Дисертації з теми "N mouse"
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Lommel, Silvia. "Conditional inactivation of N-WASP in the mouse analyses of N-WASP function /." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964801922.
Повний текст джерелаDagälv, Anders. "Role of Heparan Sulfate N-sulfation in Mouse Embryonic Development." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-123474.
Повний текст джерелаMazza, Graziella. "Antibody responses to Schistosoma mansoni in the CBA/N mouse." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303138.
Повний текст джерелаBarron, Catherine Mary. "Effects of Trimethylamine N-Oxide on Mouse Embryonic Stem Cell Properties." Thesis, Virginia Tech, 2020. http://hdl.handle.net/10919/99602.
Повний текст джерелаMaster of Science
Trimethylamine N-oxide (TMAO) is a metabolite that is produced by the bacteria in the gut after the consumption of specific dietary ingredients (e.g., choline, carnitine, betaine). These ingredients are commonly found in meat and dairy products, and thus make up a large part of the average American diet. Recently, it was discovered that high TMAO levels in the bloodstream put people at an increased risk for heart disease, neurodegenerative diseases (e.g., Alzheimer's Disease), diabetes, stroke, and chronic kidney disease. At the cellular level, there is evidence that TMAO increases inflammation and the production of oxygen radicals, which causes cells to lose their function and promotes the onset of disease. TMAO has been well studied in adult cell types; however, no one has investigated whether TMAO will impact cells of the early embryo. This project aims to explore the impact of TMAO on mouse embryonic stem cells (mESCs), which are cells that represent the early stage of embryonic development and are critical for proper development of the final offspring. In addition, mESCs may also help to provide insight into how TMAO impacts other stem cell types, some of which are present throughout the entire human lifespan and play an important role in the body's ability to repair itself and maintain overall health. My project demonstrated that TMAO does not impact the overall health of mESCs under normal conditions, which signifies that TMAO generated by a pregnant mother may not directly impact the early embryonic stage of development. Further studies should be conducted to determine the potential impact of TMAO on late stages of embryonic and fetal development. Next, to simulate diseased conditions, the mESCs were treated with extremely high concentrations of TMAO in order to determine what concentration of TMAO will negatively impact these cells. It was found that at 5mM TMAO, mESCs begin to lose their basic properties and become dysfunctional. They are impaired in their viability, growth, ability to become other cell types, and in their metabolic activity. These mESC properties are shared with several types of adult stem cells, and therefore, these findings help to provide insight into how TMAO may impact stem cells found in the adult body which are exposed to a lifetime of high TMAO levels. In the future, we would like to further explore the impact of TMAO on mESCs at the molecular level as well as examine the direct impact of TMAO on other stem cell types.
Ly, Lan H. "Immunosuppressive dietary n-3 polyunsaturated fatty acids differentially modulate costimulatory regulation of murine CD4+ T-cell function." Texas A&M University, 2004. http://hdl.handle.net/1969.1/1535.
Повний текст джерелаMunro, Sandra Bronwen. "Characterization of a composite cDNA clone encoding mouse testicular N-Cadherin and the mouse homologue of a human breast tumor autoantigen." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69645.
Повний текст джерелаThe cadherins, the influenza strain A hemagglutinins, and the fibroblast growth factor receptors are three different families of integral membrane glycoproteins that harbour the amino acid motif histidine-alanine-valine (HAV) in regions involved in protein-protein interactions. In order to identify other proteins that possess the HAV motif in functionally important regions, the SwissProt database was searched using a consensus sequence derived from the cadherins, influenza strain A hemagglutinins, and fibroblast growth factor receptors. This search identified the $ alpha$ chains of the HLA class I histocompatibility antigens as a fourth family of integral membrane glycoproteins with an HAV-containing region that is involved in a protein-protein interaction. (Abstract shortened by UMI.)
Yu-Plant, Violeta Lynn. "The development of gene targeted mouse strains for studying arylamine N-acetyltransferase function." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ34082.pdf.
Повний текст джерелаNeale, Sondra-Ann. "Characterization of the neural cell adhesion molecule N-CAM in splotch mutant mouse embryos." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69646.
Повний текст джерелаA new genotyping assay was also implemented for examination of N-CAM in Sp and other related wildtype strains.
Rago, Luciano F. [Verfasser], and Rolf [Akademischer Betreuer] Kemler. "N-cadherin expression regulation by microRNAs in the mouse developing neocortex = N-Cadherin-Expressionsregulation von microRNAs in der Maus entwickelt Neokortex." Freiburg : Universität, 2014. http://d-nb.info/1122646674/34.
Повний текст джерелаMuraveika, Liudmila [Verfasser], and Daniela N. [Akademischer Betreuer] Männel. "Binding specificity of mouse ficolin to different bacterial strains / Liudmila Muraveika. Betreuer: Daniela N. Männel." Regensburg : Universitätsbibliothek Regensburg, 2012. http://d-nb.info/1028392583/34.
Повний текст джерелаDomke, Tanja Carolina Elisabeth. "O-linked N-acetylglucosamine in differentiation and gene expression of mouse and human pluripotent stem cells." Thesis, University of Dundee, 2016. https://discovery.dundee.ac.uk/en/studentTheses/e84ff9a1-a4ab-41d0-828c-60a191b42c69.
Повний текст джерелаDavis, Jada Leanne-Bittle. "The role of redox dysregulation in the effects of prenatal stress on the embryonic and adult mouse brain." Diss., University of Iowa, 2018. https://ir.uiowa.edu/etd/6562.
Повний текст джерелаLucey, Michelle M. "The expression of the High Mobility Group N (HMGN) family of proteins during development of the mouse eye." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 120 p, 2008. http://proquest.umi.com/pqdweb?did=1597629771&sid=19&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Повний текст джерелаBerjanskii, Mark. "Structure and dynamics of the N-terminal J-domain of T antigens of murine polyomavirus." MU online access free, to others for fee Free online access, 2002. http://wwwlib.umi.com/cr/mo/preview?3052146.
Повний текст джерелаRingvall, Maria. "Functions of Heparan Sulfate During Mouse Development : Studies of Mice with Genetically Altered Heparan Sulfate Biosynthesis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4244.
