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1
Kiromah, Naelaz Zukhruf Wakhidatul, Sinta Wahyu Septiani, Wahyu Rahmatulloh, and Ari Purnomo Aji. "Penetapan Parameter Standar Simplisia dan Ekstrak Etanol Daun Ganitri (Elaeocarpus serratus L.)." PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) 17, no. 1 (July 31, 2020): 207. http://dx.doi.org/10.30595/pharmacy.v17i1.8833.
Повний текст джерелаАнотація:
Ceylon olive (Elaeocarpus serratus L.) leaves have shown many pharmacological effects. Standardization of crude drug and extract needs to be conducted to assure the quality of the said material and further to support the pharmacological, pharmacokinetics, and toxicity effects. Standardization of Ceylon olive leaf extract is carried out to determine the standard parameters of crude drug and ethanolic extract of Ceylon olive leaves. Determination of the quality standard of crude drug and extract includes specific and non-specific parameters. The extract was obtained from a 70% ethanol maceration method with a yield of 16.02%. Macroscopic observations showed crude drugs of Ceylon olive leaves occurred as green in color, lanceolate leaves, 2-12 mm stems, and jagged leaves not deep, black spots, 10-15 side leaf bones, 6-18 cm long and 2-6 cm wide. Organoleptic of ethanolic extract of Ceylon olive showed thick consistency, blackish-brown colored, with a distinctive odor and a bitter taste. The water-soluble extractive value in crude drugs and extract was 16.48 and 51.54%, while the ethanol-soluble extractive value was 23.17 and 53.67%, respectively. The extract contained tannins, flavonoids, terpenoids, and saponins. Moisture content in the crude drugs and extracts was 22.09 and 25.53%. Total ash content in crude drugs and extracts was 33.53 and 30.62%, while their acid-insoluble ash content was 15.94 and 10.06%, respectively. Loss on drying of the crude drugs and extracts was 20.52 and 23.84% respectively. As the standard parameters of crude drugs and ethanolic extract of Ceylon olive leaves are not yet included in any formal monograph in Indonesia, the values of parameters reported in this study should be used as the reference of the standard quality parameter for those materials.
2
Maslennikova, M. S., and N. L. Solovyova. "Development of Compositions and Technologies for Producing Tablets with Hydroxythyrozol-standardized Extract of Olive Leaves." Drug development & registration 10, no. 3 (August 28, 2021): 47–53. http://dx.doi.org/10.33380/2305-2066-2021-10-3-47-53.
Повний текст джерелаАнотація:
Introduction. The article presents the results of a study of the physicochemical properties of dry extract of olive leaves, standardized by hydroxythyrozol - a biologically active substance that is part of the chemical composition of aerial parts of the olive tree.Aim. The purpose of the study is to develop tablet Formulations containing dry extract of olive leaves and to standardize by hydroxythyrozol by reversed-phase-HPLC method on the main substancecontent.Materials and methods. Substance of dry extract of olive leaves, microcrystalline cellulose (Avicel® PH-112), Aerosil® (Aeroperl® 300 Pharma), Ludipress® (Ludipress®), lactose, potato starch, sodium salt of carboxymethyl starch, magnesium stearate, direct pressing, HPLC.Results and discussion. Studies of the physicochemical properties of dry extract of olive leaves have been carried out. Selected excipients for the study of tablettable masses. Formulations for further tabletting have been developed. A complex of studies of the obtained tablets was carried out according to Russian Pharmacopoeia XIV for compliance with quality indicators.Conclusion. The biological properties of dry extract of olive leaves are have been studied, indicating the prospect of developing drugs based on it. Formulations of tablets with dry extract of olive leaves have been developed and their quality indicators have been investigated.
3
Baptista, Ana, Beata Shymon, Beatriz Silva, Catarina Lopes, Marta Pereira, Sofia Brandão, and Yaidelin Manrique. "Antibacterial Finishing of Cotton Bed Sheets using Olive Leaves Extract." U.Porto Journal of Engineering 8, no. 5 (September 27, 2022): 31–40. http://dx.doi.org/10.24840/2183-6493_008.005_0005.
Повний текст джерелаАнотація:
The main goal of this project consisted of the conceptualization and development of a product related to the olive tree chemical features to monetize the wastes of the olive fruit exploitation. The most abundant by-product of the olive fruit harvest is the olive leaves, used as the raw material in this project. By following the steps of Chemical Product Design, it was decided to pursue the idea of creating antibacterial cotton bed sheets impregnated with olive leaves extract, mainly composed of oleuropein. The production process includes a pre-treatment of the olive leaves, oleuropein extraction, and supercritical impregnation. The estimated amount of leaves required is 39 g/m2 of cotton, considering a cotton fabric with 115 GSM.
4
Khanum, Fakhara, Tahir Zahoor, Muhammad Issa Khan, and Muhammad Asghar. "Biochemical Profile of Olive Leaves Grown in "Olive Valley" Pakistan." Biological Sciences - PJSIR 63, no. 1 (March 18, 2020): 9–16. http://dx.doi.org/10.52763/pjsir.biol.sci.63.1.2020.9.16.
Повний текст джерелаАнотація:
This study was designed to determine the best olive variety for cultivation in Pakistan. The leaves of eight locally grown varieties were analyzed for levels of moisture, total solids, ash, fat, fibre, protein, K, Ca, Mg, Na, Fe, Mn, Zn and Cu levels. Leaves were then subjected to solvent extraction with each of water ratio in, 75% ethanol, 75% methanol, 95% ethanol and 95% methanol. The extract yield was significantly highest for 75% ethanol extract of all varieties. Significantly highest polyphenol and flavonoid contents were examined for Gemlik leaves extracted with 75% ethanol. We recommend cultivation of Gemlik in Pakistan and extraction of bioactive compounds from olive leaves will be the most efficient with a binary solution of 75% ethanol in water. Further studies should be planned to assess antioxidant, antimicrobial and food preservation properties of olive leaf extract from locally grown varieties.
5
Bouallagui, Zouhaier, Asma Mahmoudi, Amina Maalej, Fatma Hadrich, Hiroko Isoda, and Sami Sayadi. "Contribution of Major Polyphenols to the Antioxidant Profile and Cytotoxic Activity of Olive Leaves." Anti-Cancer Agents in Medicinal Chemistry 19, no. 13 (December 11, 2019): 1651–57. http://dx.doi.org/10.2174/1871520619666190416101622.
Повний текст джерелаАнотація:
Aim: This study was designed to investigate the phytochemical profile and the cytotoxic activities of the eco-friendly extracts of olive leaves from Chemlali cultivar. Materials and Methods: The Phenolic composition of olive leaves extracts, the antioxidant activity and the cytotoxic effects against MCF-7 and HepG2 cells were determined. Results: Olive leaves extracts showed relevant total polyphenols contents. Oleuropein was the major detected phenolic compound reaching a concentration of 16.9 mg/ml. The antioxidant potential of the studied extracts varied from 23.7 to 46.5mM Trolox equivalents as revealed by DPPH and ABTS assays. Cytotoxicity experiments showed similar trends for both HepG2 and MCF-7 cells with the infusion extract being the most active. Conclusion: This study denotes that olive leaves may have great potential as endless bioresource of valuable bioactive compounds which may have a wide application.
6
Kiritsakis, Konstantinos, Athanasia M. Goula, Konstantinos G. Adamopoulos, and Dimitrios Gerasopoulos. "Valorization of Olive Leaves: Spray Drying of Olive Leaf Extract." Waste and Biomass Valorization 9, no. 4 (July 19, 2017): 619–33. http://dx.doi.org/10.1007/s12649-017-0023-x.
Повний текст джерела
7
Al-Baarri, A. N., F. P. Lestari, H. M. Wahda, Widayat, and A. M. Legowo. "Physicochemical properties of vacuum packaged snake fruits in the presence of olive leave extract." Food Research 4, S1 (February 26, 2020): 250–55. http://dx.doi.org/10.26656/fr.2017.4(s1).s37.
Повний текст джерелаАнотація:
This research was done to investigate the effectiveness of the presence of olive leaf extract in vacuum packaging in maintaining the physicochemical properties of fresh-cut snake fruit under ambient temperature. The snake fruits were vacuum packaged in the presence of 3% (v/w) olive leave extract (OLE) and stored in room temperature for 8 days of storage. The physicochemical properties including color changes, firmness, and total dissolved solid (TDS) were observed. The results indicated that addition olive leaves extract in vacuum packaging gave the best result by inhibiting color changes by declining in total color difference by 10.88, BI 5.08, absorbance 2.750 Ǻ declining in hardness by 14.61% and lowest TDS level. As an alternative method of storing fresh-cut snake fruit under ambient conditions, vacuum packaging containing olive leave extract can be used.
8
Rashed, Shimaa A., Tamannouha I. Saad, and Salama M. El-Darier. "Potential aptitude of four olive cultivars as anticancer and antioxidant agents: oleuropein content." Rendiconti Lincei. Scienze Fisiche e Naturali 33, no. 1 (February 28, 2022): 195–203. http://dx.doi.org/10.1007/s12210-022-01056-x.
