Статті в журналах: "Nanofibres of chitosan"

1

Cheng, Tong, Rolf-Dieter Hund, Dilibaier Aibibu, Jana Horakova, and Chokri Cherif. "Pure Chitosan and Chitsoan/Chitosan Lactate Blended Nanofibres made by Single Step Electrospinning." Autex Research Journal 13, no. 4 (December 31, 2013): 128–33. http://dx.doi.org/10.2478/v10304-012-0040-6.

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Abstract A single step electrospinning of chitosan and chitosan derivative-chitosan lactate nanofibres was studied in this paper. Chitosan was dissolved into acetic acid to produce structure-stable nanofibres. The effect of chitosan concentration and the content of acetic acid on the fibre diameter and morphology of nanofibres were studied in detail. The dynamic viscosity and surface tension of the electrospinning chitosan solutions were systematically studied as well. Based on the fundamental study on electrospinning chitosan in acetic acid, a chitosan derivative, chitosan lactate, was added to produce nanofibre in a pH-friendly aqueous environment. Chemical and morphological analyses demonstrated that chitosan lactate will positively influence the formation of nanofibres in higher pH condition although the morphology should be improved.

2

Che, Ai-Fu, Xiao-Jun Huang, Zhen-Gang Wang, and Zhi-Kang Xu. "Preparation and Surface Modification of Poly(acrylonitrile-co-acrylic acid) Electrospun Nanofibrous Membranes." Australian Journal of Chemistry 61, no. 6 (2008): 446. http://dx.doi.org/10.1071/ch07226.

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Poly(acrylonitrile-co-acrylic acid) (PANCAA) was synthesized and fabricated into nanofibrous membranes by an electrospinning technique. Scanning electron microscopy revealed that membranes composed of uniformly thin and smooth nanofibres were obtained under optimized processing parameters. Surface modification with chitosan on these nanofibrous membranes was accomplished by a coupling reaction between the carboxylic groups of PANCAA and the primary amino groups of chitosan. Fluorescent labelling, weight measurement, FT-IR/ATR spectroscopy, and X-ray photoelectron spectroscopy (XPS) were used to confirm the modification process and determine the immobilization degree of chitosan. Platelet adhesion experiments were further carried out to evaluate the hemocompatibility of the studied nanofibrous membranes. Preliminary results indicated that the immobilization of chitosan on the PANCAA nanofibrous membranes was favourable for platelet adhesion.

3

Korniienko, Viktoriia, Yevheniia Husak, Anna Yanovska, Şahin Altundal, Kateryna Diedkova, Yevhen Samokhin, Yuliia Varava, Viktoriia Holubnycha, Roman Viter, and Maksym Pogorielov. "BIOLOGICAL BEHAVIOUR OF CHITOSAN ELECTROSPUN NANOFIBROUS MEMBRANES AFTER DIFFERENT NEUTRALISATION METHODS." Progress on Chemistry and Application of Chitin and its Derivatives 27 (September 30, 2022): 135–53. http://dx.doi.org/10.15259/pcacd.27.010.

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Chitosan electrospun nanofibres were synthesised in two different trifluoroacetic acid (TFA)/dichloromethane (DCM) solvent ratios and then neutralised in aqueous and ethanol sodium-based solutions (NaOH and Na2CO3) to produce insoluble materials with enhanced biological properties for regenerative and tissue engineering applications. Structural, electronic, and optical properties and the swelling capacity of the prepared nanofibre membrane were studied by scanning electron microscopy, Fourier-transform infrared spectroscopy, and photoluminescence. Cell viability (with the U2OS cell line) and antibacterial properties (against Staphylococcus aureus and Escherichia coli) assays were used to assess the biomedical potential of the neutralised chitosan nanofibrous membranes. A 7:3 TFA/DCM ratio allows for an elaborate nanofibrous membrane with a more uniform fibre size distribution. Neutralisation in aqueous NaOH only maintains a partial fibrous structure. At the same time, neutralisation in NaOH ethanol-water maintains the structure during 1 month of degradation in phosphate-buffered saline and distilled water. All membranes demonstrate high biocompatibility, but neutralisation in ethanol solutions affects cell proliferation on materials made with 9:1 TFA/DCM. The prepared nanofibrous mats could constrain the growth of both gram-positive and gram-negative microorganisms, but 7:3 TFA/DCM membranes inhibited bacterial growth more efficiently. Based on structural, degradation, and biological properties, 7:3 TFA/DCM chitosan nanofibrous membranes neutralised by 70% ethanol/30% aqueous NaOH exhibit potential for biomedical and tissue engineering applications.

4

Hild, Martin, Mohammed Fayez Al Rez, Dilbar Aibibu, Georgios Toskas, Tong Cheng, Ezzedine Laourine, and Chokri Cherif. "Pcl/Chitosan Blended Nanofibrous Tubes Made by Dual Syringe Electrospinning." Autex Research Journal 15, no. 1 (March 1, 2015): 54–59. http://dx.doi.org/10.1515/aut-2015-0016.

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Abstract 3D tubular scaffolds made from Poly-(Ɛ-caprolactone) (PCL)/chitosan (CS) nanofibres are very promising candidate as vascular grafts in the field of tissue engineering. In this work, the fabrication of PCL/CS-blended nanofibrous tubes with small diameters by electrospinning from separate PCL and CS solutions is studied. The influence of different CS solutions (CS/polyethylene glycol (PEO)/glacial acetic acid (AcOH), CS/trifluoroacetic acid (TFA), CS/ AcOH) on fibre formation and producibility of nanofibrous tubes is investigated. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) is used to verify the presence of CS in the blended samples. Tensile testing and pore size measurements are done to underline the good prerequisites of the fabricated blended PCL/ CS nanofibrous tubes as potential scaffolds for vascular grafts. Tubes fabricated from the combination of PCL and CS dissolved in AcOH possesses properties, which are favourable for future cell culture studies.

5

Nthunya, Lebea N., Monaheng L. Masheane, Soraya P. Malinga, Tobias G. Barnard, Edward N. Nxumalo, Bhekie B. Mamba, and Sabelo D. Mhlanga. "UV-assisted reduction of in situ electrospun antibacterial chitosan-based nanofibres for removal of bacteria from water." RSC Advances 6, no. 98 (2016): 95936–43. http://dx.doi.org/10.1039/c6ra19472a.

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6

Jacobs, Valencia, Asis Patanaik, Rajesh D. Anandjiwala, and Malik Maaza. "Optimization of Electrospinning Parameters for Chitosan Nanofibres." Current Nanoscience 7, no. 3 (June 1, 2011): 396–401. http://dx.doi.org/10.2174/157341311795542570.

