′-CCC ACC GGT CCT TCA TGT TC-3′) onto quantum dot modified electrodes with the aid of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). The prepared DNA electrodes were electrostatically hybridized with different sequences which included 5′-GAA CAT GAA GGA CCG GTG GG-3′ (complementary target), 5′-CATAGTTGCAGCTGCCACTG-3′ (non complementary target) and 5′-GATCATGAAGCACCGGAGGG-3′ (3-base mismatched target).The hybridization events were monitored using differential pulse voltammetry (DPV) and SWV by monitoring the guanine oxidation signal or using EIS by monitoring changes in the charge transfer resistance. The quantum dot genosensors were characterized by low detection limits (in the nanomolar range), long linear range (40 - 150 nM) and were able to discriminate among complementary, non-complementary and 3-base mismatched target sequences.
Bonezzi, Paul J. "The development of outer retinal photoresponsivity and the effects of sensory deprivation." University of Akron / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=akron1602525875711526.
Повний текст джерелаIzquierdo, Serra Mercè. "Photoswitchable glutamate receptors to control neurotransmission with light." Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/146130.
Повний текст джерелаL’estudi de la neurotransmissió requereix noves eines moleculars, i els fotocommutadors ofereixen grans possibilitats. Aquesta tesi està centrada en l’ús de receptors de glutamat activables per llum (LiGluRs) pel control de l’activitat neuronal i dels processos de neurosecreció. Al primer bloc de resultats, la permeabilitat a Ca2+ dels receptors de glutamat s’aprofita per manipular de manera directa -independentment del voltatge de membrana- i reversible la concentració intracel•lular de Ca2+ amb llum. Així, és possible desencadenar els processos de secreció en cèl•lules cromafins i de neurotransmissió en neurones hipocampals. A la segona part dels resultats de la tesi, s’investiga l’estimulacio multifotó del receptor de glutamat modificat químicament amb fotocommutadors basats en l’azobenzè. Els resultats mostren l’estimulació per dos fotons del LiGluR, incloent-hi dos fotocommutadors nous, que milloren l’absorció multifotó del commutador azobenzè. Finalment, s’aplica aquesta tècnica per estimular neurones i astròcits amb una resolució a l’escala d’una cèl•lula o de compartiment subcel•lular. Al tercer capítol dels resultats, es descriu un nou mètode per aconseguir el fotocontrol de receptors neuronals endògens, utilitzant lligands covalents. Amb l’aplicació d’aquest mètode es pot fotocommutar l’activitat del receptor de kainat subtipus 1 (GluK1) no mutant, quan se sobreexpressa el receptor en cèl•lules de mamífer. Aquesta estratègia també permet obtenir fotocorrents en cultius de neurones dels ganglis de l’arrel dorsal, on GluK1 és la subunitat de glutamat endògenament més expressada. Els nous mètodes desenvolupats en aquesta tesi milloren la utilització dels LiGluRs, encaminant l’ús dels fotocommutadors cap al control òptic dels receptors endògens, amb la possibilitat d’estimular cèl•lules individuals o estructures subneuronals, fet que situaria els fotocomutados com a eines indispensables per l’estudi del cervell, des de la fisiologia fins als circuits neuronals.
Reaver, Nathan George Frederick. "Development and Characterization of Aptamers for the use in Surface Plasmon Resonance Sensors for the Detection of Glycated Blood Proteins." University of Toledo / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1373319138.
Повний текст джерелаRodrigues, Rúben Miguel Lopes. "Suberin biotech potential: from bactericidal nanoparticles to optical sensors." Doctoral thesis, 2021. http://hdl.handle.net/10362/128745.
Повний текст джерелаKumawat, Nityanand. "Self-referencing Techniques in Optical Label-free Bio-molecular Sensing." Thesis, 2013. https://etd.iisc.ac.in/handle/2005/4577.
Повний текст джерела(9175604), Anna Murray. "Utilizing Amine-Thiol Molecular Precursors for Ag2ZnSnSe4 Thin Films." Thesis, 2020.
