Статті в журналах з теми "Nerve tissue Cultures and culture media"
Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: Nerve tissue Cultures and culture media.
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 статей у журналах для дослідження на тему "Nerve tissue Cultures and culture media".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте статті в журналах для різних дисциплін та оформлюйте правильно вашу бібліографію.
1
Pires, Liliana R., Daniela N. Rocha, Luigi Ambrosio, and Ana Paula Pêgo. "The role of the surface on microglia function: implications for central nervous system tissue engineering." Journal of The Royal Society Interface 12, no. 103 (February 2015): 20141224. http://dx.doi.org/10.1098/rsif.2014.1224.
Повний текст джерелаАнотація:
In tissue engineering, it is well accepted that a scaffold surface has a decisive impact on cell behaviour. Here we focused on microglia—the resident immune cells of the central nervous system (CNS)—and on their response to poly(trimethylene carbonate-co-ε-caprolactone) (P(TMC-CL)) fibrous and flat surfaces obtained by electrospinning and solvent cast, respectively. This study aims to provide cues for the design of instructive surfaces that can contribute to the challenging process of CNS regeneration. Cell morphology was evidently affected by the substrate, mirroring the surface main features. Cells cultured on flat substrates presented a round shape, while cells with elongated processes were observed on the electrospun fibres. A higher concentration of the pro-inflammatory cytokine tumour necrosis factor-α was detected in culture media from microglia on fibres. Still, astrogliosis is not exacerbated when astrocytes are cultured in the presence of microglia-conditioned media obtained from cultures in contact with either substrate. Furthermore, a significant percentage of microglia was found to participate in the process of myelin phagocytosis, with the formation of multinucleated giant cells being observed only on films. Altogether, the results presented suggest that microglia in contact with the tested substrates may contribute to the regeneration process, putting forward P(TMC-CL) substrates as supporting matrices for nerve regeneration.
2
Liubich, L. D., L. P. Staino, D. M. Egorova, T. D. Skaterna, and E. G. Pedachenko. "Effect of various origins conditioned media on the migration of neural cells in vitro." Fiziolohichnyĭ zhurnal 68, no. 2 (March 11, 2022): 36–50. http://dx.doi.org/10.15407/fz68.02.036.
Повний текст джерелаАнотація:
An important direction in the development of the latest technologies for the restoration of damaged central nervous system is the use of stem/progenitor cells (SPCs), mainly neurogenic SPCs (NSPCs) and mesenchymal multipotent stromal cells (MMSCs). One of the main mechanisms of SPCs action is indirect paracrine effects due to the ability to produce a wide range of biologically active signaling molecules (secretome). The study of regenerative effects of conditioned media (CM) of NSPCs and MMSCs as a source of their secretome seems to be actual and potentially beneficial. The aim of the study is to compare the impact of CM from 24-h cultures of fetal neurogenic cells (NCs (E14), as a source of NSPCs) and adiposederived mononuclear cells (AMCs as a source of MMSCs) on migration capacity of rat neural cells in vitro. AMCs-CM were obtained from 24-h cultures with prevalence of CD105+ cells and ability upon further cultivation to form “spheroids” and potency to differentiate into different cell types. NCs-CM were obtained from 24-h cultures with prevalence of Nestin+ cells and ability upon further cultivation to form “neurospheres” and potency to differentiate into astrocytes (GFAP+) and neurons (β-Tubulin III+). Rat fetal neural cells (E14) were cultured to achieve a confluent monolayer with basic cellular elements of nervous tissue (5-7th day), which was dissected with forming a transection site and DMEM with 10% fetal calf serum (control) or 0.1-0.3 mg/ml (by total protein amount) of NCs-CM or AMCs-CM were added. In control cultures of rat neural cells partial overgrowth of the dissected area of the monolayer was observed due to the migration of cells, formation of a network of processes and intercellular contacts; reaching 13.2% (4th day) – 23.2% (8th day) of its full length. The overgrown area increased after addition of CM: NCsCM – 3 times (0.1-0.2 mg/ml) and 3-4 times (0.3 mg/ml, 4th-8th day), reaching 70.5% of full length of the transection site; AMCs-CM – 1.5 times (0.1-0.2 mg/ml) and 4-7 times (0.3 mg/ml, 4th-8th day), reaching 97.4-100% of full length of the transection site. The addition of NCs CM and AMCs CM resulted in β-catenin translocation into nucleus of cells in rat neural cell cultures, which correlated with the overgrowth of the transection zone. NCs-CM as well as AMCs-CM in dose-dependent manner stimulate migration processes in culture of rat neural cells, obviously, involving β-catenin signaling pathway, contributing to overgrowing of the dissected area (reparation of a mechanical defect). NCs-CM and AMCs-CM are a source of signaling molecules that modulate the microenvironment and activate endogenous repair mechanisms in culture (in vitro model of nerve tissue regeneration).
3
Saito, Shigeru, Inas Radwan, Hideaki Obata, Kenichiro Takahashi, and Fumio Goto. "Direct Neurotoxicity of Tetracaine on Growth Cones and Neurites of Growing Neurons In Vitro." Anesthesiology 95, no. 3 (September 1, 2001): 726–33. http://dx.doi.org/10.1097/00000542-200109000-00027.
Повний текст джерелаАнотація:
Background Local anesthetics have direct neurotoxicity on neurons. However, precise morphologic changes induced by the direct application of local anesthetics to neurons have not yet been fully understood. Also, despite the fact that local anesthetics are sometimes applied to the sites where peripheral nerves may be regenerating after injury, the effects of local anesthetics on growing or regenerating neurons have never been studied. Methods Three different neuronal tissues (dorsal root ganglion, retinal ganglion cell layer, and sympathetic ganglion chain) were isolated from an age-matched chick embryo and cultured for 20 h. Effects of tetracaine were examined microscopically and by a quantitative morphologic assay, growth cone collapse assay. Results Tetracaine induced growth cone collapse and neurite destruction. Three neuronal tissues showed significantly different dose-response, both at 60 min and at 24 h after the application of tetracaine (P < 0.01). The ED50 values (mean +/- SD) at 60 min were 1.53+/-1.05 mM in dorsal root ganglion, 0.15+/-0.05 mM in retinal, and 0.06+/-0.02 mM in sympathetic ganglion chain cultures. The ED50 values at 24 h were 0.43+/-0.15 mM in dorsal root ganglion, 0.07+/-0.03 mM in retinal, and 0.02+/-0.01 mM in sympathetic ganglion chain cultures. Concentration of nerve growth factor in the culture media did not influence the ED50 values. The growth cone collapsing effect was partially reversible in dorsal root ganglion and retinal neurons. However, in the sympathetic ganglion culture, no reversibility was observed after exposure to 1 mM tetracaine for 10 or for 60 min. Bupivacaine had similar neurotoxicity to the three types of growing neurons. (The ED50 values at 60 min were 2.32+/-0.50 mM in dorsal root ganglion, 0.96+/-0.16 mM in retinal, and 0.18+/-0.05 mM in sympathetic ganglion chain cultures. The ED50 values at 24 h were 0.34+/-0.09 mM in dorsal root ganglion, 0.21+/-0.06 mM in retinal, and 0.45+/-0.10 mM in sympathetic ganglion chain cultures.) Conclusions Short-term exposure to tetracaine produced irreversible changes in growing neurons. Growth cones were quickly affected, and neurites degenerated subsequently. Sensitivity varied with neuronal type and was not influenced by the concentration of nerve growth factor. Because a similar phenomenon was observed after exposure to bupivacaine, the toxicity to growing neurons may not be unique to tetracaine.
4
Walum, Erik, Elisabeth Hansson, and Alan L. Harvey. "In Vitro Testing of Neurotoxicity." Alternatives to Laboratory Animals 18, no. 1_part_1 (November 1990): 153–79. http://dx.doi.org/10.1177/026119299001800118.1.
Повний текст джерелаАнотація:
Many of the toxic compounds that are at large in the environment represent a risk to our neuronal functions. Chemicals may have a direct or indirect effect on the nervous system and they may interfere with general biochemical properties or specific neuronal structures and processes. In this review, a brief presentation of the major neurotoxicological targets is given, together with a discussion of some aspects of the use of different in vitro models for screening purposes and mechanistic studies. It is believed that in vitro methods offer special opportunities for the development of new neurotoxicological assays, and that this development will mainly involve cultured model systems. Therefore, a presentation of nerve and glia tissue culture methods is given, followed by an overview of how information on the action of mercury and mercurials, excitotoxins and acrylamide has been obtained through the use of cultured cell models. It is concluded that the developmental potential in cell neurotoxicology lies within the areas of separation and identification of cells representative for different structures in the nervous system, co-cultivation of different cell types, in vivo/in vitro (ex vivo) procedures, chemically defined media, metabolic competent cultures of human cells and improved physiological conditions for cultivation and exposure.
5
Zachos, Nicholas C. "Abstract IA008: Modeling immune-epithelial interactions in health and disease using human intestinal enteroid co-cultures." Cancer Research 82, no. 23_Supplement_1 (December 1, 2022): IA008. http://dx.doi.org/10.1158/1538-7445.crc22-ia008.
Повний текст джерелаАнотація:
Abstract Human intestinal enteroids derived from Lgr5+ actively dividing adult stem cells offer a relevant ex vivo system to study biological processes of the human small intestine and colon. While these primary cultures recreate cellular and functional features of the intestinal epithelium of the small intestine (enteroids) or colon (colonoids), they are limited by the lack of associated cell types, including immunological cells, nerves, stromal cells that help maintain tissue homeostasis and respond to external challenges. In the gut, innate immune cells interact with the epithelium by conducting lumial surveillance, support barrier function, and deploy effector functions in response to injury/damage. We have established a co-culture system of enteroid/colonoid monolayers and underlying macrophages and polymorphonuclear neutrophils to recapitulate the cellular framework of the human intestinal epithelial niche. Human intestinal enteroids can generated from biopsies or resected tissue from any segment of the human intestine/colon and maintained in long-term cultures in a basement membrane-rich matrix supplemented with growth factor-conditioned media necessary to maintain intestinal stem cell homeostasis. Immune cells are isolated from fresh human whole blood or frozen peripheral blood mononuclear cells (PBMC) and co-cultured with human intestinal enteroids/colonoids grown as confluent monolayers on permeable supports (e.g., Transwells). This configuration allows for controlled access to the apical side of the epithelium to mimic the gut luminal environment as well as the basolateral side occupied by immune cells to mimic the mucosal environment. Enteroid-immune co-cultures enable multiple outcome measures, including transepithelial resistance, production of cytokines/chemokines, phenotypic analysis of immune cells, tissue immunofluorescence imaging, protein or mRNA expression, antigen or microbe uptake, and other cellular functions. Citation Format: Nicholas C. Zachos. Modeling immune-epithelial interactions in health and disease using human intestinal enteroid co-cultures [abstract]. In: Proceedings of the AACR Special Conference on Colorectal Cancer; 2022 Oct 1-4; Portland, OR. Philadelphia (PA): AACR; Cancer Res 2022;82(23 Suppl_1):Abstract nr IA008.