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7

PROKOPCHUK, N. R., ZH S. SHASHOK, D. V. PRISHСHEPENK, and V. D. MELAMED. "NANOFIBRES ELECTROSPINNING FROM CHITOSAN SOLUTIONS (A REVIEW)." Polymer materials and technologies 1, no. 2 (2015): 36–56. http://dx.doi.org/10.32864/polymmattech-2015-1-2-36-56.

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8

Salehi, Majid, Saeed Farzamfar, Arian Ehterami, Zahrasadat Paknejad, Farshid Bastami, Sadegh Shirian, Hamid Vahedi, Gholamreza Savari Koehkonan, and Arash Goodarzi. "Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies." Journal of Wound Care 29, no. 5 (May 2, 2020): 270–80. http://dx.doi.org/10.12968/jowc.2020.29.5.270.

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Objective: To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds. Method: In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing. To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method. Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line. Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings. Results: The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively. The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres. Conclusion: Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.

9

Abdelgawad, Abdelrahman M., Mehrez E. El-Naggar, Samuel M. Hudson, and Orlando J. Rojas. "Fabrication and characterization of bactericidal thiol-chitosan and chitosan iodoacetamide nanofibres." International Journal of Biological Macromolecules 94 (January 2017): 96–105. http://dx.doi.org/10.1016/j.ijbiomac.2016.07.061.

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10

Fras Zemljič, Lidija, Uroš Maver, Tjaša Kraševac Glaser, Urban Bren, Maša Knez Hrnčič, Gabrijela Petek, and Zdenka Peršin. "Electrospun Composite Nanofibrous Materials Based on (Poly)-Phenol-Polysaccharide Formulations for Potential Wound Treatment." Materials 13, no. 11 (June 9, 2020): 2631. http://dx.doi.org/10.3390/ma13112631.

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In this paper, we focus on the preparation of electrospun composite nanofibrous materials based on (poly)-phenol-polysaccharide formulation. The prepared composite nanofibres are ideally suited as a controlled drug delivery system, especially for local treatment of different wounds, owing to their high surface and volume porosity and small fibre diameter. To evaluate the formulations, catechin and resveratrol were used as antioxidants. Both substances were embedded into chitosan particles, and further subjected to electrospinning. Formulations were characterized by determination of the particle size, encapsulation efficiency, as well as antioxidant and antimicrobial properties. The electrospinning process was optimised through fine-tuning of the electrospinning solution and the electrospinning parameters. Scanning electron microscopy was used to evaluate the (nano)fibrous structure, while the successful incorporation of bio substances was assessed by X-ray Photoelectron Spectroscopy and Fourier transform infrared spectroscopy. The bioactive properties of the formed nanofibre -mats were evaluated by measuring the antioxidative efficiency and antimicrobial properties, followed by in vitro substance release tests. The prepared materials are bioactive, have antimicrobial and antioxidative properties and at the same time allow the release of the incorporated substances, which assures a promising use in medical applications, especially in wound care.

11

Khawar, Muhammd Tauseef, Hugh Gong, Qasim Zia, Hafiza Hifza Nawaz, and Jiashen Li. "Chitosan nanofibres and polypropylene meltblown substrate based multilayer respiratory filter for byssinosis prevention." Journal of Industrial Textiles 52 (August 2022): 152808372211111. http://dx.doi.org/10.1177/15280837221111173.

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Byssinosis is a chronic obstructive pulmonary disease very common in textile cotton workers due to inhalation of fine cotton dust and gram negative bacteria. A three layer composite respiratory filter was developed for Byssinosis prevention by using the combination of polypropylene (PP) based meltblown layers and chitosan nanofibres (CSNF). Filtration efficiency against fine particulates ranges from 100 nm to 2.5 μm and anti-bacterial activity against Pantoea agglomerans were investigated. Chitosan nanofibres were produced by using the electrospinning process and were sandwiched between two PP layers to improve the overall surface area of the filter as well as providing a protective barrier against bacterial pathogens. The filter sample with just 2 hour’s CSNF coating showed more than 99% filtration efficiency with a low pressure drop of 71.6 Pa and a high quality factor value 0.082. The antibacterial performance of CSNF layers achieved up to 91% against the Pantoea agglomerans. Finally, results concluded that the developed respiratory filter can potentially reduce Byssinosis in textile workers.

12

G, Manikandan, Yuvashree M, Sangeetha A, Bhuvana K P, and Sanjay K. Nayak. "Liver Tissue Regeneration using Nano Silver impregnated Sodium Alginate/PVA Composite Nanofibres." SciMedicine Journal 2, no. 1 (March 1, 2020): 16–21. http://dx.doi.org/10.28991/scimedj-2020-0201-3.

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Liver regeneration is a highly organized tissue regrowth process and is the most important reaction of the injured liver. The present study endeavors towards the preparation and characterization of nanoporous Sodium Alginate (SA)/ Poly Vinyl Alcohol (PVA) composite, nanofibrous scaffolds coated with silver (Ag) nanoparticles for hepatocellular regeneration. Chitosan based Silver nanoparticles possess high antibacterial activity has been preferred in the scaffold preparation to improve the antibacterial properties. The structural characterization of Ag Nanoparticles revealed the amorphous nature with an average particle size of 300 nm. Nanofibres (Scaffolds) were prepared by electrospinning SA/PVA solution at a voltage of 18-25 kV and Ag NPs were coated on it for antibacterial activity. Invitro studies denoted the growth of nitro compounds, amides and collagen which are the major constituents of liver tissue.

13

Olvera Bernal, Rigel Antonio, Roman Olegovich Olekhnovich, and Mayya Valerievna Uspenskaya. "Chitosan/PVA Nanofibers as Potential Material for the Development of Soft Actuators." Polymers 15, no. 9 (April 25, 2023): 2037. http://dx.doi.org/10.3390/polym15092037.

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Chitosan/PVA nanofibrous electroresponsive soft actuators were successfully obtained using an electrospinning process, which showed fast speed displacement under an acidic environment. Chitosan/PVA nanofibers were prepared and characterized, and their electroactive response was tested. Chitosan/PVA nanofibers were electrospun from a chitosan/PVA solution at different chitosan contents (2.5, 3, 3.5, and 4 wt.%). Nanofibers samples were characterized using Fourier transform infrared analyses, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), optical microscopy, and tensile test. The electroactive behavior of the nanofiber hydrogels was tested under different HCl pH (2–6) under a constant voltage (10 V). The electroactive response test showed a dependence between the nanofiber’s chitosan content and pH with the bending speed displacement, reaching a maximum speed displacement of 1.86 mm−1 in a pH 3 sample with a chitosan content of 4 wt.%. The results of the electroactive response were further supported by the determination of the proportion of free amine groups, though deconvoluting the FTIR spectra in the range of 3000–3700 cm−1. Deconvolution results showed that the proportion of free amine increased as the chitosan content was higher, being 3.6% and 4.59% for nanofibers with chitosan content of 2.5 and 4 wt.%, respectively.

