Дисертації з теми "Optical Biosensing"
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King, Branden Joel. "Tapered Optical Fiber Platform for Biosensing Applications." University of Dayton / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1398708775.
Повний текст джерелаBlyth, David John. "Optical biosensing using sol-gel technology." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338063.
Повний текст джерелаGreenhalgh, Andrew Bryce. "Tapered polymer optical fibres for biosensing." Thesis, Manchester Metropolitan University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423074.
Повний текст джерелаD'Imperio, Luke A. "Biosensing-inspired Nanostructures:." Thesis, Boston College, 2019. http://hdl.handle.net/2345/bc-ir:108627.
Повний текст джерелаNanoscale biosensing devices improve and enable detection mechanisms by taking advantage of properties inherent to nanoscale structures. This thesis primarily describes the development, characterization and application of two such nanoscale structures. Namely, these two biosensing devices discussed herein are (1) an extended-core coaxial nanogap electrode array, the ‘ECC’ and (2) a plasmonic resonance optical filter array, the ‘plasmonic halo’. For the former project, I discuss the materials and processing considerations that were involved in the making of the ECC device, including the nanoscale fabrication, experimental apparatuses, and the chemical and biological materials involved. I summarize the ECC sensitivity that was superior to those of conventional detection methods and proof-of-concept bio-functionalization of the sensing device. For the latter project, I discuss the path of designing a biosensing device based on the plasmonic properties observed in the plasmonic halo, including the plasmonic structures, materials, fabrication, experimental equipment, and the biological materials and protocols
Thesis (PhD) — Boston College, 2019
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Physics
Jamali, Abdul Aleem [Verfasser]. "Optical Antennas for Biosensing Applications / Abdul Aleem Jamali." Kassel : Universitätsbibliothek Kassel, 2015. http://d-nb.info/1073856577/34.
Повний текст джерелаKrasovska, Inese. "Optical Properties of Silicon Nanopillar Arrays for Biosensing." Thesis, KTH, Skolan för informations- och kommunikationsteknik (ICT), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-175760.
Повний текст джерелаChung, Chun Lam Cathy K. S. "Optical biosensing of iron(III) in oceanic waters." Thesis, University of East Anglia, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405393.
Повний текст джерелаBarreau, Stephanie. "Biosensing with sol-gel-immobilised proteins." Thesis, Loughborough University, 1999. https://dspace.lboro.ac.uk/2134/27275.
Повний текст джерелаOleksiy, Krupin. "Biosensing Using Long-Range Surface Plasmon-Polariton Waveguides." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34210.
Повний текст джерелаNemzer, Louis R. "Oxidoreductase Immobilization in Reprecipitated Polyaniline Nanostructures for Optical Biosensing Applications." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1265751296.
Повний текст джерелаBertó, Roselló Francisco. "Numerical modelling of nanoporous anodic alumina photonic structures for optical biosensing." Doctoral thesis, Universitat Rovira i Virgili, 2018. http://hdl.handle.net/10803/665620.
Повний текст джерелаAlgunos materiales muestran propiedades físicas, ópticas y químicas particulares a nanoescala. La nanotecnología permite al científico mejorar sus propiedades modificando la estructura de la materia en este nivel y aprovecharlas para el desarrollo de nuevos dispositivos y aplicaciones. Particularmente importante es la investigación en biosensores con el fin de obtener dispositivos más sensibles y confiables para desplegarlos en la sociedad en forma de sistemas de diagnóstico rentables y confiables. La fabricación de tales dispositivos en algunos casos implica la manipulación de la luz en estos materiales en la nanoescala, para lo cual, es necesario tener un conocimiento profundo del comportamiento óptico de la estructura. En este sentido, la elección del material apropiado es decisiva en el desarrollo de dispositivos biosensores. La alúmina anódica nanoporosa (AAN) es un material poroso autoordenado cuyas propiedades ópticas en lo visible, su gran adaptabilidad de la estructura, su capacidad para actuar como soporte o andamio de objetos pequeños y su estabilidad en condiciones biológicas lo hacen especialmente apropiado como plataforma para el desarrollo de biosensores ópticos. Los estudios teóricos sobre las propiedades ópticas de la AAN son un campo fundamental de investigación. El modelado numérico de estas propiedades permite comprender su relación con las características estructurales de la AAN, proporcionando un marco conceptual para el análisis de su comportamiento óptico. En esta tesis, hemos analizado la idoneidad y desarrollado varios modelos predictivos para las propiedades ópticas de la AAN válidos en una amplia gama de características geométricas. Posteriormente, nuestro modelo de simulación se aplicó para estudiar la idoneidad de dos tipos de estructuras AAN revestidas de oro como plataforma para el desarrollo de biosensores plasmónicos por reflectometría. Finalmente, se ha realizado un estudio teórico del comportamiento óptico mediante modelado numérico de estructuras basadas en AAN con un gradiente en el índice de refracción.
Some materials shown particular physical, optical and chemical properties at the nanoscale. The nanotechnology permits to scientist enhance their properties modifying the matter structure at this level and take advantage of them for the development of new devices and applications. Particularly important is the research in biosensing in order to obtain more sensitive and reliable devices with a view for their deployment to society in the form of cost-effective and reliable diagnostic systems. The manufacturing of such devices in some cases implies the manipulation of the light in these materials at the nanoscale, for which it implies to have a deep knowledge of the optical behaviour of the structure. In this sense, the election of the appropriate material is decisive in the development of biosensing devices. Nanoporous anodic alumina (NAA) is a self-ordered porous material which their optical properties in the visible, their great tunability of the structure, their ability to act as a holder or scaffold of small objects and their stability under biological conditions make it especially appropriate as a platform for the development of optical biosensors. Theoretical studies on the NAA optical properties is a fundamental field of research. The numerical modelling of these properties permits to understand their relationship with the structural features of the NAA, providing a conceptual framework for the analysis of their optical behaviour. In this thesis, we have analyzed the suitability and developed several predictive models for the optical properties of the NAA valid in a wide range of geometrical characteristics. Subsequently our simulation model has been applied to study the suitability of two types of gold-coated NAA structures as a platform for reflectometric-based plasmonic biosensors. Finally, a theoretical study of the optical behaviour by means of numerical modelling of gradient-index NAA (NAA-GI) based structures has been performed.
Martínez, Pérez Paula. "Development and Optimization of Experimental Biosensing Protocols Using Porous Optical Transducers." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/172541.
