Готові списки джерел за темами / Normothermic Machine Perfusion

Добірка наукової літератури з теми "Normothermic Machine Perfusion"

Автор: Grafiati

Опубліковано: 14 березня 2023

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Статті в журналах з теми "Normothermic Machine Perfusion"

Hann, A., H. Lembach, A. Nutu, B. Dassanayake, S. Tillakaratne, S. C. McKay, A. P. C. S. Boteon, et al. "Outcomes of normothermic machine perfusion of liver grafts in repeat liver transplantation (NAPLES initiative)." British Journal of Surgery 109, no. 4 (February 15, 2022): 372–80. http://dx.doi.org/10.1093/bjs/znab475.

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Abstract Background Retransplantation candidates are disadvantaged owing to lack of good-quality liver grafts. Strategies that can facilitate transplantation of suboptimal grafts into retransplant candidates require investigation. The aim was to determine whether late liver retransplantation can be performed safely with suboptimal grafts, following normothermic machine perfusion. Methods A prospectively enrolled group of patients who required liver retransplantation received a suboptimal graft preserved via normothermic machine perfusion. This group was compared with both historical and contemporaneous cohorts of patient who received grafts preserved by cold storage. The primary outcome was 6-month graft and patient survival. Results The normothermic machine perfusion group comprised 26 patients. The historical (cold storage 1) and contemporaneous (cold storage 2) groups comprised 31 and 25 patients respectively. The 6-month graft survival rate did not differ between groups (cold storage 1, 27 of 31, cold storage 2, 22 of 25; normothermic machine perfusion, 22 of 26; P = 0.934). This was despite the normothermic machine perfusion group having significantly more steatotic grafts (8 of 31, 7 of 25, and 14 of 26 respectively; P = 0.006) and grafts previously declined by at least one other transplant centre (5 of 31, 9 of 25, and 21 of 26; P < 0.001). Conclusion In liver retransplantation, normothermic machine perfusion can safely expand graft options without compromising short-term outcomes.

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Liew, Belle, David Nasralla, Satheesh Iype, Joerg-Matthias Pollok, Brian Davidson, and Dimitri A. Raptis. "Liver transplant outcomes after ex vivo machine perfusion: a meta-analysis." British Journal of Surgery 108, no. 12 (November 17, 2021): 1409–16. http://dx.doi.org/10.1093/bjs/znab364.

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Abstract Background The pressure on liver-transplant programmes has expanded the usage of extended-criteria allografts. Machine perfusion may be better than conventional static cold storage (SCS) in alleviating ischaemia–reperfusion injury in this setting. Recipient outcomes with hypothermic or normothermic machine perfusion were assessed against SCS here. Methods A search in MEDLINE, EMBASE and Scopus was conducted in February 2021. Primary studies investigating ex vivo machine perfusion were assessed for the following outcomes: morbidity, ICU and hospital stay, graft and patient survival rates and relative costs. Meta-analysis was performed to obtain pooled summary measures. Results Thirty-four articles involving 1742 patients were included, of which 20 were used for quantitative synthesis. Odds ratios favoured hypothermic machine perfusion (over SCS) with less early allograft dysfunction, ischaemic cholangiopathy, non-anastomotic strictures and graft loss. Hypothermic machine perfusion was associated with a shorter hospital stay and normothermic machine perfusion with reduced graft injury. Two randomized clinical trials found normothermic machine perfusion reduced major complication risks. Conclusion Machine perfusion assists some outcomes with potential cost savings.

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Goumard, Claire, Célia Turco, Mehdi Sakka, Lynda Aoudjehane, Philippe Lesnik, Eric Savier, Filomena Conti, and Olivier Scatton. "Ex-Vivo Pharmacological Defatting of the Liver: A Review." Journal of Clinical Medicine 10, no. 6 (March 18, 2021): 1253. http://dx.doi.org/10.3390/jcm10061253.

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The ongoing organ shortage has forced transplant teams to develop alternate sources of liver grafts. In this setting, ex-situ machine perfusion has rapidly developed as a promising tool to assess viability and improve the function of organs from extended criteria donors, including fatty liver grafts. In particular, normothermic machine perfusion represents a powerful tool to test a liver in full 37 °C metabolism and add pharmacological corrections whenever needed. In this context, many pharmacological agents and therapeutics have been tested to induce liver defatting on normothermic machine perfusion with promising results even on human organs. This systematic review makes a comprehensive synthesis on existing pharmacological therapies for liver defatting, with special focus on normothermic liver machine perfusion as an experimental ex-vivo translational model.

