Дисертації з теми "Next Generation Rating Approach"
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Gatri, Aymen [Verfasser]. "Next Generation Optical Wireless Communication Systems : A Systems Approach / Aymen Gatri." Hamburg : disserta Verlag, 2019. http://d-nb.info/1199410845/34.
Повний текст джерелаSeung, Taehun. "Holistic-Lightweight Approach for actuation systems of the next generation aircraft." Universitätsverlag Chemnitz, 2017. https://monarch.qucosa.de/id/qucosa%3A34400.
Повний текст джерелаGegenwärtig konzentriert sich die Technologieentwicklung für Flugzeuge auf die Reduktion des Energieverbrauchs mehr denn je zuvor. Hierfür ist die Effizienz der an Bord befindlichen, nicht propulsiven Subsysteme neben der Wirkungsgradverbesserung der Triebwerke von zentraler Bedeutung. Laut vorangegangenen Untersuchungen und Studien ist die Vereinfachung bzw. Vereinheitlichung der Vielfalt der konventionellen Bordenergiesysteme durch ein adäquates Energiemanagement unter Verwendung von Elektrizität der aussichtsreichte Weg zur Effizienzverbesserung auf der Gesamtflugzeugebene. Durch die Elektrifizierung wurden die einzelnen Geräte zwar zuverlässiger und energieeffizienter als je zuvor aber gleichzeitig erheblich schwerer, sodaß ein signifikanter Verlust an Nutzlasten auf Gesamtflugzeugebene hervorgerufen wird. Das Hauptziel der vorliegenden Arbeit war es, ein Schwerlast-EHA-System mit mehrfachen Betätigungseinheiten durch Einführung von umfassenden Perspektiven zu optimieren. Durch Einführung der sog. ganzheitlichen Leichtbauweise demonstriert die Arbeit, wie das Subsystem mit mehreren Endgeräten ultimativ optimiert werden kann, ohne Abstriche an Gewichtsbilanz u/o Kompromiß mit der Energieeffizienz zu machen. Um eine wahrhaftige Optimierung, d.h. die Erreichung des ultimativen, Nonplusultra-Verbesserungslevels zu erreichen, wurden die Systemarchitektur, die Hardware und die Operationsmethode interaktiv kombiniert, wobei die besondere Aufmerksamkeit auf die interaktiven, zur Verbesserung führenden Einflüsse gelegt wurde. Die Minimierung des Energieverbrauchs und die ultimative Gewichtsoptimierung gleichzeitig können erreicht werden, wenn die physikalischen Zusammenhänge zwischen den involvierten Subsystemen verstanden und ihre verborgenen Potentiale ausgenutzt werden. Der einzige und vernünftige Weg zur Erreichung der ultimativen Optimierung eines Betätigungssystems ist eine allumfassende Betrachtung, also eine ganzheitliche Betrachtungs- bzw. Vorgehensweise.
Rybo, Frida, and Alfred Söder. "Next generation expedition stove : A lead user approach to product development." Thesis, KTH, Maskinkonstruktion (Avd.), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-277902.
Повний текст джерелаÅr 1996 lanserade Primus sitt expeditionskök Multifuel, vilket var det första köket som kunde användas med gasol, kemiskt ren bensin, fotogen och flygbränsle. Multifuel och ytterligare två flerbränslekök är idag en del av företagets expeditionssegment, som designades för att prestera under extrema förhållanden. Expeditionsköken har inte förändrats avsevärt sedan de togs fram, vilket ledde till att Primus ville undersöka hur framtiden för dessa kök ser ut. Eftersom expeditionsköken symboliserar företagets arv är det ett viktigt segment för dem. Primus satte upp ett internt mål att uppdatera expeditionssegmentet inom de närmsta åren, vilket var huvudorsaken till att detta examensarbete utfördes. Syftet med projektet var att undersöka användarvänligheten hos expeditionsköken och förstå användarnas behov för att på så sätt besluta om en riktning för nästa generations expeditionskök. Målet med arbetet var att leverera både riktlinjer för kommande produktutveckling och ett välmotiverat produktkoncept som är baserat på de framtagna riktlinjerna. Under arbetets gång har en anpassad variant av lead user-metoden använts, vilket betyder att ett antal utvalda användare med värdefulla egenskaper har involverats genom arbetsprocessen. Omfattande undersökningar har gjorts genom bland annat intervjuer och användarstudier. Den kvalitativa datan analyserades med inspiration av Gioia-metodologin och resulterade i en överlämning med riktlinjer för produktutveckling. Detta innefattar insikter från analysen, en användarcentrerad omsegmentering av Primus expeditionssegment samt en identifiering av luckor på marknaden. Det blev tydligt att ett expeditionsgaskök borde inkluderas i segmentet eftersom professionella användare visade stor vilja att använda gas och behovet för flerbränslekök var litet. Baserat på riktlinjerna togs ett produktkoncept fram. Primus Altitude Ti är ett expeditionsgaskök mestadels bestående av titan med en bred brännare som använder sig av tekniken för ett laminärt flöde, vilket ger en tyst och bränsleeffektiv användning. Köket har en steglös ventil vid brännaren samt en ventil med två lägen, på och av, vid gasbehållaren som tillåter drift av köket med gasbehållaren uppochned vilket är fördelaktigt vid kalla förhållanden.
Hibbert, Kirk R. "A need for systems architecture approach for next generation mine warfare capability." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2006. http://library.nps.navy.mil/uhtbin/hyperion/06Sep%5FHibbert.pdf.
Повний текст джерелаThesis Advisor(s): John Osmundson, Martha Jallim Hall. "September 2006." Includes bibliographical references (p. 79-83). Also available in print.
Heslop, Janelle Nicole. "A systematic approach for assessing next generation technologies and solutions in biomanufacturing." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122586.
Повний текст джерелаThesis: S.M., Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, 2019, In conjunction with the Leaders for Global Operations Program at MIT
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 92-93).
