Дисертації з теми "Operonic regulation"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Operonic regulation".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Patterson, Kathryn Grace. "Gene regulation in the lac operon." Thesis, Montana State University, 2009. http://etd.lib.montana.edu/etd/2009/patterson/PattersonK0809.pdf.
Повний текст джерелаLazarus, Linda Ruth. "The role of FIS in tyrT transcriptional regulation." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259766.
Повний текст джерелаVichivanives, Padungsri. "Transcriptional regulation of the Rhodobacter Capsulatus CO? fixation operons /." The Ohio State University, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488205318510482.
Повний текст джерелаNold, Niklas. "Untersuchungen zur Regulation des sol-Operons in Clostridium acetobutylicum." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-65443.
Повний текст джерелаClarke, Simon Richard. "Regulation of the bla operon in Staphylococcus aureus." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326077.
Повний текст джерелаMin, Kyung-Tai. "Regulation of the spoIIA operon in Bacillus subtilis." Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333440.
Повний текст джерелаLow, Yuen Li. "Metal regulation of the E. faecalis efaCBA operon." Thesis, University of Bath, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.760823.
Повний текст джерелаDole, Sudhanshu. "Multiple level regulation of the Escherichia coli bgl operon." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963645978.
Повний текст джерелаDavies, Ian J. "Transcriptional regulation of the qua operon of Escherichia coli." Thesis, University of Southampton, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314696.
Повний текст джерелаYuen, Hiu-fung. "A study of the catabolite repression of the dehalogenase IVa gene of Burkholderia cepacia MBA4." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B30682113.
Повний текст джерелаStephen, Robert John. "A study of the proU operon of Salmonella typhimurium." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318587.
Повний текст джерелаGould, Phillip Spencer. "Regulation and role of the three chaperonin operons of Rhizobium leguminosarum." Thesis, University of Birmingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273921.
Повний текст джерелаColeman, Struan Howard. "The regulation of the rpmB,G operon of Escherichia coli." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260156.
Повний текст джерелаShevtsov, Mikhail Borisovich. "Structural studies of the trp operon regulation in Bacillus subtilis." Thesis, University of York, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.507488.
Повний текст джерелаYuen, Hiu-fung, and 阮曉峰. "A study of the catabolite repression of the dehalogenase IVa gene of Burkholderia cepacia MBA4." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30682113.
Повний текст джерелаSteven, Blaire. "Regulation and expression of the mdh-sucCDAB operon of Sinorhizobium meliloti." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80880.
Повний текст джерелаNorman, Richard Albert. "Signal transduction in the regulation of operon expression in Pseudomonas aeruginosa." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298366.
Повний текст джерелаWang, Baomin. "Studies on the Regulation of the Assimilatory Nitrate Reductase Operon in Azotobacter vinelandii." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/195085.
Повний текст джерелаHillebrand, Annette. "Transkriptionsfaktor- und wachstumsabhängige Regulation der sieben verschiedenen ribosomalen RNA-Operons in Escherichia coli." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963523325.
Повний текст джерелаTover, Andres. "Regulation of transcription of the phenol degradation pheBB operon in pseudomonas putida /." Online version, 2005. http://dspace.utlib.ee/dspace/bitstream/10062/1343/5/tover.pdf.
Повний текст джерелаPoon, Karen Kuen Huan. "Regulation of Bacillus subtilis glucitol operon, characterization of a transcription activator, GutR." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0018/NQ54804.pdf.
Повний текст джерелаTesfa-Selase, Fisehaye. "Regulation of the gua operon of Escherichia coli by the DnaA protein." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385344.
Повний текст джерелаBotha, Jeanine. "The regulation and function of the ESAT-6 gene cluster operons of Mycobacterium tuberculosis." Thesis, Link to the online version, 2006. http://hdl.handle.net/10019/505.