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Weissenbacher, Annemarie, and James Hunter. "Normothermic machine perfusion of the kidney." Current Opinion in Organ Transplantation 22, no. 6 (December 2017): 571–76. http://dx.doi.org/10.1097/mot.0000000000000470.

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Mergental, Hynek, and Garrett R. Roll. "Normothermic machine perfusion of the liver." Clinical Liver Disease 10, no. 4 (October 2017): 97–99. http://dx.doi.org/10.1002/cld.661.

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Croome, Kristopher P. "Introducing Machine Perfusion into Routine Clinical Practice for Liver Transplantation in the United States: The Moment Has Finally Come." Journal of Clinical Medicine 12, no. 3 (January 23, 2023): 909. http://dx.doi.org/10.3390/jcm12030909.

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While adoption of machine perfusion technologies into clinical practice in the United States has been much slower than in Europe, recent changes in the transplant landscape as well as device availability following FDA approval have paved the way for rapid growth. Machine perfusion may provide one mechanism to maximize the utilization of potential donor liver grafts. Indeed, multiple studies have shown increased organ utilization with the implementation of technologies such as ex-situ normothermic machine perfusion (NMP), ex-situ hypothermic machine perfusion (HMP) and in-situ normothermic regional perfusion (NRP). The current review describes the history and development of machine perfusion utilization in the Unites States along with future directions. It also describes the differences in landscape between Europe and the United States and how this has shaped clinical application of these technologies.

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Prudhomme, Thomas, Delphine Kervella, Stéphanie Le Bas-Bernardet, Diego Cantarovich, Georges Karam, Gilles Blancho, and Julien Branchereau. "Ex situ Perfusion of Pancreas for Whole-Organ Transplantation: Is it Safe and Feasible? A Systematic Review." Journal of Diabetes Science and Technology 14, no. 1 (August 13, 2019): 120–34. http://dx.doi.org/10.1177/1932296819869312.

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Introduction: Pancreas transplantation is currently one of the best treatments proposed in highly selected patients with unstable and brittle type 1 diabetes. The objective of pancreas transplantation is to restore normoglycemia and avoid the occurrence of complications associated with diabetes. Graft pancreatitis and thrombosis, arising from ischemia reperfusion injuries, are major causes of graft loss in the postoperative period. Ex situ perfusion, in hypothermic or normothermic settings, allowed to improve ischemic reperfusion injury in other organ transplantations (kidney, liver, or lung). The development of pancreatic graft perfusion techniques would limit these ischemic reperfusion injuries. Objective: Evaluation of the safety and feasibility of ex situ perfusion of pancreas for whole-organ transplantation. Methods: English literature about pancreas perfusion was analyzed using electronic database Medline via PubMed (1950-2018). Exclusion criteria were studies that did not specify the technical aspects of machine perfusion and studies focused only on pancreas perfusion for islet isolation. Results: Hypothermic machine perfusion for pancreas preservation has been evaluated in nine studies and normothermic machine perfusion in ten studies. We evaluated machine perfusion model, types of experimental model, anatomy, perfusion parameters, flushing and perfusion solution, length of perfusion, and comparison between static cold storage and perfusion. Conclusions: This review compared ex vivo machine perfusion of experimental pancreas for whole-organ transplantation. Pancreas perfusion is feasible and could be a helpful tool to evaluate pancreas prior to transplantation. Pancreas perfusion (in hypothermic or normothermic settings) could reduce ischemic reperfusion injuries, and maybe could avoid pancreas thrombosis and reduce morbidity of pancreas transplantation.

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op den Dries, S., N. Karimian, and R. J. Porte. "Normothermic Machine Perfusion of Discarded Liver Grafts." American Journal of Transplantation 13, no. 9 (September 2013): 2504. http://dx.doi.org/10.1111/ajt.12374.

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Karimian, Negin, Siavash Raigani, Viola Huang, Sonal Nagpal, Ehab O. A. Hafiz, Irene Beijert, Paria Mahboub, et al. "Subnormothermic Machine Perfusion of Steatotic Livers Results in Increased Energy Charge at the Cost of Anti-Oxidant Capacity Compared to Normothermic Perfusion." Metabolites 9, no. 11 (October 24, 2019): 246. http://dx.doi.org/10.3390/metabo9110246.

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There continues to be significant debate regarding the most effective mode of ex situ machine perfusion of livers for transplantation. Subnormothermic (SNMP) and normothermic machine perfusion (NMP) are two methods with different benefits. We examined the metabolomic profiles of discarded steatotic human livers during three hours of subnormothermic or normothermic machine perfusion. Steatotic livers regenerate higher stores of ATP during SNMP than NMP. However, there is a significant depletion of available glutathione during SNMP, likely due to an inability to overcome the high energy threshold needed to synthesize glutathione. This highlights the increased oxidative stress apparent in steatotic livers. Rescue of discarded steatotic livers with machine perfusion may require the optimization of redox status through repletion or supplementation of reducing agents.

