Дисертації з теми "NMR spectroscopy of metabolites"
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Al-Busaidi, Harith N. K. "Secondary metabolites from Xylaria endophytes : the isolation and structure elucidation of secondary metabolites from Xylaria endophytes by chemical and spectroscopic methods." Thesis, University of Bradford, 2011. http://hdl.handle.net/10454/5266.
Повний текст джерелаLenz, Eva-Maria. "Multinuclear NMR and HPLC-NMR spectroscopic studies on xenobiotic metabolism." Thesis, Birkbeck (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267785.
Повний текст джерелаAsllani, Iris. "Anisotropic interactions of metabolites in skeletal muscle observed by dipolar coupling in ¹H NMR spectroscopy /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/8121.
Повний текст джерелаEhlers, Ina. "NMR studies of metabolites and xenobiotics: From time-points to long-term metabolic regulation." Doctoral thesis, Umeå universitet, Institutionen för medicinsk kemi och biofysik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-97684.
Повний текст джерелаManton, David John. "Quantification of brain metabolites by in vivo proton MR spectroscopy : investigations into reproducibility and application to studies of intracranial tumours." Thesis, University of Hull, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321120.
Повний текст джерелаTolonen, A. (Ari). "Analysis of secondary metabolites in plant and cell culture tissue of Hypericum perforatum L and Rhodiola rosea L." Doctoral thesis, University of Oulu, 2003. http://urn.fi/urn:isbn:9514271610.
Повний текст джерелаAl-Busaidi, Harith. "Secondary Metabolites from Xylaria Endophytes: The isolation and structure elucidation of secondary metabolites from Xylaria endophytes by chemical and spectroscopic methods." Thesis, University of Bradford, 2011. http://hdl.handle.net/10454/17479.
Повний текст джерелаAlhaidari, Rwaida A. A. "Secondary metabolites from Xylariaceous fungi. The isolation and structure elucidation of secondary metabolites from Xylariaceous fungi by chemical and spectroscopic methods." Thesis, University of Bradford, 2012. http://hdl.handle.net/10454/5705.
Повний текст джерелаMinistry of Higher Education; Kingdom of Saudi Arabia.
Alhaidari, Rwaida Adel. "Secondary metabolites from Xylariaceous fungi : the isolation and structure elucidation of secondary metabolites from Xylariaceous fungi by chemical and spectroscopic methods." Thesis, University of Bradford, 2012. http://hdl.handle.net/10454/5705.
Повний текст джерелаSchilling, Franz [Verfasser]. "Novel Methods for NMR - Single-SHOT Correlation Spectroscopy and Diffusion of 13C-Metabolites utilizing both Hyperpolarization and Optimal Control / Franz Schilling." München : Verlag Dr. Hut, 2013. http://d-nb.info/1130261336/34.
Повний текст джерелаSjögren, Jörgen. "Bioassay-guided isolation and characterisation of antifungal metabolites : studies of lactic acid bacteria and propionic acid bacteria /." Uppsala : Dept. of Chemistry, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/200517.pdf.
Повний текст джерелаLazariev, Andrii. "A quantum mechanics-based approach for optimization of metabolite basis-sets : application to quantitation of HRMAS-NMR signals." Phd thesis, Université Claude Bernard - Lyon I, 2011. http://tel.archives-ouvertes.fr/tel-00843311.
Повний текст джерелаSchilling, Franz [Verfasser], Steffen J. [Akademischer Betreuer] Glaser, Silvio [Akademischer Betreuer] Aime, and Axel [Akademischer Betreuer] Haase. "Novel Methods for NMR - Single-SHOT Correlation Spectroscopy and Diffusion of 13C-Metabolites utilizing both Hyperpolarization and Optimal Control / Franz Schilling. Gutachter: Silvio Aime ; Steffen J. Glaser ; Axel Haase. Betreuer: Steffen J. Glaser." München : Universitätsbibliothek der TU München, 2013. http://d-nb.info/1034420852/34.
Повний текст джерелаOjo, Catherine A. "Analysis & automatic classification of nuclear magnetic resonance signals." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4109.
Повний текст джерелаSalmon, Deborah Louise. "Metabolite profiling of the coccolithophore Emiliania huxleyi to examine links between calcification and central metabolism." Thesis, University of Exeter, 2013. http://hdl.handle.net/10871/14932.
Повний текст джерелаBucur, Adriana. "Spectométrie de RMN quantitative in vivo pour la mesure des lipides hépatiques chez l'homme et des métabolites cérébraux chez un modèle murin de neuro-inflammation." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10094.