6
Lischer, Mirko, Pietro G. di Summa, Carlo M. Oranges, Dirk J. Schaefer, Daniel F. Kalbermatten, Raphael Guzman, and Srinivas Madduri. "Human platelet lysate stimulated adipose stem cells exhibit strong neurotrophic potency for nerve tissue engineering applications." Regenerative Medicine 15, no. 3 (March 2020): 1399–408. http://dx.doi.org/10.2217/rme-2020-0031.
Повний текст джерелаАнотація:
Aim: We investigated a potential strategy involving human platelet lysate (HPL) as a media additive for enhancing the neurotrophic potency of human adipose stem cells (ASC). Materials & methods: Dorsal root ganglion explants, ASC and Schwann cells were used for in vitro axonal outgrowth experiments. Results: Remarkably, HPL-supplemented ASC promoted robust axonal outgrowth, in other words, four-times higher than fetal bovine serum-supplemented ASC and even matched to the level of Schwann cells. Further, analysis of regime of growth medium additive supplementation revealed the critical play of HPL in dorsal root ganglion and stem cells co-culture system for mounting effective axonal growth response. Conclusion: HPL supplementation significantly improved the neurotrophic potency of ASC as evidenced by the robust axonal outgrowth; these findings hold significance for nerve tissue engineering applications.
7
Meftahpour, Vafa, Somaiyeh Malekghasemi, Amir Baghbanzadeh, Ali Aghebati-Maleki, Ramin Pourakbari, Ali Fotouhi, and Leili Aghebati-Maleki. "Platelet lysate: a promising candidate in regenerative medicine." Regenerative Medicine 16, no. 1 (January 2021): 71–85. http://dx.doi.org/10.2217/rme-2020-0065.
Повний текст джерелаАнотація:
Human platelet lysate has attracted much interest from many researchers as it is growth-factor rich for cell expansion, which is employed as a new therapeutic strategy. Not only are human platelet lysates used for cell therapy, but they are also used for the completion of basal media in mesenchymal stem cell cultures. Due to the presence of a large number of growth factors, platelet lysates have potential roles in wound healing, treatment of ocular graft-versus-host disease, osteoarthritis, Parkinson’s disease, tendon regeneration, infertility, androgenetic alopecia, nerve repair and regenerative tissue, such as bone regeneration. In this review, we summarize that platelet lysates could be valuable candidates for the treatment of a variety of diseases in regenerative medicine.
8
Хасанов, Р. Р., А. А. Гумеров, К. Х. Шефер, and Л. М. Вессель. "A method for culturing of enteric nervous system cells suitable for tissue engineering of the intestine." ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia», no. 2() (May 27, 2019): 132–41. http://dx.doi.org/10.25557/0031-2991.2019.02.132-141.
Повний текст джерелаАнотація:
Лечение синдрома короткой кишки является сложной проблемой в современной медицине. У ряда пациентов существующие методы лечения неэффективны, а трансплантация кишечника показывает неудовлетворительные результаты. Тканевая инженерия тонкой кишки может быть инновационным методом лечения этих пациентов. Одним из ключевых элементов в создании кишки является выращивание нервной системы кишечника. До настоящего времени не описаны культуры нервных клеток, выделенных из нервной системы кишечника, выращенные в трёхмерном матриксе на основе гиалуроновой кислоты. Цель исследования: разработать метод выращивания in vitro взаимодействующих друг с другом клеток нервной системы кишечника в трехмерной среде. Методы: клетки нервной системы кишечника выделялись из кишечника крыс путём препарирования и ферментативного воздействия на мышечный слой тонкой кишки. Далее клетки помещались в трёхмерный матрикс и культивировались в нём в течении нескольких дней. Результаты. Клетки нервной системы кишечника были выделены из крыс и культивировались в трёхмерном матриксе, помещённом в раствор для культур клеток с добавлением специфических факторов роста. Микроскопирование культур клеток на восьмые сутки показало, что нервные клетки активно растут, соединяясь с другими нервными клетками при помощи отростков. Конфокальная микроскопия в сочетании с иммуногистохимическим окрашиванием клеток специфичным маркером нервных клеток показала, что полученные культуры действительно состоят из нервных клеток. Заключение. Разработанный метод позволяет вырастить in vitro живущие и взаимодействующие друг с другом клетки нервной системы кишечника в трехмерной среде. Полученная культура клеток может быть использована для моделирования стенки тонкой кишки путем тканевой инженерии. Background. Treatment of short bowel syndrome is a complex issue in modern medicine. Existing treatment methods are inefficient in some cases, and bowel transplantation shows unsatisfactory results. Tissue engineering of the small intestine can represent an innovative method of treatment for these patients. One of the key elements in creation of the gut is culturing of enteric nervous system cells. There is no description of the nerve cells culture extracted from the enteric nervous system and grown in a hyaluronic-acid based three-dimensional matrix so far. Research objective: establishing an in vitro method for culturing of interacting enteric nervous system cells in a 3D environment. Methods. The enteric nervous system cells used for these experiments were isolated from Sprague-Dawley rats. Hyaluronic-acid based hydrogel HyStem®-C (ESI BIO - A Division of BioTime, USA) was used as a 3D matrix. Enteric nervous system cells were isolated from 5- to 8-day-old newborn Sprague-Dawley rats. Animals were decapitated with a rodent guillotine (cervical spinal cord transection). After that they were laparotomized, and their intestines were isolated. The removed intestines were placed in a Petri dish filled with the MEM (Minimum Essential Medium) supplemented with antibiotics (25 µl factory-produced solution of gentamycin (40 mg/ml) and 50 µl of metronidazole solution (5 mg/ml) per 50 ml of MEM). The small intestine was then separated from the colon and mesentery under an optical microscope, and the muscular layer of small intestine was isolated from the submucosal layer. The isolated muscular layer was placed in a tube with the deoxyribonuclease and collagenase solution as well as Balanced Salt Solution and incubated for 2 h at 37°C and 5% CO2. As a result, the intestinal muscle tissue was broken down completely in the produced solution, while areas of the myenteric plexus remained intact and were recognizable under the microscope as a network. These neural networks were treated with a trypsin solution. Further, after mechanical processing, a suspension of nerve cells was obtained. The hyaluronic-acid based gel HyStem®-C (ESI BIO - A Division of BioTime, Inc., USA) with added collagen was used to produce a 3D matrix. The enteric nervous system cells were added to this three-dimensional matrix and stirred. Matrix had been hardening for 30 minutes. The cell cultures were fixed, stained and microscoped 10-21 days later. For immunofluorescence staining of cells we used a direct immunohistochemical method with the anti-ß III Tubulin antibody conjugated with the fluorochrome Alexa 488 Flour (Merck Millipore). Anti-ß III Tubulin is a specific antibody used for peculiar staining of neurons. The anthraquinone dye with a high affinity to double-stranded DNA - DRAQ5 (Thermo Fisher Scientific) was applied in order to identify cell nuclei. Microscopy was conducted with Leica TCS SP8 (Leica, Germany). Results. The technique we represented allowed us to produce a culture of enteric nervous system cells isolated from rats in the three-dimensional matrix. Confocal microscopy combined with immunohistochemical staining with specific neuronal marker showed that the final cultures indeed consisted of nerve cells. Larger nerve cell clusters were encountered. Herewith, some neurons were arranged close to each other while others were located at a distance; however, all neurons were interconnected. Neurons also clearly showed wide processes morphologically similar to those of axons. In addition, many thin and branching processes resembling dendrites morphologically were visible. The use of confocal microscopy allowed to receive a series of images at different depths of the focal plane, which could then be reconstructed to a three-dimensional image. The 3D reconstruction of the nervous plexi clearly revealed neurons joined together in a complex network. For DRAQ5 stains all cell nuclei, not just those of neurons, the presence of stained cell nuclei just “hanging” on the reconstruction without cytosomes of nerve cells indicated that other cell types were also present in this texture. The present work showed a possibility for culturing interconnected enteric nervous system cells as well as nerve plexi within the three-dimensional medium in vitro. The key step in growing the enteric nervous system cells for both two-dimensional and three-dimensional medium claims to be the characteristics of functional cellular activity. Following stages of the study require observing of the cells’ functionality, for example, by using the analysis of intracellular Ca 2+ concentration (calcium imaging) and its variability. In addition, the study of cellular interactions within the enteric nervous system with other types of cells in the three-dimensional medium also encourages a great interest. Conclusion. The developed method enables one to culture the interconnected cells of the enteric nervous system within the three-dimensional medium in vitro, which can be used to create the enteric nervous system when growing the small intestinal wall by means of tissue engineering. Further research is based on studying the functionality of cells grown in a three-dimensional medium as well as on co-culturing of enteric nerve cells with other types of cells in three-dimensional media.
9
Basu, Anustup. "The Passion of the Digital: the Ontology of the Photographic Image in the Age of New Media." Recherches sémiotiques 31, no. 1-2-3 (November 20, 2014): 175–202. http://dx.doi.org/10.7202/1027447ar.
