Дисертації з теми "Nucleus Pulposus Regeneration"
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Kim, Anne Jungjoo. "In situ regeneration of the nucleus pulposus." Diss., Search in ProQuest Dissertations & Theses. UC Only, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3359553.
Повний текст джерелаSource: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3639. Adviser: Jeffrey C. Lotz.
Simson, Jacob A. "Physical analysis of collagen-GAG composite scaffolds for nucleus pulposus tissue regeneration." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/57874.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (p. 27-28).
In this study biomaterial scaffolds for regeneration of nucleus pulposus were developed by freeze drying slurries with different proportions of collagen II (CII), chondroitin-6-sulfate (CS), and hyaluronic acid (HA). The scaffolds were analyzed using biochemical assays to determine final composition. Chemically cross-linked scaffolds were analyzed to determine pore size and cross-link density. It was determined that every material type contained large enough pore size (275 gm) to seed nucleus pulposus cells and mesenchymal stem cells. The addition of CS to the scaffold increased pore size. It was also found that increasing levels of CS and HA resulted in lower cross-link density. These materials will be used next in In Vitro studies to determine their viability as regenerative tissue engineering constructs.
by Jacob A. Simson.
S.B.
Aladin, Kaderbatcha Darwesh Mohideen. "Nanoscale structure-property and macroscale biomechanical function of nucleus pulposus in health, disease and regeneration." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45197143.
Повний текст джерелаEhlicke, Franziska [Verfasser]. "Entwicklung eines injizierbaren Zell-Matrix-Komposites zur Regeneration der Bandscheibe (Nucleus pulposus) : Einfluss verschiedener Stimuli auf die Differenzierung von humanen mesenchymalen Stammzellen in Richtung Nucleus pulposus-Zellen / Franziska Ehlicke." Aachen : Shaker, 2014. http://d-nb.info/1049379608/34.
Повний текст джерелаStrassburg, Sandra. "An In Vitro Culture System to Study Human Mesenchymal Stem Cell / Nucleus Pulposus Cell Interactions : Implications for Intervertebral Disc Regeneration." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521587.
Повний текст джерелаRodrigues, Pinto Ricardo Pedro Ferreira. "Isolation and phenotypic characterisation of human notochordal cells : implications for the development of cell-based therapies for intervertebral disc degeneration." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/isolation-and-phenotypic-characterisation-of-human-notochordal-cells-implications-for-the-development-of-cellbased-therapies-for-intervertebral-disc-degeneration(8d5cbfdd-edd0-458c-a048-554f6a2c830b).html.
Повний текст джерелаKhan, Shahnaz. "The effect of the intervertebral disc microenvironment on disc cell and mesenchymal stem cell behaviour : implications for disc degeneration and regeneration." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/the-effect-of-the-intervertebral-disc-microenvironment-on-disc-cell-and-mesenchymal-stem-cell-behaviour-implications-for-disc-degeneration-and-regeneration(b5629a75-4cb0-45d8-affb-2b936d9408e1).html.
Повний текст джерелаLok, Peter Yin Cheung. "Development of a novel minimally invasive scaffold system for spinal disc repair." Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/12583.
Повний текст джерелаFrancisco, Aubrey Therese. "Laminin-Functionalized Polyethylene Glycol Hydrogels for Nucleus Pulposus Regeneration." Diss., 2013. http://hdl.handle.net/10161/8204.
Повний текст джерелаIntervertebral disc (IVD) disorders and age-related degeneration are believed to contribute to low back pain. There is significant interest in cell-based strategies for regenerating the nucleus pulposus (NP) region of the disc; however, few scaffolds have been evaluated for their ability to promote or maintain an immature NP cell phenotype. Additionally, while cell delivery to the pathological IVD has significant therapeutic potential for enhancing NP regeneration, the development of injectable biomaterials that retain delivered cells, promote cell survival, and maintain or promote an NP cell phenotype in vivo remains a significant challenge. Previous studies have demonstrated NP cell - laminin interactions in the NP region of the IVD that promote cell attachment and biosynthesis. These findings suggest that incorporating laminin ligands into biomaterial scaffolds for NP tissue engineering or cell delivery to the disc may be beneficial for promoting NP cell survival and phenotype. In this dissertation, laminin-111 (LM111) functionalized poly(ethylene glycol) (PEG) hydrogels were developed and evaluated as biomaterial scaffolds for cell-based NP regeneration.