Знайти повний текст джерелаThin film photovoltaic materials have garnered much interest recently due to their processability in addition to good properties for conversion of solar photons to usable energy. Amine-thiol chemistry has shown the ability to produce solution processed materials such as Cu2ZnSn(S,Se)4 (CZTSSe), a thin film absorber composed of earth abundant metals. Using similar solution processing methods as those used to produce CZTS, we wish to synthesize a phase pure solution processed material from molecular precursors of metals and metal chalcogenides into an Ag2ZnSnSe4 absorber which lacks the electronic defects that plague CZTSSe. Additionally, we will utilize the reactive dissolution of metal in amine-thiol solution chemistry for a more detailed understanding of how metal-sulfur complexes form and then decompose into films, to gain insight about the conditions that produce stable solutions and high quality films for a better ability to optimize processing conditions.
We find we are able to individually
dissolve zinc metal, tin metal, and silver sulfide precursors to produce
solutions of metal thiolate complexes. Based on results from electrospray
ionization mass spectrometry (ESI-MS), proton nuclear magnetic resonance (1H-NMR),
and extended X-ray absorption fine structure (EXAFS)/ X-ray absorption near
edge spectra (XANES) we propose that these structures contain thiolate
molecules coordinated with Ag, Zn, and Sn in the +1, +2, and +2 oxidation
states respectively. However, mixing these produces an AZTS solution which is
only stable for 3 hours, due to a redox reaction between Ag+ and Sn2+
which forms Sn4+ and insoluble Ag metal. To solve this, we
synthesize SnS2 and show this produces a different Sn-thiolate
complex with fully oxidized Sn4+. This is then used to produce the
first stable AZTS solution, an essential step to fabricating reproducible films.
We use this AZTS solution to fabricate films containing AZTS, and selenize
these films in a tube furnace to produce films which contain AZTSe as well as
secondary phases. We then use rapid thermal processing furnace to remove some
of these secondary phases, and discuss ways to further improve our material
quality.
Shivananju, B. N. "Advanced Multifunctional Bulk Optical & Fiber Bragg Grating Sensing Techniques." Thesis, 2013. https://etd.iisc.ac.in/handle/2005/2611.
Повний текст джерелаShivananju, B. N. "Advanced Multifunctional Bulk Optical & Fiber Bragg Grating Sensing Techniques." Thesis, 2013. http://hdl.handle.net/2005/2611.
Повний текст джерелаΗλιόπουλος, Κωνσταντίνος. "Μελέτη της μη γραμμικής οπτικής απόκρισης φουλλερενικών παραγώγων και νανοσωματιδίων για εφαρμογές σε διατάξεις οπτικών αισθητήρων". Thesis, 2008. http://nemertes.lis.upatras.gr/jspui/handle/10889/1588.
Повний текст джерелаIn this work the third order nonlinear optical response of several photonic materials, has been investigated. These materials were in the form of solutions, colloids or thin films. Initially some basic concepts of nonlinear optics, the physical processes related with it, as well as the physical mechanisms related to the nonlinear refractive index are presented. Then, the nonlinear optical response of Au, Pd and Ag nanoparticles is presented. By using polymers, formation of nanoparticles exhibiting specific sizes can be achieved. Furthermore the polymer does not allow metal aggregation in the system. The nonlinearity of TiO2 films, fullerene derivatives and molecular engines is also investigate. The large response of these systems, combined with the strong dependence on several morphological/structural parameters makes them very promising candidates for several photonic applications.
(11146737), Swapnil Dattatray Deshmukh. "UNVEILING THE AMINE-THIOL MOLECULAR PRECURSOR CHEMISTRY FOR FABRICATION OF SEMICONDUCTING MATERIALS." Thesis, 2021.
Знайти повний текст джерелаMcCleskey, Shawn Catherine 1976. "Optical signaling strategies for use in a multi-component sensor array." 2003. http://hdl.handle.net/2152/12228.