Повний текст джерелаThe proton MRS is a unique non-invasive method to quantitative biochemical exploration of living tissues. The studies presented in this thesis aim to handle each one of the involved steps, from data acquisition to reliable and precise metabolic profile estimation of explored tissues. Protocols for experimental acquisition of in vivo, short echo-time magnetic resonance signals were defined, and optimized for a pre-clinical application (mice) on a 4.7T scanner and for a clinical study at 1.5T. The first study allowed yo measuring cerebral metabolite (N-acetyl-aspartate, choline, creatine, taurine) alterations along time in a murine model of neuro-inflammation on a 4.7T scanner and the second study aimed to measure the total quantity and the composition of liver fat in patients with hepatic steatosis in a clinical environment at 1.5T. Signal processing methods for metabolite and fat contribution estimates, coping with physical signal properties were validated for both studies. These methods are based on non-linear least squares algorithms. Multiple starting values and constraints strategies were successfully validated. The assets and the originality of this project are based on the synergic developments of acquisition protocols and the associated signal processing methods. These developments were designed to enrich the list of the biochemical information non-invasively measured, in order to help medical prognostic and diagnostic
Chen, Kan. "Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome." ScholarWorks@UNO, 2008. http://scholarworks.uno.edu/td/899.
Повний текст джерелаMARCHETTI, LUCIA. "Tecniche innovative per la caratterizzazione di prodotti naturali quali fonti di composti attivi e valutazione della loro attività biologica." Doctoral thesis, Università degli studi di Modena e Reggio Emilia, 2022. http://hdl.handle.net/11380/1278348.
Повний текст джерелаPlant extracts mainly consist in complex mixtures of chemically different bioactive compounds also known as secondary metabolites. In most cases, the bioactivity of natural products is not ascribable to a single compound, but rather to a multitude of them acting in a synergistic way. In this view, the complete definition of all the phytochemical constituents represents a key point to ensure the efficacy, reliability, and safety in the use of herbal medicines. Even today, the quality and authenticity assessment of herbal raw materials is frequently impaired by the lack of validated analytical methods and by the limited availability of certified references. Techniques for the analysis of herbal extracts and nutraceuticals need to be developed and upgraded continuously since different combinations of ingredients are employed in new formulations. In view of the above, the current PhD project was focused on the development and application of innovative analytical methods for the analysis of plant bioactive secondary metabolites. Particular attention was paid to plants of pharmaceutical (Cannabis sativa L., and Spathodea campanulata P. Beauv.) and nutraceutical interest (Morus alba L., Aloysia polystachya Griseb. et Moldenke). Different in-vitro assays for the bioactivity evaluation of these plants’ extracts were also the object of this PhD Thesis. In Chapter 1, the NMR technique was applied for the determination of the main non-psychoactive cannabinoids in fiber-type Cannabis female inflorescences. The analytical method was developed and fully validated to show compliance with international requirements. This technique finds application in the monitoring of the plant material, to guarantee a better reproducibility for biological assays, and to ensure the efficacy and safety of Cannabis-derived products. As a continuation of this project, the potentialities and drawbacks of separation and non-separation methods for the analysis of cannabinoids were considered and compared. Finally, C. sativa non-psychoactive varieties were selected for the preparation of cannabinoids rich extracts to evaluate their antiproliferative activity, by using cellular models. In Chapter 2 the chemical composition of Italian mulberry was investigated, as a source of phytochemicals in the context of new therapeutic approaches for diabetes mellitus. The first aim was to assess the quality of the plant material in comparison to Far Eastern Asia cultivations, where mulberry has been considered as a medicine for the treatment of diabetes for decades. The antiglycative capacity and hypoglycaemic effect of leaf extracts were evaluated on different in vitro models, to understand the potentialities of mulberry for the therapy of hyperglycaemia. Finally, the development of novel strategies for delaying the release of mulberry active constituents and enhancing therapeutic results will be discussed and are intended as the conclusion of this research project. In Chapter 3 the chemical composition of S. campanulata was investigated by HPLC-ESI-MS2, to clarify which are the most active components responsible for the inhibition of H. pylori growth. The aim was also to disclose the mechanisms underlying the biological activity. In Chapter 4 the phenolic composition of A. polystachya was investigated by means of three different MS techniques coupled to HPLC. The analytical method was developed and validated for the identification of metabolites and some phenylpropanoids were detected in this plant for the first time.