Amgen is one of the world's leading independent biotechnology companies and competes globally to advance important medicines in a highly competitive marketplace. Biologics manufacturers such as Amgen have traditionally invested in costly, large-scale stainless steel infrastructure to support the production of biologic medication. However, more recently, changes in the economics, such as the need to deploy less-capital intensive biomanufacturing plants faster, and advances in the technology, such as process-intensification (i.e., getting more protein from each cell), have created both incentives and pressures for smaller-scale, single-use, and modular production technologies. These incentives include greater flexibility, shorter timelines for construction / rapid deployment of new facilities, and reduced costs as well as physical and environmental footprint.
To prepare for this changing business environment, Amgen must develop a manufacturing strategy that can enable the production of high quality products with significant reduction in timelines, cost, and reduced impact. To do so, Amgen is investigating a handful of these new production technologies, known as next generation manufacturing technologies, and attempting to understand their applicability in their future manufacturing model. There is a need for a transparent and standard methodology for evaluating and deploying new technologies in the manufacturing network. This study aims to address this issue and enable speed, rigor, and efficiency of decisionmaking through the use of a structured framework for selection and deployment of next generation technologies. Through literature review and engagement with Amgen experts, this study defines a next generation manufacturing technology evaluation framework.
This framework involves a hybrid, multi-attribute set of metrics that are broadly categorized into economic, environmental, and operational assessment areas. The framework is then applied to assess the economic, operational, and environmental implications of deploying single use technologies in drug substance manufacturing as a test of concept. An assessment along the three areas helps to identify that single use technologies, namely single use bags due to their cost and environmental footprint, may not always be the optimum substitute for all existing process technology. Instead, a hybrid approach, mixing new single use technology with existing stainless steel infrastructure, may help to reduce variable cost and carbon footprint of the process.
When the framework and this proposed hybrid approach was at an Amgen site, a potential savings of up to $ 1 M per year was identified as well as the elimination of up to thousands of liters in clean water losses, and up to 400x reduction in the carbon footprint of the process. Lastly, the assessment framework is applied as a management tool in the assessment of next generation drug product filling technology to demonstrate how the framework can be used to enable rapid decision-making related to future manufacturing scenarios.
by Janelle Nicole Heslop.
M.B.A.
S.M.
M.B.A. Massachusetts Institute of Technology, Sloan School of Management
S.M. Massachusetts Institute of Technology, Department of Civil and Environmental Engineering
Okwo, Adaora. "Next-generation biofuels: the supply chain approach to estimating potential land-use change." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/47603.
Повний текст джерелаNguyen, Thien. "Coordinated behavior for predator prey modeling an approach to next generation console development /." [Gainesville, Fla.] : University of Florida, 2008. http://purl.fcla.edu/fcla/etd/UFE0022893.
Повний текст джерелаSung, Che-ming, and 宋志明. "A network approach to the study of the next generation in the mobile telephone market." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1995. http://hub.hku.hk/bib/B31266824.
Повний текст джерелаSung, Che-ming. "A network approach to the study of the next generation in the mobile telephone market /." Hong Kong : University of Hong Kong, 1995. http://sunzi.lib.hku.hk/hkuto/record.jsp?B1470898X.
Повний текст джерелаSilva, Hector E. "Enterprise design for services : a systems approach for the Boeing next generation corporate travel system architecture." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/59171.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (p. 72).
Typically a company's second largest controllable expense, corporate travel affects many employees at Boeing. A challenge when implementing improvements in the travel and expense system, which is actually comprised of a system of systems, is the coordination of various solutions to ensure improvements in one area do not adversely affect the efficiency of other areas. Various systems along with the people, policies, and processes used to provide services to travelers must be coordinated both inside and outside Boeing in order for overall travel operations to function properly. The intent of this project is to establish a systems-based architecture for Boeing's Next Generation Travel System. This thesis proposes re-designing the Boeing travel system using an enterprise architecting framework to select a future state architecture for a service organization. The analysis recommends a supplier integrated "off-the-shelf' software solution, employing the software as a service business model. Under this model the supplier is paid per transaction completed in the system; adoption of this metric aligns the system to reduce re-work costs, increase first-time pass quality, and improve usability. Additionally, new supplier software tools will allow the Boeing travel organization to transition from a process-focused to a more knowledge-focused service team.
by Hector E. Silva.
S.M.
M.B.A.
Moreno, Cabrera José Marcos. "A translational bioinformatics approach to improve genetic diagnostics of hereditary cancer using next-generation sequencing data." Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/672364.
Повний текст джерелаYu, Zehao. "Towards location-awareness in next generation wireless networks : a new approach based on channel state information." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/127117.
Повний текст джерелаCataloged from the official PDF of thesis.
Includes bibliographical references (pages 69-78).
Location-awareness in next generation wireless networks will be a key enabler for numerous emerging applications. Recently, a novel approach to localization based on soft information (SI), exploiting all positional information inherent in measurement and contextual data, has been proposed. This thesis further develops SI-based localization by establishing a new approach relying on channel state information (CSI) measurements. In particular, we design an efficient joint message-passing (MP) localization algorithm, which consists of two layers: the transformation layer and the estimation layer. The transformation layer extracts SI of the channel impulse response (CIR) from CSI measurements using a sparsity promoting prior model, which addresses the difficulty of unknown number of multipath in estimating the CIR. The estimation layer infers node positions based on the SI of the CIR using a delay-origin uncertainty model, which describes the conditional distribution of the delays in the CIR given node positions. Simulation results using QuaDriGa channel simulator show that our localization algorithm achieves decimeter-level localization accuracy for both Wi-Fi and mmWave signals, which outperforms conventional algorithms.
by Zehao Yu.
S.M.
S.M. Massachusetts Institute of Technology, Department of Aeronautics and Astronautics
Ying, Daidong. "Energy-Efficient and Secure Device-to-Device Communications in the Next-Generation Wireless Network." University of Dayton / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1532458376184496.
Повний текст джерелаCandelli, Tito. "NOVEL APPROACH TO STORAGE AND STORTING OF NEXT GENERATION SEQUENCING DATA FOR THE PURPOSE OF FUNCTIONAL ANNOTATION TRANSFER." Thesis, Högskolan i Skövde, Institutionen för vård och natur, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-6043.