Повний текст джерелаАнотація:
AbstractOlea europaea L. (olive, Oleaceae) constitutes a source of many bioactive compounds, which have recognized benefits for both human health and technological purposes. The present article was carried out to evaluate the biological activity of oleuropein (an ester of 2-(3,4-dihydroxyphenyl) ethanol (hydroxytyrosol) which has the oleosidic skeleton that is common to the secoiridoid glucosides of Oleaceae). It occurred in leaf extracts of the four olive cultivars (Chemlali, Manzanilla, Picaul and Toffahi) as a source for some anticancer and antioxidant agents and their consequences on the action of Hordeum vulgare (barley). The total phenolic and flavonoid compounds were extracted from olive leaves by ethanol 95% then analyzed by high-performance liquid chromatography (HPLC). The study evaluates the anticancer activity of the ethanolic extract of olive leaves against breast and hepatocellular carcinoma cells showing high values. Also, the extract exhibited highly consequence on the antioxidant potentiality of barley which was assessed using the diphenyl picryl hydrazyl method (DDPH). These results pave the way for utilization of olive leaves as a source of natural anticancer and antioxidant agents.
9
Himour, S., A. Yahia, and H. Belattar. "Oleuropein and Antibacterial Activities of Olea europaea L. Leaf Extract." European Scientific Journal, ESJ 13, no. 6 (February 28, 2017): 342. http://dx.doi.org/10.19044/esj.2017.v13n6p342.
Повний текст джерелаАнотація:
In this study, we reported the determination of phenolic compounds in olive leaves by reversed phase HPLC/DAD and the evaluation of their in vitro activity against several microorganisms. These organisms might however, be causal agents of human intestinal and respiratory tract infections. Extract of the leaves of two varieties of Olea europaea L. (Chemlel and Dathier) was investigated for antibacterial activity against four pathogenic bacteria. Leaves extract was prepared using water and methanol (20/80) in a cold extraction process. The tested bacteria were Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus .The extracts were found to be effective against all isolates tests. Ethanolic extract at a concentration of 100 % presented the highest potential of inhibiting variety of Dathier against S.aureus .This is with an inhibition zone of 17.49 mm and 15.66 mm for the variety Chemlel against S.aureus. The high Oleuropein content and the important antibacterial activities of olive leaves extract could be useful sources for industrial extraction and pharmacological application.
10
Bayram, Mustafa, Semra Topuz, and Cemal Kaya. "Zeytin Yaprağı Ekstraktı ve Oleuropeinin Antioksidan, Antimikrobiyal Aktivitesi, Gıdalarda Kullanım Olanakları." Turkish Journal of Agriculture - Food Science and Technology 8, no. 2 (February 27, 2020): 337. http://dx.doi.org/10.24925/turjaf.v8i2.337-347.3039.
Повний текст джерелаАнотація:
Olive leaves which is one of the by-products of olive tree cultivation and olive processing industry, have been used in traditional folk medicine for centuries. In recent studies, it has been determined that olive leaf has many bioactivities and these effects have been associated with high phenolic compound content. The most dominant phenolic compound of olive leaf is oleuropein, which is the heterosidic ester of elenolic acid and 3,4-dihydroxyphenylethanol. Therefore, some studies have been carried out for extracting high value added compounds from olive leaves in recent years. Antioxidant and antimicrobial activities of crude phenolic extract and oleuropein have been investigated. Moreover, some studies have been carried out to increase the possibility of using olive leaf extract and oleuropein in food industry due to increasing suspicion of side effects and toxicity of synthetic food preservatives. In this review, it was aimed to investigate phenolic compounds of olive leaf extract, phenolic compound extraction from olive leaf as well as antioxidant, antimicrobial activity of olive leaf extract and oleuropein and possibilities of use in foods.
11
Cuffaro, Doretta, Simone Bertini, Marco Macchia, and Maria Digiacomo. "Enhanced Nutraceutical Properties of Extra Virgin Olive Oil Extract by Olive Leaf Enrichment." Nutrients 15, no. 5 (February 21, 2023): 1073. http://dx.doi.org/10.3390/nu15051073.
Повний текст джерелаАнотація:
(1) Background: Nowadays, the health-promoting properties of extra virgin olive oil (EVOO), including the antioxidant and anti-inflammatory actions, are well recognized and mainly attributed to the different polyphenols, such as oleocanthal and oleacein. In EVOO production, olive leaves represent a high value by-product, showing a wide spectrum of beneficial effects due to the presence of polyphenols, especially oleuropein. Here we report the study of olive leaf extract (OLE)-enriched EVOO extracts, obtained by adding different percentages of OLE to EVOO in order to ameliorate their nutraceutical activities. (2) Methods: The polyphenolic content of the EVOO/OLE extracts was analyzed by HPLC and the Folin-Ciocalteau assay. For further biological testing, an 8% OLE-enriched EVOO extract was chosen. Therefore, antioxidant effects were evaluated by three different methods (DPPH, ABTS, and FRAP), and the anti-inflammatory properties were assessed in terms of cyclooxygenase activity inhibition. (3) Results: The antioxidant and anti-inflammatory profiles of the new EVOO/OLE extract are significantly improved compared to those of EVOO extract; (4) Conclusions: The combination of OLE and EVOO extract can lead to an extract enriched in terms of bioactive polyphenols and endowed with better biological properties than the singular EVOO extract. Therefore, it may represent a new complement in the nutraceutical field.
12
BEN MANSOUR-GUEDDES, Samia, Dhouha SAIDANA-NAIJA, Ameni BCHIR, and Mohamed BRAHAM. "Climate change effects on phytochemical compounds and antioxidant activity of Olea europaea." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 48, no. 1 (March 31, 2020): 436–55. http://dx.doi.org/10.15835/nbha48111615.
Повний текст джерелаАнотація:
To tolerate water shortage and high temperature, olive cv. ‘Meski’, the main variety of table olives in Tunisia, developed several biochemical changes. The hereby study focused on the adaptation of the olive tree to the climatic conditions, considering the evaluation of phenolic compounds, chlorophylls, carotenoids, saponin and steroid synthesis, as well as the evaluation of the antioxidant activity. The analyses were made upon fresh leaves collected from three coastal areas of Tunisia (North (sub-humid), center (higher semi-arid) and South (lower arid)) and using different leaves’ extracts. The results emphasized that Southern ‘Meski’ trees had leaves with more polyphenols, flavonoids, o-diphenols and tannins compared with the Northern ones. From the North to the South, ‘Meski’ leaves showed an increase of carotenoids and a decrease of chlorophyll a and b contents. The highest level of antioxidant compounds of Southern leaves could be contributed to reduce the oxidative stress of the olive tree. The spectrophotometric analysis of the antioxidant capacity of leaves collected from central and Southern areas, based on DPPH and ABTS radical-scavenging activity, showed a higher value of antioxidant activity than the Northern ones, at different extract concentrations. Therefore, the increase of the analyzed bioactive compounds can be considered as a response of the tree to surround aggressions and to oppose the oxidative stress that results from the severity of climatic conditions, characteristic of the Southern area.
13
Rashed, Khaled. "Phytocontent and Biological effects of Olea europaea: A Review." Plantae Scientia 5, no. 2 (April 9, 2022): 36–44. http://dx.doi.org/10.32439/ps.v5i2.36-44.
Повний текст джерелаАнотація:
Olive tree (Olea europaea L., Oleaceae) leaves have been widely used in traditional herbal medicine to prevent and treat various diseases especially in Mediterranean countries. They contain several potentially bioactive com-pounds that may have hypoglycemic and hypolipidemic properties. Olea europaea is commonly known as Zaitoon. The ancient Egyptians, Greeks, Romans and other Mediterranean nations cultivated olives for its edible fruits and to obtain oil from them. Leaves of the tree became important when olive leaf extract was reported to be potent in reducing incidence of degenerative diseases, particularly coronary heart disease (CHD) and cancers of the breast, skin, and colon. Olive leaves have the highest antioxidant power among the different parts of the olive tree. While in olive fruit, phenols (e.g. TYR) and terpenoid hydrocarbon (squalene) are the major components found in the oil. Much research has been carried out on the medical applications of olive. The choice of the plant was based on the good previous biological study of Olea europaea. Interest to choose this plant, may have been due to the widespread use of olive species medicinally, food industry and cosmetics.
14
Centrone, Mariangela, Mariagrazia D’Agostino, Graziana Difonzo, Alessandra De Bruno, Annarita Di Mise, Marianna Ranieri, Cinzia Montemurro, et al. "Antioxidant Efficacy of Olive By-Product Extracts in Human Colon HCT8 Cells." Foods 10, no. 1 (December 23, 2020): 11. http://dx.doi.org/10.3390/foods10010011.
Повний текст джерелаАнотація:
The production of olive oil is accompanied by the generation of a huge amount of waste and by-products including olive leaves, pomace, and wastewater. The latter represents a relevant environmental issue because they contain certain phytotoxic compounds that may need specific treatments before the expensive disposal. Therefore, reducing waste biomass and valorizing by-products would make olive oil production more sustainable. Here, we explore the biological actions of extracts deriving from olive by-products including olive pomace (OP), olive wastewater (OWW), and olive leaf (OLs) in human colorectal carcinoma HCT8 cells. Interestingly, with the same phenolic concentration, the extract obtained from the OWW showed higher antioxidant ability compared with the extracts derived from OP and OLs. These biological effects may be related to the differential phenolic composition of the extracts, as OWW extract contains the highest amount of hydroxytyrosol and tyrosol that are potent antioxidant compounds. Furthermore, OP extract that contains a higher level of vanillic acid than the other extracts displayed a cytotoxic action at the highest concentration. Together these findings revealed that phenols in the by-product extracts may interfere with signaling molecules that cross-link several intracellular pathways, raising the possibility to use them for beneficial health effects.