Here, PEG-LM111 conjugates with functional acrylate groups for crosslinking were synthesized and characterized to allow for protein coupling to both photocrosslinkable and injectable PEG-based biomaterial scaffolds. PEG-LM111 conjugates synthesized using low ratios of PEG to LM111 were found support NP cell attachment and signaling in a manner similar to unmodified LM111. A single PEG-LM111 conjugate was conjugated to photocrosslinkable PEG-LM111 hydrogels, and studies were performed to evaluate the effects of hydrogel formulation on immature NP cell phenotype in vitro. When primary immature porcine NP cells were seeded onto PEG-LM111 hydrogels of varying stiffnesses, softer LM111 presenting hydrogels were found to promote cell clustering and increased levels of sGAG production as compared to stiffer LM111 presenting and PEG-only gels. When cells were encapsulated in 3D gels, hydrogel formulation was found to influence NP cell metabolism and expression of proposed NP phenotypic markers, with higher expression of N-cadherin and cytokeratin 8 observed for cells cultured in softer (<1 kPa) PEG-LM111 hydrogels.
A novel, injectable PEG-LM111 hydrogel was developed as a biomaterial carrier for cell delivery to the IVD. PEG-LM111 conjugates were crosslinked via a Michael-type addition reaction upon the addition of PEG-octoacrylate and PEG-dithiol. Injectable PEG-LM111 hydrogel gelation time, mechanical properties, and ability to retain delivered cells in the IVD space were evaluated. Gelation occurred in approximately 20 minutes without an initiator, with dynamic shear moduli in the range of 0.9 - 1.4 kPa. Primary NP cell retention in cultured IVD explants was significantly higher over 14 days when cells were delivered within a PEG-LM111 hydrogel carrier, as compared to cells in liquid suspension.
The studies presented in this dissertation demonstrate that soft, LM111 functionalized hydrogels may promote or maintain the expression of specific markers and cell-cell interactions characteristic of an immature NP cell phenotype. Furthermore, these findings suggest that this novel, injectable laminin-functionalized biomaterial may be an easy to use and biocompatible carrier for delivering cells to the IVD.
Dissertation
Tai, Pei-Wen, and 戴珮雯. "Developing hyaluronic acid-silk fibroin double-network hydrogel for nucleus pulposus regeneration." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/k52xdj.
Повний текст джерелаCheng, Yung-Hsin, and 鄭詠馨. "Thermosensitive Chitosan-Gelatin-Glycerol Phosphate Hydrogel as Cell Carrier for Nucleus Pulposus Regeneration." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/73418695360656835403.
Повний текст джерела國立臺灣大學
醫學工程學研究所
96
Disc degeneration is strongly associated with back pain and herniation resulting in the cost of health care decreasing. Some evidence shows that disc degeneration originates in the nucleus pulposus (NP). However, current treatments such as physical therapy, pharmaceutical treatment, spinal fusion, artificial disc replacement and discectomy attempt to reduce pain rather than repair the degenerated disc. Cell–based therapies are aimed at repairing the degenerated disc, which is a potential treatment of disc degeneration. Therefore, the purpose of this study was to prepare the thermosensitive hydrogel as cell carrier of NP cells. In this study, the thermosensitive chitosan/gelatin/β- glycerol phosphate disodium salt hydrogels (C/G/GP hydrogels) was investigated. Nucleus pulposus cells which were harvested from the intervertebral discs of the adult New Zealand white rabbits were encapsulated in C/G/GP hydrogels. The gelation temperature, gelation time and gel strength were evaluated by rheometer. Compared with the formulations using only C/GP, the gel strength of C/G/GP was increased at 37°C. In the C/G/GP system, raising the concentration of gelatin resulted in a decrease in the gelation temperature and gelation time. The results of the in vitro cytotoxicity showed that the C/GP and the C/G/GP hydrogel are biocompatible. In the degradation test, raising the concentration of gelatin seems to increase the percentage of weight loss. The ratio of sulfated glycosaminoglycan (GAG) to DNA of NP cells cultured in hydrogels showed significantly higher than monolayer-cultured at the end of 3-week. Compared with monolayer-cultured, the mRNA expression of type II Collagen, Aggrecan, MMP-3, MMP-9, IGF-1, BMP-7 and TGF-β in hydrogel-cultured NP cells was significantly enhanced. The results showed that the C/G/GP solution remains liquid at room temperature but form the hydrogel at approximate body temperature under a neutral pH. The formed hydrogel is biocompatible and biodegradable. As three-dimensional carrier for NP cell culture, these results suggest the C/G/GP hydrogel is a suitable scaffold for the cell culture. These features make the C/G/GP hydrogel potential application as an injectable cell carrier for NP regeneration.