Повний текст джерелаFernandes, Hugo. "A melatonina e seu efeito citoprotetor na maturação de oócitos murinos." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/74/74131/tde-03022016-153822/.
Повний текст джерелаMany factors are involved in the control of oocyte maturation and developmental competence. Melatonin (MEL) is a hormone showing varied functions including antioxidant and antiapoptotic activities, besides influencing many cell signaling pathways. There are few studies on the role of MEL in oocyte maturation and the mouse, due to its quick reproduction and lower maintencance cost, is an interesting model widely used for in vitro and particularly in vivo studies. The aim of this work was to study the effects of MEL on in vivo and in vitro maturation and its cytoprotective action (antioxidant/antiapoptotic) in murine cumulus-oocyte complexes (CCOs). In experiment one, mice received MEL injections at concentrations of 0 (control), 10 and 20 mg/kg/i.p./day for 4 days. CCOs were in vivo matured and recovered 17 hours after the last injection. In experiment 2, the animals received MEL in the same dosages of the previous experiment, but for 3 days. CCOs were collected 24 hours after the last injection and in vitro matured with follicle stimulating hormone (FSH). In experiment 3, CCOs were in vitro matured with three MEL concentrations (10-9, 10-6 e 10-3 M) or FSH (FSH control). Finally, in the fourth experiment, the best concentration of MEL (10-9 M) selected in experiment 3 was used alone or in association with hydrogen peroxide (300 µM; H2O2). CCOs were matured as in the previous experiment. For all four experiments maturation rates were evaluated by extrusion of the first polar body and the expression of genes related to apoptosis (Bax and Bcl2l1) and antioxidant enzymes (Gpx1, Sod1 and Sod2) by qPCR-RT both cumulus cells (CC), and for the oocytes (OO) were assessed as well. In experiment 1, treatment with 20 mg/kg MEL showed a higher rate of in vivo maturation of 80.3%, followed by the control (69.4%) and 10 mg/kg MEL (62.4%; P>0.05). No effect for gene expression treatments (P>0.05) was observed. In experiment 2, maturation rate ranged from 39 to 53.2% between treatments (P>0.05). In CC, the gene expression was reduced for Bcl2l1 and enhanced for Gpx1 in animals treated with 20 mg/kg MEL (P<0.05). For OO, only Gpx1 expression was increased for both MEL treatments (P<0.05). In experiment 3, the maturation rates were 48.9, 53.7, 56 e 57.3% for MEL 10-3, 10-6, 10-9 M and FSH, respectively (P>0.05). MEL concentrations of 10-6 and 10-9 M increased expression of Gpx1 and Sod1 genes in CC (P<0.05). For OO, only Bax increased the gene expression in 10-6 M MEL concentration (P<0.05). In the last experiment, there was no significant difference in maturation rate, ranging from 51.8 for H2O2 to 60% for FSH control (P>0.05). Gpx1 and Sod1 genes had their expression increased in all the treatments in CC (P<0.05). For Bcl2l1 gene, the expression was decreased in CC as well (P<0.05). Based on these data, we conclude that MEL treatment in vivo was unable to promote maturation rate in vivo and in vitro, but under in vitro conditions it induced meiosis progression in murine oocytes. In addition, Gpx1 and Sod1 antioxidant genes were more expressed in CC than OO in response to MEL treatments in vitro indicating induction of a possible protective effect against in vitro culture conditions.
Tomazini, Ana Paula Inoe. "Ação do N-acetilmuramil-L-alanil-D-isoglutamina (MDP) na regeneração nervosa periférica. Estudo experimental em camundongos." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/10/10137/tde-21092007-170257/.
Повний текст джерелаN-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) was evaluated for its effect on the regenerating peripheral neurons in adult animals. The left sciatic nerve of nine male adult mice C57BL/6J was cut, the proximal and distal nerve stumps were inserted into a polyethylene tube (PT) with 0,76 mm inner diameter, leaving a four mm distance gap. Animals were randomly distributed into two groups and received 2µl of purified preparation of collagen (Vitrogen® 2,4 mg/ml) (COL) or collagen and MDP made up in 1:1 volume ratio, obtaining a 1 mM (COL/MDP) concentration. Other four non-operated animals served as controls (NOR). After four weeks, PT with the regenerated nerve cables (RC), were processed for total myelinated axon counts and myelinated fiber diameter. The results revealed significant difference (p<0.05) in the axons count among the groups NOR (4355 ± 32), COL (1869 ± 289) and COL/MDP (2430 ± 223). There was a significant reduction in the diameter of myelinated fibers in the operated groups (COL = 3,38 µm ± 1,16 and COL/MDP = 3,54 µm ± 1,16) when compared to non-operated animals (6,19 µm ± 2,45). The L5 dorsal root ganglion (DRG) was also removed form the same mice and serially sectioned for sensory neurons count and measurement. No difference was found in the number of DRG neurons among the experimental groups (COL = 564 ± 51 and COL/MDP = 514 ± 56), which presented fewer sensory neurons compared to non-operated group (NOR = 1097 ± 142). The results indicate that the locally applied MDP stimulates peripheral nerve regeneration in mice.
Ferreira, Larissa Domenegueti. "Efeito do S-nitroso-N-acetilpenicilamina sobre receptores vaniloide de potencial transitório 1 em córnea de camundongos." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17150/tde-05012017-093309/.
Повний текст джерелаTRPV-1 receptors are non-selective nocioceptive cation channels. They play a role in cornea sensation. In the present work we describe the response of corneas of TRPV1-/- and mice to chemical stimuli and the effect of SNAP on this receptor. TRPV1-/- and C57BL/6 mice were compared. The observations included the calcium influx in culture, direct observation and the behavior of eye wipe after corneal challenge with 1?M Capsaicin (CAP) with or not 1mM SNAP eye drops. Ocular surface characteristics were not different between both genotypes, except that the sensitivity to CAP is lower in TRPV1-/- (p=0.0019 and 0.0095, respectively). Immunostaining revealed that TRPV 1 is expressed in the basal layer of the cornea epithelia, only in the C57 mice. CAP stimulated calcium influx in epithelial cells in culture and cornea sensitivity in C57 mice in vivo was reduced in the presence of SNAP (p=0.0329 and 0.01, respectively). The absence of TRPV-1 receptors in the cornea epithelia did not affect the ocular surface phenotype in mice. The response to SNAP eye drops revealed that it could work as an analgesic therapy due to its antagonism to the TRPV1.