15
Abdullah, B. N., K. K. Khudiar, and B. S. Toma. "Diuretic effects of aqueous extract of Olive Leaves (Olea europea) in adult male rats." Iraqi Journal of Veterinary Medicine 27, no. 1 (June 28, 2003): 50–60. http://dx.doi.org/10.30539/ijvm.v27i1.1096.
Повний текст джерелаАнотація:
This study was carried out to investigate the effects of aqueous extract of olive leaves (Olea europea) on kidney functions. Eighteen adult male rats
three equal groups placed individually in were randomly divided in to metabolic cages and were handled as follows: group (C) served as control group; rats in group (T1) were intubated (90 mg/kg B.W.) of aqueous extract of
group T2 were intubated (0.8 mg/kg B.W.) of olive leaves and rats in ammuretic. Blood and urine samples were collected after 24 hours of
significant increase in urinary output intubation. The results showed a
ions and potassium urinary sodium increase in an
by accompanied concentration in the olive leaves treated group only. Creatinine clearance (as an
for glomerular filtration rate) increased significantly in both treated index groups, it is concluded that olive leaves extract may cause its diuretic effect by. increasing the glomerulor filtration rate.
16
Sara, Himour, Belhain Hayat, and Bouketta Mehbouba. "Anticoagulant Activities of Olea Europaea Leaves and Fruit Extract." European Scientific Journal, ESJ 13, no. 30 (October 31, 2017): 90. http://dx.doi.org/10.19044/esj.2017.v13n30p90.
Повний текст джерелаАнотація:
The objective of this study is the evaluation of the anticoagulant activity of polyphenols obtained from extracts of the leaves, and fruits of olive (Olea europaea L.) of four varieties cultivated in the station Maazouzi Lakhdar (Mila, Algeria). The anticoagulant activity of the polyphenolic extracts, of the leaves and fruits of olive have been evaluated in vitro, using the test of Quick time (QT). The values of clotting time a showed a surprising anticoagulant activity on the exogenous pathway of coagulation, significant in the case of leaves and fruits of the Chemlel variety.
17
Journal, Baghdad Science. "Study the Effects of Olive Leaves Extracts in the activity of the enzyme GOT and their Biological Activities." Baghdad Science Journal 14, no. 1 (March 5, 2017): 48–59. http://dx.doi.org/10.21123/bsj.14.1.48-59.
Повний текст джерелаАнотація:
The olive tree, has been used it is important plant for the time being some of their parts on a large scale in the treatment of gastrointestinal disorders and stimulate circulation . Moreover, it is used as antibacterial material and also to address some of the respiratory system, diabetes, food preservation osteoporosis. This study involved the collection of olive leaves from different areas in Baghdad / Iraq. These leaves have been harvested, wash it, then dried and crushed, where the study aimed to identify the active ingredients and chemical elements in the olive leaf as well as its effect on the action of GOT enzyme .The study showed that the aqueous extracts (cold and hot) of the olive leaves powder are acidic in nature pH values are of (5.74 and 5.40) for the aqueous extracts hot and cold respectively. Study revealed the extract contain the a collection of Glycosides, tannins, phenolic compounds, resins, flavonoids, alkaloids, terpenes and compound Alaolurobin. The study also showed ability to activate the enzyme GOT in cold aqueous extract (8.36%). and the percentage (27.35%) of hot aqueous extract. That can be analyzed to the presence of higher concentrations of the active compounds in hot aqueous extract compared with cold aqueous extract especially tannins working to activate enzymes carrier in the cell membrane in the body. The study showed that the analysis of thin layer chromatography, liquid high-performance, ( TLC, HPLC) in extract compounds of phenols olive leaf using a mixture (chloroform, acetic acid) and a 2:5 was more impact in the Gram-positive Staphylococcus aureus, while less impact in a negative Gram Salmonella typhimurium. As proved accurate analysis of the chemical elements in powder of plant leaf olive tree and at different concentrations contain of chemical elements Major estimated by (g /kg) and trace estimated by (mg/kg). Since the existence of these elements led to increasing of enzymatic effectiveness through an increase process of activity the enzyme (GOT), which plays role of important in our bodies being gives indication of the nature of the work and effectiveness of the activity of some members of the body (liver, kidney, pancreas, etc.).
18
Borjan, Dragana, Maja Leitgeb, Željko Knez, and Maša Knez Hrnčič. "Microbiological and Antioxidant Activity of Phenolic Compounds in Olive Leaf Extract." Molecules 25, no. 24 (December 15, 2020): 5946. http://dx.doi.org/10.3390/molecules25245946.
Повний текст джерелаАнотація:
According to many reports, phenolic compounds isolated from olive leaves have very good biological activities, especially antimicrobial. Presently, the resistance of microorganisms to antibiotics is greater than ever. Therefore, there are numerous recent papers about alternative solutions for inhibiting their influence on human health. Olive leaf is studied as an important source of antimicrobials with low cost and used in medicine. Numerous publications on involving green technologies for isolation of active compounds from olive leaves have appeared over the past few decades. The present review reports on current knowledge of the most isolated phenolic compounds from olive leaf extract as well as methods for their isolation and characterization. This paper uses recent research findings with a wide range of study models to describe the antimicrobial potential of phenolic compounds. It also describes the vast range of information about methods for determination of antimicrobial potential focusing on effects on different microbes. Additionally, it serves to highlight the role of olive leaf extract as an antioxidants and presents methods for determination of antioxidant potential. Furthermore, it provides an overview of presence of enzymes. The significance of olive leaves as industrial and agricultural waste is emphasized by means of explaining their availability, therapeutic and nutritional effects, and research conducted on this field.
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Badawi, Sally, Saleh Ahmed, and Nabeel Al -Ani. "Effect of Ethanolic Olive leaf and its Callus Ethanol Extracts in Alloxan- induced Diabetic mice (Blood glucose and lipid profiles)." Journal of Biotechnology Research Center 7, no. 2 (June 1, 2013): 63–69. http://dx.doi.org/10.24126/jobrc.2013.7.2.268.
Повний текст джерелаАнотація:
This study was designed to test the lipid-lowering and antidiabetic activities of olive leaf and its callus extract. Diabetes in mice was induced by intraperitoneal injections of alloxan. The serum glucose and serum lipid were examined. Diabetic mice showed hypeglycemia, hypelipidemia. The administration, for 2 weeks of olive leaf and its callus extracts significantly decreased the Total cholesterol (TC). Triglycerides (TG). Low density lipoprotein (LDL), very low density lipoprotein (VLDL). Both types of olive extracts had significant hypoglycemic effects on blood glucose levels in diabetic mice. This hypoglycemic effect was as potent as the hypoglycemic effect of insulin. However, the callus extract was more potent than the leaves extracts and most potent than insulin in causing a significant decrease in LDL, VLDL, TC, TG and in antidiabetic effects.
20
Alcántara, Cristina, Tihana Žugčić, Radhia Abdelkebir, Jose V. García-Pérez, Anet Režek Jambrak, José M. Lorenzo, María Carmen Collado, Daniel Granato, and Francisco J. Barba. "Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts." Molecules 25, no. 7 (April 9, 2020): 1718. http://dx.doi.org/10.3390/molecules25071718.
Повний текст джерелаАнотація:
Mediterranean plants, such as fig and olive leaves, are well-known to exert beneficial effects in humans because of the presence of a wide range of bioactive compounds. However, scarce information regarding the impact of extraction methods, such as ultrasound and types of solvents, on their profile of antioxidant and anti-inflammatory compounds is provided. In addition, no information is available on the effects of extraction methods and solvents on the inhibition of pathogenic bacteria or promoting probiotic growth. In this scenario, this study was aimed to study the effects of ultrasound-assisted extraction (UAE) and solvent on the phenolic profile (Triple TOF-LC-MS/MS), antioxidant and anti-inflammatory compounds of olive and fig leaves. Results showed that UAE extracted more carotenoids compared to conventional extraction, while the conventional extraction impacted on higher flavonoids (olive leaves) and total phenolics (fig leaves). The antioxidant capacity of aqueous extract of fig leaves was three times higher than the extract obtained with ethanol for conventional extraction and four times higher for UAE. In general terms, hydroethanolic extracts presented the highest bacterial growth inhibition, and showed the highest anti-inflammatory activity. In conclusion, these side streams can be used as sources of bioactive compounds for further development of high-added-value products.
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Werghemmi, W., S. Abou Fayssal, H. Mazouz, H. Hajjaj, and L. Hajji. "Olive and green tea leaves extract in Pleurotus ostreatus var. florida culture media: Effect on mycelial linear growth rate, diameter and growth induction index." IOP Conference Series: Earth and Environmental Science 1090, no. 1 (October 1, 2022): 012020. http://dx.doi.org/10.1088/1755-1315/1090/1/012020.