14

Somsap, Jaruayporn, Kobsak Kanjanapongkul, and Racha Tepsorn. "Effect of parameters on the morphology and fibre diameters of edible electrospun chitosan-cellulose acetate-gelatin hybrid nanofibres." MATEC Web of Conferences 192 (2018): 03038. http://dx.doi.org/10.1051/matecconf/201819203038.

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Electrospinning is the favorite process to fabricate fibres with diameter in the range nanoscale through the action of electric field. In this study, 3-7% chitosan, 18.0% cellulose acetate and 30.0% gelatin solution in aqueous 80% acetic acid solution were blended at the volume ratio of 4:1:5 have been successfully electrospun. The effect of processing parameters and the concentration of the polymer solution on the morphology and diameter of electrospun were investigated. The morphology and diameter of electrospun fibres were observed by scanning electron microscope. The diameters of chitosan-cellulose acetate-gelatin nanofibres ranging from 78.94 to 421.05 nm. The results showed that the fibre diameters increased when the solution concentration and flow rate were increased, whereas the fibre diameters decreased when the applied voltage and distance between tip to collector were increased. The conditions of the solution concentration 18.8 %wt, applied voltage at 23 kV, flow rate at 11.67 μL/min and collector distance at 10 cm were selected to prepares the desirable electrospun nanofibres for applications and the further research.

15

Zeng, R., M. Tu, H. W. Liu, J. H. Zhao, Z. G. Zha, and C. R. Zhou. "Preparation, structure and drug release behaviour of chitosan-based nanofibres." IET Nanobiotechnology 3, no. 1 (2009): 8. http://dx.doi.org/10.1049/iet-nbt:20080010.

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16

Strnad, Simona, and Lidija Fras Zemljič. "Cellulose–Chitosan Functional Biocomposites." Polymers 15, no. 2 (January 13, 2023): 425. http://dx.doi.org/10.3390/polym15020425.

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Here, we present a detailed review of recent research and achievements in the field of combining two extremely important polysaccharides; namely, cellulose and chitosan. The most important properties of the two polysaccharides are outlined, giving rise to the interest in their combination. We present various structures and forms of composite materials that have been developed recently. Thus, aerogels, hydrogels, films, foams, membranes, fibres, and nanofibres are discussed, alongside the main techniques for their fabrication, such as coextrusion, co-casting, electrospinning, coating, and adsorption. It is shown that the combination of bacterial cellulose with chitosan has recently gained increasing attention. This is particularly attractive, because both are representative of a biopolymer that is biodegradable and friendly to humans and the environment. The rising standard of living and growing environmental awareness are the driving forces for the development of these materials. In this review, we have shown that the field of combining these two extraordinary polysaccharides is an inexhaustible source of ideas and opportunities for the development of advanced functional materials.

17

Zhou, Yansheng, Ying Li, Daqing Li, Yidan Yin, and Fenglei Zhou. "Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions." Journal of Functional Biomaterials 13, no. 2 (May 13, 2022): 58. http://dx.doi.org/10.3390/jfb13020058.

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Peripheral nerve injury (PNI) is a neurological disorder that causes more than 9 million patients to suffer from dysfunction of moving and sensing. Using biodegradable polymers to fabricate an artificial nerve conduit that replicates the environment of the extracellular matrix and guides neuron regeneration through the damaged sites has been researched for decades and has led to promising but primarily pre-clinical outcomes. However, few peripheral nerve conduits (PNCs) have been constructed from controllable biodegradable polymeric materials that can maintain their structural integrity or completely degrade during and after nerve regeneration respectively. In this work, a novel PNC candidate material was developed via the electrospinning of polyhydroxy butyrate/chitosan (PHB/CS) composite polymers. An SEM characterisation revealed the resultant PHB/CS nanofibres with 0, 1 and 2 wt/v% CS had less and smaller beads than the nanofibres at 3 wt/v% CS. The water contact angle (WCA) measurement demonstrated that the wettability of PHB/CS electrospun fibres was significantly improved by additional CS. Furthermore, both the thermogravimetric analysis (TGA) and differentiation scanning calorimetry (DSC) results showed that PHB/CS polymers can be blended in a single phase with a trifluoracetic solvent in all compositions. Besides, the reduction in the degradation temperature (from 286.9 to 229.9 °C) and crystallinity (from 81.0% to 52.1%) with increasing contents of CS were further proven. Moreover, we found that the degradability of the PHB/CS nanofibres subjected to different pH values rated in the order of acidic > alkaline > phosphate buffer solution (PBS). Based on these findings, it can be concluded that PHB/CS electrospun fibres with variable blending ratios may be used for designing PNCs with controlled biodegradability.

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Ahmed, Adham, Rob Clowes, Elizabeth Willneff, Peter Myers, and Haifei Zhang. "Porous silica spheres in macroporous structures and on nanofibres." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 368, no. 1927 (September 28, 2010): 4351–70. http://dx.doi.org/10.1098/rsta.2010.0136.

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Porous nanospheres have a wide range of applications such as in catalysis, separation and controlled delivery. Among these nanospheres, syntheses and applications of porous silica nanospheres have been investigated extensively. Uniform porous silica nanospheres can be synthesized using a modified Stöber method. In the present study, porous silica spheres were prepared in the pre-formed emulsion-templated porous polyacrylamide (PAM). A hierarchical hybrid structure of mesoporous silica spheres was formed in the highly interconnected macroporous polymer. The polymer scaffold could be removed by calcination with porous silica spheres and the macroporous structures retained. This resulted from the close packing or aggregation of small silica nanospheres in the pores and on the surface of pores of PAM. The modified Stöber synthesis was further carried out in pre-formed polymer nanofibres (chitosan and sodium carboxymethyl cellulose). The structure of porous silica spheres on nanofibres was produced in the presence of the polymer or composite fibres. The corresponding inorganic structures were successfully obtained after calcination. The hierarchical structures of porous nanospheres within macroporous structures or on nanofibres are of potential interest to researchers in nanomaterials, porous polymers, supported catalysis and controlled delivery.