Martel, Dimitri. "Spectroscopie 2D de corrélation quantitative : Méthode de quantification, études expérimentales et applications in vivo." Thesis, Lyon, INSA, 2015. http://www.theses.fr/2015ISAL0004/document.
Повний текст джерелаIn in vivo Magnetic Resonance Spectroscopy (MRS), the main methods used allow metabolite concentration quantification using signals having one spectral dimension. This work focuses on the development of in vivo two dimensional correlated MRS in order to increase spectral resolution and quantification precision. The first axis is about the development of a method based on a 2D localized correlation MRS (L-COSY) for brain metabolite exploration. The L-COSY is implemented and studied on a small animal scanner. A dedicated quantification procedure operating in the acquisition domain is described. This latter is based on 1) a strong prior knowledge obtained by quantum mechanically simulate the effect of sequence on metabolite spin systems 2) a model function taking into account the relaxation weighting 3) constraints on the relaxation term linked to the field inhomogeneity effects which are assumed to act the same way on all the spins. Results are given experimentally using metabolites phantoms and through a comparison to other existing 2D MRS method, namely the J-resolved MRS (with the JPRESS sequence) using the Cramer Rao Lower Bounds (CRBs) theory. Although its inherent loss of signal to noise ratio is a disadvantage compared to J-PRESS, L-COSY quantification shows theoretically competitive relative CRBs, and even smaller CRBs for some coupled metabolites (e.g Glutamine or GABA), for an acquisition time similar to JPRESS. Second axis is about the adaptation of the 2D correlation MRS for the in vivo lipid metabolism study in the liver and subcutaneous adipose tissues of obese mice at 7T. This application shows the feasibility of 2D correlated MRS to be acquired on a moving organ and its quantitative relevance for triglyceride quantification and characterization in fatty liver and subcutaneous tissue
Dücker, Eibe Behrend. "Enhancement Strategies in NMR Spectroscopy." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E3E4-9.
Повний текст джерелаStonehouse, Jonathan. "New techniques in NMR spectroscopy." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360628.
Повний текст джерелаHughes, Colan Evan. "New techniques in NMR spectroscopy." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297524.
Повний текст джерелаFulwood, Russell. "Chiral analysis by NMR spectroscopy." Thesis, Durham University, 1992. http://etheses.dur.ac.uk/5979/.
Повний текст джерелаEdden, Richard Anthony Edward. "New methods in NMR spectroscopy." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613719.
Повний текст джерелаZhu, Jian-Ming. "Spatially localized proton NMR correlation spectroscopy." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq23681.pdf.
Повний текст джерелаNordstierna, Lars. "Molecular Association Studied by NMR Spectroscopy." Doctoral thesis, Stockholm : Physical Chemistry, Department of Chemistry, Royal Institute of Technology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3947.
Повний текст джерелаKenwright, A. "Applications of NMR spectroscopy to solids." Thesis, University of East Anglia, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.482971.
Повний текст джерелаHa, Dongwan. "Scalable NMR Spectroscopy with Semiconductor Chips." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11635.
Повний текст джерелаEngineering and Applied Sciences
Warne, Mark Anthony. "Theoretical studies in proton NMR spectroscopy." Thesis, University of Liverpool, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321165.
Повний текст джерелаSmith, Timothy Andrew David. "Conformational analysis studies in NMR spectroscopy." Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243220.
Повний текст джерелаStott, Katherine Mary. "Pulsed field gradients in NMR spectroscopy." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627246.
Повний текст джерелаAmero, Carlos D. "Protein Function Study by NMR Spectroscopy." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1205431343.
Повний текст джерелаFauré, De la Barra Nicole Eloísa. "Understanding immobilised enzymes by NMR spectroscopy." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7319/.
Повний текст джерелаKok, Trina. "Detection of brain metabolites in magnetic resonance spectroscopy." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/46602.
Повний текст джерелаIncludes bibliographical references (p. 59-61).
While magnetic resonance imaging (MRI) derives its signal from protons in water, additional and potentially important biochemical compounds are detectable in vivo within the proton spectrum. The detection and mapping of these much weaker signals is known as magnetic resonance spectroscopy or spectroscopic imaging. Among the complicating factors for this modality applied for human clinical imaging are limited chemical-shift dispersion and J-coupling that cause spectral overlap and complicated spectral shapes that limit detection and separation of several brain metabolites using MR spectroscopic imaging. Existing techniques for improved detection include so-called 2D spectroscopy, where additional encoding steps aid in the separation of compounds with overlapping chemical shift. This is achieved by collecting spectral data over a range of timing parameters and introducing an additional frequency axis, fl. Spectral editing techniques attempt to enhance metabolite signal detection along fl in order to resolve specific metabolite signals. While these techniques have been shown to improve signal separation, they carry a penalty in scan time and more complicated reconstruction compared to conventional spectroscopy, and are often prohibitive when combined with spectroscopic imaging. The task of this thesis is to characterize and optimize existing 2D spectroscopy techniques for improved metabolite resolution with reduced number of timing steps through numerical simulation and experimental validation in phantoms.
