Дисертації з теми "Nucleatum"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Nucleatum".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Chen, Jianhe. "Some physiological studies of Fusobacterium nucleatum /." Title page, summary and contents only, 1994. http://web4.library.adelaide.edu.au/theses/09DSM/09dsmc518.pdf.
Повний текст джерелаGravel, Chantal. "Effets de paramètres environnementaux sur Fusobacterium nucleatum." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ38102.pdf.
Повний текст джерелаLee, Felix S. "Amino acid catabolism in Fusobacterium nucleatum and Fusobacterium varium." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0017/MQ57203.pdf.
Повний текст джерелаSuchett-Kaye, George. "Analyse clonale de fusobacterium nucleatum chez des sujets sains." Lyon 1, 1999. http://www.theses.fr/1999LYO1T183.
Повний текст джерелаOgawa, Alice Tayoko. "Caracterização das atividades proteolíticas da bactéria anaeróbia fusobacterium nucleatum." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2004. http://www.bibliotecadigital.uel.br/document/?code=vtls000110059.
Повний текст джерелаThe proteolytic activities of the anaerobe bacillus Fusobacterium nucleatum present in several oral and systemic human infections were determined. The reactions were optimized in their pH, temperature, reaction time, enzyme source and substrate volume. The synthetic substrates b-?naphthylamides (Cys-Na, Ser-Na, Leu-Na, Glu-Na, Lys-Na and BANA), carboxi-L-tyrosine p-nitrophenylester (CTN), and natural substrate azoalbumin were used. With supernatants reaction occurred only with Glu-Na. In the living cells, reacted with Cys-Na, Ser-Na and Glu-Na; and lysate cells with Glu-Na, Leu-Na and CTN. Optimal pH ranged from 6.0 to 7.5 in all the reaction, except for CTN (pH 13). Optimal temperature oscillated between 30 and 40ºC. The optimal reaction time was 60 minutes, except for Glu-Na with living cells (40 min), lysate cells (20 min), and CTN substrate (80 min). There was no activity with azoalbumin. The enzyme activity by several protease inhibitors was assessed and the presence of metallo, serine, cysteine and aspartato protease was detected.
Gunadi, Achmad. "Studies on a peptidase from Fusobacterium nucleatum ATCC 25586 /." Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phg975.pdf.
Повний текст джерелаKiewiet, Paola Thérèse. "The production and characterisation of monoclonal antibodies to fusobacterium nucleatum." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1992. http://hub.hku.hk/bib/B3121082X.
Повний текст джерелаKiewiet, Paola Thérèse. "The production and characterisation of monoclonal antibodies to fusobacterium nucleatum /." Hong Kong : University of Hong Kong, 1992. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13290095.
Повний текст джерелаMidena, Raquel Zanin. "Suscetibilidade antimicrobiana e protocolo de purificação de RNA para análise de expressão gênica de isolados clínicos de Fusobacterium nucleatum." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/25/25147/tde-26022016-143429/.
Повний текст джерелаFusobacterium nucleatum is a Gram-negative bacterial species, strict anaerobic and one of the species often found in primary infection of the root canal system. This species has great importance in biofilm formation to be a union bridge between species which are not able to act alone. The constituent microorganisms of the biofilm exchange each tother genetic material, increasing the strength of them. It is almost impossible for a clinical isolate have genes totally equal to a standard strain, such as strains of culture collections like ATCC (American Type Culture Collection). The present study investigated anaerobic bacterial species Fusobacterium nucleatum isolated from root canals, comparing them to the ATCC strain. The microbial in vitro susceptibility of biofilm and planktonic growth of the strains was compared by means of microbiological culture and the E-test method, with the antibiotics Amoxicillin, Amoxicillin + clavulanic acid, Ampicillin, Azithromycin, Clindamycin, Eritromycin and Metronidazole.. Also, a RNA Purification protocol for the strains under the same growth conditions was defined. Clinical isolates were obtained by microbiological samples of patients with teeth with pulp necrosis and apical periodontitis visible on radiographs. The species isolation and identification were performed using commercial biochemical tests (Sistema Api, Bio-Meriéux, France) and conventional PCR, obtaining four clinical isolates. The protocol for RNA purification was done with zirconia beads, bead beater device and commercial kit RNeasy (Qiagen) and transcribed into complementary DNA (cDNA). The quality of purification was tested for its ability of amplification by real time polymerase chain reaction (qPCR) using primer for the gene 16s RNA specific for F. nucleatum. All tested trains were 100% susceptible to amoxicillin + clavulanic acid, ampicillin, azithromycin, clindamycin and metronidazole. Both types of bacterial growth showed resistance to Erythromycin. Bacteria in biofilm showed a decrease in susceptibility to all antibiotics, but without statistical difference. The protocol proposed for the purification of RNA of F. nucleatum was effective, in the planktonic and the biofilm growth. The average yield of RNA samples for bacteria in planktonic growth was 514.2 ng /μL (SD ± 397.7) and the samples in biofilm was 377.1 ng / μL (SD ± 144.1). These found values suggest a good quality of RNA, free of protein contamination. The established protocol for the purification of the RNA of the Fusobacterium nucleatum strains, grown in biofilm and planktonic phase, had successfully amplified by qPCR.
Darenfed, Hassina. "Activation du plasminogène humain lié à la surface de Fusobacterium nucleatum." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ38063.pdf.
Повний текст джерелаYoo, Christopher Charles. "Investigating the Role of Trimeric Autotransporter Adhesins in Fusobacterium nucleatum Pathogenesis." Thesis, Virginia Tech, 2019. http://hdl.handle.net/10919/101683.
Повний текст джерелаMaster of Science in Life Sciences
Mussi, Maria Carolina Martins. "Análise da atividade antimicrobiana dos óleos de dopaíba (C opaifera officinalis ) e da melaleuca ( Melaleuca alternifolia ) sobre Fusobacterium nucleatum e Por phyromonas gingivalis: determinação das concentrações inibitórias e bactericidas mínimas e efeito de concentrações subinibitórias sobre a agregação." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/25/25150/tde-06122011-101438/.