Повний текст джерелаАнотація:
Using Mel Gibson’s 2004 film and cultural phenomenonThe Passion of the Christas a launching pad, this essay meditates on some questions about the twentieth century legacy of competing realisms, the graphic imperative of contemporary digital image cultures, and the ontological conundrums involving technology and mass media.Passionis an onto-theological filmic ‘event’ that derives equally from an almighty religiosity as well as a cultish process of being enraptured by certain ritual values of a new-age technologism of sound and image. This endographic writing out of the Gospel narrative at the level of the tissue and nerve of the committed viewer affirms a transcendental truth already there in an internal cosmos of belief instead of working in terms of an externally navigable ‘realist’ representation of the world that seeks to ‘bear away our faith’. This is rendered possible when unquestioning belief in Christ and in his momentous sacrifice is met by an embracing of technologywithout the skepticism of a scientific temper.
10
Seil, Fredrick J. "Tissue Culture Models of Myelination After Oligodendrocyte Transplantation." Journal of Neural Transplantation 1, no. 2 (1989): 49–55. http://dx.doi.org/10.1155/np.1989.49.
Повний текст джерелаАнотація:
Studies of myelination after transplantation of mature oligodendrocytes to cerebellar cultures in which oligodendrocyte maturation and myelination had been irreversibly inhibited by exposure to cytosine arabinoside were reviewed. Transplanted oligodendrocytes were derived from three sources, including cerebellar explants treated with kainic acid, dissociated oligodendrocyte cultures, and optic nerve fragments. Oligodendrocytes from all sources migrated into the host explants and myelinated appropriate axons. The time of appearance of myelin and the percentage of host cultures myelinated differed for the three sources of oligodendrocytes, however. Myelin was visible earliest and in the highest percentage of host explants transplanted with cultured dissociated oligodendrocytes, which were presumably the most free to migrate into the host tissue, and latest and in the lowest percentage of host cultures transplanted with optic nerve, from which oligodendrocytes were presumably least free to migrate. Some myelin-like membranes unassociated with axons appeared in cerebellar cultures transplanted with cultured dissociated oligodendrocytes, and not in cerebellar explants transplanted with oligodendrocytes from other sources. The formation of such myelin-like membranes was interpreted as a manifestation of oligodendrocyte hyperreactivity induced by culture in isolation.
11
Quirici, Nadia, Nicoletta Del Papa, Cinzia Scavullo, Michela Cortiana, Chiara Borsotti, Wanda Maglione, Denise Comina, Agostino Cortelezzi, Giorgio Lambertenghi Deliliers, and Davide Soligo. "Stem Cells Defects in Systemic Sclerosis." Blood 106, no. 11 (November 16, 2005): 4194. http://dx.doi.org/10.1182/blood.v106.11.4194.4194.
Повний текст джерелаАнотація:
Abstract Systemic Sclerosis (SSc) is a connective tissue disease characterized by early generalized microangiopathy and culminating in systemic fibrosis. Recent studies have provided evidence that SSc is associated with a reactive but ineffective angiogenesis, so that the disease finally leads to the irreversible loss of capillaries. Aim of the study was to investigate whether impaired vasculogenesis in SSc is due to defective characteristics in BM microenvironment. Peripheral blood (PB) samples were collected from 70 patients (pts): circulating endothelial progenitors (CEPs) were characterized as CD45−/CD133+ and evaluated by flow cytometry. BM samples were collected from 14 SSc pts and hematopoiesis evaluated by various assays. CD133+ cells were isolated by immunomagnetic sorting (IMS) and grown in order to induce endothelial differentiation. Long-term bone marrow cultures (LTBMC) were assessed and the number of stromal clonogenic precursors evaluated by a CFU-F (colony-forming unit fibroblast) assay. Mesenchymal stem cells (MSC) were separated by IMS for the expression of the nerve growth factor-receptor (NGF-R+) and grown in order to assess the clonogenic potential and the proliferative capacity, while their multipotential differentiation ability was determined after culture in different conditioned media. Phenotypic analysis of BM mononuclear cells showed a greater expression of the surface markers P1H12 and CD105 TGF-β receptor (1.2%±0.6 vs 0.5%±0.1 in normal controls, p=0.01 and 9.9%±5 vs 4.7%±3, p=0.02 respectively), but lower percentages of NGF-R+ stromal cell precursors (0.73±0.5 vs 1.61±0.6, p=0.02) and CD133+ cells (0.36%±0.4 vs 1.2%±0.8, p=0.05). On the contrary, the absolute number of CEPs in PB was higher in patients with SSc than in healthy controls (mean values 2.1 cells/μL vs 0.26 cells/μL, p=0.04). When BM CD133+ cells were grown in the presence of VEGF, only 3/12 cases gave endothelial differentiation, but always with a reduced proliferative ability. All pts showed a defective stromal compartment and a reduced number of BM stromal precursors, as detected by the LTBMC and by the lower CFU-F frequency (4%±3.2 vs 43%±19.8/1x10(e)6 LDMNCs, p=0.002 and 7±12.8 vs 69±61/1x10(e)5 NGF-R+ cells, p=0.01). Interestingly, NGF-R+ MSC overexpressed KDR and CD117 (26.4%±7.4 vs 4.6%±1.7, p=0.01 and 87.7%±5.1 vs 57.6%±11, p=0.03 respectively): when grown in the presence of VEGF they gave rise to endothelial colonies, only in 2/8 cases they formed a confluent layer with fibroblastic morphology but a reduced proliferative ability, while in the presence of adipogenic or osteogenic inductive media they failed to origin specific differentiation. Moreover, all “in vitro” differentiated endothelial cells even before activation showed high levels of CD62-E, VCAM-1 and CD105 expression, suggestive of the presence of increased levels of proangiogenic factors in BM. The results of this study provide evidence that patients with SSc have a stem cell defect involving both the hematopoietic and the stromal cells compartments. The higher expression of KDR on NGF-R+ cells suggests a role for VEGF in inducing endothelial differentiation of MSC, so resulting in a depletion of stromal precursors. The continuous recruitment of endothelial progenitors to sites of vascular injury, suggested by the high numbers of CEPs in PB, might lead to the irreversible BM damage we observed.
12
Merkle, S. A., and H. E. Sommer. "Somatic embryogenesis in tissue cultures of Liriodendrontulipifera." Canadian Journal of Forest Research 16, no. 2 (April 1, 1986): 420–22. http://dx.doi.org/10.1139/x86-077.
Повний текст джерелаАнотація:
Tissue cultures of yellow poplar (Liriodendrontulipifera L.) were initiated from immature and mature zygotic embryos. Nodular embryogenic callus developed from a low percentage of the cultures initiated from immature embryos on solid media supplemented with 2,4-dichlorophenoxyacetic acid, 6-benzyladenine, and casein hydrolysate. Embryoids differentiated from these culture lines within 1 month following transfer of embryogenic callus to hormone-free solid media. Although most embryoids appeared abnormal, embryoids with well-formed cotyledons and radicles were capable of developing into normal plantlets.
13
Herring, April S., and R. Daniel Lineberger. "340 The Plant Tissue Culture Information Exchange Media Database." HortScience 35, no. 3 (June 2000): 450E—450. http://dx.doi.org/10.21273/hortsci.35.3.450e.
Повний текст джерелаАнотація:
The Univ. of Minnesota hosts the PLANT-TC Listserv as a service to the international tissue culture community (http://www.agro.agri.umn.edu/plant-tc/listserv/). One of the most frequently sought types of information is a recommendation for a “beginning point” for culturing a wide variety of plant species. Many of these inquiries come from individuals without ready access to extensive library holdings, including those in industry, public schools, and international sites. A Web site prototype that includes a searchable database of tissue culture recipes is being constructed and offered for user input. The database currently is located at http://webtutor.tamu.edu/students/herring/project/, but will be redirected to its own URL if user feedback is positive. The database also includes information about equipment and materials, media suppliers and domestic and foreign sources for tissue cultures and micropropagated plants. Other educational resources, including a virtual tour of a commercial tissue culture lab, are available on the site. The Web site and database will be reviewed by a panel of experts and modified according to their input prior to being posted for public access.
14
Mothersill, Carmel, Colin Seymour, M. J. Moriarty, and M. J. Cullen. "Long-term culture of differentiated human thyroid tissue." Acta Endocrinologica 108, no. 2 (February 1985): 192–99. http://dx.doi.org/10.1530/acta.0.1080192.
Повний текст джерелаАнотація:
Abstract. Human thyroid cells obtained during surgery have been maintained in monolayer culture for at least 2 months and without loss of morphological or functional differentiation. Samples as small as 0.5 g could be cultured but best results were obtained with samples of 5–10 g. The technique used was developed in this laboratory for sheep tissue and was applicable without significant modification to human tissue. It depends on the complete absence of media changes at any time during the culture period. Energy substrates are replenished by the addition of concentrated glucose solutions to the existing media at carefully monitored intervals. Differences in both morphology and function could be observed between cultures derived from patients with different diseases, suggesting that the technique could have predictive value.
15
Pastuła, Agnieszka, Moritz Middelhoff, Anna Brandtner, Moritz Tobiasch, Bettina Höhl, Andreas H. Nuber, Ihsan Ekin Demir, et al. "Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche." Stem Cells International 2016 (2016): 1–16. http://dx.doi.org/10.1155/2016/3710836.
Повний текст джерелаАнотація:
The gastrointestinal epithelium is characterized by a high turnover of cells and intestinal stem cells predominantly reside at the bottom of crypts and their progeny serve to maintain normal intestinal homeostasis. Accumulating evidence demonstrates the pivotal role of a niche surrounding intestinal stem cells in crypts, which consists of cellular and soluble components and creates an environment constantly influencing the fate of stem cells. Here we describe different 3D culture systems to culture gastrointestinal epithelium that should enable us to study the stem cell nichein vitroin the future: organoid culture and multilayered systems such as organotypic cell culture and culture of intestinal tissue fragmentsex vivo. These methods mimic thein vivosituationin vitroby creating 3D culture conditions that reflect the physiological situation of intestinal crypts. Modifications of the composition of the culture media as well as coculturing epithelial organoids with previously described cellular components such as myofibroblasts, collagen, and neurons show the impact of the methods applied to investigate niche interactionsin vitro. We further present a novel method to isolate labeled nerves from the enteric nervous system using Dclk1-CreGFP mice.