Повний текст джерелаNeubert, Miranda J., Elizabeth A. Dahlmann, Andrew Ambrose, and Michael D. L. Johnson. "Copper Chaperone CupA and Zinc Control CopY Regulation of the Pneumococcal cop Operon." AMER SOC MICROBIOLOGY, 2017. http://hdl.handle.net/10150/626457.
Повний текст джерелаTagliabue, L. "THE SUBTLE BIOFILM REGULATION IN ESCHERICHIA COLI: CSGD AND THE YDDV-DOS OPERON." Doctoral thesis, Università degli Studi di Milano, 2010. http://hdl.handle.net/2434/160739.
Повний текст джерелаBailey, Marc J. A. "HlyT, a transcriptional regulator of the Escherichai coli hemolysin operon." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259542.
Повний текст джерелаDeng, Liyu, and 鄧麗瑜. "Exploration of the transcription factors that regulate the expression of the haloacid operon in Burkholderia caribensis MBA4." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208618.
Повний текст джерелаpublished_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy
Allen, Darin. "Transcriptional regulation of the dlt operon in Enterococcus faecalis and further characterization of a dlta mutant." Thesis, Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/783.
Повний текст джерелаGrainger, David Christopher. "Transcriptional regulation in the Escherichia Coli melibiose operon : interactions of the MeIR activator protein." Thesis, University of Birmingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410284.
Повний текст джерелаLee, Ka Man. "Regulation of expression of the type IV B pili-encoding operon of salmonella typhi /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BICH%202003%20LEE.
Повний текст джерелаIncludes bibliographical references (leaves 110-127). Also available in electronic version. Access restricted to campus users.
Gliese, Nicole. "Untersuchungen zur Regulation des pqq-Operons und anderer Komponenten des Ethanol-oxidierenden Systems von Pseudomonas aeruginosa." Berlin mbv, Mensch-und-Buch-Verl, 2009. http://d-nb.info/998052116/04.
Повний текст джерелаVélez, Acevedo Rosuany. "Analysis of the Regulation of the Transferrin Iron Acquisition System in Neisseria gonorrhoeae." VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/1955.
Повний текст джерелаJones, Melissa Kolsch. "Regulation of phase variation and deletion mutation in the Vibrio vulnificus group 1 CPS operon." [Gainesville, Fla.] : University of Florida, 2006. http://purl.fcla.edu/fcla/etd/UFE0013839.
Повний текст джерелаQuinlivan-Repasi, Vanessa H. "Transcriptional Regulation of the Acetone Carboxylase Operon via Two-Component Signal Transduction in Helicobacter pylori." W&M ScholarWorks, 2012. https://scholarworks.wm.edu/etd/1539626927.
Повний текст джерелаCol, Bekir. "Regulation of Fructose 1,6-bisphosphatase II (GlpX) Gene Expression in Escherichia coli." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/11282.
Повний текст джерелаPh. D.
Ucci, Amanda Piovesan. "Participação da ORF XAC3629 na regulação do operon de resistência a cobre copAB em Xanthomonas citri subsp. citri /." Araraquara : [s.n.], 2009. http://hdl.handle.net/11449/88003.