19

Talebian, S., A. M. Afifi, and H. M. Khanlou. "Fabrication and characterisation of chitosan/poly vinyl alcohol nanofibres via electrospinning." Materials Research Innovations 18, sup6 (December 5, 2014): S6–331—S6–335. http://dx.doi.org/10.1179/1432891714z.000000000979.

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20

Zia, Qasim, Madeeha Tabassum, Zihan Lu, Muhammad Tauseef Khawar, Jun Song, Hugh Gong, Jinmin Meng, Zhi Li, and Jiashen Li. "Porous poly(L–lactic acid)/chitosan nanofibres for copper ion adsorption." Carbohydrate Polymers 227 (January 2020): 115343. http://dx.doi.org/10.1016/j.carbpol.2019.115343.

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21

Nthunya, Lebea N., Monaheng L. Masheane, Soraya P. Malinga, Edward N. Nxumalo, Sabelo D. Mhlanga, and Vijay Kumar Thakur. "Environmentally benign chitosan-based nanofibres for potential use in water treatment." Cogent Chemistry 3, no. 1 (January 1, 2017): 1357865. http://dx.doi.org/10.1080/23312009.2017.1357865.

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22

Steyaert, I., L. Van der Schueren, H. Rahier, and K. de Clerck. "An Alternative Solvent System for Blend Electrospinning of Polycaprolactone/Chitosan Nanofibres." Macromolecular Symposia 321-322, no. 1 (December 2012): 71–75. http://dx.doi.org/10.1002/masy.201251111.

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23

Zinov'ev, Evgeniy V., Marat S. Asadulaev, Denis V. Kostyakov, Kamil F. Osmanov, Roman G. Stoyanovskiy, Natal'ya V. Smirnova, Anton S. Shabunin, and Sergey A. Luk'yanov. "THE POSSIBILITY OF USING THE POLYMER FOR THE TREATMENT OF FULL NANOBIOCOMPOSITES SKIN DEFECTS." Bulletin of the Russian Military Medical Academy 19, no. 1 (December 15, 2017): 46–50. http://dx.doi.org/10.17816/brmma12164.

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The results of the experimental evaluation of the effectiveness of wound dressings based on chitosan nanofibres and copolyamide with treatment of full mechanical skin wounds. To develop their own original technique of mechanical reproduction all layers of the skin wounds in the experiment. It was found that application of wound dressings based on aliphatic copolyamide and chitosan can significantly optimize reparative regeneration processes in the area all layers of the skin defect, thus reducing the time stated scab rejection and wound healing, respectively, 7.5 and 11.2 days (p 0,05). It is shown that the use of coatings based on hyaluronic acid hydrogel is also accompanied by a significant acceleration in the zone histogenesis all layers of the blemishes, shortening the healing period of the final 21% (p 0,05) (4 figs, 1 table, bibliography: 14 refs).

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Adachi, Yu, Takeshi Yabutsuka, and Shigeomi Takai. "Impartation of apatite-forming ability to chitosan nanofibres by using apatite nuclei." IET Nanobiotechnology 14, no. 8 (October 1, 2020): 668–72. http://dx.doi.org/10.1049/iet-nbt.2020.0052.

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25

Jalvandi, Javid, Max White, Yuan Gao, Yen Bach Truong, Rajiv Padhye, and Ilias Louis Kyratzis. "Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release." Materials Science and Engineering: C 73 (April 2017): 440–46. http://dx.doi.org/10.1016/j.msec.2016.12.112.

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26

Fard, Ghazaleh Chizari, Mazeyar Parvinzadeh Gashti, Seyed Ahmad Dehdast, Mohammad Shabani, Ehsan Zarinabadi, Negin Seifi, and Ali Berenjian. "Novel Polyamide/Chitosan Nanofibers Containing Glucose Oxidase and Rosemary Extract: Fabrication and Antimicrobial Functionality." Coatings 14, no. 4 (March 29, 2024): 411. http://dx.doi.org/10.3390/coatings14040411.

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In recent years, the synthesis of nanofibers using plant extracts and bioactive materials has been extensively studied and recognized as a suitable and efficient method applicable in the food packaging field. In this research, an antimicrobial material was introduced by the immobilization of glucose oxidase (GOx) in Nylon–Ag masterbatch/chitosan/Rosmarinus officinalis extract nanofiber via electrospinning technology. Nylon–Ag masterbatch/chitosan/Rosmarinus officinalis composite nanofibrous membranes with an average diameter of 207 ± 18 nm were successfully prepared using the electrospinning technique. The chemical properties of membranes were analyzed by Fourier transform infrared spectroscopy (FTIR) and the morphological characterization of nanofibers was evaluated with field emission scanning electron microscopy (FE-SEM). Moreover, enzymatic activity of GOx was determined by the Carmine method. FTIR results showed the successful incorporation of glucose oxidase and Rosmarinus officinalis into the nanofiber composite. Immobilized GOx showed high (79.5%) enzymatic activity in the optimum sample. The Rosmarinus officinalis, glucose oxidase-incorporated Nylon–Ag masterbatch/chitosan nanofibrous exhibited excellent antimicrobial activity on both gram-negative bacterium Escherichia coli (97.5%) and gram-positive bacterium Staphylococcus aureus (99.5%). The antibacterial and antioxidant Nylon–Ag masterbatch/chitosan/Rosmarinus officinalis/GOx nanofibrous membrane showed higher potential, compared to the control sample, to be used as food packaging by improving the shelf life and maintaining the quality of food stuffs. Therefore, this research recommends it as a promising candidate for food preservation applications.

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Sivanesan, Iyyakkannu, Judy Gopal, Manikandan Muthu, Juhyun Shin, and Jae-Wook Oh. "Reviewing Chitin/Chitosan Nanofibers and Associated Nanocomposites and Their Attained Medical Milestones." Polymers 13, no. 14 (July 16, 2021): 2330. http://dx.doi.org/10.3390/polym13142330.

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Chitin/chitosan research is an expanding field with wide scope within polymer research. This topic is highly inviting as chitin/chitosan’s are natural biopolymers that can be recovered from food waste and hold high potentials for medical applications. This review gives a brief overview of the chitin/chitosan based nanomaterials, their preparation methods and their biomedical applications. Chitin nanofibers and Chitosan nanofibers have been reviewed, their fabrication methods presented and their biomedical applications summarized. The chitin/chitosan based nanocomposites have also been discussed. Chitin and chitosan nanofibers and their binary and ternary composites are represented by scattered superficial reports. Delving deep into synergistic approaches, bringing up novel chitin/chitosan nanocomposites, could help diligently deliver medical expectations. This review highlights such lacunae and further lapses in chitin related inputs towards medical applications. The grey areas and future outlook for aligning chitin/chitosan nanofiber research are outlined as research directions for the future.