16
Tisserat, Brent. "Growth Responses and Construction Costs of Various Tissue Culture Systems." HortTechnology 6, no. 1 (January 1996): 62–68. http://dx.doi.org/10.21273/horttech.6.1.62.
Повний текст джерелаАнотація:
The influence of the culture chamber size and medium volume on the growth rates of shoot tips of peas, lettuce, kidney beans, and spearmint were determined after 8 weeks of incubation. Cultures were grown in a variety of culture chambers including culture tubes, baby food jars, Magenta GA-7 containers, 1-pint Mason jars, 1-quart Mason jars used with and without an automated plant culture system (APCS), 0.5-gal Mason jars with and without an APCS, Bio-safe chambers with an APCS, and polycarbonate culture chambers with an APCS having culture chamber volumes of 55, 143, 365, 462, 925, 1850, 6000, and 16,400 ml, respectively. Plans are presented for the construction of various culture chambers used in an APCS. The APCS consisted of a peristaltic pump, media reservoir containing 1 liter of liquid nutrient medium, and a culture chamber. Cultures grown with an APCS consistently produced higher fresh weights than cultures using any of the agar culture systems tested. Growth rates varied considerably depending on the plant species and culture system tested. Peas, lettuce, and spearmint exhibited flowering only when grown in the APCS. A cost comparison using the APCS versus various conventional tissue culture systems is presented.
17
Kim, G. J., S. Yoo, S. Han, J. Bu, Y. Hong, and D.-K. Kim. "Bacterial strain changes during chronic otitis media surgery." Journal of Laryngology & Otology 131, no. 9 (July 11, 2017): 801–4. http://dx.doi.org/10.1017/s0022215117001414.
Повний текст джерелаАнотація:
AbstractObjective:Cultures obtained from pre-operative middle-ear swabs from patients with chronic otitis media have traditionally been used to guide antibiotic selection. This study investigated changes in the bacterial strains of the middle ear during chronic otitis media surgery.Methods:Pre-operative bacterial cultures of otorrhoea, and peri-operative cultures of the granulation tissue in either the middle ear or mastoid cavity, were obtained. Post-operative cultures were selectively obtained when otorrhoea developed after surgery.Results:Bacterial growth was observed in 45.5 per cent of pre-operative cultures, 13.5 per cent of peri-operative cultures and 4.5 per cent of post-operative cultures. Methicillin-resistant Staphylococcus aureus was identified as the most common bacteria in all pre-operative (32.4 per cent), peri-operative (52.4 per cent) and post-operative (71.4 per cent) tests, and the percentage of Methicillin-resistant S aureus increased from the pre- to the post-operative period.Conclusion:The bacterial culture results for post-operative otorrhoea showed low agreement with those for pre-operative or peri-operative culture, and strain re-identification was required.
18
Jung, Jieun, Jong-Wan Kim, Ho-Jin Moon, Jin Young Hong, and Jung Keun Hyun. "Characterization of Neurogenic Potential of Dental Pulp Stem Cells Cultured in Xeno/Serum-Free Condition:In VitroandIn VivoAssessment." Stem Cells International 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/6921097.
Повний текст джерелаАнотація:
Neural stem cells (NSCs) have a high potency for differentiation to neurons and glial cells for replacement of damaged cells and paracrine effects for the regeneration and remyelination of host axons. Dental pulp is known to have a potential to differentiate into neural-like cells; therefore, dental pulp may be used as an autologous cell source for neural repair. In this study, we selectively expanded stem cells from human dental pulp in an initial culture using NSC media under xeno- and serum-free conditions. At the initial step of primary culture, human dental pulp was divided into two groups according to the culture media: 10% fetal bovine serum medium group (FBS group) and NSC culture medium group (NSC group). In the NSC group relative to the FBS group, the expression of NSC markers and the concentrations of leukemia inhibitory factor, nerve growth factor, and stem cell factor were higher, although their expression levels were lower than those of human fetal NSCs. The transplanted cells of the NSC group survived well within the normal brain and injured spinal cord of rats and expressed nestin and Sox2. Under the xeno- and serum-free conditions, autologous human dental pulp-derived stem cells might prove useful for clinical cell-based therapies to repair damaged neural tissues.
19
Stapor, Peter C., Mohammad S. Azimi, Tabassum Ahsan, and Walter L. Murfee. "An angiogenesis model for investigating multicellular interactions across intact microvascular networks." American Journal of Physiology-Heart and Circulatory Physiology 304, no. 2 (January 15, 2013): H235—H245. http://dx.doi.org/10.1152/ajpheart.00552.2012.
Повний текст джерелаАнотація:
Developing therapies aimed at manipulating microvascular remodeling requires a better understanding of angiogenesis and how angiogenesis relates to other network remodeling processes, such as lymphangiogenesis and neurogenesis. The objective of this study was to develop an angiogenesis model that enables probing of multicellular and multisystem interactions at the molecular level across an intact adult microvascular network. Adult male Wistar rat mesenteric windows were aseptically harvested and cultured in serum-free minimum essential media. Viability/cytotoxicity analysis revealed that cells remain alive for at least 7 days. Immunohistochemical labeling at 3 days for platelet endothelial cell adhesion molecule (PECAM), neuron-glial antigen 2 (NG2), lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), and class III β-tubulin identified endothelial cells, pericytes, lymphatics, and nerves, respectively. Media supplemented with bFGF or VEGF induced an increase in endothelial cell sprouting off existing vessels. Endothelial cell sprouting in both growth factor groups was inhibited by targeting pericytes with NG2 functional blocking antibody. VEGF caused an increase in the number of lymphatic/blood endothelial cell connections compared with media alone or bFGF groups. Finally, the comparison of the same network before and after angiogenesis stimulated by the supplement of media with 20% serum identified the ability of disconnected endothelial segments to reconnect to nearby vessels. The results establish a novel in situ angiogenesis model for investigating the location of capillary sprouting within an intact network, the role of pericytes, lymphatic/blood endothelial cell interactions, and the fate of specific endothelial cell segments. The rat mesentery culture system offers a unique tool for understanding the complex dynamics associated with angiogenesis in an intact adult tissue.
20
Seeber, Judith W., Michaela Zorn-Kruppa, Simone Lombardi-Borgia, Heike Scholz, Anna K. Manzer, Brigitte Rusche, Monika Schäfer-Korting, and Maria Engelke. "Characterisation of Human Corneal Epithelial Cell Cultures Maintained under Serum-free Conditions." Alternatives to Laboratory Animals 36, no. 5 (November 2008): 569–83. http://dx.doi.org/10.1177/026119290803600512.
Повний текст джерелаАнотація:
Three-dimensional tissue constructs have been proposed as in vitro screening models for ocular irritancy. Based on our previous studies, in which a full-thickness corneal model based exclusively on SV40-immortalised cell lines was generated, we have currently evaluated the effects of a range of commercially-available cell culture media on several cellular parameters in cultures of a human corneal epithelial (HCE) cell line. This cell line was used in an attempt to establish a rational basis for the development of serum-free culture media for the assembly and long-term tissue culture of full-thickness corneal models. Briefly, we investigated the impact of serum-free culture on the proliferation, morphology, barrier function and cytokine expression of HCE cells. The number of cell layers and the epithelial differentiation were evaluated by histology. Barrier properties were characterised via the determination of transepithelial electrical resistance (TEER), fluorescein permeation, and the expression of the tight junction-related protein, zona occludin 1 (ZO-1). The cytokine expression pattern in response to serum-free culture was measured by using an antibody array system. Our results revealed that both the morphology and the barrier function of the epithelial constructs were comparable to those of human donor corneas, when serum-free media were supplemented with ascorbic acid, calcium, hydrocortisone and retinoic acid. Under these conditions, the artificial epithelium based on serum-free HCE cultures represented a valid model for the natural ocular surface.
21
Ptak, Louis R., Katherine R. Hart, Donghui Lin, and Paul M. Carvey. "Isolation and Manipulation of Rostral Mesencephalic Tegmental Progenitor Cells from Rat." Cell Transplantation 4, no. 3 (May 1995): 335–42. http://dx.doi.org/10.1177/096368979500400312.
Повний текст джерелаАнотація:
A technique for isolating mitotic progenitor cells from the embryonic rostral mesencephalon is described. Culture of the progenitor cells in complete media with subsequent staining for neuron specific enolase (NSE) revealed that only 0.6% of the cells were NSE immunoreactive. Coculturing the progenitor cells with established striatal cultures did not result in conversion of any of the cells to the dopamine neuron phenotype (tyrosine hydroxylase immunoreactive (THir) neurons). In contrast, co-culture of progenitor cells with established mesencephalic cultures produced a statistically significant, and in some cases (three of twelve), dramatic increase in the number of THir cells. The THir cells that were present had more pronounced process extension than those observed in mesencephalic mono-cultures. Culturing progenitor cells in transwell baskets that were continuously exposed to media but physically separated from established mesencephalic cultures growing underneath the baskets led to the conversion of only a few progenitor cells to THir neurons in four of twelve transwell studies suggesting that cell-cell contact between progenitor cells and mesencephalic cells is required for the conversion. This co-culture technique also increased the number of THir neurons in the mesencephalic cultures although the increase was not profound enough to explain the increase observed in traditional co-culture. These data suggest that mitotic progenitor cells can be isolated from fetal rat tissue and successfully converted to the dopamine neuron phenotype. Progenitor-mesencephalic co-culture appears to increase the number of THir cells in both tissue sources mediated in part by soluble factor(s) although cell-cell contact and presumably extracellular matrix proteins play a more substantial role. These progenitor cells may prove useful as a tissue source for transplantation procedures in Parkinson's disease.
22
Dudman, Joseph, Ana Marina Ferreira, Piergiorgio Gentile, Xiao Wang, and Kenneth Dalgarno. "Microvalve Bioprinting of MSC-Chondrocyte Co-Cultures." Cells 10, no. 12 (November 27, 2021): 3329. http://dx.doi.org/10.3390/cells10123329.