Bharadwaj, Rahul. "Regulation of Higher Order Chromatin at GRIN2B and GAD1 Genetic Loci in Human and Mouse Brain: A Dissertation." eScholarship@UMMS, 2013. https://escholarship.umassmed.edu/gsbs_diss/651.
Повний текст джерелаBharadwaj, Rahul. "Regulation of Higher Order Chromatin at GRIN2B and GAD1 Genetic Loci in Human and Mouse Brain: A Dissertation." eScholarship@UMMS, 2002. http://escholarship.umassmed.edu/gsbs_diss/651.
Повний текст джерелаSIMON, Caroline Ferreira. "Avaliação da histotoxicidade e de alterações metabólicas após o uso do etil-cianoacrilato e n-butil cianoacrilato em camundongos." Universidade Federal de Pelotas, 2011. http://repositorio.ufpel.edu.br/handle/ri/2571.
Повний текст джерелаThe cyanoacrylate derivatives have been extensively used as tissue adhesives in wound. Among the most widely used adhesives are the n-butyl cyanoacrylate , which is a compound of long chain and the ethyl-cyanoacrylate which is an acid cyanoacrilic ester of short chain. The objective of this study was evaluated the hepatic, renal and cutaneous toxicity of ethyl-cyanoacrylate and n-butyl cyanoacrylate in mouse. 84 mouse of swiss albino strain were used and divided in three groups, each with 28 animals. A longitudinal incision was made to three centimeters on the back of each mouse. In these animals, the syntheses was realized with ethyl-cyanoacrylate (group A), with n-butyl cyanoacrylate (group B) and suture with náilon monofilament yarn (group C), the control group. Sample were collected at 7, 15, 30 and 45 days after treatment for biochemical examination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, creatinine, urea and for histopathological examination was collected liver, kidneys and animals skin. During the experiment was significant difference between groups in mean of ALT at 30 (p=0,039) and 45 days (p=0,0391), AST at 45 days (p=0,0000) and creatinine at 15 (p=0,0169) and 45 days (p=0,0062). In the histopathological examination were not observed changes in toxicity indicatives in the kidneys, liver, skin. In the studied condition not been demonstrated kidney or liver toxicity in the use of ethyl-cyanoacrylate and n-butyl cyanoacrylate for skin syntheses.
Os derivados dos cianoacrilatos têm sido muito utilizados como adesivos teciduais em feridas. Dentre os adesivos mais utilizados encontram-se o n-butil-cianocrilato , o qual é um composto de cadeia longa e o etil-cianoacrilato que é um éster do ácido cianoacrílico de cadeia curta. O objetivo deste estudo foi avaliar a toxicidade hepática, renal e cutânea dos adesivos etil-cianoacrilato e n-butil cianoacrilato, em camundongos. Foram utilizados 84 camundongos da linhagem swiss albino e divididos em três grupos, cada um com 28 animais. Foi efetuada uma incisão longitudinal de três centímetros no dorso de cada camundongo. Nestes animais, foi depositado no tecido subcutâneo o adesivo etil-cianoacrilato (grupo A), n-butil cianoacrilato (grupo B). No grupo C, considerado grupo controle, foi realizada apenas sutura com fio de náilon monofilamentar. Foram coletadas amostras aos 7, 15, 30 e 45 dias após os tratamentos. Para análise bioquímica, foram avaliados os níveis séricos de Alanina aminotranferase (ALT), Aspartato aminotransferase (AST), fosfatase alcalina, creatinina e uréia. Para o exame histopatológico foi coletado fígado, rins e pele dos animais, os quais foram processados e analisados em microscopia. Durante o experimento houve diferença significativa entre os grupos nos valores médios de ALT aos 30 (p=0,039) e 45 dias (p=0,0391), AST aos 45 dias (p=0,0000) e creatinina aos 15 (p=0,0169) e 45 dias (p=0,0062). No exame histopatológico, não foram observadas alterações indicativas de toxicidade a nível renal, hepático e cutâneo. Nas condições estudadas não foi demonstrado toxicidade renal ou hepática no uso do etil-cianoacrilato e n-butil cianoacrilato para síntese cutânea.
Jiang, Yan. "Chromatin Remodeling in Transgenic Mouse Brain: Implications for the Neurobiology of Depression: A Dissertation." eScholarship@UMMS, 2009. https://escholarship.umassmed.edu/gsbs_diss/423.
Повний текст джерелаNguyen, Nguyen M. "Transgenerational inheritance of increased breast cancer risk in mouse offspring of dams exposed to high fat N-6 polyunsaturated fatty acid diet during pregnancy." Thesis, Georgetown University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10256175.
Повний текст джерелаMaternal high fat (HF) intake before and/or during pregnancy increases female offsprings’ mammary cancer risk in several preclinical models. Here I studied if maternal HF intake during pregnancy cause transgenerational increase in mammary cancer risk, and if the increase is reversible by treating adult offspring with inhibitors of histone deacetylases (HDAC) or DNA methyltransferases (DNMT).
Pregnant C57BL/6NTac mice were fed either a diet high in n-6 polyunsaturated fatty acids (HF) or control diet (CON). HF diet was given from gestational day (GD) 10 – 20 to target fetal primordial germ cell formation and differentiation to germ cells. Offspring in subsequent F1-F3 generations were only fed CON diet. Mammary tumor incidence, induced by 7,12-dimethylbenz[a]anthracene (DMBA), was significantly higher in F1 and F3 HF offspring, than in the controls. Tumor latency was shorter and burden higher in F1 HF, with similar trends, though not statistically significant, in F3 HF.
RNA-sequencing of normal mammary glands revealed 1587 and 4423 differentially expressed genes between HF and CON offspring in F1 and F3, respectively, of which 48 genes were similarly altered in both generations. Ingenuity Pathway Analysis identified genes associated with Notch signaling as key alterations in HF mammary glands. Knowledge-fused Differential Dependency Network analysis identified 10 node genes in HF offspring uniquely connected to genes linked to increased cancer risk, therapy resistance, poor prognosis, and impaired anti-cancer immunity.