Повний текст джерелаThe oral cavity is a complex microbial habitat that has more than 500 bacterial species as components of the microbiota. Periodontal health is established when there is equilibrium between pathogens and host. The chlorhexidine digluconate is one of the most commonly used oral antibiotics, however, this substance has been associated with some undesirable side effects. Copaiba and melaleuca oils have been studied as important herbal medicines because of their effects, including antibacterial action. Based on the principle that the copaiba oil and tea tree have an antimicrobial activity and that is no sufficient data in the literature using these herbal medicines against Porphyromonas gingivalis and Fusobacterium nucleatum, Minimum Inhibitory Concentration (MIC) and minimum bactericidal concentration (MBC) tests of Fusobacterium nucleatum (ATCC 25586) and Porphyromonas gingivalis (ATCC 3327) related to chlorhexidine digluconate and oils coming from Copaifera officinalis and Melaleuca alternifolia, were prepared. Assays were performed to determine the subinibitory concentration and the capacity of those bacteria to autoaggregation and coaggregation when exposed to subinibitory concentrations, previously tested. Medium and medium added Tween 80 were used as a control. All oils used had their composition analyzed by gas chromatography-mass spectrometry. The tea tree oil mainly chemical compounds were identified as terpin-4-ol, -terpinen, -terpinen, terpinolene and 1,8-cineole while copaiba oil presented as its main constituents trans-caryophyllene, germacrene B, -humulene, germacrene D and -copaene. The MIC results for F.nucleatum were similar to the CBM data in all solutions. For the bacterium P. gingivalis, all solutions tested inhibited bacterial growth, however, the results obtained during the determination of CBM showed that the copaiba oil was bacteriostatic. All solutions tested inhibited the autoaggregation process but only copaiba oil was effective in inhibiting the coaggregation between F.nucleatum and P. gingivalis. These data suggest that all the solutions tested in this study have relevant changes in the normal development of P.gingivalis and F.nucleatum as well in the influence of the autoaggregation process of F.nucleatum yet Copaiba oil also demonstrated to have remarkable properties to change coaggregation between the bacteria used in this study.
Santos, Thaís Aguiar. "Interação entre Lactobacillus reuteri e bactérias periodontopatogênicas : estudo in vitro e em modelo de invertebrado /." São José dos Campos, 2018. http://hdl.handle.net/11449/166422.
Повний текст джерелаCoorientadora: Liliana Scorzoni
Banca: Cristiane Yumi Koga Ito
Banca: Victor Angelo Martins Montalli
Resumo: A doença periodontal, afecção crônica inflamatória multifatorial, está entre as principais doenças bucais que afetam a população mundial. Entre as bactérias associadas à periodontite, estão Fusobacterium nucleatum e Aggregactibacter actinomycetemcomitans. Novas terapias adjuntas ao tratamento convencional têm sido propostas para a doença periodontal, entre elas o uso de probióticos. Porém, seu uso não está isento de riscos, e uma alternativa para minimizá-los é inativar os micro-organismos, mantendo suas propriedades benéficas, o que ocorre com os chamados paraprobióticos. Desse modo, são objetivos deste estudo avaliar os efeitos antimicrobianos de L. reuteri vivo, inativado pelo calor e seus produtos sobre F. nucleatum, A. actinomycetemcomitans e sobre as bactérias comensais, Streptococcus mitis e Streptococcus salivarius, além de estudar os efeitos da interação das preparações e periodontopatógenos em modelo de invertebrado. A atividade antimicrobiana in vitro foi avaliada associando-se as bactérias patogênicas ou comensais a L. reuteri vivo, inativado ou sobrenadante. Após interação, as bactérias foram cultivadas em meio seletivo para contagem de unidades formadoras de colônias (UFC). No estudo em Galleria mellonella, após a infecção com as bactérias patogênicas e as preparações de L. reuteri, foi avaliada a curva de sobrevivência e densidade hemocitária. Os dados foram analisados com o teste estatístico apropriado, ao nível de 5%. Após interação bacteriana in vitro, S. sa... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Periodontal disease, a chronic multifactorial inflammatory disease, is among the major oral diseases that affects the worldwide population. Among the bacteria associated with periodontitis are Fusobacterium nucleatum and Aggregactibacter actinomycetemcomitans. New therapies have been proposed for periodontal disease as adjunct to conventional treatment, including the use of probiotics. However, their use isn't risk-free and an alternative to that is the inactivation of the microorganisms, maintaining their beneficial properties, which occurs with the paraprobiotics. Thus, the objective of this study is to evaluate the antimicrobial effects of living and heat-killed L. reuteri, and its products on F. nucleatum, A. actinomycetemcomitans and on the commensal bacteria, Streptococcus mitis and Streptococcus salivarius, as well as to study the interaction of the preparations and periodontopathogens in an invertebrate model. In vitro antimicrobial activity was evaluated by associating the pathogenic or commensal bacteria to live, heat killed or L. reuteri supernatant. After interaction, the bacteria were cultured in a selective medium for colony-forming unit (CFU) count. In the study with Galleria mellonella, after infection with pathogenic bacteria and L. reuteri preparations, the survival curve and hemocyte density were evaluated. The data were analyzed with the appropriate statistical test at the 5% level. After bacterial interaction in vitro, S. salivarius reduced the number of ... (Complete abstract click electronic access below)
Mestre
Rodrigues, Viviane Aparecida Arenas. "Avaliação qualitativa, quantitativa e genotípica de Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum isolados de pacientes com diferentes condições clínicas bucais." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-26082015-191740/.