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Modiri-Delshad, Tayebeh, Ali Ramazani, Mehdi Khoobi, Hamid Akbari Javar, Tayebeh Akbari, and Mohsen Amin. "Fabrication of chitosan/polycaprolactone/Myrtus communis L. extract nanofibrous mats with enhanced antibacterial activities." Polymers and Polymer Composites 31 (January 26, 2023): 096739112311515. http://dx.doi.org/10.1177/09673911231151506.

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Development of novel antimicrobial phytochemical-bearing nanofibrous mats could be considered as a promising strategy to overcome against antibiotic resistance in wound healing. In this work, the electrospinning process was used to successfully create novel antimicrobial nanofiber mats made of a blend of electrospun chitosan/polycaprolactone (CS/PCL) loaded with M. communis leaf extract (MCLE) (15 and 30 wt.%). Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) analysis, water contact angle (WCA) testing, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, and mechanical properties testing were applied to evaluate physicochemical properties of the nanofiber mats. The FESEM images showed uniform, bead-free, and smooth nanofiber mats with good compatibility between MCLE and polymers. Image J software was used to calculate the average diameters of nanofibrous mats, and the average diameter increased significantly as the extract concentration increased. The existence of MCLE in the nanofibrous mats was verified by ATR-FTIR spectroscopy and XRD analysis. The tensile strength of the nanofiber mats was satisfactory (6.31–12.47 MPa). The incorporation of MCLE in CS/PCL nanofibers enhanced the scaffold’s hydrophilicity, as evidenced by a reduction in contact angle. Significant reduction up to 0.5 log of both Escherichia ( E.) coli and Staphylococcus aureus count was observed upon exposure to CS/PCL nanofibers. The MCLE (15 and 30 wt.%)-incorporated CS/PCL nanofibers demonstrated a significant reduction of bacterial count up to 0.8 log for both bacteria. The results demonstrated that manufactured nanofibers could be considered as a promising dressing in wound dressing.

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Milanesi, Giulia, Barbara Vigani, Silvia Rossi, Giuseppina Sandri, and Elisa Mele. "Chitosan-Coated Poly(lactic acid) Nanofibres Loaded with Essential Oils for Wound Healing." Polymers 13, no. 16 (August 4, 2021): 2582. http://dx.doi.org/10.3390/polym13162582.

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Chronic skin wounds are characterised by a non-healing process that makes necessary the application of wound dressings on the damaged area to promote and facilitate the recovery of skin’s physiological integrity. The aim of the present work is to develop a bioactive dressing that, once applied on the injured tissue, would exert antibacterial activity and promote adhesion and proliferation of fibroblasts. Nanofibres consisting of poly(lactic acid) (PLA) and essential oils (EOs) were electrospun and coated with a medium molecular weight chitosan (CS). Black pepper essential oil (BP-EO) or limonene (L), well-known for their antibacterial properties, were added to the PLA/acetone solution before electrospinning; phase separation phenomena occurred due to the poor solubility of the EOs in the PLA solution and led to fibres having surface nano-pores. The porous electrospun fibres were coated with CS to produce hydrophilic membranes that were easy to handle, biocompatible, and suited to promote cellular proliferation. The fibrous scaffolds were tested in terms of mechanical resistance, wettability, antibacterial activity, in-vitro cytotoxicity, and ability to promote fibroblasts’ adhesion and proliferation. The results obtained proved that the CS coating improved the hydrophilicity of the fibrous mats, enhanced EO’s antibacterial potential, and promoted cell adhesion and proliferation.

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Park, Jun-Yong, Kyu-Hong Kyung, Kosuke Tsukada, Sae-Hoon Kim та Seimei Shiratori. "Biodegradable polycaprolactone nanofibres with β-chitosan and calcium carbonate produce a hemostatic effect". Polymer 123 (серпень 2017): 194–202. http://dx.doi.org/10.1016/j.polymer.2017.07.013.

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Nthumbi, Richard M., J. Catherine Ngila, Brenda Moodley, Andrew Kindness, and Leslie Petrik. "Application of chitosan/polyacrylamide nanofibres for removal of chromate and phosphate in water." Physics and Chemistry of the Earth, Parts A/B/C 50-52 (2012): 243–51. http://dx.doi.org/10.1016/j.pce.2012.07.001.

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Çay, Ahmet, Mohsen Miraftab, and E. Perrin Akçakoca Kumbasar. "Characterization and swelling performance of physically stabilized electrospun poly(vinyl alcohol)/chitosan nanofibres." European Polymer Journal 61 (December 2014): 253–62. http://dx.doi.org/10.1016/j.eurpolymj.2014.10.017.

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Guarino, Vincenzo, Iriczalli Cruz-Maya, Rosaria Altobelli, W. K. Abdul Khodir, Luigi Ambrosio, Marco A. Alvarez Pèrez, and Argelia Almaguer Flores. "Electrospun polycaprolactone nanofibres decorated by drug loaded chitosan nano-reservoirs for antibacterial treatments." Nanotechnology 28, no. 50 (November 23, 2017): 505103. http://dx.doi.org/10.1088/1361-6528/aa9542.

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Li, Ai D., Z. Z. Sun, M. Zhou, X. X. Xu, J. Y. Ma, W. Zheng, H. M. Zhou, L. Li, and Y. F. Zheng. "Electrospun Chitosan-graft-PLGA nanofibres with significantly enhanced hydrophilicity and improved mechanical property." Colloids and Surfaces B: Biointerfaces 102 (February 2013): 674–81. http://dx.doi.org/10.1016/j.colsurfb.2012.09.035.

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Van der Schueren, Lien, Iline Steyaert, Bert De Schoenmaker, and Karen De Clerck. "Polycaprolactone/chitosan blend nanofibres electrospun from an acetic acid/formic acid solvent system." Carbohydrate Polymers 88, no. 4 (May 2012): 1221–26. http://dx.doi.org/10.1016/j.carbpol.2012.01.085.

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Van der Schueren, Lien, Thierry De Meyer, Iline Steyaert, Özgür Ceylan, Karen Hemelsoet, Veronique Van Speybroeck, and Karen De Clerck. "Polycaprolactone and polycaprolactone/chitosan nanofibres functionalised with the pH-sensitive dye Nitrazine Yellow." Carbohydrate Polymers 91, no. 1 (January 2013): 284–93. http://dx.doi.org/10.1016/j.carbpol.2012.08.003.

