Дисертації з теми "Nucleic acid based drug"
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Wong, Frances M. P. "Lipid-based vehicles for nucleic acid drugs." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0015/NQ56644.pdf.
Повний текст джерелаSatyal, Uttam. "Efficient Drug and Nucleic Acid Delivery Systems based on Synthetic Amphiphiles with Tuned Oil/Water Interfaces." Diss., Temple University Libraries, 2018. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/531985.
Повний текст джерелаPh.D.
Today, drugs are an integral part of healthy human life, with new drug entities being introduced every year in clinic. The advancement of drug development brings complexity and variation, in terms of both physical and chemical properties. Some of these physicochemical characteristics are many times suboptimal, eventually requiring robust delivery systems that can precisely deliver the drugs to the desired tissues. Although many materials have been studied for the generation of drug delivery systems, there is always a need for biomaterials with better properties that can translate into superior delivery systems. In this context, new drug delivery systems that are interface-engineered at materials level for better stability and delivery efficiency in vitro and in vivo are introduced in this dissertation. In the first part of the dissertation, novel oil/water interface-engineered amphiphilic block copolymer micelles that were previously introduced by our lab were assessed for their stability in the presence of various esterase enzymes present in serum and on blood vessel walls, normally encountered by drug delivery systems on route to the targeted tissues. I also assessed the vulnerability of the polymeric micelles in presence of enzymes typically present either inside the tumor cells or secreted in the tumor microenvironment. I revealed the selective stability of empty- and docetaxel-loaded polymeric micelles to enzymatic degradation en route/in tumors and I have correlated this selective stability with polymer structure and interfacial engineering mentioned above. The unique delivery capabilities of interfacial-engineered polymeric micelles were tested in vivo using a mouse model of triple negative breast cancer. We proved that our novel engineered triblock copolymer-based drug delivery systems are superior to similar delivery systems made out of standard diblock copolymer micelles and also to the clinically used Taxotere® formulation towards cancer cell killing and tumor treatment, without displaying any significant toxicity in experimental animals. The second part of the dissertation focuses on the development and assessment of a pyridinium-based pseudo-gemini surfactant that combined the high nucleic acid packaging capacity of pyridinium lipids with the high transfection efficiency of gemini surfactants while displaying a reduced associated cytotoxic effect. I have analyzed the temperature treatment on compaction of nucleic acids into lipoplexes and I have established a high temperature annealing method for this purpose. This novel formulation technique allowed a substantial reduction of the amount of amphiphiles required to compact a specific amount of nucleic acids. This in turn also reduced the cytotoxic effect associated with the use of pyridinium amphiphiles. The effect of inclusion of colipids to lipoplex compaction, the robustness and the transfection efficiency of the lipid/nucleic acid lipoplex systems were assessed in detail, and correlations between formulation composition and biological activity were established. I was also able to show for the first time that pyridinium pseudo-gemini surfactants were able to compact different types of nucleic acids, including pDNA, mRNA and siRNA at lower charge ratios than standard, state-of-the art formulations used for this purposes. I also showed that irrespective to the nucleic acid compacted within the lipoplexes, the novel amphiphiles can efficiently deliver the cargo into the targeted cells even in the presence of very high concentration of serum, a premise for future use of these amphiphiles and formulations in vivo.
Temple University--Theses
Pel, Joel. "A novel electrophoretic mechanism and separation parameter for selective nucleic acid concentration based on synchronous coefficient of drag alteration (SCODA)." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/13402.
Повний текст джерелаFolly, da Silva Constantino Laura. "An effective layered workflow of virtual screening for identification of active ligands of challenging protein targets." Thesis, University of Iowa, 2017. https://ir.uiowa.edu/etd/5754.
Повний текст джерелаBELGIOVINE, GIULIANA. "Delivery of small interfering RNA into airway epithelial cells. Downregulation of the pro-inflammatory cytokine high mobility group BOX 1." Doctoral thesis, Università di Foggia, 2016. http://hdl.handle.net/11369/338921.
Повний текст джерелаAkhras, Michael S. "Nucleic Acid Based Pathogen Diagnostics." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4684.