Повний текст джерелаAggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are gram-negative microorganisms observed in inflammatory processes and different forms of periodontal disease. Both are part of the human oral resident microbiota which may cause endogenous or exogenous infections. In this study, a qualitative, quantitative and genotypic analysis of A. actinomycetemcomitans and F. nucleatum isolated from patients with gingivitis, chronic periodontitis and healthy subjects were determined. Subgingival biofilms of 70 patients with gingivitis, 75 with chronic periodontitis and 95 healthy subjects were evaluated. A. actinomycetemcomitans was isolated in 2 (2,8%) patients with gingivitis, 4 (5,3%) with periodontitis and 5 (5,3%) healthy individuals; and F. nucleatum in 13 (18,6%) patients with gingivitis, 20 (26,6%) with chronic periodontitis and 19 (20%) healthy. Both microorganisms were identified in 5 (7,1%) patients with gingivitis, 9 (12%) with chronic periodontitis and 3 (3,15%) healthy. By PCR, DNA of A. actinomycetemcomitans were detected in 23 (32,8%) patients with gingivitis, 20 (26,6%) with chronic periodontitis and 38 (40%) healthy individuals; and F. nucleatum 17 (24,3%) patients with gingivitis, 11 (14,6%) with periodontitis and 19 (20%) healthy. In association, microorganisms were detected in 23 (32,8%) patients with gingivitis, 40 (53,3%) with chronic periodontitis and 17 (17,8%) healthy. A. actinomycetemcomitans isolated from patients with gingivitis belonged to biotype I, II, IV, V, X, and serotypes a, c and e. In patients with periodontitis biotypes II, VI and X and serotypes a, b, and c were found and serotype c was the most predominant (80%); and healthy individuals biotypes II and X, and serotypes b and c. Quantitative values for A. actinomycetemcomitans in the three patients groups were ranged from 0 to 1.14 x 108 and F. nucleatum 0 to 3.98 x 106. The results of this study by AP-PCR showed the heterogeneity of A. actinomycetemcomitans and F. nucleatum in the different clinical status. These comparative results can be considered by dentists for the treatment of periodontal disease and its effective monitoring.
Vasstrand, Endre N. "The rigid cell wall layer of Fusobacterium nucleatum Fev1 an enzymatic and chemical analysis /." Bergen : A/S West-Print, 1986. http://catalog.hathitrust.org/api/volumes/oclc/48051225.html.
Повний текст джерелаEl, Kaddouri Saïd. "Implication de Fusobacterium Nucleatum dans les maladies parodontales humaines : etude de la variabilité intra-espace." Toulouse 3, 2002. http://www.theses.fr/2002TOU30194.
Повний текст джерелаTavares, Lívia Jacovassi. "Eficácia da terapia fotodinâmica antimicrobiana associada ao metronidazol em biofilmes de Fusobacterium nucleatum e Porphyromonas gingivalis /." Araraquara, 2017. http://hdl.handle.net/11449/153496.
Повний текст джерелаResumo: O objetivo deste estudo foi avaliar a eficácia da terapia fotodinâmica antimicrobiana associada (aPDT) ao metronidazol (MTZ) em biofilmes periodontopatogênicos. Para tal finalidade, foram realizadas as seguintes etapas: (1) determinação do tempo de adesão (24 e 48 horas) e formação de biofilme mono e duo-espécie (3, 5 e 7 dias) de Fusobacterium nucleatum (NCTC 11326) e Porphyromonas gingivalis (ATCC 33277); (2) aplicação da aPDT mediada por PDZ associada ao MTZ em biofilmes mono-espécie de F. nucleatum e P. gingivalis. Foram avaliadas diferentes concentrações do PDZ (50, 75 e 100 mg/L) e dose de luz de 50 J/cm2 (660nm). Após a aplicação da aPDT, os biofilmes foram incubados com diferentes concentrações do MTZ (MIC, 50x MIC e 100x MIC) por 24 horas. Os grupos controles positivos (L-F-) não receberam fotossensibilizador e não foram iluminados. A viabilidade dos microrganismos após os tratamentos foi avaliada por meio da contagem de UFC/ml. Os resultados demonstraram que o período de adesão de 24 horas, seguido de 5 dias de formação de biofilme foi satisfatório para a obtenção de biofilmes maduros mono-espécie. Para F. nucleatum, os resultados demonstraram que aPDT 75 mg/mL associado com MTZ 100x MIC e aPDT 100 mg/L associado com MTZ nas concentrações de 50x MIC e 100x MIC reduziu significativamente o número de UFC/mL, 2,99; 2,9 e 3,94 Log10 respectivamente. Para P. gingivalis, a redução mais significativa de UFC/mL foi obtida quando a associação de aPDT 100 mg/L e MTZ 100x MIC ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of this study was to evaluate the efficacy of metronidazole (MTZ) associated antimicrobial photodynamic therapy (aPDT) on periodontopathogenic biofilms. For this purpose, the following steps were performed: (1) determination of adhesion period (24 and 48 hours) and single and duo species biofilm formation (3, 5 and 7 days) of Fusobacterium nucleatum (NCTC 11326) and Porphyromonas gingivalis (ATCC 33277); (2) Photodithazine ® (PDZ)- mediated aPDT in association with MTZ in single-specie biofilms of F. nucleatum and P. gingivalis. Different concentrations of PDZ (50, 75 e 100 mg/L) and light dose of 50 J / cm2 (660nm) were evaluated. After application of aPDT, the biofilms were incubated with different concentrations of MTZ (MIC, 50x MIC and 100x MIC) for 24 hours. Positive control groups (L-F-) received no photosensitizer and were also not illuminated. The viability of the microorganisms after the treatments was evaluated by counting CFU/ml. The results demonstrated that the 24 hours adhesion period followed by 5 days of biofilm formation was satisfactory for obtaining a mature biofilm in single-specie. For F. nucleatum, the results demonstrated that 75 mg/L aPDT associated with MTZ 100x and 100 mg/mL aPDT associated with MTZ at 50x MIC and 100x MIC concentrations significantly reduced the number of CFU/mL, 2.99; 2.9 and 3.94 Log10 respectively. For P. gingivalis, the greatest reduction of CFU/mL was obtained when the association of aPDT 100 mg/L and MTZ 100x MIC was p... (Complete abstract click electronic access below)
Doutor
Souza, Filho Job Alves de. "Aspectos morfológicos, bioquímicos, fisiológicos e moleculares da resposta de Fusobacterium nucleatum a concentrações subinibitórias de antimicrobianos." Universidade Federal de Juiz de Fora (UFJF), 2011. https://repositorio.ufjf.br/jspui/handle/ufjf/2447.