Trina Kok.
S.M.
Khajeh, Maryam. "Kinetic measurements using time-resolved NMR spectroscopy." Thesis, University of Manchester, 2010. https://www.research.manchester.ac.uk/portal/en/theses/kinetic-measurements-using-timeresolved-nmr-spectroscopy(aae85bb3-de19-450a-96ab-50e2dfd89da7).html.
Повний текст джерелаSäwén, Elin. "NMR spectroscopy and MD simulations of carbohydrates." Doctoral thesis, Stockholms universitet, Institutionen för organisk kemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-61569.
Повний текст джерелаBergin, Paul Gerald. "Applications of tritium and tritium NMR spectroscopy." Thesis, University of Surrey, 2002. http://epubs.surrey.ac.uk/842709/.
Повний текст джерелаHu, Song 1969. "Thrombin exosite interactions studied by NMR spectroscopy." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23897.
Повний текст джерелаVarma, S. C. "Polymer end-group studies using NMR spectroscopy." Thesis, Lancaster University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374665.
Повний текст джерелаZagdoun, Alexandre. "Dynamic Nuclear Polarisation Surface Enhanced NMR Spectroscopy." Phd thesis, Ecole normale supérieure de lyon - ENS LYON, 2014. http://tel.archives-ouvertes.fr/tel-01065554.
Повний текст джерелаEyles, Stephen J. "Studies of protein folding by NMR spectroscopy." Thesis, University of Oxford, 1995. http://ora.ox.ac.uk/objects/uuid:06b8fb16-790c-4b72-80d0-8317920655fe.
Повний текст джерелаGrieves, R. A. "Multinuclear NMR spectroscopy of transition metal complexes." Thesis, University of Bradford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372167.
Повний текст джерелаEdgar, Mark. "Studies in proton and flourine NMR spectroscopy." Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240501.
Повний текст джерелаCantalapiedra, Nuria Aboitiz. "Intramolecular hydrogen-bonding studies by NMR spectroscopy." Thesis, University of Liverpool, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366715.
Повний текст джерелаKraft, Robert A. (Robert Arthur) 1970. "In vivo two-dimensional NMR correlation spectroscopy." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/85271.
Повний текст джерелаLe, Guennec Adrien. "Fast 2D NMR spectroscopy for complex mixtures." Palaiseau, Ecole polytechnique, 2015. https://theses.hal.science/tel-01191697/document.
Повний текст джерелаMetabolomics is a recent area of research, which aims at analyzing the entirety of molecules involved in chemical reactions in an organism. Nuclear Magnetic Resonance (NMR) is widely used in metabolomics nowadays thanks to a methodology based on one-dimensional (1D) NMR. However, 1D spectra of complex mixtures, like biological extracts or fluids, are characterized by important overlap between peaks, which can be detrimental to the identification and quantification of molecules of interest. Two-dimensional (2D) NMR can be used to reduce the risk of overlap. However, the duration of 2D experiments is often prohibitive for metabolomics studies. Several approaches exist to reduce the duration of 2D experiments, but they have not been evaluated so far for metabolomics. In this thesis, we have shown the usefulness of fast 2D NMR for metabolomics and optimized its performances. Two fast 2D NMR approaches have been evaluated, ultrafast 2D NMR and non-uniform sampling. In a study with synthetic samples, we first demonstrated the usefulness of 2D NMR compared to 1D NMR, then we showed that the experimental time of 2D spectra could be reduced with fast 2D NMR approaches without loss of information. Then we optimized the use of fast 2D NMR for complex mixture analysis. First, we developed a new pulse sequence with ultrafast 2D NMR of proton, in order to suppress the diagonal peaks, which can overlap with correlation peaks and therefore reduce the information content of 2D spectra. Then non-uniform sampling was used to increase up to 32 times the resolution in the indirect dimension without increasing the experimental time and without loss of sensitivity or repeatability. Finally, we worked on automating the determination of relaxation constants in complex mixture. These tools open promising perspectives for high-throughput metabolomics of complex biological samples
Gao, Chun. "19F DOSY Diffusion NMR Spectroscopy of Fluoropolymers." University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1447069266.