Повний текст джерелаKwok, Wing Hei Harold. "New Approach in Fabrication of Solid-State Nanopore for Bio-Sensing Applications." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32210.
Повний текст джерелаAlvarez, Rodriguez Alberto. "An integrated framework for the next generation of Risk-Informed Performance-Based Design approach used in Fire Safety Engineering." Digital WPI, 2013. https://digitalcommons.wpi.edu/etd-dissertations/5.
Повний текст джерелаSomavarapu, Sashi K. (Sashi Kanth). "System dynamics approach to understand the role of information technology in the evolution of next generation integrated product development systems." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/33419.
Повний текст джерелаIncludes bibliographical references (p. 96-97).
For the automotive industry, to be competitive in the market place has to devise many strategies. Some of the prominent strategies include and are not limited to, reduction of development costs by moving in-house work to its suppliers, reduction of PD cycle time by mimicking some of the industry's successful PD processes. Some companies are also devising some complex strategies like Zero-Prototype development using computer aided prototyping and testing, currently prevalent in the aerospace and naval industries, and more recently making a move into the Lean PD systems and processes to avoid waste and increase efficiency. However, to introduce such lean PD systems, with reduced PD cycle time, into a complex organization with many internally developed IT systems, processes and tools is a huge challenge. The organization needs to adapt to these lean environments not just structurally but also culturally. To design a lean PD organization (system) the decision makers have to foresee and understand how the system of systems may react to the change before they are implemented and/or executed. In the past couple of decades IT systems have been a primary enabler for PD work flow processes.
(cont.) However, IT systems are so engraved in some PD organizations that they have turned into an engineering process mechanism. Also, some of the IT systems have served more than their life expectancy and in some cases cannot be decommissioned because these systems are so tightly coupled with the business processes. An understanding of the internal system dynamics of these deeply engraved IT systems in the PD life cycle will help the automotive industry executives (decision makers) and IT systems architects to make the right decision when designing and deploying the new PD systems or processes. This study provides an overview of how IT tools have evolved in the automotive industry. Extensive research was conducted to understand the different system dynamics tools used in industry - specifically in automotive product development and the software development areas. The study concludes with an explanation of how system dynamics tools can be used as a program planning and management tool.
by Sashi K. Somavarapu.
S.M.
Coetzee, Beatrix. "A metagenomic approach using next-generation sequencing for viral profiling of a vineyard and genetic characterization of grapevine virus E." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5186.
Повний текст джерелаIncludes bibliography.
Title page: Dept. of Genetics, Faculty of Science
ENGLISH ABSTRACT: Next-generation sequencing technologies are increasingly used in metagenomic studies, largely due to the high sequence data throughput capacity and unbiased approach in determining the genetic composition of an unknown environmental sample. This study investigated the applicability of the Illumina next-generation sequencing platform for metagenomic sequencing of grapevine viruses to provide the first complete viral profile, or virome, of a diseased vineyard. Leaf material was harvested from 44 randomly selected vines in a leafroll-diseased vineyard in South Africa. Sample material was pooled and double-stranded RNA extracted. The dsRNA was sequenced as a paired-end sequencing run using the Illumina sequencing-by-synthesis technique, and more than 19 million sequence reads, equivalent to approximately 837 megabases of metagenomic sequence data, were obtained. Of these data, approximately 400 megabases could be assembled into 449 scaffolds, using the de novo assembler Velvet. These scaffolds were subjected to BLAST searches against the NCBI databases and top hit scores were used for virus identification. Based on the BLAST results, suitable sequences were selected from the NCBI database and used as reference sequence in MAQ mapping assemblies. The bioinformatic analyses allowed for the determination of the virus species present, the most prominent variants, and the relative abundance of each. Four known grapevine viral pathogens were identified. Grapevine leafroll-associated virus 3, representing 59% of the analyzed short read sequence data, was identified as the most prominent virus species. Three variants of this virus were detected: GP18 was the most abundant, followed by a minor Cl766/NY1 variant and a potential novel grapevine leafroll-associated ampelovirus. A single Grapevine rupestris stem pitting ]associated virus variant, similar to SG1, and a Grapevine virus A variant, a member of molecular group III, were identified. This study is also the first to report the presence of Grapevine virus E (GVE) in South African vineyards. Grapevine virus E was further genetically characterized and the genome sequence of GVE isolate SA94 determined. The GVE SA94 genome sequence, 7568 nucleotides in length, is the first complete genome sequence for the virus species. The genome organization of GVE SA94 is typical of vitiviruses, but in contrast to other RNA viruses, the AlkB domain is located within the helicase domain in open reading frame 1 (ORF 1). Grapevine virus E SA94 shares nearly 100% nucleotide identity with the Japanese TvP15 isolate and GVE 3404, a de novo scaffold generated from the metagenomic sequence data. Bioinformatic analysis of metagenomic sequence data further revealed the presence of three fungus-infecting viral families, Chrysoviridae, Totiviridae and the unclassified dsRNA virus, Fusarium graminearum dsRNA mycovirus 4. A virus from the family Chrysoviridae, similar to Penicillium chrysogenum virus, was the second most abundant virus detected. We demonstrated the successful application of a short read sequencing technology, such as the Illumina platform, for viral profiling of an infected vineyard. To our knowledge this is the first application of the Illumina technology for this purpose.