Повний текст джерелаApproved for entry into archive by Diamantino Mayra (mayra.diamantino@ufjf.edu.br) on 2016-09-13T12:46:33Z (GMT) No. of bitstreams: 1 jobalvesdesouzafilho.pdf: 5002481 bytes, checksum: 64848c1386c2e939797e2ac86f5eddd4 (MD5)
Made available in DSpace on 2016-09-13T12:46:33Z (GMT). No. of bitstreams: 1 jobalvesdesouzafilho.pdf: 5002481 bytes, checksum: 64848c1386c2e939797e2ac86f5eddd4 (MD5) Previous issue date: 2011-11-04
FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Concentrações subinibitórias de antimicrobianos (CSI), frequentemente decorrentes de antibioticoterapia, podem resultar em alterações na biologia bacteriana, com implicações no seu potencial agressor. Esse efeito tem considerável importância para as bactérias da microbiota residente, em especial para Fusobacterium nucleatum, um dos mais proeminentes anaeróbios residentes em humanos. Os objetivos deste trabalho foram analisar os efeitos de concentrações subinibitórias (CSI) de antimicrobianos em características morfológicas, bioquímicas, fisiológicas e moleculares de F. nucleatum. A partir da linhagem F. nucleatum ATCC 25586 (denominada FnPAR), foram obtidas 14 linhagens selecionadas por 10 cultivos sucessivos em CSI de ampicilina (FnAMP+), ampicilina/sulbactam (FnAMS+), clindamicina (FnCLI+), cloranfenicol (FnCLO+), levofloxacina (FnLEV+), metronidazol (FnMET+) e piperacilina/tazobactam (FnPTZ+) e, subsequente, 10 cultivos na ausência das mesmas drogas. Foram avaliados o perfil de susceptibilidade aos antimicrobianos, a morfologia bacteriana, o perfil bioquímico, a formação de biofilme e o custo de fitness. Também foram realizados genotipagem e análise dos perfis protéicos. Para avaliação global das alterações fenotípicas e genotípicas foram obtidas matrizes de similaridade. O perfil de susceptibilidade mostrou sensibilidade diminuída para a maioria das linhagens derivadas, mesmo após o cultivo sem drogas. Alterações morfológicas e de complexidade celular foram observadas, principalmente nas linhagens cultivadas em CSI de β-lactâmicos (FnAMP+, FnAMS+ e FnPTZ+), que também expressaram capacidade diminuída para formação de biofilme. Contudo, a morfologia regular e a habilidade de formação de biofilme foram retomadas após o cultivo sem droga. As linhagens FnCLI, FnCLO, FnLEV e FnMET não apresentaram alterações morfológicas evidentes, porém, foi observado aumento na formação de biofilme, com destaque para FnCLI+. As linhagens FnMET+ e FnCLI+ apresentaram um alto custo de fitness. Foram observadas alterações no padrão de metabolismo de carboidratos e na atividade de enzimas microbianas. Comparado com a FnPAR, várias proteínas (de 4.5 a 240 kDa) foram positivamente ou negativamente reguladas nas linhagens derivadas. Observou-se polimorfismo no DNA em todas as linhagens derivadas. As matrizes de similaridade não mostraram relações entre o padrão de polimorfismo de DNA e as outras características. Entretanto, existe uma tendência de que as alterações bioquímicas estejam relacionadas com alterações nos perfis protéicos. CSI de antimicrobianos podem, de fato, induzir alterações em F. nucleatum, com reflexo direto na sua biologia. Estes resultados alertam para o risco de antibioticoterapia inadequada, que podem ter sérias implicações para a microbiologia clinica e doenças infecciosas e, ainda, interferir com a relação bactéria-hospedeiro.
Subinibitory Concentrations (SIC) of antimicrobials, often arising from antibioticotherapy, may result in alterations in bacterial biology with implications for its potential aggressor. This effect has considerable importance for the bacteria of resident microbiota, especially for Fusobacterium nucleatum, one of the most prominent anaerobes in humans. Our aim was to analyze the effects of antimicrobials SIC in morphological, biochemical, physiological and molecular characteristics of F. nucleatum. From the strain F. nucleatum ATCC 25586 (FnWLD) were obtained 14 strains selected by 10 successive culture on SIC of ampicillin (FnAMP+), ampicillin/sulbactam (FnAMS+), clindamycin (FnCLI+), chloramphenicol (FnCLO+), levofloxacin (FnLEV+), metronidazole (FnMET+) and piperacillin/tazobactam (FnPTZ+) and subsequent 10 cultures in the absence of the same drugs. We evaluated the antimicrobial susceptibility patterns, bacterial morphology, biochemical profile, biofilm formation and the fitness cost. We also performed genotyping and analysis of protein profiles. For the global evaluation of phenotypic and genotypic alterations, similarity matrices were obtained. The antimicrobial susceptibility patterns showed decreased sensitivity to most of derived strains, even after culture without drugs. Morphological and cell complexity alterations were observed, mainly in strains grown in SIC of β-lactam (FnAMP+, FnAMS+ and FnPTZ+), which also expressed decreased ability to biofilm formation. However, the regular morphology and the ability to biofilm formation were restored after culture without drug. The strains FnCLI, FnCLO, FnLEV and FnMET showed no apparent morphological changes, however, there was an increase in biofilm formation, especially for FnCLI+. The strains FnMET+ and FnCLI+ had a high fitness cost. Changes were observed in the carbohydrate metabolism patterns and activity of microbial enzymes. Compared with the FnWLD, several proteins (from 4.5 to 240 kDa) were positively or negatively regulated in the derived strains. It was observed polymorphism in the DNA in all derived strains. The similarity matrices showed no relationship between the DNA polymorphism patterns and other features. However, there is a tendency that the biochemical changes to be related to alterations in protein profiles. SIC of antimicrobials may, indeed, to induce alterations in F. nucleatum with direct impact on its biology. These results emphasize the risk of inadequate antibioticotherapy, which may have serious implications for the clinical microbiology and infectious diseases and also to interfere with the bacteria-host relationship.