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Cai, Dai-Lun, Dinh Thi Hong Thanh, Pau-Loke Show, Su-Chun How, Chen-Yaw Chiu, Michael Hsu, Shir Reen Chia, Kuei-Hsiang Chen, and Yu-Kaung Chang. "Studies of Protein Wastes Adsorption by Chitosan-Modified Nanofibers Decorated with Dye Wastes in Batch and Continuous Flow Processes: Potential Environmental Applications." Membranes 12, no. 8 (August 1, 2022): 759. http://dx.doi.org/10.3390/membranes12080759.

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In this study, reactive green 19 dye from wastewater was immobilized on the functionalized chitosan nanofiber membranes to treat soluble microbial proteins in biological wastewater. Polyacrylonitrile nanofiber membrane (PAN) was prepared by the electrospinning technique. After heat treatment, alkaline hydrolysis, and chemically grafted with chitosan to obtain modified chitosan nanofibers (P-COOH-CS), and finally immobilized with RG19 dye, dyed nanofibers were generated (P-COOH-CS-RG19). The synthesis of P-COOH-CS and P-COOH-CS-RG19 are novel materials for protein adsorption that are not deeply investigated currently, with each of the material functions based on their properties in significantly improving the adsorption efficiency. The nanofiber membrane shows good adsorption capacity and great recycling performance, while the application of chitosan and dye acts as the crosslinker in the nanofiber membrane and consists of various functional groups to enhance the adsorption of protein. The dyed nanofibers were applied for the batch adsorption of soluble protein (i.e., lysozyme), and the process parameters including chitosan’s molecular weight, coupling pH, chitosan concentration, dye pH, dye concentration, and lysozyme pH were studied. The results showed that the molecular weight of chitosan was 50 kDa, pH 5, concentration 0.5%, initial concentration of dye at 1 mg/mL dye and pH 12, lysozyme solution at 2 mg/mL at pH 8, and the maximum adsorption capacity was 1293.66 mg/g at a temperature of 318 K. Furthermore, thermodynamic, and kinetic studies suggested that the adsorption behavior of lysozyme followed the Langmuir adsorption isotherm model and the pseudo-second-order kinetic model. The optimal adsorption and desorption conditions based on batch experiments were directly applied to remove lysozyme in a continuous operation. This study demonstrated the potential of dyed nanofibers as an efficient adsorbent to remove approximately 100% of lysozyme from the simulated biological wastewater.

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Safari, Javad, Sara Esteghlal, Malihe Keramat, and Mohammadreza Khalesi. "Fabrication of Chitosan/Pectin/PVA Nanofibers Using Electrospinning Technique." Nanoscience & Nanotechnology-Asia 10, no. 2 (February 25, 2020): 134–41. http://dx.doi.org/10.2174/2210681208666181002124634.

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Background: Electrospinning is a novel cost effective technique for generating nanofibers from a broad range of materials likely to be used as a coating film. Methods: In this project, pectin and chitosan solutions containing PVA were prepared and electrospun with separate syringes for the first time. The antimicrobial and physical properties of the novel chitosan/PVApectin/ PVA nanofibrous film were evaluated using some analysis techniques such as disc diffusion assay, scanning electron microscopy (SEM), transmission electron microscopy (TEM), viscosity and conductivity tests, and fourier-transform infrared spectroscopy (FTIR). Results: The results showed that simultaneously electrospinning the dispersion of chitosan/PVA (50:50) with pectin/PVA (50:50) led to the formation of thin nanofibers with the minimum number of beads. The results of FTIR analysis proved the dispersion of chitosan and PVA in nanofiber mats and the interaction of chitosan with pectin as well as PVA with pectin. Disc diffusion assay showed that nano-film could possess significant antibacterial activity against S. aureus at 37°C but had no effects against E. coli. Conclusion: Based on the results of this study, the novel chitosan/PVA-pectin/PVA nanofibrous film can be considered as a novel coating film for promising application in food packaging industry.

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Gonçalves, Melissa Marques, Kelsey Leonard Lobsinger, Jaqueline Carneiro, Guilherme Fadel Picheth, Cassiano Pires, Cyro Ketzer Saul, Daniela Florencio Maluf, and Roberto Pontarolo. "Morphological study of electrospun chitosan/poly(vinyl alcohol)/glycerol nanofibres for skin care applications." International Journal of Biological Macromolecules 194 (January 2022): 172–78. http://dx.doi.org/10.1016/j.ijbiomac.2021.11.195.

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Roozbahani, Fatemeh, Naznin Sultana, Davood Almasi та Farnaz Naghizadeh. "Effects of Chitosan Concentration on the Protein Release Behaviour of Electrospun Poly(ε-caprolactone)/Chitosan Nanofibers". Journal of Nanomaterials 2015 (2015): 1–11. http://dx.doi.org/10.1155/2015/747420.

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Poly(ε-caprolactone)/chitosan (PCL/chitosan) blend nanofibers with different ratios of chitosan were electrospun from a formic acid/acetic acid (FA/AA) solvent system. Bovine serum albumin (BSA) was used as a model protein to incorporate biochemical cues into the nanofibrous scaffolds. The morphological characteristics of PCL/chitosan and PCL/chitosan/BSA Nanofibers were investigated by scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) was used to detect the presence of polymeric ingredients and BSA in the Nanofibers. The effects of the polymer blend ratio and BSA concentration on the morphological characteristics and consequently on the BSA release pattern were evaluated. The average fiber diameter and pore size were greater in Nanofibers containing BSA. The chitosan ratio played a significant role in the BSA release profile from the PCL/chitosan/BSA blend. Nanofibrous scaffolds with higher chitosan ratios exhibited less intense bursts in the BSA release profile.

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Schulte-Werning, Laura Victoria, Anjanah Murugaiah, Bhupender Singh, Mona Johannessen, Rolf Einar Engstad, Nataša Škalko-Basnet, and Ann Mari Holsæter. "Multifunctional Nanofibrous Dressing with Antimicrobial and Anti-Inflammatory Properties Prepared by Needle-Free Electrospinning." Pharmaceutics 13, no. 9 (September 21, 2021): 1527. http://dx.doi.org/10.3390/pharmaceutics13091527.