Next, I studied whether HDAC and DNMT inhibitor treatment in adulthood of the offspring, prior to tumor formation, could reverse the increased mammary cancer risk caused by in utero HF exposure. CON and HF offspring were given valproic acid and hydralazine in drinking water (epi-treatment), starting one week after tumor initiation by DMBA. Epi-treatment significantly decreased tumor burden in HF offspring, potentially through reactivation of silenced tumor suppressors CLCA1 and CDKN2A, but adversely affected CON offspring. These adverse effects were linked to upregulation of PERK, p62 and HIF-1α in CON.
In summary, maternal HF intake during pregnancy induced transgenerational increase in offsprings’ mammary cancer risk, causes persistent changes in the expression of genes linked to increased breast cancer risk, and epi-treatment in adulthood may reduce this risk.
Richard, Bernhard Clemens [Verfasser], Thomas A. [Akademischer Betreuer] Bayer, and Tiago Fleming [Akademischer Betreuer] Outeiro. "In Vitro and In Vivo Studies on Antibodies: N-terminally Truncated Abeta in the 5XFAD Mouse Model / Bernhard Clemens Richard. Gutachter: Thomas A. Bayer ; Tiago Fleming Outeiro. Betreuer: Thomas A. Bayer." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1071991639/34.
Повний текст джерелаZalavadia, Ankit. "QUANTITATIVE ANALYSIS OF 5-CHLORO-2-METHOXY-N-[2-(4-SULFAMOYLPHENYL)ETHYL]BENZAMIDE (GLYBURIDE ANALOGUE, GA) IN MOUSE PLASMA AND WHOLE BLOOD USING A MICRO-EXTRACTION AND LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4279.
Повний текст джерелаKvist, A. P. (Ari-Pekka). "Type XIII collagen:organization and chromosomal localization of the mouse gene, distance between human COL13A1 and prolyl 4-hydroxylase α-subunit genes, and generation of mice expressing an N-terminally altered type XIII collagen". Doctoral thesis, Oulun yliopisto, 1999. http://urn.fi/urn:isbn:9514253949.
Повний текст джерелаDietrich, Katharina [Verfasser], Thomas A. [Akademischer Betreuer] Bayer, and Uwe-Karsten [Akademischer Betreuer] Hanisch. "Impact of N-terminally truncated Aß4-42 on memory and synaptic plasticity - Tg4-42 a new mouse model of Alzheimer's disease / Katharina Dietrich. Gutachter: Thomas A. Bayer ; Uwe-Karsten Hanisch. Betreuer: Thomas A. Bayer." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/106504481X/34.
Повний текст джерелаVillain, Nicolas. "Rôle de la plasticité comportementale dans l'adaptation aux variations nutritionnelles chez un primate malgache." Thesis, Paris, Muséum national d'histoire naturelle, 2017. http://www.theses.fr/2017MNHN0006/document.
Повний текст джерелаIn order to survive in a changing environment, individuals have to express an appropriate response. It is known that animals have the ability to adjust their behaviour to their environment. This behavioural plasticity allows a quick and adapted response to environmental variations, maximizing the individual'ssurvival and gene transmission. This plasticity relies on costly brain processes making these adaptations particularly dependent of food availability and maybe quality.This thesis project aimed at better understanding the constraints of these responses in a species under a strong selection pressure. To investigate this problematic, we studied the behavioural responses of a small Malagasy primate, the grey mouse lemur (Microcebus murinus), to both quantitative and qualitative changes in food resources. The first part of this work investigated the effect of a short-term caloric restriction without malnutrition over two studies. In the first one, we studied the effects of a 60% caloric restriction without malnutrition on innate behavioural plasticity via the study of the biological clock. The results show a decrease in the ability to resynchronize on a light/dark cycle following a time-shift. This difficulty to resynchronize was linked to body mass loss, the individuals loosing the more weight being the one unable to resynchronize after the 6-hours time shift. In the second study, we investigated the effect of a 40% caloric restriction without malnutrition on acquired behavioural plasticity. This study show a decrease in learning abilities of the restricted individuals after 19 days of dietary treatment and no influence on long term memory. This decrease in learning abilities was also linked with body mass loss, with the individuals loosing the more weight being the one with the worst success rate during this task. The second part focused on the effects of a qualitative variation in food supply via a long-term supplementation with n-3 polyunsaturated fatty acids. This part allowed us to show an increase in learning abilities associated with increased neurogenesis in three brain zones for supplemented animals after 18 month of treatment as well as a decrease of their anxiety level.This thesis work show that both quantitative and qualitative nutritional variations are able to influence different forms of behavioural plasticity and their cerebral basis and are of particular importance in the adaptation and survival of individuals
Allouche, Ahmad. "Validation fonctionnelle d'approches nutritionnelles à allégation "Bien veillir", capables de prévenir le vieillissement cérébral et les maladies neurodégénératives." Thesis, Université de Lorraine, 2012. http://www.theses.fr/2012LORR0316/document.
Повний текст джерелаAlzheimer's disease is a neurodegenerative aging-related dementia that is characterized by loss of memory and cognitive disorders. Toxicity of soluble oligomers of [beta]-amyloid peptide (sOA[beta]) is a key element in early stages of the disease. Absence of curative therapies, chronic aspects of the pathogenic mechanisms implicated and influence of common risk factors shared with the cardiovascular diseases, including dietary parameters and lipid metabolism, should widely encourage considering the interest of preventive interventions allowing slowing the progression and delaying the clinical onset of Alzheimer's-related troubles. Therefore, nutritional approaches could appear as a strategy able to reduce the prevalence of this disease. Early Alzheimer's mouse model allowed us to assess the preventive potential of diets supplemented in docosahexaenoic acid (DHA, C22:6 n-3). Our results show that adequate dietary intake of DHA lead to increased levels in different brain structures. Consequently, hippocampal and cortical synaptic functions were preserved, even upon acute exposure to A[beta] oligomers, maintaining or improving the cognitive capacities of A[beta]-exposed mice. These improvements were positively correlated with DHA-enrichment associated in hippocampus and with preserved synaptic integrity. We also designed nutritional strategies in order to evaluate the beneficial effects of DHA on diet-induced dyslipidemia as well as on cognitive impairment and neurodegenerative processes associated with normal or pathologic aging. Our results show that dietary DHA can prevent high-fat diet-induced dyslipidemia and delay cognitive decline related with normal or pathological aging
Kakarla, Raghavi. "LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC ANALYSIS OF CLINICALLY AND PHARMACOLOGICALLY RELEVANT MOLECULES." Cleveland State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=csu1576196451854014.