Повний текст джерела[CA] Els biosensors són dispositius analítics amb aplicabilitat en diferents camps i amb nombrosos avantatges enfront d'altres mètodes analítics convencionals, com són l'ús de xicotets volums de mostra i reactius, la seua sensibilitat i la seua ràpida resposta, sense necessitat de pretractament de la mostra, equips cars o personal especialitzat. No obstant això, es tracta d'un camp d'investigació relativament nou en el qual encara queda molt camí per fer. Aquesta Tesi doctoral pretén aportar el seu òbol a aquest camp de coneixement mitjançant l'estudi del potencial de diferents materials porosos com a transductors per al desenvolupament de biosensors òptics amb resposta en temps real i sense marcatges. Els materials proposats van des d'aquells artificialment sintetitzats, com a silici porós (SiP), nanofibras (NFs) polimèriques o membranes polimèriques comercials, fins a materials naturals amb propietats fotòniques que encara no havien sigut explotades per al sensat, com són els exoesquelets de biosílice de diatomees. Tots ells tenen en comú la simplicitat en la seua obtenció, evitant costosos i laboriosos processos de nanofabricació. Per al seu estudi, s'analitzarà la seua resposta òptica i, en aquells casos en els quals aquesta permeta dur a terme experiments de detecció, es desenvoluparan estratègies per a la seua biofuncionalizació i la seua implementació en experiments de biosensat. En el cas del SiP i les NFs s'han optimitzat els paràmetres de fabricació per a obtenir una resposta òptica adequada que permeta la seua interrogació. A continuació, s'ha dut a terme la seua biofuncionalizació emprant mètodes covalents i no covalents, així com diferents bioreceptors (aptàmers d'ADN i anticossos) per a estudiar el seu potencial i les seues limitacions com a biosensors. En el cas de les membranes comercials i l'exoesquelet de sílice de diatomees, s'ha caracteritzat la seua resposta òptica i s'han dut a terme experiments de sensat d'índex de refracció per a estudiar la seua sensibilitat. Així mateix, s'ha desenvolupat un mètode de funcionalizació de la superfície de l'exoesquelet de diatomees basat en l'ús de polielectròlits catiònics. Com a resultat, s'ha demostrat el potencial tant de NFs per al desenvolupament de biosensors, com el de membranes comercials per a sensors amb una aplicació que no requerisca una elevada sensibilitat però sí un baix cost. A més, s'ha posat de manifest el gran potencial de l'exoesquelet de diatomees per al desenvolupament de sensors basats en la seua resposta òptica. Per contra, les limitacions trobades en el desenvolupament de biosensors basats en SiP han evidenciat la necessitat d'un estudi rigorós i l'optimització de l'estructura dels materials porosos prèviament a ser usats en (bio)sensat.
[EN] Biosensors are analytical devices with application in diverse fields and with several advantages relative to other conventional methods, such as the use of small volumes of sample and reagents, their sensitivity and their fast response, without the need of the sample pretreatment, expensive equipments or specialised technicians. Nevertheless, this is a relatively new research field in which there is a long way to go yet. This doctoral Thesis aims at doing its bit to this field of knowledge by studying the potential of different porous materials as transducers for the development of real-time and label-free optical biosensors. The proposed materials range from those artificially synthesised, such as porous silicon (pSi), polymeric nanofibres (NFs) or commercial polymeric membranes, to natural materials with photonic properties that had not been exploited for sensing yet, such as biosilica exoskeletons of diatoms. All of them have in common its simple production, avoiding expensive and laborious nanofabrication processes. For their study, their optical response will be analysed and, in those cases in which such optical response allows performing detection experiments, strategies for their biofunctionalisation and their implementation in biosensing experiments will be developed as well. Regarding pSi and NFs, the fabrication parameters were optimised to get a suitable optical response for their interrogation. Afterwards, their surface functionalisation was carried out by covalent and non-covalent methods, as well as different bioreceptors (DNA aptamers and antibodies), to study their potential and their constraints as biosensors. Concerning commercial membranes and the biosilica exoskeleton of diatoms, their optical response was characterised and refractive index sensing experiments were carried out to study their sensitivity. Additionally, a biofunctionalisation method for the surface of the diatoms exoskeleton was developed based on the use of cationic polyelectrolytes. As a result, it was demonstrated the potential of NFs for the development of biosensors, as well as the potential of commercial membranes for developing sensors for an application that does not require a high sensitivity but a low cost. Furthermore, the great potential of biosilica exoskeleton of diatoms for the development of sensors based on their optical response has been revealed. By contrast, the constraints found in the development of pSi illustrate the importance of an accurate study and optimisation of porous materials structure before using them for (bio)sensing.
Martínez Pérez, P. (2021). Development and Optimization of Experimental Biosensing Protocols Using Porous Optical Transducers [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/172541
TESIS
Badmos, Abdulyezir. "Advanced optical fibre gratings for nano-structural characterisation and biosensing applications." Thesis, Aston University, 2017. http://publications.aston.ac.uk/30869/.
Повний текст джерелаYavas, Ozlem. "On-chip biosensing platforms based on gold and silicon optical nano-resonators." Doctoral thesis, Universitat Politècnica de Catalunya, 2019. http://hdl.handle.net/10803/667307.
Повний текст джерелаLa instrumentación Point-of-care (POC) es compacta, móvil y permite una detección rápida, razón por la que se prevé que sean de gran ayuda en áreas como el diagnostico precoz, la monitorización de tratamientos y la medicina personalizada, revolucionando los modelos sanitarios, especialmente en las zonas de difícil acceso y con menos recursos. La necesidad de este tipo de dispositivos impulsa el desarrollo de novedosas tecnologías en el campo de los bio-sensores. Diseñar equipos para la detección de bio-moléculas que sean rápidos, pequeños y sencillos es un reto que requiere la integración de múltiples campos de la ciencia y la ingeniería. Los nano-resonadores ópticos muestran un gran potencial como bio-sensores sin necesidad de marcaje, gracias a su capacidad de acoplase directamente con la luz en modos menores que la longitud de onda. Los nano-resonadores metálicos basados en resonancias plasmónicas superficiales localizadas (LSPR) han sido estudiados y han demostrado ser una firme alternativa a los ya comerciales basados en resonancias plasmónicas superficiales (SPR). Los nano-resonadores dieléctricos han sido recientemente objeto de atención debido a sus bajas perdidas y la capacidad de manipular los componentes eléctricos y magnéticos de la luz. En esta tesis presentamos avances en el campo de la bio-detección y en el uso de los nano-resonadores ópticos como potenciales herramientas para la detección de enfermedades y monitorización de los tratamientos. Hemos desarrollado y evaluado distintas plataformas de detección combinando los nano-resonadores ópticos, tanto metálicos como dieléctricos, con las más avanzadas técnicas de microfluídica y química de superficies. En primer lugar, nos centramos en el desarrollo de un dispositivo microfluídico basado en sensores LSPR de oro que permite multiplexar 32 canales. Los 32 sensores se monitorizan en tiempo real para demostrar la cuantificación de 4 marcadores de cáncer de mama en suero sanguíneo humano. Demostramos que mediante la optimización de los ensayos se pueden alcanzar bajos límites de detección (LOD), lo que allana el camino hacia dispositivos POC de uso clínico. Por otro lado, hemos utilizado los nano-resonadores de silicio integrados con la microfluídica para también detectar marcadores de cáncer en suero. Estos sensores, cuyo principio de funcionamiento se basa en resonancias de MIE, han demostrado ser una alternativa razonable a los sensores de oro. Además, demostramos que un proceso de fabricación de nano-resonadores de silicio rápido, escalable y de bajo coste da lugar a límites de detección suficientes para la producción de futuras POC. También realizamos un minucioso estudio del rol de las resonancias eléctricas y magnéticas en dichos sensores y su relación con el desplazamiento y el cambio magnitud de la resonancia del sensor global. Nuestro trabajo es un avance en el desarrollo de futuros instrumentos POC rápidos y baratos en el ámbito de la salud a escala molecular.
Bailey, J. "Multiscale optical patterning : using micro and nano periodic structures to create novel optical devices with applications to biosensing." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1519804/.
Повний текст джерелаMontali, Laura <1994>. "Smartphone-based analytical devices with optical detection for on-site biosensing: environmental, food and forensic applications." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2022. http://amsdottorato.unibo.it/10021/1/TESI_LAURA_MONTALI_SUBMISSION_20_01_22.pdf.
Повний текст джерелаWang, M. (Meng). "Polymer integrated Young interferometers for label-free biosensing applications." Doctoral thesis, Oulun yliopisto, 2012. http://urn.fi/urn:isbn:9789514299643.