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An active wound dressing should address the main goals in wound treatment, which are improved wound healing and reduced infection rates. We developed novel multifunctional nanofibrous wound dressings with three active ingredients: chloramphenicol (CAM), beta-glucan (βG) and chitosan (CHI), of which βG and CHI are active nanofiber-forming biopolymers isolated from the cell walls of Saccharomyces cerevisiae and from shrimp shells, respectively. To evaluate the effect of each active ingredient on the nanofibers’ morphological features and bioactivity, nanofibers with both βG and CHI, only βG, only CHI and only copolymers, polyethylene oxide (PEO) and hydroxypropylmethylcellulose (HPMC) were fabricated. All four nanofiber formulations were also prepared with 1% CAM. The needle-free NanospiderTM technique allowed for the successful production of defect-free nanofibers containing all three active ingredients. The CAM-containing nanofibers had a burst CAM-release and a high absorption capacity. Nanofibers with all active ingredients (βG, CHI and CAM) showed a concentration-dependent anti-inflammatory activity, while maintaining the antimicrobial activity of CAM. The promising anti-inflammatory properties, together with the high absorption capacity and antimicrobial effect, make these multifunctional nanofibers promising as dressings in local treatment of infected and exuding wounds, such as burn wounds.

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Islam, Md Shariful, Fayeka Mansura, Amalina Muhammad Afifi, and Bee Chin Ang. "Fabrication and Characterization of Poly(Vinyl Alcohol)/Chitosan Blend Electrospun Nanofibrous Membrane." Key Engineering Materials 701 (July 2016): 265–69. http://dx.doi.org/10.4028/www.scientific.net/kem.701.265.

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In this study, poly (vinyl alcohol) / chitosan blend nanofibers were synthesized by electrospinning process in different polyvinyl alcohol and chitosan weight ratios. The nanofibers were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) and X-ray diffraction (XRD). SEM images showed that, 50:50 poly vinyl alcohol/chitosan blend was the ideal ratio for producing beadless nanofiber. The average diameter of the beadless nanofiber was found to be 123 nm. FTIR and XRD results demonstrated the presence of intermolecular hydrogen bonding between the molecules of poly vinyl alcohol and chitosan.

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Wu, Jheng-Yu, Chi-Yun Wang, Kuei-Hsiang Chen, You-Ren Lai, Chen-Yaw Chiu, Hung-Che Lee, and Yu-Kaung Chang. "Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy." Membranes 12, no. 3 (March 17, 2022): 332. http://dx.doi.org/10.3390/membranes12030332.

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N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC) is a type of quaternary ammonium chitosan derivative with an antibacterial activity superior to the pristine chitosan, but its electrospinnability is limited. In this study, polyvinyl alcohol (PVA) was blended with HTCC to improve the electrospinnability of nanofibers. The electrospinning of PVA–HTCC nanofiber membranes was optimized in terms of structural stability and antimicrobial performance. Based on scanning electron microscopic analysis, the morphology and diameter of the produced nanofibers were influenced by the applied voltage, flow rate of the feed solution, and weight ratio of the polymer blend. An increase in the HTCC content decreased the average nanofiber diameter. The maximum water solubility of the PVA–HTCC nanofibers reached the maximum value of 70.92% at 12 h and 25 °C. The antibacterial activity of PVA–HTCC nanofiber membranes against Escherichia coli was ~90%, which is significantly higher than that of PVA–chitosan nanofiber membrane. Moreover, the antibacterial efficiency of PVA–HTCC nanofiber membranes remained unaffected after 5 cycles of antibacterial treatment. The good antibacterial performance and biocompatibility of PVA–HTCC nanofiber membrane makes them attractive for biomedical and biochemical applications that necessitate sterile conditions.

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Zhang, Ping, and Shan Shan Wu. "Nanofibers/PVA Blended Nano Fibre Matrix for Nervous Tissue Regeneration." Applied Mechanics and Materials 404 (September 2013): 95–99. http://dx.doi.org/10.4028/www.scientific.net/amm.404.95.

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Nanofibers produced by electrospinning represent a new class of promising scaffolds to support nerve regeneration. Here, we found that the blended solutions of chitosan (CS) with Poly (vinyl alcohol) (PVA) are appropriate for electrospinning when they form conductive, unstructured fluids displaying plasticity, rather than elasticity, in the bulk and at the interface. We then studied that utilize electrospun nanofibers to manipulate biological processes relevant to nervous tissue regeneration, including stem cell differentiation, guidance of neurite extension, and peripheral nerve injury treatments. The main objective of this article is to provide valuable methods for investigating the mechanisms of neurite growth on novel nanofibrous scaffolds and optimization of the nanofiber scaffolds and conduits for repairing peripheral nerve injuries.

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Mokhena, Teboho Clement, and Adriaan Stephanus Luyt. "Development of multifunctional nano/ultrafiltration membrane based on a chitosan thin film on alginate electrospun nanofibres." Journal of Cleaner Production 156 (July 2017): 470–79. http://dx.doi.org/10.1016/j.jclepro.2017.04.073.

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Shabunin, Anton, Vladimir Yudin, Irina Dobrovolskaya, Evgeny Zinovyev, Viktor Zubov, Elena Ivan’kova, and Pierfrancesco Morganti. "Composite Wound Dressing Based on Chitin/Chitosan Nanofibers: Processing and Biomedical Applications." Cosmetics 6, no. 1 (March 1, 2019): 16. http://dx.doi.org/10.3390/cosmetics6010016.

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An electrospinning technique was used for the preparation of a bilayered wound dressing consisting of a layer of aliphatic copolyamide nanofibers and a layer of composite nanofibers from chitosan and chitin nanofibrils filler. Processed dressings were compared with aliphatic copolyamide nanofiber-based wound dressings and control groups. Experimental studies (in vivo treatment of third-degree burns with this dressing) demonstrated that almost complete (up to 97.8%) epithelialization of the wound surface had been achieved within 28 days. Planimetric assessment demonstrated a significant acceleration of the wound healing process. Histological analysis of scar tissue indicated the presence of a significant number of microvessels and a low number of infiltrate cells. In the target group, there were no deaths or purulent complications, whereas in the control group these occurred in 25% and 59.7% of cases, respectively—and, in the copolyamide group, 0% and 11%, respectively. The obtained data show the high efficiency of application of the developed composite chitosan‒copolyamide wound dressings for the treatment of burn wounds.

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Sun, Kang, Yuan Xin Ge, Zhong Hui Li, and Xiao Lin Zhang. "Post-Processing of Chitosan Based Nanofibers Prepared by Electrospinning." Advanced Materials Research 873 (December 2013): 652–62. http://dx.doi.org/10.4028/www.scientific.net/amr.873.652.