AFRIKAANSE OPSOMMING: Volgende-generasie tegnologie om basis volgordes van nukleiensure te bepaal, word al meer gebruik in metagenomiese studies. Dit is veral weens die hoe data-omset kapasiteit en onbevooroordeelde aanslag in die bepaling van die genetiese samestelling van onbekende omgewingsmonsters. Hierdie studie het die aanwending van die Illumina volgende-generasie volgorde-bepalingsplatform in 'n metagenomiese studie van wingerdvirusse, ondersoek. Dit het ten doel gehad om die eerste volledige virus profiel, of viroom, van 'n geinfekteerde wingerd saam te stel. Blaarmateriaal is verkry vanaf 44 lukraak-gekose wingerdstokke in 'n rolblad-geinfekteerde wingerd in Suid-Afrika. Monster materiaal is saamgevoeg en dubbelstring-RNS geekstraheer. Die dubbelstring-RNS is onderwerp aan gepaarde-ent volgorde-bepaling deur gebruik te maak van die Illumina volgorde-bepaling-deur-sintese tegniek. Meer as 19 miljoen volgorde reekse, ekwivalent aan ongeveer 837 megabasisse volgorde data, is verkry. Van hierdie data kon ongeveer 400 megabasisse saamgevoeg word in 449 konstrukte ("scaffolds"), deur gebruik te maak van die de novo samesteller Velvet. Hierdie konstrukte is onderwerp aan BLAST soektogte teen die NCBI databasisse en die hoogste trefslag-telling is gebruik vir virus identifikasie. Op grond van die "BLAST" resultate is geskikte volgordes geselekteer vanaf die NCBI databasis en gebruik as verwysingvolgordes in MAQ kartering-analises. Met die bioinfomatika analises kon die virus spesies teenwoordig, asook die mees prominente variante en relatiewe voorkoms van elk, bepaal word. Vier bekende virus wingerdpatogene is geidentifiseer. Grapevine leafroll-associated virus 3, verteenwoordig deur 59% van die geanaliseerde kort-reeks volgorde data, is identifiseer as die mees prominente virus spesie. Drie variante van die virus is in die wingerdmonster opgespoor: GP18 kom die mees algemeen voor, gevolg deur 'n CL-766/NY1 variant en 'n potensiele nuwe wingerd rolblad-geassosieerde ampelovirus. 'n Enkele Grapevine rupestris stem pitting-associated virus variant, soortgelyk aan SG1, en 'n Grapevine virus A variant, 'n lid van molekulere groep III, is geidentifiseer. Hierdie studie is ook die eerste om die teenwoordigheid van Grapevine virus E (GVE) in Suid-Afrikaanse wingerde te rapporteer. Grapevine virus E is verder geneties gekarakteriseer en die genoomvolgorde van GVE isolaat SA94 is bepaal. Die GVE SA94 genoomvolgorde, 7568 nukleotiede lank, is die eerste volledige genoomvolgorde vir hierdie virus spesie. Die genoomorganisasie is tipies van vitivirusse, maar in kontras met ander RNA virusse is die AlkB domein binne-in die helikase domein van oopleesraam 1 (ORF 1) geleë. Grapevine virus E SA94 deel byna 100% nukleotied identiteit met die Japannese TvP15 isolaat en GVE 3404, 'n de novo konstruk gegenereer vanaf die metagenomiese volgorde data. Bioinformatika analises van die metagenomiese volgorde data het verder die teenwoordigheid van drie swam-infekterende virus families, die Chrysoviridae, Totiviridae en ongeklassifiseerde dubbelstring-RNS virus, Fusarium graminearum dsRNA mycovirus 4, aangetoon. 'n Virus van die Chrysoviridae familie, soortgelyk aan Penicillium chrysogenum virus, het die tweede meeste voorgekom in die wingerd monster. Hierdie studie demonstreer die suksesvolle toepassing van 'n kort reeks volgorde-bepalingstegnologie soos die Illumina platform, vir die opstel van 'n virusprofiel van 'n geinfekteerde wingerd. Sover ons kennis strek is hierdie die eerste aanwending van die Illumina tegnologie vir hierdie doel.
Labuschagne, Jan Phillipus Lourens. "Development of a data processing toolkit for the analysis of next-generation sequencing data generated using the primer ID approach." University of the Western Cape, 2018. http://hdl.handle.net/11394/6736.
Повний текст джерелаSequencing an HIV quasispecies with next generation sequencing technologies yields a dataset with significant amplification bias and errors resulting from both the PCR and sequencing steps. Both the amplification bias and sequencing error can be reduced by labelling each cDNA (generated during the reverse transcription of the viral RNA to DNA prior to PCR) with a random sequence tag called a Primer ID (PID). Processing PID data requires additional computational steps, presenting a barrier to the uptake of this method. MotifBinner is an R package designed to handle PID data with a focus on resolving potential problems in the dataset. MotifBinner groups sequences into bins by their PID tags, identifies and removes false unique bins, produced from sequencing errors in the PID tags, as well as removing outlier sequences from within a bin. MotifBinner produces a consensus sequence for each bin, as well as a detailed report for the dataset, detailing the number of sequences per bin, the number of outlying sequences per bin, rates of chimerism, the number of degenerate letters in the final consensus sequences and the most divergent consensus sequences (potential contaminants). We characterized the ability of the PID approach to reduce the effect of sequencing error, to detect minority variants in viral quasispecies and to reduce the rates of PCR induced recombination. We produced reference samples with known variants at known frequencies to study the effectiveness of increasing PCR elongation time, decreasing the number of PCR cycles, and sample partitioning, by means of dPCR (droplet PCR), on PCR induced recombination. After sequencing these artificial samples with the PID approach, each consensus sequence was compared to the known variants. There are complex relationships between the sample preparation protocol and the characteristics of the resulting dataset. We produce a set of recommendations that can be used to inform sample preparation that is the most useful the particular study. The AMP trial infuses HIV-negative patients with the VRC01 antibody and monitors for HIV infections. Accurately timing the infection event and reconstructing the founder viruses of these infections are critical for relating infection risk to antibody titer and homology between the founder virus and antibody binding sites. Dr. Paul Edlefsen at the Fred Hutch Cancer Research Institute developed a pipeline that performs infection timing and founder reconstruction. Here, we document a portion of the pipeline, produce detailed tests for that portion of the pipeline and investigate the robustness of some of the tools used in the pipeline to violations of their assumptions.
Mori, Minako. "Pathogenic mutations identified by a multimodality approach in 117 Japanese Fanconi anemia patients." Kyoto University, 2019. http://hdl.handle.net/2433/243302.