Shu-Hua, Yang. "Regeneration of Nucleus Pulposus by Using Gelatin/Chondroitin-6-Sulfate/Hyaluronan Tri-copolymer Scaffold." 2005. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-0407200510540500.
Повний текст джерелаYang, Shu-Hua, and 楊曙華. "Regeneration of Nucleus Pulposus by Using Gelatin/Chondroitin-6-Sulfate/Hyaluronan Tri-copolymer Scaffold." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/21537453878599164800.
Повний текст джерела國立臺灣大學
醫學工程學研究所
93
【Study Design】A bioactive material was developed with the composition of gelatin, chondoitin-6-sulfate, and hyaluronan. Human nucleus pulposus cells were 3- dimensionally cultured in this tri-copolymer scaffold in vitro, and then their cell proliferation and matrix productivity were measured by ELISA assays, immuno- histochemical staining, and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). 【Objective】To evaluate the feasibility of gelatin/chondoitin-6-sulfate/hyaluronan tri-copolymer serving as a bioactive scaffold for regeneration of human nucleus pulposus. 【Summary of Background Data】Degeneration of the intervertebral disc starts from the nucleus pulposus and finally leads to various associated spinal disorders. Symptomatic intervertebral disc degeneration results in substantial pain and increased health cost. Current treatment options for degenerative disc disease may alleviate clinical symptoms but are not able to stop and sometimes even would accelerate the degeneration in the intervertebral disc. Tissue engineering approaches for treating degenerative intervertebral discs aim to promote tissue regeneration then retard or even reverse degenerative process. Combination of cells and bioactive scaffold is one of the promising tissue engineering methods for regeneration of the intervertebral disc including the nucleus pulposus. 【Materials and Methods】The gelatin/chondroitin-6-sulfate/hyaluronan tri-copolymer was fabricated into scaffold discs and was cross-linked by glutaraldehyde. Nucleus pulposus cells were isolated from human nucleus pulposus tissues and expanded in monolayer culture. Each scaffold disc was seeded with 1x106 cells and then cultured in vitro for 4 weeks. The cell-scaffold hybrids as well as the control scaffolds were then analyzed on their cell viability/proliferation, content of sulfated glycos- aminoglycans, mRNA expression of selected genes, histological and immuno- histochemical studies. 【Results】The cell-scaffold hybrids demonstrated active cell viability/proliferation and their net increases of sulfated glycosaminoglycans were progressively higher during a 4-week cultivation. In comparison to monolayer cells, scaffold-cultured cells showed significantly higher mRNA expression in type II collagen, aggrecan, Sox9, TGF-β1, and TIMP-1. Expressions of mRNA were significantly suppressed in type I collagen, type X collagen, IL-1 and Fas-associating death domain protein. Histological studies showed newly synthesized matrices containing glycos- aminoglycans deposits and type II collagen in the cell-scaffold hybrids while the scaffold substrate lost its originally entrapped glycosaminoglycans during the in vitro cultivation. 【Conclusions】The gelatin/chondroitin-6-sulfate/hyaluronan tri-copolymer scaffold has positive effects on cell proliferation and matrix production to human nucleus pulposus cells. The tri-copolymer scaffold is promising bioactive material that warrants further investigation into their application in regeneration of nucleus pulposus. 【Future Works】After a successful in vitro experiment on culturing human nucleus pulposus cells in the tri-copolymer scaffold, an animal study is a mandatory step before proceeding to clinical application. In addition, searching for healthy and viable nucleus pulposus cells is also important for promoting cell proliferation and matrix productivity. Combination of mesenchymal stem cells and current/future bioactive scaffolds is also worthwhile being investigated.