Повний текст джерелаАнотація:
Recent improvements within the fields of high-throughput screening and 3D tissue culture have provided the possibility of developing in vitro micro-tissue models that can be used to study diseases and screen potential new therapies. This paper reports a proof-of-concept study on the use of microvalve-based bioprinting to create laminar MSC-chondrocyte co-cultures to investigate whether the use of MSCs in ACI procedures would stimulate enhanced ECM production by chondrocytes. Microvalve-based bioprinting uses small-scale solenoid valves (microvalves) to deposit cells suspended in media in a consistent and repeatable manner. In this case, MSCs and chondrocytes have been sequentially printed into an insert-based transwell system in order to create a laminar co-culture, with variations in the ratios of the cell types used to investigate the potential for MSCs to stimulate ECM production. Histological and indirect immunofluorescence staining revealed the formation of dense tissue structures within the chondrocyte and MSC-chondrocyte cell co-cultures, alongside the establishment of a proliferative region at the base of the tissue. No stimulatory or inhibitory effect in terms of ECM production was observed through the introduction of MSCs, although the potential for an immunomodulatory benefit remains. This study, therefore, provides a novel method to enable the scalable production of therapeutically relevant micro-tissue models that can be used for in vitro research to optimise ACI procedures.
23
Kałużna, Monika, Artur Mikiciński, Piotr Sobiczewski, Marta Zawadzka, Elżbieta Zenkteler, and Teresa Orlikowska. "Detection, isolation, and preliminary characterization of bacteria contaminating plant tissue cultures." Acta Agrobotanica 66, no. 4 (2014): 81–92. http://dx.doi.org/10.5586/aa.2013.054.
Повний текст джерелаАнотація:
In order to limit the contamination problem in plant tissue cultures experiments on selection of media suitable for detection and isolation of bacteria contaminating plant tissue explants, and preliminary characterization of isolates were made. In the first experiment aiming at detection of bacteria in plant explants four strains representing genera most often occurring at our survey of plant tissue cultures, and earlier isolated and identified (<em>Bacillus, Methylobacterium</em>, <em>Pseudomonas </em>and <em>Xanthomonas</em>) were streaked on five bacteriological media (NA, King B, K, R2A and 523) and on the medium used for plant culture initiation – ½ MS with milk albumin (IM). All strains grew on all media but on K and IM at the slowest rate and on 523 medium at the fastest. The IM medium proved to be useful for immediate bacteria detection at the initial stage of culture. In the second experiment, aiming at characterization of isolates on the basis of colony growth and morphology 14 strains (<em>Agrobacterium</em>, <em>Bacillus</em>, <em>Curtobacterium, Flavobacterium</em>, <em>Lactobacillus</em>, <em>Methylobacterium </em>– 2 strains <em>Mycobacterium</em>, <em>Paenibacillus</em>, <em>Plantibacterium</em>, <em>Pseudomonas, Stenotrophomonas</em>, <em>Xanthomonas, </em>and species <em>Serratia marcescens</em>) were streaked on five microbiological media: KB, NBY, YDC, YNA and YPGA<em>. </em>All strains grew on all those media but at different rates. The only exception was the strain of <em>Lactobacillus </em>spp., which did not grow on King B medium. This medium allowed the detection of such characteristic traits as fluorescence (<em>Pseudomonas</em>) and secretion of inclusions (<em>Stenotrophomonas</em>). The third experiment was focussed on assessment of the sensitivity of detection of specific bacteria in pure cultures and in plant tis- sue cultures using standard PCR and BIO-PCR techniques with genus specific primers and 2 methods of DNA isolation. Results showed that the use of Genomic Mini kit enabled an increase of the sensitivity by 100 times as compared to extraction of DNA by boiling. Moreover, the application of BIO-PCR increased sensitivity of detection from 102 to 105 times over the standard PCR. If looking for unknown cultivable bacteria more effective detection seems to be use of microbiological method enabling detection on bacteriological media single cells in the fragments of explants or in wash liquids, in which fragmented explants were shaken.
24
Zeldin, Eric L., David D. Ellis, and Brent H. McCown. "STABLE TAXANE PRODUCTION IN TAXUS SHOOT CULTURES." HortScience 27, no. 6 (June 1992): 585b—585. http://dx.doi.org/10.21273/hortsci.27.6.585b.
Повний текст джерелаАнотація:
Taxol, a promising anticancer drug, is limited by inadequate supply. The production of taxol and related compounds (taxanes) by Taxus tissue cultures has been reported, yet sustained production has not been demonstrated. One theory is that cell differentiation and/or tissue organization is required to sequester taxol and avoid autotoxicity. To investigate this, T. cuspidata shoot cultures were established and the taxane content of various culture stages compared to that of field needles. HPLC analysis identified two peaks which comigrated and had UV spectra identical to taxol and 10-deacetyl taxol. The levels of 10-deacetyl taxol were similar in all samples. Cultured shoots contained much less taxol than field needles, and the level of a third peak which migrates closely to taxol was inversely related to that of taxol. Taxol content was restored in the first flush out of culture. Shoot cultures of T. brevifolia, T. x media, and T. canadensis have also been analyzed. In addition to shoot cultures, nodule cultures, a biological unit that may be suitable for production of taxanes in plant bioreactors, have been initiated and characterized.
25
Goldhaber, P., and L. Rabadjija. "H+ stimulation of cell-mediated bone resorption in tissue culture." American Journal of Physiology-Endocrinology and Metabolism 253, no. 1 (July 1, 1987): E90—E98. http://dx.doi.org/10.1152/ajpendo.1987.253.1.e90.
Повний текст джерелаАнотація:
The addition of protons in the form of hydrochloric acid (10.5, 17.2, or 26.6 meq/l resulting in an initial media pH of 7.28, 7.15, and 6.94, respectively) to neonatal mouse calvaria maintained in a chemically defined medium in tissue culture for 1 wk increased calcium release in a dose-response fashion. The same amounts of protons added to the media of devitalized calvaria caused no increase in calcium release into the medium. The net cell-mediated calcium release resulting from the addition of 26.6 meq/l of protons amounted to approximately 50% of the initial calvarial calcium content. Hydroxyproline determinations revealed that active resorption was taking place, wherein both mineral and organic matrix are removed simultaneously. Histological examination of the extensively resorbed calvaria demonstrated the presence of numerous osteoclasts in different stages of bone destruction. The addition of indomethacin (100 ng/ml) strongly inhibited the increase in calcium release by added protons, suggesting that prostaglandin synthesis is involved in the phenomenon. The addition of thyrocalcitonin also inhibited proton-induced calcium release, providing additional evidence that the calcium release from cultures exposed to added protons involved osteoclastic activity.
26
Berg, Lisa C., and Henry R. Owen. "A Comparison between Synthetic and Organic Iron Chelators on in Vitro Growth of Nicotiana tabacum Callus Cultures." HortScience 31, no. 4 (August 1996): 630g—631. http://dx.doi.org/10.21273/hortsci.31.4.630g.
Повний текст джерелаАнотація:
Nicotiana tabacum callus growth (fresh weight) was measured after culture in the light (16-hour photoperiod) or in darkness for four different culture media, differing in iron chelate type or concentration. All media contained MS basal medium supplemented with 30 g·L–1 sucrose, 2 mg·L–1 IAA, 0.2 mg·L–1 KIN, and 7 g·L–1 agar, pH 5.8. Three of the media contained iron-metalosate (Albion Laboratories), an organic iron chelate, at 100, 200, and 400 micromolar concentrations, and the fourth medium contained 100 μm Fe-EDTA. Twenty-five culture tubes were prepared for each of the 4 different media concentrations and 2 light treatments (8 treatments total). A 1-cm3 callus explant was used for each treatment and cultured for 56 days at 20°C. About 20-fold increases in callus fresh weight were observed for cultures incubated in light or in darkness. In addition, callus growth was not significantly affected by iron chelate type, suggesting the potential utility of this organic chelator in tissue culture media to alleviate potential problems of light-induced EDTA instability and subsequent IAA inactivation. These cultures are being maintained to examine the influence of iron chelate type on organogenesis.
27
Nicomrat, Duongruitai, and Jackrit Anantasaran. "A Reliable Homemade Tissue Culture Protocol for Dendrobium Orchid Cultivation." Applied Mechanics and Materials 804 (October 2015): 227–30. http://dx.doi.org/10.4028/www.scientific.net/amm.804.227.
Повний текст джерелаАнотація:
Murashige and Skoog (MS) medium, a common tissue culture media for orchids which is mostly supplemented with various natural organic substances with high nutritional values can accelerate the growth of plant tissues. Nevertheless, the knowledge of actual compositions of these natural added substances is limited, causing various culture results. In this study, we have investigated the effects of added natural organic nutrient ratios on 2 orchid tissue cultures, Dendrobium, Dendrobium farmeri Paxt. and Dendrobium griffithianum Lindl. Additionally, simple, affordable medium recipes, MS media supplemented with banana were thus economically sterilized by either a simple type steaming vapor boiler or an autoclave for reliability improvement. The results showed that the orchids grew better in medium supplemented with Namwa banana especially at 150 g/L for Dendrobiumfarmeri Paxt. and 75 g/L for Dendrobium griffithianum Lindl. The suitable steaming procedure at 100°C for 60 minutes was adequate for eliminating most pathogens from the media and helped both orchid seedlings cultivated very well. Moreover, the optimized homemade tissue culture protocol provides fast effectiveness, simplicity, consistently high nutritional values, and practically being suitable technique for ready transferring to the community.
28
Ribeiro, Gesiane, Cristina O. Massoco, and José Corrêa de Lacerda Neto. "Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media." Pesquisa Veterinária Brasileira 33, suppl 1 (December 2013): 20–24. http://dx.doi.org/10.1590/s0100-736x2013001300004.
Повний текст джерелаАнотація:
The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.
29
Uhlmann, Erik J., Rosalia Rabinovsky, Hemant Varma, Rachid El Fatimy, Ekkehard M. Kasper, Justin M. Moore, Rafael A. Vega, et al. "Tumor-Derived Cell Culture Model for the Investigation of Meningioma Biology." Journal of Neuropathology & Experimental Neurology 80, no. 12 (November 28, 2021): 1117–24. http://dx.doi.org/10.1093/jnen/nlab111.