Повний текст джерелаTiivistelmä Integroidulla optiikalla toteutetut anturit mahdollistavat biomolekulaarisen vuorovaikutuksen tutkimisen käyttäen herkkiä moniparametrisia ja merkkiaineettomia menetelmiä. Näiden bioantureiden valmistukseen käytetään tavallisesti CMOS-teknologian piiristä tuttuja epäorgaanisia puolijohteita ja eristemateriaaleja. Viime aikoina on kuitenkin polymeeristen materiaalien käyttöä integroidussa optiikassa tutkittu merkittävästi johtuen polymeerien hyvistä optisista ominaisuuksista, monipuolisesta työstettävyydestä ja edullisista kustannuksista. Tämän työn tarkoituksena on kehittää edullisia, kertakäyttöisiä, pääasiallisesti polymeerisistä materiaaleista valmistettuja bioantureita, jotka vastaavat suorituskyvyltään epäorgaanisista materiaaleista valmistettuja integroidun optiikan antureita. Tässä työssä kehitetyt polymeeriset integroidun optiikan bioanturit perustuvat Youngin interferometriin mahdollistaen ympäristökohinan kompensoinnin ja ne tuottavat pintavuorovaikutusten tutkimiseen jaksoittaisen interferenssikuvion. Työssä hyödynnettiin kolmea erilaista valokanavarakennetta huomioiden niiden käytön helppous, valmistuksen yksinkertaisuus ja mittausherkkyys. Yksi kehitetyistä polymeerisistä bioantureista koostui päällekkäisistä kerrostetuista polymeerikerroksista. Toisen tutkitun rakenteen toiminta puolestaan perustui käänteiseen harjannevalokanavaan. Mittausherkkyyttä parannettiin pinnoittamalla polymeerirakenne Ta2O5-pinnoitteella. Näin muodostui kerrostettu komposiittivalokanava, joka oli tässä työssä tutkittu kolmas sensorirakenne. Itse bioanturien lisäksi kehitettiin myös valmistusprosessi, jossa hyödynnettiin UV-painatusta ja nestefaasipinnoitusta. Tässä työssä havaittiin lisäksi, että kehitetty yksinkertainen valmistusmenetelmä on paitsi toimiva, myös mahdollisesti siirrettävissä rullalta rullalle valmistus- ja tuotantoteknologiaan. Kehitettyjen anturien kalibrointi suoritettiin homogeenisella taitekerroinmittauksella käyttäen liuoksia, jotka valmistettiin glukoosista ja deionisoidusta vedestä. Kehitettyjen anturien soveltuvuus spesifien molekyylien tunnistamista varten todennettiin tutkimalla vasta-aineiden ja antigeenien sitoutumisreaktioita ja vuorovaikutusta C-reaktiivisella proteiinilla ja sen konjugaateilla. Lisäksi työssä tutkittiin veden absorption vaikutusta mittauksen stabiilisuuteen. Tutkimuksessa suoritettiin vertailu kehitettyjen anturien ja niiden ominaisuuksien välillä kiinnittäen huomiota mittausherkkyyteen, stabiilisuuteen, määritys- ja toteamisrajoihin ja muihin anturien valmistukseen sekä käyttöön liittyviin keskeisiin piirteisiin. Tulokset osoittavat, että Ta2O5-pinnoitetun polymeerivalokanavan mittausherkkyys oli suurin vertailluista rakenteista. Homogeenisessä taitekerroinmittauksessa saavutettu määritys- ja toteamisraja oli 9×10-7 taitekerroinyksikköä (RIU). Pintamassatiheysmittauksessa saavuttu tulos oli 270 fg/mm2
Danilov, Artem. "Design, characterisation and biosensing applications of nanoperiodic plasmonic metamaterials." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0110/document.
Повний текст джерелаThis thesis consideres novel promissing architechtures of plasmonic metamaterial for biosensing, including: (I) 2D periodic arrays of Au nanoparticles, which can support diffractively coupled surface lattice resonances; (II) 3D periodic arrays based on woodpile-assembly plasmonic crystals, which can support novel delocalized plasmonic modes over 3D structure. A systematic study of conditions of plasmon excitation, properties and sensitivity to local environment is presented. It is shown that such arrays can combine very high spectral sensitivity (400nm/RIU and 2600 nm/RIU, respectively) and exceptionally high phase sensitivity (> 105 deg./RIU) and can be used for the improvement of current state-of-the-art biosensing technology. Finally, a method for probing electric field excited by plasmonic nanostructures (single nanoparticles, dimers) is proposed. It is implied that this method will help to design structures for SERS, which will later be used as an additional informational channel for biosensing
Romero-García, Sebastián [Verfasser], Jeremy [Akademischer Betreuer] Witzens, and Iñigo [Akademischer Betreuer] Molina-Fernández. "Integrated photonics for high speed optical interconnects and biosensing applications / Sebastián Romero-García ; Jeremy Witzens, Iñigo Molina-Fernández." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/113040305X/34.
Повний текст джерелаNicolini, Ariana Marie, and Ariana Marie Nicolini. "Single-Step, Optical Biosensors for the Rapid and Sensitive Detection of Bacterial and Viral Pathogens." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/623158.
Повний текст джерелаDoherty, Brenda [Verfasser], Markus A. [Gutachter] Schmidt, Rachel [Gutachter] Grange, and Isabelle Philippa [Gutachter] Staude. "Plasmonic microstructured optical fibres : an efficient platform towards biosensing / Brenda Doherty ; Gutachter: Markus A. Schmidt, Rachel Grange, Isabelle Philippa Staude." Jena : Friedrich-Schiller-Universität Jena, 2020. http://d-nb.info/121099853X/34.
Повний текст джерелаRye, Jan-Michael. "Spatial Modulation Spectroscopy Of Single Nano-Objects In A Liquid Environment For Biosensing Applications." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1053/document.
Повний текст джерелаAdvances in the development of rapid, accurate and highly sensitive methods for detecting target analytes in solution will provide crucial tools for research and applications in medicine and molecular biology. One of the currently most promising approaches is the development of nanosensors based on the localized surface plasmon resonance (LSPR) of noble metal nano-objects (MNOs), which is an optical response that depends on their size, shape, composition and local environment. The ability to measure the modification of the reponse of a single MNO in the presence of a target analyte would allow each object to act as an independent probe with increased sensitivity as the signal would be isolated from the averaging effects of ensemble measurements. Furthermore it would allow the development of micrometric, functionalized multiprobe samples for multitarget label-free assays.In this work, a novel experimental setup based on the spatial modulation spectroscopy (SMS) technique has been developed to measure the optical response of individual nano-objects in a liquid environment. In parallel, a new technique has also been developed to elaborate stable probes for measurements with the new setup, with a focus on gold bipyramids due to numerous qualities that make them excellent candidates for biosensing probes. The setup has been used to measure the response of individual objects in environments of different real refractive indices and the detected changes have been shown to be in good agreement with theoretical calculations. Numerical studies have also been performed to investigate the influence on the optical response of numerous factors encountered in the studied systems
Gylfason, Kristinn Björgvin. "Integrated Optical Slot-Waveguide Ring Resonator Sensor Arrays for Lab-on-Chip Applications." Doctoral thesis, KTH, Mikrosystemteknik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-12214.
Повний текст джерелаQc20100715
SABIO
Nagarajan, Vivek Krishna. "Fluorescence Assisted Portable Cell Counting System." University of Akron / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1376577419.
Повний текст джерелаCaroselli, Raffaele. "Development of high sensitivity photonic sensing structures based on porous silicon substrates." Doctoral thesis, Universitat Politècnica de València, 2018. http://hdl.handle.net/10251/107318.