Повний текст джерелаАнотація:
Abstract The exponential disposal of agro-industrial wastes onto the environment has endangered all forms of life. The implementation of these wastes in mushroom production is an eco-friendly and promising solution. The effect of olive and tea leaves extracts represented in culture media treatments: potato dextrose agar (PDA) 80% + tea extract (TE) 20% (T2), PDA 80% + olive extract (OE) 20% (T3), PDA 80% + OE 10% + TE 10% (T4), PDA 70% + OE 20% + TE 10% (T5) on mycelial growth diameter (MGD) and linear growth rate (MLGR) of Pleurotus ostreatus var. florida was compared to the one of PDA used as control (T1) at 22, 25 and 28°C inoculation temperatures. Optimum MGD was observed at 28°C in T1 (day 2), while it was significantly increased by 1.1–1.4 folds (days 4, 6, 8) in culture media containing plant extracts nevertheless the inoculation temperature. MLGR was improved by 102%–145% in olive/tea culture media compared to PDA nevertheless the inoculation temperature. Optimum growth induction index (GII) was observed in T3 (22.2%) at 22°C, T5 (21.9%) at 25°C and T4 (18.2%) at 28°C. These findings suggest the combination of olive and tea leaves extracts in the production of Pleurotus ostreatus.
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Vidal, Alfonso M., Manuel Moya, Sonia Alcalá, Inmaculada Romero, and Francisco Espínola. "Enrichment of Refined Olive Oils with Phenolic Extracts of Olive Leaf and Exhausted Olive Pomace." Antioxidants 11, no. 2 (January 21, 2022): 204. http://dx.doi.org/10.3390/antiox11020204.
Повний текст джерелаАнотація:
Refined olive oils (ROOs) are commonly enriched with synthetic antioxidants. Antioxidant extracts obtained from natural products can be used to improve the stability of these oils. In this study, ROOs were enriched through the addition of phenolic extracts from olive leaves (OLs) and exhausted olive pomace (EOP). In addition to replacing synthetic antioxidants with natural ones, this results in the valorization of these olive-derived biomasses. The most suitable method for mixing and enriching refined oils was probe-type ultrasonication using lecithin as the emulsifier. Thereafter, the change in the content of antioxidant compounds and the antioxidant capacity of the oils at 25, 35, and 45 °C were studied over 28 and 50 days of storage. The experimental results were fitted using a pseudo-first-order kinetic model. The oxidative stability index of the ROO enriched with a 2 g/L OL extract (70 h) was higher than that of a commercial ROO (46.8 h). Moreover, the oxidative stability index of the refined olive pomace oil (ROPO) enriched with a 2 g/L EOP extract (44.1 h) was higher than that of a commercial ROPO (38.9 h). In addition, the oxidative stabilities and antioxidant capacities of the oils were significantly correlated.
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Cedola, Annamaria, Carmen Palermo, Diego Centonze, Matteo Alessandro Del Nobile, and Amalia Conte. "Characterization and Bio-Accessibility Evaluation of Olive Leaf Extract-Enriched “Taralli”." Foods 9, no. 9 (September 10, 2020): 1268. http://dx.doi.org/10.3390/foods9091268.
Повний текст джерелаАнотація:
Olive leaves are rich in many compounds precious for human health. Due to this property, the current study was aimed to valorize the extract from this by-product in a cereal-based food, very popular all around the world, the “taralli”. To this aim, ultrasound-assisted extraction was applied to dried olive leaves to obtain the extract, used as “taralli” ingredient, instead of white wine. The “taralli” with and without extract was subjected to in vitro digestion to assess the quantity of polyphenolic compounds released in the gastrointestinal tract to become available for absorption. Total content of phenols and flavonoids, as well as the antioxidant capacity, was measured on both cooked and uncooked samples, before and after digestion. In addition, High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) of the three most abundant polyphenols present in olive leaf extracts, such as oleuropein, hydroxytyrosol, and verbascoside, was carried out at the three stages of the digestion process. The results showed that the substitution of white wine with olive leaf extract increased the total content of polyphenols and flavonoids and the antioxidant capacity. Bio-accessibility of the main phenolic compounds demonstrated that oleuropein resisted slightly after gastric digestion but was almost completely degraded in the intestinal phase, while hydroxytyrosol and verbascoside were not resistant to the digestion process from the gastric phase.
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Albogami, Sarah, and Aziza Hassan. "Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models." Molecules 26, no. 13 (July 3, 2021): 4069. http://dx.doi.org/10.3390/molecules26134069.
Повний текст джерелаАнотація:
Cancer is one of the most serious public health issues worldwide, ranking second only to cardiovascular diseases as a cause of death. Numerous plant extracts have extraordinary health benefits and have been used for centuries to treat a variety of ailments with few side effects. Olive leaves have a long history of medicinal and therapeutic use. In this study, the anti-cancer properties of an olive leaf extract were investigated in vitro using colorectal and prostate cancer cell lines (HT29 and PC3, respectively). A high-performance liquid chromatography analysis showed that the olive leaf extract contained a high chlorogenic acid content. Accordingly, chlorogenic acid may be related to the observed effects of the aqueous extract on cancer cells, including increased inhibition of cancer cell growth, migration, DNA fragmentation, cell cycle arrest at the S phase, reactive oxygen species (ROS) production, and altered gene expression. The effects of the extracts were greater in HT29 than in PC3 cells. These results suggest that chlorogenic acid, the main constituent in the olive extract, is a promising new anti-cancer agent. Further analyses should focus on its in vivo effects on colorectal tumor models, both alone and in combination with established agents.
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Miloradovic, Zoran, Maja Gvozdenov, Djurdjica Jovovic, Nevena Mihailovic-Stanojevic, Milan Ivanov, Una Vajic, Danijela Karanovic, Sladjan Milanovic, and Jelica Grujic-Milanovic. "Effect of Olea europea L. leaf extract on haemodynamic status and lipid peroxidation in spontaneously hypertensive rats." Veterinarski glasnik 67, no. 5-6 (2013): 303–15. http://dx.doi.org/10.2298/vetgl1306303m.
Повний текст джерелаАнотація:
Hypertension is one of the main causes of cardiovascular disorders and since ancient times olive tree leaves have been used in its therapy. However the mechanisms of their atihypertensive effect have not been sufficiently explained yet. The main objective of our study was to investigate acute effect of olive tree leaves extract on haemodynamics and lipid peroxidation in rats with congenital hypertension under normal and blocked synthesis of nitric oxide. For the purpose of our research, there were used olive tree leaf extract EFLA? 943 as well as inhibitor of nitric oxide synthase enzyme L-NAME. Nitric oxide synthesis inhibition led to statistically significant increase of mean arterial pressure, reducing heart rate and cardiac output, increase of total vascular resistance and lipid peroxidation in plasma. Treatment by olive leaf extract led to decrease of mean arterial pressure, reducing the frequency and cardiac output, without change in lipid peroxidation. Olive leaf extract under blockade of nitric oxide led to decrease of mean arterial pressure, total peripheral resistance remained high, cardiac output low, and lipid peroxidation significantly increased. General conclusion is that olive leaf extract has a strong antihypertensive effect, decreases cardiac pre and after load and does not influence lipid peroxidation. Under blockade of nitric oxide synthesis, this extract keeps antihypertensive properties, but due to strong endothelial dysfunction, it is unable to regulate increased total peripheral resistance and marked lipid peroxidation.
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Ozkan, M., F. Z. Biber Muftuler, A. Yurt Kilcar, E. I. Medine, and P. Unak. "Isolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methods." ract 101, no. 9 (September 2013): 585–93. http://dx.doi.org/10.1524/ract.2013.2068.
Повний текст джерелаАнотація:
Summary It is known that medicinal plants like olive have biological activities due to their flavonoid content such as olueropein, tyrosol, hydroxytyrosol etc. In current study, hydroxytrosol (HT) which is one of the major phenolic compounds in olive, olive leaves and olive oil, was isolated after methanol extraction and purification of olive leaves which are grown in the northern Anatolia region of Turkey. The isolated HT was radiolabeled with 131I (131I-HT) and the bioaffinity of this radiolabeled component of olive leaves extract was investigated by using in vivo/in vitro methods. It was found that HT could be radiolabeled with 131I in yields of 95.6±4.4% (n = 8), and in vivo studies showed that 131I-HT is taken up by urinary bladder, stomach, small intestine, large intestine, breast and prostate. Significant incorporation of activity was observed in cell lines via in vitro studies.
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Hinad, Ibrahim, Youssef S’hih, Radia El Gui, Aboubaker Elhessni, Abdelhalim Mesfioui, and Moulay laarbi Ouahidi. "Acute and subacute toxicity study of the methanolic extract of olea europea.L leaves in Wistar rat." E3S Web of Conferences 319 (2021): 01094. http://dx.doi.org/10.1051/e3sconf/202131901094.