Mahtout, Hayette. "Interactions des bactéries parodontopathogènes avec les protéines régulatrices du complément." Thesis, Université Laval, 2012. http://www.theses.ulaval.ca/2012/28546/28546.pdf.
Повний текст джерелаPereira, Andressa Lamari Silva. "Avaliação da expressão gênica de citocinas em resposta à infecção experimental por fusobacterium nucleatum e enterococcus faecalis." Universidade Federal de Minas Gerais, 2011. http://hdl.handle.net/1843/ZMRO-8JXQFM.
Повний текст джерелаO objetivo deste estudo foi avaliar a expressão gênica das citocinas pró-inflamatórias (RANKL, TNF- e IFN-) e regulatórias (TGF- e IL-10) em resposta à infecção experimental em molares de camundongos Balb/c com os microrganismos Fusobacterium nucleatum (ATCC 10953) e Enterococcus faecalis (ATCC 19433), em mono ou em bi-associação. Os animais foram sacrificados após 10 e 20 dias, e os tecidos periapicais coletados, dosando-se a presença das citocinas por PCR em tempo real. Observou-se que a mono-infecção com o F. nucleatum induziu, no 10º dia, alta expressão de RANKL e TNF- e que a modulação dessa resposta se deveu à IL-10. O E. faecalis provocou uma alta expressão de IFN- no 20º dia, mas essa modulação observada na expressão do RANKL e TNF- foi independente da IL-10 e TGF-. A bi-associação (F. nucleatum e E. faecalis) estimulou uma alta expressão das citocinas RANKL, TNF- e IFN-, no 10º dia, que seria modulada pela presença aumentada do TGF-. Pode-se concluir que, neste modelo, a expressão gênica das citocinas pró-inflamatórias prevalece nos períodos iniciais de indução das alterações periapicais com concomitante redução no período tardio e isto se deve à modulação promovida pelas citocinas regulatórias IL-10 e TGF-, mas de uma maneira infecção-específica.
Proença, Marcela Alcântara. "Avaliação da influência de Fusobacterium nucleatum na modulação de citocinas e microRNAs em adenoma e câncer colorretal /." São José do Rio Preto, 2017. http://hdl.handle.net/11449/150007.
Повний текст джерелаCoorientador: David J. Hughes
Banca: Marcelo Lima Ribeiro
Banca: Rui Manuel Vieira Reis
Banca: Débora Aparecida Pires de Campos Zuccari
Banca: Marilia De Freitas Calmon Saiki
Resumo: O câncer colorretal (CCR) está associado à patógenos como Fusobacterium nucleatum (Fn), que podem proporcionar um microambiente favorável para a tumorigênese em decorrência de alterações inflamatórias. Visando compreender o efeito de Fn no microambiente de lesões intestinais, avaliou-se a quantificação relativa (RQ) dessa bactéria em amostras de tecido de adenoma colorretal (ACR) e CCR, bem como sua correlação com a expressão de RNAm de mediadores inflamatórios (TLR2, TLR4, NFKB1, TNF, IL1B, IL6 e IL8) e de microRNAs (miRNAs) (miR-21-3p, miR-22-3p, miR-28-5p, miR-34a-5p e miR-135b-5p) envolvidos na resposta inflamatória e carcinogênese. Também delineou-se uma rede de interação miRNA:RNAm para auxiliar na compreensão da participação dos miRNAs no processo carcinogênico. Foram extraídos o DNA e o RNA de 27 amostras de tecido fresco de ACR e 43 de CCR e suas respectivas normais adjacentes. Os níveis de DNA de Fn e de RNAm dos mediadores inflamatórios e miRNAs foram quantificados por PCR quantitativo em tempo real (qPCR). Níveis elevados de Fn foram detectados em ACR (RQ=5,64) e mais acentuadamente em CCR (RQ=8,67). Observou-se expressão elevada do RNAm de TLR4, IL1B, IL8 e miR-135b em ACR, e de TLR2, IL1B, IL6, IL8, miR-34a e miR-135b em CCR em comparação com seus respectivos tecidos normais. Além disso, miR-22 e miR-28 foram encontrados com expressão reduzida em CCR. A expressão de RNAm de IL1B, IL6, IL8 e miR-22 foi positivamente correlacionada com a quantificação de Fn em CCR...
Abstract: Colorectal cancer (CRC) is associated with pathogens such as Fusobacterium nucleatum (Fn), which can provide a favorable microenvironment for tumorigenesis due to inflammatory changes. In order to understand the effect of Fn on the microenvironment of intestinal lesions, the relative quantification (RQ) of this bacterium was evaluated in samples of colorectal adenoma tissue (CRA) and CCR, as well as its correlation with the mRNA expression of inflammatory mediators (TLR2, TLR4, NFKB1, TNF, IL1B, IL6 e IL8), and microRNAs (miR-21-3p, miR-22-3p, miR-28-5p, miR-34a-5p e miR-135b-5p) involved in the inflammatory response and carcinogenesis. A miRNA: mRNA interaction network was also delineated to aid in the understanding of miRNA participation in the carcinogenic process. DNA and RNA were extracted from 27 fresh tissue samples of CRA and 43 of CRC and their respective adjacent normal ones. Fn and mRNA levels of the inflammatory mediators and miRNAs were quantified by quantitative real-time PCR (qPCR). Elevated levels of Fn were detected in CRA (RQ=5.64 and more markedly in CRC (RQ=8.67). High mRNA expression of TLR4, IL1B, IL8 and miR-135b in CRA, and of TLR2, IL1B, IL6, IL8, miR-34a and miR-135b in CRC were observed in comparison with their respective normal tissues. In addition, miR-22 and miR-28 were found downregulated in CRC. The mRNA expression of IL1B, IL6, IL8 and miR-22 was positively correlated with the quantification of Fn in CRC. The mRNA expression of miR-135b and ...