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Large, Stephen, and Simon Messer. "Machine Perfusion of the Human Heart." Transplantology 3, no. 1 (March 18, 2022): 109–14. http://dx.doi.org/10.3390/transplantology3010011.

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This brief communication about machine perfusion of potential human donor hearts describes its historical development. Included in the review are both the isolated perfusion of donor hearts retrieved from heart beating and non-heart-beating donors. Additionally, some detail of in-situ (within the donor body) normothermic regional reperfusion of the heart and other organs is given. This only applies to the DCD donor heart. Similarly, some detail of ex-situ (outside the body) heart perfusion is offered. This article covers the entire history of the reperfusion of donor hearts. It takes us up to the current day describing 6 years follow-up of these donor machine perfused hearts. These clinical results appear similar to the outcomes of heart beating donors if reperfusion is managed within 30 min of normothermic circulatory determined death. Future developments are also offered. These are 3-fold and include: i. the pressing need for objective markers of the clinical outcome after transplantation, ii. the wish for isolated heart perfusion leading to improvement in donor heart quality, and iii. a strategy to safely lengthen the duration of isolated heart perfusion.

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Більше джерел

Дисертації з теми "Normothermic Machine Perfusion"

CATALANO, GIORGIA. "Human liver stem cells and derived products in experimental models of liver ischemia reperfusion injury and of liver isolated normothermic perfusion." Doctoral thesis, Politecnico di Torino, 2018. http://hdl.handle.net/11583/2711047.

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Ischemia reperfusion injury (IRI) is an antigen-independent inflammatory event that affects several clinical settings, including surgical procedures such as liver resection and liver transplantation. IRI is still a major concern in the transplantation setting, causing up to 10% of early graft failure and leading to a higher incidence of acute and chronic rejection. IRI is initiated by Kuppfer cells and hepatocytes through a massive production of reactive oxygen species (ROS) during the ischemic phase and, in a major degree, during the post-reperfusion phase. ROS directly damage hepatocytes and endothelial cells and promote the recruiment of neutrophils and T-cells, starting an inflammatory cascade that triggers apoptosis and necrosis. Human Liver Stem Cells (HLSC) have been identified as a population of pluripotent resident liver cells able to express markers characteristic of the mesenchymal lineage (CD73, CD90, CD29, CD44) together with hepatic markers (alpha-fetoprotein, cytokeratin 18, cytokerain 8 and albumin), suggesting a partial hepatic commitment. HLSC share self-renewal properties with mesenchymal stem cells and can actively take part to tissue remodeling and liver regeneration. We found that HLSC are able to localize within the injured tissue and promote liver regeneration in a murine model of fulminant liver failure and to generate a functional “humanized liver-like tissue” when injected in rat acellular liver scaffolds. Growing evidence suggests that the biological effects of stem cells on neighboring cells are mediated by paracrine mechanisms including the release of extracellular vesicles (EV). EV are an heterogeneous population of cell-secreted vesicles originating from the endosomal compartment or from direct budding of plasma membrane that are able to modify the phenotype and function of neighboring cells. The regenerative effects of EV are well documented and ascribed to a horizontal transfer of proteins, lipids and, above all, specific subsets of mRNA and miRNA. In a rat model of 70% hepatectomy, animals treated with Human Liver Stem Cells-derived Extracellular Vesicles (HLSC-EV) revealed a significant reduction of liver injury and higher regeneration rate of the remnant liver after surgery . The effect of HLSC-EV was investigated on two different experimental models: First Project We set up a short-duration model of ex vivo isolated rat liver Normothermic Machine Perfusion (NMP) in which oxygen delivery was kept suboptimal through a low hemoglobin content in the perfusate. In this model, we investigated whether adding HLSC-EV to the circuit could result in i) their rapid uptake by the liver, and ii) an appreciable reduction of the hypoxic tissue injury. Second Project An in-vivo model of IRI was set up in mice. The aim of this study was to investigate the effects of systemic administration of HLSC-EV on tissue injury after partial clamping of the hepatic hilum (70%) .

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Gringeri, Enrico. "Un nuovo sistema di perfusione parenchimale per la preservazione del graft epatico per trapianto: valutazione sperimentale sul piccolo e grande animale." Doctoral thesis, Università degli studi di Padova, 2008. http://hdl.handle.net/11577/3425544.