Повний текст джерелаRoyo, Julie. "Performances cognitives et neurogenèse au cours du vieillissement chez un primate non-humain." Thesis, Paris, Muséum national d'histoire naturelle, 2020. http://www.theses.fr/2020MNHN0001.
Повний текст джерелаNeurogenesis is the ability of the adult brain to build new neurons. This process induces structural and functional changes in the brain that can reduce cognitive decline during aging. This neuroplasticity exists throughout life but it gradually decreases with aging. In this study, we characterized the evolution of cognitive functions and neurogenesis during aging in the grey mouse lemur (Microcebus murinus) that shares morphological, behavioural and physiological changes with aged humans. We observed that some aged animals presented a specific deficit in learning and memory whereas others had cognitive performances equivalent or better than young animals. It might be due to the neurogenesis process that would preserve cognitive functions during aging. Indeed, in the subventricular zone, the balance between neurons and glial cells would be in favour of neurogenesis in the dorsal part while oligodendrogenesis would be favoured in the horn. Stimulation of neurogenesis could help replace neurons lost due to injury or aging. Among the possible strategies to stimulate neurogenesis, food and physical activity seem pertinent. During this thesis project, we studied, in particular, the impact of n-3 polyunsaturated fatty acid supplementation and the combination of caloric restriction and physical activity in adulthood. These interventions induced an improvement of cognitive functions associated with an increase in the number of new neurons. These different approaches constitute a promising strategy without drugs against cognitive decline during aging by participating in brain plasticity
Rosales, Gerpe María Carla. "The Role of APOBEC3 in Controlling Retroviral Spread and Zoonoses." Thesis, Université d'Ottawa / University of Ottawa, 2014. http://hdl.handle.net/10393/31484.
Повний текст джерелаLloyd, Gemma. "Morse theory for invariant functions and its application to the n-body problem." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506273.
Повний текст джерелаRuiz, Camila Mariana. "Sobre a topologia das singularidades de Morin." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/55/55135/tde-12012016-155424/.
Повний текст джерелаIn this work, we revisit results of T. Fukuda and N. Dutertre and T. Fukui on the topology of Morin maps. In particular, we give a new proof for Dutertre-Fukui\'s Theorem [2, Theorem 6.2] when N = Rn, using Morse Theory for manifolds with boundary. Based on the properties of a gradient n-vector field (∇ f1; : : : ∇ fn) of a Morin map f : M → Rn, where dim M ≥ n, in the second part of this work, we introduce the concept of Morin n-vector field for n-vector fields V = (V1; : : : ; Vn) that are not necessarily gradients. We also generalize the result of T. Fukuda [3, Theorem 1], which establishes a module 2 equivalence between Euler\'s characteristic of a manifold M and Euler\'s characteristic of the singular sets of a Morin map defined on M, to the context of Morin n-vector fields.
Rodriguez, Rodriguez Marisol. "Feminismo e innovaci��n en la narrativa gallega de autor��a femenina: Xohana Torres, Mar��a Xos�� Queiz��n, Carmen Blanco y Teresa Moure." Thesis, University of Auckland, 2009. http://hdl.handle.net/2292/5836.
Повний текст джерелаDivín, Jan. "Měření směrových charakteristik antén." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218898.
Повний текст джерелаAthamena, Ahmed. "N-méthylation de la Phosphatidyléthanolamine, une voie métabolique aux fonctions énigmatiques : caractérisation de la voie dans la moule Mytilus galloprovincialis et rôle physiologique au cours de l’osmorégulation chez les crustacés marins." Thesis, Lyon 1, 2011. http://www.theses.fr/2011LYO10115.
Повний текст джерелаThe specific physiological functions of the N-methylation of phosphatidylethanolamine (PE), one of the two biosynthetic pathways of phosphatidylcholine (PC), remain relatively mysterious. It has been demonstrated in euryhaline fish that hyperosmotic stress induced activation of this pathway in the liver. The aim of our work was to verify whether this phenomenon also occurs in other euryhaline animals. In vivo studies on two species of crabs, Eriocheir sinensis and Carcinus maenas, showed that seawater acclimation activates PC synthesis by N-methylation of PE in the hepatopancreas. Radioisotopic labelling also showed that PC is exchanged with the plasma and that this phenomenon is amplified in animals in seawater. This pool of PC is used as a precursor of betaine, an important organic osmoeffector in these animals. We then characterized the process of PE N-methylation in an osmoconforming animal, the mussel Mytilus galloprovincialis. The results, obtained in vivo and in vitro on isolated tissues, show that N-methylation of PE to PC is expressed in the digestive gland and circulating haemocytes in M. galloprovincialis. The PC synthesized in these tissues is exchanged with hemolymph of the animal. From all these observations, we conclude that the synthesis of PC by N-methylation is widely expressed in marine euryhaline animals and that a physiological function of this pathway is to provide organic osmolytes such as betaine
Derrien, Danièle. "Modulateur de la réponse immune : ciblage par des polymères glycosyles reconnus par les lectines membranaires des macrophages murins." Orléans, 1988. http://www.theses.fr/1988ORLE2037.
Повний текст джерелаStrehl, Britta Katharina. "Struktur und Funktion der 20S Proteasomen aus Organen Listeria monocytogenes infizierter Mäuse." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2005. http://dx.doi.org/10.18452/15276.