Doutor
Butt, Julia Anna [Verfasser], and Michael [Akademischer Betreuer] Pawlita. "Seroepidemiology of Streptococcus gallolyticus subspecies gallolyticus and Fusobacterium nucleatum with colorectal cancer / Julia Anna Butt ; Betreuer: Michael Pawlita." Heidelberg : Universitätsbibliothek Heidelberg, 2017. http://d-nb.info/1180986148/34.
Повний текст джерелаButt, Julia [Verfasser], and Michael [Akademischer Betreuer] Pawlita. "Seroepidemiology of Streptococcus gallolyticus subspecies gallolyticus and Fusobacterium nucleatum with colorectal cancer / Julia Anna Butt ; Betreuer: Michael Pawlita." Heidelberg : Universitätsbibliothek Heidelberg, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:16-heidok-230806.
Повний текст джерелаCasasanta, Michael Anthony. "Laying the Genetic and Molecular Foundation for the Study of Fusobacterium Nucleatum in Relation to Human Health and Disease." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/88484.
Повний текст джерелаDoctor of Philosophy
Hannu, Olof, and Leonardo Hagman. "Utvärdering av Copan EswabTM för viabilitet av bakterier." Thesis, Hälsohögskolan, Högskolan i Jönköping, HHJ, Avd. för naturvetenskap och biomedicin, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-36170.
Повний текст джерелаBacteria have always had a great influence on mankind. To diagnose any bacterial disease and treat it it’s necessary to identify the bacteria or any relevant attributes. Different types of specimen transport have been developed to keep the bacteria alive from sampling until the analysis is performed. The purpose of the study was to evaluate the viability of bacteria in the fluid-based media Copan EswabTM compared with charcoal medium (Copan swab). Bacteria included in the study were: Campylobacter jejuni, Streptococcus pneumoniae, Haemophilus influenzae, Niesseria gonorrhoeae and Fusobacterium nucleatum. The study also tried to compare how bacteria survived in Eswab which was refrigerated and in Eswab room temperature. Results for H. influenzae (n=9) and N. gonorrhoeae (n=9) showed that an equal amount or more of the bacteria survived in Eswab. More of F. nucleatum (n=9) survived in Copan swab (Copan swab sticks) for the first 28 hours, additionally they showed that the bacteria won’t survive more than 28 hours in room temperature. Regarding S. pneumoniae (n=9) and C. jejuni (n=9) both displayed unreliable results. Overall more measurements and additional studies are needed for more reliable results.
Morin, Marie-Pierre. "Les polyphénols du thé vert : des molécules à double action contre la maladie parodontale." Master's thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26448.
Повний текст джерелаTremblay, Guillaume. "Analyses des corrélations entre les concentrations d'IL-18 et de TLR4 soluble et la charge en bactéries parodontopathogènes chez les patients atteints de parodontite chronique." Master's thesis, Université Laval, 2014. http://hdl.handle.net/20.500.11794/25228.
Повний текст джерелаBravo, Jaimes Sheyla Marilis. "Efectos antibacterianos de las combinaciones alternativas de la droga 3mix y mp sobre bacterias prevalentes en necrosis pulpar." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2015. https://hdl.handle.net/20.500.12672/4459.
Повний текст джерелаTesis
Ariza, Villanueva Carola Maria. "Identificación del Fusobacterium nucleatum en conductos radiculares de dientes deciduos con necrosis pulpar y lesiones periapicales, y su susceptibilidad a la clorhexidina al 0,12%, al 2% e hipoclorito de sodio al 1% y al 5%." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2013. https://hdl.handle.net/20.500.12672/3450.
Повний текст джерелаTesis
Asperger, Michael. "Zur Ätiologie und Bekämpfung der Lumpy Jaw Disease bei Kängurus." Doctoral thesis, Universitätsbibliothek Leipzig, 2004. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-37575.