Повний текст джерелаBanca: Jesus Aparecido Ferro
Banca: Julio Cezar Franco de Oliveira
Resumo: Xanthomonas citri subsp. citri (Xcc) é uma bactéria Gram-negativa responsável pelo o cancro cítrico. A doença causada por este fitopatógeno constitui numa das mais graves doenças da citricultura brasileira, cujas medidas de controle capazes de eliminar completamente a doença são ineficientes até o momento. O sequenciamento e anotação do genoma de Xcc juntamente com estudos relacionados à genômica funcional nesta bactéria realizados em nosso laboratório mostraram a existência, na bactéria, de alguns genes (copA e copB) que codificam proteínas envolvidas com o mecanismo de resistência a cobre (CopA e CopB). Os genes que codificam ambas proteínas estão organizados em um operon, cuja transcrição é induzida por cobre e é específica ao metal. Componentes a base de cobre são comumente usados na agricultura como agentes microbicidas a fim de reduzir a severidade desta e também de outras doenças nas plantas. Contudo, a eficácia do cobre está sendo reduzida pelo aparecimento de linhagens de bactérias resistentes a este metal, portanto, estudos visando a compreensão dos mecanismos moleculares envolvidos na resistência a cobre em Xcc são de grande importância. Uma pequena ORF (XAC3629) que codifica uma proteína hipotética de 152 aminoácidos e que se localiza upstream aos genes copAB foi identificada. Neste trabalho foi estudado o papel da ORF na regulação do operon copAB. Linhagens mutantes contendo a ORF XAC3629 interrompida por um transposon (XAC3629::Tn), previamente construídas no laboratório, foram avaliadas em relação ao crescimento em meio contendo diferentes concentrações de cobre. Estas linhagens mostraram perda da resistência quando o metal foi adicionado ao meio, mesmo em baixas concentrações (0,25 mM), sugerindo seu envolvimento na regulação do operon de resistência a cobre copAB. Células da linhagem selvagem ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium that causes citrus canker, one of the most serious diseases in Brazilian crops, and the ways to eliminate it are not efficient. After sequencing and annotation of the Xcc genome our laboratory started to perform functional genomic studies in Xcc. Previous studies identified two genes (copA and copB) that encode proteins involved in the copper resistance mechanism (CopA and CopB). The genes are organized in an operon whose transcription is induced and specific to copper. Copper compounds are commonly used in agriculture in order to control plant diseases, however the copper effectiveness is being reduced due to the emergence of copper resistant bacteria strains. Therefore, studies related to copper resistance mechanism in Xcc are very relevant. A short ORF (XAC3629) which encodes an 152 amino acids hypothetical protein was identified upstream the operon copAB. In this work the role of the ORF in the operon regulation was studied. Mutant strains containing the ORF XAC3629 interrupetd by a transposon (XAC3629::Tn) were previously constructed in our laboratory and in this work their growth were evaluated in a medium containing different copper concentration. The mutant strains were unable to grow when the metal was added to the medium, even at low concentration (0.25 mM) suggesting that the ORF XAC3629 may have a role in regulating the operon copAB. Wild type Xcc cells are able to grow up to 1 mM copper concentration. Attempts to construct an ORF XAC3629 knocked out strain were performed but this mutant strain was not obtained. Two XAC3629::Tn mutants strains were transformed with a plasmid construction containing the ORF XAC3629 entire sequence plus its flanking regions. The results did not show functional complementation (recovery of the copper resistance when cells were grown in medium containing ... (Complete abstract click electronic access below)
Mestre
Weissenborn, Deborah Louise. "Regulation of the glpFK operon of Escherichia coli K-12 and characterization of it gene products." Diss., Virginia Tech, 1990. http://hdl.handle.net/10919/39937.
Повний текст джерелаBröms, Jeanette. "Type III secretion- the various functions of the translocon operon in bacterial pathogenesis." Doctoral thesis, Umeå University, Molecular Biology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-331.
Повний текст джерелаIn order to establish colonisation of a human host, pathogenic Yersinia use a type III protein secretion system to directly intoxicate host immune cells. Activation of this system requires target cell contact and is a highly regulated process. Both the intoxication and regulation events depend on the lcrGVHyopBD translocon operon, which is highly conserved in many bacterial pathogens. In this study, the role of individual operon members was analysed and functional domains identified by using the highly homologous pcrGVHpopBD operon of P. aeruginosa as a comparative tool.
Yersinia spp. and P. aeruginosa were shown to form translocation pores of a similar size that promoted equally efficient protein delivery. A strong dependency on interactions between native translocator(s) in protein delivery was revealed, suggesting that each pathogen has delicately fine-tuned this process to suit its own infection niche. In particular, the C-terminus of YopD was shown to possess functional specificity for effector delivery in Yersinia that could not be conferred by the comparable region in homologous PopD. Moreover, a role for LcrV and PcrV in substrate recognition during the protein delivery process was excluded.