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Background In liver transplantation one of the most important promblem which has not a solution yet is the large discrepancy between supply and demand for liver transplantation. To expand the pool of organs we should find the way to utilize marginal livers like the Non-heart-beating-donors ones and the fatty grafts. The use of these livers is associated with high incidence of post-operative primary dysfunction caused by the damage due to the Cold Storage preservation in the context of the process of ischaemia- reperfusion in extreme hypothermia (4° C). The utilization of new methods in the conservation of hepatic grafts like Machine Perfusion becomes necessary, especially for its ability to reduce the damage of ischaemia-reperfusion in hypothermia. The great potentiality of this Machine in sane and marginal organ preservation and the number of works which took place about it, stimulated us to the deepen this argument. Purpose We decided to create a experimental model of Machine Perfusion for pigs and to test it with different temperatures to obtain the best condition of perfusion and graft preservation. We based our study on the various works that can be find in literature and on the research developed by the Università di Pavia on rats. Methods We used in our experiments 15 Landrace pig of the weight of 22 kilos each. We divided them in three groups of study. Group C: after hepatectomy the pig liver was preserved in Cold Storage (4° C) for 8 hours. Group N: after hepatectomy the pig liver was perfused in Normothermic Machine Perfusion (37°C) for 8 hours. Group I: the pig liver was perfused in Moderate Hypothermic Machine (20°C). After the preservation, all the 15 livers were tested with a rewarming (37°C) of 2 hours. During the two hours of the experiment we performed samples of blood and biopsies and we also valuated the indocianine green clearance. Results The results obtained by the histology, the functional test and biochemical levels of AST, ALT and LDH, in the moderate hypothermic perfusion prove the superiority of this machine . Conclusion It is concluded after these experiments the great ability of 20°C Machine Perfusion in pig's liver transplantation. We think that this method of preservation will be able to improve the pool of donors using NHBD livers and fatty livers. In future our research will deepen the problematic connected with the preservation of marginal organs, in particular of steatotic livers.

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Allain, Géraldine. "Greffons rénaux issus des donneurs décédés par arrêt circulatoire : optimisation du reconditionnement chez le donneur et de la conservation hypothermique." Thesis, Poitiers, 2018. http://www.theses.fr/2018POIT1410/document.

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La transplantation est la meilleure alternative en cas d'insuffisance rénale terminale. Face à la pénurie de greffons, les équipes de transplantation se sont tournées notamment vers les donneurs décédés par arrêt circulatoire (DDAC) non contrôlés. Ces greffons soumis à une période d'ischémie chaude sont plus fragiles. Des méthodes de reconditionnement chez le donneur par refroidissement in situ (RIS) et circulation régionale normothermique (CRN) se sont développées afin de réduire les lésions d'ischémie-reperfusion. Le choix de la méthode est laissé à l'appréciation de chaque équipe et il existe une grande hétérogénéité des pratiques. Après prélèvement, l'utilisation des machines de perfusion hypothermique (MPH) est généralement recommandée. L'optimisation de ces phases de reconditionnement chez le donneur et de conservation hypothermique apparait comme un enjeu majeur de santé publique. Concernant l'optimisation du mode de reconditionnement, la mise au point d'un modèle préclinique porcin parfaitement reproductible a permis de mettre en évidence une supériorité de la CRN sur le RIS. Une durée de CRN de 4 heures minimum sans dépasser 6 heures paraît optimale. Concernant la conservation hypothermique, les MPH permettent le maintien du niveau d'expression des gènes retrouvé en fin de CRN. L'ajout d'une oxygénation active en MPH ou de curcumine en solution statique améliore le devenir du greffon à court et long termes dans un modèle préclinique d'autogreffe. Ce travail pourrait s'étendre à l'étude d'autres organes, d'autres durées d'ischémie chaude et aux DDAC contrôlés afin d'élargir encore le nombre d'organes éligibles à la transplantation
Transplantation is the best alternative to end-stage renal disease. The shortage of grafts led the transplant teams to consider uncontrolled deceased donors after circulatory death (DCDs). These grafts suffered from a period of warm ischemia and are more vulnerable. Reconditioning methods in the donor by in situ cooling (ISC) and normothermic regional perfusion (NRP) have been developed to reduce the ischemia-reperfusion injuries. Each team has the choice as to the method and there are many different practices. After removal of kidneys, the use of hypothermic perfusion machines (HPM) is generally recommended. The optimization of reconditioning in the donor and hypothermic preservation appears as a major public health challenge. About optimization of the reconditioning method, the development of a high reproducible preclinical porcine model allowed to highlight the superiority of RNP over ISC. NRP duration of 4 hours minimum without exceeding 6 hours seems optimal. About hypothermic preservation, HPM allows to maintain the level of expression of the genes found at the end of RNP. The addition of active oxygenation to HPM or curcumin in static solution improves the graft outcomes in the short and long terms in a preclinical model of auto transplantation. This work could be extended to the study of other organs, other durations of warm ischemia and to controlled DCDs in order to further increase the number of transplantable grafts

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Частини книг з теми "Normothermic Machine Perfusion"

Kumar, G. V. Prem, P. Balachandran, and K. Anusha. "Normothermic Machine Perfusion." In Peri-operative Anesthetic Management in Liver Transplantation, 361–72. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-6045-1_28.