Повний текст джерелаRogerson, Alexandria. "New techniques in diffusion-ordered NMR spectroscopy." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/new-techniques-in-diffusionordered-nmr-spectroscopy(aa3eaee0-984b-4434-b460-8c3118a7c3b2).html.
Повний текст джерелаInglis, Benjamin Alastair. "In vivo NMR spectroscopy of the brain." Thesis, Queen Mary, University of London, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.644797.
Повний текст джерелаMiletti, Teresa. "Enzyme flexibility studied by solution NMR spectroscopy." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119414.
Повний текст джерелаLe lien important entre la dynamique, la structure et la fonction des enzymes n'est pas encore bien défini et est donc une relation intéressante à étudier. Dans cette thèse, les enzymes NADH oxydase (NOX) de Thermus thermophilus et mycobacterium tuberculosis protéine tyrosine phosphatase B (PtpB) sont les systèmes choisis pour étudier l'influence des conditions de solution sur la structure et la flexibilité des protéines par rapport à leur activité catalytique. Les techniques de résonance magnétique nucléaire (RMN) et de calorimétrie de titration isothermique (ITC) ont été utilisées dans l'étude de NOX. La RMN et la fluorimétrie différentielle à balayage (DSF) ont été utilisées pour évaluer la stabilité de l'échantillon de PtpB dans diverses conditions de tampons. NOX est une enzyme homodimérique avec de nombreuses applications biotechnologiques potentielles. NOX accepte les cofacteurs flavine mononucléotide (FMN) ou flavine adénine dinucléotide (FAD). L'enzyme a une stabilité thermique élevée et son activité augmente avec l'addition de faibles concentrations d'urée. Utiliser des enzymes in vitro donne beaucoup de liberté pour contrôler leur environnement, utile pour examiner les changements qui se produisent dans différentes conditions. Ainsi, RMN nous a permis d'étudier ces caractéristiques intéressantes sur la dynamique et la structure de NOX. Lorsque Mtb infecte les cellules, il secrète PtpB qui perturbe les voies de signalisation et affaibli les réponses immunitaires. PtpB contient un couvercle de deux hélices qui enterre complètement le site actif et il est proposé qu'il ait évolué ainsi en protection contre les défenses chimiques et permet un accès au substrat. Une étude de la dynamique du domaine du couvercle de PtpB utilisant la RMN éluciderait la relation entre la conformation et la fonction de PTPB et contribuerait au développement d'inhibiteurs en tant que médicaments potentiels. Pour ceci, il est essentiel d'obtenir un échantillon stable qui donnera des spectres RMN de qualité optimale. RMN et DSF ont été utilisés pour optimiser les conditions d'échantillonnage de PtpB pour la RMN et la qualité spectrale. Nous avons utilisé une suite de nouvelles expériences de relaxation de 15N par RMN pour estimer l'ampleur de l'élargissement des signaux en raison de mouvements à l'échelle de temps des microsecondes et de mesurer les taux de relaxation transversale sans échange pour chaque résidu de NOX. Ces mesures nous ont permis d'identifier plusieurs résidus impliqués dans les processus dynamiques. De plus, les paramètres d'ordre, S2, déterminées à partir des expériences standards de T1, T2 et NOE, ont permis d'identifier des résidus additionnels caractérisés par des mouvements sur des échelles de temps de nanosecondes à picosecondes. Nous avons ensuite étudié les effets de la température, urée, la liaison de cofacteur et du produit à NOX en utilisant la RMN et le ITC. Pour chaque condition étudiée, la variation de déplacement chimique des signaux RMN n'est significative par rapport à tous les autres signaux que pour un petit sous-ensemble récurrent de résidus. Cet amas de résidus est situé autour du site actif de l'enzyme et ainsi, suggère preuve d'une relation entre un réarrangement conformationnel concertée de la structure de NOX et de son activité catalytique. Une augmentation de la qualité spectrale de RMN et un échantillon de PtpB pour la RMN avec une meilleure stabilité thermique, des rendements plus élevés et une agrégation réduite ont été obtenus pour de futures expériences dynamiques par RMN. Ceci a été réalisé en optimisant les conditions de l'échantillon en effectuant plusieurs titrages par RMN et des expériences de DSF. L'évaluation de la stabilité thermique des enzymes avec différents additifs à l'aide de DSF pourrait facilement être introduit lors de l'élaboration de protocoles de purification de protéines comme une méthode routinière pour optimiser les conditions d'échantillons pour les études par RMN.