Повний текст джерелаSeung, Taehun [Verfasser], Lothar [Akademischer Betreuer] Kroll, Lothar [Gutachter] Kroll, and Piotr [Gutachter] Gendarz. "Holistic-Lightweight Approach for actuation systems of the next generation aircraft / Taehun Seung ; Gutachter: Lothar Kroll, Piotr Gendarz ; Betreuer: Lothar Kroll." Chemnitz : Universitätsverlag Chemnitz, 2019. http://d-nb.info/1219581917/34.
Повний текст джерелаBasu, Piyali. "A novel whole system integrated genomics approach to identify key genetic components which facilitate synthetic design of a genetically engineered strain of Escherichia coli K12 with enhanced isobutanol tolerance." Thesis, Brunel University, 2016. http://bura.brunel.ac.uk/handle/2438/13712.
Повний текст джерелаNguyen, Viet Tuan. "An evaluation of potential candidate genes involved in salinity tolerance in striped catfish (Pangasianodon hypopthalmus) using an RNA-SEQ approach." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/84924/4/Viet_Tuan_Nguyen_Thesis.pdf.
Повний текст джерелаRobertson, Laura, Andrea Lowery, Lindsay Lester, and Renee Rice Moran. "The Intersection of 5Es Instruction, and the Claims, Evidence, and Reasoning Framework: A Hands-on Approach Supporting the NGSS in Upper Elementary Classrooms." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/1308.
Повний текст джерелаPinney, Brian Robert John. "Characterizing the changes in teaching practice during first semester implementation of an argument-based inquiry approach in a middle school science classroom." Diss., University of Iowa, 2013. https://ir.uiowa.edu/etd/4721.
Повний текст джерелаZegarra, León Zegarra León. "A combinatorial approach to query the PknG interactome of Mycobacterium tuberculosis." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2019. http://hdl.handle.net/10757/628099.
Повний текст джерелаThe ability of Mycobacterium tuberculosis to survive inside the macrophage greatly contributes to its pathogenicity, latency and persistence during infection. This bacillus induces alterations in the intraphagosomal environment and inhibits phagosome maturation, thus promoting mycobacterial survival. M. tuberculosis PknG hijacks the macrophage precisely by avoiding phagosome-lysosome fusion. In this sense, PknG represents a family of novel targets to cope with the need for new antimicrobials for latent tuberculosis. Here, we aimed to: (i) elucidate the structural-molecular basis of ATP and Mg2+ as PknG cofactors; (ii) characterize the kinetic parameters governing PknG:ATP complex formation; and, (iii) identify PknG-binding peptides to experimentally query PknG’s interactome using combinatorial approach such as Phage Display. Our results confirm that PknG exclusively binds to ATP with a dissociation constant (KD) of 108.8 22.9 µM. Mg2+ thermally stabilizes PknG in an ATP-dependent manner. Pre-steady-state analyses show that ATP binding and dissociation are rapid in the PknG:ATP complex. Using PknGN-Ext, TPR we solved the ADP-state crystal structure while showing that ATP precludes crystallization. Phage Display and bioinformatic analyses identified 57 potential PknG binders. A close comparison to the M. tuberculosis proteome provided a subset of 20 proteins that may interact with PknG. Our results confirmed five previously reported PknG-associated proteins: PknG, DnaK chaperone, ABC transporter Rv1747, Ribosomal Protein L23 and Elongation Factor Tu, highlighting our platform’s validity to uncover the PknG interactome. Altogether, our results reveal putative protein-protein interactions that may play a role in mycobacterial survival, while also providing solid bases for the development of anti-tuberculosis drugs by disrupting these interactions or exploiting these lead-like peptide molecules.
Tesis
Robertson, Laura, Harold Kelley, Scott Honeycutt, and Kari Eubanks. "From Molecules to Organisms (LS1): An Integrated, Hands-on Approach Supporting NGSS and CCSS ELA." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/1309.
Повний текст джерелаRobertson, Laura, Renee Rice Moran, Chih-Che Tai, LaShay Jennings, Huili Hong, and Diana O'Neal. "Force and Motion: An Integrated K-8 Hands-On Approach Supporting the NGSS and CCSS ELA." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/1312.
Повний текст джерелаRobertson, Laura, LaShay Jennings, Kari Eubanks, and Scott Honeycutt. "Photosynthesis and Cellular Respiration (LS1): A Hands-On Approach Supporting the NGSS and ELA CCSS." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/1313.
Повний текст джерелаKruse, Colin Peter Singer. "Data-Enabled Approach to Characterize Dynamic Regulatory Pathways in Two Kingdoms." Ohio University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1573746719306039.
Повний текст джерелаLacoste, Deixonne Caroline. "Apport du séquençage haut débit dans l'amélioration de la prise en charge des maladies monogéniques." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM5062/document.
Повний текст джерелаThe diffusion of Next Generation Sequencing (NGS) technologies induces an important change that modifies molecular diagnostics indications and prompts laboratories to re-think their diagnostic strategies, up-to-now based on Sanger sequencing routine. Several high throughput approaches are available from the sequencing of a gene panel, to a whole exome, or even a whole genome. In all cases, a tremendous amount of data are generated, that have to be filtered, interpreted and analyzed by the use of powerful bioinformatics tools.In part 1, existing strategies and the difficulties and challenges of high-throughput sequencing for molecular diagnosis in genetic diseases are discussed. In part 2, the set up and the technical validation of this diagnostic approach in the Molecular Genetics’ Laboratory of the Timone Hospital in Marseille is presented and illustrated by 3 examples of complex diagnostics solved thanks to NGS. NGS promises to shorten significantly the time of analysis and results reporting, and to expand the number of tested genes. It also promises to increase the proportion of positive diagnoses. Finally, the NGS can identify new variants and new genes involved in human pathology, thus will globally improve patient clinical care
Vining, Nicholas. "Next-generation content creation: an investigative approach." Thesis, 2011. http://hdl.handle.net/1828/3830.
Повний текст джерелаGraduate
Alhomoud, Adeeb M., Irfan U. Awan, Disso Jules F. Pagna, and M. Younas. "A Next Generation Approach to Combating Botnets." 2013. http://hdl.handle.net/10454/13626.