Повний текст джерелаThe proteasome system of the cell is responsible for the degradation of proteins and plays a central role in the generation of epitopes which are presented to cytotoxic T-lymphocytes (CTLs) on MHC-class-I molecules. The stimulation of cells by interferon-gamma (IFNgamma) leads to the formation of immunoproteasomes that show an improved generation of many MHC-class-I epitopes compared to constitutive proteasomes. In healthy mice, immunoproteasomes are mainly expressed in the lymphatic tissues, whereas the non-lymphatic organs predominantly contain constitutive proteasomes. In this project the effect of Listeria monocytogenes infection on murine 20S proteasomes derived from the liver, spleen, small intestine and colon were investigated. The structure of the isolated proteasomes was analyzed by two-dimensional gel electrophoresis and western blots while the function was studied by in vitro processing of three oligomeric peptide substrates. Identification and quantification of the processing products was performed by HPLC-ESI-ion trap mass spectrometry. The project showed for the first time, that after infection 20S proteasomes isolated from non-lymphatic organs as well as from non-lymphatic cells displayed structural and functional plasticity: immunoproteasomes were induced post infection which could be correlated with the enhanced generation of immuno-relevant fragments. This was independent of the direct presence of Listeria monocytogenes in the organs and solely controlled by the cytokine IFNgamma. In addition, an increased posttranslational modification with the monosaccharide N-acetylglucosamine could be detected in liver-derived proteasomes after infection. Furthermore, a detailed analysis of the mass spectrometry data was established according to the cleavage site usage of constitutive and immunoproteasomes. The result was that immunoproteasomes are involved in improved generation of the immuno-relevant fragments by the faster cleavage and the changed usage of existing cleavage sites after infection.
Jean, Bruno. "Un polymere thermosensible a l'interface eau-air : interaction avec les tensioactifs et stabilisation de films minces." Paris 6, 2000. http://www.theses.fr/2000PA066230.
Повний текст джерелаHsu, Ren-Jun, and 許仁駿. "Establishment and analysis of (CAG)n transgenic mouse model." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/06769567736631251094.
Повний текст джерела中山醫學大學
醫學研究所
91
Among human trinucleotide repeat disorders, the most frequent triplets found to be expanded are CAG and its complementary sequence, CTG. CAG repeats are almost always found in coding regions whereas CTG expansions are located in untranslated regions (UTRs). Previously we showed that expanded CTG and CAG repeat in the 3’-UTR of GFP both had pathogenic effect in transgenic C. elegans. To investigate the possible pathogenic effect of untranslated (CAG)n trinucleotide repeat in mammals, we made transgenic mice expressing a muscle-specific transcript with (CAG)200 inserted in the 3’-UTR of EGFP gene. The long tract of CAG repeat was found to cause reduced protein expression of EGFP as evidenced by direct fluorescence and western blotting. Histological analysis of the muscle sections revealed atypical muscle cell morphology as well as abnormal staining patterns of succinate dehydrogenase and NADH activity in (CAG)200 mice. Furthermore, mice expressing expanded CAG repeat exhibited a slight deviation in muscle tension recording, suggesting a sign of low muscle activity. By RNA fluorescent in situ hybridization, the muscle cells of (CAG)200 mice were shown to contain nuclear retentions of transcripts, or nuclear foci. Further analysis demonstrated that the expression of muscle differentiation markers, MyoD, Myf5, myogenine and CHCR, were upregulated in the muscle cells of adult (CAG)200 mice. These effects were specific to CAG repeat because mice expressing EGFP RNA without CAG repeat did not differ from the nontransgenic mice in any aspects as mentioned above. This result is the first to show that the expanded CAG repeat in UTR could cause pathogenic effects in mice and suggested that disorders linked to untranslated CAG repeat expansion may exist in human.
Lommel, Silvia [Verfasser]. "Conditional inactivation of N-WASP in the mouse : analyses of N-WASP function / von Silvia Lommel." 2002. http://d-nb.info/964801922/34.
Повний текст джерелаLi, Pei-Tzu, and 李倍慈. "The physiological role of mouse b1,4-N-acetylgalactosaminyl transferase in uterus." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/76441207496126264289.
Повний текст джерела國立清華大學
生物資訊與結構生物研究所
100
Glycosylation is a fundamentally important modification in reproductive physiology, including embryo implantation. The B4galnt2 encodes an enzyme, the Sda β-1,4-N-acetylgalactosaminyltransferase II (β4GalNAcT-II, B4galnt2), which catalyzes the GalNAc linking to the Gal of the NeuAcα2-3Galβ terminal structure via the β-1,4 linkage and forms the sda antigen. In the reproductive system, sda antigen has been found in association with glycoproteins, which are specifically related with pregnancy, such as bovine pregnancy-associated glycoproteins (PAGs), zona pellucida glycoprotein 3 (Zp-3) and glycodelin (Gd). Via hormone treatment in vitro and promoter reporter assay, this study realized that B4galnt2 was positively regulated by progesterone (P4) and negatively regulated by estrogen (E2) in the uteri. These results coincided with in vivo observation. During pregnancy, B4galnt2 is significantly expressed on the intracellular portion of uterine tissue for high levels of P4 at E3.5 and E10.5. This study suggested that the uteri could provide Sda modified protein(s) or lipid(s) on the surface of uterine epithelia at these moments. Using siRNA assay to reduce the B4galnt2 expression in vivo, the reduction of embryonic numbers in uteri revealed the importance of this gene in embryo implantation and development. Furthermore, Bgalnt2 was found to locate on the surface and intracellular portion of E3.5 blastocysts, via confocal microscopy observation. The suppression of blastocyst adhesion by the anti-B4galnt2 antibody and lectin, Dolichos biflous agglutinin (DBA), in vitro and in vivo, was demonstrated by the involvement of B4galnt2 in embryonic early implantation. These results suggested that B4galnt2 on the blastocyst membrane surface may be associated with the sda antigen containing protein(s) or lipid(s) on the uterine epithelium. In addition, the sda antigen on the blastocyst membrane surface bound the receptor/acceptor on the endometrial surface to permit early embryo implantation jointly.
Lin, Shih-Wei, та 林仕韋. "Functional characterization of β-1,4-N-acetyl-galactosaminyl transferase Ⅱ protein in mouse ovary". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/24249444616208476461.