Повний текст джерелаHealth and well-being have always been the center of attention of many research institutions and companies around the world. This led the technology to develop in the chemical, biological, medical and clinical fields with the aim to provide a better protection to the human being. As a consequence, a competition is born between the time necessary to the disease to progress and the time necessary to man to treat such disease. In order to win this competition, it is necessary to act with anticipation, when disease is not too developed yet. This is possible by performing an early-detection. The achievement of this goal paves the way for the development of optical biosensing devices able to detect the presence of certain molecules at extremely low concentrations. Among them, photonic integrated structures are finding a great success due to their considerably high sensitivity. However, the sensing mechanism of these structures is based on the interaction between the evanescent wave, propagating along the structure surface, and the target analyte to detect. In this way, not all the field propagating in the photonic structure is used for sensing purposes, but rather only a small amount of it. This represents a crucial limitation of the integrated photonics based sensors. The aim of this PhD Thesis is to overcome this limitation and to develop more sensitive photonic sensing structures able to detect the lowest concentration possible. To this aim, we focused on the study of porous silicon as platform for the development of optical structures with extremely high sensitivities thanks to the fact that the sensing interaction takes place directly inside the structure itself, allowing to exploit all the field propagating in the structure.
La salut i el benestar sempre han sigut el centre d'atenció de moltes institucions de recerca i empreses de tot el món. Açò va portar a la tecnologia a desenvolupar-se en els camps químic, biològic, mèdic i clínic amb l'objectiu de proporcionar una millor protecció a l'ésser humà. Com a conseqüència, ha sorgit una competició entre el temps necessari per que la malaltia progresse i el temps necessari per que l'home tracte aquesta malaltia. Per a guanyar aquesta competició, és necessari actuar amb anticipació, quan la malaltia encara no està massa desenvolupada. Açò és possible realitzant una detecció precoç de la malaltia. L'assoliment d'aquest objectiu facilita el camí per al desenvolupament de dispositius òptics de biosensat capaços de detectar la presència de certes molècules en concentracions extremadament baixes. Entre ells, les estructures fotòniques integrades estan tenint un gran èxit a causa de la seua considerablement alta sensibilitat. No obstant açò, el mecanisme de detecció d'aquestes estructures es basa en la interacció entre l'ona evanescent, que es propaga al llarg de la superfície de l'estructura, i l'analit a detectar. D'aquesta forma, no tot el camp que es propaga en l'estructura fotònica s'usa amb finalitats de detecció, sinó solament una xicoteta quantitat d'aquest. Açò representa una limitació crucial dels sensors basats en fotònica integrada. L'objectiu d'aquesta tesi doctoral és superar aquesta limitació i desenvolupar estructures fotòniques de sensat més sensibles que siguen capaces de detectar les concentracions més baixes possibles. Amb aquest objectiu, ens centrem en l'estudi del silici porós com a plataforma per al desenvolupament d'estructures òptiques amb sensibilitats extremadament altes gràcies a que la interacció de sensat es realitza directament dins de la pròpia estructura, el que permet explotar tot el camp que es propaga.
Caroselli, R. (2018). Development of high sensitivity photonic sensing structures based on porous silicon substrates [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/107318
TESIS
Beamer, Diane Krupp. "MINIMALLY INVASIVE OPTICAL SENSING OF GOLD AND SILVER NANOPARTICLE AGGREGATION:A PRELIMINARY INVESTIGATION." Miami University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1376405013.
Повний текст джерелаPaturi, Naveen Kumar. "Analysis of photonic crystal defects for biosensing applications." Morgantown, W. Va. : [West Virginia University Libraries], 2006. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4861.
Повний текст джерелаTitle from document title page. Document formatted into pages; contains viii, 70 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 55-57).
Holmström, Nils Brage. "Optimal pacing with an implantable pO₂ sensor /." Stockholm : Tekniska högsk, 1999. http://www.lib.kth.se/abs99/holm0917.pdf.
Повний текст джерелаLoebel, Nicolas G. "Singlemode fiber interferometric biosensors /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/8026.
Повний текст джерелаPal, Chandana. "Thin films of non-peripherally substituted liquid crystalline phthalocyanines A." Thesis, Brunel University, 2014. http://bura.brunel.ac.uk/handle/2438/8997.
Повний текст джерелаKarim, Farzia. "Active and Ultrasensitive Chemical and Biosensing through Optothermally Generated Microbubble." University of Dayton / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1591699029953022.
Повний текст джерелаZhao, Xin. "Study of Multimode Extrinsic Fabry-Perot Interferometric Fiber Optic Sensor on Biosensing." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/34534.
Повний текст джерела
In this thesis, a multimode fiber-optic sensor has been developed based on extrinsic Fabry-Perot interferometry (EFPI) for the measurement of optical thickness in self-assembled thin film layers as well as for the immunosensing test. The sensor was fabricated by connecting a multimode fiber (MMF) and a silica wafer. A Fabry-Perot cavity was formed by the reflections from the two interfaces of the wafer. The negatively charged silica wafer could be used as the substrate for the thin film immobilization scheme. The sensor is incorporated into the white-light interferometric system. By monitoring the optical cavity length increment, the self-assembled thin film thickness was measured; the immunoreaction between immunoglobulin G (IgG) and anti-IgG was investigated.
Master of Science
Joel, Smita. "ENGINEERING PROTEINS WITH UNIQUE CHARACTERISTICS FOR DIAGNOSTICS AND BIOSENSORS." UKnowledge, 2011. http://uknowledge.uky.edu/gradschool_diss/180.
Повний текст джерелаDe, Bonnault Sandie. "Conception, fabrication et caractérisation d'un biocapteur SPR à base de guides d'ondes photoniques sur substrat de verre." Thèse, Université de Sherbrooke, 2016. http://hdl.handle.net/11143/8957.
Повний текст джерелаAbstract : In spite of the growing number of available biosensors, many biochemical reactions and biological components have not yet been studied in detail. Among them, some require the combination of several detection techniques in order to retrieve enough information to characterize them fully. An unknown reaction based, for example, on DNA hybridization could be characterized with an electrochemical sensor, a mechanical sensor and an optical sensor, each giving a different type of information. The main objective of the work presented here is to design, fabricate and characterize a flexible integrated optical biosensor based on surface plasmon resonance, intended to be then combined with other detection techniques, and in particular, a microcalorimeter. Surface Plasmon Resonance (SPR) is well known to be a sensitive technique for surface-based biochemical detection. It has the advantage to be an unlabeled method and provides real time information on the kinetics of a reaction. The flexibility of the proposed SPR biosensor comes from the fact that it is designed for a large range of analyte refractive indices, from 1.33 to 1.48. These values are suitable for most biological entities and their ligand layers, and especially for hydrophilic polymer matrices used to trap DNA or protein entities and introduced in this work. As several biochemical studies require the simultaneous comparison of measurements to a reference or to another measurement, the second objective of this project is to study the potential of multi-analyte detection in an integrated SPR device on glass. The first three chapters of the thesis are focused on the main objective. The design based on two different models is presented, at the same time as the related simulation tools. The first model is based on the weak coupling approximation and permits to obtain most of the information for the device’s design. The second model, having no approximation, is used to validate the first model and complete and refine the design. The fabrication process of the glass chip is then introduced, as well as the characterization instruments and protocols. A device is obtained, with a volumetric sensitivity between 1000 nm/RIU and 6000 nm/RIU depending on the analyte refractive index. The 3D integration of the waveguide within the glass substrate makes the device extremely compact and adapted to the integration with the microcalorimeter in particular. The last chapter describes the study of several spectral multiplexing techniques adapted to an integrated SPR system using the glass technology. The goal is to provide at least two simultaneous measurements. Several detection techniques are examined and the related devices are designed, fabricated and characterized.
Bonnault, Sandie de. "Conception, fabrication et caractérisation d'un biocapteur SPR à base de guides d'ondes photoniques sur substrat de verre." Thesis, Université Grenoble Alpes (ComUE), 2016. http://www.theses.fr/2016GREAT040/document.