The N-terminus of LcrH was recognized as a unique regulatory domain, mediating formation of LcrH-YscY regulatory complexes in Yersinia, while equivalent complexes with analogous proteins were not formed in P. aeruginosa. These results compliment the idea that a negative regulatory pathway involving LcrH, YopD, LcrQ and YscY is unique to Yersinia.
Finally, PcrH was identified as a new member of the translocator class of chaperones, being essential for assembly of a functional PopB/PopD mediated translocon in P. aeruginosa. However, in contrast to the other members of this family, PcrH was dispensable for type III regulation. Moreover, both LcrH and PcrH were shown to possess tetratricopeptide repeats crucial for their chaperone function. One tetratricopeptide repeat mutant in LcrH was even isolated that failed to secrete both YopB and YopD substrates, even though stability was maintained. This demonstrates for the first time that LcrH has a role in substrate secretion in addition to its critical role in promoting substrate stability.
Hammar, Petter. "lac of Time : Transcription Factor Kinetics in Living Cells." Doctoral thesis, Uppsala universitet, Beräknings- och systembiologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-198814.
Повний текст джерелаJonsson, Maria. "Contribution of the outer surface proteins of Borrelia burgdorferi s.l. to the pathogenesis of Lyme disease." Doctoral thesis, Umeå universitet, Mikrobiologi, 1994. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-100569.
Повний текст джерелаDiss. (sammanfattning) Umeå : Umeå universitet, 1994, härtill 5 uppsatser
digitalisering@umu
Ucci, Amanda Piovesan [UNESP]. "Participação da ORF XAC3629 na regulação do operon de resistência a cobre copAB em Xanthomonas citri subsp. citri." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/88003.
Повний текст джерелаFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Xanthomonas citri subsp. citri (Xcc) é uma bactéria Gram-negativa responsável pelo o cancro cítrico. A doença causada por este fitopatógeno constitui numa das mais graves doenças da citricultura brasileira, cujas medidas de controle capazes de eliminar completamente a doença são ineficientes até o momento. O sequenciamento e anotação do genoma de Xcc juntamente com estudos relacionados à genômica funcional nesta bactéria realizados em nosso laboratório mostraram a existência, na bactéria, de alguns genes (copA e copB) que codificam proteínas envolvidas com o mecanismo de resistência a cobre (CopA e CopB). Os genes que codificam ambas proteínas estão organizados em um operon, cuja transcrição é induzida por cobre e é específica ao metal. Componentes a base de cobre são comumente usados na agricultura como agentes microbicidas a fim de reduzir a severidade desta e também de outras doenças nas plantas. Contudo, a eficácia do cobre está sendo reduzida pelo aparecimento de linhagens de bactérias resistentes a este metal, portanto, estudos visando a compreensão dos mecanismos moleculares envolvidos na resistência a cobre em Xcc são de grande importância. Uma pequena ORF (XAC3629) que codifica uma proteína hipotética de 152 aminoácidos e que se localiza upstream aos genes copAB foi identificada. Neste trabalho foi estudado o papel da ORF na regulação do operon copAB. Linhagens mutantes contendo a ORF XAC3629 interrompida por um transposon (XAC3629
Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium that causes citrus canker, one of the most serious diseases in Brazilian crops, and the ways to eliminate it are not efficient. After sequencing and annotation of the Xcc genome our laboratory started to perform functional genomic studies in Xcc. Previous studies identified two genes (copA and copB) that encode proteins involved in the copper resistance mechanism (CopA and CopB). The genes are organized in an operon whose transcription is induced and specific to copper. Copper compounds are commonly used in agriculture in order to control plant diseases, however the copper effectiveness is being reduced due to the emergence of copper resistant bacteria strains. Therefore, studies related to copper resistance mechanism in Xcc are very relevant. A short ORF (XAC3629) which encodes an 152 amino acids hypothetical protein was identified upstream the operon copAB. In this work the role of the ORF in the operon regulation was studied. Mutant strains containing the ORF XAC3629 interrupetd by a transposon (XAC3629
Pinto, Cecilia de Agrela. "The two-component system of a novel copper resistant operon of Marinobacter hydrocarbonoclasticus." Master's thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/10062.