Повний текст джерелаAs part of a defense-in-depth security solution for domain-controlled enterprise networks, a proposed self-healing system architecture is designed to increase resiliency against botnets with minimal disruption to network services.
Doucette, Cody. "An architectural approach for mitigating next-generation denial of service attacks." Thesis, 2021. https://hdl.handle.net/2144/42216.
Повний текст джерела(9593063), Li Cheng. "Laboratory Load-Based Testing, Performance Mapping and Rating of Residential Cooling Equipment." Thesis, 2020.
Знайти повний текст джерелаAlsarhan, Ayoub. "Machine Learning Approach for Spectrum Sharing in the Next Generation Cognitive Mesh Network." Thesis, 2011. http://spectrum.library.concordia.ca/7710/1/Alsarhan_PhD_F2012.pdf.
Повний текст джерелаLiu, Fa-Jen, and 劉發仁. "A homology-based approach for identifying Plant MicroRNAs from Next-Generation Sequencing Data." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5105030%22.&searchmode=basic.
Повний текст джерела國立中興大學
基因體暨生物資訊學研究所
107
MicroRNAs (miRNAs) are short non-coding RNAs with about 22 nt in length. Their main function is to regulate the expression of messenger ribonucleic acids (mRNAs) that can be translated into proteins in organisms. Due to the development of next-generation sequencing (NGS) technology, small RNA sequencing (sRNA-Seq) can be used to help miRNAs prediction. Among the sRNA-Seq-based prediction methods, the reference genome-guided methods express higher precision; on the other hand, most of the genome-free methods utilize the machine learning technology to do prediction, however, the result has a higher false positive rate. This study developed a homology-based approach which is independent of the reference genome to analyze sRNA-Seq data. Previously published sRNA-Seq data of Arabidopsis thaliana were used to test the performance of the new appoach. The results from the new approach were compared with the previously published results by the reference genome-guided method. 83% the miRNA families obtained from the previous study can be identified in the new approach. In addition, the two approaches show similar miRNA expression profiles. We then utilized the new apporach to analyze the miRNAs of Phalaenopsis and Oncidium, and predicted their target genes. In addition, differently expressed miRNAs were identified from different samples and the target genes of most of the differently expressed miRNAs could be predicted. A total of 13 conserved miRNA families, 19 novel miRNA families, and 210 target genes were identified in Phalaenopsis. A total of 17 conserved miRNA families, 1 novel miRNA family, and 35 target genes were identified in the Oncidium. From the miRNA prediction results of Arabidopsis thaliana and the orchids, the feasibility and accuracy of the homology-based approach are demonstrated.Thus this apporach will help to improve the miRNA prediction ability with no reference required, and help researchers to understand the more comprehensive regulatory mechanisms in each organism through miRNA prediction.
Sigel, Erin Mackey. "A Next-Generation Approach to Systematics in the Classic Reticulate Polypodium vulgare Species Complex (Polypodiaceae)." Diss., 2014. http://hdl.handle.net/10161/9092.
Повний текст джерелаThe
Chapter I presents a diploids-only phylogeny of the
Chapter II addresses reported discrepancies regarding the occurrence of
Chapter III examines a case of reciprocal allopolyploid origins in the fern
This dissertation concludes by demonstrating the utility of the allotetraploid
Dissertation
Parameswaran, Aishwarya. "DNA Encoded Libraries (DEGL) of Glycan Antigens to Detect Antibodies: An Approach Towards Next Generation Functional Glycomics." 2017. http://scholarworks.gsu.edu/chemistry_theses/101.
Повний текст джерелаEddyani, M., K. Vandelannoote, Conor J. Meehan, S. Bhuju, J. L. Porter, J. Aguiar, T. Seemann, et al. "A Genomic Approach to Resolving Relapse versus Reinfection among Four Cases of Buruli Ulcer." 2015. http://hdl.handle.net/10454/17297.
Повний текст джерелаBackground. Increased availability of Next Generation Sequencing (NGS) techniques allows, for the first time, to distinguish relapses from reinfections in patients with multiple Buruli ulcer (BU) episodes. Methodology. We compared the number and location of single nucleotide polymorphisms (SNPs) identified by genomic screening between four pairs of Mycobacterium ulcerans isolates collected at the time of first diagnosis and at recurrence, derived from a collection of almost 5000 well characterized clinical samples from one BU treatment center in Benin. Principal Findings. The findings suggest that after surgical treatment—without antibiotics—the second episodes were due to relapse rather than reinfection. Since specific antibiotics were introduced for the treatment of BU, the one patient with a culture available from both disease episodes had M. ulcerans isolates with a genomic distance of 20 SNPs, suggesting the patient was most likely reinfected rather than having a relapse. Conclusions. To our knowledge, this study is the first to study recurrences in M. ulcerans using NGS, and to identify exogenous reinfection as causing a recurrence of BU. The occurrence of reinfection highlights the contribution of ongoing exposure to M. ulcerans to disease recurrence, and has implications for vaccine development.
This work was supported by the UBS Optimus Foundation (Zurich, Switzerland) and the Department of Economy, Science and Innovation of the Flemish Government (Belgium). KV was supported by a VLADOC PhD scholarship of VLIRUOS (Belgium).
Wu, Chih-hsien, and 吳致憲. "Adaptive Load Balancing MDP-based Approach of Two-Dimension Spreading for VSF-OFCDM in Next Generation Cellular Communications." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/14631427024750335633.