Повний текст джерелаАнотація:
Olea europea.L (olive tree) is a plant widely used in the world. Its leaves have an extensive use in traditional herbal medicine to prevent and/or treat several diseases especially in Mediterranean regions. Despite this, research into the study of its toxicity is very limited in the literature. For this, this study aims to study the acute and subacute toxicity of methanolic extract olive leaves in Wistar rats. To study acute toxicity, four dose levels (50,300,2000 and 5000 mg/kg body weight) were tested by single oral administration to Wistar rats. The subacute toxicity for 28 days was conducted by daily oral administration of methanolic extract of olive leaves at doses 1000,2000 and 3000 mg/kg in rats Wistar. After fourteen days of the administration of escalating doses, no deaths were recorded in the animals and no clinically detectable signs were observed. The results of subacute toxicity showed that the body weight of rats was not significantly changed by administration of methanolic extract of olive leaves. Monitoring of the behavior of the rats during the study showed the appearance of some manifestations of tremor, agitation and diarrhea in rats treated with the dose of 3000mg/kg. Grosse examination of the kidneys and liver revealed no changes in those organs and histopathological examination showed congestion and inflammation of the fat in rats treated with the dose of 3000mg/kg. the hematological profile showed no significant changes of the measured hematological parameters. Olive leaves should be used with care and caution since its methanolic extract caused some signs of toxicity and may affect the liver at repeated dose. however, more studies are needed to verify and clarify the toxic aspect of olea europea.L leaves.
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Burò, Ilaria, Valeria Consoli, Angela Castellano, Luca Vanella, and Valeria Sorrenti. "Beneficial Effects of Standardized Extracts from Wastes of Red Oranges and Olive Leaves." Antioxidants 11, no. 8 (July 30, 2022): 1496. http://dx.doi.org/10.3390/antiox11081496.
Повний текст джерелаАнотація:
The awareness of the large amount of waste produced along the food chain, starting in the agricultural sector and continuing across industrial transformation to the domestic context, has in recent years also aroused strong concern amongst the public, who are ing about the possible consequences that this could have on environmental sustainability, resource waste and human health. The aim of the present research is the recovery of substances with high added value from waste and by-products typical of the Mediterranean area, such as the residue from the industrial processing of red oranges, called pastazzo (peels, pulps and seeds), which is particularly rich in anthocyanins, flavanones and hydroxycinnamic acids, and has numerous nutraceutical properties, as well as the olive leaves coming from olive-tree pruning, which are rich in substances such as oleuropein, elenolic acid, hydroxytyrosol, tyrosol and rutin. The effect of Red Orange Extract (ROE) and Olive Leaf Extract (OLE) on HepG2 fatty storage capacity was assessed performing Oil Red O’ staining, and antioxidant properties of the extracts were evaluated following the steatosis model onset. Based on the results obtained, the preparation of natural extracts that are derived from these waste products can be useful for preventing, counteracting or delaying the onset of the complications of fatty liver disease, such as hepatic steatosis.
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El-Kholany, A. S., M. M. Abdelmegeed, M. A. Abd-Elraheem, M. S. Deraz, and M. A. Elshaer. "Chemical Evaluation and Biological Activity of Olive Leaves Extract." Al-Azhar Journal of Agricultural Research 47, no. 1 (June 1, 2022): 35–45. http://dx.doi.org/10.21608/ajar.2022.266482.
Повний текст джерела
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Kashaninejad, M., M. T. Sanz, B. Blanco, S. Beltrán, and S. Mehdi Niknam. "Freeze dried extract from olive leaves: Valorisation, extraction kinetics and extract characterization." Food and Bioproducts Processing 124 (November 2020): 196–207. http://dx.doi.org/10.1016/j.fbp.2020.08.015.
Повний текст джерела
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Xie, Pujun, Lorenzo Cecchi, Maria Bellumori, Diletta Balli, Lisa Giovannelli, Lixin Huang, and Nadia Mulinacci. "Phenolic Compounds and Triterpenes in Different Olive Tissues and Olive Oil By-Products, and Cytotoxicity on Human Colorectal Cancer Cells: The Case of Frantoio, Moraiolo and Leccino Cultivars (Olea europaea L.)." Foods 10, no. 11 (November 16, 2021): 2823. http://dx.doi.org/10.3390/foods10112823.
Повний текст джерелаАнотація:
Phenolic and triterpenoid compounds of the olive tree are recognized as having a key role in health promotion, thanks to their multiple protective actions in humans. To expand the source of these bioactive compounds, the phenolic and triterpenoid profiles of leaf, branch, destoned fruit, destoned pomace, shell, seed, and extra virgin olive oil from the Frantoio, Leccino, and Moraiolo olive cultivars were simultaneously characterized by HPLC-DAD-MS. Overall, 43 molecules were quantitated and expressed on the obtained dry extracts. Oleuropein was mainly concentrated in branches (82.72 g/kg), fruits (55.79 g/kg), leaves (36.71 g/kg), and shells (1.26 g/kg), verbascoside (4.88 g/kg) in pomace, and nüzhenide 11-methyl oleoside (90.91 g/kg) in seeds. Among triterpenoids, which were absent in shells, the highest amount of oleanolic acid was found in olive leaves (11.88 g/kg). HCT-116 colorectal cells were chosen to assess the cytotoxicity of the dry extract, using the phytocomplex from Frantoio, which was the richest in phenols and triterpenoids. The IC50 was also determined for 13 pure molecules (phenols and terpenoids) detected in the extracts. The greatest inhibition on the cell’s proliferation was induced by the branch dry extract (IC50 88.25 μg/mL) and by ursolic acid (IC50 24 μM). A dose-dependent relationship was observed for the tested extracts.
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Dounia MEZOUAR, Mohammed AISSAOUI, Amina BENMESSAOUD, and Farid Boucif LAHFA. "Phytochemical analysis and alpha-amylase inhibitory property of olive (Olea europaea L.) leaves extracts." Journal of Natural Product Research and Applications 1, no. 03 (April 10, 2022): 56–67. http://dx.doi.org/10.46325/jnpra.v1i03.28.
Повний текст джерелаАнотація:
Evaluation of medicinal plants for their antidiabetic activities has increased considerably around the world as well as in Algeria. Olea europaea, commonly called Zeytoune, is traditionally used by Tlemcen population in Algeria, for treating diabetes mellitus.
In this work, we evaluate the capacity of O. europaea leaves crude extracts to inhibit in vitro α-amylase activity.
The qualitative phytochemical screening carried out on O. europaea leaves extracts showed the presence of tannins, sterols and triterpenes, saponins, flavonoids and terpenoids. A quantitative analysis of the crude extracts showed significant levels of total polyphenols and flavonoids in hydroacetonic extract, with an amount of 802.57 ± 0.001 mg GAE/ g and 359 ± 0.002 mg CE/ g, respectively. In vitro tests carried out on the inhibitory of α-amylase activity, revealed an inhibitory effects, specifically with hydroacetonic extract in a concentration-dependent manner, with an IC50 value of 0.27 ± 0.02 mg/mL. These findings reveals that O. europaea leaves extracts could represent an interesting source of bioactive compounds and allow to the development of new antidiabetic agents.
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Monteleone, Julieta Ines, Elisa Sperlinga, Laura Siracusa, Giovanni Spagna, Lucia Parafati, Aldo Todaro, and Rosa Palmeri. "Water as a Solvent of Election for Obtaining Oleuropein-Rich Extracts from Olive (Olea europaea) Leaves." Agronomy 11, no. 3 (March 3, 2021): 465. http://dx.doi.org/10.3390/agronomy11030465.
Повний текст джерелаАнотація:
Leaves from Olea europaea represent one of the main by-products of the olive oil industry, containing a plethora of bioactive compounds with several promising activities for human health. An organic solvent-free extraction method was developed for the recovery of olive leaf phenols, which obtained an extract containing oleuropein in high amounts. A comparison of various extraction media is reported, together with the total phenolic content, DPPH (2,2-Diphenyl-1-picrylhydrazyl) content, ORAC (oxygen radical absorbance capacity), and polyphenol oxidase activity of the corresponding extracts. The polyphenol profiles and content of the most representative extracts have also been studied. Extraction solvent and temperature significantly influenced the phenolic content and antioxidant activity of the extracts, with hot water representing the solvent of election for the extraction of bioactive compounds from this matrix. All the extracts obtained showed reasonably high total phenol content (TPC) and good DPPH radical scavenging activity; among them, the water extract is characterized by desirable traits and could be used for many industrial applications and human consumption.
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Al-taee, E. H. "EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 51, no. 5 (October 31, 2020): 1448–57. http://dx.doi.org/10.36103/ijas.v51i5.1155.
Повний текст джерелаАнотація:
This study was conducted to examine the possible effects of Ag-NPs synthesized using the olive leaf extract on histopathology and cytogenetic effect in mice. A total of thirty albino mice aged two months were divided into five treatment groups as follows: Group 1: served as control was administrated orally with 0.3 mg/kg bw of normal saline; Group 2 and 3 were administrated orally with varying doses of Ag-NPs synthesized on olive leaf extract (10 and 100 mg/kg bw, respectively) for 30 days; Group 4 and 5 were administrated orally with olive leaf crude extract (300 and 1000 mg/kg bw, respectively) for 30 days. At the end of the experimental period, the detection of possible chromosomal aberrations in blood samples and histopathology (liver, spleen, kidney, uterus, and testes) were carried out. Statistically significant differences (P<0.05) were observed for the chromosomal aberrations in all Ag-NPs groups compared to control and to crude olive leaf extract groups. Histopathological study revealed various alterations in internal organs at high doses of Ag-NPs group including inflammatory reaction, blood congestion, degeneration, fibrosis, mononuclear cell lesion, and necrosis. Slight changes were identified at both doses of crude olive extract treated groups. Based on these results, oral administration of Ag-NPs could cause genotoxic and inflammatory responses in mice and this could be representing a risk to both environment and human health.