Повний текст джерелаThe aim of this thesis was the investigation of the aetiology of Lumpy Jaw Disease (LJD) in macropods concentrating specifically on the causes of the diseases in current veterinary medicine literature and to evaluate the use of a group-specific Al(OH)3-adjuvanted, formalin-inactivated whole-cell vaccine for the control of LJD in kangaroos kept in zoos. LJD is regarded as periodontal disease, therefore the risk factors for the development of human periodontitis were also included in this study. The oral flora from 15 healthy macropods and 11 animals suffering from LJD was isolated. At least one anaerobic gram-negative bacterial species was found in swabs of each macropod. The occurrence of Fusobacterium nucleatum was associated with LJD (P < 0.05) by detecting this bacterium in 82% of the kangaroos suffering from LJD compared to only in 33% of the healthy animals. Prevotella oris/oralis and Capnocytophaga spp. were also predominantly found in diseased animals in comparison with healthy macropods (73% vs. 40% and 45% vs. 13% respectively). Bacteroides spp. and Porphyromonas gingivalis were isolated in only 3 and 2 kangaroos suffering from LJD, respectively. Contrary to previously published studies about LJD Fusobacterium necrophorum was not associated with LJD, as this anaerobe was detected in only 27% of the diseased as well as healthy macropods. Moraxella spp. seem to be a part of the normal oral flora of macropods and was found exclusively in healthy animals. 11 Red-necked Wallabies (Macropus rufogriseus) and 2 Red Kangaroos (Macropus rufus) were immunized with a group-specific Al(OH)3-adjuvanted, formalin-inactivated whole-cell vaccine containing previously in a kangaroo suffering from LJD isolated gramnegative anaerobs. The kangaroos were re-vaccinated after 1, 2, 6 and 12 months. Blood was collected from each animal at the same time. Antibodies were titrated against Fusobacterium necrophorum in an agglutination assay. The vaccine failed to induce increased levels of antibodies as well as to protect wallabies and kangaroos against LJD. As the highest antibody titres were detected in most severely diseased wallabies kept in the Hoyerswerda zoo, the protective role of the humoral immune response in LJD seems to be doubtful. The finding of detectable levels of antibodies in unvaccinated joeys supports the theory, that there is a transmission of antibodies from the mother to the offspring via colostrum or yolk-sac placenta. The diet of the Red-necked Wallabies in one zoo has induced an acidosis: The pH of the forestomach fluid collected by probang was lower in the animals of this zoo (pH = 7.53) than in the wallabies of two other zoos (pH = 8.25 and 8.38, respectively). Potassium, cholesterol and -amylase were also higher in the blood of the animals of this zoo in comparison to the wallabies of the two other ones, hence these blood values seem to be helpful for the diagnosis of chronic acidosis in macropods. There was a calcium and phosphor deficiency in the nutrition of the wallabies in two zoos, but the blood concentration of both of these minerals was not changed. The activity of the ALP correlated negative with the age of the Bennett`s Wallabies (P < 0.001, r = -.77 and r = -.62 respectively, depending on the instruments). All of the above mentioned blood values showed no differences between healthy and diseased animals and could so far not support the assumption, that an imbalance in Ca and P metabolism or an acidosis are important factors for LJD. The macropods of all investigated zoos were fed on a diet rich in vitamin A ranging from the 3.5 to the 41fold requirement for lambs. The vitamin A content of the diets for the 2 collections without a history of LJD was the lowest in this study. These results raised the point, that a hypervitaminosis A could be a more predisposing factor for LJD than a vitamin A deficiency. Due to the fact the plasma retinol concentration was independent from the vitamin A content of the diet and so not helpful in diagnosis of a vitamin A deficiency or toxicity, further investigations regarding the role of vitamin A in the aetiopathogenesis of LJD should include measurements of the liver tissue content of retinol esters. The glucose plasma concentration of the healthy Red Kangaroos (8.57 mmol/l) as well as the Red-necked Wallabies (6.51 mmol/l) was higher than previously published values for macropods, but also higher than the results of the diseased animals in this study. Therefore diabetes mellitus can be ruled out as an underlying factor for LJD. The analysis of 144 pathological records showed, that 30 animals died because of LJD, 20% of them and 16.7% of the other 114 macropods had a concurrent kidney disease. The urea and creatinin concentration in serum samples of healthy animals was not higher than the values of diseased animals. In conclusion, these results suggest kidney diseases are not important for the development of LJD. Altogether 184 sera collected from 107 kangaroos were tested for antibodies against MaHV-1 and MaHV-2 using a neutralisation assay. The prevalence of the MaHV-1- as well as MaHV-2-antibodies was high among the Red Kangaroos (94.4% and 97.2% respectively), but low among the Red-necked Wallabies (5.6% and 4.2% respectively). Seroconversion for MaHV-1 was seen in 2 out of 21 wallabies suffering from LJD, only 1 of these animals also had antibodies against MaHV-2. The antibody-titres against both of the macropodid herpes viruses also did not differ between Red Kangaroos with and without LJD, therefore a reactivation of a latent herpesvirus infection does not appear to be causative for LJD. In summary, considering the results of this study and previously published literature LJD is an infectious disease caused by gramnegative anaerobic bacteria with Fusobacterium nucleatum, Bacteroides spp., Porphyromonas gingivalis and Fusobacterium necrophorum subsp. necrophorum being of most significance. Recommendations concerning the keeping of kangaroos in captivity and the management of LJD are listed in the conclusion of this thesis. Some radiographs and photos of diseased and healthy kangaroos are attached
Tiang, Jen. "Shear-induced crystallization of nucleated polymers." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92255.
Повний текст джерелаLa cristallisation sous ou après un cisaillement fort est importante dans les procédés de mise en forme des polymères dus à ses effets significatifs sur les propriétés du produit et sur le taux de production. Cette étude traite de plusieurs importantes lacunes dans notre connaissance de ce phénomène en étudiant la cristallisation induite par cisaillement des polypropylènes isotactiques contenant un agent nucléant par une mesure simultanée de l'intensité lumineuse et de la rhéologie en utilisant un rhéomètre à plaques parallèles à des taux de cisaillement élevés et homogènes. Une sonde à fibres optiques a été conçue pour mesurer l'intensité lumineuse. Pour les conditions étudiées, ces deux mesures se sont avérées être aussi bien en mesure de détecter des structures cristallines naissantes, mais l'intensité lumineuse est mieux adaptée pour surveiller les changements au cours des premiers stades de la cristallisation, considérant que la rhéologie est plus utile pour en suivre les derniers stades. L'influence relative d'un agent nucléant ne réagissant pas à la fusion, le poids moléculaire, le taux et la déformation du cisaillement ont été étudiés en conditions isothermes avec faible surfusion après un cisaillement bref. Si on la compare à une cristallisation en condition statique, la voie de la germination des polymères nucléés suite à un fort cisaillement est apparue être régie non pas par l'agent nucléant, mais par le poids moléculaire. Le principal effet du cisaillement a été confirmé être présent durant la germination induite et est beaucoup plus faible en changeant la cinétique de croissance. Le cisaillement et l'agent nucléant ont à la fois réduit le temps d'induction, mais les effets se sont révélés ne pas être cumulatifs. Avec l'augmentation du taux de cisaillement ou de la déformation, la cristallisation s'est avérée tout d'abord accélérée comme une conséquence de l'augmentation du
Kennedy, Mary A. "Crystallization and melting behavior studies of un-nucleated and silica-nucleated isotactic polystyrene and isotactic poly(propylene oxide)." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75765.