Повний текст джерелаThe majority of bacterial heavy metal resistance systems are regulated by twocomponent signal transduction systems. Stimuli from the environment interact with the histidine kinase, which in turn activates the response regulator by phosphorylation. The effector domain of the response regulator then binds to DNA, eliciting the specific response. Analysis of the Marinobacter hydrocarbonoclasticus genome revealed the presence of genes, copXAB, that code for proteins associated with copper response. The biochemical characterization of the two-component signal transduction system, copSR, is of interest due to the vital role it plays in the regulation of expression of the copXAB operon. The genes that encode for the CopR and CopS_C (cytosolic sensor domain of CopS) proteins were heterologously expressed in E.coli and expression was optimized for the production of soluble protein using LB medium. Due to solubility problems, the genes that code for these proteins were cloned as hexahistidine or glutathione S-transferase fusion proteins. CopR and its domains were optimally expressed at 16°C for 16 and 3 h after induction, respectively, whilst CopS_C was expressed at 37°C during 3 h after induction. Proteins were purified using different chromatographic strategies, most of them using affinity chromatography. The yields of pure protein per liter of growth culture obtained after complete purification from the soluble cellular extract were: 0.14 to 0.23 mg/L for CopR; 0.42 mg/L, CopR_NHis6; for the CopR_CHis6 it was 0.16 mg/L and 4.2 mg/L of CopS_C. The molecular mass of each protein was determined by gel filtration, 31 kDa for CopR, 17.5 kDa for CopR_NHis6, 15.1 kDa for CopR_CHis6 and 38.2 kDa for CopS_C. In the case of CopS_C there is the possibility that a dimer is formed, which should be evaluated. From the evaluation of disulfide bonds, using SDS PAGE and PAGE gels, all proteins or protein domains appeared to be monomers when in the presence of β-MEtOH. Circular dichroism evaluated the state of folding of the CopS_C and CopR proteins, which were shown to be folded in which the α-helix structures predominate. A model structure for CopR was also determined which agrees with this analysis. However, in the case of the CopR domains, the data obtained merely indicate folding, due to the low concentrations of the proteins. Phosphorylation and electrophoresis mobility shift assays of the CopR protein were, for the most part, inconclusive. However, in the absence of BSA, formation of the CopR:DNA complex in a gel filtration column is observed, though requires additional evaluation.
Morante, Estela Ynés Valencia. "Estudo do sistema BlaR/Blal e de dois operons codificando sistemas de efluxo RND em Caulobacter crescentus." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04062012-105252/.