Повний текст джерелаАнотація:
Abstract Meningioma is the most common primary central nervous system tumor. Although mostly nonmalignant, meningioma can cause serious complications by mass effect and vasogenic edema. While surgery and radiation improve outcomes, not all cases can be treated due to eloquent location. Presently no medical treatment is available to slow meningioma growth owing to incomplete understanding of the underlying pathology, which in turn is due to the lack of high-fidelity tissue culture and animal models. We propose a simple and rapid method for the establishment of meningioma tumor-derived primary cultures. These cells can be maintained in culture for a limited time in serum-free media as spheres and form adherent cultures in the presence of 4% fetal calf serum. Many of the tissue samples show expression of the lineage marker PDG2S, which is typically retained in matched cultured cells, suggesting the presence of cells of arachnoid origin. Furthermore, nonarachnoid cells including vascular endothelial cells are also present in the cultures in addition to arachnoid cells, potentially providing a more accurate tumor cell microenvironment, and thus making the model more relevant for meningioma research and high-throughput drug screening.
30
Senanayake, P. deS, A. Calabro, K. Nishiyama, J. G. Hu, D. Bok, and J. G. Hollyfield. "Glycosaminoglycan synthesis and secretion by the retinal pigment epithelium: polarized delivery of hyaluronan from the apical surface." Journal of Cell Science 114, no. 1 (January 1, 2001): 199–205. http://dx.doi.org/10.1242/jcs.114.1.199.
Повний текст джерелаАнотація:
Hyaluronan and chondroitin sulfate glycosaminoglycan secretion from retinal pigment epithelial cells was established in confluent cultures with high transepithelial resistance. Cell cultures were maintained on Millicell-PCF culture plates, which allow separation of culture medium exposed to apical and basal epithelial surfaces. Following various times in culture, apical and basal culture media were sampled at three day intervals and the glycosaminoglycan content was quantified. Samples were digested with proteinase K to free the glycosaminoglycans from their core proteins, the glycosaminoglycans were ethanol precipitated, and subjected to hyaluronidase SD and chondroitinase ABC digestion to release hyaluronan and chondroitin sulfate disaccharides. Disaccharides were fluorotagged with 2-aminoacridone, separated on polyacrylamide gels and the molar fluorescence in each disaccharide band quantitated. Hyaluronan in the apical medium was significantly higher than in the basal medium (5-12 times) at all recovery intervals (P<0.0001). In contrast, the distribution of unsulfated chondroitin, 4-sulfated chondroitin and 6-sulfated chondroitin disaccharides in apical and basal media was non-polar. Confocal microscopy of cultures probed with a hyaluronan-specific fluorotag established that the HA evident in these cultures is restricted to the apical border of the RPE cultures. Collectively, these data indicate that hyaluronan synthesized by the retinal pigment epithelium is secreted preferentially from the apical surface, suggesting that this tissue is an important source of hyaluronan present in the interphotoreceptor matrix.
31
Pampaloni, Francesco, Ulrich Berge, Anastasios Marmaras, Peter Horvath, Ruth Kroschewski, and Ernst H. K. Stelzer. "Tissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions." Integr. Biol. 6, no. 10 (2014): 988–98. http://dx.doi.org/10.1039/c4ib00121d.
Повний текст джерела
32
Bastaki, Shahraban, Mostafa AboEl-Nil, and Mahdi Abdal. "EMBRYOGENESIS IN EGGPLANT COTYLEDON CULTURE." HortScience 27, no. 6 (June 1992): 693f—693. http://dx.doi.org/10.21273/hortsci.27.6.693f.
Повний текст джерелаАнотація:
Eggplant (Solanum melonga L.) cotyledons were used to form somatic embryos for somaclonal induction and for selection of salt tolerant genotypes in a genetic improvement program. Naphthalene acetic acid at concentrations ranged from 5 uM to 85 uM induced embryogenesis when cultures were incubated under 16 hrs of light photoperiod. NAA was the only growth regulator required, and the addition of kinetine and benzyl adenine inhibited embryo formation. High frequency embryogenesis formed in 2 week old cotyledons when cultured on a medium supplemented with 43 uM NAA. Data showed that varieties varied in their embryogenesis potential and that cotyledons were the most responsive tissue. Somatic embryos germinated into plantlets when transferred into media without any growth regulators. Somatic embryos were plated on germination media supplemented with Kuwait brackish water to increase the total dissolved salts in the medium from 4,770 ppm to 30,000 ppm in seven equal increments. Brackish water at all concentrations caused embryos to revert into profuse callus growth.
33
Ab Rahman, Zuraida, Mohd Shukri Mat Ali, Mohd Norfaizal Ghazalli, Khadijah Awang, and Ayu Nazreena Othman. "Optimization of Culture Media Formulations for Micropropagation of Lepisanthes fruticosa." Biosciences, Biotechnology Research Asia 15, no. 1 (March 24, 2018): 51–58. http://dx.doi.org/10.13005/bbra/2607.
Повний текст джерелаАнотація:
Tissue culture provides an avenue for the production of high quality clonal plants in large numbers within a short time. Here, we describe the development of protocols for reproducible in vitro micropropagation of Lepisanthes fruticosa via direct organogenesis. Shoots were initiated from two types of explants, nodes and young shoots, to establish in vitro cultures on Murashige and Skoog’s (MS) medium or Woody Plant Medium (WPM) supplemented with different concentrations of benzylaminopurine (BAP). Semi-solid WPM media containing 1 mg/L BAP was most effective in shoot initiation in both node and young shoot explants, giving 40% and 20% shoot induction, respectively. The highest rate of shoot proliferation from young shoot explants was obtained using BAP at 3.0 mg/L in combination with NAA at 1.0 mg/L in WPM culture medium. This combination of growth regulators in the medium was also suited to root initiation.
34
McMullen, Allison R., Caline Mattar, Nigar Kirmani, and Carey-Ann D. Burnham. "Brown-Pigmented Mycobacterium mageritense as a Cause of Prosthetic Valve Endocarditis and Bloodstream Infection." Journal of Clinical Microbiology 53, no. 8 (June 10, 2015): 2777–80. http://dx.doi.org/10.1128/jcm.01041-15.
Повний текст джерелаАнотація:
Mycobacterium spp. are a rare cause of endocarditis. Herein, we describe a case of Mycobacterium mageritense prosthetic valve endocarditis. This organism produced an unusual brown pigment on solid media. Cultures of valve tissue for acid-fast bacilli might be considered in some cases of apparently culture-negative prosthetic valve endocarditis.
35
Mahdieh, Majid, Mitra Noori, and Simin Hoseinkhani. "Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus." Plant Tissue Culture and Biotechnology 25, no. 1 (July 9, 2015): 63–70. http://dx.doi.org/10.3329/ptcb.v25i1.24126.
Повний текст джерелаАнотація:
Adventitious root culture of leaf explants of R. crispus was established using different MS supplemented with different concentrations of auxins and a combination of NAA and Kn for growth and flavonoids production. Among the different auxins, NAA was more effective than IAA to induce adventitious roots. Adventitious roots grown on MS containing 5 ?M NAA and 0.5 ?M Kn showed the highest root growth, as well as the highest amount of total flavonoids (= 6) as compared with roots grown in other media. Chromatographic purification of the root extract showed that flavonoid composition also was influenced by hormone combinations in the culture media. The addition of Kn to the medium reduced or suppressed myricetin (M) and naringenin (N) production. Quercetin (Q) was not found in media containing Kn alone similar to the control medium. Isorhamnetin (I), kaempferol (K) and rutin (R) were produced in the roots on media supplemented with all hormone combinations, but were absent in 0.1 ?M Kn supplemented media similar to the control roots.Plant Tissue Cult. & Biotech. 25(1): 63-70, 2015 (June)
36
Nagmani, R., A. M. Diner, and G. C. Sharma. "Somatic embryogenesis in longleaf pine (Pinuspalustris)." Canadian Journal of Forest Research 23, no. 5 (May 1, 1993): 873–76. http://dx.doi.org/10.1139/x93-115.
Повний текст джерелаАнотація:
Isolated zygotic embryos and female gametophytes containing zygotic embryos were cultured on MSG and DCR basal media, supplemented with three different carbon sources added individually to the medium at four levels each. The media also contained various levels of 2,4-dichlorophenoxy acetic acid (2,4-D) and N6-benzyladenine (BA). Embryogenic tissue extruded from female gametophytes during 4 weeks in culture on media containing either glucose or maltose or sucrose. Embryogenic tissue initiation was most frequently from explants collected on July 14, 1992, when the zygotic embryos within the female gametophytes were precotyledonary. A total of 33 embryogenic cultures were initiated from 944 explants cultured. One of 192 explants cultured on basal media with no growth regulators produced embryogenic tissue. The embryogenic tissue showed numerous somatic embryos at stages 1 and 2 of development, corresponding to their zygotic embryo counterparts.
37
JADCZAK, Paula, and Danuta KULPA. "Lavandula angustifolia PROPAGATED IN IN VITRO CULTURES ON MEDIA CONTAINING AgNPs AND AuNPs – AN ALTERNATIVE TO SYNTHETIC PRESERVATIVES IN COSMETICS." Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura, Alimentaria, Piscaria et Zootechnica 357, no. 56 (December 6, 2020): 5–18. http://dx.doi.org/10.21005/aapz2020.56.4.01.
Повний текст джерелаАнотація:
We determined the preservation properties of Lavandula angustifolia propagated on media with gold or silver nanoparticles with a particle size of 13 and 30 nm. Cosmetic emulsions prepared by using lavender tissue that was propagated on media containing AuNPs and AgNPs showed increased preservative capacities when compared with the control ones. In the case of control cosmetic emulsions, which had no added plant tissues or dehydroacetic acid and benzoic acid (DHA BA), bacterial and fungal colonies appeared after the second week of the experiment. The addition of lavender tissue propagated on media without AuNPs or AgNPs protected the tasted samples from microbial contamination; in this case, bacterial contamination was detected after 4 weeks and fungal contamination after 6 weeks. The addition of lavender tissue propagated on medium containing AgNPs with a particle size of 13 nm at a concentration of 1 mg · dm−3 prolonged the time of detection of bacteria colonies to 8 weeks (0.9) and this result was close and comparable to the effect of DHA BA. Higher concentrations of AgNPs in the culture medium, as well as a larger particle diameter (30 nm), resulted in the decreased preservative capacity of plant tissues. The presence of AuNPs in the culture media showed a positive effect on the antimicrobial activity of lavender; however, to a lesser degree than in the case of AgNPs. Disintegrated fragments of lavender tissue propagated on media containing 1 mg ∙ dm−3 AgNPs with particle size of 13 nm can be used to preserve short shelf life cosmetic emulsions.