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Recently, electrospinning of nanofibers based on chitosan has been widely studied and numerous chitosan based nanofibers have been prepared, for the enormous possibilities of applications in various areas such as filtration, enzyme immobilization, tissue engineering, wound dressing, drug delivery, and catalysis. Because most of the chitosan based nanofibers are soluble in aqueous solutions and the other properties such as mechanical properties are not suitable for the further applications. It is necessary to improve the properties of chitosan based nanofibers. Various post-processing has been done to the chitosan based nanofibers and many post-processing products have emerged. This article discusses the post-processing of the chitosan based nanofibers involving methods, mechanisms, changes of nanofiber properties, and applications of the post-processing products in details. The post-processing is divided into alkali treatment, crosslinking, and functionalizing.

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Guerra, Mónica, Fábio F. F. Garrudo, Célia Faustino, Maria Emilia Rosa, and Maria H. L. Ribeiro. "Exploring Functionalized Magnetic Hydrogel Polyvinyl Alcohol and Chitosan Electrospun Nanofibers." Gels 9, no. 12 (December 11, 2023): 968. http://dx.doi.org/10.3390/gels9120968.

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Nanofibrous materials present interesting characteristics, such as higher area/mass ratio and reactivity. These properties have been exploited in different applications, such as drug-controlled release and site-specific targeting of biomolecules for several disease treatments, including cancer. The main goal of this study was to develop magnetized nanofiber systems of lysozyme (Lys) for biological applications. The system envisaged electrospun polyvinyl alcohol (PVA) and PVA/chitosan (CS) nanofibers, loaded with Lys, crosslinked with boronic acids [phenylboronic acid (PBA), including 2-acetylphenylboronic acid (aPBA), 2-formylphenylboronic (fPBA), or bortezomib (BTZ)] and functionalized with magnetic nanobeads (IONPs), which was successfully built and tested using a microscale approach. Evaluation of the morphology of nanofibers, obtained by electrospinning, was carried out using SEM. The biological activities of the Lys-loaded PVA/CS (90:10 and 70:30) nanofibers were evaluated using the Micrococcus lysodeikticus method. To evaluate the success of the encapsulation process, the ratio of adsorbed Lys on the nanofibers, Lys activity, and in vitro Lys release were determined in buffer solution at pH values mimicking the environment of cancer cells. The viability of Caco-2 cancer cells was evaluated after being in contact with electrospun PVA + Lys and PVA/CS + Lys nanofibers, with or without boronic acid functionalation, and all were magnetized with IONPs.

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Jamnongkan, Tongsai, Amnuay Wattanakornsiri, P. Pungboon Pansila, Claudio Migliaresi, and Supranee Kaewpirom. "Effect of Poly(vinyl alcohol)/Chitosan Ratio on Electrospun-Nanofiber Morphologies." Advanced Materials Research 463-464 (February 2012): 734–38. http://dx.doi.org/10.4028/www.scientific.net/amr.463-464.734.

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Series of poly (vinyl alcohol)/chitosan (PVA/CS) electrospun nanofibers with different weight ratio of PVA and CS were fabricated by electrospinning method. The surface morphology, diameter, and structure of electrospun nanofibers were investigated by scanning electron microscopy (SEM). As a result of PVA and CS composition measurements, the electrospun nanofibers morphologies were mainly affected by weight ratio of the polymer solution. When increasing the chitosan content in the blend solution, the electrospun nanofibers could hardly form. This result indicates that the electrospun nanofiber formation is enhanced by chitosan content.

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Istiqomah, Kholli Vatul Nur, and Diah Hari Kusumawati. "SINTESIS NANOFIBER KITOSAN/PVA SEBAGAI WOUND DRESSING DENGAN METODE ELEKTROSPINNING." Inovasi Fisika Indonesia 11, no. 1 (February 10, 2022): 1–7. http://dx.doi.org/10.26740/ifi.v11n1.p1-7.

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Abstrak Nanofiber kitosan/PVA dapat digunakan sebagai wound dressing karena memiliki sifat bioaktif dan biokompatibel. Pembuatan nanofiber dilakukan dengan menggunakan metode elektrospinning. Penelitian ini menggunakan larutan kitosan dengan konsentrasi 3% dan larutan PVA dengan konsentrasi 10%. Pencampuran larutan kitosan dengan larutan PVA mengunakan perbandingan volume:volume yaitu 1:4, 2:4 dan 3:4. Selanjutnya dilakukan proses elektrospinning dengan parameter meliputi tegangan 20 kV, jarak jarum ke kollektor 15 cm, serta laju alir 5 ml/jam. Nanofiber yang dihasilkan dari proses elektrospinning kemudian dikarakterisasi menggunakan Fourier Transform Infrared (FTIR) yang berfungsi untuk melihat gugus fungsi yang terdapat pada sampel, Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX) yang berfungsi untuk melihat morfologi dan material penyusun, dan X-Ray Diffraction (XRD) berfungsi untuk mengidentifikasi fasa kristalin dalam material. Nanofiber yang dihasilkan menunjukkan nanofiber mengandung gugus kitosan dan PVA yang dibuktikan adanya kemiripan spektrum antara nanofiber kitosan/PVA dengan senyawa kitosan dan PVA. Nanofiber kitosan/PVA 1:4 dapat digunakan sebagai wound dressing karena membentuk nanofiber lebih baik dibanding lainnya, dimana fibers yang dihasilkan homogen dengan ukuran fiber yang hampir sama yaitu 177,1 nm, rapat, dan permukaannya halus tanpa adanya beads yang dibuktikan dengan karakterisasi SEM. Kata Kunci: kitosan, elektrospinning, nanofiber, wound dressing Abstract The manufacture of nanofibers was carried out using the electrospinning method. This study used a chitosan solution with a concentration of 3% and a PVA solution with a concentration of 10%. Mixing the chitosan solution with the PVA solution used a volume: volume ratio of 1:4, 2:4 and 3:4. Furthermore, the electrospinning process was carried out with parameters including a voltage of 20 kV, a needle to the collector of 15 cm, and a flow rate of 5 ml/hour. The nanofibers produced from the electrospinning process were then characterized using Fourier Transform Infrared (FTIR) which serves to see the functional groups contained in the sample, Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX) which functions to see the morphology and constituent materials, and X-Ray Diffraction (XRD) serves to identify the crystalline phase in the material The spectrum of similarities between nanofiber chitosan/PVA with chitosan and PVA compounds proves that the produced nanofiber contains the chitosan and PVA group. Chitosan/PVA 1:4 nanofibers can be used as wound dressings because they form nanofibers better than others, where the resulting fibers are homogeneous with almost the same fiber size, namely 177.1 nm, tight, and smooth surface without any beads as evidenced by SEM characterization. Keywords: chitosan, electrospinning, nanofiber , wound dressing

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