Повний текст джерелаPatogena organismer smittas till värd organismen genom alla möjliga kontaktnätverk och skapar en mångfald olika sjukdomstillstånd. Dock är det fortfarande vanligt förekommande behandlingsbara infektiösa sjukdomar som orsakar den största hälsoförlusten, sett från ett globalt perspektiv. Bill och Melinda Gates Stiftelsen samarbetade med RAND kooperation för att forma “The Global Health Diagnostics Forum”. Deras mål var att etablera och analysera matematiska modeller för vilka effekter en ny diagnostisk metod utrustat för fältarbete skulle ha i utvecklingsländer. Resultaten var häpnadsveckande, med potentiellt miljoner av liv som skulle kunna räddas på en årlig basis. Den etablerade standarden för diagnostik av patogena bakterier har länge varit kultiveringsmedia baserad. Miljö specialiserade biologer har estimerat att mindre än 1 % av alla bakterie arter går att kultivera. Dock erbjuder genetiska analyser potentialen att kunna identifiera alla mikrober från alla de biologiska rikena. Nukleinsyrebaserade diagnostiska metoder har märkbart förbättrats över de senaste årtionden. Nya tekniker erbjuder utökad sensitivitet, selektivitet, sänkta kostnader och parallella analyser av patient prover. Dock är de flesta metoderna begränsade till standardiserade laboratoriemiljöer. För att konstruera en väl fungerande diagnostisk fältutrustning för användning i problem områden, behöver världsledande tekniker identifieras och kombineras. Fokuseringsområdet för denna doktorsavhandling har varit att utveckla och utföra nukleinsyrebaserade metoder för patogen diagnostik. Metoder och experimentella utförande applicerades på två distinkta system i) sökning av antibiotika resistens relaterade mutationer i den patogena bakterien Neisseria gonorrhoeae och ii) genotypning av det cancer orsakande Humana Papillomaviruset (HPV). Den första delen av studien inriktade sig mot utveckling av snabba, direkta och multiplexa Pyrosekvenserings baserade nukleinsyreanalyser. Med förbättrad provprepareringsmetodologi kunde vi detektera multipla HPV infektioner med högre sensitivitet än vad tidigare beskrivits med liknande metodologi. Den andra delen av studien fokuserades på multiplexa nukleinsyre amplifikationer med “Molecular Inversion Probe” tekniken med sista steg Pyrosekvenserings analys. “PathogenMip assay” erbjuder ett komplett detektionsprotokoll för alla kända patogena organismer. Vi introducerar även den nya “Connector Inversion Probe”, en “Padlock Probe” kapabel att genomföra kompletta gap fyllningar för multiplex nukleinsyre amplifiering.
QC 20100624
Elmén, Joacim. "Nucleic acid based therapeutic approaches /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-047-8/.
Повний текст джерелаAllsop, Julie Kay. "Development of nucleic acid vaccines for mucosal delivery." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263104.
Повний текст джерелаZhou, Chenguang. "NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204.
Повний текст джерелаO'Daniel, Peter Ivo. "Exploring structural diversity in nucleoside and nucleic acid drug design." Diss., Available online, Georgia Institute of Technology, 2005, 2005. http://etd.gatech.edu/theses/available/etd-08252005-130946/.
Повний текст джерелаBarefield, E. Kent, Committee Member ; Beckham, Haskell W., Committee Member ; Doyle, Donald F., Committee Member ; Weck, Marcus, Committee Member ; Seley, Katherine L., Committee Chair.
Kedge, Jonathan L. "Synthesis of ferrocene nucleic acid monomers and ferrocene containing drug candidates." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7424/.
Повний текст джерелаGarner, Mark. "Kinetic and mechanistic studies of Cisplatin derivatives with nucleic acid fragments." Thesis, University of York, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306475.
Повний текст джерелаSchaffert, David Henning. "Precise oligoethylenimine-based carriers for nucleic acid delivery." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-151686.
Повний текст джерелаO'Meara, Deirdre. "Molecular Tools for Nucleic Acid Analysis." Doctoral thesis, Stockholm : Tekniska högsk, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3220.
Повний текст джерелаPerin, Danilo. "Biomaterials for biotechnological applications: synthesis and activity evaluations." Doctoral thesis, Università degli studi di Trieste, 2010. http://hdl.handle.net/10077/3518.