Повний текст джерела國立臺灣大學
生化科學研究所
101
Sda β-1,4-N-acetyl-galactosaminyl transferase Ⅱ(β4galNAcTⅡ,B4GALNT2 in human and B4galnt2 in mouse) catalyze the addition of GalNAc to the Gal of the [Neu5Acα2-3]Galβ1-4GlcNAcβ1 -3Gal terminal structure via the β-1,4 linkage to form Sda antigen. The Sda antigen was first reported as a human blood surface antigen, and in the later researches, it was found in several tissue types, including stomach, colon, kidney, and oocytes. Its presence was also demonstrated in various body fluids, such as saliva, milk, serum and urine. Current studies revealed that Sda antigen reduced remarkably in cancer lesions of the gastrointestinal tract. It indicated the significance of this antigen in biosystem and also associated it is importance to B4galnt2. In 2003, Dell’s group found Sda antigen in reproductive system firstly, however, the significance of Sda is still unclear. My research intends to approach biological characteristics of B4galnt2, and on it, can investigate physiological role of Sda in reproductive system. Female ICR mouse is selected as an animal model. B4galnt2 expression was detected in ovary, and Sda antigen was further confirmed in oocyte but not in cumulus cell. Based on that, Immunohistochemical analysis was used to investigate B4galnt2 distribution in whole cumulus-oocyte complexes (COCs) , and the B4galnt2 existing on plasma membrane of cumulus cell was observed. Blocking the COC cultivation with B4galnt2 antibody resulted in disturbing of cumulus expansion, and it indicated that B4galnt2 protein is essential for cumulus expansion, which is a critical step during oogenesis. Cumulus expansion depends on both FSH in the environment and B4galnt2 protein that exist on the cell membrane of cumulus cell, and may be associated with cell migration ability. B4GALNT2 silencing of HCT116 with transfecting the plasmid contained B4GALNT2-siRNA caused the cell migration slower, but blocking with antibody made no difference, and the similar result was obtained from the two other colonic cell-CaCo2 and SW480 in antibody blocking test.
Lu, Po-Hsun, та 呂柏勳. "Functional test of β-1,4-N-acetyl-galactosaminyl transferase 2 in mouse sertoli cell". Thesis, 2013. http://ndltd.ncl.edu.tw/handle/40824461573505875544.
Повний текст джерела國立臺灣大學
生化科學研究所
101
Sda β-1,4-N-acetyl-galactosaminyl transferaseⅡis a kind of carbohydrate transferases, whose main function is to catalyze and transfer GalNAc to Gal of [Neu5Acα2-3]Galβ1-4GlcNAcβ1-3Gal through β-1,4 bond, so that carbohydrate is linked to form the Sda structure. The structure was earliest found in the antigen structure on the blood corpuscle surface; in fact, however, it is widely distributed in the tissues and body fluids of the mammals, including stomach, intestine, kidney, serum, urine, and milk. Recent studies showed that the B4GALNT2 expression was obviously reduced in the gastric cancer and colon cancer cells of human being, causing decrease in the Sda antigen, and the said reduction was closely correlated with the metastasis of the cancer cells; this indicated the importance of B4GALNT2 and its correlation with the bio-system. However, very few studies have been conducted on its physiological functions. In 2003, the research team led by Dell first found the Sda structure in the female reproductive system. Before the physiological functions of the Sda structure was learned about, its invertase B4galnt2 needed to be studied. The research showed that B4galnt2 was correlated with the mouse’s embryo implantation and the maturation of its oocytes. It was presumed that the Sda structure played an important role in the female reproductive system. Therefore, to infer the role of the Sda structure, the regulation and control by the Sda invertase should be learned. The laboratory has found B4galnt2 in the cell strains (TM4) of the Sertoli cells, so it was supposed that B4galnt2 might play a certain role in the male reproductive system, but its functions in the male reproductive system remained unknown yet. In the experiment, a male mouse was taken as the subject. The Quantitative Real-Time PCR and immune fluorescent staining method were used to confirm the existence of B4galnt2 on the cell membranes of the primary Sertoli cells and Sertoli cell strains. The observation indicated that whether the proliferation of the Sertoli cells was declined or enhanced after the cell strains were treated with follicle stimulating hormone, the B4galnt2 gene expression showed no marked difference. In this way, it was presumed that B4galnt2 did not participate in the proliferation of the Sertoli cells. By using hormone to treat the Sertoli cell strains, the Sertoli cells might be induced into the state of maturity. The maturity of the Sertoli cells could be confirmed by the mature marker genes and post-maturation physiological features. In the process of maturation, the B4galnt2 gene expression was increased. The RNAi suppress gene expression experiment showed that when the B4galnt2 expression was reduced, the maturation marker gene expression decreased accordingly. This showed that the Sertoli cells could not enter the state of maturity. To sum up, it was presumed that B4galnt2 participated in the maturation of the Sertoli cells. In addition, the experiment on the primary Sertoli cells showed that the B4galnt2 expression in the mouse coming close to the puberty was higher than that when it was born. When the immature primary Sertoli cells were induced to be mature, the B4galnt2 gene expression in them was raised. Thus, it was presumed that B4galnt2 might participate in the maturation of the Sertoli cells in the mouse.
Anderson, Nicole Marie. "Generation of Mouse Models of Human Hematopoietic Disease and their Use to Analyze Hematopoietic Development and Function." Thesis, 2012. http://hdl.handle.net/1807/33872.
Повний текст джерелаLi, Ying Shiuan, and 李映萱. "Use a Glycine N-Methyltransferase knockout mouse model to study the hepatocarcinogenesis of Aflatoxin B1." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/34479176690172174867.