Повний текст джерела朝陽科技大學
資訊工程系碩士班
95
The VSF-OFCDM system has been proposed as the forward link interface for achieving high data rate in 4G mobile communications. VSF-OFCDM allocates orthogonal channelization codes of an OVSF code tree in two-dimension (2D) spreading in the time and frequency domains, but suffers from two disadvantages: moderate utilization and low transmission quality. The moderate utilization is caused by code blocking and the low transmission quality is due to the multicode interference from high channel loading in the time domain of the two-dimension spreading. A trade-off exists between code blocking and multicode interference. Specifically, lower the code blocking the system has, higher the interference it yields. To achieve high utilization while providing high transmission quality becomes the critical issue that should be addressed in 4G VSF-OFCDM. Therefore, in this paper, we propose the Adaptive Load-balancing with Markov decision process based 2D spreading approach (i.e., denoted by ALM) for the purpose. The ALM approach consists of three phases. First, for balancing channel load, the adaptive 2D spreading phase is proposed to select that the 2D spreading combinations are with low channel load and high frequency diversity as the candidates. Second, a dynamic re-combination of a 2D spreading is proposed to decrease channel load while supporting transmission quality from high frequency diversity if the channel load of a time domain code exceeds the defined channel load threshold. Third, for minimizing the code blocking, the cost-based Markov decision process code selection approach is adopted. The MDP phase selects the least cost channelization code as the optimal solution if there are several 2D spreading combinations that satisfy the channel load limitation. In addition, the time complexity of ALM is analyzed. Numerical results indicate that the proposed approach outperforms other approaches in transmission quality ratio, fractional reward loss and the total number of reassignments.
Blaschikoff, Ludmilla Paixão. "Dogs (Canis lupus familiaris) from the Iberian Peninsula dated to the Chalcolithic period: a genomic approach." Master's thesis, 2019. http://hdl.handle.net/10773/26896.
Повний текст джерелаDomestic dogs exist in the Iberian Peninsula at least since the Upper Late Palaeolithic; the oldest remain dated to 16,000 BP years old (Erralla, Spain). There are different theories about the origins of European dogs. Previous studies indicated that dogs may arrived in Europe from an Eastern Asia domesticated population of wolves, or that two genetically distinct wolf populations in Eastern and Western Eurasia may have been independently domesticated, and that afterwards the Eastern dog population spread and partially replaced an indigenous Western Eurasian dog population. A recent study focusing in the genetic composition of 6 Mesolithic Iberian dogs reported that a local domestication in the Iberia Peninsula may have occurred in pre-Neolithic Europe. Considering the debated origin of Iberian dogs, it is crucial to unravel the genetic composition of past European peripheral populations - using specific methods to recover and analyse ancient DNA, from different periods in order to further investigate their origins and evolutionary trajectories. Additionally, it may prove important to provide data on a possible contribution of the Iberian wolf to the origin of the first Iberian dogs and genomic information potentially useful for the detection of historical hybridization events between the dog and its wild relative, the Iberian wolf – a subspecies and an endemism currently considered “Endangered”. This information can be included in the definition of future management and conservation measures for the wild Iberian wolf species. In this work, a genomic approach (Next Generation Sequencing, NGS) was carried out to recover mitogenome and nuclear genomic data of Canis from three Iberian archaeological sites dated to the Chalcolithic [ca. 5,000-4,000 years BP], in particular: two dogs from Leceia in Oeiras, Portugal; two dogs from Casetón de La Era in Valladolid, Spain; and one wolf from Penedo de Lexim in Mafra, Portugal. Using the most up-to-date bioinformatic tools, their mitochondrial (mt) and nuclear genomes were sequenced. In addition, to understand the relationship of past/extant populations, a phylogenetic network (based on a partial fragment of the mtDNA control region) comprising 254 Canis sequences, as well as a phylogenetic tree of 23 Canis mitogenomes, publicly available, were constructed. Furthermore, the nuclear genome, although more challenging to recover and analyse from ancient samples, was investigated to molecularly assess the sex of these 5 Canis specimens. Regarding ancient Iberian dogs, this is the first attempt to successfully apply NGS methods to investigate their genomic composition. In this study, it was possible to: generate the draft of mitochondrial genomes (coverages ranged between 1x and 17x) and recover between 0.09% and 3.75% of endogenous nuclear genomic data of these 5 Canis specimens; identify mitochondrial DNA haplotypes and assign those to 2 (A and C) of the four major dog haplogroups described (A, B C and D); generate genetic data from a Chalcolithic wolf - to the best of my knowledge this is the first genomic data available from an Iberian wolf specimen from this chronology. The results shown that the Chalcolithic Iberian dogs had about the same frequency of Haplogroup A (previously present in this territory, but contrasting with other European regions), as well as of the Haplogroup C (already present in other European regions since the Paleolithic).
Mestrado em Ecologia Aplicada
Martins, Sara Patrícia Monteiro. "A metagenomic approach to identify and characterize wastewater populations." Master's thesis, 2017. http://hdl.handle.net/1822/56115.
Повний текст джерелаWater scarcity and pollution are two main ecological focus nowadays. Knowledge of wastewater composition, regarding microorganisms and pollutants, is of great importance to improve the capacities of the effluent treatment plants (ETP). Advances in Next-generation sequencing (NGS) methodologies allowed for faster, cheaper and more accurate study of microbial communities. Besides being an extremely powerful analysis resource, whole shotgun metagenomic analysis comprises many challenging aspects, regarding the processing and analysis. In the present work a shotgun metagenomic bioinformatics analysis was performed comprising three samples from common ETPs (CETP) and four samples from a petrochemical complex ETPs (wastewaters with low and high salts collected in two distinct timepoints). The samples were sequenced with Illumina® HiSeq, generating paired-end reads with 2x150bp length. The main goals of this project were to evaluate currently available tools, establish a customized bioinformatics pipeline and to extract relevant biological information from the sequenced datasets. There were generated simulated datasets representative of the target data, in order to evaluate the performance of the available bioinformatics tools. Datasets were generated with three coverage levels and were used to test pre-processing, assembly and taxonomic tools. The target datasets, both with and without coverage split, were then subjected to processing and analysis using the pre-defined pipeline. A preliminary functional study was also performed using MG-RAST and MGX. Results from the evaluation of the performance of the bioinformatics tools showed that different tools behave differently in distinct datasets. The pipeline was defined using BayesHammer and Fastq-mcf as pre-processing tools, SPAdes for assembly and MetaPhlAn v2.0 for the taxonomical analysis. The assembly results for the target datasets showed a higher contiguity for high coverage levels and a lower contiguity for low coverage levels, highlighting the differences in microorganisms’ abundance and diversity and its impact during analysis. Taxonomical composition suggests the presence of putative pathogenic and opportunistic microorganisms on two of the CETP datasets (A2 and AKR12). It also suggests a more hostile environment in petrochemical complex ETPs datasets, which is concordant with a higher abundance of defence mechanisms on this datasets. The present results must be accounted to the effluent treatment processes.