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Cádiz-Gurrea, María de la Luz, Diana Pinto, Cristina Delerue-Matos, and Francisca Rodrigues. "Olive Fruit and Leaf Wastes as Bioactive Ingredients for Cosmetics—A Preliminary Study." Antioxidants 10, no. 2 (February 5, 2021): 245. http://dx.doi.org/10.3390/antiox10020245.
Повний текст джерелаАнотація:
Olea europaea cultivar, native in the Mediterranean basin, has expanded worldwide, mainly due to the olive oil industry. This expansion is attributed to the benefits of olive oil consumption, since this product is rich in nutritional and bioactive compounds. However, the olive industry generates high amounts of wastes, which could be related to polluting effects on soil and water. To minimize the environmental impact, different strategies of revalorization have been proposed. In this sense, the aim of this work was to develop high cosmetic value added oleuropein-enriched extracts (O20 and O30), a bioactive compound from olive byproducts, performing a comprehensive characterization using high performance liquid chromatography coupled to mass spectrometry and evaluate their bioactivity by in vitro assays. A total of 49 compounds were detected, with oleuropein and its derivatives widely found in O30 extract, whereas iridoids were mainly detected in O20 extract. Moreover, 10 compounds were detected for the first time in olive leaves. Both extracts demonstrated strong antioxidant and antiradical activities, although O30 showed higher values. In addition, radical oxygen and nitrogen species scavenging and enzyme inhibition values were higher in O30, with the exception of HOCl and hyaluronidase inhibition assays. Regarding cell viability, olive byproduct extracts did not lead to a decrease in keratinocytes viability until 100 µg/mL. All data reported by the present study reflect the potential of industrial byproducts as cosmetic ingredients.
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Agatonovic-Kustrin, Snezana, Vladimir Gegechkori, Dementyev Sergey Petrovich, Kobakhidze Tamara Ilinichna, and David William Morton. "HPTLC and FTIR Fingerprinting of Olive Leaves Extracts and ATR-FTIR Characterisation of Major Flavonoids and Polyphenolics." Molecules 26, no. 22 (November 16, 2021): 6892. http://dx.doi.org/10.3390/molecules26226892.
Повний текст джерелаАнотація:
The aim of this study was to analyse the effect of spontaneous microbial maceration on the release and extraction of the flavonoids and phenolics from olive leaves. Bioprofiling based on thin-layer chromatography effect-directed detection followed by ATR-FTIR spectroscopy proved to be a reliable and convenient method for simultaneous comparison of the extracts. Results show that fermentation significantly enhances the extraction of phenolic compounds and flavonoids. The polyphenolic content was increased from 6.7 µg GAE (gallic acid equivalents) to 25.5 µg GAE, antioxidants from 10.3 µg GAE to 25.3 µg GAE, and flavonoid content from 42 µg RE (rutin equivalents) to 238 µg RE per 20 µL of extract. Increased antioxidant activity of fermented ethyl acetate extracts was attributed to the higher concentration of extracted flavonoids and phenolic terpenoids, while increased antioxidant activity in fermented ethanol extract was due to increased extraction of flavonoids as extraction of phenolic compounds was not improved. Lactic acid that is released during fermentation and glycine present in the olive leaves form a natural deep eutectic solvent (NADES) with significantly increased solubility for flavonoids.
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Czerwińska, Monika E., Katarzyna Duszak, Andrzej Parzonko, and Anna K. Kiss. "Chemical Composition and UVA-Protecting Activity of Extracts from Ligustrum vulgare and Olea europaea Leaves." Acta Biologica Cracoviensia s. Botanica 58, no. 2 (December 1, 2016): 45–55. http://dx.doi.org/10.1515/abcsb-2016-0016.
Повний текст джерелаАнотація:
Abstract Plants belonging to the family Oleaceae have been traditionally used in treatment of various inflammatory skin disorders. One of the most well-known species is Olea europaea L. (olive), cultivated in the Mediterranean countries. Another species is Ligustrum vulgare L. (common privet), occurring particularly in Northern Europe and Asia. The aim of the study was a comparison of the composition of aqueous and ethanolic extracts from leaves of O. europaea and L. vulgare (HPLC-DAD-MS), and determination of the total content of phenolics and flavonoids, as well as the content of the major compound, oleuropein. Secondly, we aimed to study the protective effect of extracts on reactive oxygen species (ROS) production by human fibroblast cells (NHDF), cell viability (MTT assay), and apoptosis rate (Annexin V/propidium iodide staining) after UVA-irradiation. The phytochemical analysis allowed us to identify compounds belonging to the groups of flavonoids, phenylpropanoids and secoiridoids in the extracts. The compounds from the group of lignans (olivil) were identified as being unique to O. europaea extracts. Echinacoside, ligustroflavone and ligustaloside A were identified in L. vulgare extracts in contrast to olive preparations. It was established that the aqueous and ethanolic extracts from leaves of both species, except the privet aqueous extract at a concentration of 5 μg/ml, did not show any significant inhibition of ROS production after UVA-irradiation in the model of NHDF cell line. The aqueous extracts of both species at concentrations of 5 and 25 μg/ml had a protective effect on the viability of UVA-treated cells in contrast to the ethanolic extract. In conclusion, no significant difference in the activity of olive and privet leaf extracts has been observed, which suggests that both plant materials’ extracts, particularly aqueous ones, are effective herbal medicines and photoprotectors, which – to some extent – confirms the use of their preparations in skin disorders.
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Debib, Aicha, and Mohamed Nadjib Boukhatem. "Phenolic Content, Antioxidant and Antimicrobial Activities of “Chemlali” Olive Leaf (Olea europaea L.) Extracts." International Journal of Pharmacology, Phytochemistry and Ethnomedicine 6 (January 2017): 38–46. http://dx.doi.org/10.18052/www.scipress.com/ijppe.6.38.
Повний текст джерелаАнотація:
In last decade, there is an increasing interest in researches for production of biologically active compounds from natural sources.OleaeuropaeaL. is used in traditional medicine in the Mediterranean areas. The aim of the current study was to investigate the content of phenol compounds and flavonoids extracted from "Chemlali" olive leaf collected from Mascara region (west of Algeria) followed by the assessment ofin vitroantioxidant and antimicrobial activities of the phenolic extracts against a wide spectrum of resistant human pathogens. Extraction was conducted at room temperature using four solvents: deionised water (ddH2O), 80% methanol (MeOH), and petroleum ether. Total phenols and total flavonoids were measured using the Folin-Ciocalteau and aluminium chloride colorimetric methods, respectively. The antioxidant properties have been determined by DPPH test. Results showed that the total phenol and flavonoid contents of the olive leaf extracts ranged from 3.64 in petroleum ether extract to 21.47 ± 0,05 mg gallic acid equivalents (GAE)/ g dried matter and from 3.33mg ± 0.07 to 17.64mg ± 0.07mg catechin equivalents (CE)/ g dried matter, respectively. Furthermore, our results revealed that extracting solvents have a significant influence on the antioxidant and antimicrobial properties. The three extracts possessed antibacterial activity against tested Gram-positive and Gram-negative bacteria particularly petroleum ether extract. The extract antimicrobial activity may be due to the presence of secoiridoid class. In conclusion, the data obtained in this study confirming the traditional use of this plant in treatment of infectious diseases. Phenolic compounds in olive leaves are major contributors to the antioxidant and antimicrobial effects of olive leaves. However, further detailed studies are required to determine the active ingredients responsible for these effects and to determine the mechanism of action of these compounds in the anti-microbial activity.
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Sekandi, Peter, Jane Namukobe, Jane Namukobe, Robert Byamukama, Hoseah M. Akala, Redemptah A. Yeda, and Matthias Heydenreich. "Bioactive secondary metabolites from the leaves of Secamone africana (Olive.) Bullock." International Journal of Biological and Chemical Sciences 14, no. 5 (September 14, 2020): 1820–30. http://dx.doi.org/10.4314/ijbcs.v14i5.25.
Повний текст джерелаАнотація:
Secamone africana leaves are used in the treatment of malaria and other ailments in Uganda. The aim of the study was to characterize the antiplasmodial compounds from the leaves of Secamone africana. The leaves were extracted sequentially using dichloromethane (DCM) and methanol (MeOH). The crude extracts and isolated compounds were evaluated for their antiplasmodial activity against the chloroquine sensitive Sierraleone I (D6) and chloroquine-resistant Indochina I (W2) strains of Plasmodium falciparum. Isolation and purification were done using chromatographic techniques including column chromatography and high performance liquid chromatography. The isolated compounds were characterized using spectroscopic methods. The MeOH extract (IC50 = 5.45 μg/mL) was found to be more active than the DCM extract (IC50= 15.93 μg/mL) against the D6 malaria parasite. Chemical investigation of the MeOH extract yielded one new compound; 2-(2,4-dimethyloxetan-2-yl) acetic acid (3) in addition to the six known compounds; α-linolenic acid (1), conduritol B (4), β-sitosterol (5), 3,4-dihydroxybenzoic acid (6), 4-hydroxybenzoic acid (7) and coumaric acid (8). The DCM extract yielded one known compound: 1-methyl cyclobutene (2). The presence of these compounds with good anti-plasmodial activities and other bioactivities reported in literature, appears to argue for the therapeutic potential of Secamone africana.Keywords: Secamone africana,, anti-plasmodial activity, chromatography, secondary metabolites.