Повний текст джерелаThe aim of this work is to characterize three clusters of genes from the alpha proteobacterium Caulobacter crescentus involved in metal and antibiotics response. We analyzed the cluster comprising genes CC1637-CC1640, which contains a BlaR/BlaI signal transduction system, and we performed a comparative analysis with two RND efflux systems consisting on genes CC2720-CC2725 and CC2388-CC2390, which are probably involved in cadmium and zinc response. Mutant strains for one or two of these genes were obtained, and the study of promoter activity was performed by b-galactosidase activity assays using lacZ as reporter gene. RT-PCR and b-galactosidase activity assays revealed that the CC1638 gene probably does not possess an exclusive promoter, and that the genes CC1637-CC1640 may constitute an operon. The promoter of CC1640 does not respond to H2O2, Cd2+ and Zn2+, but the DCC1640 strain presented low viability in the presence of Cd2+. The DCC1637 and DCC1638 strains showed sensitivity to t-butyl-hydroperoxide. The DCC1640 strain showed sensitivity to the antibiotics CTX and PPT, and b-galactosidase activity assays performed both on plates and liquid medium showed induction of the expression by the presence of antibiotics CTX and CFE. We observed auto-regulation of the operon by the protein encoded by the CC1640 gene (BlaI), which was confirmed by EMSA assays. The presence of BlaR inhibits the binding of the BlaI protein to the promoter, suggesting that both BlaI/BlaR regulate the CC1640 gene promoter. In silico analyses for the TTACGNNCGTAA consensus, located on CC1640 promoter, identified this sequence in promoter regions of other genes. Relative expression analyses indicate that the genes CC1568, CC1230 and CC2661 are regulated by the BlaI protein, suggesting that BlaI regulates the expression of other genes, possibly involved in b-lactamic antibiotics response. b-galactosidase activity assays of the intergenic region CC2720-CC2721 showed that it does not possess promoter activity, and a RT-PCR analysis confirmed that the genes CC2720-CC2721 are co-transcribed and belong to the CC2720-CC2725 operon. The expression of the operon showed significant induction in the presence of Cd2+, moderate induction in the presence of Zn2+ and Co2+, and a slight induction in the presence of Ni2+. The expression of the CC2388-CC2390 operon is highly induced in the presence of nickel and cobalt, not induced by cadmium and moderately induced by zinc. The DCC2724 strain is not sensitive to zinc, cobalt or nickel. The DCC2390 strain is sensitive to cobalt, slightly sensitive to nickel and not sensitive to zinc, and both strains are sensitive to cadmium. A double mutant was constructed, as well as a complemented strain, and results suggest that these are two efflux systems with distinct metal responses.
Wienecke, Sarah Verfasser], and Dieter [Akademischer Betreuer] [Jahn. "Untersuchungen zur XylR-vermittelten Regulation des Xylose-Operons und phänotypischen Heterogenität in Bacillus megaterium / Sarah Wienecke ; Betreuer: Dieter Jahn." Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817759/34.
Повний текст джерелаChen, Chaw-Yuan. "Regulation, Evolution, and Properties of the ato Qperon and its Gene Products in Escherichia coli." Thesis, University of North Texas, 1993. https://digital.library.unt.edu/ark:/67531/metadc277592/.
Повний текст джерелаToukoki, Chadia. "MTSR is a Dual Regulator that Controls Virulence Genes and Metabolic Functions in Addition to Metal Homeostasis in Group A Streptococcus." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/biology_diss/66.
Повний текст джерелаKim, Jean. "Alternative regulation of the alginate algD operon by an activated AlgB in nonmucoid Pseudomonas aeruginosa is dependent on Sigma 54." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/108.
Повний текст джерелаKühne, Caroline Verfasser], and Petra [Akademischer Betreuer] [Dersch. "Gene regulation dynamics of the flagellar master regulatory operon flhDC in Salmonella enterica serovar Typhimurium / Caroline Kühne ; Betreuer: Petra Dersch." Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817678/34.
Повний текст джерелаMunevar, Nicolas Federico Villamil. "Análise do metabolismo de polifosfato e do operon pst em Pseudomonas aeruginosa." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-10122015-101302/.
Повний текст джерелаThe pst operon in P. aeruginosa encodes a high-affinity phosphate transporter and the PhoU protein, which together act as repressors of Pho regulon of this species. The PhoU activity is also related with polyphosphate (polyP) metabolism, since phoU null mutants have a large accumulation of the biopolymer. β-galactosidase assays allowed to confirm a change in the expression of ppk and ppx genes, in-volved in PolyP metabolism, in the phoU mutant. It was also evidenced that in the wild type strain, the ppk and ppx transcription does not respond to Pi or nitrogen starvation, and that these genes are highly expressed under conditions of normal growth. In addition, it was determined that ppk is co-transcribed with hemB, a gene involved in the synthesis of porphyrins, and they constitute therefore an operon. The pst operon was also examined. Was identified by northern blot the transcript of the first gene in the operon, pstS, which encodes a periplasmic protein. Also, a promoter was identified immediately upstream of phoU, the most distal gene in the operon, allowing its expression in normal conditions of bacterial growth. Finally, it was determined by EMSA that the two consensus sequences Pho box present in the pst operon are fully functional.