Повний текст джерелаA biomaterial was defined as any non-living material used in a medical device that interacts with biological systems. Many different applications involve the use of biomaterials: pharmacology, controlled drug release, extracorporeal devices (contact lens, emodialysis devices, cardiopulmonary bypass oxygenators), artificial prostheses. One of the most interesting biomaterials application regards the release of nucleic acids and their derivatives as therapeutic agents. These molecules, defined as “nucleic acid base drugs” (NABDs), allows highly targeted cellular metabolism modifications. The aim of this research project concerns the characterization of biomaterials for biotechnological applications and evaluation of their activities. In particular, because of the great therapeutics and commercial interest and the delivery problems that are largely unresolved, the attention is focused on the study of new delivery systems for siRNA, proposed as model system as they represent the most common and best characterized NABD. SiRNA proved to be useful for what concerns the in-stent restenosis, pathology implying the re-occlusion of the artery due to the iper-proliferation of smooth muscle cells induced by the presence of the stent, a metal prosthesis that is applied to avoid the elastic recoil of the artery wall after balloon angioplasty. In this system, the siRNA should act as an anti-proliferative of smooth muscle cells without interfering with endothelial cells. In order to design an appropriate delivery system, it is crucial a precise structural and dimensional characterization of polymeric mesh. This purpose was achieved by the use of various techniques such as Rheology, low field NMR and Cryoporometry. Rheology allows the evaluation of the macroscopic mechanical properties of the system under investigation (Young's and shear modulus for example). Low field NMR, instead, allows evaluating the microscopic properties and, coupled to the rheology, provides an estimation of the polymeric mesh size distribution. Cryoporometry is another method to assess the mesh size distribution. In vivo release tests represent the final step of the experimental process. The polymeric system ability to carry and deliver the liposome-siRNA complexes, was tested in culture models of smooth muscle cells and endothelial cells. The attention has been focused on polymeric hydrogels, whose biocompatibility and biodegradability is well known: • Alginate (polymeric concentration 1% - 2% - 3%), crosslinked by Ca2+ or Cu2+ water solution • Pluronic™ F127 18% in water • Dextran 5% or 30% methacrylate (respectively D40MA5% and D500MA30%; polymeric concentration 5%) crosslinked by UV • Gel systems derived from benzofulvene And polymeric blends: • Pluronic™-alginate hydrogels respectively at 18% and 2% in water • Dextran methacrylate-alginate respectively at 5% and 3% in water (A3D40MA5% or A3D500MA30%)
Un biomateriale è definito come qualsiasi materiale non-vivente utilizzato in un dispositivo medico interagente con i sistemi biologici. Diverse applicazioni comportano l'uso di biomateriali: farmacologia, sistemi di controllato rilascio di farmaci, dispositivi extracorporei (lenti a contatto, dispositivi per emodialisi, ossigenatori, bypass cardiopolmonari, ecc.), protesi artificiali. Una delle applicazioni più interessanti dei biomateriali riguarda il rilascio di acidi nucleici e loro derivati come agenti terapeutici. Queste molecole, definite come " nucleic acid base drugs " (NABDs), consentono modifiche altamente mirate del metabolismo cellulare. L'obiettivo di questo progetto di ricerca riguarda la caratterizzazione dei biomateriali per applicazioni biotecnologiche e la valutazione delle loro attività. In particolare, dato il notevole interesse terapeutico e commerciale, oltre ai problemi di delivery tutt’ora in gran parte irrisolti, l'attenzione è stata focalizzata sullo studio di nuovi sistemi di somministrazione di siRNA, proposti come sistema modello in quanto rappresentano il più comune e meglio caratterizzato NABD. I siRNA si sono rivelati utili per il trattamento della ristenosi in-stent, una patologia che comporta la ri-occlusione dell'arteria in seguito alla iper-proliferazione delle cellule muscolari lisce indotta dalla presenza dello stent, una protesi di metallo applicata per evitare la contrazione elastica della parete arteriosa in seguito ad angioplastica con palloncino. In questo sistema, il siRNA dovrebbe agire come un anti-proliferativo delle cellule muscolari lisce, senza interferire con le cellule endoteliali. Al fine di progettare un adeguato sistema di rilascio, è di fondamentale importanza una precisa caratterizzazione strutturale e dimensionale delle maglie polimeriche. Questo scopo è stato raggiunto mediante l’utilizzo