A escassez de água e a poluição são dois dos principais problemas ecológicos atualmente. O conhecimento da composição das águas residuais, referente a microrganismos e poluentes, é de grande importância para melhorar as capacidades das estações de tratamento de águas residuais (ETAR). Os avanços nos métodos de sequenciação de nova geração permitiram o estudo mais rápido, barato e preciso de comunidades microbianas. Apesar de ser um meio de análise altamente poderoso, a análise metagenómica por whole shotgun compreende muitos aspetos desafiadores, no que respeita o processamento e a análise. No presente trabalho, uma análise bioinformática de dados metagenómicos de shotgun foi efetuada incluindo três amostras de ETARs comuns e quatro amostras de ETARs de um complexo petroquímico (águas residuais com baixos e altos teores de sais, colhidas em dois momentos distintos). As amostras foram sequenciadas com Illumina® HiSeq, gerando paired-end reads com comprimento igual a 2x150pb. Os principais objetivos deste projeto foram avaliar ferramentas disponíveis atualmente, estabelecer uma pipeline bioinformática personalizada e extrair informação biológica relevante dos datasets sequenciados. Foram gerados datasets simulados representativos dos dados a analisar, de forma a avaliar a performance das ferramentas bioinformáticas disponíveis. Os datasets foram gerados com três níveis de coverage e foram usados para testar ferramentas de pré-processamento, assembly e taxonomia. Os datasets alvo, com e sem divisão por coverage, foram então sujeitos a processamento e análise usando a pipeline pré-definida. Um estudo funcional preliminar foi realizado com MG-RAST e MGX. Os resultados da avaliação da performance das ferramentas bioinformáticas mostraram que diferentes ferramentas comportam-se de forma diferente em datasets distintos. A pipeline foi definida usando BayesHammer e Fastq-mcf como ferramentas de pré-processamento, SPAdes para assembly e MetaPhlAn v2.0 para a análise taxonómica. Os resultados de assembly para os datasets alvo mostraram uma grande contiguidade para altos níveis de coverage e baixa contiguidade para baixos níveis de coverage, realçando as diferenças de abundância e diversidade dos microrganismos e o seu impacto durante a análise. A composição taxonómica sugere a presença de microrganismos potencialmente patogénicos e oportunistas nos dois datasets de ETARs comuns (A2 e AKR12). Sugere também um ambiente mais hostil nos datasets das ETARs do complexo petroquímico, o que é concordante com uma maior abundância de mecanismos de defesa nestes datasets. Os presentes resultados devem ser tidos em conta nos processos de tratamento de águas residuais. Palavras-chave: metagenómica de whole shotgun, sequenciação de nova geração, estação de tratamento de águas residuais.
Krahn, Elizabeth. "An autoethnographic study of the legacies of collective trauma experienced by Russian Mennonite women who immigrated to Canada after WWII: implications on aging and the next generation." 2011. http://hdl.handle.net/1993/4821.
Повний текст джерелаZulu, Lindinkosi Lethukuthula. "Simmulating and prototyping software definednetworking (SDN) using Mininet approach to optimise host communication in realistic programmable networking environment." Diss., 2018. http://hdl.handle.net/10500/26156.
Повний текст джерелаElectrical and Mining Engineering
Zulu, Lindinkosi Lethukuthula. "Simulating and prototyping software defined networking (sdn) using mininet approach to optimise host communication in realistic programmable networking environment optimise host communication in realistic programmable networking environment." Diss., 2019. http://hdl.handle.net/10500/25780.
Повний текст джерелаIn this project, two tests were performed. On the first test, Mininet-WiFi was used to simulate a Software Defined Network to demonstrate Mininet-WiFi’ s ability to be used as the Software Defined Network emulator which can also be integrated to the existing network using a Network Virtualized Function (NVF). A typical organization’s computer network was simulated which consisted of a website hosted on the LAMP (Linux, Apache, MySQL, PHP) virtual machine, and an F5 application delivery controller (ADC) which provided load balancing of requests sent to the web applications. A website page request was sent from the virtual stations inside Mininet-WiFi. The request was received by the application delivery controller, which then used round robin technique to send the request to one of the web servers on the LAMP virtual machine. The web server then returned the requested website to the requesting virtual stations using the simulated virtual network. The significance of these results is that it presents Mininet-WiFi as an emulator, which can be integrated into a real programmable networking environment offering a portable, cost effective and easily deployable testing network, which can be run on a single computer. These results are also beneficial to modern network deployments as the live network devices can also communicate with the testing environment for the data center, cloud and mobile provides. On the second test, a Software Defined Network was created in Mininet using python script. An external interface was added to enable communication with the network outside of Mininet. The amazon web services elastic computing cloud was used to host an OpenDaylight controller. This controller is used as a control plane device for the virtual switch within Mininet. In order to test the network, a webserver hosted on the Emulated Virtual Environment – Next Generation (EVENG) software is connected to Mininet. EVE-NG is the Emulated Virtual Environment for networking. It provides tools to be able to model virtual devices and interconnect them with other virtual or physical devices. The OpenDaylight controller was able to create the flows to facilitate communication between the hosts in Mininet and the webserver in the real-life network
The University of South Africa The University of Johannesburg
College of Engineering, Science and Technology
Zulu, Lindinkosi Lethukuthula. "Simulating and prototyping software defined networking (SDN) using Mininet approach to optimise host communication in realistic programmable networking environment." Diss., 2018. http://hdl.handle.net/10500/26218.
Повний текст джерелаElectrical and Mining Engineering
M. Tech. (Electrical Engineering)