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Hadrich, Fatma, Asma Mahmoudi, Mohamed Chamkha, Hiroko Isoda, and Sami Sayadi. "Olive Leaves Extract and Oleuropein Improve Insulin Sensitivity in 3T3-L1 Cells and in High-Fat Diet-Treated Rats via PI3K/AkT Signaling Pathway." Oxidative Medicine and Cellular Longevity 2023 (January 7, 2023): 1–14. http://dx.doi.org/10.1155/2023/6828230.
Повний текст джерелаАнотація:
Olive leaves extracts are known to exert potential pharmacological activities especially, antidiabetic and antiobesity. This study explores the anti-insulin resistant effect of olive leaves extracts and oleuropein in 3 T3-L1 cells and in high-fat diet fed rats. Our results showed that ethanol extract (EE) suppressed significantly ( P < 0.01 ) triacylglycerol accumulation. In preadipocytes cells, EE 1/100 decreased cell viability and induced apoptosis. Real-time PCR analysis showed that EE reduced the mRNA levels of adipogenesis (CEBP-α, PPARγ, SREBP-1c, and FAS) and proinflammatory (TNF-α and IL-6) genes. Moreover, the cotreatment of EE 1/1000 or oleuropein with insulin increased considerably the expression of p-IRS, p85-pI3K, and p-AKT. In vivo model, the oral administration of oleuropein at 50 mg/kg in rats fed with high fat diet for 8 weeks reduced inflammation in liver and adipose tissues (WAT), improved glucose intolerance, and decreased hyperinsulinemia. Furthermore, the immunohistochemistry revealed that the expression level of p-Akt, IRS1, and Glut-4 were significantly enhanced in liver and WAT tissues after oleuropein supplementation comparing with that in HFD group. Additionally, the expression of IRS1 was markedly ameliorated in pancreas. Our obtained results can be adopted as an approach to used olive leaves as complement to prevent insulin-resistance disease.
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Khizrieva, S. S., S. N. Borisenko, E. V. Maksimenko, and N. I. Borisenko. "Evaluation of different extraction techniques for the assay of anti - acetylcholinesterase activity of olive leaves (<i>Olea europaea</i>)." Chimica Techno Acta 8, no. 4 (November 9, 2021): 20218403. http://dx.doi.org/10.15826/chimtech.2021.8.4.03.
Повний текст джерелаАнотація:
The total phenol content and the anti-acetylcholinesterase activity were compared in olive leaf (OL) extracts obtained using both subcritical water extraction (SbWE) and conventional solvent extraction (ethanol-water). The method proposed by Ellman (in vitro) was used to study the inhibitory activity of acetylcholinesterase (AChE). The total content of phenolic compounds and AChE activities of OL extracts varied depending on the used extraction method. Thus, the extract obtained using the subcritical water technique (220 °C) showed the highest amounts of total phenolic components, expressed as gallic acid equivalents, (70.4 mg/g raw material) and the highest inhibitory AChE-activity (IC50 = 0.35 mg/ml). The obtained values of the anti-AChE activity of the extracts of OL demonstrated that the inhibitory activity for SbW-extract 120 °C (IC50 = 2.92 mg/ml) and SbW-extract 180 °C (IC50 = 0.8 mg/ml) is higher than that of the traditional extract (IC50 = 3.6 mg/ml), respectively. These results indicate a great potential of the subcritical water technique to develop the techniques to produce commercial extracts of OL, and these results could encourage improved utilization of the OL. The collected data on the anti - acetylcholinesterase activity of olive leaves clearly demonstrate the prospects for use of OL extracts in the development of novel pharmaceutical substances and nutraceuticals for the prevention and/or the treatment of Alzheimer's disease as well as some other neurodegenerative diseases.
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Acar-Tek, Nilüfer, and Duygu Ağagündüz. "Olive Leaf (Olea europaea L. folium): Potential Effects on Glycemia and Lipidemia." Annals of Nutrition and Metabolism 76, no. 1 (2020): 10–15. http://dx.doi.org/10.1159/000505508.
Повний текст джерелаАнотація:
Background: Olive tree (Olea europaea, Oleaceae) leaves have been widely used in traditional herbal medicine to prevent and treat various diseases especially in Mediterranean countries. They contain several potentially bioactive compounds that may have hypoglycemic and hypolipidemic properties. Summary: The literature has recently been attempting to define the relationship between olive leaf (Olea europaea L. folium) polyphenols and a number of health problems. Oleuropein, the basic phenolic compound of olive leaf and its extract, is responsible for the characteristic bitter taste and unique aroma of olive fruits. Furthermore, it is shown that oleuropein and its hydrolyzed products have many beneficial effects on human health because of its antioxidant characters. A number of studies report that olive leaf has potentially positive effects on the parameters related to diabetes and cardiovascular diseases by various mechanisms. Besides, toxicity studies suggest that olive leaf is generally safe even at high doses. Key messages: Although current results obtained until today seem promising, the studies in this subject are usually on cell culture and animal trials. Moreover, mostly the extract forms of olive leaves are used in the studies. More randomized controlled human clinical trials with extensive toxicity studies are needed to evaluate potential health effects and safety.
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El-sayed, Nazly R., Reham Samir, Lina Jamil M. Abdel-Hafez, and Mohammed A. Ramadan. "Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa." Antibiotics 9, no. 9 (August 19, 2020): 526. http://dx.doi.org/10.3390/antibiotics9090526.
Повний текст джерелаАнотація:
Biofilm acts as a complex barrier against antibiotics. In this study, we investigated the inhibitory activities of Olea europaea (olive) leaves Camellia sinensis (green tea), Styrax benzoin, Ocimum basilicum, Humulus lupulus, Ruta graveolens, and Propolis extracts on the biofilm formation, pyocyanin production, and twitching motility of Pseudomonas aeruginosa isolates. Moreover, we investigated the effect of olive leaf extract on the transcription of some biofilm related genes. A total of 204 isolates of Pseudomonas were collected from different Egyptian hospitals. A susceptibility test, carried out using the disc diffusion method, revealed that 49% of the isolates were multidrug-resistant. More than 90% of the isolates were biofilm-forming, of which 26% were strong biofilm producers. At subinhibitory concentrations, green tea and olive leaf extracts had the highest biofilm inhibitory effects with 84.8% and 82.2%, respectively. The expression levels of lasI, lasR, rhlI, and rhlR treated with these extracts were significantly reduced (p < 0.05) by around 97–99% compared to untreated isolates. This study suggests the ability of olive leaf extract to reduce the biofilm formation and virulence factor production of P. aeruginosa through the down regulation of quorum sensing (QS) genes. This may help in reducing our dependence on antibiotics and to handle biofilm-related infections of opportunistic pathogens more efficiently.
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Wang, Bixia, Shian Shen, Jipeng Qu, Zhou Xu, Shiling Feng, Tao Chen, and Chunbang Ding. "Optimizing Total Phenolic and Oleuropein of Chinese Olive (Olea europaea) Leaves for Enhancement of the Phenols Content and Antioxidant Activity." Agronomy 11, no. 4 (April 4, 2021): 686. http://dx.doi.org/10.3390/agronomy11040686.
Повний текст джерелаАнотація:
China, as a contrary climate to the Mediterranean-climate region, is massive in planting olive (Olea europaea) cultivation as an important oil crop. Nonetheless, Chinese olive leaves have received little attention and there is little information about the phenols content. Therefore, in this study, a multiple-response optimization was performed to maximize the total phenolic and oleuropein content (TPC and OEC) by ultrasound-assisted extraction, and the aged and young leaves prepared from six Chinese cultivars from January to December were investigated concerning seven main phenolic composition. Under optimal conditions (power 260 W, time 10 min, liquid-solid ratio 30 mL/g, and 50% ethanol), the highest TPC (197.32 mg/g DM) and OEC (74.68 mg/g DM) were obtained. Findings revealed that the optimal olive leaves for phenolic compounds were the young leaves in spring and winter, being oleuropein and luteolin-4′-O-glucoside present in higher level, and the “Koroneiki” and “Jiufeng” cultivars were relatively stable. Furthermore, antioxidant potential of the phenol extract and oleuropein was also evaluated based on the reducing power and scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH)and superoxide radical assays, and a higher antioxidant effect of oleuropein was observed compared to the phenol extract. In addition, oleuropein showed anticancer activity against HeLa cells, with a minimum inhibitory concentration (EC50) value of 0.19 mg/mL at 48 h. These findings revealed an attractive source of biological substances for further development and utilization of Chinese olive leaves.
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Orak, Hakime Hülya, Magdalena Karamać, Ryszard Amarowicz, Adnan Orak, and Kamila Penkacik. "Genotype-Related Differences in the Phenolic Compound Profile and Antioxidant Activity of Extracts from Olive (Olea europaea L.) Leaves." Molecules 24, no. 6 (March 21, 2019): 1130. http://dx.doi.org/10.3390/molecules24061130.