di varie discipline quali la Reologia, l’NMR a basso campo e la Crioporimetria. La Reologia permette una valutazione macroscopica delle proprietà meccaniche del sistema in esame (ad esempio attraverso il modulo di Young ed il modulo di taglio). L’NMR a basso campo, invece, permette di valutare le proprietà microscopiche e, accoppiato alla reologia, fornisce una stima della distribuzione dimensionale delle maglie polimeriche. La Crioporimetria è metodo alternativo per la valutazione della distribuzione dimensionale delle maglie. I test di rilascio in vivo rappresentano l'ultima fase del processo sperimentale. La capacità del sistema polimerico di trasportare e rilasciare liposomi complessati a siRNA, è stata valutata in modelli di cellule muscolari lisce e cellule endoteliali in coltura. L’attenzione e stato focalizzata soprattutto su sistemi idrogel polimerici la cui biocompatibilità e biodegradabilità è ben nota: • Alginato (concentrazione polimero 1% - 2% - 3%), reticolato attraverso soluzioni acquose di Ca2+ o Cu2+ • Pluronico F127 al 18% in acqua • Destrano 5% o 30% metacrilato (rispettivamente D40MA5% e D500MA30% ad una concentrazione polimerica pari al 5% in acqua), reticolati tramite UV • Sistemi gel derivati da benzofulvene E le miscele polimeriche costituite da: • Idrogel di pluronico-alginato rispettivamente al 18% e 2% in acqua • Destrano metacrilato-alginato rispettivamente al 5% e 3% in acqua (A3D40MA5% o A3D500MA30%)
XXII Ciclo
1980
Goujon, Jennyfer. "Towards the development of a novel colourimetric nucleic acid biosensor based on peptide nucleic acid-functionalised polydiacetylene liposomes." Thesis, Heriot-Watt University, 2009. http://hdl.handle.net/10399/2306.
Повний текст джерелаAndersson, Sara. "Development of Peptide Nucleic Acid Based Artificial Ribonucleases, PNAzymes." Thesis, Mälardalens högskola, Akademin för hållbar samhälls- och teknikutveckling, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-12280.
Повний текст джерелаXu, Gaolian. "Developing new synthetic tools for nucleic acid based diagnostics." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7704/.
Повний текст джерелаDaher, Rana. "Recombinase polymerase amplification technology : Assessment for nucleic acid-based acid-based point-of-care diagnostics." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26269.
Повний текст джерелаThis dissertation consists of an exhaustive study on an emerging technology for isothermal amplification of nucleic acids called recombinase polymerase amplification (RPA). The introduction of this thesis is a detailed description of the RPA. This review documents and discusses the various applications of this technology by pointing to the current knowledge about RPA for diagnostic applications. Despite the complex composition of RPA (6 to 7 proteins in the same reaction mixture), the latter was shown to be rapid (generating results in < 20 min), specific and sensitive (detecting few target genome copies), and applied widely in different fields. Based on these advantages, we assume that RPA has a flexibility allowing it to be used for the rapid diagnosis of infectious diseases thus reducing time-to-result to less than an hour. Consequently, it will be possible to integrate RPA in microfluidic platforms providing a lab-on chip system. The first part of this doctoral project generated additional guidelines for RPA primers/probes design to develop specific RPA diagnostic assays. Second, we developed an RPA diagnostic test for the detection of group B streptococci, responsible for sepsis and meningitis in newborns. This assay was the first to evaluate RPA with human clinical samples. This diagnostic test was compared to a reference method, the polymerase chain reaction (PCR). This demonstration with clinical samples served to carry out the final objective of this project that was to automate RPA in a miniaturized microfluidic centripetal system. Collaboration with engineers and experts in materials has generated the microfluidic device called "blade" and the instrument involved in the operation of various mechanistic tasks. These preliminary results suggested that it will be important to provide an automated system applicable at bedside. Consequently, it will be possible to perform a complete analysis of infectious agents in less than an hour without the need for complex procedures for the preparation and transport of clinical specimens or the assistance of qualified personnel.
Johns, Rachel Elizabeth. "Delivery of anti-inflammatory nucleic acid therapeutics using smart polymeric carriers /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/8080.
Повний текст джерелаHenriksson, Sara. "Application of Padlock Probe Based Nucleic Acid Analysis In Situ." Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-128446.