Повний текст джерела國立陽明大學
公共衛生研究所
95
Primary hepatocellular carcinoma (HCC) is one of the common malignancies in the world. Its risk factors include hepatitis viruses B and C infections, dietary exposure to aflatoxins and alcoholism. Glycine N methyltransferase (GNMT) is an enzyme with multiple functions. It not only regulates the ratio of S-adenosylmethionine (SAM) to S-adenosylhomocysteine, but also serves as a folate-binding protein in the liver cytosol. Besides, we found that it can bind benzo (a) pyrene as well as aflatoxin B1 (AFB1) and reduces DNA adducts formation (Cancer Res.64:3617-3623, 2004; manuscript in preparation). Since its expression was down-regulated in HCC, the goals of this study were 1) to study the tumorigenesis effect of AFB1 in a Gnmt knockout mouse model; 2) to compare the gender differences of susceptibility to AFB1 treatment using a Gnmt knockout mouse model; 3) to analyze the gene expression profiles of the liver from animals treated with AFB1. A Gnmt knockout (KO) mouse model, developed in our laboratory was used for this study. Both male and female wildtype (Wt) and homozygous KO (Gnmt -/-) mice were challenged twice with AFB1 on the 7th day (10ug per body weight in gram) and at the 9th weeks (40ug) of age intraperitoneally. Another two groups of mice were challenged with solvent-tricaprylin. The liver tumorigenesis of all the experimental groups were regularly monitored using ultrasound (US) and magnetic resonance imaging (MRI) examinations. All eight groups of mice were sacrificed at the age of 3 and 6 months old and their liver were used for pathological studies and gene profiling analysis. The results showed that hepatomegaly was observed in the male Wt mice treated with AFB1 at 3 and 6 months of age. All Gnmt -/- mice treated with solvent had hepatomegaly at 3 and 6 months of age while only 50% (2/4) of Gnmt -/- mice treated with AFB1 had hepatomegaly at 3 months of age. For the female mice, AFB1 treatment did not induce hepatomegaly in Wt mice while both AFB1 and solvent groups of Gnmt -/- mice developed hepatomegaly at 3 and 6 months of age. Elevation of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels correlate with the hepatomegaly in different groups. At 3 months, no obvious pathological changes were observed in Wt mice in the solvent group. In animals treated with AFB1, inflammation, enlarged nuclei and hyper-chromatic staining (anisonucleosis) were observed both in Wt and Gnmt -/- mice. In addition, fatty degenerative changes were found in 5 of 6 female Gnmt -/- mice at 6 months old which was less obvious in male Gnmt -/- mice and none was observed in wildtype mice of both gender. Through using ultrasound and magnetic resonance imaging examinations, small nodules were detected in all (5/5) female and 50.0% (4/8) male Gnmt -/- mice in the treatment groups at the age of 13 -14 month. In contrast, no nodules were observed in Wt of both genders at the same age. All mice carrying nodules larger than 0.6cm were sacrificed for pathological examinations and RNA analysis. Four markers; AFP, glypican-3, survivin, and LYVE1 were also applied to confirm pathology definitions. We will continue monitoring the tumorigenesis of all the mice. In addition, the gene expression profiles of the liver from animals treated with AFB1 will be analyzed using microarray and real-time PCR in the near future.
Richard, Bernhard Clemens. "In Vitro and In Vivo Studies on Antibodies - N-terminally Truncated Abeta in the 5XFAD Mouse Model." Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0022-6000-1.
Повний текст джерелаChen, Shih-Hui, and 陳詩蕙. "Persistent Hepatitis B Viral Replication in an FVB/N Mouse Model: Impact of Host and Viral Factors." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/68204592483401377584.
Повний текст джерела國立臺灣大學
微生物學研究所
100
The mechanism underlying the chronicity of hepatitis B virus (HBV) infection has long eluded researchers. The mechanism has remained unclear largely due to the lack of an animal model that can support persistent HBV replication and allow for the investigation of the relevant immune responses. In this study, we used hydrodynamic injection to introduce HBV replicon DNA into the livers of three different mouse strains, BALB/c, C57BL/6, and FVB/N. Interestingly, we found that an HBV clone persistently replicated in the livers of FVB/N mice for up to 50 weeks but was rapidly cleared from the livers of BALB/c and C57BL/6 mice. Flow cytometric analysis and quantitative reverse transcription PCR analysis of the mouse livers indicated that after DNA injection, FVB/N mice had few intrahepatic activated cytotoxic T lymphocytes (CTLs) and produced low levels of alanine aminotransferase, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and the CXCL9 and CXCL10 chemokines. These responses were in sharp contrast to those observed in BALB/c and C57BL/6 mice, reflecting a strong correlation between the degree of liver inflammation and viral clearance. Mutational analysis further demonstrated that a change from Asn-214 to Ser-214 in the HBV surface antigen permitted the clearance of the persistent HBV clone in FVB/N mice, and the response was accompanied by increased levels of activated CTLs and upregulated liver expression of IFN-γ, CXCL9, and CXCL10. The model was demonstrated to be useful for the in vivo evaluation of the efficacies of various anti-HBV drugs. Supplementary expression of CXCL9, CXCL10 and C5a in the carrier mice enhanced the clearance of the chronic infection. These results indicate that the heterogeneity of the host factors and viral sequences may influence the immune responses against HBV. An inadequate activation of immune or inflammatory responses can lead to persistent HBV replication in vivo.
Jao, Jo-Hui, and 饒若卉. "Purification and Crystallization of the N-terminal domain of Mouse Tumor-necrosis factor receptor associated factor 6." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/d9s2qr.
Повний текст джерела國立臺北科技大學
有機高分子研究所
96
Tumor-necrosis factor (TNF) receptor-associated factor 6 (TRAF6) is an adaptor protein involved in interleukin-1 receptor (IL-1) and toll-like receptor (TLR) induced activation of nuclear factor κB (NF-kB). TRAF6 contains two domains, C- and N-terminal domains, which associate with other adaptor proteins of IL-1R/TLR pathway. Crystal structure of TRAF6 C-domain has been reported but lack of N-domain which contains a RING finger domain and several zinc finger motifs. In order to understand the protein-protein interaction, we plan to determine crystal structures of the TRAF6 N-domain residues 1~350 (TRAF6N), and the full-length TRAF6. The aim of this study was to clone DNA, protein expression, purification, and crystallization. Our results show that the E. coli expression of TRAF6N and TRAF are in suspension (70%) and inclusion body, respectively. Recombinant TRAF6N was purified by 30% ammonium sulfate salt-out method and followed a glutathione-S-transferase (GST)-affinity chromatography. TRAF6N protein was concentrated to 5.2 mg/ml and crystallized by the hanging-drop vapor diffusion method.