Добірка наукової літератури з теми "NanoLC FT MS"
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Статті в журналах з теми "NanoLC FT MS"
Umar, Arzu, Theo M. Luider, John A. Foekens, and Ljiljana Paša-Tolić. "NanoLC-FT-ICR MS improves proteome coverage attainable for ∼3000 laser-microdissected breast carcinoma cells." PROTEOMICS 7, no. 2 (January 2007): 323–29. http://dx.doi.org/10.1002/pmic.200600293.
Повний текст джерелаLakshmanan, Rajeswari, Jeremy J. Wolff, Rudy Alvarado, and Joseph A. Loo. "Top-down protein identification of proteasome proteins with nanoLC-FT-ICR-MS employing data-independent fragmentation methods." PROTEOMICS 14, no. 10 (March 26, 2014): 1271–82. http://dx.doi.org/10.1002/pmic.201300339.
Повний текст джерелаBarnes, Stephen, Erin M. Shonsey, Shannon M. Eliuk, David Stella, Kerri Barrett, Om P. Srivastava, Helen Kim, and Matthew B. Renfrow. "High-resolution mass spectrometry analysis of protein oxidations and resultant loss of function." Biochemical Society Transactions 36, no. 5 (September 19, 2008): 1037–44. http://dx.doi.org/10.1042/bst0361037.
Повний текст джерелаChew, H. K., S. Miyamoto, H. An, D. Rocke, and C. Lebrilla. "Serum glycan analysis in metastatic breast cancer." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 11504. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.11504.
Повний текст джерелаLin, Xionghao, Elena Afia Adjei, Namita Kumari, Sharmin Diaz, Marina Jerebtsova, Patricia A. Oneal, and Sergei Nekhai. "Semi-Automatic Enrichment with High Resolution/Selected Reaction Monitoring (HR/SRM) Scan for the Detection of Urinary Hepcidin in Patients with Sickle Cell Disease." Blood 126, no. 23 (December 3, 2015): 3418. http://dx.doi.org/10.1182/blood.v126.23.3418.3418.
Повний текст джерелаДисертації з теми "NanoLC FT MS"
Camperi, Julien. "Développement de méthodes séparatives pour la caractérisation d’une glycoprotéine intacte : application à l’hormone chorionique gonadotrophine humaine." Thesis, Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLET018.
Повний текст джерелаGlycosylation is the most common form of post-translational modifications (PTMs) of human proteins, since more than 70% are glycosylated. It regulates numerous biological properties including their stability, half-life, and activity. Nevertheless, proteins can also exhibit other types of PTMs that lead to a very large number of isoforms, varying in mass, properties and concentration in the biological samples. Therefore, the characterization of a glycoprotein is highly challenging and requires the use of powerful separation techniques and sensitive and informative detection modes.The human chorionic gonadotropin (hCG) is the hormone specific to human pregnancy. It is essential for the development of placenta and fetus. It is based on two heavily glycosylated subunits, hCGα and hCGβ, having 8 glycosylation sites (4 N- and 4 O-glycosylation sites). Some recent studies demonstrated that here is a correlation between the hCG glycosylation state and the fetus implantation. This is why the characterization of the hCG glycoformes is needed.Therefore, new LC/CE-MS methods were developed for the characterisation of hCG at the intact level using two hCG-based drugs having different glycosylation profiles. While the CZE-MS (TQ) method showed its potential for glycosylation fingerprinting, the complementarity of LC-(qTOF) MS methods in RP and HILIC modes allowed the identification of the glycoforms of the hCGα subunit.To limit the identification errors due to the overlapping of isotopic distribution patterns, the profile of each isoform was resolved by FT-ICR MS. For this purpose, a nanoLC separation in RP mode was developed, thus improving the sensitivity of the method by a factor 500 compared to the conventional format. This method allowed the confirmation of the identification of hCGα glycoforms. Then, it was possible to obtain different glycosylation patterns of the hCGβ by promoting its ionization after hCG reduction. Then, a PNGase treatment was carried out to remove the N-glycans in order to obtain the O-glycoprofiles of hCGβ isoforms
Emmanuel, Alexandra. "Mise en place d’une approche protéomique en vue d’une application au diagnostic d’infections parasitaires congénitales." Electronic Thesis or Diss., Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCB137.
Повний текст джерелаOver the past two decades, mass spectrometry (MS) has become an essential tool in the characterization of proteins such as IgG. These antibodies protect the organism against antigens, which they recognize through the variable domains of their heavy and light chains. Polymorphic sequences also exist at the constant domains CH2 and CH3 of the Fc fragment, involving a few amino acids, which thus define 34 IGHG protein alleles of the 4 IgG subclasses. Relationships are established between some of these polymorphisms and the functionality of the antibody response. The characterization of allelic polymorphism of IgG by MS is frequently carried out by the bottom-up strategy. This analytical approach relies on the analysis of proteins digested by conventional endoproteases (such as trypsin) generating proteolytic peptides (~ 30 amino acids). We have therefore used this method combined with molecular biology approaches to study this polymorphism. However, the presence, for the same individual, of several alleles of very strong homology limits the specific characterization of the sequences of Fc. In this context, we developed a middle-down approach to analyze large peptide fragments (~ 211 amino acids). IdeS is an IgG-specific endoprotease that specifically generates two fragments: the Fc/2 and F (ab')2 fragments. These fragments are then studied by nanoLC-MS/MS at very high resolution (140000 at m/z 200). The fragmentation of the precursor ions was carried out in HCD (higher-energy collisional dissociation) mode. The data obtained were processed and searched in the databases and the results were validated manually. Despite the poor performance of the software available to date due to limited automation, our approach shows that it is possible to discriminate several IGHG alleles from different IgGs. In view of the high sequence homology it is important to optimize the sequence coverage because some alleles differ only by one residue over the 211 amino acids sequenced. This proof of concept of the capabilities of a middle-down analysis to identify different Fc/2 could be used in the future as a diagnostic tool for the characterization of Fc /2 polymorphisms in a context of suspicion of congenital parasitic infections
Тези доповідей конференцій з теми "NanoLC FT MS"
Ahmed, Zamzam Mohammed, Abrar Mohammed Salem, Jose Ramon, Liu Pei Wu, and Benjamin Mowad. "First Successful Pilot Testing of Unconventional Reservoir in North Kuwait from Scratch to Productivity." In Abu Dhabi International Petroleum Exhibition & Conference. SPE, 2021. http://dx.doi.org/10.2118/207394-ms.
Повний текст джерелаAhmed, Zamzam Mohammed, Abrar Mohammed Salem, Liu Pei Wu, and Benjamin Mowad. "Achieving Productivity and Clean Inflow from an Unconventional Reservoir in North Kuwait." In SPE International Hydraulic Fracturing Technology Conference & Exhibition. SPE, 2022. http://dx.doi.org/10.2118/205253-ms.
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