Повний текст джерелаBamberger, Denise Nadine [Verfasser]. "Polysaccharide-based nanoparticles for nucleic acid delivery / Denise Nadine Bamberger." Mainz : Universitätsbibliothek Mainz, 2018. http://d-nb.info/1160897409/34.
Повний текст джерелаSchulz, Martin [Verfasser], and Felix von [Akademischer Betreuer] Stetten. "Microfluidic system integration for droplet based digital nucleic acid testing." Freiburg : Universität, 2020. http://d-nb.info/1229349278/34.
Повний текст джерелаIemsam-Arng, J. "Poly(ethylene) glycol based delivery systems for nucleic acid therapies." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1381001/.
Повний текст джерелаBacigalupo, Maria Candelaria Rogart. "Locked nucleic acid analogues for novel exciplex-based molecular probes." Thesis, University of Manchester, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538490.
Повний текст джерелаTakalkar, Sunitha. "Micro-/Nano- Materials Based Ultrasensitive Lateral Flow Nucleic Acid Biosensors." Diss., North Dakota State University, 2017. https://hdl.handle.net/10365/28376.
Повний текст джерелаNational Institutes of Health (NIH)
DOE-ND EPSCOR
NIH-COBRE (1P20GM09024-01A1)
Sizovs, Antons. "Development of Carbohydrate-based Diblock Polymers for Nucleic Acid Delivery." Diss., Virginia Tech, 2012. http://hdl.handle.net/10919/77089.
Повний текст джерелаPh. D.
Zhou, Zhun. "Design of polymer motifs for nucleic acid recognition and assembly stabilization." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437558800.
Повний текст джерелаLiang, Wanling, and 梁婉玲. "Formulation of nucleic acid with pH-responsive amphipathic peptides for pulmonary delivery." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/207996.
Повний текст джерелаFalconer, Robert Andrew. "Lipoamino acid based glycolipids for drug delivery." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392430.
Повний текст джерелаSabatino, David. "Expanding the size and shape of nucleic acids : studies on branched and heptose based nucleic acids." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103291.
Повний текст джерелаThe 2'-O-Lv and 2'-O-MMT ribonucleoside monomers served as building blocks for the assembly of a series of branched nucleic acid species (bRNA, bDNA, msDNA and hyperbranched or "dendritic" DNA/RNA) with discrete length, base composition and structure. These structures were synthesized via an iterative divergent-growth strategy, which facilitates the regioselective branching, deblocking and chain lengthening steps from a branchpoint core. These structures served as useful materials (bio-probes) as demonstrated by the biological studies performed with E. coli RNaseH and the yeast lariat RNA debranching enzyme (yDBr1). These studies not only led to the identification of novel branched nucleic acid inhibitors of yDBR1 and RNase H, but also provided new insights about the substrate specificity of these important enzymes.
This thesis also describes the synthesis of a new nucleic acid form, the so-called "oxepane nucleic acids" (ONAs), in which the pentofuranose ring of DNA and RNA was replaced with a 7-membered heptose sugar ring. ONA were found to be much more resistant towards nuclease degradation than natural DNA, an important feature if these analogues are to be used in biological media. Furthermore, ONAs exhibited cross-pairing with complementary RNA and were found to elicit E. coli RNaseH mediated degradation of the RNA strand. These finding are significant because oligonucleotide-directed RNase H degradation of the target RNA is a key determinant for the gene-specific inhibitory potency of antisense oligonucleotides. When comparing the rates of RNase H-mediated degradation induced by 5 (furanose), 6 (2'-ene-pyranose) and 7 (oxepane) membered ring oligonucleotides, the following trend was observed: DNA > 2'-ene-pyranose NA > ONA. The implications of these results are discussed in the context of our current understanding of the catalytic mechanism of the enzyme, particularly with regard to the required flexibility of the oligonucleotide strands that bind to the RNA target. Hence, ONAs are useful tools for biological studies and provide new insights into the structure/function of natural and alternative genetic systems.
Roark, Brandon Kyle. "Nucleic Acid-Driven Quantum Dot-Based Lattice Formations for Biomedical Applications." Thesis, The University of North Carolina at Charlotte, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10619578.