Повний текст джерелаIn spite of the growing number of available biosensors, many biochemical reactions and biological components have not yet been studied in detail. Among them, some require the combination of several detection techniques in order to retrieve enough information to characterize them fully. An unknown reaction based, for example, on DNA hybridization could be characterized with an electrochemical sensor, a mechanical sensor and an optical sensor, each giving a different type of information.MuThe main objective of the work presented here is to design, fabricate and characterize a flexible integrated optical biosensor based on surface plasmon resonance, intended to be then combined with other detection techniques. Surface Plasmon Resonance (SPR) is well known to be a sensitive technique for surface-based biochemical detection. It has the advantage to be an unlabeled method and provides real time information on the kinetics of a reaction. The use of an integrated technology enables us to integrate several sensors on the same chip for the same sample, making them compact and low-cost. The flexibility of the proposed SPR biosensor comes from the fact that it is designed for a large range of analyte refractive indices, from 1.33 to 1.48 in the 600 nm-1000 nm wavelength range. These values are suitable for most biological entities and their ligand layers, and especially for hydrophilic polymer matrices used to trap DNA or protein entities. These biochemical matrices are used more and more for their ability to trap high densities of analyte, provide a strong binding and serve as an active detection medium with good anti-fouling properties.MuAs several biochemical studies require the simultaneous comparison of measurements to a reference or to another measurement, the second objective of this project is to study the potential of multianalyte detection in an integrated SPR device on glass.The first three chapters of the thesis are focused on the main objective. The design according to predefined specifications is presented, at the same time as the simulation tools. The fabrication process of the glass chip is introduced, as well as the characterization instruments and protocols. Simulation and experimental results are then compared, and the device performance is assessed.The last chapter describes the study of several spectral multiplexing techniques adapted to an integrated SPR system using the glass technology. The goal is to provide at least two simultaneous measurements. Several detection techniques are examined and the related devices are designed, fabricated and characterized
Sendowski, Jacob Benjamin. "On-Chip Integrated Label-Free Optical Biosensing." Thesis, 2013. https://thesis.library.caltech.edu/7812/1/JS_Thesis.pdf.
Повний текст джерелаThis thesis investigates the design and implementation of a label-free optical biosensing system utilizing a robust on-chip integrated platform. The goal has been to transition optical micro-resonator based label-free biosensing from a laborious and delicate laboratory demonstration to a tool for the analytical life scientist. This has been pursued along four avenues: (1) the design and fabrication of high-$Q$ integrated planar microdisk optical resonators in silicon nitride on silica, (2) the demonstration of a high speed optoelectronic swept frequency laser source, (3) the development and integration of a microfluidic analyte delivery system, and (4) the introduction of a novel differential measurement technique for the reduction of environmental noise.
The optical part of this system combines the results of two major recent developments in the field of optical and laser physics: the high-$Q$ optical resonator and the phase-locked electronically controlled swept-frequency semiconductor laser. The laser operates at a wavelength relevant for aqueous sensing, and replaces expensive and fragile mechanically-tuned laser sources whose frequency sweeps have limited speed, accuracy and reliability. The high-$Q$ optical resonator is part of a monolithic unit with an integrated optical waveguide, and is fabricated using standard semiconductor lithography methods. Monolithic integration makes the system significantly more robust and flexible compared to current, fragile embodiments that rely on the precarious coupling of fragile optical fibers to resonators. The silicon nitride on silica material system allows for future manifestations at shorter wavelengths. The sensor also includes an integrated microfluidic flow cell for precise and low volume delivery of analytes to the resonator surface. We demonstrate the refractive index sensing action of the system as well as the specific and nonspecific adsorption of proteins onto the resonator surface with high sensitivity. Measurement challenges due to environmental noise that hamper system performance are discussed and a differential sensing measurement is proposed, implemented, and demonstrated resulting in the restoration of a high performance sensing measurement.
The instrument developed in this work represents an adaptable and cost-effective platform capable of various sensitive, label-free measurements relevant to the study of biophysics, biomolecular interactions, cell signaling, and a wide range of other life science fields. Further development is necessary for it to be capable of binding assays, or thermodynamic and kinetics measurements; however, this work has laid the foundation for the demonstration of these applications.
Chen, Wei. "Synthesis of Optical Nanodevices with Application in Biosensing." Thesis, 2010. http://spectrum.library.concordia.ca/7455/1/Chen_MASc_s2011.pdf.
Повний текст джерелаAhl, Stefanie Elisabeth [Verfasser]. "New platforms for optical biosensing / Stefanie Elisabeth Ahl." 2007. http://d-nb.info/985594470/34.
Повний текст джерелаLiu, G. W., and 劉廣惟. "Development and Application of nano optical probe on biosensing." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/82229727331304389132.
Повний текст джерела國立臺灣海洋大學
光電科學研究所
94
Recently, the biosensing has attracted much attention in twenty-one century. SPR has drawn extensive attention for application in biosensing, but traditional SPR setup is typically bulky, expensive and requires a large amount of sample solution. In this thesis, we have performed the new SPR sensing devices which have many advantages such as easily fabrication and high capability. We used nano optical probe to induce surface plasmon resonance and measure the specimen by SPR. The numerical simulations are performed by using FDTD method. The result of FDTD simulations indicate the SPW generated near the probe tip. Finally, we used BSA binding on nano optical probe, and it was concluded that the biomedical application have verified by measuring the antigen-antibody interactions.
Fan, Ching-Yu, and 范瀞予. "Measurement Analysis and Biosensing Applications of Optical Microcantilever Sensor." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/60927516130935267205.
Повний текст джерела國立臺灣大學
土木工程學研究所
104
With the development of micro- and nanofabrication technique, a variety of chemical or physical responses of molecular adsorption can be translated into measurable deformation and detected by tiny devices, including doubly clamped beams, membrane, and cantilever, who has one of dimensions in micro- or nanoscale. These devices are referred to as nanomechanical biosensors. With the advantages of ease of measurement and high sensitivity, nanomechanical biosensors are expected to play a promising role in the field of biosensing. In this thesis, we focus on development of the optical microcantilever sensor, including the calibration method, measurement analysis, and the application in the study of chain length effect of self-assembled monolayer (SAM) and drug detection. Firstly, based on the geometrical method, a calibration between signals of the position sensitive detector (PSD) and deflections of the microcantilever is obtained. By utilizing the concept of error propagation, the standard error of deflections calibrated from the PSD signals are defined. Secondly, experiments on the chain length effect of SAM under temperature change are conducted with the proposed calibrated method. Finally, the drug detection of valproic acid is performed with the optical microcantilever sensing platform proposed herein. In this study, we successfully developed a calibration method of measurements, with the definition of accuracy, for the optical microcantilever sensor. The factors resulting in measurement error were found through the measurement analysis. In the experiments of the chain length effect of SAM, we found that the relationship between chain length of SAM and thermal-induced differential surface stress had a turning point when chain length was equal to six. A similar trend was identified when we conducted simulations using Molecular Dynamics simulation (MD). Further calculations of entropy and enthalpy were performed and we found that the change of thermal-induced differential surface stress with the change of chain length was governed by the competition of entropy and enthalpy. In the final part of this thesis, the detection of valproic acid by using the optical microcantilever sensor was performed. The response of molecular adsorption cannot be distinguished from the noise. However, the methods and the standard steps of conducting drug detection experiments were established.
Kaur, Kanwarjeet. "Optical Biosensing Using Localized Surface Plasmon Resonance of Gold Nanoparticles." Thesis, 2011. http://hdl.handle.net/10012/5983.
Повний текст джерелаSANG, JIA-YU, and 宋家裕. "Development of Reflection-Based Optical Waveguide Particle Plasmon Resonance Biosensing Platforms." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/68801620211485505316.