Barbosa, Rosicler Lazaro. "Analise funcional e estrutural da proteina BigR de Xylella fastidiosa envolvida na regulação do operon Xf0768-0764." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316599.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-11T01:25:29Z (GMT). No. of bitstreams: 1 Barbosa_RosiclerLazaro_D.pdf: 3769373 bytes, checksum: ba15f76eb9d63f64834d030ffed2482a (MD5) Previous issue date: 2008
Resumo: O gene XF0767 de Xylella fastidiosa está localizado num operon composto por mais quatro genes classificados como proteínas hipotéticas (XF0768-XF0767-XF0766-XF0765-XF0764). Este operon está conservado em uma série de bactérias associadas a plantas, incluindo Agrobacterium tumefaciens, Mezorhizobium loti e Sinorhizobium meliloti. A região upstream ao códon de iniciação deste operon possui seqüências canônicas correspondentes a sítios -35 e ¿10 de regiões promotoras reguladas por fatores sigma70. O objetivo deste trabalho foi caracterizar o sistema de regulação do operon pela proteína XF0767, nomeada BigR (biofilm growth-associated repressor), visando à compreensão deste sistema e sua importância para a bactéria. A proteína BigR se liga na seqüência repetida invertida (9-4-9) localizada na região ¿10 do promotor do operon XF0768-0764, denominada BigRbox. BigR inibe a atividade do operon reprimindo a expressão do gene repórter GFP em Escherichia coli, Agrobacterium tumefaciens e Xylella fastidiosa. Mutações no BigRbox dos promotores de Xylella e Agrobacterium afetam a ligação do repressor e abole a transcrição do gene repórter. Esses dados indicam, portanto, que BigR compete com a RNA polimerase pelo mesmo sítio de ligação ao DNA, revelando assim o mecanismo de regulação do operon. BigR apresenta similaridade com fatores transcricionais de bactérias contendo domínio HTH (helix-turn-helix) de ligação ao DNA. Apesar de BigR ter sido inicialmente classificada como uma proteína da família SmtB/ArsR, as quais regulam operons relacionados à resistência a metais, nossos dados indicam que BigR não atua como sensor de metal. A adição de cádmio, ferro e cobre na mistura de ligação causam uma aparente dissociação do complexo DNA/BigR, entretanto, estudos in vivo na presença de metais não evidenciaram nenhuma alteração na expressão do gene repórter. Mutantes deficientes em BigR também não apresentam diferença de crescimento na presença de metais. O gene XF0768 codifica uma proteína nomeada BLH (beta-lactamase-like hydrolase) por pertencer à superfamília das metalo-beta-lactamases. Dada a presença de BigR e BLH no operon de Xylella e Agrobacterium, a atividade do operon foi analisada em diferentes condições como crescimento das células repórter in planta, co-cultivo com bactérias endofíticas e deficiência nutricional. Entretanto, uma maior atividade do operon em Xylella e Agrobacterium foi detectada apenas na condição de biofilme. Células de Agrobacterium aderidas em raiz de tabaco também apresentaram maior expressão do gene repórter quando comparadas às células em suspensão. Mutantes de Agrobacterium deficientes em BigR (bigR-) apresentam maior expressão do gene repórter, confirmando que BigR age como o repressor transcricional do operon. A quantificação da formação de biofilme das células mutante e selvagem revelou uma maior densidade de biofilme no mutante bigR- em superfície de vidro e também maior quantidade de células aderidas em raiz de tabaco, indicando que o operon pode estar envolvido com o processo de adesão ou desenvolvimento do biofilme bacteriano. Do ponto de vista estrutural, foi mostrado que a proteína BigR truncada no N-terminal (?BigR) encontra-se estruturada na forma de trímero, diferindo do observado para as proteínas com domínio HTH que em geral formam dímeros e monômeros. BigR é estável termicamente, começando