Повний текст джерелаWe present a versatile biosensing strategy that uses nucleic acids programmed to undergo an isothermal toehold mediated strand displacement in the presence of analyte. This rearrangement results in a double biotinylated duplex formation that induces the rapid aggregation of streptavidin decorated quantum dots (QDs). As biosensor reporters, QDs are advantageous to organic fluorophores and fluorescent proteins due to their enhanced spectral and fluorescence properties. Moreover, the nanoscale regime aids in an enhanced surface area that increase the number of binding of macromolecules, thus making cross-linking possible. The biosensing transduction response, in the current approach, is dictated by the analysis of the natural single particle phenomenon known as fluorescence intermittency, or blinking is the stochastic switching of fluorescence intensity ON (bright) and OFF (dark) states observed in single QD or other fluorophores. In contrast to binary blinking that is typical for single QDs, aggregated QDs exhibit quasi-continuous emission. This change is used as an output for the novel biosensing techniques developed by us. Analysis of blinking traces that can be measured by laser scanning confocal microscopy revealed improved detection of analytes in the picomolar ranges. Additionally, this unique biosensing approach does not require the analyte to cause any fluorescence intensity or color changes. Lastly, this biosensing method can be coupled with therapeutics, such as RNA interference inducers, that can be conditionally released and thus used as a theranostic probes.
Planonth, Songsak. "Peptide nucleic acid-encoded libraries for microarray-based high-throughput screening." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/5862.
Повний текст джерелаSlaitas, Andis. "Development of a new PNA analogue as a potential antisense drug and tool for life-science studies /." Stockholm : Karolinska institutet, 2004. http://diss.kib.ki.se/2004/91-7349-642-1/.
Повний текст джерелаKhater, Mohga Wagdy Yehia Mohamed. "Nanoparticle-based sensors for pathogen nucleic acid detection with interest for agriculture." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/667373.
Повний текст джерелаThis thesis aims at developing sensitive, affordable and portable biosensors based on nanomaterials for the determination of nucleic acid related to plant pathogens. The work strives to contribute to the keeping up in the advancements of biosensing systems relevant to plant infection diagnostics which would be an essential solution in the future to the issues of plant disease monitoring and food security. Following Chapter I, state-of-the-art on the latest trends in the development of advantageous biosensors based on both antibody and DNA receptors for early plant disease detection, as well as the use of different nanomaterials such as nanochannels and metallic nanoparticles for the development of innovative and sensitive biosensing systems for the detection of pathogens (i.e. bacteria and viruses) at the point-of-care is given. The next sections of this dissertation will describe three diagnostic biosensing strategies for the detection of citrus tristeza virus (CTV) related nucleic acid using electrical and optical transducing techniques. The electrical sensing of CTV through DNA hybridization based approach and the in situ amplified nucleic acid method will be achieved on carbon sensing substrate modified with gold nanoparticles, while paper-based sensors will be operated in lateral flow format for the gold nanoparticle-based optical detection of CTV. Furthermore, all aspects of the developed biosensing systems, from the bioassay and biosensor design to their development and optimization are presented in which will be organized in the following manner: Chapter III will present highly specific DNA hybridization sensor based on AuNP-modified SPCE employing label-free impedance for the detection of the CTV-related nucleic acid, together with dedicating emphasis to the study of electrodeposition time of AuNPs, whose precise particle size and shape will be required for the enhancement of DNA hybridization rate. A set of voltammetric studies of deposited AuNPs will be discussed. Particular attention will be paid for assembling the thiolated DNA probe as sensing layer for biosensor construction. The main sensor design aspects such as AuNPs size, probe DNA concentration and immobilization time together with DNA hybridization time will be optimized, in order to precisely select the best working conditions for this diagnostic platform. Chapter IV will cover the whole process undertaken for preparation of in situ nucleic acid amplification on gold nanoparticle-modified sensor for sensitive and quantitative detection of CTV. Plant disease (Citrus tristeza virus (CTV)) diagnostics was selected as relevant target for the demonstration of the proof-of-concept. This chapter will include two parts, the first one focuses on the design of RPA amplification assay, primers design, optimization of all essential bioassay aspects such as amplification temperature, volume and screening primers and finally the electrophoresis analysis for RPA products. The second part of this chapter will demonstrate label-free highly integrated in situ RPA amplification/detection approach at room temperature that takes advantage of the high sensitivity offered by gold nanoparticle-modified sensing substrates and electrochemical impedance spectroscopic (EIS) detection. Chapter V focuses on the application of isothermal nucleic acid amplification technology in simple lateral flow platform. The preparation of AuNP-based LFA for the highly sensitive direct detection of RPA amplified nucleic acid, the assembling of lateral flow step, the conjugation of AuNPs to the antibodies used for colorimetric detection, as well as the optimization of all working conditions and finally the analytical performance of the bioassay in LF will be explored. Moreover, aiming at truly achieving the point of care requirements of simple and affordable diagnostic technologies, the work here will present the possibility of amplifying nucleic acid without heat source and visual color detection. This approach would be of great potential as point of care diagnostics.