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Pezzati, Daniele, Qiang Liu, and Cristiano Quintini. "Ex Vivo Normothermic Machine Perfusion." In Donation after Circulatory Death (DCD) Liver Transplantation, 217–35. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-46470-7_15.

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Georgiades, Fanourios, Sarah A. Hosgood, and Michael L. Nicholson. "Assessing and reconditioning kidneys using normothermic machine perfusion." In Organ Repair and Regeneration, 75–93. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-819451-5.00011-1.

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"Donor heart procurement: donation after brain death and donation after circulatory determined death." In Cardiopulmonary transplantation and mechanical circulatory support, edited by Maziar Khorsandi, Steven Tsui, John Dark, Alan J. Kirk, Matthew Hartwig, Mani A. Daneshmand, Carmelo Milano, et al., 171–84. Oxford University PressOxford, 2022. http://dx.doi.org/10.1093/med/9780192867612.003.0015.

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Abstract Heart transplantation remains the gold standard treatment for advanced heart failure refractory to medical management. However, the shortage of suitable donor hearts means that the number of patients on the heart transplant waiting list continues to increase. In an effort to satisfy this increasing demand, the margins of donor organ acceptability have been extended to include donor hearts with a history of prolonged cardiac arrest, left ventricular hypertrophy, low ejection function, and, recently, hearts from donation after circulatory determined death donors. In order to safely utilize these extended criteria donor hearts, organ procurement and preservation techniques have had to evolve over time from the traditional cold static storage to include normothermic regional perfusion of the donor and machine perfusion of retrieved donor hearts.

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Тези доповідей конференцій з теми "Normothermic Machine Perfusion"

Kneifel, F., I. Flammang, F. Becker, T. Vogel, S. Radünz, P. Houben, H. Schmidt, A. Pascher, and JG Brockmann. "First year experience of liver graft normothermic machine perfusion at University hospital Münster." In 37. Jahrestagung der Deutschen Arbeitsgemeinschaft zum Studium der Leber. Georg Thieme Verlag KG, 2021. http://dx.doi.org/10.1055/s-0040-1721984.

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Muth, Vanessa, JosephM G. V. Gassner, Julian Michelotto, Felix Strobl, Kristina Knaub, Maximilian Zimmer, Simon Mossburner, Johann Pratschke, IgorM Sauer, and Nathanael Raschzok. "Bile as parameter for quality assessment during normothermic ex vivo rat liver machine perfusion." In 38. Jahrestagung der Deutsche Arbeitsgemeinschaft zum Studium der Leber. Georg Thieme Verlag, 2022. http://dx.doi.org/10.1055/s-0041-1740714.

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Flammang, I., F. Kneifel, F. Becker, P. Houben, S. Radünz, T. Vogel, A. Pascher, and JG Brockmann. "Assessment of liver allograft viability during ex vivo normothermic machine perfusion using the MS2 score." In 37. Jahrestagung der Deutschen Arbeitsgemeinschaft zum Studium der Leber. Georg Thieme Verlag KG, 2021. http://dx.doi.org/10.1055/s-0040-1721985.

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Strobl, Felix, Julian Michelotto, Vanessa Muth, Kristina Knaub, Maximilian Zimmer, Simon Moosburner, Johann Pratschke, IgorM Sauer, Nathanael Raschzok, and JosephM G. V. Gaßner. "Optimising a rodent model of normothermic ex-vivo liver machine perfusion to mimic clinical application." In 38. Jahrestagung der Deutsche Arbeitsgemeinschaft zum Studium der Leber. Georg Thieme Verlag, 2022. http://dx.doi.org/10.1055/s-0041-1740716.

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Звіти організацій з теми "Normothermic Machine Perfusion"

Si, Tengfei, Zhenlin Huang, Salma Mujib, and Yun Ma. Normothermic machine perfusion versus static cold storage for liver graft preservation: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, June 2020. http://dx.doi.org/10.37766/inplasy2020.6.0038.

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