38
Adamczyk-Rogozińska, Urszula, and Halina Wysokińska. "Organ and plantlet regeneration of Menyanthes trifoliata through tissue culture." Acta Societatis Botanicorum Poloniae 67, no. 2 (2014): 161–66. http://dx.doi.org/10.5586/asbp.1998.018.
Повний текст джерелаАнотація:
The conditions for the regeneration of plants through organogenesis from callus tissues of <em>Menyanthes trifoliata</em> are described. The shoot multiplication rate was affected by basal culture media, the type and concentration of cytokinin and subculture number. The best response was obtained when caulogenic calli were cultured on the modified Schenk and Hildebrandt medium (SH-M) containing indole-3-acetic acid (IAA 0,5 mg/l) and 6-benzyladenine (BA 1 mg/l) or zeatin (2 mg/l). Under these conditions ca 7 shoots (mostly 1 cm or more in length) per culture in the 5th and 6th passages could be developed. In older cultures (after 11-12 passages) there was a trend for more numerous but shorter shoot formation. All regenerated shoots could be rooted on the SH-M medium supplemented with 0.5 mg/l IAA within 6 weeks; 80% of in vitro rooted plantlets survived their transfer to soil.
39
Bunge, R. P. "Tissue culture observations relevant to the study of axon-Schwann cell interactions during peripheral nerve development and repair." Journal of Experimental Biology 132, no. 1 (September 1, 1987): 21–34. http://dx.doi.org/10.1242/jeb.132.1.21.
Повний текст джерелаАнотація:
During peripheral nerve development the Schwann cell population is expanded so that adequate numbers are available for ensheathment of both nonmyelinated and myelinated nerve fibres. As ensheathment of these fibres progresses each axon--Schwann cell unit becomes surrounded by a basal lamina, providing a unique microtubular framework within the peripheral nerve trunk. Tissue culture studies of pure populations of neurones and Schwann cells cultured separately and in combination indicate that a surface component on the axon provides a mitogenic signal to Schwann cells requiring cell-cell contact. Biochemical, electron microscopic and immunocytochemical analyses of these cultures indicate that Schwann cells in contact with axons are able to generate a basal lamina (containing type IV collagen, laminin and heparan sulphate proteoglycan) and fibrous collagen, without the aid of other cells, and that axonal contact is required for deposition of the basal lamina. The role of Schwann cells and the extracellular matrix they synthesize and organize, as well as the role of the other known products of the Schwann cells in the process of peripheral nerve regeneration, are discussed. It is suggested that the large numbers and advantageous position of the Schwann cells, as well as their ability to provide their own surfaces, a basal lamina and multiple secretory products, may account for their extraordinary ability to foster nerve fibre regeneration.
40
Andersen, Knut-Jan, Erik Ilsø Christensen, and Hogne Vik. "Three-dimensional Growth of Renal Epithelial Cells in Vitro: A Tool in Toxicity Testing." Alternatives to Laboratory Animals 21, no. 2 (April 1993): 191–95. http://dx.doi.org/10.1177/026119299302100212.
Повний текст джерелаАнотація:
The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.
41
Murín, R., K. Mészáros, P. Nemeček, R. Kuna, and J. Faragó. "Regeneration of immature and mature embryos from diverse sets of wheat genotypes using media containing different auxins." Acta Agronomica Hungarica 60, no. 2 (June 1, 2012): 97–108. http://dx.doi.org/10.1556/aagr.60.2012.2.2.
Повний текст джерелаАнотація:
The effect of explant type (immature vs. mature embryos) and two auxin types (2,4-dichlorophenoxyacetic acid vs. Dicamba) on the callogenesis and plant regeneration ability of 26 wheat cultivars was studied. In general, the callus induction, plant regeneration and shoot formation frequencies were higher in mature embryo-derived cultures as compared to immature ones on media originally developed for mature wheat embryo cultures. In both culture types, the auxin Dicamba was found to be more efficient, especially when mature embryos were cultured. The separation of means using Duncan’s multiple range test revealed the best in vitro response, in terms of the frequency of callus regeneration, in the cultivar Astella for both immature and mature embryo cultures. This cultivar gave very promising results, suggesting that it could be used in the future for further tissue culture investigations and as a donor material for genetic transformation experiments in wheat. Correlation analyses revealed significant similarities between the evaluated parameters within each group (immature and mature embryo-derived cultures). However, there were no significant correlations between these two groups for most of the parameters. This suggests that the mechanism of plant regeneration in the two in vitro regeneration systems (mature vs. immature embryo culture) may be different enough to hamper the development of an optimal plant regeneration protocol for use in both systems.
42
Barton, Jack, Katherine Pacey, Neha Jain, Tessa Kasia, Darren Edwards, Christine Thevanesan, Karin Straathof, Giuseppe Barone, and John Anderson. "Establishment and phenotyping of neurosphere cultures from primary neuroblastoma samples." F1000Research 8 (June 10, 2019): 823. http://dx.doi.org/10.12688/f1000research.18209.1.
Повний текст джерелаАнотація:
Background: Primary cell culture using serum free media supplemented with growth factors has been used in a number of cancers to propagate primary cells with stem like properties, which form as spherical cellular aggregates. Methods: We systematically evaluated the capacity of freshly disaggregated neuroblastoma tumors to become established as neurospheres in stem cell media using a uniform protocol. 67 primary neuroblastoma samples from patients treated at a single institution were prospectively evaluated for their ability to become established in culture. Samples, either solid tissue or cells from surgical transit fluid both post chemotherapy and chemotherapy naïve, were evaluated from diagnostic needle biopsies or surgical resections. Results: Overall 37 neurosphere cultures were successfully established from 67 samples. In 11 out of 14 cases investigated by flow cytometry, uniform staining for neuroblastoma markers CD56 and GD2 was demonstrated in CD45 negative non-hemopoietic cells, confirming neuroblastoma origin. Conclusion: We present a simple and reproducible approach for producing primary neurospheres from neuroblastoma samples, which provides a reliable resource for future work including genetic analysis, stem cell research and models for therapeutics.
43
Taylor, W. R., and R. W. Alexander. "Autocrine control of wound repair by insulin-like growth factor I in cultured endothelial cells." American Journal of Physiology-Cell Physiology 265, no. 3 (September 1, 1993): C801—C805. http://dx.doi.org/10.1152/ajpcell.1993.265.3.c801.
Повний текст джерелаАнотація:
The repair process of the vascular endothelium is modulated by growth factors from both endogenous (within the vessel wall) and exogenous (blood borne) sources. We utilized a tissue culture model of endothelial wounding to gain further insight into the potential autocrine control of proliferation during wound repair. Cultured porcine aortic endothelial monolayers were mechanically wounded by passing a 7-mm sterile glass rod over the surface of the culture. Proliferation at the wound edge was quantified using [3H]thymidine autoradiography. In wounded cultures incubated in media supplemented with 10% fetal calf serum, 81 +/- 2% of the nuclei at the wound edge were labeled. When the cultures were incubated in serum-free media, proliferation at the wound edge was only slightly diminished with 65 +/- 3% (P < 0.05) of the cells labeled. These findings raise the possibility that there is a significant contribution from autocrine growth factors to endothelial wound repair. To evaluate the potential role of insulin-like growth factor I (IGF-I) in the wound repair process, we used a radioimmunoassay to measure IGF-I secretion. Wounded cultures exhibited a 187 +/- 58% increase in IGF-I production when compared with nonwounded cultures (P < 0.05). To determine the extent to which endogenous IGF-I mediates the proliferative response of endothelial cell monolayers to wounding, wounded cultures were incubated with inactivating concentrations of IGF-I antibody. When IGF-I antibody was present in the culture media, only 26 +/- 3% of the nuclei at the wound edge were labeled with [3H]thymidine (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
44
Vonk, Ariel C., Sarah C. Hasel-Kolossa, Gabriela A. Lopez, Megan L. Hudnall, Darian J. Gamble, and Thomas P. Lozito. "Lizard Blastema Organoid Model Recapitulates Regenerated Tail Chondrogenesis." Journal of Developmental Biology 10, no. 1 (February 10, 2022): 12. http://dx.doi.org/10.3390/jdb10010012.
Повний текст джерелаАнотація:
(1) Background: Lizard tail regeneration provides a unique model of blastema-based tissue regeneration for large-scale appendage replacement in amniotes. Green anole lizard (Anolis carolinensis) blastemas contain fibroblastic connective tissue cells (FCTCs), which respond to hedgehog signaling to create cartilage in vivo. However, an in vitro model of the blastema has not previously been achieved in culture. (2) Methods: By testing two adapted tissue dissociation protocols and two optimized media formulations, lizard tail FCTCs were pelleted in vitro and grown in a micromass blastema organoid culture. Pellets were analyzed by histology and in situ hybridization for FCTC and cartilage markers alongside staged original and regenerating lizard tails. (3) Results: Using an optimized serum-free media and a trypsin- and collagenase II-based dissociation protocol, micromass blastema organoids were formed. Organoid cultures expressed FCTC marker CDH11 and produced cartilage in response to hedgehog signaling in vitro, mimicking in vivo blastema and tail regeneration. (4) Conclusions: Lizard tail blastema regeneration can be modeled in vitro using micromass organoid culture, recapitulating in vivo FCTC marker expression patterns and chondrogenic potential.
45
Tripepi, Robert R., Holly J. Schwager, Mary W. George, and Joseph P. McCaffrey. "Eliminating Thrips in Microshoot Cultures." HortScience 33, no. 3 (June 1998): 506d—506. http://dx.doi.org/10.21273/hortsci.33.3.506d.