Повний текст джерелаАнотація:
The phenolic compound contents and antioxidant activities of the leaf extracts of nine olive genotypes were determined, and the obtained data were analysed using chemometric techniques. In the crude extracts, 12 compounds belonging to the secoiridoids, phenylethanoids, and flavonoids were identified. Oleuropein was the primary component for all genotypes, exhibiting a content of 21.0 to 98.0 mg/g extract. Hydroxytyrosol, verbascoside, luteolin 7-O-glucoside, and luteolin 4′-O-glucoside were also present in noticeable quantities. Genotypes differed to the greatest extent in the content of verbascoside (0.45–21.07 mg/g extract). The content of hydroxytyrosol ranged from 1.33 to 4.03 mg/g extract, and the aforementioned luteolin glucosides were present at 1.58–8.67 mg/g extract. The total phenolic content (TPC), DPPH• and ABTS•+ scavenging activities, ferric reducing antioxidant power (FRAP), and ability to inhibit the oxidation of -carotene-linoleic acid emulsion also varied significantly among genotypes. A hierarchical cluster analysis enabled the division of genotypes into three clusters with similarity above 60% in each group. GGE biplot analysis showed olive genotypes variability with respect to phenolic compound contents and antioxidant activities. Significant correlations among TPC, FRAP, the values of both radical scavenging assays, and the content of oleuropein were found. The contents of 7-O-glucoside and 4′-O-glucoside correlated with TPC, TEAC, FRAP, and the results of the emulsion oxidation assay.
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Antarsih, Novita Rina, Lutfi Hardiyanto, and Sri Widia A. Jusman. "Effect of Lamun Leaves [Enhalus acoroides (L.f.) Royle] on Leydig Cell Morphology." ENDLESS : International Journal of Future Studies 4, no. 1 (January 11, 2021): 8–14. http://dx.doi.org/10.54783/endless.v4i1.35.
Повний текст джерелаАнотація:
The ageing male process lead deterioration fertilization ability that caused of Leydig cell degeneration by an accumulation of oxidative stress with decreased testosterone levels. Enhalus acoroides contains antioxidants and phytosterols which can be converted into testosterone. The aim of this study to investigate the extract of Enhalus acoroides effect on Leydig cell morphology in male mice. Male mice was divided into 8 groups: adult mice no treatment (AG1), adult mice with olive oil (AG2), adult mice with extract of Enhalus acoroides at dose 25 mgKg-1 (AG3), adult mice with extract of Enhalus acoroides at dose 50 mgKg-1 (AG4), old mice no treatment (OG1), old mice with olive oil (OG2), old mice with extract of Enhalus acoroides at dose 25 mg Kg-1 (OG3), and at dose 50 mgKg-1 (OG4). All groups were repeated for 14 days. There was a significantly increased number of old Leydig cells in old mice group and adult mice group at dose 25 mgKg-1 compared without treatment. There is no difference in the observation of adult Leydig cell degeneration.
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Safarzadeh Markhali, Fereshteh, José A. Teixeira, and Cristina M. R. Rocha. "Effect of Ohmic Heating on the Extraction Yield, Polyphenol Content and Antioxidant Activity of Olive Mill Leaves." Clean Technologies 4, no. 2 (June 1, 2022): 512–28. http://dx.doi.org/10.3390/cleantechnol4020031.
Повний текст джерелаАнотація:
This study examined the influence of ohmic heating (OH), compared to the conventional heating (Conven) and Control (solvent) methods, on the extraction of olive mill leaves. The main extraction parameters were: (i) solvent ratio (aqueous ethanol; 40%, 60%, and 80%, v/v), and (ii) extraction temperature; 45 °C, 55 °C, and 75 °C (for OH and Conven), and room temperature (for Control). The selected response variables were extraction yield (%), total phenolic content (TPC), and antioxidant activity (ABTS and DPPH). The ohmic system, compared to Conven and Control, exhibited the greatest effects (p < 0.001) on increasing (i) extraction yield (34.53%) at 75 °C with 80% ethanol, (ii) TPC at 55 °C (42.53, 34.35, 31.63 mg GAE/g extract, with 60%, 40%, and 80% ethanol, respectively), and (iii) antioxidant potency at 75 °C detected by DPPH and ABTS, in the range of 1.21–1.04 mM TE/g, and 0.62–0.48 mM TE/g extract, respectively. Further, there were relatively similar trends in TPC and antioxidant activity (both methods), regardless of solvent ratios, p < 0.001. These findings demonstrate the potential of ohmic heating, as a green processing tool, for efficient extraction (15 min) of olive leaves. To date, no literature has described ohmic application for olive leave extraction.
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Khaleel, Rana I., Ahmad Al-Samarrai, Abdul-Hameed Hamoody, and Ghassan Al-Samarai. "Evaluation of the Efficacy of Some Plant Extracts as an Alternative to Chemical Weedicides in Controlling on Cyperus rotundus L." Al-Kitab Journal for Pure Sciences 2, no. 2 (December 30, 2018): 305–14. http://dx.doi.org/10.32441/kjps.02.02.p21.
Повний текст джерелаАнотація:
Two experiments were carried out during the summer season, 2016, in the green house / Department of Biology/Collage of Education, Samarra University, to evaluate the activity of three aqueous extract of leaves of Nerium (Nerium oleander L.),olives (Olea europaea .L) and castor (Ricinus communis.L), on control of tuber germination and growth of seedling of Cyperus rotundus L as an alternative for chemical pesticides. Experiments applied according to the complete randomized design (CRD) with four replicates. The experiments included two factors: the first factor plant species (three plant species),and the second factor concentration of plant extracts (2.5%, 5% and 10%) and their impact on germination rate and inhibition of seedling growth. The results of the first experiment recorded inhibition percentage reached (90%) at 5% and 10% from Nerium extracts compared with the same concentrations from Olive and Castor which, recorded inhibitory rate reached (30-40%) respectively. While the concentration of 2.5% was the lowest inhibition rates for germination ratio of all plant extracts under study. The second experiment showed effect of the Nerium extract at a concentration (10%) by recording completely inhibition ratio (100%), compared with the same concentrations of Castor and Olive extracts, which recorded the lowest mean growth inhibitory for all extracts. The results of the current study indicate the possibility of using natural products of plant origin as alternatives to chemical pesticides as one of the means of biological control in the management program agricultural sector with further studies of the future.
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Hani, Ghada Mazin, Sahib M. Mahdi, and Amer H. Majeed. "Using Dry Olive Leaves for Oil Pipes Corrosion Inhibitor." European Journal of Engineering Research and Science 4, no. 3 (March 13, 2019): 73–76. http://dx.doi.org/10.24018/ejers.2019.4.3.1060.
Повний текст джерелаАнотація:
At the present time the use of corrosion inhibitors is a good solution to maintain or reduce the corrosion problems in the equipment, so nowadays the production of inhibitors all non-toxic, natural and cheap is the most important demand. In this study dry olive leaves extraction used as inhibitor in a temperatures similar to the average temperature in Iraq, so as to bring the picture of reality as much as possible, at (25 °C and 45 °C) and solution was prepared in the following concentration to stimulate the field environment for oil extracting 19.2 % of sodium chloride (NaCl), 8% of calcium chloride (CaCl), 1.08 % of magnesium chloride (MgCl) and saturated with Co2 gas, the corrosion rate of steel sample was measured be using a potentiostat device with reference of calomel electrode. The result was, the dry olive leaves extract is an effective inhibitor material. Dry olive leaves extraction inhibitor has a low corrosion rate at 25OC and 45OC and the Corrosion rate decrease with increasing temperature for oil extraction solution, From the EDS test found that high percentage of carbon and oxygen caused to produce many compounds like the phenolic compound of the olive leaves is the responsible of inhabitation effect of the olive leaves.
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Hani, Ghada mazin, Sahib M. Mahdi, and Amer H. Majeed. "Using Dry Olive Leaves for Oil Pipes Corrosion Inhibitor." European Journal of Engineering and Technology Research 4, no. 3 (March 13, 2019): 73–76. http://dx.doi.org/10.24018/ejeng.2019.4.3.1060.
Повний текст джерелаАнотація:
At the present time the use of corrosion inhibitors is a good solution to maintain or reduce the corrosion problems in the equipment, so nowadays the production of inhibitors all non-toxic, natural and cheap is the most important demand. In this study dry olive leaves extraction used as inhibitor in a temperatures similar to the average temperature in Iraq, so as to bring the picture of reality as much as possible, at (25 °C and 45 °C) and solution was prepared in the following concentration to stimulate the field environment for oil extracting 19.2 % of sodium chloride (NaCl), 8% of calcium chloride (CaCl), 1.08 % of magnesium chloride (MgCl) and saturated with Co2 gas, the corrosion rate of steel sample was measured be using a potentiostat device with reference of calomel electrode. The result was, the dry olive leaves extract is an effective inhibitor material. Dry olive leaves extraction inhibitor has a low corrosion rate at 25OC and 45OC and the Corrosion rate decrease with increasing temperature for oil extraction solution, From the EDS test found that high percentage of carbon and oxygen caused to produce many compounds like the phenolic compound of the olive leaves is the responsible of inhabitation effect of the olive leaves.