Cheng, Yung-Hsin, and 鄭詠馨. "Thermosensitive Chitosan/Gelatin/Glycerol Phosphate Hydrogel as a Sustained Release System of Ferulic Acid for Nucleus Pulposus Regeneration." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/51055450736492489282.
Повний текст джерела國立臺灣大學
醫學工程學研究所
100
Disc degeneration is strongly associated with back pain and herniation that increase the costs of health care. The degeneration of intervertebral disc (IVD) could be divided into 5 stages. In the first and second stages, there are no significant symptoms but could be traced by magnetic resonance imaging or computed tomography-scan. Generally, no aggressive treatment would be processed in the clinics. Recent studies indicated that overproduction of reactive oxygen species (ROS) may accelerate the degenerative process of IVD and associate with apoptosis of nucleus pulposus (NP) cells and degradation of extracellular matrix. Ferulic acid (FA) is an excellent antioxidant and relatively stable in air. FA has been proven to have ability to prevent ROS-induced diseases. The object of the study was aimed to evaluate the possible therapeutic effect of FA on hydrogen peroxide (H2O2)-induced oxidative stress NP cells and the feasibility of use the thermosensitive chitosan/gelatin/glycerophosphate (C/G/GP) hydrogel as a sustained release system of FA for early treatment in IVD degeneration. In the study, NP cells were harvested from the IVD of New Zealand rabbits. The results showed that 500 μM of FA might be the threshold to treat NP cells without cytotoxicity. Post-treatment of FA on H2O2-induced oxidative stress NP cells significantly up regulated the expression of aggrecan, type II collagen and BMP-7 and down regulated the expression of MMP-3 in mRNA level. Post-treatment of FA on H2O2-induced oxidative stress NP cells could restore the production of sulfated glycosaminoglycans (GAGs) and inhibit the apoptosis caused by H2O2. The results showed that the release of FA from C/G/GP hydrogel could decrease the H2O2-induced oxidative stress. Post-treatment of FA-incorporated C/G/GP hydrogel on H2O2-induced oxidative stress NP cells showed up-regulation of aggrecan and type II collagen and down-regulation of MMP-3 in mRNA level. The results of sulfated GAGs to DNA ratio and alcian blue staining revealed that the GAGs production of H2O2-induced oxidative stress NP cells could reach to normal level. The results of caspase-3 activity and TUNEL staining indicated that FA-incorporated C/G/GP hydrogel decreased the apoptosis of H2O2-induced oxidative stress NP cells. The results showed that FA was successfully immobilized on C/G/GP hydrogel by N-(3-dimethylaminopropyl)-N''-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) crosslinking method. The gelation temperature of the FA-immobilized C/G/GP hydrogel was 31.80 degree celsius under neutral pH. Post-treatment of FA-immobilized C/G/GP hydrogel on H2O2-induced oxidative stress NP cells showed down-regulation of MMP-3 and up-regulation aggrecan and type II collagen in mRNA level. The sulfated GAGs production of H2O2-induced oxidative stress NP cells could be increased to the normal level in the post-treatment of FA-immobilized C/G/GP hydrogel group. The results of caspase-3 activity and TUNEL staining showed that the apoptosis of H2O2-induced oxidative stress NP cells could be inhibited by post-treatment of FA-immobilized C/G/GP hydrogel. From the results of the study, FA could be used as a therapeutic molecule for NP regeneration and FA-incorporated C/G/GP hydrogel might be potentially applied as a long-term release system. The immobilization of FA on C/G/GP hydrogel could significantly prolong the release period of FA. These results suggest that combination of FA and thermosensitive C/G/GP hydrogel can treat NP cells from the damage caused by oxidative stress and may apply in minimally invasive surgery for NP regeneration in the future.
Windisch, Leah Marianne. "Design and Evaluation of a Disulphide-crosslinked Hyaluronan Hydrogel for Regeneration of the Intervertebral Disc." Thesis, 2008. http://hdl.handle.net/1807/17236.
Повний текст джерела