a perder estrutura à 74oC, mas retendo um sinal residual de a-hélice até 90oC. Tratamentos com altas concentrações de (NH4) SO 2 4 interferiram na ligação da proteína ao DNA alvo e no conteúdo de estrutura secundária sem alterar, contudo, sua estabilidade térmica. A purificação e cristalização da proteína ?BigR resultou na coleta de alguns conjuntos de dados da proteína nativa e derivada, através de soaking ou marcada com SeMet. Apesar dos dados obtidos serem de boa qualidade, até o momento, não foi possível a resolução da estrutura cristalográfica desta proteína
Abstract: The XF0767 gene from Xylella fastidiosa is located in an operon composed by a set of genes (XF0768-XF0767-XF0766-XF0765-XF0764) of unknown function. This operon is conserved in a number of plant-associated bacteria including Agrobacterium tumefaciens, Mezorhizobium loti e Sinorhizobium meliloti. The DNA region upstream of the operon has canonical sequences corresponding to ¿35 and ¿10 elements found in sigma70-regulated promoters. The aim of this work was to elucidate the biological function of the protein encoded by XF0767, named BigR (biofilm growth-associated repressor), as a transcriptional regulator and its importance to the bacteria. BigR binds to an inverted repeat sequence (9-4-9) located in the ¿10 region of the XF0768-0764 operon promoter. This sequence was named BigRbox. BigR repressed transcription of its own operon upon binding to the BigRbox in Xylella fastidiosa and Agrobacterium tumefaciens. Mutations in the BigRbox significantly affected the repressor binding and abolished transcription of the reporter gene in both bacteria, indicating that BigR compete with the RNA polymerase for the same promoter site. BigR is similar to HTH transcriptional factors of the SmtB/ArsR family, which control tolerance and detoxification of heavy metals in prokaryotes. Despite the similarities, BigR does not appear to function as a metal sensor, as initially predicted. Although binding of BigR to its target DNA was diminished in the presence of cadmium, copper and iron, operon regulation in response to metals was not demonstrated in vivo. In addition, Agrobacterium mutants deficient in BigR did not show changes in growth rates in the presence of metals. BLH is an unusual beta-lactamase-like hydrolase coded by the XF0768 gene. To gain insights into the possible function of the operon, the activity of reporter cells was observed in the presence of different compounds and conditions including in planta growth, effect of endophytic competition and nutrient deficiency. Significantly, an increased operon activity was observed in Xylella and Agrobacterium biofilms. Agrobacterium cells attached to tobacco roots also showed high levels of reporter gene expression in comparison to cells in suspension. A. tumefaciens mutants deficient in the BigR showed constitutive expression of the operon, confirming that BigR acts as a transcriptional repressor. Biofilm quantification showed increased biofilm formation in glass surfaces as well as in tobacco roots, indicating that the operon may play a role in cell adherence or biofilm development. Structurally, the truncated BigR protein (?BigR) is a trimmer in solution, as opposed to most HTH regulators known, which are usually found as dimmers or monomers. BigR is stable at high temperatures (74oC); however, treatments with high concentrations of ammonium sulfate interfere with the protein secondary structure and its DNA binding capacity, without affecting its thermal stability. Good quality X-ray diffraction data were collected for the native and derivative ?bigR protein; however, structure resolution was not possible probably due to problems with the crystals
Doutorado
Genetica de Microorganismos
Doutor em Genetica e Biologia Molecular