Повний текст джерела國立中正大學
化學暨生物化學研究所
104
The objectives of this work are to develop two novel multiplex chemical and biochemical sensing platforms, namly a reflection-based tubular waveguide particle plasmon resonance (RTW-PPR) biosensing platform, and a reflection-based fiber optic particle plasmon resonance (RFO-PPR) biosensing platform. The principle of inventions are based on measuring the light intensity after consecutive total internal reflections (TIRs) along a noble metal nanoparticles-modified waveguide (tube or optical fiber), wherein the evanescent wave excites the particle plasmon resonance of the nanoparticles at the reflection interface. When a noble metal nanoparticle is influenced by the change of the refractive index on its surrounding environment, its particle plasmon resonance condition will change. This phenomenon can be used as the basis of chemical and biological sensing. In the first part :we used Poly(methyl methacrylate) PMMA as waveguide material to form a tubular waveguide and utilized 3-mercaptopropylsilatrane (MPS) to reduce the modification time. A variety of experiments were carried out to validate the sensitivity and refractive index resolution of the sensing platform. Using different weight percent of sucrose in pure water as samples, a refractive index resolution of 4.34×10-5 RIU and a sensor sensitivity of 5.39 RIU-1 have been achieved by the platform. In the biochemical detection experiments, OVA was used to functionalize the gold nanoparticle in order to detect anti-OVA. Results show that the calibration curve is linear (R2>0.99) and the limit of detection (LOD) is about 4.64×10-6 g/mL (3.09×10-8 M). In the second part:the RFO-PPR platform has achieved the absorbance sensitivity of 4.83 AU/RIU-1 and the sensor resolution of 4.6×10-5 RIU by using gold nanospheres as the sensing element. By the similar configuration, but using gold nanorods as the sensing element, the absorbance sensitivity of 3.81 AU/RIU-1 and the sensor resolution of 3.7×10-5 RIU have been achieved. In the biochemical detection experiments, DNP was used to functionalize the gold nanorods in order to detect anti-DNP antibody. Results show that the calibration curve is linear (correlation coefficient >0.99) and the detection limit is less than 3.88×10-10 M.
Lin, Yi-Chang, and 林奕昌. "Detection of Optical Biosensing Arrays Using White Light Fourier Transform Method." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/52049592017020892257.
Повний текст джерела國立暨南國際大學
應用材料及光電工程學系
102
In recent years, surface plasmon resonance technique has been widely used in optical sensing. Optical biosensors detect the binding energy between bio-molecules using the resonance or propagation behavior of light under different environments. Conventionally, the optical biosensor based on surface plasmon resonance needs an extra prism to couple light to the sensor. Complicated and accurate adjustment is required for phase matching between the surface plasmon wave and the incident light. In recent years, some research works have shown that surface plasmon wave can be excited when light passing through the periodic metallic nanostructures directly, without coupling via a prism. In this study, the optical sensor is designed as a chip-based gold nanoslit array structure. A TM-polarized light is normally incident on the gold nanoslit array with the analyte on top of it. The surface plasmon wave is then excited on the gold surface. The transmission spectrum is measured to detect the interaction between sensing analytes through analyzing the resonance wavelength shifts or intensity changes of the spectrum. The gold nanoslit array is designed as a two-by-two matrix, with slit period 500nm, structure area 5 mm×5 mm, slit depth 150 nm, and the slit width 60 nm. The sample was encapsulated with acrylic as a microfluidic chip, which stabilizes the environment during liquid injection. The experimental set up combines a bright field microscopy with a white-light Fourier spectrometer based on a Michelson interferometer system. In the Michelson interferometer system, the optical path difference is controlled between 0 to 35 μm. The interference pattern is recorded by a charge-coupled-device (CCD) camera as an interferogram. The interferogram is later transformed into spectrum by Fourier analysis using MatlabR software. In this work, the gold nanoslit array was covered by glycerin solution with different concentration. The refractive index of glycerin solution varies from 1.33 to 1.38. Transmission spectra under different environmental index are detected. The wavelength sensitivity, defined as resonance wavelength changes with refractive index, is measured as 213 nm/RIU (refractive index unit). Since the linearity of wavelength sensitivity is not good enough, we further use a center mass method to improve the linearity. The measured wavelength sensitivity under center mass method is 130 nm/RIU. Although the sensitivity is lower, a better linearity is obtained. Moreover, the intensity sensitivity, defined as intensity changes with refractive index, is measured as 99 %/RIU. To increase intensity sensitivity, a multispectral integration method is used. An increased sensitivity of 2078 %/RIU is obtained. Furthermore, the detection of the binding between bovine serum albumin (BSA) and bovine protein antibodies (anti-BSA) are measured, with BSA concentration 6.06 μM and anti-BSA 60 nM. The multispectral integration method is used to analyze the experimental results for better intensity sensitivity. Experimental results show that the binding between BSA and anti-BSA can be easily detected by the spectra change. Key words:Surface plasmon resonance, White-light Fourier spectrometer
Fan, Shu-Yu, and 范書毓. "Fiber-optical biosensing platform for quantifying cell population or tissue level activities." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/87907459896605344892.
Повний текст джерела國立中興大學
生醫工程研究所
101
A fiber-optical biosensing platform in coordination with localized fluidic delivery to perform topical cell-scale assay inside living tissue or organs in vivo. The deviation from the cell density and spatial configuration in the vicinity of sensor probe can cause error in threshold value determination, which is difficult for the conventional extrinsic catheter fiber-optic designs to predict or calibrate for in vivo applications. The strategy for correcting / calibrating the difference from the spatial issue is to stain the cells with two fluorescent agents. The 1st fluorophore (indicator) will has a known effect on all cells which is not affected by the conditions (“normal / control” or “treated” with desired physiological changes) of the cells; the signal from the indicator will be considered as “baseline” reflecting each independent measurement with specific density and configuration of the cells in the vicinity of sensor tip. The 2nd fluorophore (reporter) will exhibit level of physiological change on “treated” cells. The significance of the physiological changes on individual cell will be evaluated by the ratio of the two fluorescent signals (reporter / indictor) to report the normalized deviation between “control” and “treated” cell population. In this research, we applied the fiber-optic sensor platform in monitoring chemotherapeutic, cyclophosphamide (CPA), induced 3D-distributed MCF-7 cell (human breast carcinoma cell line) apoptosis for verifying the feasibility and capability of the system in monitoring cell population or tissue level activities in vivo. In the 1st stage of the development, 25mM CPA was found to enhance apoptosis of cancer cells in cell viability (MTT) assay under 2D cell culture, increase the adsorption amount of fluorophore FM 1-43 on apoptotic cells, but had no effect on the adsorption amount of indocyanine derivatives on apoptotic cells. In the 2nd stage of study, the fiber-optic sensing platform monitored the kinetics of fluorescence changes around the micro-environment of sensor tip with a 200μm i.d. optical fiber, conveying the excitation and returning emission, and a 325μm i.d. microcapillary initially delivering fluorophore, 750nl 300 μm naphthalene asymmetric indocyanine derivative (Cpd.B), in indicating the spatial distribution (density) of cells in the tissue-mimic system, following by delivering fluorophore, 750nl 160μM FM1-43, in demonstrating the apoptotic activity induced by CPA after calibrated with the previous Cpd.B reported cell distribution indicating signal. When cell density exceeded 107cells/ml, the increase percentage of peak values in dynamic fluorescent change pattern of Cpd.B were in proportion to cell densities, but not correlated statistically with CPA-induced apoptotic activities. However, the increase percentages of peak values in dynamic fluorescent change pattern of FM 1-43 were in proportion to both cell densities and CPA-induced apoptotic activities. In the cell density between 107-108cells/ml, the increase percentage of peak values in dynamic fluorescent change pattern from FM 1-43 interacted with CPA-induced apoptotic cells were 2.1-3.3 folds of peak values from FM 1-43 interacted with control cell. When sequentially interacted with Cpd.B and FM 1-43, the ratios from increase percentage of peak values of FM 1-43 divided by increase percentage of peak values of Cpd.B were around 0.71-1.58 in the control cells with different densities, while the ratios from CPA-induced apoptotic cells were around 3.62-10.68. The value of ratio could be applied as indication of apoptotic activity without interference of spatial distribution of cells. The preliminary result verified the feasibility and capability of the system in monitoring cell population or tissue level activities in vivo.