Повний текст джерелаThe surface morphologies and growth rates of i-PS spherulites, as studied by photomicroscopy, were not affected by 1 pph of silica. The experimental data were fitted to a modified form of the Hoffman-Lauritzen equation.
The effect of silica on spherulite growth rates and surface morphologies of isotactic poly(propylene oxide) (i-PPO) have also been investigated by optical microscopy. Two distinct i-PPO samples of different molecular weights were used, each of which was highly isotactic. The addition of silica has a pronounced effect on the morphology of the spherulites, producing dendritic type morphology. Upon step-crystallization, the spherulites exhibited mixed morphologies, i.e., fibrillar and ringed. Silica depresses the spherulite growth rates throughout the entire temperature range. The effects were more profound as the quantity of filler increased. The growth rate-temperature behavior was analysed in terms of the classical Hoffman-Lauritzen equation and a modified version to take into account the polymer-filler interaction.
Pascual, Christopher C. "EHD enhancement of nucleate pool boiling." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/19027.
Повний текст джерелаOslick, Sherri L. (Sherri Lynn). "Characterization of short template-nucleated helical peptides." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/38779.
Повний текст джерелаMichta, Edouard. "Modeling of Subcooled Nucleate Boiling with OpenFOAM." Thesis, KTH, Reaktorteknologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-32042.
Повний текст джерелаBreen, R. J. "PWR safety studies : nucleate boiling heat transfer." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236258.
Повний текст джерелаBertsch, George M., Stephen B. Memory, and P. J. Marto. "Nucleate pool boiling characteristics of R-124." Thesis, Monterey, California: Naval Postgraduate School, 1993. http://hdl.handle.net/10945/24202.
Повний текст джерелаPoon, Steven Sui-Sang. "Algorithms for detecting and segmenting nucleated blood cells." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27989.
Повний текст джерелаApplied Science, Faculty of
Electrical and Computer Engineering, Department of
Graduate
Miti, Tatiana. "Thermodynamic and Kinetic Aspects of Hen Egg White Lysozyme Amyloid Assembly." Scholar Commons, 2017. https://scholarcommons.usf.edu/etd/7425.
Повний текст джерелаArata, Frank A. "Nucleate boiling heat transfer study of direct immersion cooling of a 3x3 array of vertically orientated electronic components in a dielectric fluid." Thesis, Monterey, California : Naval Postgraduate School, 1992. http://handle.dtic.mil/100.2/ADA257558.
Повний текст джерелаThesis Advisor: Kelleher, M. D. "September 1992." Description based on title screen as viewed on April 16, 2009. Includes bibliographical references (p. 92). Also available in print.
Sarmento, Carlos Alberto Palmeira. "Estimativa do número de cardiomiócitos do ventrículo esquerdo em suínos (Sus scrofa)." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-26112009-103842/.
Повний текст джерелаInvestigations involving the heart have been largely carried out all over the years due to its importance in medicine. Heart diseases are the one of the major causes of deaths and hospitalizations in Brazil and worldwide. In this context, technological innovations and progresses in methodological investigation have been frequently proposed; Swine has been shown as a useful model due to similarities to human. The application of stereological tools for investigations in the morphology of the heart has been intensely applied based on the reliability on the results for measuring and quantification. This study aimed to evaluate morphological and quantitative aspects of cardiomyocytes in the left ventricle in healthy swine. Three female swine, averaging 25 kg were euthanasiated and have the hearth chambers separated. Left ventricles were fixed and slides obtained. Images were acquired and analyzed by dissector and nucleator. The results showed us that the average weight of the left ventricle of the animals was 660 mg, the mean volume of cardiomyocytes was 16:32 µm3, the average number of cardiomyocytes was 3.91 x108, while the average volume of cardiomyocytes that occupy the left ventricle was 3.32 x106.
Cryer, Matthew A. "An experimental study of high heat flux removal using micro-droplet spray cooling." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2003. http://library.nps.navy.mil/uhtbin/hyperion-image/03Jun%5FCryer.pdf.
Повний текст джерелаDomenicucci, Carmelo. "In search of a protein nucleator of hydroxyapatite in bone." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0005/NQ27912.pdf.
Повний текст джерелаHenry, Christopher Douglas. "Nucleate pool boiling characteristics from a horizontal microheater array." College Park, Md. : University of Maryland, 2005. http://hdl.handle.net/1903/3185.
Повний текст джерелаThesis research directed by: Mechanical Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
Ellepola, Jerome. "Nucleate boiling : nonlinear spatio-temporal variations in wall temperature." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389327.
Повний текст джерелаHolland, Adrian Mark. "Control of nucleate boiling with micro-machined surface features." Thesis, Loughborough University, 2004. https://dspace.lboro.ac.uk/2134/11343.
Повний текст джерелаNeu, Samuel Charles. "Experimental and Computational Investigation of Electrohydrodynamically –Enhanced Nucleate Boiling." Digital WPI, 2016. https://digitalcommons.wpi.edu/etd-dissertations/405.
Повний текст джерелаWasekar, Vivek Mahadeorao. "Nucleate Pool Boiling Heat Transfer in Aqueous Surfactant Solutions." University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin994964318.
Повний текст джерелаBailey, James. "Phenomenological modeling of the nucleated polymerization of human islet amyloid polypeptide : a combined experimental and theoretical approach." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/2441.
Повний текст джерелаKelley, Mitchell Joseph. "Experimental design for study of nucleate boiling in porous structures." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/68530.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (p. 48).
The superheat required to initiate nucleate boiling inside porous wicks is not well understood in practice. This thesis reports the design of an experimental setup for investigating the onset of vapor nucleation in sintered porous structures. Pressure sensing was evaluated as an effective means of detecting the onset of nucleation. Thermal studies were conducted with a custom finite difference script in conjunction with finite element analysis. Heat conduction through a three dimensional wick was reduced to one dimensional conduction via symmetry and design constraints. The wick was optimized to achieve a temperature drop of 30 *C at a common heat pipe operating temperature of 70 °C.
by Mitchell Joseph Kelley.
S.B.