Повний текст джерелаMcCleskey, Shawn Catherine. "Optical signaling strategies for use in a multi-component sensor array." 2003. http://wwwlib.umi.com/cr/utexas/fullcit?p3116387.
Повний текст джерелаLu, Hui-Hsin, and 呂慧歆. "Nonlinear Electrical and Optical Properties of Nano Sensor Array for Stochastic Molecular Interactions." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/75056677446126706940.
Повний текст джерела國立臺灣大學
電機工程學研究所
97
In the biochip applications, the mechanism of specific molecular recognition plays a fundamental role for molecular diagnosis. Traditionally, the dynamics of such an interaction are obtained by spatial and temporal summations of measured signals at macroscopic scale. However, it should be feasible to acquire such information in microscopic environment at single molecule level. The goal of this research is to develop platform technologies for a novel "digital nanoarray", with feature size down to 50 nm, to explore the microscopic measurement of stochastic behaviour at single molecule level and the feasibility studies of acquiring molecular dynamics by spatial-temporal summations of non-labelling biomolecules. These platform technologies include (1) nanofabrication of zero dimensional (0 D) nanodot array for developing a biomimicking platform and one-dimensional (1 D) nanowire array of conducting polymer for NO gas sensing; (2) a multi-functional dark-field microscopy (DFM) with nano-resolution down to 40 nm and spectroscopic function for characterizing observed nanostructure; and (3) a theoretical model for simulation and analysis of one and two dimensional of stochastic molecular interaction. First of all, we have used dip-pen nanotechnology (DPN) to fabricate nanoarray and nanowires for the studies of their nonlinear optical and electrical properties at large scale. With the fabricated nanodot array of 50 nm in diameter, we were able to immobilize bio-molecules on to prepared surface and acquire both 1 D and 2 D signals for further calculation of the spatial-temporal summations. The non-labeling detection of biomolecular recognition has been done by scanning images of AFM, and the resultant height of streptavidin is about 4 nm, which is close to the theoretical value. To improve the quality of surface preparation, we have used the atomic layer deposition (ALD) to deposit a 2 nm thickness of alumina oxide as a passivation layer to minimize non-specific binding to the native glass surface. We have compared the performance of both oxide surface of germanium and silicon substrate with the bare silicon surface. In the prepared 1 Dnanowire of both 68 nm and 300nm in line width, we are able to precisely align the nanowires across the gap of two microelectrodes. The nonlinear I-V curves of single nanowire were measured and analyzed at room temperature for practical applications. Several parallel and series circuitries of nanowire are measured for the verification of theoretical calculation. Finally, the nanowire array is applied for NO detection. In order to observe the stochastic behavior of single molecule on the digital nanoarray, it is essential to develop an optical microscopy beyond the diffraction limits. Herein, we reported a multifunctional optical microscopic system that combined the features of dark-field microscopy (DFM) with a spectrophotometric function to characterize the nanoarray for both qualitative and quantitative analysis. We have used various formats of optical storage discs, which have different feature sizes in the pitch of track patterns, to demonstrate the resolving power of this system. The calculated resolution is down to 40 nm. Moreover, a thin gold layer is deposited on the disc to study the optical enhancement of pit features and the change of absorption peaks due to surface Plasmon resonance (SPR) effect. A significant change of absorption peak is obtained by depositing a thin film of biolmolecules on the gold film. These results indicate the possible use of this nanoarray platform on medical diagnosis. The stochastic model of behavior of biomolecular interactions is similar to the bi-state (on-off) of channel proteins. Our simulation results show that 100 of molecular interactions are the same in both 1 D and 2 D signals. After this simulation process, we are able to determine the proper size of nanoarray by evaluating the reasonable errors of possibility. We have used deposited 1 D data from internet for our own purposes. This model can be applied to the multi-state systems in the future.
岩花, 宗一郎, and Soichiro Iwahana. "Studies on Synthesis and Structures of Optically Active Riboflavin-Derived Molecules and Polymers and Their Application as Organocatalysts and Sensory Materials." Thesis, 2014. http://hdl.handle.net/2237/20304.
Повний текст джерела