Waly, Noha [Verfasser]. "Optimization of core-shell nanoparticle layers for optical biosensing / presented by Noha Waly." 2011. http://d-nb.info/1010842811/34.
Повний текст джерелаLin, Chi-Hao, and 林琪皓. "Electro-Optical and Capacitive Measurements for Quantitative Analysis of Liquid-Crystal-Based Biosensing." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/u7jtxk.
Повний текст джерела國立交通大學
影像與生醫光電研究所
104
The texture observation has long been the core technique in liquid crystal (LC)-based biosensing. However, quantitative analysis of this method for determining the biomolecular concentration can hardly be realized since the existence of biomolecules immobilized on the substrate is examined in accordance with the brightness of optical textures. In considering with the electrical response of birefringence and dielectric anisotropy of LC materials, in this study, two approaches―electro-optical and capacitive measurements, are proposed specifically for quantitative analysis of LC-based biosensing. Here, the biomolecule used is bovine serum albumin (BSA), a protein standard commonly used in the assay of protein concentration, and the LC material used as the sensing platform is highly birefringent HDN with positive dielectric anisotropy. Experimental results indicate that the detection limit of the BSA concentration is 106 g/ml for the electro-optical measurement and is 109 g/ml for the electric capacittance measurement. The difference between the detection limit of the two measurements stems from the method used for the immobilization of BSA on the DMOAP-coated substrates. For the electro-optical measurement, the BSA biomolecules were spin-coated on the substrate to reduce percentage error on the calculation of phase retardation, attributable to the light scattering. By measuring voltage-dependent transmission (VT) curves and calculating phase retardations of experimental samples, we propose an N value, defined as the ratio of the effective refractive index of the sample to the birefringence of the LC material, to quantitatively estimate the influence of the BSA concentration on the orientation of LC molecules. On the other hand, samples used for the capacitive measurement were prepared using droplets to attach protein on the substrates. Experimental results based on the voltage-dependent capacitive (VC) curves render information on the maximum capacitance Cmax and the capacitance difference ΔC. Furthermore, a quantitative value defined as the ratio of Cmax to ΔC of a sample driven by external AC voltages is calculated to analyze the relationship between the LC tilted angle and the BSA concentration.
"Dependence of surface plasmon polaritons on the geometry of periodic metallic nanostructures and Its application on biosensing." 2012. http://library.cuhk.edu.hk/record=b5549441.
Повний текст джерела在第一部份中,表面等離子體模式被分為非簡並模式(m,O) 和簡並模式(m,±n)兩種情況分別加以討論。首先,結合實驗結果和理論模型,我們對非簡並模式的衰減壽命和激發效率進行了研究。通過光干涉光刻法和薄膜沉積技術,一系列不同幾何結構的洞陣列樣品被製備出來,且這些樣品具有很高的重複性。利用角分辨色散關係進行模式識別以及確定這些模式的衰減壽命和激發效率。通過調整起偏器和檢偏器的相對方向,表面等離子體模式的非輻射和輻射衰竭均可加以研究。結果發現,衰減壽命強烈依賴於單洞的幾何結構,而且這種行為可以用簡單的靜電模型并考慮高階修正加以解釋。從非輻射衰減和輻射衰減平衡的角度出發,激發效率對幾何結構和共振波長的依賴性也可以理解。對於簡并模式,由於存在稱合,對稱模式和反對稱模式分別被p 偏振激發和S偏振激發。它們的對稱性和對於衰減壽命和共振波長的修正可以用干涉法和模式耦合理論來理解。最後,利用多模耦合方程,我們對色散關係圖譜隨著洞深度增加而演化的情況也進行了探討。
第二部份對基於表面等離子體共振的生物傳感器與陣列幾何結構的關係進行了研究。結果發現,激發效率和衰減壽命對表面等離子體共振傳感器的分辨率都起到了關鍵性作用。在共振中,峰值高度和帶寬主要由表面等離子體的衰減速率控制。較低的衰減速率導致較清晰的峰值線型,從而產生較高的傳感器分辨率。因此,通過調整陣列的幾何結構以產生非常低得輻射衰減速率,表面等離于體共振傳感器的品質因數可高達104.8/RIU ,這已經超過了基於梭鏡和納米粒子對應器件的性能表現。
Surface plasmon polaritons (SPPs) generate a strong localized electromagnetic field on metal surface and thus are promising for nano- and bio-photonics including high performing light-emitting diodes and photovoltaic cells, super-high resolution optical imaging, ultra-high sensitive bimolecular detection, etc. In particular, the application of SPPs on surface plasmon resonance (SPR) biosensor has drawn much more attention recently because of the attempt to realize single molecule detection in medical diagnosis.
This thesis contains two parts. The first part focuses on studying the basic plasmonic properties of two-dimensional periodic hole arrays while the second part concentrates on the application of hole arrays on SPR sensing.
In the first part, SPPs modes on hole arrays are classified into nondegenerate mode (m, 0) and degenerate mode (m, ±n). For nondegenerate mode, its decay lifetime and generation efficiency are studied both experimentally and theoretically. By combining interference lithography and thin film deposition, a set of arrays with a wide range of geometry has been fabricated with high reproducibility. The dispersion relations of arrays are studied by angle-dependent reflectivity for mode identification and detenninations of SPP decay lifetime and generation efficiency. In particular, through orienting the polarization of the specular reflection either parallel or orthogonal to that of the incidence, we can access both the nonradiative and radiative decays of SPPs at different resonance wavelengths. As a result, it is found that decay lifetime is strongly dependent on the geometry of single hole and its behaviors can be understood by using a simple quasi-static model taking into account of the higher order correction as well as numerical simulation deduced by finite-difference timedomain. The dependence of generation efficiency on hole geometry or resonance wavelength can be understood in tenns of trade-off between nonradiative and radiative decay rates. Once these two decay rates equals to each other, the optimum generation efficiency is realized and the field enhancement gets the maximum. And the optimum parameters can be achieved by adjusting the hole geometry or the resonance wavelength. For degenerate mode, due to the coupling between (m, +n) and (m, -n) modes, a symmetric and an anti-symmetric modes are excited under pand s-polarized excitation, respectively. Their symmetries and modifications to the decay lifetime and resonance wavelength can be understood by using the interference method and coupled mode theory. Finally, generalized coupled mode equations are employed to know about the evolution of dispersion relation as hole depth increases.
The dependence of SPR biosensor on the generation and decay of SPPs are studied in the second part. Both the generation efficiency and decay lifetime of SPPs are found to be critical in governing the resolution of SPR biosensor. In SPR, the peak height and linewidth are primarily controlled by the decay rate of SPPs. Lower decay rate leads to sharper peak profile, which results in higher SPR resolution. Therefore, by tailoring the geometry of hole arrays to achieve a very low radiative decay rate, a SPR biosensor with figure of merit (FOM) reaching l04.8/RIU can be realized, which surpasses those of prism and nanoparticle counterparts.
Detailed summary in vernacular field only.
Detailed summary in vernacular field only.
Detailed summary in vernacular field only.
Zhang, Lei = 表面等離子體激元對於週期性金屬納米結構幾何形狀的依賴性及其在生物傳感中的應用 / 張磊.
"November 2011."
Thesis (Ph.D.)--Chinese University of Hong Kong, 2012.