Повний текст джерелаАнотація:
Two insecticides, acephate or azadirachtin, were added to tissue culture media to determine their effectiveness in controlling onion thrips (Thrips tabaci Lindeman.) and to determine if these insecticides could damage the plant shoot cultures. To test for insecticide phytotoxicity, microshoots from European birch (Betula pendula), American elm (Ulmus americana), `Pink Arola' chrysanthemum (Dendranthema grandiflora), `America' rhododendron (Rhododendron catawbiense), `Golden Emblem' rose (Rosa hybrida), and `Gala' apple (Malus domestica) were placed in 130-ml baby food jars containing 25 ml of medium supplemented with 6.5, 13, or 26 mg/l Orthene® (contained acephate) or 0.55, 1.1, or 2.2 ml/l Azatin® (contained azadirachtin). Control jars lacked insecticide. To test for thrips control, 13 mg/l Orthene® or 0.55 ml/l Azatin® was added to Murashige and Skoog medium, and 10 thrips were placed on `Gala' apple microshoots in each jar. Jars were sealed with plastic wrap. In both studies, microshoot dry weight and heights were determined. In the second study, the total number of thrips per jar was also determined 3 weeks after inoculation. Microshoots on Orthene®-treated media lacked phytotoxicity symptoms, regardless of the concentration used. In contrast, Azatin® hindered plant growth, decreasing shoot height or dry weight by up to 85% depending on the species. Both insecticides prevented thrips populations from increasing, since less than 10 thrips were found in jars with insecticide-treated medium. Control jars, however, contained an average of almost 70 thrips per jar. This study demonstrated that both Orthene® and Azatin® were effective for eradicating thrips from plant tissue cultures, but Orthene® should probably be used because Azatin® was phytotoxic to all species tested.
46
El-Baz, Farouk K., Amal A. Mohamed, and Sami I. Ali. "Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)." Nova Biotechnologica et Chimica 10, no. 2 (August 31, 2021): 79–94. http://dx.doi.org/10.36547/nbc.1118.
Повний текст джерелаАнотація:
Callus cultures from stems, leaves and roots of colocynth were initiated on MS media supplemented with various combinations of 2,4 dichlorophenoxyacetic acid (2,4-D) with kinetin (KIN) and benzyladenine (BA) with α-naphthaleneacetic acid (NAA). The highest percentage of callus formation frequency (98.9%) was obtained from stem explants grown on MS media supplemented with (1.0 mg/L) 2,4-D + (1.0 mg/L) KIN. The total phenolics and flavonoid content of the colocynth callus cultures were measured. The results showed that the MS medium supplemented with 6.0 mg/L 2,4-D + 2.0 mg/L KIN (MD3) gave the highest content of total phenolics (19.2 mg/100g d.w.) in leaf-derived calli. The highest content of flavonoids (47.3 mg/100g d.w.) was obtained in stem derived calli grown on the same medium (MD3). Antioxidant activities of extracts were determined using different assays, including DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power. Leaf-derived calli cultured on MS medium + 2.0 mg/L 2,4-D + 1.0 mg/L KIN (MD1) showed the highest DPPH radical scavenging activity (85.3%). The highest percentage of H2O2 scavenging activity (61.4%) was detected in leaf explant-derived calli growing on MD1. The leaf-derived calli growing on (MD3) gave the highest ferric reducing power (22.3 μg/g d.w.), compared to the activities of stems, leaves and roots of in vitro grown seedlings (3.28, 12.9 and 2.85 μg/g d.w.), which were used as controls. On the basis of the current findings, we conclude that MS media supplemented with different combinations of 2,4-D and KIN yields higher phenolics, flavonoids contents and antioxidant activities than MS media supplemented with BA and NAA.
47
Braithwaite, Kathryn S., Chuong N. Ngo, and Barry J. Croft. "Confirmation that the Novel Cercozoa Phytocercomonas venanatans Is the Cause of the Disease Chlorotic Streak in Sugarcane." Phytopathology® 108, no. 4 (April 2018): 487–94. http://dx.doi.org/10.1094/phyto-07-17-0236-r.
Повний текст джерелаАнотація:
A cercomonad, named Phytocercomonas venanatans, is confirmed as the cause of the sugarcane disease chlorotic streak. This was achieved by establishing aseptic liquid cultures of the pathogen isolated from internal pieces of sugarcane stalk tissue. Actively motile cultures of the pathogen were inoculated into sugarcane roots, stalks, and leaf whorls. Infected plants subsequently developed the characteristic symptoms of chlorotic streak. Infection was confirmed by PCR screening of plant tissues and by reisolation of the pathogen into aseptic culture followed by PCR and microscopic confirmation. P. venanatans is the first reported pathogenic cercomonad able to systemically infect higher plants and the first plant pathogenic cercozoan able to be successfully grown in axenic culture on common microbiological media.
48
Chato-Astrain, Jesús, David Sánchez-Porras, Óscar Darío García-García, Claudia Vairo, María Villar-Vidal, Silvia Villullas, Indalecio Sánchez-Montesinos, Fernando Campos, Ingrid Garzón, and Miguel Alaminos. "Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers." Biomedicines 9, no. 11 (November 6, 2021): 1634. http://dx.doi.org/10.3390/biomedicines9111634.
Повний текст джерелаАнотація:
Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these cultures in less time. In the present study, we evaluated the effect of novel formulations based on EGF-loaded nanostructured lipid carriers (NLC). First, biosafety of NLC containing recombinant human EGF (NLC-rhEGF) was verified in immortalized skin keratinocytes and cornea epithelial cells, and in two epithelial cancer cell lines, by quantifying free DNA released to the culture medium. Then we established primary cell cultures of human skin keratinocytes with basal culture media (BM) and BM supplemented with NLC-rhEGF, liquid EGF (L-rhEGF), or NLC alone (NLC-blank). The results showed that cells isolated by enzymatic digestion and cultured with or without a feeder layer had a similar growth rate regardless of the medium used. However, the explant technique showed higher efficiency when NLC-rhEGF culture medium was used, compared to BM, L-rhEGF, or NLC-blank. Gene expression analysis showed that NLC-rhEGF was able to increase EGFR gene expression, along with that of other genes related to cytokeratins, cell–cell junctions, and keratinocyte maturation and differentiation. In summary, these results support the use of NLC-rhEGF to improve the efficiency of explant-based methods in the efficient generation of human keratinocyte primary cell cultures for tissue engineering use.
49
Siatka, Tomáš. "Production of Anthocyanins in Callus Cultures of Angelica archangelica." Natural Product Communications 13, no. 12 (December 2018): 1934578X1801301. http://dx.doi.org/10.1177/1934578x1801301219.
Повний текст джерелаАнотація:
Anthocyanins have been used as food color additives, but they also possess many properties beneficial to health. Plant tissue culture technology is an attractive alternative for obtaining these valuable natural pigments. In this work, dark-grown anthocyanin producing callus cultures of Angelica archangelica were established. They were cultured on a Murashige and Skoog medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.4 mg/L benzylaminopurine. Anthocyanin contents in cultures were around 2%, i.e. one order of magnitude higher than in the intact plant that contains up to 0.17% anthocyanins. Growth and production characteristics of the culture were determined – fresh and dry biomass as well as anthocyanin levels reached a maximum on day 30. Effects of basal nutrient media on callus proliferation and anthocyanin accumulation were tested. Culture growth (fresh weight) achieved 105%, 102%, 141%, 129%, 54%, and 26%, and anthocyanin contents attained 114%, 41%, 33%, 31%, 25%, and 15% on Linsmaier and Skoog, Gamborg B5, Schenk and Hildebrandt, Woody plant, Nitsch and Nitsch, and Heller medium, respectively, in comparison with that of Murashige and Skoog.
50
Norman, Jennifer Elise, Andrea Gail Marshall, John C. Rutledge, and Sue C. Bodine. "Skeletal Muscle Stromal Cells Derived From MuRF1 Knockout Mice Are Resistant to Adipogenesis." Journal of the Endocrine Society 5, Supplement_1 (May 1, 2021): A57. http://dx.doi.org/10.1210/jendso/bvab048.115.
Повний текст джерелаАнотація:
Abstract Background: Intramuscular adipose tissue has been found to contribute to muscle dysfunction and is associated with a sedentary lifestyle, aging, and glucocorticoid treatment. Muscle ring finger 1 knockout (MuRF1 KO) mice have been shown to be protected from muscle loss following disuse, aging, and glucocorticoid treatment. In this study we used in vitro techniques to determine if MuRF1 KO muscle stromal cells are resistant to adipogenesis. Methods: Stromal cells were isolated from skeletal muscle tissue of MuRF1 KO and wild type mice. These cells were expanded in culture until 70–90% confluent, then differentiated in either a myogenic media formulation (myogenic cultures) or an adipogenic media formulation (adipogenic cultures). Gene expression was analyzed by qRT-PCR, protein content was measured by bicinchoninic acid assay, and triglyceride content was analyzed by colorimetric assay. We also analyzed isolated stromal cells, which had not been expanded in culture, by flow cytometry to identify myogenic satellite cells (SCs) and fibro-adipogenic progenitors (FAPs). Results: Wild type adipogenic cultures had higher expression of Trim63, the gene encoding MuRF1, when compared to wild type myogenic cultures. Adipogenic cultures had higher expression of adipogenic programing and lipid handling genes than myogenic cultures. These adipogenic cultures also had higher triglyceride content than myogenic cultures. The expression of adipogenic programing genes and lipid handling genes were lower in cultures derived from MuRF1 KO mice compared to wild type derived cultures; however, there was no statistically significant difference in the triglyceride content between the two genotypes. Analysis of stromal cell populations by flow cytometry indicated no difference in the FAP:SC ratio between wild type and MuRF1 KO mice. Conclusions: These results indicate that although there are no differences in the ratio of FAPs to SCs in wild type and MuRF1 KO mice, the MuRF1 KO cultures appear to be resistant to adipogenesis. The mechanism behind this resistance to adipogenesis remains to be elucidated.