Pouchain, Delphine. "Peptide nucleic acid-encoded libraries for microarray-based enzymatic high-throughput screening." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/3227.
Повний текст джерелаTakalkar, Sunitha. "Ultrasensitive Lateral Flow Nucleic Acid Biosensors Based on Novel Macro-/Nano-Materials." Diss., North Dakota State University, 2017. https://hdl.handle.net/10365/26493.
Повний текст джерелаLin, Lina. "Synthesis, Structure, Function and Biomedical Studies of Nucleic Acid Derivatized with Selenium." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/biology_diss/77.
Повний текст джерела高橋, 洋介. "多足型DNAナノ構造体を利用した核酸医薬の標的指向化および体内動態制御に関する研究". Kyoto University, 2018. http://hdl.handle.net/2433/232325.
Повний текст джерелаNip, Lisa. "A nucleic acid-based bacterial message export system for cell-to-cell communication." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/110882.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (pages 36-38).
Communication within natural systems of eukaryotes and prokaryotes typically entails message transmission between and among cells via small-molecule messengers being funneled from the sender to the receiver cell. Nucleic acids are rarely used as extracellular messengers due to their labile nature and proclivity for enzymatic digestion. Eliminating these obstacles will allow for a larger array of messages to be sent with minimal cellular machinery. Exploiting the bacterial twin-arginine translocation (TAT) pathway and a nucleic-acid binding protein sourced from bacteriophage MS2, we have engineered a message-sending system in Escherichia coli capable of specifically exporting a "pre-written" circularized RNA message to the extracellular environment. This RNA message maintains its integrity over the course of at least four hours in extracellular growth medium, and this system serves as the first demonstration of versatile, stable messaging with nucleic acids, specifically with RNA, in the extracellular environment.
by Lisa Nip.
S.M.
Ghosh, Sumana. "Design And Synthesis Of Novel Interacalator Based Chemical Nuclease." Thesis, Indian Institute of Science, 2001. http://hdl.handle.net/2005/260.
Повний текст джерелаYang, Xiaojuan. "Development of Nanoparticle Systems for Therapeutic Drug Delivery." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1248972068.
Повний текст джерелаTomlinson, Jennifer A. "Nucleic acid-based methods for on-site detection of plant pathogens : approaches and applications." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12957/.
Повний текст джерелаXiao, Linlin. "Detection of Viable Foodborne Pathogens and Spoilage Microorganisms by Nucleic Acid Amplification Based Platforms." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308284180.
Повний текст джерелаBeals, Nathan. "Evaluation of the Delivery and Targeting of Nucleic Acid Based Nanomaterials for Therapeutic Application." Kent State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent1533166304898726.
Повний текст джерелаCanzoneri, Joshua Craig. "Interaction of small molecules with nucleic acid targets: from RNA secondary structure to the riobosome." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/45769.
Повний текст джерелаThompson, Jason Donald. "A syncronous coefficient of drag alteration (SCODA) based technique for sequence specific enrichment of nucleic acids." Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/33073.
Повний текст джерелаLEE, CHEN-CHANG. "Design, Synthesis, and Structure-Bioactivity Characterization of Novel Carbohydrate-Based Polymers as Nucleic Acid Delivery Vectors." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1211919868.
Повний текст джерелаMina, James. "Hyaluronic acid based polymer drug conjugates for the treatment of rheumatoid arthritis." Thesis, University of the West of Scotland, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.739391.
Повний текст джерелаLevy, Maria Patricia Morales. "Development of a DNA vaccine for population control : a zona pellucida based nucleic acid vaccine (ZP-NAV) /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.
Повний текст джерела