Includes bibliographical references (leaves 116-125).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Abstract also in Chinese.
Zhang, Lei = Biao mian deng li zi ti ji yuan dui yu zhou qi xing jin shu na mi jie gou ji he xing zhuang de yi lai xing ji qi zai sheng wu chuan gan zhong de ying yong / Zhang Lei.
Chapter Chapter1 --- Introduction --- p.1
Chapter Chapter2 --- Theoretical Background --- p.7
Chapter 2.1 --- Maxwell's equations --- p.7
Chapter 2.2 --- Classic electromagnetic theory for dielectric properties of matter --- p.9
Chapter 2.3 --- Surface plasmon polaritons at the dielectric/metal interface --- p.11
Chapter 2.4 --- Excitation of surface plasmon polaritons --- p.16
Chapter 2.4.1 --- Prism coupling --- p.17
Chapter 2.4.2 --- Grating coupling --- p.17
Chapter 2.5 --- lones calculus --- p.19
Chapter 2.6 --- Finite-difference time-domain method --- p.22
Chapter Chapter 3 --- Sample Preparation --- p.25
Chapter 3.1 --- Interference lithography --- p.25
Chapter 3.1.1 --- Substrate preparation --- p.26
Chapter 3.1.2 --- Exposure --- p.27
Chapter 3.1.3 --- Pattern development --- p.30
Chapter 3.2 --- Thin film deposition --- p.30
Chapter Chapter 4 --- Experimental Setups and Calibration --- p.33
Chapter 4.1 --- Experimental setup for measuring dispersion relation --- p.33
Chapter 4.2 --- Experimental setup calibration --- p.35
Chapter 4.2.1 --- Calibration of spectrometer --- p.36
Chapter 4.2.2 --- Calibration of movement stages --- p.38
Chapter 4.3 --- Data presentation for dispersion relation --- p.40
Chapter 4.4 --- Summary --- p.41
Chapter Chapter 5 --- Understanding of Fundamental Properties of SPPs --- p.43
Chapter 5.1 --- Excitation of SPPs on 2D hole arrays --- p.44
Chapter 5.2 --- Properties of non degenerate modes and theoretical explanation --- p.53
Chapter 5.2.1 --- Dependence of lifetime on hole geometry and theoretical explanation --- p.55
Chapter 5.2.2 --- Dependence of generation efficiency on hole geometry and theoretical explanation --- p.63
Chapter 5.3 --- Properties of degenerate modes and theoretical explanation --- p.70
Chapter 5.3.1 --- Dependence of properties of degeneration modes on hole geometry by FDTD --- p.72
Chapter 5.3.1.1 --- (0, ±l)[subscript s,a] modes --- p.72
Chapter 5.3.l.2 --- (-1, ±l)[subscript s,a] modes --- p.76
Chapter 5.3.2 --- Understanding of excitation of degenerate modes by using interference method --- p.79
Chapter 5.3.3 --- Understanding of coupling between degenerate modes by using coupled mode theory --- p.85
Chapter 5.4 --- Evolution of dispersion relation as hole depth increases --- p.90
Chapter 5.5 --- Summary --- p.95
Chapter Chapter 6 --- Surface Plasmon Resonance Based Label Free Biosensor --- p.98
Chapter 6.1 --- Basics of surface plasmon resonance (SPR) based biosensor --- p.98
Chapter 6.2 --- State-of-the-art SPR biosensor --- p.101
Chapter 6.3 --- SPR biosensor by using 2D metallic hole arrays --- p.102
Chapter 6.4 --- Summary --- p.111
Chapter Chapter 7 --- Conclusions --- p.112
References --- p.116
Publications --- p.126
Lee, Kuang-Li, and 李光立. "The Properties of Optical Wave in Periodic Metallic Nanostructures and Its Application in Biosensing." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/68727467947839775407.
Повний текст джерела國立臺灣大學
光電工程學研究所
96
In this dissertation, a detailed study of the optical properties of single metallic nanoslit and multiple metallic nanoslits is presented. In experiment, high sensitive, label-free, high-throughput, reusable, and chip-based biosensor arrays based on surface and gap plasmon resonance were fabricated and tested. In the study of optical properties of metallic nanostructures, metallic nanoslits with periods varying from 400 to 900 nm and widths ranging from 20 to 200 nm were fabricated on a thin gold film using e-beam lithography and reactive ion etching. The thickness of the gold film is varying from 100 to 200 nm and the area of the metallic nanoslit array is chosen as 100 µm×100 µm. Experimental results show that gap plasmon resonance in the slit generates a peak with a broader full-width half-maximum in the transmission spectrum and the peak wavelength is affected by slit width and film thickness. That’s due to surface plasmon resonances outside the slit play a negative role in optical transmission and present a dip in the transmission spectrum. Besides, a peak accompanying an SPR dip can be predicted by Rayleight anomaly. The peaks or dip is sensitive to the refractive index change of the environment and can be applied in biological or chemical sensing. In the application of biological detection, SPR biosensor achieved a detection sensitivity of up to 740 nm per refractive index unit and an antigen–antibody interaction experiment in an aqueous environment verified the sensitivity in a surface binding event. The surface sensitivities of surface and gap plasmons were compared by coating a thin SiO2 film and different biomolecules on the nanoslit arrays. Experimental results show gap plasmons are more sensitive than conventional surface plasmons. The gap plasmons can detect a 0.05 nm-thick SiO2 film and ~4 Da-sized biomolecules attached to the surface when the resolution of a spectrometer is 0.1 nm. Besides, its detection sensitivity is increased with the decrease of the slit width. The gap plasmon is one order of magnitude more sensitive than the surface plasmon for slit widths smaller than 30 nm. In the 13-nm-diameter gold nanoparticle detection, gap plasmon is 3 times more sensitive than surface plasmon. A detection sensitivity of 1 particle/µm2 was achieved with a 0.1 nm wavelength shift or a 0.2% peak intensity change. This sensitivity is comparable with that of the fluorescent dyes ~0.5 fluors/µm2 used in DNA microarrays. Such a high sensitivity is attributed to the large overlap between biomolecules or nanoparticles and nanometer-sized gap plasmons.
Ho, Po-Ching, and 何柏慶. "Localized Surface-Plasmon-Resonance Biosensing System by Electro-Optical Modulation in the ATR Configuration." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/m78v64.
Повний текст джерела國立臺北科技大學
光電工程系研究所
97
In this dissertation, we present an electro-optically modulated localized surface plasmon resonance biosensing system in the attenuated-total-reflection configuration, which can be used to measure the concentration of biochemical material. The sensing chip consists of a sensing region and one pair of electrodes on the ridge structure. When the voltage is applied on the electrodes, the electric field produced in lithium niobate electro-optically modulates the refraction index and changes the wave vector of the incidence lightwave. In this study, we use two kinds of measurement configurations, including optical intensity and phase shift configuration. Excited layer of surface plasmon formed by gold nanoparticle on gold film is adopted in the sensor. Human serum albumin (HSA) produced by the self-assembling method is used as the sensing layer to real-time sense the concentration of beta-blocker, which is a kind of medicine for heart disease. During the sensing measurement, the concentration of beta-blocker can be determined by the relation between the reflection intensity (or the phase shift) and the voltage. In comparison with the conventional SPR sensing, the proposed SPR sensing system has many advantages, such as: high accuracy, high sensitivity, and easy operation. In the future application, the presented biosensing system can be utilized to measure the interaction between HSA and medicines molecule for understanding the interaction mechanism of medicine in the human body.
Enders, Dominik [Verfasser]. "Surface enhanced infrared absorption on Au nanoparticle films for optical biosensing / presented by Dominik Enders." 2006. http://d-nb.info/97824673X/34.
Повний текст джерела